AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002276.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002276.01.T01 MTR_0027s0090 91.549 142 8 3 1 141 1 139 1.03e-70 218
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 116 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTCGCTTGGTAGCATTTA+AGG 0.118919 1:+36135286 MsG0180002276.01.T01:three_prime_UTR
GAGAAAATGTGAAGGGATTT+TGG 0.181897 1:-36133253 None:intergenic
TTGGTAGCATTTAAGGGATT+TGG 0.226503 1:+36135293 MsG0180002276.01.T01:three_prime_UTR
CTATATAGTCTTCAAGCTTT+AGG 0.234041 1:-36134821 None:intergenic
CTCTCCCATGTTTCGCATTT+CGG 0.242451 1:+36133167 MsG0180002276.01.T01:five_prime_UTR
CAATCAACCCTATGTGCTTT+TGG 0.244653 1:+36133735 MsG0180002276.01.T01:CDS
CTGATAGGCTGAACGCAATA+AGG 0.267631 1:+36134116 MsG0180002276.01.T01:three_prime_UTR
GTCTTCAAGCTTTAGGTTAA+GGG 0.268229 1:-36134814 None:intergenic
TGGTAGCATTTAAGGGATTT+GGG 0.283885 1:+36135294 MsG0180002276.01.T01:three_prime_UTR
TTGCTCACTGCTGCTGCTGC+TGG 0.298200 1:-36135195 None:intergenic
TCCACCTCTAATGTTGAAAA+TGG 0.300073 1:+36134881 MsG0180002276.01.T01:three_prime_UTR
TGCTGCTGGCTGAGGCCTGC+TGG 0.300278 1:-36135181 None:intergenic
CTGTAATACCACTGCAAATC+TGG 0.300724 1:-36135679 None:intergenic
AGTCTTCAAGCTTTAGGTTA+AGG 0.313871 1:-36134815 None:intergenic
TAATAATAATAGAAGATAAA+TGG 0.327212 1:+36135412 MsG0180002276.01.T01:three_prime_UTR
AACAACAACGTTGCTGGTTT+AGG 0.336273 1:+36134223 MsG0180002276.01.T01:three_prime_UTR
GCATTCTCACAAACACCATT+AGG 0.337817 1:-36133801 None:intergenic
ATTTGGATGACGGTGAGTTC+TGG 0.349881 1:+36133342 MsG0180002276.01.T01:CDS
TAGGTTGTGGAGTTTGAATT+TGG 0.351854 1:-36134291 None:intergenic
GGGGTATGCAGAAGCCAAAA+TGG 0.360070 1:+36134140 MsG0180002276.01.T01:three_prime_UTR
TGTTTCCTTGGAAATGGTTA+TGG 0.366566 1:+36134323 MsG0180002276.01.T01:three_prime_UTR
GTTATGATTCTCAGAGGCTT+TGG 0.379710 1:-36133612 None:intergenic
ATTCGCTTGGTAGCATTTAA+GGG 0.380143 1:+36135287 MsG0180002276.01.T01:three_prime_UTR
AGGAAATTGAAGGGAAAGAA+AGG 0.392309 1:-36133278 None:intergenic
CTGTTGCACCTCCAAGGCAT+AGG 0.394744 1:+36135115 MsG0180002276.01.T01:three_prime_UTR
GAGAGCTGTTTCCTTGGAAA+TGG 0.398134 1:+36134317 MsG0180002276.01.T01:three_prime_UTR
GTTGAATTTCTTGATTCAAC+AGG 0.398365 1:-36134379 None:intergenic
GTTTCGCATTTCGGTAATCA+TGG 0.406161 1:+36133176 MsG0180002276.01.T01:five_prime_UTR
TCAAGCCAAGGTAGCCCTAA+TGG 0.411212 1:+36133786 MsG0180002276.01.T01:CDS
AGCGAAAGCGAAGAAGGTTT+CGG 0.413750 1:-36133419 None:intergenic
GGGTATGCAGAAGCCAAAAT+GGG 0.423311 1:+36134141 MsG0180002276.01.T01:three_prime_UTR
AATAATAATAAATTGGTTTG+TGG 0.433439 1:+36135625 MsG0180002276.01.T01:three_prime_UTR
CTACATGAGAGCTGTTTCCT+TGG 0.435569 1:+36134311 MsG0180002276.01.T01:three_prime_UTR
GCAGCAGCAACAATACCAGC+AGG 0.436574 1:+36135166 MsG0180002276.01.T01:three_prime_UTR
AGGGCATGCGCCACTCTAGC+TGG 0.436782 1:-36134795 None:intergenic
TGATAGGCTGAACGCAATAA+GGG 0.441364 1:+36134117 MsG0180002276.01.T01:three_prime_UTR
TATGGTAGCAGCACTGGCAC+TGG 0.446098 1:+36134341 MsG0180002276.01.T01:three_prime_UTR
AGGGGATTTGATGTAACAAA+AGG 0.449526 1:+36135326 MsG0180002276.01.T01:three_prime_UTR
CATTCTCACAAACACCATTA+GGG 0.451029 1:-36133800 None:intergenic
AAATAATGTTAGGAGCCTCT+TGG 0.453899 1:+36135457 MsG0180002276.01.T01:three_prime_UTR
GGGGAAGAAGAATTTCTTTG+AGG 0.454444 1:-36133902 None:intergenic
AAAGCTTGAAGACTATATAG+TGG 0.470534 1:+36134824 MsG0180002276.01.T01:three_prime_UTR
ACATTATTACCCCTATGCCT+TGG 0.482077 1:-36135126 None:intergenic
TTGAAAAGCGAAAGCGAAGA+AGG 0.482963 1:-36133425 None:intergenic
AATGGTTATGGTAGCAGCAC+TGG 0.484581 1:+36134335 MsG0180002276.01.T01:three_prime_UTR
