AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045371.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045371.01.T01 MTR_8g070510 80.102 196 12 2 1 169 1 196 2.03e-107 308
MsG0880045371.01.T01 MTR_8g070510 79.688 192 12 2 5 169 1 192 4.98e-104 299
MsG0880045371.01.T01 MTR_7g451050 31.250 176 92 5 18 166 18 191 8.55e-22 88.6
MsG0880045371.01.T01 MTR_7g451050 31.250 176 92 5 18 166 36 209 1.34e-21 88.6
MsG0880045371.01.T01 MTR_7g451050 30.726 179 95 5 18 169 18 194 1.48e-21 88.6
MsG0880045371.01.T01 MTR_7g451050 30.726 179 95 5 18 169 36 212 1.65e-21 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045371.01.T01 AT4G26470 58.549 193 51 3 1 166 1 191 2.21e-72 218
MsG0880045371.01.T01 AT4G26470 57.143 196 55 3 1 169 1 194 2.77e-71 216
MsG0880045371.01.T01 AT4G26470 57.143 196 55 3 1 169 64 257 3.88e-70 215
MsG0880045371.01.T01 AT3G24110 31.937 191 98 5 9 169 12 200 5.79e-20 84.7
MsG0880045371.01.T01 AT3G24110 31.937 191 98 5 9 169 9 197 5.83e-20 84.3

Find 42 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTATTCTTAGACAAGAATA+AGG 0.312157 8:+58299537 MsG0880045371.01.T01:CDS
TCAAATGTGGCCTCCAGATT+TGG 0.325162 8:-58299496 None:intergenic
AACCAAGCTTGAGGCTAAAA+TGG 0.325884 8:+58297854 MsG0880045371.01.T01:CDS
ACATCCGGGGAACGTTCTTC+TGG 0.336685 8:+58299607 MsG0880045371.01.T01:CDS
ACGAATTGGCATGCCAAATC+TGG 0.337140 8:+58299483 MsG0880045371.01.T01:CDS
GGTAGGTTTCTACTTCAAAT+TGG 0.337511 8:+58297999 MsG0880045371.01.T01:CDS
TATAGTGTTGAATGATTTCA+TGG 0.344014 8:-58297913 None:intergenic
AGAATAGCCATGAAAAGATT+TGG 0.372821 8:+58299631 MsG0880045371.01.T01:CDS
AACACACACATGCTCATCTT+AGG 0.417331 8:+58299718 MsG0880045371.01.T01:CDS
AGGGCCATCTTTGAGCAGTT+TGG 0.430873 8:+58297978 MsG0880045371.01.T01:CDS
ATTGTCTAGAAATCACGAAT+TGG 0.450354 8:+58299469 MsG0880045371.01.T01:intron
ATGTAGTTTCATTTATAGCT+TGG 0.465153 8:-58299588 None:intergenic
GCTATAAATGAAACTACATC+CGG 0.467940 8:+58299592 MsG0880045371.01.T01:CDS
ATGGGAGGTGCAATGGGAAA+GGG 0.479453 8:+58297801 MsG0880045371.01.T01:CDS
GATACTGCATGAAGTGCTGC+AGG 0.484247 8:-58298837 None:intergenic
GATGGGAGGTGCAATGGGAA+AGG 0.497322 8:+58297800 None:intergenic
GGAGGCCACATTTGAGACAT+TGG 0.509823 8:+58299504 MsG0880045371.01.T01:CDS
TTGGCGATGGGAGGTGCAAT+GGG 0.518653 8:+58297795 None:intergenic
ATTGGCGATGGGAGGTGCAA+TGG 0.524730 8:+58297794 None:intergenic
CCATCTTTGAGCAGTTTGGT+AGG 0.526543 8:+58297982 MsG0880045371.01.T01:CDS
