AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017361.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017361.01.T01 MTR_3g105930 77.528 178 7 1 1 145 1 178 1.89e-76 225
MsG0380017361.01.T01 MTR_7g090170 73.649 148 23 3 1 132 1 148 2.85e-52 163
MsG0380017361.01.T01 MTR_1g014120 69.474 95 28 1 7 100 4 98 5.12e-43 141
MsG0380017361.01.T01 MTR_4g081100 52.874 87 40 1 11 97 16 101 1.45e-24 94.7
MsG0380017361.01.T01 MTR_8g463180 45.055 91 43 3 12 98 5 92 1.47e-19 83.2
MsG0380017361.01.T01 MTR_2g083250 48.235 85 41 3 24 106 23 106 1.73e-17 77.0
MsG0380017361.01.T01 MTR_4g078410 53.333 75 32 2 24 98 22 93 2.92e-17 75.1
MsG0380017361.01.T01 MTR_4g077787 42.308 104 50 4 1 97 1 101 5.26e-17 74.3
MsG0380017361.01.T01 MTR_2g079030 48.276 87 42 2 12 98 10 93 4.33e-16 74.7
MsG0380017361.01.T01 MTR_2g090575 43.820 89 44 3 12 97 7 92 9.70e-16 73.2
MsG0380017361.01.T01 MTR_2g090580 43.750 96 43 3 10 97 2 94 1.03e-15 71.2
MsG0380017361.01.T01 MTR_2g101300 42.105 76 40 3 23 97 23 95 5.21e-15 69.3
MsG0380017361.01.T01 MTR_8g095013 44.048 84 45 2 14 97 12 93 9.58e-15 70.1
MsG0380017361.01.T01 MTR_2g088990 38.095 84 47 2 14 97 12 90 9.91e-15 68.9
MsG0380017361.01.T01 MTR_4g111640 43.434 99 43 6 8 97 7 101 1.03e-14 68.2
MsG0380017361.01.T01 MTR_8g094990 42.391 92 51 2 14 105 12 101 1.33e-14 69.3
MsG0380017361.01.T01 MTR_4g130780 34.524 84 55 0 15 98 17 100 8.15e-14 65.9
MsG0380017361.01.T01 MTR_4g124280 46.377 69 36 1 29 97 27 94 1.15e-13 66.2
MsG0380017361.01.T01 MTR_6g022170 42.857 77 40 3 23 98 30 103 4.04e-13 63.5
MsG0380017361.01.T01 MTR_4g130800 35.556 90 53 3 12 97 11 99 1.37e-12 63.9
MsG0380017361.01.T01 MTR_8g007035 38.202 89 49 3 10 98 7 89 5.09e-11 58.5
MsG0380017361.01.T01 MTR_8g007020 35.955 89 51 3 10 98 7 89 6.86e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017361.01.T01 AT4G31840 42.373 177 64 4 8 147 2 177 3.21e-37 126
MsG0380017361.01.T01 AT2G25060 40.994 161 63 2 11 139 15 175 2.21e-35 121
MsG0380017361.01.T01 AT5G25090 55.208 96 42 1 6 100 4 99 2.71e-33 116
MsG0380017361.01.T01 AT4G30590 57.609 92 34 1 12 98 8 99 1.48e-32 114
MsG0380017361.01.T01 AT2G23990 46.000 100 54 0 1 100 1 100 4.26e-26 98.6
MsG0380017361.01.T01 AT5G57920 52.632 76 36 0 22 97 18 93 2.52e-25 95.9
MsG0380017361.01.T01 AT5G57920 52.632 76 36 0 22 97 50 125 2.89e-25 96.7
MsG0380017361.01.T01 AT5G57920 52.703 74 35 0 24 97 19 92 9.74e-25 94.4
