Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044910.01.T03 | XP_003628592.1 | 97.143 | 105 | 3 | 0 | 1 | 105 | 343 | 447 | 4.19E-67 | 218 |
| MsG0880044910.01.T04 | XP_003628592.1 | 95.652 | 69 | 3 | 0 | 1 | 69 | 379 | 447 | 8.17E-38 | 140 |
| MsG0880044910.01.T02 | XP_003628592.1 | 93.674 | 411 | 26 | 0 | 1 | 411 | 37 | 447 | 0 | 799 |
| MsG0880044910.01.T01 | XP_003628592.1 | 93.289 | 447 | 30 | 0 | 1 | 447 | 1 | 447 | 0 | 866 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044910.01.T01 | Q9AR19 | 67.179 | 390 | 122 | 4 | 58 | 445 | 181 | 566 | 0 | 567 |
| MsG0880044910.01.T02 | Q9AR19 | 67.179 | 390 | 122 | 4 | 22 | 409 | 181 | 566 | 0 | 566 |
| MsG0880044910.01.T03 | Q9AR19 | 70.874 | 103 | 30 | 0 | 1 | 103 | 464 | 566 | 4.51E-49 | 166 |
| MsG0880044910.01.T04 | Q9AR19 | 71.642 | 67 | 19 | 0 | 1 | 67 | 500 | 566 | 2.53E-28 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044910.01.T01 | G7LEM3 | 93.289 | 447 | 30 | 0 | 1 | 447 | 1 | 447 | 0.0 | 866 |
| MsG0880044910.01.T02 | G7LEM3 | 93.674 | 411 | 26 | 0 | 1 | 411 | 37 | 447 | 0.0 | 799 |
| MsG0880044910.01.T04 | G7LEM3 | 95.652 | 69 | 3 | 0 | 1 | 69 | 379 | 447 | 3.90e-38 | 140 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0880044910.01.T02 | TR | GNAT |
| MsG0880044910.01.T01 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044910.01.T03 | MTR_8g062330 | 97.143 | 105 | 3 | 0 | 1 | 105 | 343 | 447 | 5.08e-71 | 218 |
| MsG0880044910.01.T03 | MTR_7g096040 | 75.238 | 105 | 26 | 0 | 1 | 105 | 438 | 542 | 8.17e-55 | 178 |
| MsG0880044910.01.T03 | MTR_2g018270 | 41.935 | 93 | 51 | 2 | 3 | 93 | 85 | 176 | 1.14e-14 | 68.6 |
| MsG0880044910.01.T03 | MTR_0018s0090 | 38.144 | 97 | 57 | 2 | 3 | 97 | 203 | 298 | 2.39e-14 | 67.8 |
| MsG0880044910.01.T03 | MTR_0018s0090 | 38.144 | 97 | 57 | 2 | 3 | 97 | 203 | 298 | 4.02e-14 | 67.0 |
| MsG0880044910.01.T03 | MTR_8g070660 | 38.000 | 100 | 58 | 2 | 3 | 98 | 99 | 198 | 1.11e-13 | 65.1 |
| MsG0880044910.01.T03 | MTR_8g070660 | 38.000 | 100 | 58 | 2 | 3 | 98 | 99 | 198 | 2.91e-13 | 64.3 |
| MsG0880044910.01.T03 | MTR_3g075040 | 36.264 | 91 | 55 | 2 | 3 | 91 | 212 | 301 | 1.14e-12 | 62.8 |
| MsG0880044910.01.T03 | MTR_3g075040 | 36.264 | 91 | 55 | 2 | 3 | 91 | 212 | 301 | 1.47e-12 | 62.4 |
| MsG0880044910.01.T03 | MTR_8g005100 | 41.304 | 92 | 49 | 3 | 17 | 105 | 98 | 187 | 5.04e-12 | 60.1 |
| MsG0880044910.01.T03 | MTR_3g115560 | 40.845 | 71 | 41 | 1 | 17 | 87 | 30 | 99 | 7.92e-12 | 60.5 |
| MsG0880044910.01.T03 | MTR_3g115560 | 40.845 | 71 | 41 | 1 | 17 | 87 | 144 | 213 | 9.06e-12 | 60.5 |
| MsG0880044910.01.T04 | MTR_8g062330 | 95.652 | 69 | 3 | 0 | 1 | 69 | 379 | 447 | 9.90e-42 | 140 |
| MsG0880044910.01.T04 | MTR_7g096040 | 75.362 | 69 | 17 | 0 | 1 | 69 | 474 | 542 | 2.49e-32 | 116 |
| MsG0880044910.01.T02 | MTR_8g062330 | 93.674 | 411 | 26 | 0 | 1 | 411 | 37 | 447 | 0.0 | 799 |
| MsG0880044910.01.T02 | MTR_7g096040 | 75.802 | 405 | 92 | 5 | 8 | 411 | 143 | 542 | 0.0 | 645 |
| MsG0880044910.01.T02 | MTR_2g018270 | 41.935 | 93 | 51 | 2 | 309 | 399 | 85 | 176 | 1.21e-12 | 69.7 |
| MsG0880044910.01.T01 | MTR_8g062330 | 93.289 | 447 | 30 | 0 | 1 | 447 | 1 | 447 | 0.0 | 866 |
| MsG0880044910.01.T01 | MTR_7g096040 | 75.802 | 405 | 92 | 5 | 44 | 447 | 143 | 542 | 0.0 | 646 |