TGTGTTCAAGCAAGCTTGAA+TGG 0.527679 8:-58299701 None:intergenic
ATGAGAGCTTCAGAAAATGC+AGG 0.530176 8:+58297958 MsG0880045371.01.T01:CDS
CCTACCAAACTGCTCAAAGA+TGG 0.539084 8:-58297982 None:intergenic
TTCTTAGACAAGAATAAGGA+CGG 0.561005 8:+58299541 MsG0880045371.01.T01:CDS
TCTTCCAGAAGAACGTTCCC+CGG 0.576812 8:-58299611 None:intergenic
CTATAAATGAAACTACATCC+GGG 0.577846 8:+58299593 MsG0880045371.01.T01:CDS
CTGCAGGGTCATCTTTGAGA+AGG 0.604557 8:-58298821 None:intergenic
TGGTTGAAGCAATGCAACGG+AGG 0.619505 8:+58297874 MsG0880045371.01.T01:CDS
TCTTAGACAAGAATAAGGAC+GGG 0.620398 8:+58299542 MsG0880045371.01.T01:CDS
GTTGATTGATTGGCGATGGG+AGG 0.623063 8:+58297786 None:intergenic
GGTTGAAGCAATGCAACGGA+GGG 0.624758 8:+58297875 MsG0880045371.01.T01:CDS
ATCAACCAATGTCTCAAATG+TGG 0.635646 8:-58299509 None:intergenic
GGCACTTGAGTGCATATGTG+TGG 0.638225 8:-58299680 None:intergenic
ATATGTGTGGAATTTAAACA+AGG 0.640636 8:-58299667 None:intergenic
TGAGAGCTTCAGAAAATGCA+GGG 0.640702 8:+58297959 MsG0880045371.01.T01:CDS
AATTGGCATGCCAAATCTGG+AGG 0.644569 8:+58299486 MsG0880045371.01.T01:CDS
AAATGGTTGAAGCAATGCAA+CGG 0.650809 8:+58297871 MsG0880045371.01.T01:CDS
GGTAGCAGAAACCAAGCTTG+AGG 0.658820 8:+58297845 MsG0880045371.01.T01:CDS
TATAAATGAAACTACATCCG+GGG 0.670677 8:+58299594 MsG0880045371.01.T01:CDS
ATGCAACGGAGGGAATGTCA+AGG 0.680566 8:+58297885 MsG0880045371.01.T01:CDS
TATGTGTGGAATTTAAACAA+GGG 0.682963 8:-58299666 None:intergenic
ATACTGCATGAAGTGCTGCA+GGG 0.692431 8:-58298836 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTAAAATTTCAAAATTAT+AGG - Chr8:58299314-58299333 None:intergenic 10.0%
!! CTGATATAAATATAGTTTAT+TGG + Chr8:58298054-58298073 MsG0880045371.01.T01:intron 15.0%
!! GAAAATTACATATTTAATTC+AGG + Chr8:58299195-58299214 MsG0880045371.01.T01:intron 15.0%
!! GTATATATGTTTATTGTAAT+TGG + Chr8:58298596-58298615 MsG0880045371.01.T01:intron 15.0%
!! TTACAATCACATAAAAAAAA+TGG - Chr8:58298486-58298505 None:intergenic 15.0%
!!! GAATATTATTTGAGTTTAAT+GGG + Chr8:58298960-58298979 MsG0880045371.01.T01:intron 15.0%
!!! TAACTGCATTTTTAAATTAA+TGG - Chr8:58299055-58299074 None:intergenic 15.0%
!!! TAAGCTATTTTTTTAACAAA+AGG + Chr8:58298207-58298226 MsG0880045371.01.T01:intron 15.0%
!!! TTTTCTAAATAAAATGCAAA+AGG - Chr8:58299180-58299199 None:intergenic 15.0%
!! AAAAATGCAGTTATAAAAGT+GGG + Chr8:58299062-58299081 MsG0880045371.01.T01:intron 20.0%
!! CAAAAATAGTATGGATTTAA+AGG - Chr8:58299156-58299175 None:intergenic 20.0%
