Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046944.01.T01 | RHN60225.1 | 80 | 135 | 26 | 1 | 1 | 134 | 1 | 135 | 1.02E-70 | 223 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046944.01.T01 | Q43077 | 70.079 | 127 | 37 | 1 | 1 | 126 | 1 | 127 | 4.22E-54 | 182 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046944.01.T01 | A0A396I997 | 80.000 | 135 | 26 | 1 | 1 | 134 | 1 | 135 | 4.87e-71 | 223 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880045585.01 | MsG0880046944.01 | 0.821526 | 3.852845e-53 | 3.142077e-50 |
| MsG0880045586.01 | MsG0880046944.01 | 0.817175 | 3.779364e-52 | 2.732553e-49 |
| MsG0880046942.01 | MsG0880046944.01 | 0.855403 | 6.457373e-62 | 1.489232e-58 |
| MsG0680033143.01 | MsG0880046944.01 | 0.818574 | 1.826240e-52 | 1.372090e-49 |
| MsG0380016491.01 | MsG0880046944.01 | 0.806884 | 6.621734e-50 | 3.635557e-47 |
| MsG0380017869.01 | MsG0880046944.01 | 0.809897 | 1.507364e-50 | 8.958247e-48 |
| MsG0580029405.01 | MsG0880046944.01 | 0.844048 | 9.620654e-59 | 1.534520e-55 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046944.01.T01 | MTR_4g045887 | 84.127 | 126 | 20 | 0 | 1 | 126 | 1 | 126 | 2.12e-70 | 223 |
| MsG0880046944.01.T01 | MTR_4g117610 | 90.083 | 121 | 11 | 1 | 7 | 126 | 1 | 121 | 5.07e-68 | 217 |
| MsG0880046944.01.T01 | MTR_4g117660 | 71.875 | 128 | 32 | 3 | 1 | 125 | 1 | 127 | 1.17e-53 | 179 |
| MsG0880046944.01.T01 | MTR_4g098910 | 63.235 | 68 | 23 | 2 | 60 | 125 | 22 | 89 | 9.21e-21 | 82.4 |
| MsG0880046944.01.T01 | MTR_1g104590 | 37.500 | 128 | 67 | 5 | 7 | 126 | 3 | 125 | 1.50e-15 | 72.4 |
| MsG0880046944.01.T01 | MTR_1g104550 | 31.959 | 97 | 63 | 1 | 32 | 125 | 34 | 130 | 3.12e-13 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046944.01.T01 | AT1G31710 | 37.624 | 101 | 56 | 3 | 31 | 126 | 27 | 125 | 1.11e-18 | 81.3 |
| MsG0880046944.01.T01 | AT1G31670 | 36.735 | 98 | 59 | 2 | 32 | 126 | 107 | 204 | 8.21e-16 | 73.2 |
| MsG0880046944.01.T01 | AT1G31690 | 37.374 | 99 | 57 | 3 | 32 | 126 | 27 | 124 | 1.36e-15 | 72.4 |
| MsG0880046944.01.T01 | AT4G14940 | 35.345 | 116 | 72 | 1 | 13 | 125 | 4 | 119 | 1.28e-13 | 67.0 |
| MsG0880046944.01.T01 | AT1G62810 | 34.021 | 97 | 61 | 2 | 31 | 126 | 59 | 153 | 4.00e-13 | 65.5 |
| MsG0880046944.01.T01 | AT3G43670 | 34.343 | 99 | 58 | 3 | 31 | 126 | 40 | 134 | 4.31e-13 | 65.5 |
Find 35 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAACTCTTGTTTGGTTAA+AGG | 0.191085 | 8:+80239959 | None:intergenic |
| GACAATGTTCATAAGGGATA+TGG | 0.263734 | 8:-80239719 | MsG0880046944.01.T01:CDS |
| GAAACTCTTGTTTGGTTAAA+GGG | 0.311803 | 8:+80239960 | None:intergenic |
| TTGAGGTGGGATATTTGTTA+TGG | 0.322352 | 8:+80239918 | None:intergenic |
| ATTTGTTATGGACTAGGGTT+TGG | 0.352768 | 8:+80239930 | None:intergenic |
| TGGGATATTTGTTATGGACT+AGG | 0.372057 | 8:+80239924 | None:intergenic |
| ATGAGATAGTAATTGATATA+AGG | 0.372118 | 8:-80239760 | MsG0880046944.01.T01:CDS |
| AGGGTAGCTTTCCACTATAT+TGG | 0.387861 | 8:-80239890 | MsG0880046944.01.T01:CDS |
| TGTCTCTGACAATGTTCATA+AGG | 0.400670 | 8:-80239726 | MsG0880046944.01.T01:CDS |
