Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047006.01.T01 | XP_003609600.1 | 97.531 | 81 | 2 | 0 | 1 | 81 | 1 | 81 | 9.22E-51 | 164 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047006.01.T01 | Q8L8Z8 | 82.716 | 81 | 13 | 1 | 1 | 81 | 1 | 80 | 1.24E-44 | 142 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047006.01.T01 | G7JJ49 | 97.531 | 81 | 2 | 0 | 1 | 81 | 1 | 81 | 4.40e-51 | 164 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880047004.01 | MsG0880047006.01 | 0.874185 | 8.227811e-68 | 3.668898e-64 |
| MsG0880047005.01 | MsG0880047006.01 | 0.884966 | 1.220620e-71 | 8.224582e-68 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047006.01.T01 | MTR_4g119050 | 97.531 | 81 | 2 | 0 | 1 | 81 | 1 | 81 | 1.12e-54 | 164 |
| MsG0880047006.01.T01 | MTR_4g119030 | 97.531 | 81 | 2 | 0 | 1 | 81 | 1 | 81 | 1.12e-54 | 164 |
| MsG0880047006.01.T01 | MTR_4g119040 | 97.531 | 81 | 2 | 0 | 1 | 81 | 1 | 81 | 1.12e-54 | 164 |
| MsG0880047006.01.T01 | MTR_2g019900 | 80.247 | 81 | 15 | 1 | 1 | 81 | 1 | 80 | 1.56e-42 | 134 |
| MsG0880047006.01.T01 | MTR_2g019950 | 66.250 | 80 | 26 | 1 | 1 | 80 | 1 | 79 | 5.08e-34 | 112 |
| MsG0880047006.01.T01 | MTR_1g088905 | 59.259 | 81 | 32 | 1 | 1 | 81 | 1 | 80 | 7.81e-30 | 102 |
| MsG0880047006.01.T01 | MTR_7g108250 | 55.556 | 81 | 35 | 1 | 1 | 81 | 1 | 80 | 2.83e-28 | 98.2 |
| MsG0880047006.01.T01 | MTR_7g026770 | 58.025 | 81 | 34 | 0 | 1 | 81 | 22 | 102 | 3.68e-28 | 102 |
| MsG0880047006.01.T01 | MTR_7g108220 | 53.086 | 81 | 38 | 0 | 1 | 81 | 1 | 81 | 1.61e-27 | 96.3 |
| MsG0880047006.01.T01 | MTR_7g022710 | 51.852 | 81 | 39 | 0 | 1 | 81 | 30 | 110 | 2.83e-27 | 96.3 |
| MsG0880047006.01.T01 | MTR_7g022690 | 51.852 | 81 | 39 | 0 | 1 | 81 | 30 | 110 | 7.98e-27 | 95.1 |
| MsG0880047006.01.T01 | MTR_7g108210 | 51.852 | 81 | 39 | 0 | 1 | 81 | 1 | 81 | 9.57e-27 | 94.4 |
| MsG0880047006.01.T01 | MTR_7g108200 | 49.383 | 81 | 40 | 1 | 1 | 81 | 1 | 80 | 1.57e-26 | 93.6 |
| MsG0880047006.01.T01 | MTR_1g088910 | 54.321 | 81 | 36 | 1 | 1 | 81 | 1 | 80 | 2.49e-26 | 93.2 |
| MsG0880047006.01.T01 | MTR_7g022550 | 50.617 | 81 | 40 | 0 | 1 | 81 | 30 | 110 | 6.00e-26 | 93.2 |
| MsG0880047006.01.T01 | MTR_1g088895 | 53.086 | 81 | 37 | 1 | 1 | 81 | 1 | 80 | 1.75e-25 | 91.3 |
| MsG0880047006.01.T01 | MTR_1g088920 | 49.383 | 81 | 40 | 1 | 1 | 81 | 1 | 80 | 4.80e-25 | 89.7 |
| MsG0880047006.01.T01 | MTR_1g088925 | 46.914 | 81 | 42 | 1 | 1 | 81 | 1 | 80 | 7.52e-25 | 89.4 |
| MsG0880047006.01.T01 | MTR_7g108260 | 46.914 | 81 | 42 | 1 | 1 | 81 | 1 | 80 | 1.04e-22 | 84.0 |
| MsG0880047006.01.T01 | MTR_1g069255 | 43.590 | 78 | 42 | 2 | 2 | 78 | 84 | 160 | 5.92e-15 | 66.2 |
| MsG0880047006.01.T01 | MTR_2g090755 | 39.744 | 78 | 47 | 0 | 4 | 81 | 41 | 118 | 6.35e-15 | 65.1 |
