AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047455.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047455.01.T01 MTR_4g127800 84.211 76 11 1 36 110 136 211 4.44e-32 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047455.01.T01 AT2G18950 81.667 60 11 0 51 110 134 193 6.68e-26 99.4
MsG0880047455.01.T01 AT2G18950 81.667 60 11 0 51 110 134 193 1.47e-25 99.4

Find 22 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGTATCTTCTTCAATTC+TGG 0.148269 8:-86612310 MsG0880047455.01.T01:CDS
CAATATAAATGTTCATAAAC+AGG 0.328480 8:+86612812 None:intergenic
TTTATGAACATTTATATTGT+TGG 0.348917 8:-86612808 MsG0880047455.01.T01:CDS
CCGGATGCCAAGGGAAGATA+TGG 0.362386 8:+86612363 None:intergenic
AATATTGACCATAAAATTTG+AGG 0.383786 8:+86613117 None:intergenic
ACAGGCATTAAGCATAATTT+CGG 0.406456 8:-86613020 MsG0880047455.01.T01:intron
TGAAGAAGATACAACAATAA+TGG 0.408142 8:+86612317 None:intergenic
CCATATCTTCCCTTGGCATC+CGG 0.448076 8:-86612363 MsG0880047455.01.T01:CDS
GGAGAATATTCCTTTGCAAC+TGG 0.454611 8:-86612342 MsG0880047455.01.T01:CDS
GGAATATTCTCCGGATGCCA+AGG 0.511719 8:+86612353 None:intergenic
TTCGGTGTCTCTCCTTGCAG+TGG 0.543367 8:-86613002 MsG0880047455.01.T01:CDS
TACCTTGTCTATTTCAACAT+CGG 0.553008 8:+86612771 None:intergenic
AAACAAACCATATCTTCCCT+TGG 0.565168 8:-86612370 MsG0880047455.01.T01:CDS
AATAATGGCACCAGTTGCAA+AGG 0.570603 8:+86612332 None:intergenic
AGTTGCAAAGGAATATTCTC+CGG 0.590284 8:+86612344 None:intergenic
ATTAGTTCAGAGTAAATCTG+AGG 0.594619 8:+86613140 None:intergenic
CTGATAATTTCTCCACTGCA+AGG 0.606965 8:+86612990 None:intergenic
TTAGTTCAGAGTAAATCTGA+GGG 0.621965 8:+86613141 None:intergenic
GAATATTCTCCGGATGCCAA+GGG 0.633819 8:+86612354 None:intergenic
AATATAAATGTTCATAAACA+GGG 0.649234 8:+86612813 None:intergenic
GTCCGATGTTGAAATAGACA+AGG 0.665037 8:-86612773 MsG0880047455.01.T01:intron
TAGTTCAGAGTAAATCTGAG+GGG 0.757706 8:+86613142 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAAATGTTCATAAACA+GGG + Chr8:86612611-86612630 None:intergenic 15.0%
!! TTTACATCTTACAAATTATT+TGG - Chr8:86612969-86612988 MsG0880047455.01.T01:CDS 15.0%
!! TTTATGAACATTTATATTGT+TGG - Chr8:86612613-86612632 MsG0880047455.01.T01:intron 15.0%
!!! AATAATTTGTAAGATGTAAA+AGG + Chr8:86612969-86612988 None:intergenic 15.0%
!! AAAAACAATTATGAAGTTAG+GGG + Chr8:86612553-86612572 None:intergenic 20.0%
!! AATATTGACCATAAAATTTG+AGG + Chr8:86612307-86612326 None:intergenic 20.0%
!! ACAAAAACAATTATGAAGTT+AGG + Chr8:86612555-86612574 None:intergenic 20.0%
!! ACTATTAGAATAAAACAATC+TGG - Chr8:86612681-86612700 MsG0880047455.01.T01:intron 20.0%
!! CAAAAACAATTATGAAGTTA+GGG + Chr8:86612554-86612573 None:intergenic 20.0%
!! CAATATAAATGTTCATAAAC+AGG + Chr8:86612612-86612631 None:intergenic 20.0%
!! GTAAAATATCAGTGAAAAAT+GGG + Chr8:86613153-86613172 None:intergenic 20.0%
!! TAAACTTGAAAGAGAAATAA+CGG + Chr8:86612505-86612524 None:intergenic 20.0%
