Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047004.01.T01 | XP_003609600.1 | 97.087 | 103 | 3 | 0 | 1 | 103 | 1 | 103 | 2.38E-67 | 207 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047004.01.T01 | Q8L8Z8 | 84.466 | 103 | 15 | 1 | 1 | 103 | 1 | 102 | 7.57E-60 | 181 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047004.01.T01 | G7JJ49 | 97.087 | 103 | 3 | 0 | 1 | 103 | 1 | 103 | 1.14e-67 | 207 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880047004.01 | MsG0880047005.01 | 0.939254 | 1.730713e-99 | 1.758724e-94 |
| MsG0880047004.01 | MsG0880047006.01 | 0.874185 | 8.227811e-68 | 3.668898e-64 |
| MsG0280007069.01 | MsG0880047004.01 | 0.867840 | 1.016794e-65 | 3.600608e-62 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047004.01.T01 | MTR_4g119050 | 97.087 | 103 | 3 | 0 | 1 | 103 | 1 | 103 | 2.88e-71 | 207 |
| MsG0880047004.01.T01 | MTR_4g119030 | 97.087 | 103 | 3 | 0 | 1 | 103 | 1 | 103 | 2.88e-71 | 207 |
| MsG0880047004.01.T01 | MTR_4g119040 | 97.087 | 103 | 3 | 0 | 1 | 103 | 1 | 103 | 2.88e-71 | 207 |
| MsG0880047004.01.T01 | MTR_2g019900 | 81.553 | 103 | 18 | 1 | 1 | 103 | 1 | 102 | 1.94e-58 | 175 |
| MsG0880047004.01.T01 | MTR_2g019950 | 65.049 | 103 | 35 | 1 | 1 | 103 | 1 | 102 | 8.57e-45 | 140 |
| MsG0880047004.01.T01 | MTR_1g088905 | 58.252 | 103 | 42 | 1 | 1 | 103 | 1 | 102 | 2.62e-41 | 132 |
| MsG0880047004.01.T01 | MTR_7g108250 | 56.311 | 103 | 44 | 1 | 1 | 103 | 1 | 102 | 1.01e-40 | 130 |
| MsG0880047004.01.T01 | MTR_7g026770 | 59.804 | 102 | 41 | 0 | 1 | 102 | 22 | 123 | 8.10e-40 | 134 |
| MsG0880047004.01.T01 | MTR_7g022710 | 54.369 | 103 | 47 | 0 | 1 | 103 | 30 | 132 | 2.55e-39 | 127 |
| MsG0880047004.01.T01 | MTR_7g022690 | 53.398 | 103 | 48 | 0 | 1 | 103 | 30 | 132 | 2.38e-38 | 125 |
| MsG0880047004.01.T01 | MTR_7g022550 | 52.427 | 103 | 49 | 0 | 1 | 103 | 30 | 132 | 1.65e-37 | 123 |
| MsG0880047004.01.T01 | MTR_7g108220 | 51.961 | 102 | 49 | 0 | 1 | 102 | 1 | 102 | 2.84e-34 | 114 |
| MsG0880047004.01.T01 | MTR_1g088910 | 53.922 | 102 | 46 | 1 | 1 | 102 | 1 | 101 | 5.80e-34 | 113 |
| MsG0880047004.01.T01 | MTR_7g108210 | 50.980 | 102 | 50 | 0 | 1 | 102 | 1 | 102 | 1.82e-33 | 112 |
| MsG0880047004.01.T01 | MTR_1g088920 | 52.083 | 96 | 45 | 1 | 1 | 96 | 1 | 95 | 5.59e-33 | 110 |
| MsG0880047004.01.T01 | MTR_1g088925 | 50.000 | 96 | 47 | 1 | 1 | 96 | 1 | 95 | 8.29e-33 | 110 |
| MsG0880047004.01.T01 | MTR_7g108200 | 50.000 | 96 | 47 | 1 | 1 | 96 | 1 | 95 | 2.15e-32 | 109 |