MsG0380017361.01.T01 AT2G23990 38.655 119 54 1 1 100 1 119 1.05e-21 87.4
MsG0380017361.01.T01 AT3G20570 40.698 86 49 2 12 97 15 98 6.58e-17 74.3
MsG0380017361.01.T01 AT4G28365 39.326 89 48 4 12 97 11 96 6.58e-15 68.9
MsG0380017361.01.T01 AT4G27520 42.529 87 47 2 12 98 16 99 7.78e-15 70.9
MsG0380017361.01.T01 AT3G18590 42.045 88 46 3 12 97 12 96 1.19e-14 68.2
MsG0380017361.01.T01 AT4G32490 36.000 100 56 4 3 97 2 98 2.84e-14 67.8
MsG0380017361.01.T01 AT1G48940 38.202 89 50 3 11 97 10 95 5.03e-14 66.2
MsG0380017361.01.T01 AT5G53870 40.426 94 46 4 11 97 7 97 2.75e-13 66.2
MsG0380017361.01.T01 AT5G53870 41.304 92 44 4 11 95 7 95 2.89e-13 66.2
MsG0380017361.01.T01 AT1G64640 32.990 97 61 2 4 97 6 101 4.37e-13 63.9
MsG0380017361.01.T01 AT5G14345 48.333 60 29 1 39 98 28 85 4.44e-13 63.2

Find 41 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAATCACCGCACCTAATT+AGG 0.210319 3:-95329434 MsG0380017361.01.T01:CDS
AACTGGACTTGTGACTGTTC+TGG 0.274010 3:-95329487 MsG0380017361.01.T01:CDS
TTGTTCTTGTTCCTTCTCTT+TGG 0.278533 3:-95330029 MsG0380017361.01.T01:CDS
AACTTCACTATATCCTAATT+AGG 0.307719 3:+95329421 None:intergenic
CCCATCATTGTAATGTTCAA+TGG 0.374125 3:+95329709 None:intergenic
CCATCATTGTAATGTTCAAT+GGG 0.379061 3:+95329710 None:intergenic
ACTGGACTTGTGACTGTTCT+GGG 0.401738 3:-95329486 MsG0380017361.01.T01:CDS
CTCTGCAGCTAAGGAACTAT+TGG 0.408413 3:-95330003 MsG0380017361.01.T01:CDS
CCCATTGAACATTACAATGA+TGG 0.412359 3:-95329710 MsG0380017361.01.T01:CDS
TGTGACTGTTCTGGGACTGT+TGG 0.436449 3:-95329478 MsG0380017361.01.T01:CDS
TTCAAAGTCGGCGATCATCT+TGG 0.440071 3:-95329903 MsG0380017361.01.T01:intron
GGACCTTCTTCCAACTCTGC+TGG 0.465938 3:+95329546 None:intergenic
AGTGCGCCGGTGTTGCGAAC+TGG 0.466338 3:-95329504 MsG0380017361.01.T01:CDS
CTTTGGTTTCTCTGCAGCTA+AGG 0.473136 3:-95330012 MsG0380017361.01.T01:CDS
CTGCTAGTTGGTGCAACAGC+TGG 0.474292 3:+95329525 None:intergenic
AGTGAGTCTGCTTCAGATGA+TGG 0.493397 3:+95329951 None:intergenic
TGCACCATCTCCAGCAGAGT+TGG 0.499713 3:-95329556 MsG0380017361.01.T01:CDS
TCTTCCAACTCTGCTGGAGA+TGG 0.500018 3:+95329552 None:intergenic
AACTCTGCTGGAGATGGTGC+AGG 0.510903 3:+95329558 None:intergenic
TTTGCAGTTTGGAAATATGA+TGG 0.513645 3:-95329794 MsG0380017361.01.T01:intron
AGCAGACTCACTCAACCAAT+GGG 0.514967 3:-95329940 MsG0380017361.01.T01:CDS
AACACCGGCGCACTGCTAGT+TGG 0.529681 3:+95329513 None:intergenic