GGAAATTGAAGGGAAAGAAA+GGG 0.486521 1:-36133277 None:intergenic
GGAGGAAGAAATGTTGTTGA+TGG 0.488016 1:+36134190 MsG0180002276.01.T01:three_prime_UTR
TTATTACCCCTATGCCTTGG+AGG 0.489609 1:-36135123 None:intergenic
AACAGCTCTCATGTAGGTTG+TGG 0.495869 1:-36134304 None:intergenic
GGTATGCAGAAGCCAAAATG+GGG 0.497372 1:+36134142 MsG0180002276.01.T01:three_prime_UTR
TGTTGCACCTCCAAGGCATA+GGG 0.498023 1:+36135116 MsG0180002276.01.T01:three_prime_UTR
TGTTGAGCATGTTGTAGTGT+TGG 0.499882 1:-36134265 None:intergenic
GTCATTTCCAAAAGCACATA+GGG 0.500818 1:-36133742 None:intergenic
TCAGAACAAGCGAATCCATA+AGG 0.503685 1:-36133473 None:intergenic
TTATTCATAGCAAGTAACTA+AGG 0.504465 1:-36135728 None:intergenic
ATGATGAAAATGATATGAAA+TGG 0.513671 1:+36135257 MsG0180002276.01.T01:three_prime_UTR
AAACACCATTAGGGCTACCT+TGG 0.514969 1:-36133791 None:intergenic
AGCAAGTAACTAAGGTGGAC+AGG 0.516303 1:-36135720 None:intergenic
AAATGGTCCTTTGATTCGCT+TGG 0.517299 1:+36135274 MsG0180002276.01.T01:three_prime_UTR
TGTAGCTTGTGCAACTGCAT+TGG 0.518913 1:+36134770 MsG0180002276.01.T01:intron
TTTCGCATTTCGGTAATCAT+GGG 0.519762 1:+36133177 MsG0180002276.01.T01:five_prime_UTR
TAGGTTTGTCATCATCTGCA+TGG 0.523719 1:+36134242 MsG0180002276.01.T01:three_prime_UTR
GATTACCGAAATGCGAAACA+TGG 0.528517 1:-36133172 None:intergenic
GTCATCCAAATTCTCAGCCA+TGG 0.531206 1:-36133330 None:intergenic
CAATGAAATCAAGCTTCCAC+AGG 0.538105 1:+36135215 MsG0180002276.01.T01:three_prime_UTR
GAATCATAACAACAATAACT+TGG 0.540471 1:+36133625 MsG0180002276.01.T01:CDS
GGCAACAACAAATTGCTGAT+AGG 0.541898 1:+36134101 MsG0180002276.01.T01:three_prime_UTR
GAAGCAGCAAGAGAATCAAT+TGG 0.541993 1:-36133506 None:intergenic
ATGGTAGCAGCACTGGCACT+GGG 0.549013 1:+36134342 MsG0180002276.01.T01:three_prime_UTR
AAGCTTGAAGACTATATAGT+GGG 0.550779 1:+36134825 MsG0180002276.01.T01:three_prime_UTR
TGTCATTTCCAAAAGCACAT+AGG 0.552956 1:-36133743 None:intergenic
GGACAGGTTGGCATCAAAGA+AGG 0.554332 1:-36135704 None:intergenic
TGTGTCGTCACCGTCGTCGT+CGG 0.554477 1:-36133384 None:intergenic
CAGAGGCTTTGGAATCAACA+TGG 0.562511 1:-36133601 None:intergenic
TAGCTGCTGTTGCACCTCCA+AGG 0.563262 1:+36135109 MsG0180002276.01.T01:three_prime_UTR
AATGCTACCAAGCGAATCAA+AGG 0.563600 1:-36135281 None:intergenic
TGCATTGGTTCCAGCTAGAG+TGG 0.566049 1:+36134785 MsG0180002276.01.T01:three_prime_UTR
GAAAGGGAGAGAAAATGTGA+AGG 0.566760 1:-36133261 None:intergenic
TTGATAATGAAAAGTGATTG+TGG 0.569754 1:-36133972 None:intergenic
CGCAAGGGTTCTTCAAGCCA+AGG 0.570759 1:+36133774 MsG0180002276.01.T01:CDS
TTTCTTGATTCAACAGGACG+AGG 0.572592 1:-36134373 None:intergenic
CAAGGAAACAGCTCTCATGT+AGG 0.574820 1:-36134310 None:intergenic
ATTCAGCAAAGACAAACAAA+TGG 0.576648 1:+36134169 MsG0180002276.01.T01:three_prime_UTR
AGTAACTAAGGTGGACAGGT+TGG 0.580001 1:-36135716 None:intergenic
AAATCAAGCTTCCACAGGGG+TGG 0.582687 1:+36135220 MsG0180002276.01.T01:three_prime_UTR
GGTAGCATTTAAGGGATTTG+GGG 0.591821 1:+36135295 MsG0180002276.01.T01:three_prime_UTR
ATAGGCTGAACGCAATAAGG+GGG 0.593373 1:+36134119 MsG0180002276.01.T01:three_prime_UTR
GATAGGCTGAACGCAATAAG+GGG 0.601595 1:+36134118 MsG0180002276.01.T01:three_prime_UTR
AAAGGGAGAGAAAATGTGAA+GGG 0.604477 1:-36133260 None:intergenic
GATGCAAGTGTTGTTGCGGC+TGG 0.606152 1:-36134851 None:intergenic
AAATGACAGCAAATGCCGCA+AGG 0.606582 1:+36133758 MsG0180002276.01.T01:CDS
CAGCAAAGACAAACAAATGG+AGG 0.610080 1:+36134172 MsG0180002276.01.T01:three_prime_UTR
TTGCTATGAATAATAATACA+AGG 0.610972 1:+36135738 MsG0180002276.01.T01:three_prime_UTR
AATGACAGCAAATGCCGCAA+GGG 0.612224 1:+36133759 MsG0180002276.01.T01:CDS
TGCTACCATAACCATTTCCA+AGG 0.612385 1:-36134328 None:intergenic
