Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047005.01.T01 | XP_003609600.1 | 96.117 | 103 | 4 | 0 | 1 | 103 | 1 | 103 | 2.91E-66 | 204 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047005.01.T01 | Q8L8Z8 | 85.437 | 103 | 14 | 1 | 1 | 103 | 1 | 102 | 9.62E-61 | 183 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047005.01.T01 | G7JJ49 | 96.117 | 103 | 4 | 0 | 1 | 103 | 1 | 103 | 1.39e-66 | 204 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880047004.01 | MsG0880047005.01 | 0.939254 | 1.730713e-99 | 1.758724e-94 |
| MsG0880047005.01 | MsG0880047006.01 | 0.884966 | 1.220620e-71 | 8.224582e-68 |
| MsG0280007069.01 | MsG0880047005.01 | 0.840107 | 1.061375e-57 | 1.496520e-54 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047005.01.T01 | MTR_4g119050 | 96.117 | 103 | 4 | 0 | 1 | 103 | 1 | 103 | 3.52e-70 | 204 |
| MsG0880047005.01.T01 | MTR_4g119030 | 96.117 | 103 | 4 | 0 | 1 | 103 | 1 | 103 | 3.52e-70 | 204 |
| MsG0880047005.01.T01 | MTR_4g119040 | 96.117 | 103 | 4 | 0 | 1 | 103 | 1 | 103 | 3.52e-70 | 204 |
| MsG0880047005.01.T01 | MTR_2g019900 | 82.524 | 103 | 17 | 1 | 1 | 103 | 1 | 102 | 3.40e-59 | 177 |
| MsG0880047005.01.T01 | MTR_2g019950 | 65.049 | 103 | 35 | 1 | 1 | 103 | 1 | 102 | 9.15e-45 | 140 |
| MsG0880047005.01.T01 | MTR_1g088905 | 58.252 | 103 | 42 | 1 | 1 | 103 | 1 | 102 | 7.42e-42 | 133 |
| MsG0880047005.01.T01 | MTR_7g108250 | 56.311 | 103 | 44 | 1 | 1 | 103 | 1 | 102 | 4.06e-41 | 131 |
| MsG0880047005.01.T01 | MTR_7g026770 | 58.824 | 102 | 42 | 0 | 1 | 102 | 22 | 123 | 1.39e-38 | 130 |
| MsG0880047005.01.T01 | MTR_7g022710 | 53.398 | 103 | 48 | 0 | 1 | 103 | 30 | 132 | 8.36e-38 | 124 |
| MsG0880047005.01.T01 | MTR_7g022690 | 52.427 | 103 | 49 | 0 | 1 | 103 | 30 | 132 | 5.98e-37 | 121 |
| MsG0880047005.01.T01 | MTR_7g022550 | 51.456 | 103 | 50 | 0 | 1 | 103 | 30 | 132 | 4.57e-36 | 119 |
| MsG0880047005.01.T01 | MTR_7g108220 | 51.961 | 102 | 49 | 0 | 1 | 102 | 1 | 102 | 1.36e-34 | 115 |
| MsG0880047005.01.T01 | MTR_7g108210 | 50.980 | 102 | 50 | 0 | 1 | 102 | 1 | 102 | 8.47e-34 | 112 |
| MsG0880047005.01.T01 | MTR_1g088910 | 52.941 | 102 | 47 | 1 | 1 | 102 | 1 | 101 | 1.46e-32 | 109 |
| MsG0880047005.01.T01 | MTR_1g088920 | 51.042 | 96 | 46 | 1 | 1 | 96 | 1 | 95 | 8.17e-32 | 107 |
| MsG0880047005.01.T01 | MTR_1g088925 | 48.958 | 96 | 48 | 1 | 1 | 96 | 1 | 95 | 1.10e-31 | 107 |