| GACAGTTTGAAACTCTTGTT+TGG | 0.403538 | 8:+80239952 | None:intergenic |
| TTGAAGATATAATCTTTGTC+GGG | 0.403769 | 8:+80239857 | None:intergenic |
| AATATCCCACCTCAAAGAAC+AGG | 0.415115 | 8:-80239910 | MsG0880046944.01.T01:CDS |
| GTCTCTGACAATGTTCATAA+GGG | 0.439897 | 8:-80239725 | MsG0880046944.01.T01:CDS |
| TTTGTCGTTGCTATCATCAA+TGG | 0.440663 | 8:-80239791 | MsG0880046944.01.T01:CDS |
| TTTGAAGATATAATCTTTGT+CGG | 0.446983 | 8:+80239856 | None:intergenic |
| GGGATATTTGTTATGGACTA+GGG | 0.447268 | 8:+80239925 | None:intergenic |
| GAAAGCTACCCTGTTCTTTG+AGG | 0.448413 | 8:+80239901 | None:intergenic |
| AATCTTTGTCGGGGTCATCT+AGG | 0.454398 | 8:+80239867 | None:intergenic |
| AGAGAAAAGAGCAAGTTTCA+TGG | 0.458128 | 8:+80240048 | None:intergenic |
| AGCAAGTTTCATGGTGGTGG+TGG | 0.466982 | 8:+80240057 | None:intergenic |
| AGCTACCCTGTTCTTTGAGG+TGG | 0.474719 | 8:+80239904 | None:intergenic |
| GATTTACGACGGATTACTAC+AGG | 0.535869 | 8:+80239815 | None:intergenic |
| GGTCATCTAGGCCAATATAG+TGG | 0.536518 | 8:+80239879 | None:intergenic |
| AAGAGCAAGTTTCATGGTGG+TGG | 0.539834 | 8:+80240054 | None:intergenic |
| GCTTTACAGAGACCACTGCT+TGG | 0.563172 | 8:+80240005 | None:intergenic |
| GCTACCCTGTTCTTTGAGGT+GGG | 0.564691 | 8:+80239905 | None:intergenic |
| TGTTTGGTTAAAGGGTCAAG+TGG | 0.569696 | 8:+80239968 | None:intergenic |
| AGACCACTGCTTGGAAGTAG+AGG | 0.572715 | 8:+80240014 | None:intergenic |
| TCAAGTGGATGTTGACGCAA+TGG | 0.599243 | 8:+80239983 | None:intergenic |
| TGAAGATATAATCTTTGTCG+GGG | 0.624760 | 8:+80239858 | None:intergenic |
| ATTGATGATAGCAACGACAA+AGG | 0.669383 | 8:+80239793 | None:intergenic |
| AATCCTCTACTTCCAAGCAG+TGG | 0.680607 | 8:-80240017 | MsG0880046944.01.T01:CDS |
| ATATCCCACCTCAAAGAACA+GGG | 0.680963 | 8:-80239909 | MsG0880046944.01.T01:CDS |
| CAACGACAAAGGATTTACGA+CGG | 0.708733 | 8:+80239804 | None:intergenic |
| GAAAAGAGCAAGTTTCATGG+TGG | 0.732765 | 8:+80240051 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAGATAGTAATTGATATA+AGG | - | Chr8:80239986-80240005 | MsG0880046944.01.T01:CDS | 20.0% |
| !! | TTTGAAGATATAATCTTTGT+CGG | + | Chr8:80239893-80239912 | None:intergenic | 20.0% |
| ! | TTGAAGATATAATCTTTGTC+GGG | + | Chr8:80239892-80239911 | None:intergenic | 25.0% |
| CTATCCACTACTACAAAAAA+GGG | - | Chr8:80240055-80240074 | MsG0880046944.01.T01:CDS | 30.0% | |
| TGAAGATATAATCTTTGTCG+GGG | + | Chr8:80239891-80239910 | None:intergenic | 30.0% | |
| ! | GAAACTCTTGTTTGGTTAAA+GGG | + | Chr8:80239789-80239808 | None:intergenic | 30.0% |
| ! | TGAAACTCTTGTTTGGTTAA+AGG | + | Chr8:80239790-80239809 | None:intergenic | 30.0% |
| !!! | CTTTTTTGTAGTAGTGGATA+GGG | + | Chr8:80240056-80240075 | None:intergenic | 30.0% |
| AGAGAAAAGAGCAAGTTTCA+TGG | + | Chr8:80239701-80239720 | None:intergenic | 35.0% | |
| CCTATCCACTACTACAAAAA+AGG | - | Chr8:80240054-80240073 | MsG0880046944.01.T01:CDS | 35.0% | |
| GACAATGTTCATAAGGGATA+TGG | - | Chr8:80240027-80240046 | MsG0880046944.01.T01:CDS | 35.0% | |
| GTCTCTGACAATGTTCATAA+GGG | - | Chr8:80240021-80240040 | MsG0880046944.01.T01:CDS | 35.0% | |