| MsG0880047006.01.T01 | MTR_2g014760 | 41.250 | 80 | 38 | 2 | 3 | 78 | 28 | 102 | 2.55e-14 | 63.2 |
| MsG0880047006.01.T01 | MTR_4g079110 | 41.975 | 81 | 37 | 3 | 6 | 81 | 58 | 133 | 5.08e-14 | 63.2 |
| MsG0880047006.01.T01 | MTR_1g015890 | 43.210 | 81 | 37 | 2 | 3 | 78 | 48 | 124 | 5.48e-14 | 62.8 |
| MsG0880047006.01.T01 | MTR_2g048970 | 45.000 | 80 | 41 | 1 | 2 | 78 | 29 | 108 | 1.88e-13 | 61.2 |
| MsG0880047006.01.T01 | MTR_7g035245 | 40.244 | 82 | 48 | 1 | 1 | 82 | 3 | 83 | 3.11e-13 | 60.1 |
| MsG0880047006.01.T01 | MTR_3g104510 | 37.662 | 77 | 41 | 2 | 3 | 78 | 23 | 93 | 3.97e-11 | 54.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047006.01.T01 | AT5G18600 | 82.716 | 81 | 13 | 1 | 1 | 81 | 1 | 80 | 1.26e-45 | 142 |
| MsG0880047006.01.T01 | AT4G15680 | 75.309 | 81 | 19 | 1 | 1 | 81 | 1 | 80 | 9.21e-43 | 134 |
| MsG0880047006.01.T01 | AT4G15690 | 76.543 | 81 | 18 | 1 | 1 | 81 | 1 | 80 | 3.29e-42 | 133 |
| MsG0880047006.01.T01 | AT4G15670 | 74.074 | 81 | 20 | 1 | 1 | 81 | 1 | 80 | 8.35e-42 | 132 |
| MsG0880047006.01.T01 | AT4G15700 | 74.074 | 81 | 20 | 1 | 1 | 81 | 1 | 80 | 1.46e-41 | 131 |
| MsG0880047006.01.T01 | AT4G15660 | 72.840 | 81 | 21 | 1 | 1 | 81 | 1 | 80 | 6.71e-41 | 130 |
| MsG0880047006.01.T01 | AT1G03020 | 60.494 | 81 | 31 | 1 | 1 | 81 | 1 | 80 | 4.32e-33 | 110 |
| MsG0880047006.01.T01 | AT3G62930 | 59.259 | 81 | 32 | 1 | 1 | 81 | 1 | 80 | 1.11e-31 | 106 |
| MsG0880047006.01.T01 | AT3G21460 | 60.000 | 80 | 31 | 1 | 1 | 80 | 1 | 79 | 2.25e-30 | 103 |
| MsG0880047006.01.T01 | AT1G06830 | 56.790 | 81 | 34 | 1 | 1 | 81 | 1 | 80 | 5.22e-29 | 99.8 |
| MsG0880047006.01.T01 | AT2G47870 | 54.321 | 81 | 37 | 0 | 1 | 81 | 1 | 81 | 5.60e-29 | 99.8 |
| MsG0880047006.01.T01 | AT2G30540 | 55.556 | 81 | 35 | 1 | 1 | 81 | 1 | 80 | 1.13e-28 | 99.0 |
| MsG0880047006.01.T01 | AT3G62950 | 49.383 | 81 | 41 | 0 | 1 | 81 | 1 | 81 | 1.39e-26 | 94.0 |
| MsG0880047006.01.T01 | AT2G47880 | 51.852 | 81 | 38 | 1 | 1 | 81 | 1 | 80 | 7.63e-26 | 92.0 |
| MsG0880047006.01.T01 | AT3G62960 | 49.383 | 81 | 40 | 1 | 1 | 81 | 1 | 80 | 2.56e-24 | 88.2 |
| MsG0880047006.01.T01 | AT5G14070 | 48.810 | 84 | 36 | 1 | 1 | 77 | 31 | 114 | 2.91e-22 | 84.0 |
| MsG0880047006.01.T01 | AT3G02000 | 50.588 | 85 | 35 | 2 | 3 | 81 | 31 | 114 | 7.06e-18 | 72.8 |
| MsG0880047006.01.T01 | AT1G28480 | 34.940 | 83 | 51 | 1 | 2 | 81 | 33 | 115 | 3.66e-13 | 60.5 |
| MsG0880047006.01.T01 | AT2G20270 | 38.961 | 77 | 47 | 0 | 2 | 78 | 76 | 152 | 5.66e-13 | 60.8 |
| MsG0880047006.01.T01 | AT4G33040 | 43.750 | 80 | 40 | 2 | 3 | 78 | 41 | 119 | 6.20e-13 | 60.1 |
| MsG0880047006.01.T01 | AT4G28730 | 42.308 | 78 | 43 | 2 | 2 | 78 | 71 | 147 | 8.69e-13 | 60.5 |
| MsG0880047006.01.T01 | AT5G63030 | 37.975 | 79 | 48 | 1 | 3 | 81 | 21 | 98 | 1.20e-11 | 56.2 |
| MsG0880047006.01.T01 | AT5G11930 | 40.260 | 77 | 35 | 3 | 3 | 78 | 57 | 123 | 3.15e-11 | 55.8 |
