Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046804.01.T01 | XP_003609384.2 | 98.726 | 157 | 2 | 0 | 1 | 157 | 174 | 330 | 1.75E-110 | 329 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046804.01.T01 | G7JE73 | 98.726 | 157 | 2 | 0 | 1 | 157 | 174 | 330 | 8.36e-111 | 329 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002276.01 | MsG0880046804.01 | -0.804338 | 2.266830e-49 | 1.165760e-46 |
| MsG0880046165.01 | MsG0880046804.01 | -0.828006 | 1.142656e-54 | 1.121600e-51 |
| MsG0680034392.01 | MsG0880046804.01 | -0.811689 | 6.175966e-51 | 3.848113e-48 |
| MsG0280009241.01 | MsG0880046804.01 | -0.803012 | 4.270984e-49 | 2.122799e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046804.01.T01 | MTR_4g115070 | 98.726 | 157 | 2 | 0 | 1 | 157 | 174 | 330 | 2.12e-114 | 329 |
| MsG0880046804.01.T01 | MTR_7g033325 | 62.658 | 158 | 58 | 1 | 1 | 157 | 197 | 354 | 1.34e-69 | 216 |
| MsG0880046804.01.T01 | MTR_7g033325 | 62.658 | 158 | 58 | 1 | 1 | 157 | 196 | 353 | 1.40e-69 | 216 |
| MsG0880046804.01.T01 | MTR_8g089840 | 59.494 | 158 | 63 | 1 | 1 | 157 | 202 | 359 | 9.88e-66 | 206 |
| MsG0880046804.01.T01 | MTR_3g096150 | 58.861 | 158 | 64 | 1 | 1 | 157 | 177 | 334 | 1.73e-65 | 204 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046804.01.T01 | AT2G13290 | 72.152 | 158 | 43 | 1 | 1 | 157 | 172 | 329 | 6.78e-84 | 251 |
| MsG0880046804.01.T01 | AT2G13290 | 72.152 | 158 | 43 | 1 | 1 | 157 | 172 | 329 | 6.78e-84 | 251 |
| MsG0880046804.01.T01 | AT3G27540 | 62.025 | 158 | 59 | 1 | 1 | 157 | 196 | 353 | 4.38e-72 | 222 |
| MsG0880046804.01.T01 | AT5G14480 | 60.759 | 158 | 61 | 1 | 1 | 157 | 197 | 354 | 1.03e-69 | 216 |
| MsG0880046804.01.T01 | AT1G12990 | 63.291 | 158 | 57 | 1 | 1 | 157 | 202 | 359 | 5.58e-68 | 211 |
| MsG0880046804.01.T01 | AT1G67880 | 61.392 | 158 | 60 | 1 | 1 | 157 | 200 | 357 | 5.71e-68 | 211 |
| MsG0880046804.01.T01 | AT3G01620 | 58.861 | 158 | 64 | 1 | 1 | 157 | 198 | 355 | 1.82e-67 | 210 |
| MsG0880046804.01.T01 | AT3G26445 | 52.239 | 67 | 31 | 1 | 13 | 78 | 47 | 113 | 2.40e-18 | 76.6 |
Find 46 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCAACTCAAGATGCATTA+TGG | 0.224726 | 8:+78444554 | None:intergenic |
| GCCCGAAGAATACTCCTTTC+AGG | 0.283433 | 8:-78444253 | MsG0880046804.01.T01:CDS |
| GATCATCTCCCTTGCAAATA+AGG | 0.299752 | 8:+78444289 | None:intergenic |
| CTCAACTCAAGATGCATTAT+GGG | 0.348786 | 8:+78444555 | None:intergenic |
| CTTGAATTCTTGAATGACTA+AGG | 0.393185 | 8:+78444313 | None:intergenic |
| CTCCTGAAAGGAGTATTCTT+CGG | 0.405596 | 8:+78444251 | None:intergenic |
| TCCTGAAAGGAGTATTCTTC+GGG | 0.415421 | 8:+78444252 | None:intergenic |
| ACGGTCCCCGGTCTAATTAC+CGG | 0.446231 | 8:-78444467 | MsG0880046804.01.T01:CDS |