| MsG0880047005.01.T01 | MTR_7g108200 | 48.958 | 96 | 48 | 1 | 1 | 96 | 1 | 95 | 4.08e-31 | 106 |
| MsG0880047005.01.T01 | MTR_1g088895 | 48.544 | 103 | 52 | 1 | 1 | 103 | 1 | 102 | 4.32e-30 | 103 |
| MsG0880047005.01.T01 | MTR_7g108260 | 43.137 | 102 | 57 | 1 | 1 | 102 | 1 | 101 | 9.39e-27 | 95.1 |
| MsG0880047005.01.T01 | MTR_2g090755 | 42.000 | 100 | 58 | 0 | 4 | 103 | 41 | 140 | 2.03e-25 | 92.8 |
| MsG0880047005.01.T01 | MTR_2g048970 | 46.667 | 105 | 53 | 1 | 2 | 103 | 29 | 133 | 3.65e-25 | 92.0 |
| MsG0880047005.01.T01 | MTR_2g014760 | 42.857 | 105 | 51 | 2 | 3 | 103 | 28 | 127 | 1.27e-24 | 90.5 |
| MsG0880047005.01.T01 | MTR_4g079110 | 43.689 | 103 | 48 | 3 | 6 | 103 | 58 | 155 | 2.49e-24 | 90.5 |
| MsG0880047005.01.T01 | MTR_1g015890 | 41.509 | 106 | 53 | 2 | 3 | 103 | 48 | 149 | 5.30e-21 | 81.6 |
| MsG0880047005.01.T01 | MTR_7g035245 | 40.594 | 101 | 59 | 1 | 1 | 101 | 3 | 102 | 4.23e-19 | 75.9 |
| MsG0880047005.01.T01 | MTR_3g104510 | 37.624 | 101 | 56 | 2 | 3 | 102 | 23 | 117 | 6.11e-19 | 75.9 |
| MsG0880047005.01.T01 | MTR_3g077570 | 36.634 | 101 | 63 | 1 | 1 | 101 | 18 | 117 | 4.19e-17 | 71.2 |
| MsG0880047005.01.T01 | MTR_5g077550 | 40.777 | 103 | 60 | 1 | 2 | 103 | 21 | 123 | 1.06e-15 | 67.4 |
| MsG0880047005.01.T01 | MTR_1g069255 | 37.374 | 99 | 62 | 0 | 2 | 100 | 84 | 182 | 1.43e-15 | 68.6 |
| MsG0880047005.01.T01 | MTR_3g077560 | 34.653 | 101 | 65 | 1 | 1 | 101 | 21 | 120 | 1.93e-15 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047005.01.T01 | AT5G18600 | 85.437 | 103 | 14 | 1 | 1 | 103 | 1 | 102 | 9.80e-62 | 183 |
| MsG0880047005.01.T01 | AT4G15690 | 78.641 | 103 | 21 | 1 | 1 | 103 | 1 | 102 | 1.63e-58 | 175 |
| MsG0880047005.01.T01 | AT4G15680 | 76.699 | 103 | 23 | 1 | 1 | 103 | 1 | 102 | 2.15e-58 | 175 |
| MsG0880047005.01.T01 | AT4G15700 | 77.670 | 103 | 22 | 1 | 1 | 103 | 1 | 102 | 4.20e-58 | 174 |
| MsG0880047005.01.T01 | AT4G15670 | 76.699 | 103 | 23 | 1 | 1 | 103 | 1 | 102 | 1.53e-57 | 172 |
| MsG0880047005.01.T01 | AT4G15660 | 75.728 | 103 | 24 | 1 | 1 | 103 | 1 | 102 | 1.85e-56 | 170 |
| MsG0880047005.01.T01 | AT1G03020 | 57.282 | 103 | 43 | 1 | 1 | 103 | 1 | 102 | 2.25e-42 | 134 |
| MsG0880047005.01.T01 | AT3G62930 | 58.252 | 103 | 42 | 1 | 1 | 103 | 1 | 102 | 4.91e-42 | 133 |
| MsG0880047005.01.T01 | AT3G21460 | 61.165 | 103 | 39 | 1 | 1 | 103 | 1 | 102 | 1.64e-41 | 132 |
| MsG0880047005.01.T01 | AT2G47870 | 51.456 | 103 | 50 | 0 | 1 | 103 | 1 | 103 | 6.50e-36 | 118 |