| TGTCTCTGACAATGTTCATA+AGG | - | Chr8:80240020-80240039 | MsG0880046944.01.T01:CDS | 35.0% | |
| TTTGTCGTTGCTATCATCAA+TGG | - | Chr8:80239955-80239974 | MsG0880046944.01.T01:CDS | 35.0% | |
| ! | ATTGATGATAGCAACGACAA+AGG | + | Chr8:80239956-80239975 | None:intergenic | 35.0% |
| ! | GACAGTTTGAAACTCTTGTT+TGG | + | Chr8:80239797-80239816 | None:intergenic | 35.0% |
| ! | TTGAGGTGGGATATTTGTTA+TGG | + | Chr8:80239831-80239850 | None:intergenic | 35.0% |
| !! | ATTTGTTATGGACTAGGGTT+TGG | + | Chr8:80239819-80239838 | None:intergenic | 35.0% |
| !! | GGGATATTTGTTATGGACTA+GGG | + | Chr8:80239824-80239843 | None:intergenic | 35.0% |
| !! | TGGGATATTTGTTATGGACT+AGG | + | Chr8:80239825-80239844 | None:intergenic | 35.0% |
| !!! | CCTTTTTTGTAGTAGTGGAT+AGG | + | Chr8:80240057-80240076 | None:intergenic | 35.0% |
| !!! | TAAGCCCTTTTTTGTAGTAG+TGG | + | Chr8:80240062-80240081 | None:intergenic | 35.0% |
| AATATCCCACCTCAAAGAAC+AGG | - | Chr8:80239836-80239855 | MsG0880046944.01.T01:CDS | 40.0% | |
| AGGGTAGCTTTCCACTATAT+TGG | - | Chr8:80239856-80239875 | MsG0880046944.01.T01:CDS | 40.0% | |
| ATATCCCACCTCAAAGAACA+GGG | - | Chr8:80239837-80239856 | MsG0880046944.01.T01:CDS | 40.0% | |
| CAACGACAAAGGATTTACGA+CGG | + | Chr8:80239945-80239964 | None:intergenic | 40.0% | |
| GAAAAGAGCAAGTTTCATGG+TGG | + | Chr8:80239698-80239717 | None:intergenic | 40.0% | |
| TGTTTGGTTAAAGGGTCAAG+TGG | + | Chr8:80239781-80239800 | None:intergenic | 40.0% | |
| !! | GATTTACGACGGATTACTAC+AGG | + | Chr8:80239934-80239953 | None:intergenic | 40.0% |
| AAGAGCAAGTTTCATGGTGG+TGG | + | Chr8:80239695-80239714 | None:intergenic | 45.0% | |
| AATCCTCTACTTCCAAGCAG+TGG | - | Chr8:80239729-80239748 | MsG0880046944.01.T01:CDS | 45.0% | |
| AATCTTTGTCGGGGTCATCT+AGG | + | Chr8:80239882-80239901 | None:intergenic | 45.0% | |
| GAAAGCTACCCTGTTCTTTG+AGG | + | Chr8:80239848-80239867 | None:intergenic | 45.0% | |
| GGTCATCTAGGCCAATATAG+TGG | + | Chr8:80239870-80239889 | None:intergenic | 45.0% | |
| TCAAGTGGATGTTGACGCAA+TGG | + | Chr8:80239766-80239785 | None:intergenic | 45.0% | |
| AGACCACTGCTTGGAAGTAG+AGG | + | Chr8:80239735-80239754 | None:intergenic | 50.0% | |
| GCTTTACAGAGACCACTGCT+TGG | + | Chr8:80239744-80239763 | None:intergenic | 50.0% | |
| ! | AGCTACCCTGTTCTTTGAGG+TGG | + | Chr8:80239845-80239864 | None:intergenic | 50.0% |
| !! | AGCAAGTTTCATGGTGGTGG+TGG | + | Chr8:80239692-80239711 | None:intergenic | 50.0% |
| !! | GCTACCCTGTTCTTTGAGGT+GGG | + | Chr8:80239844-80239863 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 80239682 | 80240086 | 80239682 | ID=MsG0880046944.01;Name=MsG0880046944.01 |
| Chr8 | mRNA | 80239682 | 80240086 | 80239682 | ID=MsG0880046944.01.T01;Parent=MsG0880046944.01;Name=MsG0880046944.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|134 |
| Chr8 | exon | 80239682 | 80240086 | 80239682 | ID=MsG0880046944.01.T01:exon:10293;Parent=MsG0880046944.01.T01 |
| Chr8 | CDS | 80239682 | 80240086 | 80239682 | ID=MsG0880046944.01.T01:cds;Parent=MsG0880046944.01.T01 |
| Gene Sequence |
| Protein sequence |