Find 12 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTCAATGTCTCTTCCTCT+TGG | 0.387857 | 8:-80869656 | None:intergenic |
| CAAGCACTTATTTCAAGACT+TGG | 0.435000 | 8:+80869677 | MsG0880047006.01.T01:CDS |
| TTCATTGGTGGTGAACTTGT+TGG | 0.438172 | 8:+80869725 | MsG0880047006.01.T01:CDS |
| GATGAAGACTCATGACTGAT+TGG | 0.460385 | 8:-80869753 | None:intergenic |
| TCATCAAGTTCATAAACTGC+TGG | 0.480601 | 8:-80869629 | None:intergenic |
| ATTGGTGGTGAACTTGTTGG+TGG | 0.517599 | 8:+80869728 | MsG0880047006.01.T01:CDS |
| ATGAAAACAGTTGGAACAGA+TGG | 0.519711 | 8:-80869707 | None:intergenic |
| TCACCACCAATGAAAACAGT+TGG | 0.567868 | 8:-80869716 | None:intergenic |
| CTCTTGCTGAATATCACCAC+TGG | 0.581366 | 8:-80869551 | None:intergenic |
| AATGGAGAGAGTAACAAAGA+TGG | 0.583009 | 8:+80869517 | None:intergenic |
| GAACTTGATGAGATACCAAG+AGG | 0.660515 | 8:+80869641 | MsG0880047006.01.T01:CDS |
| GATGGTTTCAGAGAGACCAG+TGG | 0.745891 | 8:+80869535 | MsG0880047006.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGACACTATTTTGTGACTT+TGG | + | Chr8:80869599-80869618 | MsG0880047006.01.T01:CDS | 30.0% |
| ATGAAAACAGTTGGAACAGA+TGG | - | Chr8:80869710-80869729 | None:intergenic | 35.0% | |
| CAAGCACTTATTTCAAGACT+TGG | + | Chr8:80869677-80869696 | MsG0880047006.01.T01:CDS | 35.0% | |
| TCATCAAGTTCATAAACTGC+TGG | - | Chr8:80869632-80869651 | None:intergenic | 35.0% | |
| ! | TCTGTTCCAACTGTTTTCAT+TGG | + | Chr8:80869710-80869729 | MsG0880047006.01.T01:CDS | 35.0% |
| GAACTTGATGAGATACCAAG+AGG | + | Chr8:80869641-80869660 | MsG0880047006.01.T01:CDS | 40.0% | |
| TCACCACCAATGAAAACAGT+TGG | - | Chr8:80869719-80869738 | None:intergenic | 40.0% | |
| TGTTCAATGTCTCTTCCTCT+TGG | - | Chr8:80869659-80869678 | None:intergenic | 40.0% | |
| ! | GTTCCAACTGTTTTCATTGG+TGG | + | Chr8:80869713-80869732 | MsG0880047006.01.T01:CDS | 40.0% |
| !! | TTCATTGGTGGTGAACTTGT+TGG | + | Chr8:80869725-80869744 | MsG0880047006.01.T01:CDS | 40.0% |
| CTCTTGCTGAATATCACCAC+TGG | - | Chr8:80869554-80869573 | None:intergenic | 45.0% | |
| !! | ATTGGTGGTGAACTTGTTGG+TGG | + | Chr8:80869728-80869747 | MsG0880047006.01.T01:CDS | 45.0% |
| GATGGTTTCAGAGAGACCAG+TGG | + | Chr8:80869535-80869554 | MsG0880047006.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 80869518 | 80869766 | 80869518 | ID=MsG0880047006.01;Name=MsG0880047006.01 |
| Chr8 | mRNA | 80869518 | 80869766 | 80869518 | ID=MsG0880047006.01.T01;Parent=MsG0880047006.01;Name=MsG0880047006.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|82 |
| Chr8 | exon | 80869518 | 80869766 | 80869518 | ID=MsG0880047006.01.T01:exon:34759;Parent=MsG0880047006.01.T01 |
| Chr8 | CDS | 80869518 | 80869766 | 80869518 | ID=MsG0880047006.01.T01:cds;Parent=MsG0880047006.01.T01 |
| Gene Sequence |
| Protein sequence |
