AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047090.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047090.01.T01 MTR_5g069055 97.000 100 3 0 1 100 228 327 1.05e-63 197
MsG0880047090.01.T01 MTR_5g069050 95.000 100 5 0 1 100 254 353 2.97e-61 191
MsG0880047090.01.T01 MTR_1g061050 81.000 100 19 0 1 100 228 327 3.37e-55 175
MsG0880047090.01.T01 MTR_5g096670 72.000 100 28 0 1 100 228 327 2.27e-47 155
MsG0880047090.01.T01 MTR_4g057670 56.436 101 41 2 1 100 272 370 4.28e-32 116
MsG0880047090.01.T01 MTR_4g071860 56.436 101 41 2 1 100 272 370 4.42e-32 116
MsG0880047090.01.T01 MTR_4g071880 54.455 101 43 2 1 100 258 356 3.19e-30 111
MsG0880047090.01.T01 MTR_1g109200 53.465 101 44 2 1 100 263 361 3.37e-30 111
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047090.01.T01 AT3G52930 84.000 100 16 0 1 100 228 327 6.79e-57 179
MsG0880047090.01.T01 AT2G36460 82.000 100 18 0 1 100 137 236 2.17e-55 173
MsG0880047090.01.T01 AT2G36460 82.000 100 18 0 1 100 137 236 2.17e-55 173
MsG0880047090.01.T01 AT2G36460 82.000 100 18 0 1 100 228 327 1.65e-54 174
MsG0880047090.01.T01 AT5G03690 81.188 101 18 1 1 100 136 236 6.04e-54 169
MsG0880047090.01.T01 AT5G03690 81.188 101 18 1 1 100 136 236 6.04e-54 169
MsG0880047090.01.T01 AT5G03690 81.188 101 18 1 1 100 228 328 3.93e-53 170
MsG0880047090.01.T01 AT5G03690 81.188 101 18 1 1 100 262 362 1.01e-52 170
MsG0880047090.01.T01 AT4G26530 74.000 100 26 0 1 100 228 327 1.17e-48 158
MsG0880047090.01.T01 AT4G26530 74.000 100 26 0 1 100 228 327 1.17e-48 158
MsG0880047090.01.T01 AT4G26530 74.000 100 26 0 1 100 228 327 1.17e-48 158
MsG0880047090.01.T01 AT4G26520 66.000 100 34 0 1 100 225 324 1.44e-42 142
MsG0880047090.01.T01 AT4G26520 66.000 100 34 0 1 100 225 324 1.44e-42 142
MsG0880047090.01.T01 AT4G26520 66.000 100 34 0 1 100 228 327 1.81e-42 142
MsG0880047090.01.T01 AT4G26520 66.000 100 34 0 1 100 228 327 1.97e-42 142
MsG0880047090.01.T01 AT2G21330 56.436 101 41 2 1 100 271 369 4.91e-32 116
MsG0880047090.01.T01 AT2G01140 54.455 101 43 2 1 100 263 361 3.91e-30 110
MsG0880047090.01.T01 AT4G38970 53.465 101 44 2 1 100 270 368 3.92e-29 108
MsG0880047090.01.T01 AT2G21330 49.505 101 38 3 1 100 271 359 9.00e-24 94.0