GGTCATTAGAAAGTCTCTCA+AGG 0.612790 1:+36135538 MsG0180002276.01.T01:three_prime_UTR
ATTACCGAAATGCGAAACAT+GGG 0.619209 1:-36133171 None:intergenic
TAGGCTGAACGCAATAAGGG+GGG 0.621088 1:+36134120 MsG0180002276.01.T01:three_prime_UTR
ATTGTTGTTATGATTCTCAG+AGG 0.625609 1:-36133618 None:intergenic
TTTGGAATCAACATGGAGAG+AGG 0.627829 1:-36133594 None:intergenic
GGGCTTGACCAGATTTGCAG+TGG 0.630621 1:+36135671 MsG0180002276.01.T01:three_prime_UTR
TTCATAGCAAGTAACTAAGG+TGG 0.641022 1:-36135725 None:intergenic
CAGTTTCTCTCCGACGACGA+CGG 0.644936 1:+36133374 MsG0180002276.01.T01:CDS
AAACGATGCAAGTGTTGTTG+CGG 0.650274 1:-36134855 None:intergenic
CATCAGTACGTCCACCCCTG+TGG 0.651769 1:-36135231 None:intergenic
AAAGCACATAGGGTTGATTG+TGG 0.656361 1:-36133732 None:intergenic
ATGGCTGAGAATTTGGATGA+CGG 0.669713 1:+36133332 MsG0180002276.01.T01:CDS
AGGCTGAACGCAATAAGGGG+GGG 0.672681 1:+36134121 MsG0180002276.01.T01:three_prime_UTR
AATGAAATCAAGCTTCCACA+GGG 0.674768 1:+36135216 MsG0180002276.01.T01:three_prime_UTR
GTTGCACCTCCAAGGCATAG+GGG 0.686212 1:+36135117 MsG0180002276.01.T01:three_prime_UTR
AGCTTGAAGACTATATAGTG+GGG 0.692039 1:+36134826 MsG0180002276.01.T01:three_prime_UTR
CTTGGCTTGAAGAACCCTTG+CGG 0.700347 1:-36133773 None:intergenic
GTATGCAGAAGCCAAAATGG+GGG 0.700850 1:+36134143 MsG0180002276.01.T01:three_prime_UTR
TCGTCGGAGAGAAACTGCGG+CGG 0.707718 1:-36133368 None:intergenic
TCGTCGTCGGAGAGAAACTG+CGG 0.728597 1:-36133371 None:intergenic
ATGAAATCAAGCTTCCACAG+GGG 0.800670 1:+36135217 MsG0180002276.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTTATTTATTTAATCAAAA+GGG + Chr1:36135017-36135036 MsG0180002276.01.T01:intron 10.0%
!! TAATAATAATAGAAGATAAA+TGG + Chr1:36135412-36135431 MsG0180002276.01.T01:three_prime_UTR 10.0%
!! TTTATTTATTTAATCAAAAG+GGG + Chr1:36135018-36135037 MsG0180002276.01.T01:intron 10.0%
!! AAATTATTAAATATCACAAC+CGG - Chr1:36134690-36134709 None:intergenic 15.0%
!! ACAATAAAAAAATTAAGAAG+GGG + Chr1:36135651-36135670 MsG0180002276.01.T01:three_prime_UTR 15.0%
!! AGACAATAAAAAAATTAAGA+AGG + Chr1:36135649-36135668 MsG0180002276.01.T01:three_prime_UTR 15.0%
!! GACAATAAAAAAATTAAGAA+GGG + Chr1:36135650-36135669 MsG0180002276.01.T01:three_prime_UTR 15.0%
!! GGGTAATAATAATAATAAAT+TGG + Chr1:36135618-36135637 MsG0180002276.01.T01:three_prime_UTR 15.0%
!! GGTTTATTTATTTAATCAAA+AGG + Chr1:36135016-36135035 MsG0180002276.01.T01:intron 15.0%
!! TTATTTATTTAATCAAAAGG+GGG + Chr1:36135019-36135038 MsG0180002276.01.T01:intron 15.0%
!! TTTAATAGAGAAAAGAAAAA+GGG - Chr1:36133217-36133236 None:intergenic 15.0%
!!! AAATCTCGTATTTTTAATAA+TGG + Chr1:36134028-36134047 MsG0180002276.01.T01:intron 15.0%
!!! AATAATAATAAATTGGTTTG+TGG + Chr1:36135625-36135644 MsG0180002276.01.T01:three_prime_UTR 15.0%
!!! AATCTCGTATTTTTAATAAT+GGG + Chr1:36134029-36134048 MsG0180002276.01.T01:intron 15.0%
!!! ATTTAAAATGTTACACAAAA+TGG + Chr1:36134550-36134569 MsG0180002276.01.T01:intron 15.0%
!!! ATTTTTTGTTTGGTTTTATT+AGG + Chr1:36135597-36135616 MsG0180002276.01.T01:three_prime_UTR 15.0%
!!! CAATTTGTATATTTTTTGTT+TGG + Chr1:36135587-36135606 MsG0180002276.01.T01:three_prime_UTR 15.0%
!!! GTTTTATATTTTTTCCTTTT+AGG + Chr1:36133664-36133683 MsG0180002276.01.T01:intron 15.0%
!!! TTTTAATAGAGAAAAGAAAA+AGG - Chr1:36133218-36133237 None:intergenic 15.0%
!!! TTTTTTACTATTACTATAGT+AGG + Chr1:36134995-36135014 MsG0180002276.01.T01:intron 15.0%
!!! TTTTTTGTTTGGTTTTATTA+GGG + Chr1:36135598-36135617 MsG0180002276.01.T01:three_prime_UTR 15.0%
!! ATGATGAAAATGATATGAAA+TGG + Chr1:36135257-36135276 MsG0180002276.01.T01:three_prime_UTR 20.0%
!! TATTTATTTAATCAAAAGGG+GGG + Chr1:36135020-36135039 MsG0180002276.01.T01:intron 20.0%
