AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047277.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047277.01.T02 MTR_4g124260 54.962 131 15 4 5 91 110 240 1.30e-31 110
MsG0880047277.01.T01 MTR_4g124260 94.393 107 6 0 1 107 1 107 2.44e-71 213
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047277.01.T02 AT3G17590 44.915 118 34 4 5 91 110 227 1.05e-19 79.7
MsG0880047277.01.T02 AT3G17590 41.985 131 32 5 5 91 110 240 6.81e-18 75.5
MsG0880047277.01.T02 AT3G17590 41.985 131 32 5 5 91 112 242 7.16e-18 75.5
MsG0880047277.01.T01 AT3G17590 76.190 105 25 0 1 105 1 105 9.97e-57 175
MsG0880047277.01.T01 AT3G17590 74.766 107 27 0 1 107 1 107 1.40e-56 175
MsG0880047277.01.T01 AT3G17590 72.477 109 28 1 1 107 1 109 8.00e-54 168

Find 92 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTAATTAATTAATTAATT+TGG 0.138415 8:-84068287 MsG0880047277.01.T01:five_prime_UTR
GCGCTTATATAAGGTGTTTA+TGG 0.245494 8:+84067554 None:intergenic
TAAGGTTGTCAAGGTACTTA+TGG 0.259956 8:+84067242 None:intergenic
GCCTTCACTTGGAACCCTTC+TGG 0.293675 8:-84067984 MsG0880047277.01.T01:intron
AGCTACTATAAGAGAACTTA+TGG 0.297207 8:-84067478 MsG0880047277.01.T01:three_prime_UTR
GAAGGTTCTTGCAACTCTTC+AGG 0.302745 8:+84066452 None:intergenic
TAATTAATTAATTTGGTTCC+TGG 0.320333 8:-84068280 MsG0880047277.01.T01:five_prime_UTR
GTCTACATTTGTTAAATTCC+AGG 0.324512 8:-84066498 MsG0880047277.01.T01:intron
TCAAATTAGTCAATCCAAAA+AGG 0.328158 8:-84067183 MsG0880047277.01.T01:three_prime_UTR
TTATGGCTGGCCAGTATATA+AGG 0.345681 8:-84067381 MsG0880047277.01.T01:three_prime_UTR
AAAGGGATGATAAGAATCTA+TGG 0.350609 8:+84067160 None:intergenic
TATCCATGAGCGCTTATATA+AGG 0.369121 8:+84067545 None:intergenic
TTTCTGTAGAAAGCAGAAAC+TGG 0.372807 8:+84068165 None:intergenic
TCCAAATGGGCCTTATATAC+TGG 0.380887 8:+84067371 None:intergenic
AAAAGGGCAGGCTTCTTTGA+TGG 0.387400 8:+84067114 None:intergenic
GCCAGTATATAAGGCCCATT+TGG 0.387564 8:-84067372 MsG0880047277.01.T01:three_prime_UTR
TGAACAGTTTGTAAGTGTTA+TGG 0.392177 8:-84067398 MsG0880047277.01.T01:three_prime_UTR
ATATAAGGTGTTTATGGAAC+AGG 0.398502 8:+84067560 None:intergenic
ACAGAAACCCTGTCAAGTTC+AGG 0.399238 8:-84068149 MsG0880047277.01.T01:CDS
CATTGGTCATTGAAAAGAAA+AGG 0.406769 8:+84067056 None:intergenic
AAGGTTCGTTTACATCATAA+TGG 0.407538 8:-84066819 MsG0880047277.01.T01:three_prime_UTR
CATTGATCATTGAAAAGAAA+AGG 0.411585 8:+84067097 None:intergenic
AACACTACAATCTCAGAATC+AGG 0.412173 8:+84067859 None:intergenic
AGAAGAGAAATGTACTTGAA+TGG 0.412442 8:+84067774 None:intergenic
TATGTTCCTTGGATTGAACT+AGG 0.427896 8:-84067588 MsG0880047277.01.T01:exon
AGCACATTTCATTTCTCTAC+GGG 0.436689 8:-84066786 MsG0880047277.01.T01:three_prime_UTR
GAGAGAGAGGCAGACCTTGA+TGG 0.438619 8:+84067615 None:intergenic
GGGCTGACTTCTTTCCAGTC+AGG 0.438902 8:-84065995 MsG0880047277.01.T01:intron
AAAGGGCAGGCTTCTTTGAT+GGG 0.446434 8:+84067115 None:intergenic
GTCGCTTGTAATCATATCAT+TGG 0.449054 8:+84067039 None:intergenic
TGTTCAGTTGTTCGTTCACC+AGG 0.457968 8:+84068262 None:intergenic
ATACATATCTTGACCGTCAT+AGG 0.457986 8:+84067657 None:intergenic
ACTTACATCCTGAACTTGAC+AGG 0.473616 8:+84068141 None:intergenic
ATTTCAATGTCGAGTCGAAT+GGG 0.476706 8:+84068029 None:intergenic
ATTGATCATTGAAAAGAAAA+GGG 0.477653 8:+84067098 None:intergenic
ATCATTGAAAAGAAAAGGGC+AGG 0.478951 8:+84067102 None:intergenic
ATGTTCCTTGGATTGAACTA+GGG 0.478977 8:-84067587 MsG0880047277.01.T01:exon