TCTCCAAAGAAAAGATCCAT+TGG 0.532263 3:-95329588 MsG0380017361.01.T01:intron
CCATTGAACATTACAATGAT+GGG 0.540182 3:-95329709 MsG0380017361.01.T01:CDS
AAGCAGACTCACTCAACCAA+TGG 0.548958 3:-95329941 MsG0380017361.01.T01:CDS
CAGTTTGGAAATATGATGGT+GGG 0.580211 3:-95329790 MsG0380017361.01.T01:CDS
TGCACCAACTAGCAGTGCGC+CGG 0.584450 3:-95329517 MsG0380017361.01.T01:CDS
GAAAAGTCTCGTTTCAAAGT+CGG 0.587560 3:-95329915 MsG0380017361.01.T01:CDS
TCTCCAGCAGAGTTGGAAGA+AGG 0.588093 3:-95329549 MsG0380017361.01.T01:CDS
ACAAGTCCAGTTCGCAACAC+CGG 0.589779 3:+95329498 None:intergenic
TGGTGCAGGTGAAACTCCAA+TGG 0.600203 3:+95329572 None:intergenic
GCAGTTTGGAAATATGATGG+TGG 0.604799 3:-95329791 MsG0380017361.01.T01:intron
CACTATATCCTAATTAGGTG+CGG 0.608986 3:+95329426 None:intergenic
AGGCAAGATAGATGCATGGA+AGG 0.615286 3:-95329976 MsG0380017361.01.T01:CDS
CTGCAGAGAAACCAAAGAGA+AGG 0.616684 3:+95330018 None:intergenic
CATCATTGTAATGTTCAATG+GGG 0.626069 3:+95329711 None:intergenic
GCAGCTAAGGAACTATTGGT+CGG 0.634929 3:-95329999 MsG0380017361.01.T01:CDS
AGTTCTTGAGAACACAGCCA+TGG 0.674329 3:+95330067 None:intergenic
TCGGAGGCAAGATAGATGCA+TGG 0.675253 3:-95329980 MsG0380017361.01.T01:CDS
GCTAAGGAACTATTGGTCGG+AGG 0.690687 3:-95329996 MsG0380017361.01.T01:CDS
TTACAATGATGGGAACACGA+AGG 0.756088 3:-95329699 MsG0380017361.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATTTCCAAACTGCAAAACAA+AGG + Chr3:95329695-95329714 None:intergenic 30.0%
CATCATTGTAATGTTCAATG+GGG + Chr3:95329785-95329804 None:intergenic 30.0%
CCATCATTGTAATGTTCAAT+GGG + Chr3:95329786-95329805 None:intergenic 30.0%
CCATTGAACATTACAATGAT+GGG - Chr3:95329784-95329803 MsG0380017361.01.T01:CDS 30.0%
TTTGCAGTTTGGAAATATGA+TGG - Chr3:95329699-95329718 MsG0380017361.01.T01:intron 30.0%
!!! TTTACCTTTGTTTTGCAGTT+TGG - Chr3:95329688-95329707 MsG0380017361.01.T01:intron 30.0%
AGAAAAAGATGTGTCAGTGA+TGG + Chr3:95329669-95329688 None:intergenic 35.0%
CAGTTTGGAAATATGATGGT+GGG - Chr3:95329703-95329722 MsG0380017361.01.T01:CDS 35.0%
CCCATCATTGTAATGTTCAA+TGG + Chr3:95329787-95329806 None:intergenic 35.0%
CCCATTGAACATTACAATGA+TGG - Chr3:95329783-95329802 MsG0380017361.01.T01:CDS 35.0%
GAAAAGTCTCGTTTCAAAGT+CGG - Chr3:95329578-95329597 MsG0380017361.01.T01:CDS 35.0%
TCTCCAAAGAAAAGATCCAT+TGG - Chr3:95329905-95329924 MsG0380017361.01.T01:CDS 35.0%
TTGTTCTTGTTCCTTCTCTT+TGG - Chr3:95329464-95329483 MsG0380017361.01.T01:CDS 35.0%