!! TGTAAAATATCAGTGAAAAA+TGG + Chr8:86613154-86613173 None:intergenic 20.0%
!!! CTGCATCATATTTTTTATAT+TGG + Chr8:86612751-86612770 None:intergenic 20.0%
!!! GTTTGTTTTATTTATGTTTC+AGG - Chr8:86612573-86612592 MsG0880047455.01.T01:intron 20.0%
! AAAAATGGGATGAATCTATA+TGG + Chr8:86613139-86613158 None:intergenic 25.0%
! ACATCTTACAAATTATTTGG+AGG - Chr8:86612972-86612991 MsG0880047455.01.T01:CDS 25.0%
! ATCTGTATGAATCACTTATT+TGG - Chr8:86612338-86612357 MsG0880047455.01.T01:CDS 25.0%
! GCGCAAAATTAATTTATTAG+AGG + Chr8:86612708-86612727 None:intergenic 25.0%
! TCAATATATAAACCAGAGTT+TGG + Chr8:86612889-86612908 None:intergenic 25.0%
! TGAAGAAGATACAACAATAA+TGG + Chr8:86613107-86613126 None:intergenic 25.0%
!! ATAAATTAATTTTGCGCCTT+TGG - Chr8:86612711-86612730 MsG0880047455.01.T01:intron 25.0%
!! GAACTAATCCTCAAATTTTA+TGG - Chr8:86612296-86612315 MsG0880047455.01.T01:CDS 25.0%
!! TAAATTAATTTTGCGCCTTT+GGG - Chr8:86612712-86612731 MsG0880047455.01.T01:intron 25.0%
!! TGTTTCTAAATATCCAACTT+TGG + Chr8:86612795-86612814 None:intergenic 25.0%
!! TTGGATGATTGTCTTATTAT+GGG - Chr8:86612357-86612376 MsG0880047455.01.T01:CDS 25.0%
!! TTTGGATGATTGTCTTATTA+TGG - Chr8:86612356-86612375 MsG0880047455.01.T01:CDS 25.0%
!!! TATATCTCCACTATTTTTAC+AGG - Chr8:86612455-86612474 MsG0880047455.01.T01:intron 25.0%
ACAGGCATTAAGCATAATTT+CGG - Chr8:86612401-86612420 MsG0880047455.01.T01:intron 30.0%
ATTAGTTCAGAGTAAATCTG+AGG + Chr8:86612284-86612303 None:intergenic 30.0%
CAATACACCTGTAAAAATAG+TGG + Chr8:86612465-86612484 None:intergenic 30.0%
TACCTTGTCTATTTCAACAT+CGG + Chr8:86612653-86612672 None:intergenic 30.0%
TATTTGGAGGAACTATCATA+TGG - Chr8:86612985-86613004 MsG0880047455.01.T01:CDS 30.0%
TGTTGTATCTTCTTCAATTC+TGG - Chr8:86613111-86613130 MsG0880047455.01.T01:CDS 30.0%
TTAGTTCAGAGTAAATCTGA+GGG + Chr8:86612283-86612302 None:intergenic 30.0%
TTATTGTAGTCTCTGATATG+TGG - Chr8:86612927-86612946 MsG0880047455.01.T01:intron 30.0%
! TGGATGATTGTCTTATTATG+GGG - Chr8:86612358-86612377 MsG0880047455.01.T01:CDS 30.0%
!! AAAGAGAAATAACGGATTTG+GGG + Chr8:86612497-86612516 None:intergenic 30.0%
!! GAAAGAGAAATAACGGATTT+GGG + Chr8:86612498-86612517 None:intergenic 30.0%
!! TATTTTTACAGGTGTATTGG+AGG - Chr8:86612466-86612485 MsG0880047455.01.T01:intron 30.0%
!! TGAAAGAGAAATAACGGATT+TGG + Chr8:86612499-86612518 None:intergenic 30.0%
!!! CACTATTTTTACAGGTGTAT+TGG - Chr8:86612463-86612482 MsG0880047455.01.T01:intron 30.0%
AAACAAACCATATCTTCCCT+TGG - Chr8:86613051-86613070 MsG0880047455.01.T01:intron 35.0%
AGTTGCAAAGGAATATTCTC+CGG + Chr8:86613080-86613099 None:intergenic 35.0%
GCTCTTAGTTAATCCAAAGT+TGG - Chr8:86612779-86612798 MsG0880047455.01.T01:CDS 35.0%
TAAATATCCTATTGCTCCCT+TGG - Chr8:86612523-86612542 MsG0880047455.01.T01:intron 35.0%
TAGTTCAGAGTAAATCTGAG+GGG + Chr8:86612282-86612301 None:intergenic 35.0%
! GTAAAAGGATGTGTGTTTTC+TGG + Chr8:86612954-86612973 None:intergenic 35.0%