| MsG0880044910.01.T01 | MTR_2g018270 | 41.935 | 93 | 51 | 2 | 345 | 435 | 85 | 176 | 1.14e-12 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044910.01.T03 | AT3G54610 | 70.874 | 103 | 30 | 0 | 1 | 103 | 464 | 566 | 4.60e-50 | 166 |
| MsG0880044910.01.T03 | AT5G63320 | 36.957 | 92 | 55 | 2 | 3 | 92 | 167 | 257 | 3.63e-15 | 70.1 |
| MsG0880044910.01.T03 | AT5G63320 | 36.957 | 92 | 55 | 2 | 3 | 92 | 167 | 257 | 3.63e-15 | 70.1 |
| MsG0880044910.01.T03 | AT5G63320 | 36.957 | 92 | 55 | 2 | 3 | 92 | 167 | 257 | 1.74e-14 | 68.2 |
| MsG0880044910.01.T03 | AT2G34900 | 38.710 | 93 | 54 | 2 | 3 | 93 | 6 | 97 | 1.93e-14 | 67.0 |
| MsG0880044910.01.T03 | AT2G34900 | 38.710 | 93 | 54 | 2 | 3 | 93 | 116 | 207 | 4.48e-14 | 66.6 |
| MsG0880044910.01.T03 | AT3G01770 | 38.318 | 107 | 61 | 3 | 3 | 105 | 135 | 240 | 6.53e-13 | 63.5 |
| MsG0880044910.01.T03 | AT1G76380 | 46.269 | 67 | 35 | 1 | 17 | 83 | 165 | 230 | 1.51e-12 | 62.4 |
| MsG0880044910.01.T03 | AT1G76380 | 46.269 | 67 | 35 | 1 | 17 | 83 | 166 | 231 | 1.55e-12 | 62.4 |
| MsG0880044910.01.T03 | AT1G76380 | 46.269 | 67 | 35 | 1 | 17 | 83 | 165 | 230 | 1.71e-12 | 62.4 |
| MsG0880044910.01.T03 | AT3G01770 | 38.318 | 107 | 61 | 3 | 3 | 105 | 135 | 240 | 2.26e-12 | 62.0 |
| MsG0880044910.01.T03 | AT3G27260 | 36.082 | 97 | 59 | 2 | 3 | 97 | 182 | 277 | 7.35e-12 | 60.5 |
| MsG0880044910.01.T03 | AT3G27260 | 36.082 | 97 | 59 | 2 | 3 | 97 | 182 | 277 | 7.94e-12 | 60.5 |
| MsG0880044910.01.T03 | AT3G27260 | 36.082 | 97 | 59 | 2 | 3 | 97 | 133 | 228 | 8.88e-12 | 60.5 |
| MsG0880044910.01.T03 | AT3G27260 | 36.082 | 97 | 59 | 2 | 3 | 97 | 182 | 277 | 1.02e-11 | 60.1 |
| MsG0880044910.01.T03 | AT1G20670 | 42.254 | 71 | 40 | 1 | 17 | 87 | 196 | 265 | 1.44e-11 | 59.7 |
| MsG0880044910.01.T04 | AT3G54610 | 71.642 | 67 | 19 | 0 | 1 | 67 | 500 | 566 | 2.58e-29 | 108 |
| MsG0880044910.01.T02 | AT3G54610 | 67.179 | 390 | 122 | 4 | 22 | 409 | 181 | 566 | 0.0 | 566 |
| MsG0880044910.01.T02 | AT5G63320 | 36.957 | 92 | 55 | 2 | 309 | 398 | 167 | 257 | 1.09e-12 | 70.1 |
| MsG0880044910.01.T02 | AT5G63320 | 36.957 | 92 | 55 | 2 | 309 | 398 | 167 | 257 | 1.09e-12 | 70.1 |
| MsG0880044910.01.T02 | AT2G34900 | 37.736 | 106 | 58 | 4 | 301 | 399 | 103 | 207 | 1.52e-12 | 68.9 |
| MsG0880044910.01.T02 | AT2G34900 | 38.710 | 93 | 54 | 2 | 309 | 399 | 6 | 97 | 1.62e-12 | 67.8 |
| MsG0880044910.01.T02 | AT5G63320 | 36.957 | 92 | 55 | 2 | 309 | 398 | 167 | 257 | 2.62e-12 | 69.3 |
| MsG0880044910.01.T01 | AT3G54610 | 67.179 | 390 | 122 | 4 | 58 | 445 | 181 | 566 | 0.0 | 567 |
| MsG0880044910.01.T01 | AT2G34900 | 37.736 | 106 | 58 | 4 | 337 | 435 | 103 | 207 | 1.17e-12 | 69.7 |
| MsG0880044910.01.T01 | AT5G63320 | 36.957 | 92 | 55 | 2 | 345 | 434 | 167 | 257 | 1.22e-12 | 70.1 |
| MsG0880044910.01.T01 | AT5G63320 | 36.957 | 92 | 55 | 2 | 345 | 434 | 167 | 257 | 1.22e-12 | 70.1 |
| MsG0880044910.01.T01 | AT2G34900 | 38.710 | 93 | 54 | 2 | 345 | 435 | 6 | 97 | 1.29e-12 | 68.6 |
| MsG0880044910.01.T01 | AT5G63320 | 36.957 | 92 | 55 | 2 | 345 | 434 | 167 | 257 | 3.08e-12 | 69.3 |
Find 105 sgRNAs with CRISPR-Local
Find 113 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAAGTCTATAATGGTTATA+AGG | 0.249320 | 8:+51317204 | MsG0880044910.01.T01:CDS |
| TGTAGCACATTTGTAATATA+TGG | 0.254796 | 8:-51318044 | None:intergenic |
| GTTGATGCACTGAGTGTTCC+TGG | 0.279165 | 8:+51317875 | MsG0880044910.01.T01:CDS |