! ACTCCAATGGATCTTTTCTT+TGG + Chr3:95329911-95329930 None:intergenic 35.0%
ATGAATCACCGCACCTAATT+AGG - Chr3:95330059-95330078 MsG0380017361.01.T01:CDS 40.0%
CTGATGAAGTAAAATGGTCC+AGG + Chr3:95329834-95329853 None:intergenic 40.0%
GCAGTTTGGAAATATGATGG+TGG - Chr3:95329702-95329721 MsG0380017361.01.T01:CDS 40.0%
GCTCCACTGATGAAGTAAAA+TGG + Chr3:95329840-95329859 None:intergenic 40.0%
TTACTTCATCAGTGGAGCAA+AGG - Chr3:95329842-95329861 MsG0380017361.01.T01:intron 40.0%
! GGACCATTTTACTTCATCAG+TGG - Chr3:95329834-95329853 MsG0380017361.01.T01:intron 40.0%
! TTACAATGATGGGAACACGA+AGG - Chr3:95329794-95329813 MsG0380017361.01.T01:intron 40.0%
AACTGGACTTGTGACTGTTC+TGG - Chr3:95330006-95330025 MsG0380017361.01.T01:CDS 45.0%
AAGCAGACTCACTCAACCAA+TGG - Chr3:95329552-95329571 MsG0380017361.01.T01:CDS 45.0%
AAGGTAAAGCTAGACAGACC+TGG - Chr3:95329813-95329832 MsG0380017361.01.T01:intron 45.0%
ACTGGACTTGTGACTGTTCT+GGG - Chr3:95330007-95330026 MsG0380017361.01.T01:CDS 45.0%
AGCAGACTCACTCAACCAAT+GGG - Chr3:95329553-95329572 MsG0380017361.01.T01:CDS 45.0%
AGGACAAAAGCTGATTGTGG+TGG - Chr3:95329877-95329896 MsG0380017361.01.T01:intron 45.0%
AGGCAAGATAGATGCATGGA+AGG - Chr3:95329517-95329536 MsG0380017361.01.T01:CDS 45.0%
AGTGAGTCTGCTTCAGATGA+TGG + Chr3:95329545-95329564 None:intergenic 45.0%
CTCTGCAGCTAAGGAACTAT+TGG - Chr3:95329490-95329509 MsG0380017361.01.T01:CDS 45.0%
CTGCAGAGAAACCAAAGAGA+AGG + Chr3:95329478-95329497 None:intergenic 45.0%
GCAAGGACAAAAGCTGATTG+TGG - Chr3:95329874-95329893 MsG0380017361.01.T01:intron 45.0%
GCAGCTAAGGAACTATTGGT+CGG - Chr3:95329494-95329513 MsG0380017361.01.T01:CDS 45.0%
TTCAAAGTCGGCGATCATCT+TGG - Chr3:95329590-95329609 MsG0380017361.01.T01:intron 45.0%
! AACGAGACTTTTCTGCCCAT+TGG + Chr3:95329571-95329590 None:intergenic 45.0%
! CTTTGGTTTCTCTGCAGCTA+AGG - Chr3:95329481-95329500 MsG0380017361.01.T01:CDS 45.0%
!!! GGGACTGTTGGCTTTTATGT+GGG - Chr3:95330027-95330046 MsG0380017361.01.T01:CDS 45.0%
!!! TGGGACTGTTGGCTTTTATG+TGG - Chr3:95330026-95330045 MsG0380017361.01.T01:CDS 45.0%
ACAAGTCCAGTTCGCAACAC+CGG + Chr3:95329998-95330017 None:intergenic 50.0%
AGCAAAGGACACTGTGAGCA+AGG - Chr3:95329857-95329876 MsG0380017361.01.T01:intron 50.0%
GCTAAGGAACTATTGGTCGG+AGG - Chr3:95329497-95329516 MsG0380017361.01.T01:CDS 50.0%
TCGGAGGCAAGATAGATGCA+TGG - Chr3:95329513-95329532 MsG0380017361.01.T01:CDS 50.0%