| CGCCAGCTGTTGTAGTCCAC+AGG | 0.447034 | 8:+78444507 | None:intergenic |
| ACGGTGTAACAATGTATTCA+TGG | 0.463984 | 8:+78444677 | None:intergenic |
| TGAAGAAGTTTCAAAGTATG+AGG | 0.469432 | 8:+78444597 | None:intergenic |
| AGTTTCAAAGTATGAGGACT+TGG | 0.479637 | 8:+78444603 | None:intergenic |
| AATTCAAGACCTTATTTGCA+AGG | 0.500490 | 8:-78444298 | MsG0880046804.01.T01:CDS |
| CTGAGAAGATAACATCGGTC+TGG | 0.501527 | 8:+78444436 | None:intergenic |
| GCCACGCCGACCGTGTGAAA+CGG | 0.512900 | 8:-78444344 | MsG0880046804.01.T01:CDS |
| AAACTTCTTCAGTGGTGCGA+TGG | 0.521118 | 8:-78444585 | MsG0880046804.01.T01:CDS |
| CATCGGTCTGGCGCGAATGC+CGG | 0.524753 | 8:+78444448 | None:intergenic |
| CGAATGCCGGTAATTAGACC+GGG | 0.532797 | 8:+78444461 | None:intergenic |
| GCGAATGCCGGTAATTAGAC+CGG | 0.534546 | 8:+78444460 | None:intergenic |
| AATACATTGTTACACCGTGC+GGG | 0.535414 | 8:-78444672 | MsG0880046804.01.T01:CDS |
| ATTCAAGACCTTATTTGCAA+GGG | 0.538163 | 8:-78444297 | MsG0880046804.01.T01:CDS |
| ATACTTTGAAACTTCTTCAG+TGG | 0.541887 | 8:-78444593 | MsG0880046804.01.T01:CDS |
| CAGCCCATGTCTACGGTCCC+CGG | 0.543615 | 8:-78444479 | MsG0880046804.01.T01:CDS |
| CGAGCGACAGCCCATGTCTA+CGG | 0.544052 | 8:-78444486 | MsG0880046804.01.T01:CDS |
| CGGTGATATTCTTCTAATGT+CGG | 0.554646 | 8:-78444640 | MsG0880046804.01.T01:CDS |
| CAATACTCGTTCAGCTTCTG+CGG | 0.556408 | 8:-78444209 | MsG0880046804.01.T01:CDS |
| CACCGTGCGGGGATTTCCAA+CGG | 0.563483 | 8:-78444660 | MsG0880046804.01.T01:CDS |
| TCCCTGTGGACTACAACAGC+TGG | 0.568994 | 8:-78444509 | MsG0880046804.01.T01:CDS |
| TTATCTTCTCAGACGCGGGA+TGG | 0.582193 | 8:-78444425 | MsG0880046804.01.T01:CDS |
| GATGTTATCTTCTCAGACGC+GGG | 0.583396 | 8:-78444429 | MsG0880046804.01.T01:CDS |
| TAGACCGGGGACCGTAGACA+TGG | 0.590389 | 8:+78444475 | None:intergenic |
| CATTAGAAGAATATCACCGT+TGG | 0.601464 | 8:+78444644 | None:intergenic |
| GGAAAGACCTCCGTTTCACA+CGG | 0.611357 | 8:+78444334 | None:intergenic |
| AGACCTCCGTTTCACACGGT+CGG | 0.622233 | 8:+78444338 | None:intergenic |
| TCCGTTTCACACGGTCGGCG+TGG | 0.626436 | 8:+78444343 | None:intergenic |
| ATACATTGTTACACCGTGCG+GGG | 0.628594 | 8:-78444671 | MsG0880046804.01.T01:CDS |
| AACTCAAGATGCATTATGGG+AGG | 0.632104 | 8:+78444558 | None:intergenic |
| CGATGTTATCTTCTCAGACG+CGG | 0.644839 | 8:-78444430 | MsG0880046804.01.T01:CDS |
| GTATTCATTTGAGTTCCCTG+TGG | 0.649334 | 8:-78444523 | MsG0880046804.01.T01:CDS |
| ACGCCGACCGTGTGAAACGG+AGG | 0.662932 | 8:-78444341 | MsG0880046804.01.T01:CDS |
| AGACCGGGGACCGTAGACAT+GGG | 0.678926 | 8:+78444476 | None:intergenic |
| GCCAGCTGTTGTAGTCCACA+GGG | 0.679316 | 8:+78444508 | None:intergenic |
| CACCGTTGGAAATCCCCGCA+CGG | 0.683745 | 8:+78444658 | None:intergenic |