TGAGCAATTTGAGTAACAAA+AGG 0.480272 8:+84067799 None:intergenic
CAGCACATTTCATTTCTCTA+CGG 0.486201 8:-84066787 MsG0880047277.01.T01:three_prime_UTR
ATTGGTCATTGAAAAGAAAA+GGG 0.486532 8:+84067057 None:intergenic
TTCTGTAGAAAGCAGAAACT+GGG 0.489716 8:+84068166 None:intergenic
GATTTCAATGTCGAGTCGAA+TGG 0.493002 8:+84068028 None:intergenic
ATGTATCAAAATTACGTACT+TGG 0.497307 8:-84066903 MsG0880047277.01.T01:three_prime_UTR
TATCAAAGTTGTTCAAGTCC+TGG 0.497805 8:+84066480 None:intergenic
GACGGTCAAGATATGTATGC+TGG 0.501652 8:-84067652 MsG0880047277.01.T01:CDS
TCTGATGGCAAAGGCAATAG+TGG 0.504411 8:+84066241 None:intergenic
ACAATTTATGAACATGCCAT+AGG 0.510040 8:-84067443 MsG0880047277.01.T01:three_prime_UTR
ACACCTTATATAAGCGCTCA+TGG 0.515892 8:-84067548 MsG0880047277.01.T01:three_prime_UTR
TGTAAATCATACGCTTGTAA+AGG 0.524144 8:-84066621 MsG0880047277.01.T01:three_prime_UTR
GGCATTGAGGATCCTGAGGT+TGG 0.528970 8:-84066417 MsG0880047277.01.T01:three_prime_UTR
GAAAGCAGATTAAGCAAGAA+GGG 0.529838 8:-84066026 MsG0880047277.01.T01:three_prime_UTR
CTGCAGAGACATGGGCATTG+AGG 0.530287 8:-84066430 MsG0880047277.01.T01:three_prime_UTR
AGAGAGAGGCAGACCTTGAT+GGG 0.535777 8:+84067616 None:intergenic
AGAAAGCAGATTAAGCAAGA+AGG 0.537341 8:-84066027 MsG0880047277.01.T01:three_prime_UTR
AAGAACCTTCTGCAGAGACA+TGG 0.540639 8:-84066439 MsG0880047277.01.T01:three_prime_UTR
CCTCGTAAAGCTGTCTCTGA+TGG 0.545433 8:+84066226 None:intergenic
GCAATTTGAGTAACAAAAGG+TGG 0.545843 8:+84067802 None:intergenic
ACCAGAAGGGTTCCAAGTGA+AGG 0.556476 8:+84067983 None:intergenic
GATACAAGGATGCCTTCACT+TGG 0.562726 8:-84067995 MsG0880047277.01.T01:CDS
AGAGAAGATTGTTCCCATCA+AGG 0.563391 8:-84067629 MsG0880047277.01.T01:CDS
ATTCAATGATCATGCAATGA+GGG 0.563563 8:+84068217 None:intergenic
GCTGAATTTCGATCCTATGA+CGG 0.564430 8:-84067670 MsG0880047277.01.T01:CDS
AATGCCCATGTCTCTGCAGA+AGG 0.565956 8:+84066434 None:intergenic
CTTACATCCTGAACTTGACA+GGG 0.569157 8:+84068142 None:intergenic
AAGGGCAGGCTTCTTTGATG+GGG 0.572306 8:+84067116 None:intergenic
GATATACTTACTCCAACCTC+AGG 0.574479 8:+84066405 None:intergenic
TGTCGTTTAAGCATCTCTCA+AGG 0.585034 8:-84066727 MsG0880047277.01.T01:three_prime_UTR
TCAGATTGTAGTTCAGAGTG+TGG 0.590053 8:-84066060 MsG0880047277.01.T01:intron
ATGATCATTGAATATTGAGA+AGG 0.592438 8:-84068207 MsG0880047277.01.T01:five_prime_UTR
AGAACCTTCTGCAGAGACAT+GGG 0.593998 8:-84066438 MsG0880047277.01.T01:three_prime_UTR
CATAATTCATAAGGTTGTCA+AGG 0.599040 8:+84067233 None:intergenic
TATTCAATGATCATGCAATG+AGG 0.603253 8:+84068216 None:intergenic
TCATTGAATATTGAGAAGGT+AGG 0.610483 8:-84068203 MsG0880047277.01.T01:five_prime_UTR
CAAAACACACAGGCCAACCT+CGG 0.612239 8:-84068063 MsG0880047277.01.T01:intron
TAAGCAAGAAGGGTCGTCGA+GGG 0.614091 8:-84066016 MsG0880047277.01.T01:three_prime_UTR
GTCGTTTAAGCATCTCTCAA+GGG 0.615629 8:-84066726 MsG0880047277.01.T01:three_prime_UTR
ATTTGAGTAACAAAAGGTGG+AGG 0.622715 8:+84067805 None:intergenic
AAAGCTGTCTCTGATGGCAA+AGG 0.623544 8:+84066232 None:intergenic
CAGTTTGTAAGTGTTATGGC+TGG 0.624722 8:-84067394 MsG0880047277.01.T01:three_prime_UTR
AATCGACGGTCATAGATACA+AGG 0.627696 8:-84068009 MsG0880047277.01.T01:CDS
AACACTTACAAACTGTTCAG+AGG 0.630863 8:+84067402 None:intergenic
TAAGGTGTTTATGGAACAGG+AGG 0.635802 8:+84067563 None:intergenic
CATGGGCATTGAGGATCCTG+AGG 0.640210 8:-84066421 MsG0880047277.01.T01:three_prime_UTR
CCATCAGAGACAGCTTTACG+AGG 0.644745 8:-84066226 MsG0880047277.01.T01:intron