Find 36 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGACCCTTTCCTTCTCTTT+TGG 0.209134 8:-81799427 MsG0880047090.01.T01:CDS
CTTACCCTTGACTTGGTTGA+TGG 0.263243 8:+81799456 None:intergenic
ACCTTTGGTGCATCAGATCC+AGG 0.397934 8:+81799604 None:intergenic
ACAACCTCGGGTGCAACCTT+TGG 0.418995 8:+81799589 None:intergenic
GGCTAATTCTGAGCTACTCT+TGG 0.441157 8:-81799315 MsG0880047090.01.T01:CDS
AGTACCCTCAAGGCATGGTC+TGG 0.442363 8:-81799388 MsG0880047090.01.T01:CDS
ATGGCTTCTTACCCTTGACT+TGG 0.461709 8:+81799449 None:intergenic
CTCAAGAAGCTTTGTTGACA+AGG 0.475863 8:-81799342 MsG0880047090.01.T01:CDS
CCAGACAAGAAAACAACAGC+TGG 0.481066 8:+81799517 None:intergenic
GTGTGCTCAGCAACAACCTC+GGG 0.487306 8:+81799577 None:intergenic
AGCTGGGACTGCAGCAGGTA+CGG 0.503571 8:+81799534 None:intergenic
AGCTACTCTTGGAACTTACA+AGG 0.508128 8:-81799304 MsG0880047090.01.T01:CDS
CCCTTCCAAAAGAGAAGGAA+AGG 0.518782 8:+81799422 None:intergenic
ACAACAGCTGGGACTGCAGC+AGG 0.520425 8:+81799529 None:intergenic
CCCTGGATCTGATGCACCAA+AGG 0.525088 8:-81799605 MsG0880047090.01.T01:CDS
TGGTGGACAGAGTGAGGAAG+AGG 0.529702 8:-81799497 MsG0880047090.01.T01:CDS
TGACTTGGTTGATGGCATTG+AGG 0.532962 8:+81799464 None:intergenic
AAGCTTTGTTGACAAGGCTA+AGG 0.540872 8:-81799336 MsG0880047090.01.T01:CDS
AAGCCTAACATGGTTACCCC+TGG 0.543234 8:-81799622 None:intergenic
AAAGAGAAGGAAAGGGTCCA+TGG 0.554272 8:+81799430 None:intergenic
AAGTCAAGGGTAAGAAGCCA+TGG 0.558340 8:-81799447 MsG0880047090.01.T01:CDS
CTTGTCTGGTGGACAGAGTG+AGG 0.562758 8:-81799503 MsG0880047090.01.T01:CDS
GATGGCATTGAGGTTGACAC+TGG 0.575533 8:+81799474 None:intergenic
CCTTTGGTGCATCAGATCCA+GGG 0.582593 8:+81799605 None:intergenic
CAATGCCATCAACCAAGTCA+AGG 0.584504 8:-81799461 MsG0880047090.01.T01:CDS
TGCACCAAAGGTTGCACCCG+AGG 0.589398 8:-81799593 MsG0880047090.01.T01:CDS
AAGTGCCCTTCCAAAAGAGA+AGG 0.603752 8:+81799417 None:intergenic
AACAGAGTACCCTCAAGGCA+TGG 0.603839 8:-81799393 MsG0880047090.01.T01:CDS
AATGCCATCAACCAAGTCAA+GGG 0.616498 8:-81799460 MsG0880047090.01.T01:CDS
CCTTCCAAAAGAGAAGGAAA+GGG 0.621042 8:+81799423 None:intergenic
AGTGTGCTCAGCAACAACCT+CGG 0.636805 8:+81799576 None:intergenic
CAGACAAGAAAACAACAGCT+GGG 0.641298 8:+81799518 None:intergenic
CTTTGGTGCATCAGATCCAG+GGG 0.655083 8:+81799606 None:intergenic
TGGAAAAGAAGAAAATGTGA+AGG 0.663229 8:-81799368 MsG0880047090.01.T01:CDS
GCTACTCTTGGAACTTACAA+GGG 0.663928 8:-81799303 MsG0880047090.01.T01:CDS
ACTTCAACAGAGTACCCTCA+AGG 0.678437 8:-81799398 MsG0880047090.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TGGAAAAGAAGAAAATGTGA+AGG - Chr8:81799545-81799564 MsG0880047090.01.T01:CDS 30.0%
AATGCCATCAACCAAGTCAA+GGG - Chr8:81799453-81799472 MsG0880047090.01.T01:CDS 40.0%
AGCTACTCTTGGAACTTACA+AGG - Chr8:81799609-81799628 MsG0880047090.01.T01:CDS 40.0%
CAGACAAGAAAACAACAGCT+GGG + Chr8:81799398-81799417 None:intergenic 40.0%
CCTTCCAAAAGAGAAGGAAA+GGG + Chr8:81799493-81799512 None:intergenic 40.0%
GCTACTCTTGGAACTTACAA+GGG - Chr8:81799610-81799629 MsG0880047090.01.T01:CDS 40.0%
!! AAGCTTTGTTGACAAGGCTA+AGG - Chr8:81799577-81799596 MsG0880047090.01.T01:CDS 40.0%
!! CCTTTCCTTCTCTTTTGGAA+GGG - Chr8:81799491-81799510 MsG0880047090.01.T01:CDS 40.0%
!! CTCAAGAAGCTTTGTTGACA+AGG - Chr8:81799571-81799590 MsG0880047090.01.T01:CDS 40.0%
AAAGAGAAGGAAAGGGTCCA+TGG + Chr8:81799486-81799505 None:intergenic 45.0%
AAGTCAAGGGTAAGAAGCCA+TGG - Chr8:81799466-81799485 MsG0880047090.01.T01:CDS 45.0%