| GAATACATTGTTACACCGTG+CGG | 0.705399 | 8:-78444673 | MsG0880046804.01.T01:CDS |
| CGCGTCTGAGAAGATAACAT+CGG | 0.723208 | 8:+78444431 | None:intergenic |
| GAATGCCGGTAATTAGACCG+GGG | 0.739409 | 8:+78444462 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTTGAATTCTTGAATGACTA+AGG | + | Chr8:78444553-78444572 | None:intergenic | 30.0% | |
| TGAAGAAGTTTCAAAGTATG+AGG | + | Chr8:78444269-78444288 | None:intergenic | 30.0% | |
| ! | AATTCAAGACCTTATTTGCA+AGG | - | Chr8:78444565-78444584 | MsG0880046804.01.T01:CDS | 30.0% |
| ! | AGGAGTTGATTAAAAAGATG+GGG | - | Chr8:78444630-78444649 | MsG0880046804.01.T01:CDS | 30.0% |
| ! | ATTCAAGACCTTATTTGCAA+GGG | - | Chr8:78444566-78444585 | MsG0880046804.01.T01:CDS | 30.0% |
| ! | CAGGAGTTGATTAAAAAGAT+GGG | - | Chr8:78444629-78444648 | MsG0880046804.01.T01:CDS | 30.0% |
| ! | CTTTTTAATCAACTCCTGAA+AGG | + | Chr8:78444627-78444646 | None:intergenic | 30.0% |
| ! | TCAGGAGTTGATTAAAAAGA+TGG | - | Chr8:78444628-78444647 | MsG0880046804.01.T01:CDS | 30.0% |
| !! | ATACTTTGAAACTTCTTCAG+TGG | - | Chr8:78444270-78444289 | MsG0880046804.01.T01:CDS | 30.0% |
| AGTTTCAAAGTATGAGGACT+TGG | + | Chr8:78444263-78444282 | None:intergenic | 35.0% | |
| CATTAGAAGAATATCACCGT+TGG | + | Chr8:78444222-78444241 | None:intergenic | 35.0% | |
| CGGTGATATTCTTCTAATGT+CGG | - | Chr8:78444223-78444242 | MsG0880046804.01.T01:CDS | 35.0% | |
| CTCAACTCAAGATGCATTAT+GGG | + | Chr8:78444311-78444330 | None:intergenic | 35.0% | |
| TCTCAACTCAAGATGCATTA+TGG | + | Chr8:78444312-78444331 | None:intergenic | 35.0% | |
| ! | GGCATTGTAGTTTTTGCTTT+CGG | - | Chr8:78444459-78444478 | MsG0880046804.01.T01:CDS | 35.0% |
| AACTCAAGATGCATTATGGG+AGG | + | Chr8:78444308-78444327 | None:intergenic | 40.0% | |
| AATACATTGTTACACCGTGC+GGG | - | Chr8:78444191-78444210 | MsG0880046804.01.T01:CDS | 40.0% | |
| GAATACATTGTTACACCGTG+CGG | - | Chr8:78444190-78444209 | MsG0880046804.01.T01:CDS | 40.0% | |
| GATCATCTCCCTTGCAAATA+AGG | + | Chr8:78444577-78444596 | None:intergenic | 40.0% | |
| ! | GTATTCATTTGAGTTCCCTG+TGG | - | Chr8:78444340-78444359 | MsG0880046804.01.T01:CDS | 40.0% |
| !! | CTCCTGAAAGGAGTATTCTT+CGG | + | Chr8:78444615-78444634 | None:intergenic | 40.0% |
| !! | TCCTGAAAGGAGTATTCTTC+GGG | + | Chr8:78444614-78444633 | None:intergenic | 40.0% |
| AAACTTCTTCAGTGGTGCGA+TGG | - | Chr8:78444278-78444297 | MsG0880046804.01.T01:CDS | 45.0% | |
| ATACATTGTTACACCGTGCG+GGG | - | Chr8:78444192-78444211 | MsG0880046804.01.T01:CDS | 45.0% | |
| CAATACTCGTTCAGCTTCTG+CGG | - | Chr8:78444654-78444673 | MsG0880046804.01.T01:CDS | 45.0% | |
| CGATGTTATCTTCTCAGACG+CGG | - | Chr8:78444433-78444452 | MsG0880046804.01.T01:CDS | 45.0% | |