GAGGACCCTAGTTCAATCCA+AGG 0.648329 8:+84067582 None:intergenic
AACAGAACATAAGATAGCAT+CGG 0.649914 8:-84068243 MsG0880047277.01.T01:five_prime_UTR
TTAAGCAAGAAGGGTCGTCG+AGG 0.656519 8:-84066017 MsG0880047277.01.T01:three_prime_UTR
AAGCAAGAAGGGTCGTCGAG+GGG 0.658726 8:-84066015 MsG0880047277.01.T01:three_prime_UTR
AGGGCAGGCTTCTTTGATGG+GGG 0.672653 8:+84067117 None:intergenic
TCTGTAGAAAGCAGAAACTG+GGG 0.674757 8:+84068167 None:intergenic
CGACTCGACATTGAAATCGA+CGG 0.674810 8:-84068023 MsG0880047277.01.T01:CDS
GCACATTTCATTTCTCTACG+GGG 0.695678 8:-84066785 MsG0880047277.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATATATATA+TGG - Chr8:84066930-84066949 MsG0880047277.01.T01:three_prime_UTR 0.0%
!! ATTAAAAAACTATATAAGAA+TGG + Chr8:84065164-84065183 None:intergenic 10.0%
!! ATTAATTTATTATTAGCAAT+AGG + Chr8:84066712-84066731 None:intergenic 10.0%
!! TATTATTAATTAATTTCACT+TGG - Chr8:84065136-84065155 MsG0880047277.01.T01:intron 10.0%
!!! TATGTTTGTATTTTATTTTA+TGG - Chr8:84067941-84067960 MsG0880047277.01.T01:intron 10.0%
!! AAATTATAATATAAGCTAAG+TGG + Chr8:84067906-84067925 None:intergenic 15.0%
!! AATAATAAAAAAATACCAGA+AGG + Chr8:84065123-84065142 None:intergenic 15.0%
!! AATACAAACATAATCTTAAA+AGG + Chr8:84067933-84067952 None:intergenic 15.0%
!! ATAATAAAAAAATACCAGAA+GGG + Chr8:84065122-84065141 None:intergenic 15.0%
!! CTTATAAAAAAAAAAACCTA+TGG + Chr8:84065665-84065684 None:intergenic 15.0%
!! TAAAACCTATAGTAATAATA+GGG + Chr8:84067456-84067475 None:intergenic 15.0%
!! TCACACATTTATAAAAAAAA+TGG + Chr8:84066111-84066130 None:intergenic 15.0%
!! TTAAAACCTATAGTAATAAT+AGG + Chr8:84067457-84067476 None:intergenic 15.0%
!! TTTATAAACAAATTTGATGA+TGG - Chr8:84067622-84067641 MsG0880047277.01.T01:CDS 15.0%
!!! GTAATTTTGATACATTTATT+TGG + Chr8:84066181-84066200 None:intergenic 15.0%
!!! TTCAACTTATTTGTAATTTT+TGG - Chr8:84067804-84067823 MsG0880047277.01.T01:CDS 15.0%
!! AAATTCAAGTCCATAATAAT+GGG + Chr8:84066765-84066784 None:intergenic 20.0%
!! AATTTATTATTAGCAATAGG+TGG + Chr8:84066709-84066728 None:intergenic 20.0%
!! ATGAACTAACAGATATTAAT+AGG + Chr8:84067601-84067620 None:intergenic 20.0%
!! ATTGATCATTGAAAAGAAAA+GGG + Chr8:84065994-84066013 None:intergenic 20.0%
!! GTATGTTTCAAATTTCAAAA+AGG + Chr8:84066800-84066819 None:intergenic 20.0%
!!! AGAGTGTAAATTAGTTTTAA+AGG + Chr8:84065950-84065969 None:intergenic 20.0%
!!! AGTGTATTTTTTACAATTAG+AGG - Chr8:84066332-84066351 MsG0880047277.01.T01:intron 20.0%
!!! AGTTAAAGTTCTTTTTCATA+TGG - Chr8:84067414-84067433 MsG0880047277.01.T01:three_prime_UTR 20.0%
!!! ATAATTTTCAATGTCATTGA+TGG + Chr8:84066215-84066234 None:intergenic 20.0%
!!! GAAAATTATACACATGTTTT+TGG - Chr8:84066226-84066245 MsG0880047277.01.T01:intron 20.0%
!!! GAGTGTAAATTAGTTTTAAA+GGG + Chr8:84065949-84065968 None:intergenic 20.0%
!!! TAATTAATTAATTTGGTTCC+TGG - Chr8:84064809-84064828 MsG0880047277.01.T01:three_prime_UTR 20.0%
! AAAAACAAGTCAATCCAAAT+GGG + Chr8:84065734-84065753 None:intergenic 25.0%
! AGAAAGAAAGAAAGAAAGAA+AGG + Chr8:84064975-84064994 None:intergenic 25.0%
! AGTTTCCCTATTATTACTAT+AGG - Chr8:84067448-84067467 MsG0880047277.01.T01:three_prime_UTR 25.0%
! ATGAAAATGAATTAAGACGA+TGG + Chr8:84067007-84067026 None:intergenic 25.0%
! ATGATCATTGAATATTGAGA+AGG - Chr8:84064882-84064901 MsG0880047277.01.T01:three_prime_UTR 25.0%
! ATGTATCAAAATTACGTACT+TGG - Chr8:84066186-84066205 MsG0880047277.01.T01:intron 25.0%
! ATTGGTCATTGAAAAGAAAA+GGG + Chr8:84066035-84066054 None:intergenic 25.0%