| TTATAACCATTATAGACTTA+TGG | 0.281569 | 8:-51317202 | None:intergenic |
| AGTGATGATCAACAAGTAAA+AGG | 0.317455 | 8:+51317308 | MsG0880044910.01.T01:CDS |
| CGCGAATGACCGTACATATC+AGG | 0.322015 | 8:-51317731 | None:intergenic |
| TGCATTCTCAAACATTCTTC+TGG | 0.326767 | 8:-51317999 | None:intergenic |
| CTCATTGTCTTTAGATCCAT+TGG | 0.327319 | 8:-51317920 | None:intergenic |
| CGAAGGCCGAAGGGCCAGTC+TGG | 0.327450 | 8:-51316797 | None:intergenic |
| ATGTACTCCTTGGGCATATT+GGG | 0.349906 | 8:-51317155 | None:intergenic |
| AACAGTTCAGCCGCTACTTC+CGG | 0.350067 | 8:+51316921 | MsG0880044910.01.T01:CDS |
| AATGTACTCCTTGGGCATAT+TGG | 0.350870 | 8:-51317156 | None:intergenic |
| AAATCAGTATAAGGAAGCTT+TGG | 0.358698 | 8:-51317536 | None:intergenic |
| TCCTTGAATGGCCAAGCATC+AGG | 0.363142 | 8:-51317848 | None:intergenic |
| GGGCATATTGGGAAGTTGTT+TGG | 0.371176 | 8:-51317144 | None:intergenic |
| ATGTCATCAACAATTCTATT+AGG | 0.371443 | 8:-51317680 | None:intergenic |
| GTACATTGTCCGACTTGTTA+TGG | 0.381254 | 8:+51317172 | MsG0880044910.01.T01:CDS |
| GCAAACCTTTGATTTGCATA+TGG | 0.383325 | 8:-51317263 | None:intergenic |
| CGGTTCTGTCCCCGACTTTG+TGG | 0.384446 | 8:+51316941 | MsG0880044910.01.T01:CDS |
| CCCTGATGCTTGGCCATTCA+AGG | 0.396001 | 8:+51317847 | MsG0880044910.01.T01:CDS |
| ATAGTTGATAAATCAGTATA+AGG | 0.397900 | 8:-51317545 | None:intergenic |
| CTCAAGAAAGAGGAAGAATC+TGG | 0.399688 | 8:+51317041 | MsG0880044910.01.T01:CDS |
| TCTCTCCAAGTGAATCTCTT+TGG | 0.400262 | 8:-51317450 | None:intergenic |
| TAAGTCTATAATGGTTATAA+GGG | 0.403247 | 8:+51317205 | MsG0880044910.01.T01:CDS |
| TGTCCCCGACTTTGTGGTTA+AGG | 0.412892 | 8:+51316947 | MsG0880044910.01.T01:CDS |
| TGTTGATGACATCCCTGGTT+TGG | 0.413040 | 8:+51317691 | MsG0880044910.01.T01:CDS |
| TAGGAACCATAAGTCTATAA+TGG | 0.415215 | 8:+51317196 | MsG0880044910.01.T01:CDS |
| GATCAACAAGTAAAAGGTTA+TGG | 0.418177 | 8:+51317314 | MsG0880044910.01.T01:CDS |
| TCTTCCTTAACCACAAAGTC+GGG | 0.423046 | 8:-51316951 | None:intergenic |
| TTTCTTGAGATTCTCTTCCC+TGG | 0.429530 | 8:-51317027 | None:intergenic |
| GGCTGAACTGTTCACAGAAC+TGG | 0.430611 | 8:-51316910 | None:intergenic |
| GTTTATGCGTTCAATTCTAA+AGG | 0.430657 | 8:+51317811 | MsG0880044910.01.T01:CDS |
| TAAGAGGTGGTTACAGTGCC+AGG | 0.431578 | 8:+51317009 | MsG0880044910.01.T01:CDS |
| CAGATATGTGTTTGCGCTTG+TGG | 0.433930 | 8:-51316833 | None:intergenic |
| TGTCACAATATTGTTTATGC+TGG | 0.434218 | 8:+51317620 | MsG0880044910.01.T01:CDS |
| AGGGAGCATAATCGCGTTGT+TGG | 0.436622 | 8:+51317224 | MsG0880044910.01.T01:CDS |
| CACAAAGTCGGGGACAGAAC+CGG | 0.441013 | 8:-51316940 | None:intergenic |
| TATGCTGACAACAATGCTAT+TGG | 0.442748 | 8:+51317404 | MsG0880044910.01.T01:CDS |
| TCGATGGGAAGGTTATATAA+AGG | 0.445441 | 8:+51317475 | MsG0880044910.01.T01:CDS |
| AATCAGTATAAGGAAGCTTT+GGG | 0.448274 | 8:-51317535 | None:intergenic |
| CAGTATGCACGTGATGTAGA+TGG | 0.451140 | 8:+51317362 | MsG0880044910.01.T01:CDS |
| GTGACAACGCTCCTCCAGTC+TGG | 0.469276 | 8:+51318094 | MsG0880044910.01.T01:CDS |
| CTCAGTGAATATATTTGTTG+CGG | 0.475945 | 8:-51316976 | None:intergenic |
| GGTTATATAAAGGATTATGA+TGG | 0.483037 | 8:+51317485 | MsG0880044910.01.T01:CDS |
| GGAACACTCAGTGCATCAAC+AGG | 0.490459 | 8:-51317872 | None:intergenic |
| GCATCAACAGGCTCCTTGAA+TGG | 0.493921 | 8:-51317860 | None:intergenic |
| ATGCTATCAACGAAACCATA+TGG | 0.498728 | 8:-51317106 | None:intergenic |