| MsG0880047004.01.T01 | MTR_1g088895 | 48.544 | 103 | 52 | 1 | 1 | 103 | 1 | 102 | 6.68e-30 | 103 |
| MsG0880047004.01.T01 | MTR_7g108260 | 44.118 | 102 | 56 | 1 | 1 | 102 | 1 | 101 | 7.04e-28 | 97.8 |
| MsG0880047004.01.T01 | MTR_2g090755 | 42.000 | 100 | 58 | 0 | 4 | 103 | 41 | 140 | 2.58e-25 | 92.4 |
| MsG0880047004.01.T01 | MTR_2g048970 | 46.667 | 105 | 53 | 1 | 2 | 103 | 29 | 133 | 1.21e-24 | 90.5 |
| MsG0880047004.01.T01 | MTR_2g014760 | 42.857 | 105 | 51 | 2 | 3 | 103 | 28 | 127 | 1.37e-24 | 90.5 |
| MsG0880047004.01.T01 | MTR_4g079110 | 43.689 | 103 | 48 | 3 | 6 | 103 | 58 | 155 | 2.66e-24 | 90.5 |
| MsG0880047004.01.T01 | MTR_1g015890 | 41.509 | 106 | 53 | 2 | 3 | 103 | 48 | 149 | 6.23e-21 | 81.6 |
| MsG0880047004.01.T01 | MTR_3g104510 | 37.624 | 101 | 56 | 2 | 3 | 102 | 23 | 117 | 7.93e-19 | 75.5 |
| MsG0880047004.01.T01 | MTR_7g035245 | 39.604 | 101 | 60 | 1 | 1 | 101 | 3 | 102 | 2.26e-18 | 73.9 |
| MsG0880047004.01.T01 | MTR_3g077570 | 35.644 | 101 | 64 | 1 | 1 | 101 | 18 | 117 | 2.32e-16 | 69.3 |
| MsG0880047004.01.T01 | MTR_1g069255 | 39.000 | 100 | 59 | 2 | 2 | 100 | 84 | 182 | 2.91e-16 | 70.5 |
| MsG0880047004.01.T01 | MTR_5g077550 | 40.777 | 103 | 60 | 1 | 2 | 103 | 21 | 123 | 2.36e-15 | 66.6 |
| MsG0880047004.01.T01 | MTR_3g077560 | 33.663 | 101 | 66 | 1 | 1 | 101 | 21 | 120 | 9.34e-15 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047004.01.T01 | AT5G18600 | 84.466 | 103 | 15 | 1 | 1 | 103 | 1 | 102 | 7.72e-61 | 181 |
| MsG0880047004.01.T01 | AT4G15690 | 78.641 | 103 | 21 | 1 | 1 | 103 | 1 | 102 | 5.29e-58 | 174 |
| MsG0880047004.01.T01 | AT4G15680 | 76.699 | 103 | 23 | 1 | 1 | 103 | 1 | 102 | 6.10e-58 | 174 |
| MsG0880047004.01.T01 | AT4G15700 | 76.699 | 103 | 23 | 1 | 1 | 103 | 1 | 102 | 3.42e-57 | 172 |
| MsG0880047004.01.T01 | AT4G15670 | 76.699 | 103 | 23 | 1 | 1 | 103 | 1 | 102 | 4.50e-57 | 171 |
| MsG0880047004.01.T01 | AT4G15660 | 75.728 | 103 | 24 | 1 | 1 | 103 | 1 | 102 | 5.03e-56 | 169 |
| MsG0880047004.01.T01 | AT1G03020 | 56.311 | 103 | 44 | 1 | 1 | 103 | 1 | 102 | 1.14e-41 | 132 |
| MsG0880047004.01.T01 | AT3G21460 | 61.165 | 103 | 39 | 1 | 1 | 103 | 1 | 102 | 2.13e-41 | 132 |
| MsG0880047004.01.T01 | AT3G62930 | 57.282 | 103 | 43 | 1 | 1 | 103 | 1 | 102 | 2.13e-41 | 132 |
| MsG0880047004.01.T01 | AT2G47870 | 52.427 | 103 | 49 | 0 | 1 | 103 | 1 | 103 | 3.77e-37 | 121 |
| MsG0880047004.01.T01 | AT1G06830 | 57.292 | 96 | 40 | 1 | 1 | 96 | 1 | 95 | 9.18e-37 | 120 |