! CAAATTCAAGTCCATAATAA+TGG + Chr8:84066766-84066785 None:intergenic 25.0%
! CATTGATCATTGAAAAGAAA+AGG + Chr8:84065995-84066014 None:intergenic 25.0%
! TCAAATTAGTCAATCCAAAA+AGG - Chr8:84065906-84065925 MsG0880047277.01.T01:intron 25.0%
! TGTAATAGTACCCATTATTA+TGG - Chr8:84066752-84066771 MsG0880047277.01.T01:three_prime_UTR 25.0%
! TTAGCTAATTTGAACATACA+AGG - Chr8:84067760-84067779 MsG0880047277.01.T01:intron 25.0%
! TTTATCATATCAACTGAACT+AGG + Chr8:84067138-84067157 None:intergenic 25.0%
!! GTATTAAATCTAGTGTTGTT+CGG - Chr8:84067279-84067298 MsG0880047277.01.T01:three_prime_UTR 25.0%
!! TGTAAAGGACCAATTTTTAT+GGG - Chr8:84066483-84066502 MsG0880047277.01.T01:intron 25.0%
!! TTGTAAAGGACCAATTTTTA+TGG - Chr8:84066482-84066501 MsG0880047277.01.T01:intron 25.0%
!!! AGGCTATAACTACTATTTTT+GGG + Chr8:84067509-84067528 None:intergenic 25.0%
!!! AGTATACTAGTTGGTTTTAT+AGG - Chr8:84068214-84068233 MsG0880047277.01.T01:five_prime_UTR 25.0%
!!! ATACACATGTTTTTGGAAAT+TGG - Chr8:84066233-84066252 MsG0880047277.01.T01:three_prime_UTR 25.0%
!!! ATTGGAAGACTTGTTTTAAA+AGG - Chr8:84066251-84066270 MsG0880047277.01.T01:intron 25.0%
!!! ATTTTTTACAATTAGAGGAG+AGG - Chr8:84066337-84066356 MsG0880047277.01.T01:intron 25.0%
!!! TTTTTGAGCTTATGAAAAGT+AGG - Chr8:84065749-84065768 MsG0880047277.01.T01:intron 25.0%
AAAGGGATGATAAGAATCTA+TGG + Chr8:84065932-84065951 None:intergenic 30.0%
AACAGAACATAAGATAGCAT+CGG - Chr8:84064846-84064865 MsG0880047277.01.T01:three_prime_UTR 30.0%
AAGGTTCGTTTACATCATAA+TGG - Chr8:84066270-84066289 MsG0880047277.01.T01:intron 30.0%
ACAATTTATGAACATGCCAT+AGG - Chr8:84065646-84065665 MsG0880047277.01.T01:intron 30.0%
ACATGTACTAGTATACTAGT+TGG - Chr8:84068205-84068224 MsG0880047277.01.T01:five_prime_UTR 30.0%
AGAAGAGAAATGTACTTGAA+TGG + Chr8:84065318-84065337 None:intergenic 30.0%
AGCTACTATAAGAGAACTTA+TGG - Chr8:84065611-84065630 MsG0880047277.01.T01:intron 30.0%
ATTCAATGATCATGCAATGA+GGG + Chr8:84064875-84064894 None:intergenic 30.0%
CAAAAACAAGTCAATCCAAA+TGG + Chr8:84065735-84065754 None:intergenic 30.0%
CATAATTCATAAGGTTGTCA+AGG + Chr8:84065859-84065878 None:intergenic 30.0%
CATTGGTCATTGAAAAGAAA+AGG + Chr8:84066036-84066055 None:intergenic 30.0%
CCAATCATATAATACAATGC+CGG + Chr8:84066522-84066541 None:intergenic 30.0%
GATGATCAATTCATGTTTGT+TGG - Chr8:84065370-84065389 MsG0880047277.01.T01:intron 30.0%
GCAATCTTACCCATAAAAAT+TGG + Chr8:84066495-84066514 None:intergenic 30.0%
TAACGATAAACAAAACACAC+AGG - Chr8:84065016-84065035 MsG0880047277.01.T01:three_prime_UTR 30.0%
TATTCAATGATCATGCAATG+AGG + Chr8:84064876-84064895 None:intergenic 30.0%
TCATTGAATATTGAGAAGGT+AGG - Chr8:84064886-84064905 MsG0880047277.01.T01:three_prime_UTR 30.0%
TGAACAGTTTGTAAGTGTTA+TGG - Chr8:84065691-84065710 MsG0880047277.01.T01:intron 30.0%
TGAGCAATTTGAGTAACAAA+AGG + Chr8:84065293-84065312 None:intergenic 30.0%
TGGGTACTATTACAAATTCA+AGG + Chr8:84066746-84066765 None:intergenic 30.0%
TGTAAATCATACGCTTGTAA+AGG - Chr8:84066468-84066487 MsG0880047277.01.T01:three_prime_UTR 30.0%
TTAGCTAAAATTGCACAAAC+AGG + Chr8:84067748-84067767 None:intergenic 30.0%
TTCTACTGAACTATCTGATT+AGG + Chr8:84068151-84068170 None:intergenic 30.0%
TTGATATAATCCGATCATCT+TGG - Chr8:84067366-84067385 MsG0880047277.01.T01:three_prime_UTR 30.0%
! AACAACGAGATTTTGTCAAT+AGG + Chr8:84066428-84066447 None:intergenic 30.0%
! ATAAGAATCTATGGCCTTTT+TGG + Chr8:84065923-84065942 None:intergenic 30.0%