!! TGATTTTGACAACCAAACTC+TGG - Chr8:86612874-86612893 MsG0880047455.01.T01:intron 35.0%
AATAATGGCACCAGTTGCAA+AGG + Chr8:86613092-86613111 None:intergenic 40.0%
CTGATAATTTCTCCACTGCA+AGG + Chr8:86612434-86612453 None:intergenic 40.0%
GACTATATCTGTGTTTGGAC+AGG - Chr8:86612383-86612402 MsG0880047455.01.T01:intron 40.0%
GGAGAATATTCCTTTGCAAC+TGG - Chr8:86613079-86613098 MsG0880047455.01.T01:intron 40.0%
GTCCGATGTTGAAATAGACA+AGG - Chr8:86612648-86612667 MsG0880047455.01.T01:intron 40.0%
GAATATTCTCCGGATGCCAA+GGG + Chr8:86613070-86613089 None:intergenic 45.0%
GGAAAGTTTCAGAAGCCCAA+AGG + Chr8:86612730-86612749 None:intergenic 45.0%
GGGCTGACTATATCTGTGTT+TGG - Chr8:86612378-86612397 MsG0880047455.01.T01:intron 45.0%
ATGAAGTTAGGGGCAAGCCA+AGG + Chr8:86612543-86612562 None:intergenic 50.0%
CCATATCTTCCCTTGGCATC+CGG - Chr8:86613058-86613077 MsG0880047455.01.T01:intron 50.0%
GGAATATTCTCCGGATGCCA+AGG + Chr8:86613071-86613090 None:intergenic 50.0%
TGAAGTTAGGGGCAAGCCAA+GGG + Chr8:86612542-86612561 None:intergenic 50.0%
CCGGATGCCAAGGGAAGATA+TGG + Chr8:86613061-86613080 None:intergenic 55.0%
!! TTCGGTGTCTCTCCTTGCAG+TGG - Chr8:86612419-86612438 MsG0880047455.01.T01:intron 55.0%
GGGCAAGCCAAGGGAGCAAT+AGG + Chr8:86612533-86612552 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 86612254 86613189 86612254 ID=MsG0880047455.01;Name=MsG0880047455.01
Chr8 mRNA 86612254 86613189 86612254 ID=MsG0880047455.01.T01;Parent=MsG0880047455.01;Name=MsG0880047455.01.T01;_AED=0.47;_eAED=0.62;_QI=0|0.66|0.5|1|1|1|4|0|128
Chr8 exon 86613102 86613189 86613102 ID=MsG0880047455.01.T01:exon:2130;Parent=MsG0880047455.01.T01
Chr8 exon 86612956 86613038 86612956 ID=MsG0880047455.01.T01:exon:2129;Parent=MsG0880047455.01.T01
Chr8 exon 86612774 86612848 86612774 ID=MsG0880047455.01.T01:exon:2128;Parent=MsG0880047455.01.T01
Chr8 exon 86612254 86612394 86612254 ID=MsG0880047455.01.T01:exon:2127;Parent=MsG0880047455.01.T01
Chr8 CDS 86613102 86613189 86613102 ID=MsG0880047455.01.T01:cds;Parent=MsG0880047455.01.T01
Chr8 CDS 86612956 86613038 86612956 ID=MsG0880047455.01.T01:cds;Parent=MsG0880047455.01.T01
Chr8 CDS 86612774 86612848 86612774 ID=MsG0880047455.01.T01:cds;Parent=MsG0880047455.01.T01
Chr8 CDS 86612254 86612394 86612254 ID=MsG0880047455.01.T01:cds;Parent=MsG0880047455.01.T01
Gene Sequence

>MsG0880047455.01.T01

ATGGGCACAGTTAAGTTTCATTTTTCCCCTCAGATTTACTCTGAACTAATCCTCAAATTTTATGGTCAATATTTGATACAATTCATCTGCATTAAGCATAATTTCGGTGTCTCTCCTTGCAGTGGAGAAATTATCAGATATATCTCCACTATTTTTACAGGTGTATTGGAGGCTGTAGTAGCTGCCCTGTTTATGAACATTTATATTGTTGGTTTGAATCAATTGTCCGATGTTGAAATAGACAAGATAAACAAACCATATCTTCCCTTGGCATCCGGAGAATATTCCTTTGCAACTGGTGCCATTATTGTTGTATCTTCTTCAATTCTGGTGCCATATAGATTCATCCCATTTTTCACTGATATTTTACAAGATGAGCTATACTGA
Protein sequence

>MsG0880047455.01.T01

MGTVKFHFSPQIYSELILKFYGQYLIQFICIKHNFGVSPCSGEIIRYISTIFTGVLEAVVAALFMNIYIVGLNQLSDVEIDKINKPYLPLASGEYSFATGAIIVVSSSILVPYRFIPFFTDILQDELY*