| ACTGAGAAATTGCAGATAAG+AGG | 0.506717 | 8:+51316993 | MsG0880044910.01.T01:CDS |
| CATCCAGCCTCTTCCAAACC+AGG | 0.507643 | 8:-51317704 | None:intergenic |
| TGACGGTGTTGATGACCATA+TGG | 0.509355 | 8:+51317091 | MsG0880044910.01.T01:CDS |
| GAGCATAATCGCGTTGTTGG+AGG | 0.514705 | 8:+51317227 | MsG0880044910.01.T01:CDS |
| AAGTCGGACAATGTACTCCT+TGG | 0.515077 | 8:-51317165 | None:intergenic |
| CTGGTTTGGAAGAGGCTGGA+TGG | 0.515406 | 8:+51317705 | MsG0880044910.01.T01:CDS |
| AAAGACAATGAGTGAGAGGC+TGG | 0.516667 | 8:+51317931 | MsG0880044910.01.T01:CDS |
| AGAATTGTTGATGACATCCC+TGG | 0.521290 | 8:+51317686 | MsG0880044910.01.T01:CDS |
| TATGATCCGTCGGCAAAGAC+AGG | 0.524541 | 8:+51317565 | MsG0880044910.01.T01:CDS |
| CTGATATGTACGGTCATTCG+CGG | 0.528433 | 8:+51317732 | MsG0880044910.01.T01:CDS |
| GTTATATAAAGGATTATGAT+GGG | 0.531624 | 8:+51317486 | MsG0880044910.01.T01:CDS |
| GAAGAAGTGGCGAAGGCCGA+AGG | 0.533161 | 8:-51316807 | None:intergenic |
| TTTCAGAAGAGATCAGAAGC+TGG | 0.533279 | 8:+51317650 | MsG0880044910.01.T01:CDS |
| GGAATCCCAGACTGGCCCTT+CGG | 0.533805 | 8:+51316791 | MsG0880044910.01.T01:CDS |
| GGAAGAGAATCTCAAGAAAG+AGG | 0.533991 | 8:+51317031 | MsG0880044910.01.T01:CDS |
| AGTCGGACAATGTACTCCTT+GGG | 0.536912 | 8:-51317164 | None:intergenic |
| TGCATGACCACCCTGATGCT+TGG | 0.542705 | 8:+51317837 | MsG0880044910.01.T01:CDS |
| ATCCCTGGTTTGGAAGAGGC+TGG | 0.545010 | 8:+51317701 | MsG0880044910.01.T01:CDS |
| GAATCGCGAGAAGGGTGGAA+TGG | 0.550392 | 8:-51316870 | None:intergenic |
| TTCTTCCTTAACCACAAAGT+CGG | 0.550744 | 8:-51316952 | None:intergenic |
| TCGAAGAATCGCGAGAAGGG+TGG | 0.553248 | 8:-51316875 | None:intergenic |
| GCATCGAAGAATCGCGAGAA+GGG | 0.558649 | 8:-51316878 | None:intergenic |
| TGACATCCCTGGTTTGGAAG+AGG | 0.563057 | 8:+51317697 | MsG0880044910.01.T01:CDS |
| TGCATCGAAGAATCGCGAGA+AGG | 0.566382 | 8:-51316879 | None:intergenic |
| GAAGGCCGAAGGGCCAGTCT+GGG | 0.566510 | 8:-51316796 | None:intergenic |
| TATGTGTTTGCGCTTGTGGA+TGG | 0.568768 | 8:-51316829 | None:intergenic |
| CCTAAGATGGAATCCCAGAC+TGG | 0.573043 | 8:+51316783 | None:intergenic |
| TATATAAAGGATTATGATGG+GGG | 0.574524 | 8:+51317488 | MsG0880044910.01.T01:CDS |
| ATCGTCCATATGCAAATCAA+AGG | 0.576246 | 8:+51317258 | MsG0880044910.01.T01:CDS |
| TCACTTGGAGAGAGATCGAT+GGG | 0.581124 | 8:+51317460 | MsG0880044910.01.T01:CDS |
| TTTGAATGTGTGTGCAATGA+CGG | 0.586373 | 8:+51317074 | MsG0880044910.01.T01:CDS |
| AAGAGGTGGTTACAGTGCCA+GGG | 0.587476 | 8:+51317010 | MsG0880044910.01.T01:CDS |
| ATTGGCTATTTCATCAAACA+AGG | 0.588299 | 8:+51317422 | MsG0880044910.01.T01:CDS |
| ATTTACCAAAGAGATTCACT+TGG | 0.590454 | 8:+51317445 | MsG0880044910.01.T01:CDS |
| TTCACTTGGAGAGAGATCGA+TGG | 0.592040 | 8:+51317459 | MsG0880044910.01.T01:CDS |
| ATAGTGCCTGTCTTTGCCGA+CGG | 0.594963 | 8:-51317571 | None:intergenic |
| GGATGGACTCCTGATATGTA+CGG | 0.597833 | 8:+51317722 | MsG0880044910.01.T01:CDS |
| TTTATGATTTCATAATAACC+AGG | 0.609225 | 8:-51317893 | None:intergenic |
| CCTTGAATGGCCAAGCATCA+GGG | 0.611793 | 8:-51317847 | None:intergenic |
| GTGGATGGAAGAAGTGGCGA+AGG | 0.613879 | 8:-51316814 | None:intergenic |
| AAGAAGTGGCGAAGGCCGAA+GGG | 0.614868 | 8:-51316806 | None:intergenic |
| ATCTAAAGACAATGAGTGAG+AGG | 0.615052 | 8:+51317927 | MsG0880044910.01.T01:CDS |
| GGGGACAGAACCGGAAGTAG+CGG | 0.615590 | 8:-51316931 | None:intergenic |