!! TTGCTATGAATAATAATACA+AGG + Chr1:36135738-36135757 MsG0180002276.01.T01:three_prime_UTR 20.0%
!!! AAAATCTAGTTTATAAGAGA+GGG - Chr1:36134973-36134992 None:intergenic 20.0%
!!! AAAGTTTTAATGCATTTAAG+AGG + Chr1:36134501-36134520 MsG0180002276.01.T01:intron 20.0%
!!! AAGTTTTAATGCATTTAAGA+GGG + Chr1:36134502-36134521 MsG0180002276.01.T01:intron 20.0%
!!! AATAGAAGATAAATGGTTTT+TGG + Chr1:36135419-36135438 MsG0180002276.01.T01:three_prime_UTR 20.0%
!!! AGTTTTATAGAAGAAACAAT+AGG + Chr1:36135354-36135373 MsG0180002276.01.T01:three_prime_UTR 20.0%
! AAATGGTTGAATCTAGTTAA+TGG + Chr1:36134567-36134586 MsG0180002276.01.T01:intron 25.0%
! AAGAGGCTAAAAATAATGTT+AGG + Chr1:36135447-36135466 MsG0180002276.01.T01:three_prime_UTR 25.0%
! ACAAAATTACATCATCATCA+TGG - Chr1:36134431-36134450 None:intergenic 25.0%
! ACCTAAATCATAACGAAAAA+CGG - Chr1:36134618-36134637 None:intergenic 25.0%
! AGAAATTCAACAAAAAAACC+AGG + Chr1:36134391-36134410 MsG0180002276.01.T01:three_prime_UTR 25.0%
! CAAAATTACATCATCATCAT+GGG - Chr1:36134430-36134449 None:intergenic 25.0%
! GAATCATAACAACAATAACT+TGG + Chr1:36133625-36133644 MsG0180002276.01.T01:CDS 25.0%
! TTATTCATAGCAAGTAACTA+AGG - Chr1:36135731-36135750 None:intergenic 25.0%
!! CAAAATCTAGTTTATAAGAG+AGG - Chr1:36134974-36134993 None:intergenic 25.0%
!! TACTTTTGTCTTTTTGATGA+GGG + Chr1:36135517-36135536 MsG0180002276.01.T01:three_prime_UTR 25.0%
!!! AACTTGGTATGCATTTTTTT+GGG + Chr1:36133641-36133660 MsG0180002276.01.T01:intron 25.0%
!!! ATGGATTATTTTTTTTGAGG+GGG + Chr1:36135046-36135065 MsG0180002276.01.T01:intron 25.0%
!!! CATGGATTATTTTTTTTGAG+GGG + Chr1:36135045-36135064 MsG0180002276.01.T01:intron 25.0%
!!! GCATGGATTATTTTTTTTGA+GGG + Chr1:36135044-36135063 MsG0180002276.01.T01:intron 25.0%
!!! TAACTTGGTATGCATTTTTT+TGG + Chr1:36133640-36133659 MsG0180002276.01.T01:intron 25.0%
!!! TGCATGGATTATTTTTTTTG+AGG + Chr1:36135043-36135062 MsG0180002276.01.T01:intron 25.0%
!!! TTACTTTTGTCTTTTTGATG+AGG + Chr1:36135516-36135535 MsG0180002276.01.T01:three_prime_UTR 25.0%
!!! TTGATAATGAAAAGTGATTG+TGG - Chr1:36133975-36133994 None:intergenic 25.0%
AAAGCTTGAAGACTATATAG+TGG + Chr1:36134824-36134843 MsG0180002276.01.T01:three_prime_UTR 30.0%
AAGCTTGAAGACTATATAGT+GGG + Chr1:36134825-36134844 MsG0180002276.01.T01:three_prime_UTR 30.0%
ACAAAAACAGAGGAAATTGA+AGG - Chr1:36133291-36133310 None:intergenic 30.0%
ACAAGCTACAATCACAAAAT+TGG - Chr1:36134760-36134779 None:intergenic 30.0%
ATTCAGCAAAGACAAACAAA+TGG + Chr1:36134169-36134188 MsG0180002276.01.T01:three_prime_UTR 30.0%
CAAAAACAGAGGAAATTGAA+GGG - Chr1:36133290-36133309 None:intergenic 30.0%
CCTAAATCATAACGAAAAAC+GGG - Chr1:36134617-36134636 None:intergenic 30.0%
CTATATAGTCTTCAAGCTTT+AGG - Chr1:36134824-36134843 None:intergenic 30.0%
GAAAAGAAAAAGGGATTATG+TGG - Chr1:36133208-36133227 None:intergenic 30.0%
GTTGAATTTCTTGATTCAAC+AGG - Chr1:36134382-36134401 None:intergenic 30.0%
TCAAAACGCAAAAACCTAAA+AGG - Chr1:36133681-36133700 None:intergenic 30.0%
! AGTACCATTTTCAACATTAG+AGG - Chr1:36134888-36134907 None:intergenic 30.0%
! ATTGTTGTTATGATTCTCAG+AGG - Chr1:36133621-36133640 None:intergenic 30.0%
! GAAGAAACAATAGGTCTTTT+TGG + Chr1:36135363-36135382 MsG0180002276.01.T01:three_prime_UTR 30.0%
! TTAATGTACGTTTTTGCTAC+TGG + Chr1:36134642-36134661 MsG0180002276.01.T01:intron 30.0%
!! GTTTTTGGAGGTAAGTAATA+GGG - Chr1:36133925-36133944 None:intergenic 30.0%
!! TTTTTGGAGGTAAGTAATAG+GGG - Chr1:36133924-36133943 None:intergenic 30.0%
!!! ACTTGGTATGCATTTTTTTG+GGG + Chr1:36133642-36133661 MsG0180002276.01.T01:intron 30.0%
!!! GTTTTTATTGTCGTTTTGCA+GGG + Chr1:36133702-36133721 MsG0180002276.01.T01:intron 30.0%