TCTCCAGCAGAGTTGGAAGA+AGG - Chr3:95329944-95329963 MsG0380017361.01.T01:CDS 50.0%
TCTTCCAACTCTGCTGGAGA+TGG + Chr3:95329944-95329963 None:intergenic 50.0%
TGGTGCAGGTGAAACTCCAA+TGG + Chr3:95329924-95329943 None:intergenic 50.0%
!! TGTGACTGTTCTGGGACTGT+TGG - Chr3:95330015-95330034 MsG0380017361.01.T01:CDS 50.0%
AACTCTGCTGGAGATGGTGC+AGG + Chr3:95329938-95329957 None:intergenic 55.0%
GGACCTTCTTCCAACTCTGC+TGG + Chr3:95329950-95329969 None:intergenic 55.0%
TGCACCATCTCCAGCAGAGT+TGG - Chr3:95329937-95329956 MsG0380017361.01.T01:CDS 55.0%
! CTGCTAGTTGGTGCAACAGC+TGG + Chr3:95329971-95329990 None:intergenic 55.0%
AACACCGGCGCACTGCTAGT+TGG + Chr3:95329983-95330002 None:intergenic 60.0%
TGCACCAACTAGCAGTGCGC+CGG - Chr3:95329976-95329995 MsG0380017361.01.T01:CDS 60.0%
!! AGTGCGCCGGTGTTGCGAAC+TGG - Chr3:95329989-95330008 MsG0380017361.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 95329428 95330087 95329428 ID=MsG0380017361.01;Name=MsG0380017361.01
Chr3 mRNA 95329428 95330087 95329428 ID=MsG0380017361.01.T01;Parent=MsG0380017361.01;Name=MsG0380017361.01.T01;_AED=0.50;_eAED=0.53;_QI=0|0|0|1|1|1|3|0|155
Chr3 exon 95329904 95330087 95329904 ID=MsG0380017361.01.T01:exon:1652;Parent=MsG0380017361.01.T01
Chr3 exon 95329700 95329809 95329700 ID=MsG0380017361.01.T01:exon:1651;Parent=MsG0380017361.01.T01
Chr3 exon 95329428 95329601 95329428 ID=MsG0380017361.01.T01:exon:1650;Parent=MsG0380017361.01.T01
Chr3 CDS 95329904 95330087 95329904 ID=MsG0380017361.01.T01:cds;Parent=MsG0380017361.01.T01
Chr3 CDS 95329700 95329809 95329700 ID=MsG0380017361.01.T01:cds;Parent=MsG0380017361.01.T01
Chr3 CDS 95329428 95329601 95329428 ID=MsG0380017361.01.T01:cds;Parent=MsG0380017361.01.T01
Gene Sequence

>MsG0380017361.01.T01

ATGGCTGTGTTCTCAAGAACTGCTTCTGCTTCTCTATTGTTCTTGTTCCTTCTCTTTGGTTTCTCTGCAGCTAAGGAACTATTGGTCGGAGGCAAGATAGATGCATGGAAGGTTCCATCATCTGAAGCAGACTCACTCAACCAATGGGCAGAAAAGTCTCGTTTCAAAGTCGGCGATCATCTTGTTTGGAAATATGATGGTGGGAAAGACTCAGTTTTGCAAGTAAACAAAGAAGATTATGCTAATTGCAACAGCTCAAACCCCATTGAACATTACAATGATGGGAACACGAAGAAAAGATCCATTGGAGTTTCACCTGCACCATCTCCAGCAGAGTTGGAAGAAGGTCCAGCTGTTGCACCAACTAGCAGTGCGCCGGTGTTGCGAACTGGACTTGTGACTGTTCTGGGACTGTTGGCTTTTATGTGGGTTTTCTCATATGAATCACCGCACCTAATTAGGATATAG

Protein sequence

>MsG0380017361.01.T01

MAVFSRTASASLLFLFLLFGFSAAKELLVGGKIDAWKVPSSEADSLNQWAEKSRFKVGDHLVWKYDGGKDSVLQVNKEDYANCNSSNPIEHYNDGNTKKRSIGVSPAPSPAELEEGPAVAPTSSAPVLRTGLVTVLGLLAFMWVFSYESPHLIRI*