| TTGTCCGACTTGTTATGGAT+AGG | 0.616928 | 8:+51317177 | MsG0880044910.01.T01:CDS |
| TTGGAGAGAGATCGATGGGA+AGG | 0.618097 | 8:+51317464 | MsG0880044910.01.T01:CDS |
| GCGCTTGTGGATGGAAGAAG+TGG | 0.619030 | 8:-51316820 | None:intergenic |
| TCGCGAGAAGGGTGGAATGG+AGG | 0.623596 | 8:-51316867 | None:intergenic |
| CACATTTGAGATGTTTCTCG+CGG | 0.625722 | 8:+51317973 | MsG0880044910.01.T01:CDS |
| GAGAAATTGCAGATAAGAGG+TGG | 0.625928 | 8:+51316996 | MsG0880044910.01.T01:CDS |
| CAGAAGAGATCAGAAGCTGG+AGG | 0.631156 | 8:+51317653 | MsG0880044910.01.T01:CDS |
| TTATATAAAGGATTATGATG+GGG | 0.635609 | 8:+51317487 | MsG0880044910.01.T01:CDS |
| ATTTATCAACTATGATCCGT+CGG | 0.639900 | 8:+51317555 | MsG0880044910.01.T01:CDS |
| ATCCAGCCTCTTCCAAACCA+GGG | 0.651977 | 8:-51317703 | None:intergenic |
| ACAACTTCCCAATATGCCCA+AGG | 0.659674 | 8:+51317148 | MsG0880044910.01.T01:CDS |
| CTTCCTTAACCACAAAGTCG+GGG | 0.661782 | 8:-51316950 | None:intergenic |
| GGTTCCTATCCATAACAAGT+CGG | 0.663748 | 8:-51317181 | None:intergenic |
| CAAGCGCAAACACATATCTG+AGG | 0.695714 | 8:+51316836 | MsG0880044910.01.T01:CDS |
| TGAATGGCCAAGCATCAGGG+TGG | 0.741876 | 8:-51317844 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAGTCTATAATGGTTATA+AGG | + | Chr8:51317204-51317223 | MsG0880044910.01.T01:CDS | 20.0% |
| !! | GTTATATAAAGGATTATGAT+GGG | + | Chr8:51317486-51317505 | MsG0880044910.01.T01:CDS | 20.0% |
| !! | TAAGTCTATAATGGTTATAA+GGG | + | Chr8:51317205-51317224 | MsG0880044910.01.T01:CDS | 20.0% |
| !! | TGAAATCATAAAAAATCCAA+TGG | + | Chr8:51317904-51317923 | MsG0880044910.01.T01:CDS | 20.0% |
| !! | TTATAACCATTATAGACTTA+TGG | - | Chr8:51317205-51317224 | None:intergenic | 20.0% |
| !! | TTATATAAAGGATTATGATG+GGG | + | Chr8:51317487-51317506 | MsG0880044910.01.T01:CDS | 20.0% |
| !! | TTTATGATTTCATAATAACC+AGG | - | Chr8:51317896-51317915 | None:intergenic | 20.0% |
| !!! | ATAGTTGATAAATCAGTATA+AGG | - | Chr8:51317548-51317567 | None:intergenic | 20.0% |
| !!! | CATTTGTAATATATGGTTTT+TGG | - | Chr8:51318040-51318059 | None:intergenic | 20.0% |
| !!! | TAAACTCAGTTAAATGTTTT+AGG | - | Chr8:51317796-51317815 | None:intergenic | 20.0% |
| ! | ATGTCATCAACAATTCTATT+AGG | - | Chr8:51317683-51317702 | None:intergenic | 25.0% |
| ! | GGTTATATAAAGGATTATGA+TGG | + | Chr8:51317485-51317504 | MsG0880044910.01.T01:CDS | 25.0% |
| ! | TATATAAAGGATTATGATGG+GGG | + | Chr8:51317488-51317507 | MsG0880044910.01.T01:CDS | 25.0% |
| ! | TGTAGCACATTTGTAATATA+TGG | - | Chr8:51318047-51318066 | None:intergenic | 25.0% |
| !!! | TATGGTTTTTGGAGAATTAT+AGG | - | Chr8:51318029-51318048 | None:intergenic | 25.0% |
| AAATCAGTATAAGGAAGCTT+TGG | - | Chr8:51317539-51317558 | None:intergenic | 30.0% | |
| AATCAGTATAAGGAAGCTTT+GGG | - | Chr8:51317538-51317557 | None:intergenic | 30.0% | |
| AGTGATGATCAACAAGTAAA+AGG | + | Chr8:51317308-51317327 | MsG0880044910.01.T01:CDS | 30.0% | |
| ATTTACCAAAGAGATTCACT+TGG | + | Chr8:51317445-51317464 | MsG0880044910.01.T01:CDS | 30.0% | |
| ATTTATCAACTATGATCCGT+CGG | + | Chr8:51317555-51317574 | MsG0880044910.01.T01:CDS | 30.0% | |
| GATCAACAAGTAAAAGGTTA+TGG | + | Chr8:51317314-51317333 | MsG0880044910.01.T01:CDS | 30.0% | |
| GTTTATGCGTTCAATTCTAA+AGG | + | Chr8:51317811-51317830 | MsG0880044910.01.T01:CDS | 30.0% | |
| TAGGAACCATAAGTCTATAA+TGG | + | Chr8:51317196-51317215 | MsG0880044910.01.T01:CDS | 30.0% | |