!!! TGTACGTTTTTTTGCGTTTT+TGG + Chr1:36134475-36134494 MsG0180002276.01.T01:intron 30.0%
!!! TGTTTTTGGAGGTAAGTAAT+AGG - Chr1:36133926-36133945 None:intergenic 30.0%
!!! TTGCTACTGGTTTAATGTTT+TGG + Chr1:36134655-36134674 MsG0180002276.01.T01:intron 30.0%
AAAGGGAGAGAAAATGTGAA+GGG - Chr1:36133263-36133282 None:intergenic 35.0%
AAATAATGTTAGGAGCCTCT+TGG + Chr1:36135457-36135476 MsG0180002276.01.T01:three_prime_UTR 35.0%
AAGAAAAAGGGATTATGTGG+AGG - Chr1:36133205-36133224 None:intergenic 35.0%
AATCCATAAGGAAAAAGCAG+AGG - Chr1:36133464-36133483 None:intergenic 35.0%
AATGAAATCAAGCTTCCACA+GGG + Chr1:36135216-36135235 MsG0180002276.01.T01:three_prime_UTR 35.0%
ACAGAGAGAAACAAAAACAG+AGG - Chr1:36133301-36133320 None:intergenic 35.0%
AGAAAAAGGGATTATGTGGA+GGG - Chr1:36133204-36133223 None:intergenic 35.0%
AGCTTGAAGACTATATAGTG+GGG + Chr1:36134826-36134845 MsG0180002276.01.T01:three_prime_UTR 35.0%
AGGAAATTGAAGGGAAAGAA+AGG - Chr1:36133281-36133300 None:intergenic 35.0%
AGGGGATTTGATGTAACAAA+AGG + Chr1:36135326-36135345 MsG0180002276.01.T01:three_prime_UTR 35.0%
ATGGGAAAAAAGTAATCACC+TGG - Chr1:36134412-36134431 None:intergenic 35.0%
ATTACCGAAATGCGAAACAT+GGG - Chr1:36133174-36133193 None:intergenic 35.0%
CAAAAAAGCTCAAATCAGCA+GGG - Chr1:36133950-36133969 None:intergenic 35.0%
CATTCTCACAAACACCATTA+GGG - Chr1:36133803-36133822 None:intergenic 35.0%
CCTCTTGGATTAAAAATCTC+AGG + Chr1:36135472-36135491 MsG0180002276.01.T01:three_prime_UTR 35.0%
CTCTCAAGGAAAGACAAAAA+TGG + Chr1:36135552-36135571 MsG0180002276.01.T01:three_prime_UTR 35.0%
GGAAATTGAAGGGAAAGAAA+GGG - Chr1:36133280-36133299 None:intergenic 35.0%
TAGGTTGTGGAGTTTGAATT+TGG - Chr1:36134294-36134313 None:intergenic 35.0%
TCAAGGAAAGACAAAAATGG+TGG + Chr1:36135555-36135574 MsG0180002276.01.T01:three_prime_UTR 35.0%
TCCACCTCTAATGTTGAAAA+TGG + Chr1:36134881-36134900 MsG0180002276.01.T01:three_prime_UTR 35.0%
TGGTAGCATTTAAGGGATTT+GGG + Chr1:36135294-36135313 MsG0180002276.01.T01:three_prime_UTR 35.0%
TGTTTCCTTGGAAATGGTTA+TGG + Chr1:36134323-36134342 MsG0180002276.01.T01:three_prime_UTR 35.0%
TTCATAGCAAGTAACTAAGG+TGG - Chr1:36135728-36135747 None:intergenic 35.0%
TTTCGCATTTCGGTAATCAT+GGG + Chr1:36133177-36133196 MsG0180002276.01.T01:five_prime_UTR 35.0%
! ACCATTTTCAACATTAGAGG+TGG - Chr1:36134885-36134904 None:intergenic 35.0%
! ATTCGCTTGGTAGCATTTAA+GGG + Chr1:36135287-36135306 MsG0180002276.01.T01:three_prime_UTR 35.0%
! ATTTTCCATGGCTGAGAATT+TGG + Chr1:36133325-36133344 MsG0180002276.01.T01:exon 35.0%
! CCTGAGATTTTTAATCCAAG+AGG - Chr1:36135475-36135494 None:intergenic 35.0%
! CTCTCTGTTTTCATTTTCCA+TGG + Chr1:36133313-36133332 MsG0180002276.01.T01:exon 35.0%
! GAGAAAATGTGAAGGGATTT+TGG - Chr1:36133256-36133275 None:intergenic 35.0%
! GTCATTTCCAAAAGCACATA+GGG - Chr1:36133745-36133764 None:intergenic 35.0%
! GTCTTTTTGGATGAAGATGT+AGG + Chr1:36135376-36135395 MsG0180002276.01.T01:three_prime_UTR 35.0%
! TGTCATTTCCAAAAGCACAT+AGG - Chr1:36133746-36133765 None:intergenic 35.0%
! TTGGTAGCATTTAAGGGATT+TGG + Chr1:36135293-36135312 MsG0180002276.01.T01:three_prime_UTR 35.0%
!! AGTCTTCAAGCTTTAGGTTA+AGG - Chr1:36134818-36134837 None:intergenic 35.0%
!! CCCGTTTTTCGTTATGATTT+AGG + Chr1:36134614-36134633 MsG0180002276.01.T01:intron 35.0%
!! GGTTTAATGTTTTGGATGTG+TGG + Chr1:36134663-36134682 MsG0180002276.01.T01:intron 35.0%
!! GTCTTCAAGCTTTAGGTTAA+GGG - Chr1:36134817-36134836 None:intergenic 35.0%
!!! AATGGTTTTTGGTTAGCAAG+AGG + Chr1:36135430-36135449 MsG0180002276.01.T01:three_prime_UTR 35.0%
!!! AATGTGAAGGGATTTTGGTT+AGG - Chr1:36133251-36133270 None:intergenic 35.0%
!!! CGTTTTTATTGTCGTTTTGC+AGG + Chr1:36133701-36133720 MsG0180002276.01.T01:intron 35.0%