! ATATAAGGTGTTTATGGAAC+AGG + Chr8:84065532-84065551 None:intergenic 30.0%
! GAGGCTATAACTACTATTTT+TGG + Chr8:84067510-84067529 None:intergenic 30.0%
! GCTTTTACGCATAATTCATA+AGG + Chr8:84065868-84065887 None:intergenic 30.0%
! GTCTACATTTGTTAAATTCC+AGG - Chr8:84066591-84066610 MsG0880047277.01.T01:intron 30.0%
! TTCTTATGCTTTGACTGTTT+TGG - Chr8:84067991-84068010 MsG0880047277.01.T01:CDS 30.0%
!!! TAACTACTATTTTTGGGCAT+AGG + Chr8:84067503-84067522 None:intergenic 30.0%
AACACTACAATCTCAGAATC+AGG + Chr8:84065233-84065252 None:intergenic 35.0%
AACACTTACAAACTGTTCAG+AGG + Chr8:84065690-84065709 None:intergenic 35.0%
AAGTGTATGAAAAACCTGAC+TGG + Chr8:84067111-84067130 None:intergenic 35.0%
AGAAAGCAGATTAAGCAAGA+AGG - Chr8:84067062-84067081 MsG0880047277.01.T01:three_prime_UTR 35.0%
AGCACATTTCATTTCTCTAC+GGG - Chr8:84066303-84066322 MsG0880047277.01.T01:intron 35.0%
AGGAACATAAAAAGAGAGAG+AGG + Chr8:84065490-84065509 None:intergenic 35.0%
ATACATATCTTGACCGTCAT+AGG + Chr8:84065435-84065454 None:intergenic 35.0%
ATCATTGAAAAGAAAAGGGC+AGG + Chr8:84065990-84066009 None:intergenic 35.0%
ATCTTGGACTGTTTAAGTCT+TGG - Chr8:84067382-84067401 MsG0880047277.01.T01:three_prime_UTR 35.0%
ATGTTCCTTGGATTGAACTA+GGG - Chr8:84065502-84065521 MsG0880047277.01.T01:intron 35.0%
ATTTCAATGTCGAGTCGAAT+GGG + Chr8:84065063-84065082 None:intergenic 35.0%
ATTTGAGTAACAAAAGGTGG+AGG + Chr8:84065287-84065306 None:intergenic 35.0%
CAAAGGAAGAGAGAAATTTC+AGG - Chr8:84068101-84068120 MsG0880047277.01.T01:intron 35.0%
CAAATGTAGACACATACATG+AGG + Chr8:84066582-84066601 None:intergenic 35.0%
CAGCACATTTCATTTCTCTA+CGG - Chr8:84066302-84066321 MsG0880047277.01.T01:intron 35.0%
CCGGCATTGTATTATATGAT+TGG - Chr8:84066519-84066538 MsG0880047277.01.T01:intron 35.0%
CTATCATCTGTTGACAGTAA+AGG - Chr8:84067221-84067240 MsG0880047277.01.T01:three_prime_UTR 35.0%
CTTAAACAGTCCAAGATGAT+CGG + Chr8:84067379-84067398 None:intergenic 35.0%
GAAAGCAGATTAAGCAAGAA+GGG - Chr8:84067063-84067082 MsG0880047277.01.T01:three_prime_UTR 35.0%
GCAATTTGAGTAACAAAAGG+TGG + Chr8:84065290-84065309 None:intergenic 35.0%
GTCGCTTGTAATCATATCAT+TGG + Chr8:84066053-84066072 None:intergenic 35.0%
TACGAGGTCATTCATTCTAT+TGG - Chr8:84066879-84066898 MsG0880047277.01.T01:three_prime_UTR 35.0%
TATCAAAGTTGTTCAAGTCC+TGG + Chr8:84066612-84066631 None:intergenic 35.0%
TATCCATGAGCGCTTATATA+AGG + Chr8:84065547-84065566 None:intergenic 35.0%
TATGTTCCTTGGATTGAACT+AGG - Chr8:84065501-84065520 MsG0880047277.01.T01:intron 35.0%
TGAATGCATCAGGAAAAGAA+AGG - Chr8:84068015-84068034 MsG0880047277.01.T01:CDS 35.0%
TTATAGGACCAGTAGTCAAT+TGG - Chr8:84068230-84068249 MsG0880047277.01.T01:five_prime_UTR 35.0%
TTCTGTAGAAAGCAGAAACT+GGG + Chr8:84064926-84064945 None:intergenic 35.0%
TTGGATAGAAGATCCACAAT+GGG + Chr8:84068058-84068077 None:intergenic 35.0%
TTTCTGTAGAAAGCAGAAAC+TGG + Chr8:84064927-84064946 None:intergenic 35.0%
! AGTTGATATCCTTGAAGCAA+AGG - Chr8:84068084-84068103 MsG0880047277.01.T01:intron 35.0%
! CTCTCTCTTTTTATGTTCCT+TGG - Chr8:84065490-84065509 MsG0880047277.01.T01:intron 35.0%
! GAGAGAAATTTCAGGTGAAT+TGG - Chr8:84068109-84068128 MsG0880047277.01.T01:intron 35.0%
! GCGCTTATATAAGGTGTTTA+TGG + Chr8:84065538-84065557 None:intergenic 35.0%
! TAAGGTTGTCAAGGTACTTA+TGG + Chr8:84065850-84065869 None:intergenic 35.0%
!! ACAAATTTGATGATGGTGCA+AGG - Chr8:84067629-84067648 MsG0880047277.01.T01:CDS 35.0%