| MsG0880047004.01.T01 | AT2G30540 | 57.292 | 96 | 40 | 1 | 1 | 96 | 1 | 95 | 1.44e-36 | 120 |
| MsG0880047004.01.T01 | AT3G62950 | 48.544 | 103 | 53 | 0 | 1 | 103 | 1 | 103 | 7.23e-35 | 115 |
| MsG0880047004.01.T01 | AT2G47880 | 54.167 | 96 | 43 | 1 | 1 | 96 | 1 | 95 | 8.25e-34 | 112 |
| MsG0880047004.01.T01 | AT5G14070 | 50.000 | 110 | 48 | 1 | 1 | 103 | 31 | 140 | 1.32e-33 | 113 |
| MsG0880047004.01.T01 | AT3G62960 | 52.083 | 96 | 45 | 1 | 1 | 96 | 1 | 95 | 3.09e-32 | 108 |
| MsG0880047004.01.T01 | AT3G02000 | 52.830 | 106 | 45 | 1 | 3 | 103 | 31 | 136 | 1.27e-29 | 103 |
| MsG0880047004.01.T01 | AT1G28480 | 38.095 | 105 | 62 | 1 | 2 | 103 | 33 | 137 | 2.74e-23 | 87.4 |
| MsG0880047004.01.T01 | AT4G33040 | 42.991 | 107 | 52 | 3 | 3 | 103 | 41 | 144 | 5.62e-22 | 84.3 |
| MsG0880047004.01.T01 | AT5G11930 | 41.584 | 101 | 48 | 3 | 3 | 102 | 57 | 147 | 1.16e-19 | 78.6 |
| MsG0880047004.01.T01 | AT1G03850 | 36.082 | 97 | 57 | 1 | 7 | 103 | 59 | 150 | 3.71e-18 | 74.7 |
| MsG0880047004.01.T01 | AT5G63030 | 38.384 | 99 | 60 | 1 | 3 | 101 | 21 | 118 | 1.18e-17 | 72.8 |
| MsG0880047004.01.T01 | AT2G20270 | 35.354 | 99 | 64 | 0 | 2 | 100 | 76 | 174 | 3.17e-15 | 67.8 |
| MsG0880047004.01.T01 | AT5G40370 | 34.653 | 101 | 65 | 1 | 1 | 101 | 3 | 102 | 5.19e-15 | 65.5 |
| MsG0880047004.01.T01 | AT4G28730 | 38.000 | 100 | 60 | 2 | 2 | 100 | 71 | 169 | 4.40e-14 | 64.7 |
| MsG0880047004.01.T01 | AT1G03850 | 32.927 | 82 | 50 | 1 | 7 | 88 | 59 | 135 | 8.54e-11 | 55.8 |
Find 13 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTCAATGTCTCTCCCTCT+TGG | 0.408003 | 8:-80843939 | None:intergenic |
| CAAGCACTTATTTCAAGACT+TGG | 0.435000 | 8:+80843960 | MsG0880047004.01.T01:CDS |
| TTCATTGGTGGTGAGCTTGT+TGG | 0.448261 | 8:+80844008 | MsG0880047004.01.T01:CDS |
| TCATCAAGTTCATAAACTGC+TGG | 0.480601 | 8:-80843912 | None:intergenic |
| ATTGGTGGTGAGCTTGTTGG+TGG | 0.517957 | 8:+80844011 | MsG0880047004.01.T01:CDS |
| ATGAAAACAGTTGGAACAGA+TGG | 0.519711 | 8:-80843990 | None:intergenic |
| CTCTTGCTGAATATCACCAC+TGG | 0.581366 | 8:-80843834 | None:intergenic |
| AATGGAGAGAGTAACAAAGA+TGG | 0.583009 | 8:+80843800 | None:intergenic |
| TAAGCATTGGAATTAAGGAA+CGG | 0.606032 | 8:-80844064 | None:intergenic |
| TCACCACCAATGAAAACAGT+TGG | 0.607302 | 8:-80843999 | None:intergenic |
| AACTTGATGAGATACCAAGA+GGG | 0.630472 | 8:+80843925 | MsG0880047004.01.T01:CDS |
| GAACTTGATGAGATACCAAG+AGG | 0.671905 | 8:+80843924 | MsG0880047004.01.T01:CDS |