| TGTCACAATATTGTTTATGC+TGG | + | Chr8:51317620-51317639 | MsG0880044910.01.T01:CDS | 30.0% | |
| ! | ATTGGCTATTTCATCAAACA+AGG | + | Chr8:51317422-51317441 | MsG0880044910.01.T01:CDS | 30.0% |
| ! | CTCAGTGAATATATTTGTTG+CGG | - | Chr8:51316979-51316998 | None:intergenic | 30.0% |
| !!! | AATGTTTTAGGTTTGTAGCA+TGG | - | Chr8:51317784-51317803 | None:intergenic | 30.0% |
| ACTGAGAAATTGCAGATAAG+AGG | + | Chr8:51316993-51317012 | MsG0880044910.01.T01:CDS | 35.0% | |
| ATCGTCCATATGCAAATCAA+AGG | + | Chr8:51317258-51317277 | MsG0880044910.01.T01:CDS | 35.0% | |
| ATCTAAAGACAATGAGTGAG+AGG | + | Chr8:51317927-51317946 | MsG0880044910.01.T01:CDS | 35.0% | |
| ATGCTATCAACGAAACCATA+TGG | - | Chr8:51317109-51317128 | None:intergenic | 35.0% | |
| CTCATTGTCTTTAGATCCAT+TGG | - | Chr8:51317923-51317942 | None:intergenic | 35.0% | |
| TATGCTGACAACAATGCTAT+TGG | + | Chr8:51317404-51317423 | MsG0880044910.01.T01:CDS | 35.0% | |
| TCGATGGGAAGGTTATATAA+AGG | + | Chr8:51317475-51317494 | MsG0880044910.01.T01:CDS | 35.0% | |
| TGCATTCTCAAACATTCTTC+TGG | - | Chr8:51318002-51318021 | None:intergenic | 35.0% | |
| TTCTTCCTTAACCACAAAGT+CGG | - | Chr8:51316955-51316974 | None:intergenic | 35.0% | |
| TTTGAATGTGTGTGCAATGA+CGG | + | Chr8:51317074-51317093 | MsG0880044910.01.T01:CDS | 35.0% | |
| !! | GCAAACCTTTGATTTGCATA+TGG | - | Chr8:51317266-51317285 | None:intergenic | 35.0% |
| AATGTACTCCTTGGGCATAT+TGG | - | Chr8:51317159-51317178 | None:intergenic | 40.0% | |
| ATGTACTCCTTGGGCATATT+GGG | - | Chr8:51317158-51317177 | None:intergenic | 40.0% | |
| CTCAAGAAAGAGGAAGAATC+TGG | + | Chr8:51317041-51317060 | MsG0880044910.01.T01:CDS | 40.0% | |
| GAGAAATTGCAGATAAGAGG+TGG | + | Chr8:51316996-51317015 | MsG0880044910.01.T01:CDS | 40.0% | |
| GGAAGAGAATCTCAAGAAAG+AGG | + | Chr8:51317031-51317050 | MsG0880044910.01.T01:CDS | 40.0% | |
| GGTTCCTATCCATAACAAGT+CGG | - | Chr8:51317184-51317203 | None:intergenic | 40.0% | |
| TCTCTCCAAGTGAATCTCTT+TGG | - | Chr8:51317453-51317472 | None:intergenic | 40.0% | |
| TCTTCCTTAACCACAAAGTC+GGG | - | Chr8:51316954-51316973 | None:intergenic | 40.0% | |
| TTTCAGAAGAGATCAGAAGC+TGG | + | Chr8:51317650-51317669 | MsG0880044910.01.T01:CDS | 40.0% | |
| TTTCTTGAGATTCTCTTCCC+TGG | - | Chr8:51317030-51317049 | None:intergenic | 40.0% | |
| ! | CACATTTGAGATGTTTCTCG+CGG | + | Chr8:51317973-51317992 | MsG0880044910.01.T01:CDS | 40.0% |
| !! | AGAATTGTTGATGACATCCC+TGG | + | Chr8:51317686-51317705 | MsG0880044910.01.T01:CDS | 40.0% |
| !! | GTACATTGTCCGACTTGTTA+TGG | + | Chr8:51317172-51317191 | MsG0880044910.01.T01:CDS | 40.0% |
| !! | TTGTCCGACTTGTTATGGAT+AGG | + | Chr8:51317177-51317196 | MsG0880044910.01.T01:CDS | 40.0% |
| !!! | ACTCAATTTTGAAGCCAGAC+TGG | - | Chr8:51318111-51318130 | None:intergenic | 40.0% |
| !!! | TCGCGGTTTAGAGTTTTAGA+TGG | + | Chr8:51317749-51317768 | MsG0880044910.01.T01:CDS | 40.0% |
| AAAGACAATGAGTGAGAGGC+TGG | + | Chr8:51317931-51317950 | MsG0880044910.01.T01:CDS | 45.0% | |
| AAGTCGGACAATGTACTCCT+TGG | - | Chr8:51317168-51317187 | None:intergenic | 45.0% | |
| ACAACTTCCCAATATGCCCA+AGG | + | Chr8:51317148-51317167 | MsG0880044910.01.T01:CDS | 45.0% | |
| AGTCGGACAATGTACTCCTT+GGG | - | Chr8:51317167-51317186 | None:intergenic | 45.0% | |
| CAAGCGCAAACACATATCTG+AGG | + | Chr8:51316836-51316855 | MsG0880044910.01.T01:CDS | 45.0% | |