| MsG0880047005.01.T01 | AT1G06830 | 56.250 | 96 | 41 | 1 | 1 | 96 | 1 | 95 | 1.69e-35 | 117 |
| MsG0880047005.01.T01 | AT2G30540 | 56.250 | 96 | 41 | 1 | 1 | 96 | 1 | 95 | 2.27e-35 | 117 |
| MsG0880047005.01.T01 | AT3G62950 | 47.573 | 103 | 54 | 0 | 1 | 103 | 1 | 103 | 1.71e-33 | 112 |
| MsG0880047005.01.T01 | AT2G47880 | 53.125 | 96 | 44 | 1 | 1 | 96 | 1 | 95 | 1.50e-32 | 109 |
| MsG0880047005.01.T01 | AT5G14070 | 49.091 | 110 | 49 | 1 | 1 | 103 | 31 | 140 | 2.68e-32 | 110 |
| MsG0880047005.01.T01 | AT3G62960 | 51.042 | 96 | 46 | 1 | 1 | 96 | 1 | 95 | 6.00e-31 | 105 |
| MsG0880047005.01.T01 | AT3G02000 | 51.887 | 106 | 46 | 1 | 3 | 103 | 31 | 136 | 3.89e-28 | 99.8 |
| MsG0880047005.01.T01 | AT1G28480 | 38.095 | 105 | 62 | 1 | 2 | 103 | 33 | 137 | 1.87e-23 | 87.8 |
| MsG0880047005.01.T01 | AT4G33040 | 43.810 | 105 | 54 | 2 | 3 | 103 | 41 | 144 | 3.45e-22 | 84.7 |
| MsG0880047005.01.T01 | AT5G11930 | 41.584 | 101 | 48 | 3 | 3 | 102 | 57 | 147 | 9.83e-20 | 78.6 |
| MsG0880047005.01.T01 | AT5G63030 | 39.394 | 99 | 59 | 1 | 3 | 101 | 21 | 118 | 2.52e-18 | 74.3 |
| MsG0880047005.01.T01 | AT1G03850 | 36.082 | 97 | 57 | 1 | 7 | 103 | 59 | 150 | 4.14e-18 | 74.3 |
| MsG0880047005.01.T01 | AT5G40370 | 35.644 | 101 | 64 | 1 | 1 | 101 | 3 | 102 | 1.14e-15 | 67.0 |
| MsG0880047005.01.T01 | AT2G20270 | 35.354 | 99 | 64 | 0 | 2 | 100 | 76 | 174 | 2.05e-15 | 68.2 |
| MsG0880047005.01.T01 | AT4G28730 | 36.364 | 99 | 63 | 0 | 2 | 100 | 71 | 169 | 1.82e-13 | 63.2 |
| MsG0880047005.01.T01 | AT1G03850 | 32.927 | 82 | 50 | 1 | 7 | 88 | 59 | 135 | 9.00e-11 | 55.8 |
| MsG0880047005.01.T01 | AT5G40370 | 36.782 | 87 | 54 | 1 | 15 | 101 | 42 | 127 | 9.67e-11 | 55.1 |
Find 11 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGCACTTATTTCAAGACT+TGG | 0.435000 | 8:+80855991 | MsG0880047005.01.T01:CDS |
| TTCATTGGTGGTGAGCTTGT+TGG | 0.448261 | 8:+80856039 | MsG0880047005.01.T01:CDS |
| TCATCAAGTTCATAAACTGC+TGG | 0.480601 | 8:-80855943 | None:intergenic |
| ATTGGTGGTGAGCTTGTTGG+TGG | 0.517957 | 8:+80856042 | MsG0880047005.01.T01:CDS |
| ATGAAAACAGTTGGAACAGA+TGG | 0.519711 | 8:-80856021 | None:intergenic |
| GAACTTGATGAGATATCAAG+AGG | 0.572963 | 8:+80855955 | MsG0880047005.01.T01:CDS |
| CTCTTGCTGAATATCACCAC+TGG | 0.581366 | 8:-80855865 | None:intergenic |
| AATGGAGAGAGTAACAAAGA+TGG | 0.583009 | 8:+80855831 | None:intergenic |
| TAAGCATTGGAATTAAGGAA+CGG | 0.606032 | 8:-80856095 | None:intergenic |