!! TAAAAATGCAGTTATAAAAG+TGG + Chr8:58299061-58299080 MsG0880045371.01.T01:intron 20.0%
!! TAAACATATATACGACAATT+TGG - Chr8:58298589-58298608 None:intergenic 20.0%
!! TATAAATTATCAACGAAAAC+AGG - Chr8:58299414-58299433 None:intergenic 20.0%
!!! ATGTGATTTTAATGATGATT+TGG + Chr8:58298755-58298774 MsG0880045371.01.T01:CDS 20.0%
!!! GGAATATTATTTGAGTTTAA+TGG + Chr8:58298959-58298978 MsG0880045371.01.T01:intron 20.0%
!!! TGTGATTTTAATGATGATTT+GGG + Chr8:58298756-58298775 MsG0880045371.01.T01:CDS 20.0%
! AATGGTATGACATGAAAAAA+TGG - Chr8:58299037-58299056 None:intergenic 25.0%
! AATTTGAAAGGATAGAGAAA+TGG - Chr8:58299439-58299458 None:intergenic 25.0%
! ATATGTGTGGAATTTAAACA+AGG - Chr8:58299670-58299689 None:intergenic 25.0%
! ATCATAGAGAACAATTTGAA+AGG - Chr8:58299451-58299470 None:intergenic 25.0%
! ATGTAGTTTCATTTATAGCT+TGG - Chr8:58299591-58299610 None:intergenic 25.0%
! GTTGATGTTTGAATTATTTC+GGG + Chr8:58298910-58298929 MsG0880045371.01.T01:intron 25.0%
! TATGTGTGGAATTTAAACAA+GGG - Chr8:58299669-58299688 None:intergenic 25.0%
! TATTTAATTCAGGAGATGTT+TGG + Chr8:58299205-58299224 MsG0880045371.01.T01:intron 25.0%
! TGATCCTATTGTATAAATTC+AGG - Chr8:58298080-58298099 None:intergenic 25.0%
! TGTATTCTTAGACAAGAATA+AGG + Chr8:58299537-58299556 MsG0880045371.01.T01:CDS 25.0%
! TTTATTTCCATAAACTCTCT+AGG - Chr8:58298285-58298304 None:intergenic 25.0%
!! ATACATTTAACTTCAGAGAA+TGG + Chr8:58298938-58298957 MsG0880045371.01.T01:intron 25.0%
!! GGTTGATGTTTGAATTATTT+CGG + Chr8:58298909-58298928 MsG0880045371.01.T01:intron 25.0%
!! GTTAAGAAGTTTTTCTGTTA+TGG + Chr8:58299253-58299272 MsG0880045371.01.T01:intron 25.0%
!! TATAGTGTTGAATGATTTCA+TGG - Chr8:58297916-58297935 None:intergenic 25.0%
!! TTAAGTTGTTTACTCAAACT+CGG - Chr8:58298137-58298156 None:intergenic 25.0%
!!! CATCAATTTTTGGGAATTTT+AGG - Chr8:58297941-58297960 None:intergenic 25.0%
!!! TGCTTTTGAAAACAACTTAT+AGG - Chr8:58298862-58298881 None:intergenic 25.0%
AGAATAGCCATGAAAAGATT+TGG + Chr8:58299631-58299650 MsG0880045371.01.T01:CDS 30.0%
ATAATTCAAACATCAACCAG+AGG - Chr8:58298907-58298926 None:intergenic 30.0%
ATATAAGCACTTACATCACA+TGG - Chr8:58298186-58298205 None:intergenic 30.0%
ATTGTCTAGAAATCACGAAT+TGG + Chr8:58299469-58299488 MsG0880045371.01.T01:intron 30.0%
CAATTATCAAGCTCATTCAA+AGG + Chr8:58298509-58298528 MsG0880045371.01.T01:intron 30.0%
CTATAAATGAAACTACATCC+GGG + Chr8:58299593-58299612 MsG0880045371.01.T01:CDS 30.0%