| GATGGTTTCAGAGAGACCAG+TGG | 0.745891 | 8:+80843818 | MsG0880047004.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTAAGCATTGGAATTA+AGG | - | Chr8:80844072-80844091 | None:intergenic | 20.0% |
| ATTCCAATGCTTAAAAAAGC+TGG | + | Chr8:80844074-80844093 | MsG0880047004.01.T01:CDS | 30.0% | |
| ! | AAGACACTATTTTGTGACTT+TGG | + | Chr8:80843882-80843901 | MsG0880047004.01.T01:CDS | 30.0% |
| !! | TAAGCATTGGAATTAAGGAA+CGG | - | Chr8:80844067-80844086 | None:intergenic | 30.0% |
| AACTTGATGAGATACCAAGA+GGG | + | Chr8:80843925-80843944 | MsG0880047004.01.T01:CDS | 35.0% | |
| ATGAAAACAGTTGGAACAGA+TGG | - | Chr8:80843993-80844012 | None:intergenic | 35.0% | |
| CAAGCACTTATTTCAAGACT+TGG | + | Chr8:80843960-80843979 | MsG0880047004.01.T01:CDS | 35.0% | |
| TAAAAAAGCTGGTGCTCTTT+GGG | + | Chr8:80844085-80844104 | MsG0880047004.01.T01:CDS | 35.0% | |
| TCATCAAGTTCATAAACTGC+TGG | - | Chr8:80843915-80843934 | None:intergenic | 35.0% | |
| TTAAAAAAGCTGGTGCTCTT+TGG | + | Chr8:80844084-80844103 | MsG0880047004.01.T01:CDS | 35.0% | |
| ! | TCTGTTCCAACTGTTTTCAT+TGG | + | Chr8:80843993-80844012 | MsG0880047004.01.T01:CDS | 35.0% |
| GAACTTGATGAGATACCAAG+AGG | + | Chr8:80843924-80843943 | MsG0880047004.01.T01:CDS | 40.0% | |
| TCACCACCAATGAAAACAGT+TGG | - | Chr8:80844002-80844021 | None:intergenic | 40.0% | |
| ! | GTTCCAACTGTTTTCATTGG+TGG | + | Chr8:80843996-80844015 | MsG0880047004.01.T01:CDS | 40.0% |
| !!! | GCACCAGCTTTTTTAAGCAT+TGG | - | Chr8:80844080-80844099 | None:intergenic | 40.0% |
| CTCTTGCTGAATATCACCAC+TGG | - | Chr8:80843837-80843856 | None:intergenic | 45.0% | |
| TGTTCAATGTCTCTCCCTCT+TGG | - | Chr8:80843942-80843961 | None:intergenic | 45.0% | |
| !! | TTCATTGGTGGTGAGCTTGT+TGG | + | Chr8:80844008-80844027 | MsG0880047004.01.T01:CDS | 45.0% |
| GATGGTTTCAGAGAGACCAG+TGG | + | Chr8:80843818-80843837 | MsG0880047004.01.T01:CDS | 50.0% | |
| !! | ATTGGTGGTGAGCTTGTTGG+TGG | + | Chr8:80844011-80844030 | MsG0880047004.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 80843801 | 80844112 | 80843801 | ID=MsG0880047004.01;Name=MsG0880047004.01 |
| Chr8 | mRNA | 80843801 | 80844112 | 80843801 | ID=MsG0880047004.01.T01;Parent=MsG0880047004.01;Name=MsG0880047004.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|103 |
| Chr8 | exon | 80843801 | 80844112 | 80843801 | ID=MsG0880047004.01.T01:exon:34710;Parent=MsG0880047004.01.T01 |
| Chr8 | CDS | 80843801 | 80844112 | 80843801 | ID=MsG0880047004.01.T01:cds;Parent=MsG0880047004.01.T01 |
| Gene Sequence |
| Protein sequence |