!!! GATTTTAGTTGCAGAGGATT+AGG + Chr1:36134080-36134099 MsG0180002276.01.T01:intron 35.0%
!!! GGATTTTGGTTAGGTTTGTT+AGG - Chr1:36133242-36133261 None:intergenic 35.0%
!!! TGTGATGATTTTAGTTGCAG+AGG + Chr1:36134074-36134093 MsG0180002276.01.T01:intron 35.0%
AACAACAACAACAACGTTGC+TGG + Chr1:36134217-36134236 MsG0180002276.01.T01:three_prime_UTR 40.0%
AATGCTACCAAGCGAATCAA+AGG - Chr1:36135284-36135303 None:intergenic 40.0%
ACATTATTACCCCTATGCCT+TGG - Chr1:36135129-36135148 None:intergenic 40.0%
AGGAAAGACAAAAATGGTGG+TGG + Chr1:36135558-36135577 MsG0180002276.01.T01:three_prime_UTR 40.0%
ATGAAATCAAGCTTCCACAG+GGG + Chr1:36135217-36135236 MsG0180002276.01.T01:three_prime_UTR 40.0%
ATGGCTGAGAATTTGGATGA+CGG + Chr1:36133332-36133351 MsG0180002276.01.T01:CDS 40.0%
CAATGAAATCAAGCTTCCAC+AGG + Chr1:36135215-36135234 MsG0180002276.01.T01:three_prime_UTR 40.0%
CAGCAAAGACAAACAAATGG+AGG + Chr1:36134172-36134191 MsG0180002276.01.T01:three_prime_UTR 40.0%
CTGTAATACCACTGCAAATC+TGG - Chr1:36135682-36135701 None:intergenic 40.0%
GAAAGGGAGAGAAAATGTGA+AGG - Chr1:36133264-36133283 None:intergenic 40.0%
GAAGCAGCAAGAGAATCAAT+TGG - Chr1:36133509-36133528 None:intergenic 40.0%
GATTACCGAAATGCGAAACA+TGG - Chr1:36133175-36133194 None:intergenic 40.0%
GCAAAAAAGCTCAAATCAGC+AGG - Chr1:36133951-36133970 None:intergenic 40.0%
GCATTCTCACAAACACCATT+AGG - Chr1:36133804-36133823 None:intergenic 40.0%
GGAGGAAGAAATGTTGTTGA+TGG + Chr1:36134190-36134209 MsG0180002276.01.T01:three_prime_UTR 40.0%
GGCAACAACAAATTGCTGAT+AGG + Chr1:36134101-36134120 MsG0180002276.01.T01:three_prime_UTR 40.0%
GGGGAAGAAGAATTTCTTTG+AGG - Chr1:36133905-36133924 None:intergenic 40.0%
GGTAGCATTTAAGGGATTTG+GGG + Chr1:36135295-36135314 MsG0180002276.01.T01:three_prime_UTR 40.0%
GGTCATTAGAAAGTCTCTCA+AGG + Chr1:36135538-36135557 MsG0180002276.01.T01:three_prime_UTR 40.0%
GTTATGATTCTCAGAGGCTT+TGG - Chr1:36133615-36133634 None:intergenic 40.0%
GTTTCGCATTTCGGTAATCA+TGG + Chr1:36133176-36133195 MsG0180002276.01.T01:five_prime_UTR 40.0%
TAGGTTTGTCATCATCTGCA+TGG + Chr1:36134242-36134261 MsG0180002276.01.T01:three_prime_UTR 40.0%
TCAGAACAAGCGAATCCATA+AGG - Chr1:36133476-36133495 None:intergenic 40.0%
TGATAGGCTGAACGCAATAA+GGG + Chr1:36134117-36134136 MsG0180002276.01.T01:three_prime_UTR 40.0%
TGCTACCATAACCATTTCCA+AGG - Chr1:36134331-36134350 None:intergenic 40.0%
TGTTGAGCATGTTGTAGTGT+TGG - Chr1:36134268-36134287 None:intergenic 40.0%
TTGAAAAGCGAAAGCGAAGA+AGG - Chr1:36133428-36133447 None:intergenic 40.0%
TTTCTTGATTCAACAGGACG+AGG - Chr1:36134376-36134395 None:intergenic 40.0%
! AAACGATGCAAGTGTTGTTG+CGG - Chr1:36134858-36134877 None:intergenic 40.0%
! AAAGCACATAGGGTTGATTG+TGG - Chr1:36133735-36133754 None:intergenic 40.0%
! AAATGGTCCTTTGATTCGCT+TGG + Chr1:36135274-36135293 MsG0180002276.01.T01:three_prime_UTR 40.0%
! CAATCAACCCTATGTGCTTT+TGG + Chr1:36133735-36133754 MsG0180002276.01.T01:CDS 40.0%
! GATTCGCTTGGTAGCATTTA+AGG + Chr1:36135286-36135305 MsG0180002276.01.T01:three_prime_UTR 40.0%
! TTTGGAATCAACATGGAGAG+AGG - Chr1:36133597-36133616 None:intergenic 40.0%
!! AACAACAACGTTGCTGGTTT+AGG + Chr1:36134223-36134242 MsG0180002276.01.T01:three_prime_UTR 40.0%
!! AAGACAAAAATGGTGGTGGA+TGG + Chr1:36135562-36135581 MsG0180002276.01.T01:three_prime_UTR 40.0%
!! AATGTTTTGGATGTGTGGAC+CGG + Chr1:36134668-36134687 MsG0180002276.01.T01:intron 40.0%
!! AGACAAAAATGGTGGTGGAT+GGG + Chr1:36135563-36135582 MsG0180002276.01.T01:three_prime_UTR 40.0%
!! CAAATCAGCAGGGTTGTTTT+TGG - Chr1:36133940-36133959 None:intergenic 40.0%
!! TCGCTTTCGCTTTTCAACAA+TGG + Chr1:36133431-36133450 MsG0180002276.01.T01:CDS 40.0%