| CAGATATGTGTTTGCGCTTG+TGG | - | Chr8:51316836-51316855 | None:intergenic | 45.0% | |
| CAGTATGCACGTGATGTAGA+TGG | + | Chr8:51317362-51317381 | MsG0880044910.01.T01:CDS | 45.0% | |
| CTGATATGTACGGTCATTCG+CGG | + | Chr8:51317732-51317751 | MsG0880044910.01.T01:CDS | 45.0% | |
| CTTCCTTAACCACAAAGTCG+GGG | - | Chr8:51316953-51316972 | None:intergenic | 45.0% | |
| GGGCATATTGGGAAGTTGTT+TGG | - | Chr8:51317147-51317166 | None:intergenic | 45.0% | |
| TATGTGTTTGCGCTTGTGGA+TGG | - | Chr8:51316832-51316851 | None:intergenic | 45.0% | |
| TCACTTGGAGAGAGATCGAT+GGG | + | Chr8:51317460-51317479 | MsG0880044910.01.T01:CDS | 45.0% | |
| TTCACTTGGAGAGAGATCGA+TGG | + | Chr8:51317459-51317478 | MsG0880044910.01.T01:CDS | 45.0% | |
| ! | GGATGGACTCCTGATATGTA+CGG | + | Chr8:51317722-51317741 | MsG0880044910.01.T01:CDS | 45.0% |
| ! | TTATGATGGGGGAACTCTCA+TGG | + | Chr8:51317499-51317518 | MsG0880044910.01.T01:CDS | 45.0% |
| !! | TGACGGTGTTGATGACCATA+TGG | + | Chr8:51317091-51317110 | MsG0880044910.01.T01:CDS | 45.0% |
| !! | TGTTGATGACATCCCTGGTT+TGG | + | Chr8:51317691-51317710 | MsG0880044910.01.T01:CDS | 45.0% |
| !!! | CAATTTTGAAGCCAGACTGG+AGG | - | Chr8:51318108-51318127 | None:intergenic | 45.0% |
| AACAGTTCAGCCGCTACTTC+CGG | + | Chr8:51316921-51316940 | MsG0880044910.01.T01:CDS | 50.0% | |
| AAGAGGTGGTTACAGTGCCA+GGG | + | Chr8:51317010-51317029 | MsG0880044910.01.T01:CDS | 50.0% | |
| ATAGTGCCTGTCTTTGCCGA+CGG | - | Chr8:51317574-51317593 | None:intergenic | 50.0% | |
| ATCCAGCCTCTTCCAAACCA+GGG | - | Chr8:51317706-51317725 | None:intergenic | 50.0% | |
| CAGAAGAGATCAGAAGCTGG+AGG | + | Chr8:51317653-51317672 | MsG0880044910.01.T01:CDS | 50.0% | |
| CCTTGAATGGCCAAGCATCA+GGG | - | Chr8:51317850-51317869 | None:intergenic | 50.0% | |
| CGCGAATGACCGTACATATC+AGG | - | Chr8:51317734-51317753 | None:intergenic | 50.0% | |
| GAGCATAATCGCGTTGTTGG+AGG | + | Chr8:51317227-51317246 | MsG0880044910.01.T01:CDS | 50.0% | |
| GCATCAACAGGCTCCTTGAA+TGG | - | Chr8:51317863-51317882 | None:intergenic | 50.0% | |
| GCATCGAAGAATCGCGAGAA+GGG | - | Chr8:51316881-51316900 | None:intergenic | 50.0% | |
| GGAACACTCAGTGCATCAAC+AGG | - | Chr8:51317875-51317894 | None:intergenic | 50.0% | |
| GGCTGAACTGTTCACAGAAC+TGG | - | Chr8:51316913-51316932 | None:intergenic | 50.0% | |
| GTTGATGCACTGAGTGTTCC+TGG | + | Chr8:51317875-51317894 | MsG0880044910.01.T01:CDS | 50.0% | |
| TAAGAGGTGGTTACAGTGCC+AGG | + | Chr8:51317009-51317028 | MsG0880044910.01.T01:CDS | 50.0% | |
| TATGATCCGTCGGCAAAGAC+AGG | + | Chr8:51317565-51317584 | MsG0880044910.01.T01:CDS | 50.0% | |
| TCCTTGAATGGCCAAGCATC+AGG | - | Chr8:51317851-51317870 | None:intergenic | 50.0% | |
| TGCATCGAAGAATCGCGAGA+AGG | - | Chr8:51316882-51316901 | None:intergenic | 50.0% | |
| TGTCCCCGACTTTGTGGTTA+AGG | + | Chr8:51316947-51316966 | MsG0880044910.01.T01:CDS | 50.0% | |
| TTGGAGAGAGATCGATGGGA+AGG | + | Chr8:51317464-51317483 | MsG0880044910.01.T01:CDS | 50.0% | |
| ! | AGGGAGCATAATCGCGTTGT+TGG | + | Chr8:51317224-51317243 | MsG0880044910.01.T01:CDS | 50.0% |
| !! | TGACATCCCTGGTTTGGAAG+AGG | + | Chr8:51317697-51317716 | MsG0880044910.01.T01:CDS | 50.0% |
| AAGAAGTGGCGAAGGCCGAA+GGG | - | Chr8:51316809-51316828 | None:intergenic | 55.0% | |
| CACAAAGTCGGGGACAGAAC+CGG | - | Chr8:51316943-51316962 | None:intergenic | 55.0% | |
| CATCCAGCCTCTTCCAAACC+AGG | - | Chr8:51317707-51317726 | None:intergenic | 55.0% | |