AAGGATATCAACTTCCTCGT+TGG + Chr8:84068077-84068096 None:intergenic 40.0%
AATCGACGGTCATAGATACA+AGG - Chr8:84065080-84065099 MsG0880047277.01.T01:intron 40.0%
ACACCTTATATAAGCGCTCA+TGG - Chr8:84065541-84065560 MsG0880047277.01.T01:intron 40.0%
ACTTACATCCTGAACTTGAC+AGG + Chr8:84064951-84064970 None:intergenic 40.0%
AGAGAAGATTGTTCCCATCA+AGG - Chr8:84065460-84065479 MsG0880047277.01.T01:intron 40.0%
ATGCTGAACCAATTGACTAC+TGG + Chr8:84068241-84068260 None:intergenic 40.0%
CAGTTTGTAAGTGTTATGGC+TGG - Chr8:84065695-84065714 MsG0880047277.01.T01:intron 40.0%
CATCAGGAAAAGAAAGGAGT+GGG - Chr8:84068021-84068040 MsG0880047277.01.T01:CDS 40.0%
CTGAACTATCTGATTAGGCA+TGG + Chr8:84068146-84068165 None:intergenic 40.0%
CTTACATCCTGAACTTGACA+GGG + Chr8:84064950-84064969 None:intergenic 40.0%
GATATACTTACTCCAACCTC+AGG + Chr8:84066687-84066706 None:intergenic 40.0%
GATTAGGCATGGTTGACTAT+GGG + Chr8:84068135-84068154 None:intergenic 40.0%
GATTTCAATGTCGAGTCGAA+TGG + Chr8:84065064-84065083 None:intergenic 40.0%
GCACATTTCATTTCTCTACG+GGG - Chr8:84066304-84066323 MsG0880047277.01.T01:intron 40.0%
GCTGAATTTCGATCCTATGA+CGG - Chr8:84065419-84065438 MsG0880047277.01.T01:intron 40.0%
GCTGCAAAACAGTAGCATTT+AGG + Chr8:84066829-84066848 None:intergenic 40.0%
GGAGAAGCTACTTGTTTGTT+TGG - Chr8:84067781-84067800 MsG0880047277.01.T01:intron 40.0%
GGATGTATATGAACCCATTG+TGG - Chr8:84068042-84068061 MsG0880047277.01.T01:CDS 40.0%
GTCGTTTAAGCATCTCTCAA+GGG - Chr8:84066363-84066382 MsG0880047277.01.T01:intron 40.0%
GTTGGATAGAAGATCCACAA+TGG + Chr8:84068059-84068078 None:intergenic 40.0%
TCAGATTGTAGTTCAGAGTG+TGG - Chr8:84067029-84067048 MsG0880047277.01.T01:three_prime_UTR 40.0%
TCATCTGTTGACAGTAAAGG+AGG - Chr8:84067224-84067243 MsG0880047277.01.T01:three_prime_UTR 40.0%
TCTGTAGAAAGCAGAAACTG+GGG + Chr8:84064925-84064944 None:intergenic 40.0%
TGATTAGGCATGGTTGACTA+TGG + Chr8:84068136-84068155 None:intergenic 40.0%
TTCTCTCTTCCTTTGCTTCA+AGG + Chr8:84068096-84068115 None:intergenic 40.0%
! TAAGGTGTTTATGGAACAGG+AGG + Chr8:84065529-84065548 None:intergenic 40.0%
! TCCAAATGGGCCTTATATAC+TGG + Chr8:84065721-84065740 None:intergenic 40.0%
! TGTCGTTTAAGCATCTCTCA+AGG - Chr8:84066362-84066381 MsG0880047277.01.T01:intron 40.0%
! TTATGGCTGGCCAGTATATA+AGG - Chr8:84065708-84065727 MsG0880047277.01.T01:intron 40.0%
!!! CTGATTTGCTGCACTTTTGT+TGG - Chr8:84066554-84066573 MsG0880047277.01.T01:intron 40.0%
!!! CTGTTTTGGTTGAATGCATC+AGG - Chr8:84068005-84068024 MsG0880047277.01.T01:CDS 40.0%
AAAAGGGCAGGCTTCTTTGA+TGG + Chr8:84065978-84065997 None:intergenic 45.0%
AAAGCTGTCTCTGATGGCAA+AGG + Chr8:84066860-84066879 None:intergenic 45.0%
AAAGGGCAGGCTTCTTTGAT+GGG + Chr8:84065977-84065996 None:intergenic 45.0%
AAGAACCTTCTGCAGAGACA+TGG - Chr8:84066650-84066669 MsG0880047277.01.T01:three_prime_UTR 45.0%
ACAGAAACCCTGTCAAGTTC+AGG - Chr8:84064940-84064959 MsG0880047277.01.T01:three_prime_UTR 45.0%
AGAACCTTCTGCAGAGACAT+GGG - Chr8:84066651-84066670 MsG0880047277.01.T01:three_prime_UTR 45.0%
AGCCTCAAAATCCAGCTGTA+AGG - Chr8:84067524-84067543 MsG0880047277.01.T01:three_prime_UTR 45.0%
CGACTCGACATTGAAATCGA+CGG - Chr8:84065066-84065085 MsG0880047277.01.T01:intron 45.0%
GAAGGTTCTTGCAACTCTTC+AGG + Chr8:84066640-84066659 None:intergenic 45.0%
GACGGTCAAGATATGTATGC+TGG - Chr8:84065437-84065456 MsG0880047277.01.T01:intron 45.0%
GATACAAGGATGCCTTCACT+TGG - Chr8:84065094-84065113 MsG0880047277.01.T01:intron 45.0%