!!! AGGGATTTGGGGAGTATTTT+AGG + Chr1:36135306-36135325 MsG0180002276.01.T01:three_prime_UTR 40.0%
!!! GATCCTCTGCTTTTTCCTTA+TGG + Chr1:36133458-36133477 MsG0180002276.01.T01:CDS 40.0%
!!! GGATTTGGGGAGTATTTTAG+GGG + Chr1:36135308-36135327 MsG0180002276.01.T01:three_prime_UTR 40.0%
!!! GGGATTTGGGGAGTATTTTA+GGG + Chr1:36135307-36135326 MsG0180002276.01.T01:three_prime_UTR 40.0%
!!! TTTATTGTCGTTTTGCAGGG+AGG + Chr1:36133705-36133724 MsG0180002276.01.T01:intron 40.0%
AAACACCATTAGGGCTACCT+TGG - Chr1:36133794-36133813 None:intergenic 45.0%
AAATGACAGCAAATGCCGCA+AGG + Chr1:36133758-36133777 MsG0180002276.01.T01:CDS 45.0%
AACAGCTCTCATGTAGGTTG+TGG - Chr1:36134307-36134326 None:intergenic 45.0%
AATGACAGCAAATGCCGCAA+GGG + Chr1:36133759-36133778 MsG0180002276.01.T01:CDS 45.0%
AATGGTTATGGTAGCAGCAC+TGG + Chr1:36134335-36134354 MsG0180002276.01.T01:three_prime_UTR 45.0%
AGCAAGTAACTAAGGTGGAC+AGG - Chr1:36135723-36135742 None:intergenic 45.0%
AGCGAAAGCGAAGAAGGTTT+CGG - Chr1:36133422-36133441 None:intergenic 45.0%
AGTAACTAAGGTGGACAGGT+TGG - Chr1:36135719-36135738 None:intergenic 45.0%
ATAGGCTGAACGCAATAAGG+GGG + Chr1:36134119-36134138 MsG0180002276.01.T01:three_prime_UTR 45.0%
CAAGGAAACAGCTCTCATGT+AGG - Chr1:36134313-36134332 None:intergenic 45.0%
CTCTCCCATGTTTCGCATTT+CGG + Chr1:36133167-36133186 MsG0180002276.01.T01:five_prime_UTR 45.0%
CTGATAGGCTGAACGCAATA+AGG + Chr1:36134116-36134135 MsG0180002276.01.T01:three_prime_UTR 45.0%
GATAGGCTGAACGCAATAAG+GGG + Chr1:36134118-36134137 MsG0180002276.01.T01:three_prime_UTR 45.0%
GGGTATGCAGAAGCCAAAAT+GGG + Chr1:36134141-36134160 MsG0180002276.01.T01:three_prime_UTR 45.0%
GGTATGCAGAAGCCAAAATG+GGG + Chr1:36134142-36134161 MsG0180002276.01.T01:three_prime_UTR 45.0%
GTATGCAGAAGCCAAAATGG+GGG + Chr1:36134143-36134162 MsG0180002276.01.T01:three_prime_UTR 45.0%
GTCATCCAAATTCTCAGCCA+TGG - Chr1:36133333-36133352 None:intergenic 45.0%
TTATTACCCCTATGCCTTGG+AGG - Chr1:36135126-36135145 None:intergenic 45.0%
! ATTTGGATGACGGTGAGTTC+TGG + Chr1:36133342-36133361 MsG0180002276.01.T01:CDS 45.0%
! CGAAGAAGGTTTCGGTTTTG+TGG - Chr1:36133414-36133433 None:intergenic 45.0%
! CTACATGAGAGCTGTTTCCT+TGG + Chr1:36134311-36134330 MsG0180002276.01.T01:three_prime_UTR 45.0%
! GAGAGCTGTTTCCTTGGAAA+TGG + Chr1:36134317-36134336 MsG0180002276.01.T01:three_prime_UTR 45.0%
! TGTAGCTTGTGCAACTGCAT+TGG + Chr1:36134770-36134789 MsG0180002276.01.T01:intron 45.0%
! TTAATCAAAAGGGGGGTGCA+TGG + Chr1:36135027-36135046 MsG0180002276.01.T01:intron 45.0%
!! AGAAGGTTTCGGTTTTGTGG+TGG - Chr1:36133411-36133430 None:intergenic 45.0%
!! CAGAGGCTTTGGAATCAACA+TGG - Chr1:36133604-36133623 None:intergenic 45.0%
!!! ATCAGCAGGGTTGTTTTTGG+AGG - Chr1:36133937-36133956 None:intergenic 45.0%
AAATCAAGCTTCCACAGGGG+TGG + Chr1:36135220-36135239 MsG0180002276.01.T01:three_prime_UTR 50.0%
GGGGTATGCAGAAGCCAAAA+TGG + Chr1:36134140-36134159 MsG0180002276.01.T01:three_prime_UTR 50.0%
TAGGCTGAACGCAATAAGGG+GGG + Chr1:36134120-36134139 MsG0180002276.01.T01:three_prime_UTR 50.0%
TCAAGCCAAGGTAGCCCTAA+TGG + Chr1:36133786-36133805 MsG0180002276.01.T01:CDS 50.0%
TGTTGCACCTCCAAGGCATA+GGG + Chr1:36135116-36135135 MsG0180002276.01.T01:three_prime_UTR 50.0%
! CTTGGCTTGAAGAACCCTTG+CGG - Chr1:36133776-36133795 None:intergenic 50.0%
! GGACAGGTTGGCATCAAAGA+AGG - Chr1:36135707-36135726 None:intergenic 50.0%
! TGCTGAATGCACCCCCATTT+TGG - Chr1:36134157-36134176 None:intergenic 50.0%
!! TGCATTGGTTCCAGCTAGAG+TGG + Chr1:36134785-36134804 MsG0180002276.01.T01:three_prime_UTR 50.0%
AGGCTGAACGCAATAAGGGG+GGG + Chr1:36134121-36134140 MsG0180002276.01.T01:three_prime_UTR 55.0%
CAGTTTCTCTCCGACGACGA+CGG + Chr1:36133374-36133393 MsG0180002276.01.T01:CDS 55.0%