GCAAAAGGACAAAAATAGTA+TGG - Chr8:58299165-58299184 None:intergenic 30.0%
GCTATAAATGAAACTACATC+CGG + Chr8:58299592-58299611 MsG0880045371.01.T01:CDS 30.0%
GTATGTCAGCAAAAATGAAA+TGG + Chr8:58299564-58299583 MsG0880045371.01.T01:CDS 30.0%
TATAAATGAAACTACATCCG+GGG + Chr8:58299594-58299613 MsG0880045371.01.T01:CDS 30.0%
TTATAAAAGTGGGATGATGT+GGG + Chr8:58299072-58299091 MsG0880045371.01.T01:intron 30.0%
TTCTTAGACAAGAATAAGGA+CGG + Chr8:58299541-58299560 MsG0880045371.01.T01:CDS 30.0%
TTGGCCTGAATTTATACAAT+AGG + Chr8:58298073-58298092 MsG0880045371.01.T01:intron 30.0%
! ATAAACTGAAAACCAGCTTT+TGG + Chr8:58298300-58298319 MsG0880045371.01.T01:intron 30.0%
! CTAATTTACACCGACATTTT+AGG - Chr8:58298986-58299005 None:intergenic 30.0%
! TGAAGCTCTCATCAATTTTT+GGG - Chr8:58297950-58297969 None:intergenic 30.0%
! TGAATCACCCAAAAAGTTTT+GGG + Chr8:58297824-58297843 MsG0880045371.01.T01:CDS 30.0%
!!! CTGGAAATTTCTTTTTTCTG+AGG + Chr8:58298707-58298726 MsG0880045371.01.T01:intron 30.0%
AAAAAATGGAGGACTCTCAT+TGG - Chr8:58299023-58299042 None:intergenic 35.0%
AAATGGTTGAAGCAATGCAA+CGG + Chr8:58297871-58297890 MsG0880045371.01.T01:CDS 35.0%
AAGCTATCCTAGAGAGTTTA+TGG + Chr8:58298275-58298294 MsG0880045371.01.T01:intron 35.0%
ACTGACATAAGCACTTGTTT+GGG - Chr8:58298367-58298386 None:intergenic 35.0%
ATCAACCAATGTCTCAAATG+TGG - Chr8:58299512-58299531 None:intergenic 35.0%
CATTAACCTATGTTTGTCAC+CGG + Chr8:58298552-58298571 MsG0880045371.01.T01:intron 35.0%
GGTAGGTTTCTACTTCAAAT+TGG + Chr8:58297999-58298018 MsG0880045371.01.T01:CDS 35.0%
GGTATGACATGAAAAAATGG+AGG - Chr8:58299034-58299053 None:intergenic 35.0%
GTTATAAAAGTGGGATGATG+TGG + Chr8:58299071-58299090 MsG0880045371.01.T01:intron 35.0%
TACTGACATAAGCACTTGTT+TGG - Chr8:58298368-58298387 None:intergenic 35.0%
TCTTAGACAAGAATAAGGAC+GGG + Chr8:58299542-58299561 MsG0880045371.01.T01:CDS 35.0%
! ACATTGATTGAGAGAGCTTA+TGG - Chr8:58298344-58298363 None:intergenic 35.0%
! CAACTGACCAAATCTTTTCA+TGG - Chr8:58299641-58299660 None:intergenic 35.0%
! CTGAAGCTCTCATCAATTTT+TGG - Chr8:58297951-58297970 None:intergenic 35.0%
! GTGAATCACCCAAAAAGTTT+TGG + Chr8:58297823-58297842 MsG0880045371.01.T01:CDS 35.0%
! GTTTAATGGGCCTAAAATGT+CGG + Chr8:58298973-58298992 MsG0880045371.01.T01:intron 35.0%
! TCTGCTACCCAAAACTTTTT+GGG - Chr8:58297834-58297853 None:intergenic 35.0%
! TTCTGCTACCCAAAACTTTT+TGG - Chr8:58297835-58297854 None:intergenic 35.0%