| CGCGTCTGAGAAGATAACAT+CGG | + | Chr8:78444435-78444454 | None:intergenic | 45.0% | |
| CTGAGAAGATAACATCGGTC+TGG | + | Chr8:78444430-78444449 | None:intergenic | 45.0% | |
| GATGTTATCTTCTCAGACGC+GGG | - | Chr8:78444434-78444453 | MsG0880046804.01.T01:CDS | 45.0% | |
| CGAATGCCGGTAATTAGACC+GGG | + | Chr8:78444405-78444424 | None:intergenic | 50.0% | |
| GAATGCCGGTAATTAGACCG+GGG | + | Chr8:78444404-78444423 | None:intergenic | 50.0% | |
| GCCCGAAGAATACTCCTTTC+AGG | - | Chr8:78444610-78444629 | MsG0880046804.01.T01:CDS | 50.0% | |
| GCGAATGCCGGTAATTAGAC+CGG | + | Chr8:78444406-78444425 | None:intergenic | 50.0% | |
| GGAAAGACCTCCGTTTCACA+CGG | + | Chr8:78444532-78444551 | None:intergenic | 50.0% | |
| TTATCTTCTCAGACGCGGGA+TGG | - | Chr8:78444438-78444457 | MsG0880046804.01.T01:CDS | 50.0% | |
| ACGGTCCCCGGTCTAATTAC+CGG | - | Chr8:78444396-78444415 | MsG0880046804.01.T01:CDS | 55.0% | |
| AGACCTCCGTTTCACACGGT+CGG | + | Chr8:78444528-78444547 | None:intergenic | 55.0% | |
| GCCAGCTGTTGTAGTCCACA+GGG | + | Chr8:78444358-78444377 | None:intergenic | 55.0% | |
| ! | TCCCTGTGGACTACAACAGC+TGG | - | Chr8:78444354-78444373 | MsG0880046804.01.T01:CDS | 55.0% |
| AGACCGGGGACCGTAGACAT+GGG | + | Chr8:78444390-78444409 | None:intergenic | 60.0% | |
| CACCGTGCGGGGATTTCCAA+CGG | - | Chr8:78444203-78444222 | MsG0880046804.01.T01:CDS | 60.0% | |
| CACCGTTGGAAATCCCCGCA+CGG | + | Chr8:78444208-78444227 | None:intergenic | 60.0% | |
| CGAGCGACAGCCCATGTCTA+CGG | - | Chr8:78444377-78444396 | MsG0880046804.01.T01:CDS | 60.0% | |
| CGCCAGCTGTTGTAGTCCAC+AGG | + | Chr8:78444359-78444378 | None:intergenic | 60.0% | |
| TAGACCGGGGACCGTAGACA+TGG | + | Chr8:78444391-78444410 | None:intergenic | 60.0% | |
| ACGCCGACCGTGTGAAACGG+AGG | - | Chr8:78444522-78444541 | MsG0880046804.01.T01:CDS | 65.0% | |
| CAGCCCATGTCTACGGTCCC+CGG | - | Chr8:78444384-78444403 | MsG0880046804.01.T01:CDS | 65.0% | |
| CATCGGTCTGGCGCGAATGC+CGG | + | Chr8:78444418-78444437 | None:intergenic | 65.0% | |
| GCCACGCCGACCGTGTGAAA+CGG | - | Chr8:78444519-78444538 | MsG0880046804.01.T01:CDS | 65.0% | |
| TCCGTTTCACACGGTCGGCG+TGG | + | Chr8:78444523-78444542 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 78444188 | 78444697 | 78444188 | ID=MsG0880046804.01;Name=MsG0880046804.01 |
| Chr8 | mRNA | 78444188 | 78444697 | 78444188 | ID=MsG0880046804.01.T01;Parent=MsG0880046804.01;Name=MsG0880046804.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|169 |
| Chr8 | exon | 78444188 | 78444697 | 78444188 | ID=MsG0880046804.01.T01:exon:18780;Parent=MsG0880046804.01.T01 |
| Chr8 | CDS | 78444188 | 78444697 | 78444188 | ID=MsG0880046804.01.T01:cds;Parent=MsG0880046804.01.T01 |
| Gene Sequence |
| Protein sequence |