CGCAAGGGTTCTTCAAGCCA+AGG + Chr1:36133774-36133793 MsG0180002276.01.T01:CDS 55.0%
CTGTTGCACCTCCAAGGCAT+AGG + Chr1:36135115-36135134 MsG0180002276.01.T01:three_prime_UTR 55.0%
GCAGCAGCAACAATACCAGC+AGG + Chr1:36135166-36135185 MsG0180002276.01.T01:three_prime_UTR 55.0%
GGGCTTGACCAGATTTGCAG+TGG + Chr1:36135671-36135690 MsG0180002276.01.T01:three_prime_UTR 55.0%
GTTGCACCTCCAAGGCATAG+GGG + Chr1:36135117-36135136 MsG0180002276.01.T01:three_prime_UTR 55.0%
TAGCTGCTGTTGCACCTCCA+AGG + Chr1:36135109-36135128 MsG0180002276.01.T01:three_prime_UTR 55.0%
TCGTCGTCGGAGAGAAACTG+CGG - Chr1:36133374-36133393 None:intergenic 55.0%
! GATGCAAGTGTTGTTGCGGC+TGG - Chr1:36134854-36134873 None:intergenic 55.0%
!! ATGGTAGCAGCACTGGCACT+GGG + Chr1:36134342-36134361 MsG0180002276.01.T01:three_prime_UTR 55.0%
!! TATGGTAGCAGCACTGGCAC+TGG + Chr1:36134341-36134360 MsG0180002276.01.T01:three_prime_UTR 55.0%
CATCAGTACGTCCACCCCTG+TGG - Chr1:36135234-36135253 None:intergenic 60.0%
TCGTCGGAGAGAAACTGCGG+CGG - Chr1:36133371-36133390 None:intergenic 60.0%
TGTGTCGTCACCGTCGTCGT+CGG - Chr1:36133387-36133406 None:intergenic 60.0%
TTGCTCACTGCTGCTGCTGC+TGG - Chr1:36135198-36135217 None:intergenic 60.0%
AGGGCATGCGCCACTCTAGC+TGG - Chr1:36134798-36134817 None:intergenic 65.0%
! ACTGCTGCTGCTGCTGGCTG+AGG - Chr1:36135192-36135211 None:intergenic 65.0%
! TGCTGCTGGCTGAGGCCTGC+TGG - Chr1:36135184-36135203 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 36133117 36135769 36133117 ID=MsG0180002276.01;Name=MsG0180002276.01
Chr1 mRNA 36133117 36135769 36133117 ID=MsG0180002276.01.T01;Parent=MsG0180002276.01;Name=MsG0180002276.01.T01;_AED=0.06;_eAED=0.06;_QI=215|1|1|1|0.25|0.4|5|1281|144
Chr1 exon 36133117 36133646 36133117 ID=MsG0180002276.01.T01:exon:1120;Parent=MsG0180002276.01.T01
Chr1 exon 36133723 36134009 36133723 ID=MsG0180002276.01.T01:exon:1121;Parent=MsG0180002276.01.T01
Chr1 exon 36134088 36134412 36134088 ID=MsG0180002276.01.T01:exon:1122;Parent=MsG0180002276.01.T01
Chr1 exon 36134775 36134902 36134775 ID=MsG0180002276.01.T01:exon:1123;Parent=MsG0180002276.01.T01
Chr1 exon 36135109 36135769 36135109 ID=MsG0180002276.01.T01:exon:1124;Parent=MsG0180002276.01.T01
Chr1 five_prime_UTR 36133117 36133331 36133117 ID=MsG0180002276.01.T01:five_prime_utr;Parent=MsG0180002276.01.T01
Chr1 CDS 36133332 36133646 36133332 ID=MsG0180002276.01.T01:cds;Parent=MsG0180002276.01.T01
Chr1 CDS 36133723 36133842 36133723 ID=MsG0180002276.01.T01:cds;Parent=MsG0180002276.01.T01
Chr1 three_prime_UTR 36133843 36134009 36133843 ID=MsG0180002276.01.T01:three_prime_utr;Parent=MsG0180002276.01.T01
Chr1 three_prime_UTR 36134088 36134412 36134088 ID=MsG0180002276.01.T01:three_prime_utr;Parent=MsG0180002276.01.T01
Chr1 three_prime_UTR 36134775 36134902 36134775 ID=MsG0180002276.01.T01:three_prime_utr;Parent=MsG0180002276.01.T01
Chr1 three_prime_UTR 36135109 36135769 36135109 ID=MsG0180002276.01.T01:three_prime_utr;Parent=MsG0180002276.01.T01
Gene Sequence

>MsG0180002276.01.T01

ATGGCTGAGAATTTGGATGACGGTGAGTTCTGGCTTCCGCCGCAGTTTCTCTCCGACGACGACGGTGACGACACAACCACCACAAAACCGAAACCTTCTTCGCTTTCGCTTTTCAACAATGGTGAAGATCCTCTGCTTTTTCCTTATGGATTCGCTTGTTCTGATCTCAGTTCACCAATTGATTCTCTTGCTGCTTCAAGCGAAACTGAAAGCGATGATGATGAACAACTCTTCGCTGAGTTAACTCGTTGCTTGTCTCGTTCCTCTCTCCATGTTGATTCCAAAGCCTCTGAGAATCATAACAACAATAACTTGGGAGGTTCACCACAATCAACCCTATGTGCTTTTGGAAATGACAGCAAATGCCGCAAGGGTTCTTCAAGCCAAGGTAGCCCTAATGGTGTTTGTGAGAATGCTTCTTCAACTCTACTTTGA
Protein sequence

>MsG0180002276.01.T01

MAENLDDGEFWLPPQFLSDDDGDDTTTTKPKPSSLSLFNNGEDPLLFPYGFACSDLSSPIDSLAASSETESDDDEQLFAELTRCLSRSSLHVDSKASENHNNNNLGGSPQSTLCAFGNDSKCRKGSSSQGSPNGVCENASSTLL*