GCATCAGGAAAAGAAAGGAG+TGG - Chr8:84068020-84068039 MsG0880047277.01.T01:CDS 45.0%
GCCAGTATATAAGGCCCATT+TGG - Chr8:84065717-84065736 MsG0880047277.01.T01:intron 45.0%
TATGGGTAAGATTGCAGCAC+CGG - Chr8:84066500-84066519 MsG0880047277.01.T01:intron 45.0%
TCTGATGGCAAAGGCAATAG+TGG + Chr8:84066851-84066870 None:intergenic 45.0%
TGTGGATCTTCTATCCAACG+AGG - Chr8:84068060-84068079 MsG0880047277.01.T01:intron 45.0%
TGTTCAGTTGTTCGTTCACC+AGG + Chr8:84064830-84064849 None:intergenic 45.0%
! AATGGGTACGAGATTTTCCG+AGG + Chr8:84065046-84065065 None:intergenic 45.0%
! GTGGACTTGGTCAAGCTATT+TGG - Chr8:84067257-84067276 MsG0880047277.01.T01:three_prime_UTR 45.0%
! TGATGGTGCAAGGACTATTG+TGG - Chr8:84067639-84067658 MsG0880047277.01.T01:CDS 45.0%
! TGCCTTACAGCTGGATTTTG+AGG + Chr8:84067529-84067548 None:intergenic 45.0%
!! GATTAATTAATTAATTAATT+TGG - Chr8:84064802-84064821 MsG0880047277.01.T01:three_prime_UTR 5.0%
!! TTAATATTAAGTAATTTATA+AGG - Chr8:84067473-84067492 MsG0880047277.01.T01:three_prime_UTR 5.0%
!! TTAATTAATTAATTAATCTT+TGG + Chr8:84064800-84064819 None:intergenic 5.0%
AAGGGCAGGCTTCTTTGATG+GGG + Chr8:84065976-84065995 None:intergenic 50.0%
AATGCCCATGTCTCTGCAGA+AGG + Chr8:84066658-84066677 None:intergenic 50.0%
ACCAGAAGGGTTCCAAGTGA+AGG + Chr8:84065109-84065128 None:intergenic 50.0%
AGAGAGAGGCAGACCTTGAT+GGG + Chr8:84065476-84065495 None:intergenic 50.0%
CAAAACACACAGGCCAACCT+CGG - Chr8:84065026-84065045 MsG0880047277.01.T01:three_prime_UTR 50.0%
CCATCAGAGACAGCTTTACG+AGG - Chr8:84066863-84066882 MsG0880047277.01.T01:three_prime_UTR 50.0%
CCTCGTAAAGCTGTCTCTGA+TGG + Chr8:84066866-84066885 None:intergenic 50.0%
CTGCTTAACTGCCTTACAGC+TGG + Chr8:84067538-84067557 None:intergenic 50.0%
GAGGACCCTAGTTCAATCCA+AGG + Chr8:84065510-84065529 None:intergenic 50.0%
GCTGTAAGGCAGTTAAGCAG+CGG - Chr8:84067538-84067557 MsG0880047277.01.T01:three_prime_UTR 50.0%
TAAGCAAGAAGGGTCGTCGA+GGG - Chr8:84067073-84067092 MsG0880047277.01.T01:three_prime_UTR 50.0%
TTAAGCAAGAAGGGTCGTCG+AGG - Chr8:84067072-84067091 MsG0880047277.01.T01:three_prime_UTR 50.0%
! GGTACGAGATTTTCCGAGGT+TGG + Chr8:84065042-84065061 None:intergenic 50.0%
AAGCAAGAAGGGTCGTCGAG+GGG - Chr8:84067074-84067093 MsG0880047277.01.T01:three_prime_UTR 55.0%
AGGGCAGGCTTCTTTGATGG+GGG + Chr8:84065975-84065994 None:intergenic 55.0%
GAGAGAGAGGCAGACCTTGA+TGG + Chr8:84065477-84065496 None:intergenic 55.0%
GCCTTCACTTGGAACCCTTC+TGG - Chr8:84065105-84065124 MsG0880047277.01.T01:intron 55.0%
! CTGCAGAGACATGGGCATTG+AGG - Chr8:84066659-84066678 MsG0880047277.01.T01:three_prime_UTR 55.0%
! GGCATTGAGGATCCTGAGGT+TGG - Chr8:84066672-84066691 MsG0880047277.01.T01:three_prime_UTR 55.0%
! GGGCTGACTTCTTTCCAGTC+AGG - Chr8:84067094-84067113 MsG0880047277.01.T01:three_prime_UTR 55.0%
!! CATGGGCATTGAGGATCCTG+AGG - Chr8:84066668-84066687 MsG0880047277.01.T01:three_prime_UTR 55.0%
!! TAAAGGAGGCGCTGTAGCAG+TGG - Chr8:84067238-84067257 MsG0880047277.01.T01:three_prime_UTR 55.0%
! AGGCGCTGTAGCAGTGGACT+TGG - Chr8:84067244-84067263 MsG0880047277.01.T01:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 84064799 84068312 84064799 ID=MsG0880047277.01;Name=MsG0880047277.01
Chr8 mRNA 84064799 84068312 84064799 ID=MsG0880047277.01.T01;Parent=MsG0880047277.01;Name=MsG0880047277.01.T01;_AED=0.28;_eAED=0.28;_QI=116|1|1|1|0.5|0.66|9|1539|113
Chr8 exon 84065996 84066078 84065996 ID=MsG0880047277.01.T01:exon:20948;Parent=MsG0880047277.01.T01