!! GCTACTTCTTGCTTTTACTT+TGG - Chr8:58298105-58298124 None:intergenic 35.0%
AACACACACATGCTCATCTT+AGG + Chr8:58299718-58299737 MsG0880045371.01.T01:CDS 40.0%
AACCAAGCTTGAGGCTAAAA+TGG + Chr8:58297854-58297873 MsG0880045371.01.T01:CDS 40.0%
AGCAATGCAATGTAGTAGGA+AGG + Chr8:58299365-58299384 MsG0880045371.01.T01:intron 40.0%
ATGAGAGCTTCAGAAAATGC+AGG + Chr8:58297958-58297977 MsG0880045371.01.T01:CDS 40.0%
CCCATTAGAATCTTCATCTG+CGG - Chr8:58298648-58298667 None:intergenic 40.0%
CCGCAGATGAAGATTCTAAT+GGG + Chr8:58298645-58298664 MsG0880045371.01.T01:intron 40.0%
CGCAGATGAAGATTCTAATG+GGG + Chr8:58298646-58298665 MsG0880045371.01.T01:intron 40.0%
CTGAAGCAATGCAATGTAGT+AGG + Chr8:58299361-58299380 MsG0880045371.01.T01:intron 40.0%
TCATTCAAAGGCTTCAACAG+TGG + Chr8:58298521-58298540 MsG0880045371.01.T01:intron 40.0%
TGAGAGCTTCAGAAAATGCA+GGG + Chr8:58297959-58297978 MsG0880045371.01.T01:CDS 40.0%
TGTGTTCAAGCAAGCTTGAA+TGG - Chr8:58299704-58299723 None:intergenic 40.0%
!! AACCATTTTAGCCTCAAGCT+TGG - Chr8:58297859-58297878 None:intergenic 40.0%
!! GAAGAAGTGTTTCAGTAAGC+TGG + Chr8:58298688-58298707 MsG0880045371.01.T01:intron 40.0%
AATTGGCATGCCAAATCTGG+AGG + Chr8:58299486-58299505 MsG0880045371.01.T01:CDS 45.0%
ACGAATTGGCATGCCAAATC+TGG + Chr8:58299483-58299502 MsG0880045371.01.T01:CDS 45.0%
CATGAAACACTGCGGCTATT+CGG + Chr8:58298425-58298444 MsG0880045371.01.T01:intron 45.0%
CCATCTTTGAGCAGTTTGGT+AGG + Chr8:58297982-58298001 MsG0880045371.01.T01:CDS 45.0%
CCTACCAAACTGCTCAAAGA+TGG - Chr8:58297985-58298004 None:intergenic 45.0%
CTTACGACATGTCCAAAAGC+TGG - Chr8:58298315-58298334 None:intergenic 45.0%
GCCGCAGATGAAGATTCTAA+TGG + Chr8:58298644-58298663 MsG0880045371.01.T01:intron 45.0%
GCCTAGATCATGAAACACTG+CGG + Chr8:58298417-58298436 MsG0880045371.01.T01:intron 45.0%
GGGATGATGTGGGAAAATAG+TGG + Chr8:58299082-58299101 MsG0880045371.01.T01:intron 45.0%
TCAAATGTGGCCTCCAGATT+TGG - Chr8:58299499-58299518 None:intergenic 45.0%
!! ATACTGCATGAAGTGCTGCA+GGG - Chr8:58298839-58298858 None:intergenic 45.0%
AGGGCCATCTTTGAGCAGTT+TGG + Chr8:58297978-58297997 MsG0880045371.01.T01:CDS 50.0%
ATGCAACGGAGGGAATGTCA+AGG + Chr8:58297885-58297904 MsG0880045371.01.T01:CDS 50.0%
CAATTTGGCCATGCATGTGC+CGG - Chr8:58298574-58298593 None:intergenic 50.0%
GCCGCAGTGTTTCATGATCT+AGG - Chr8:58298421-58298440 None:intergenic 50.0%
GGCACTTGAGTGCATATGTG+TGG - Chr8:58299683-58299702 None:intergenic 50.0%