| TCACCACCAATGAAAACAGT+TGG | 0.607302 | 8:-80856030 | None:intergenic |
| GATGGTTTCAGAGAGACCAG+TGG | 0.745891 | 8:+80855849 | MsG0880047005.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTAAGCATTGGAATTA+AGG | - | Chr8:80856103-80856122 | None:intergenic | 20.0% |
| ATTCCAATGCTTAAAAAAGC+TGG | + | Chr8:80856105-80856124 | MsG0880047005.01.T01:CDS | 30.0% | |
| ! | AAGACACTATTTTGTGACTT+TGG | + | Chr8:80855913-80855932 | MsG0880047005.01.T01:CDS | 30.0% |
| !! | TAAGCATTGGAATTAAGGAA+CGG | - | Chr8:80856098-80856117 | None:intergenic | 30.0% |
| ATGAAAACAGTTGGAACAGA+TGG | - | Chr8:80856024-80856043 | None:intergenic | 35.0% | |
| CAAGCACTTATTTCAAGACT+TGG | + | Chr8:80855991-80856010 | MsG0880047005.01.T01:CDS | 35.0% | |
| GAACTTGATGAGATATCAAG+AGG | + | Chr8:80855955-80855974 | MsG0880047005.01.T01:CDS | 35.0% | |
| TAAAAAAGCTGGAGCTCTTT+GGG | + | Chr8:80856116-80856135 | MsG0880047005.01.T01:CDS | 35.0% | |
| TCATCAAGTTCATAAACTGC+TGG | - | Chr8:80855946-80855965 | None:intergenic | 35.0% | |
| TTAAAAAAGCTGGAGCTCTT+TGG | + | Chr8:80856115-80856134 | MsG0880047005.01.T01:CDS | 35.0% | |
| ! | TCTGTTCCAACTGTTTTCAT+TGG | + | Chr8:80856024-80856043 | MsG0880047005.01.T01:CDS | 35.0% |
| TCACCACCAATGAAAACAGT+TGG | - | Chr8:80856033-80856052 | None:intergenic | 40.0% | |
| ! | GTTCCAACTGTTTTCATTGG+TGG | + | Chr8:80856027-80856046 | MsG0880047005.01.T01:CDS | 40.0% |
| !!! | GCTCCAGCTTTTTTAAGCAT+TGG | - | Chr8:80856111-80856130 | None:intergenic | 40.0% |
| CTCTTGCTGAATATCACCAC+TGG | - | Chr8:80855868-80855887 | None:intergenic | 45.0% | |
| !! | TTCATTGGTGGTGAGCTTGT+TGG | + | Chr8:80856039-80856058 | MsG0880047005.01.T01:CDS | 45.0% |
| GATGGTTTCAGAGAGACCAG+TGG | + | Chr8:80855849-80855868 | MsG0880047005.01.T01:CDS | 50.0% | |
| !! | ATTGGTGGTGAGCTTGTTGG+TGG | + | Chr8:80856042-80856061 | MsG0880047005.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 80855832 | 80856143 | 80855832 | ID=MsG0880047005.01;Name=MsG0880047005.01 |
| Chr8 | mRNA | 80855832 | 80856143 | 80855832 | ID=MsG0880047005.01.T01;Parent=MsG0880047005.01;Name=MsG0880047005.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|103 |
| Chr8 | exon | 80855832 | 80856143 | 80855832 | ID=MsG0880047005.01.T01:exon:34711;Parent=MsG0880047005.01.T01 |
| Chr8 | CDS | 80855832 | 80856143 | 80855832 | ID=MsG0880047005.01.T01:cds;Parent=MsG0880047005.01.T01 |
| Gene Sequence |
| Protein sequence |