Chr8 exon 84066227 84066263 84066227 ID=MsG0880047277.01.T01:exon:20947;Parent=MsG0880047277.01.T01
Chr8 exon 84066416 84066498 84066416 ID=MsG0880047277.01.T01:exon:20946;Parent=MsG0880047277.01.T01
Chr8 exon 84066607 84067701 84066607 ID=MsG0880047277.01.T01:exon:20945;Parent=MsG0880047277.01.T01
Chr8 exon 84067789 84067883 84067789 ID=MsG0880047277.01.T01:exon:20944;Parent=MsG0880047277.01.T01
Chr8 exon 84067985 84068073 84067985 ID=MsG0880047277.01.T01:exon:20943;Parent=MsG0880047277.01.T01
Chr8 exon 84068147 84068312 84068147 ID=MsG0880047277.01.T01:exon:20942;Parent=MsG0880047277.01.T01
Chr8 five_prime_UTR 84068197 84068312 84068197 ID=MsG0880047277.01.T01:five_prime_utr;Parent=MsG0880047277.01.T01
Chr8 CDS 84068147 84068196 84068147 ID=MsG0880047277.01.T01:cds;Parent=MsG0880047277.01.T01
Chr8 CDS 84067985 84068073 84067985 ID=MsG0880047277.01.T01:cds;Parent=MsG0880047277.01.T01
Chr8 CDS 84067789 84067883 84067789 ID=MsG0880047277.01.T01:cds;Parent=MsG0880047277.01.T01
Chr8 CDS 84067594 84067701 84067594 ID=MsG0880047277.01.T01:cds;Parent=MsG0880047277.01.T01
Chr8 three_prime_UTR 84066607 84067593 84066607 ID=MsG0880047277.01.T01:three_prime_utr;Parent=MsG0880047277.01.T01
Chr8 three_prime_UTR 84066416 84066498 84066416 ID=MsG0880047277.01.T01:three_prime_utr;Parent=MsG0880047277.01.T01
Chr8 three_prime_UTR 84066227 84066263 84066227 ID=MsG0880047277.01.T01:three_prime_utr;Parent=MsG0880047277.01.T01
Chr8 three_prime_UTR 84065996 84066078 84065996 ID=MsG0880047277.01.T01:three_prime_utr;Parent=MsG0880047277.01.T01
Chr8 three_prime_UTR 84065811 84065873 84065811 ID=MsG0880047277.01.T01:three_prime_utr;Parent=MsG0880047277.01.T01
Chr8 three_prime_UTR 84064799 84065084 84064799 ID=MsG0880047277.01.T01:three_prime_utr;Parent=MsG0880047277.01.T01
Chr8 mRNA 84064799 84066528 84064799 ID=MsG0880047277.01.T02;Parent=MsG0880047277.01;Name=MsG0880047277.01.T02;_AED=0.45;_eAED=0.45;_QI=0|0.5|0.66|1|1|1|3|186|91
Chr8 exon 84066416 84066528 84066416 ID=MsG0880047277.01.T02:exon:20951;Parent=MsG0880047277.01.T02
Chr8 CDS 84066416 84066528 84066416 ID=MsG0880047277.01.T02:cds;Parent=MsG0880047277.01.T02
Chr8 CDS 84065811 84065873 84065811 ID=MsG0880047277.01.T02:cds;Parent=MsG0880047277.01.T02
Chr8 CDS 84064985 84065084 84064985 ID=MsG0880047277.01.T02:cds;Parent=MsG0880047277.01.T02
Chr8 three_prime_UTR 84064799 84064984 84064799 ID=MsG0880047277.01.T02:three_prime_utr;Parent=MsG0880047277.01.T02
Gene Sequence

>MsG0880047277.01.T02

ATGTATGTGTCTACATTTGTTAAATTCCAGGACTTGAACAACTTTGATAGTGATCCTGAAGAGTTGCAAGAACCTTCTGCAGAGACATGGGCATTGAGGATCCTGAGGTTGGATAAAGGAGGCGCTGTAGCAGTGGACTTGGTCAAGCTATTTGGTATTAAATCTAGTGTTGTTCGGAAAAGAAAGGAGTGGGATGTATATGAACCCATTGTGGATCTTCTATCCAACGAGGAAGTTGATATCCTTGAAGCAAAGGAAGAGAGAAATTTCAGGTGA

>MsG0880047277.01.T01

ATGAAAACCCCAGTTTCTGCTTTCTACAGAAACCCTGTCAAGTTCAGGATGCCAACCTCGGAAAATCTCGTACCCATTCGACTCGACATTGAAATCGACGGTCATAGATACAAGGATGCCTTCACTTGGAACCCTTCTGATCCTGATTCTGAGATTGTAGTGTTTGCTAAAAGAACTGTTAAAGATTTGAAGCTACCTCCACCTTTTGTTACTCAAATTGCTCAATCCATTCAATCTCAGCTTGCTGAATTTCGATCCTATGACGGTCAAGATATGTATGCTGGAGAGAAGATTGTTCCCATCAAGGTCTGCCTCTCTCTCTTTTTATGTTCCTTGGATTGA
Protein sequence

>MsG0880047277.01.T02

MYVSTFVKFQDLNNFDSDPEELQEPSAETWALRILRLDKGGAVAVDLVKLFGIKSSVVRKRKEWDVYEPIVDLLSNEEVDILEAKEERNFR*

>MsG0880047277.01.T01

MKTPVSAFYRNPVKFRMPTSENLVPIRLDIEIDGHRYKDAFTWNPSDPDSEIVVFAKRTVKDLKLPPPFVTQIAQSIQSQLAEFRSYDGQDMYAGEKIVPIKVCLSLFLCSLD*