| CCCTGATGCTTGGCCATTCA+AGG | + | Chr8:51317847-51317866 | MsG0880044910.01.T01:CDS | 55.0% | |
| GAATCGCGAGAAGGGTGGAA+TGG | - | Chr8:51316873-51316892 | None:intergenic | 55.0% | |
| GCGCTTGTGGATGGAAGAAG+TGG | - | Chr8:51316823-51316842 | None:intergenic | 55.0% | |
| GTGGATGGAAGAAGTGGCGA+AGG | - | Chr8:51316817-51316836 | None:intergenic | 55.0% | |
| TCGAAGAATCGCGAGAAGGG+TGG | - | Chr8:51316878-51316897 | None:intergenic | 55.0% | |
| TGAATGGCCAAGCATCAGGG+TGG | - | Chr8:51317847-51317866 | None:intergenic | 55.0% | |
| TGCATGACCACCCTGATGCT+TGG | + | Chr8:51317837-51317856 | MsG0880044910.01.T01:CDS | 55.0% | |
| !! | ATCCCTGGTTTGGAAGAGGC+TGG | + | Chr8:51317701-51317720 | MsG0880044910.01.T01:CDS | 55.0% |
| !! | CTGGTTTGGAAGAGGCTGGA+TGG | + | Chr8:51317705-51317724 | MsG0880044910.01.T01:CDS | 55.0% |
| CGGTTCTGTCCCCGACTTTG+TGG | + | Chr8:51316941-51316960 | MsG0880044910.01.T01:CDS | 60.0% | |
| GAAGAAGTGGCGAAGGCCGA+AGG | - | Chr8:51316810-51316829 | None:intergenic | 60.0% | |
| GGGGACAGAACCGGAAGTAG+CGG | - | Chr8:51316934-51316953 | None:intergenic | 60.0% | |
| GTGACAACGCTCCTCCAGTC+TGG | + | Chr8:51318094-51318113 | MsG0880044910.01.T01:CDS | 60.0% | |
| TCGCGAGAAGGGTGGAATGG+AGG | - | Chr8:51316870-51316889 | None:intergenic | 60.0% | |
| ! | GGAATCCCAGACTGGCCCTT+CGG | + | Chr8:51316791-51316810 | MsG0880044910.01.T01:CDS | 60.0% |
| GAAGGCCGAAGGGCCAGTCT+GGG | - | Chr8:51316799-51316818 | None:intergenic | 65.0% | |
| CGAAGGCCGAAGGGCCAGTC+TGG | - | Chr8:51316800-51316819 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 51316789 | 51318132 | 51316789 | ID=MsG0880044910.01;Name=MsG0880044910.01 |
| Chr8 | mRNA | 51316789 | 51318132 | 51316789 | ID=MsG0880044910.01.T01;Parent=MsG0880044910.01;Name=MsG0880044910.01.T01;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|447 |
| Chr8 | exon | 51316789 | 51318132 | 51316789 | ID=MsG0880044910.01.T01:exon:21791;Parent=MsG0880044910.01.T01 |
| Chr8 | CDS | 51316789 | 51318132 | 51316789 | ID=MsG0880044910.01.T01:cds;Parent=MsG0880044910.01.T01 |
| Chr8 | mRNA | 51316897 | 51318132 | 51316897 | ID=MsG0880044910.01.T02;Parent=MsG0880044910.01;Name=MsG0880044910.01.T02;_AED=0.04;_eAED=0.04;_QI=0|-1|0|1|-1|0|1|0|411 |
| Chr8 | exon | 51316897 | 51318132 | 51316897 | ID=MsG0880044910.01.T02:exon:21792;Parent=MsG0880044910.01.T02 |
| Chr8 | CDS | 51316897 | 51318132 | 51316897 | ID=MsG0880044910.01.T02:cds;Parent=MsG0880044910.01.T02 |
| Chr8 | mRNA | 51317815 | 51318132 | 51317815 | ID=MsG0880044910.01.T03;Parent=MsG0880044910.01;Name=MsG0880044910.01.T03;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|0|1|0|105 |
| Chr8 | exon | 51317815 | 51318132 | 51317815 | ID=MsG0880044910.01.T03:exon:21793;Parent=MsG0880044910.01.T03 |
| Chr8 | CDS | 51317815 | 51318132 | 51317815 | ID=MsG0880044910.01.T03:cds;Parent=MsG0880044910.01.T03 |
| Chr8 | mRNA | 51317923 | 51318132 | 51317923 | ID=MsG0880044910.01.T04;Parent=MsG0880044910.01;Name=MsG0880044910.01.T04;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|0|1|0|69 |
| Chr8 | exon | 51317923 | 51318132 | 51317923 | ID=MsG0880044910.01.T04:exon:21794;Parent=MsG0880044910.01.T04 |
| Chr8 | CDS | 51317923 | 51318132 | 51317923 | ID=MsG0880044910.01.T04:cds;Parent=MsG0880044910.01.T04 |
| Gene Sequence |
| Protein sequence |