AAGTGCCCTTCCAAAAGAGA+AGG + Chr8:81799499-81799518 None:intergenic 45.0%
ATGGCTTCTTACCCTTGACT+TGG + Chr8:81799467-81799486 None:intergenic 45.0%
CAATGCCATCAACCAAGTCA+AGG - Chr8:81799452-81799471 MsG0880047090.01.T01:CDS 45.0%
CCAGACAAGAAAACAACAGC+TGG + Chr8:81799399-81799418 None:intergenic 45.0%
CCCTTCCAAAAGAGAAGGAA+AGG + Chr8:81799494-81799513 None:intergenic 45.0%
GGCTAATTCTGAGCTACTCT+TGG - Chr8:81799598-81799617 MsG0880047090.01.T01:CDS 45.0%
! ACTTCAACAGAGTACCCTCA+AGG - Chr8:81799515-81799534 MsG0880047090.01.T01:CDS 45.0%
! CCCTTTCCTTCTCTTTTGGA+AGG - Chr8:81799490-81799509 MsG0880047090.01.T01:CDS 45.0%
! TGGACCCTTTCCTTCTCTTT+TGG - Chr8:81799486-81799505 MsG0880047090.01.T01:CDS 45.0%
! TTTTCCAGACCATGCCTTGA+GGG + Chr8:81799532-81799551 None:intergenic 45.0%
!! CTTACCCTTGACTTGGTTGA+TGG + Chr8:81799460-81799479 None:intergenic 45.0%
!! TGACTTGGTTGATGGCATTG+AGG + Chr8:81799452-81799471 None:intergenic 45.0%
!!! CCAGCTGTTGTTTTCTTGTC+TGG - Chr8:81799396-81799415 MsG0880047090.01.T01:CDS 45.0%
!!! GCTGTTGTTTTCTTGTCTGG+TGG - Chr8:81799399-81799418 MsG0880047090.01.T01:CDS 45.0%
AGTGTGCTCAGCAACAACCT+CGG + Chr8:81799340-81799359 None:intergenic 50.0%
! AACAGAGTACCCTCAAGGCA+TGG - Chr8:81799520-81799539 MsG0880047090.01.T01:CDS 50.0%
! ACCTTTGGTGCATCAGATCC+AGG + Chr8:81799312-81799331 None:intergenic 50.0%
! CCTTTGGTGCATCAGATCCA+GGG + Chr8:81799311-81799330 None:intergenic 50.0%
! CTTTGGTGCATCAGATCCAG+GGG + Chr8:81799310-81799329 None:intergenic 50.0%
! CTTTTCCAGACCATGCCTTG+AGG + Chr8:81799533-81799552 None:intergenic 50.0%
! GATGGCATTGAGGTTGACAC+TGG + Chr8:81799442-81799461 None:intergenic 50.0%
ACAACCTCGGGTGCAACCTT+TGG + Chr8:81799327-81799346 None:intergenic 55.0%
AGTACCCTCAAGGCATGGTC+TGG - Chr8:81799525-81799544 MsG0880047090.01.T01:CDS 55.0%
CCCTGGATCTGATGCACCAA+AGG - Chr8:81799308-81799327 MsG0880047090.01.T01:CDS 55.0%
GTGTGCTCAGCAACAACCTC+GGG + Chr8:81799339-81799358 None:intergenic 55.0%
! CTTGTCTGGTGGACAGAGTG+AGG - Chr8:81799410-81799429 MsG0880047090.01.T01:CDS 55.0%
! TGGTGGACAGAGTGAGGAAG+AGG - Chr8:81799416-81799435 MsG0880047090.01.T01:CDS 55.0%
ACAACAGCTGGGACTGCAGC+AGG + Chr8:81799387-81799406 None:intergenic 60.0%
AGCTGGGACTGCAGCAGGTA+CGG + Chr8:81799382-81799401 None:intergenic 60.0%
TGCACCAAAGGTTGCACCCG+AGG - Chr8:81799320-81799339 MsG0880047090.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 81799300 81799635 81799300 ID=MsG0880047090.01;Name=MsG0880047090.01
Chr8 mRNA 81799300 81799635 81799300 ID=MsG0880047090.01.T01;Parent=MsG0880047090.01;Name=MsG0880047090.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|111
Chr8 exon 81799300 81799635 81799300 ID=MsG0880047090.01.T01:exon:35202;Parent=MsG0880047090.01.T01
Chr8 CDS 81799300 81799635 81799300 ID=MsG0880047090.01.T01:cds;Parent=MsG0880047090.01.T01
Gene Sequence

>MsG0880047090.01.T01

ATGGTTACCCCTGGATCTGATGCACCAAAGGTTGCACCCGAGGTTGTTGCTGAGCACACTGTTAGAGCTTTGCAGAGAACCGTACCTGCTGCAGTCCCAGCTGTTGTTTTCTTGTCTGGTGGACAGAGTGAGGAAGAGGCCAGTGTCAACCTCAATGCCATCAACCAAGTCAAGGGTAAGAAGCCATGGACCCTTTCCTTCTCTTTTGGAAGGGCACTTCAACAGAGTACCCTCAAGGCATGGTCTGGAAAAGAAGAAAATGTGAAGGCTGCTCAAGAAGCTTTGTTGACAAGGCTAAGGCTAATTCTGAGCTACTCTTGGAACTTACAAGGGTAA

Protein sequence

>MsG0880047090.01.T01

MVTPGSDAPKVAPEVVAEHTVRALQRTVPAAVPAVVFLSGGQSEEEASVNLNAINQVKGKKPWTLSFSFGRALQQSTLKAWSGKEENVKAAQEALLTRLRLILSYSWNLQG*