GGTAGCAGAAACCAAGCTTG+AGG + Chr8:58297845-58297864 MsG0880045371.01.T01:CDS 50.0%
GGTTGAAGCAATGCAACGGA+GGG + Chr8:58297875-58297894 MsG0880045371.01.T01:CDS 50.0%
GTCTTGTGCATTCTGACCTC+TGG + Chr8:58298888-58298907 MsG0880045371.01.T01:intron 50.0%
TCTTCCAGAAGAACGTTCCC+CGG - Chr8:58299614-58299633 None:intergenic 50.0%
TGGTTGAAGCAATGCAACGG+AGG + Chr8:58297874-58297893 MsG0880045371.01.T01:CDS 50.0%
! ATGGGAGGTGCAATGGGAAA+GGG + Chr8:58297801-58297820 MsG0880045371.01.T01:CDS 50.0%
! CATGTGCCGGTGACAAACAT+AGG - Chr8:58298561-58298580 None:intergenic 50.0%
! CTGCAGGGTCATCTTTGAGA+AGG - Chr8:58298824-58298843 None:intergenic 50.0%
! GGAGGCCACATTTGAGACAT+TGG + Chr8:58299504-58299523 MsG0880045371.01.T01:CDS 50.0%
!! GATACTGCATGAAGTGCTGC+AGG - Chr8:58298840-58298859 None:intergenic 50.0%
ACATCCGGGGAACGTTCTTC+TGG + Chr8:58299607-58299626 MsG0880045371.01.T01:CDS 55.0%
GTTTGTCACCGGCACATGCA+TGG + Chr8:58298563-58298582 MsG0880045371.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 58297801 58299762 58297801 ID=MsG0880045371.01;Name=MsG0880045371.01
Chr8 mRNA 58297801 58299762 58297801 ID=MsG0880045371.01.T01;Parent=MsG0880045371.01;Name=MsG0880045371.01.T01;_AED=0.50;_eAED=0.60;_QI=0|0|0|1|0|0|3|0|201
Chr8 exon 58297801 58298020 58297801 ID=MsG0880045371.01.T01:exon:1662;Parent=MsG0880045371.01.T01
Chr8 exon 58298754 58298854 58298754 ID=MsG0880045371.01.T01:exon:1663;Parent=MsG0880045371.01.T01
Chr8 exon 58299478 58299762 58299478 ID=MsG0880045371.01.T01:exon:1664;Parent=MsG0880045371.01.T01
Chr8 CDS 58297801 58298020 58297801 ID=MsG0880045371.01.T01:cds;Parent=MsG0880045371.01.T01
Chr8 CDS 58298754 58298854 58298754 ID=MsG0880045371.01.T01:cds;Parent=MsG0880045371.01.T01
Chr8 CDS 58299478 58299762 58299478 ID=MsG0880045371.01.T01:cds;Parent=MsG0880045371.01.T01
Gene Sequence

>MsG0880045371.01.T01

ATGGGAGGTGCAATGGGAAAGGGTGAATCACCCAAAAAGTTTTGGGTAGCAGAAACCAAGCTTGAGGCTAAAATGGTTGAAGCAATGCAACGGAGGGAATGTCAAGGATGTTCCATGAAATCATTCAACACTATAATCCTAAAATTCCCAAAAATTGATGAGAGCTTCAGAAAATGCAGGGCCATCTTTGAGCAGTTTGGTAGGTTTCTACTTCAAATTGCATGTGATTTTAATGATGATTTGGGAATGAAGTTCAATGAGTTCATTGTACTTCTTTGTCTTGTCTACCTTCTCAAAGATGACCCTGCAGCACTTCATGCAAAATCACGAATTGGCATGCCAAATCTGGAGGCCACATTTGAGACATTGGTTGATACATTTGTATTCTTAGACAAGAATAAGGACGGGTATGTCAGCAAAAATGAAATGGTCCAAGCTATAAATGAAACTACATCCGGGGAACGTTCTTCTGGAAGAATAGCCATGAAAAGATTTGGTCAGTTGTCTCTCCCTTGTTTAAATTCCACACATATGCACTCAAGTGCCATTCAAGCTTGCTTGAACACACACATGCTCATCTTAGGAGTGTGCATTATCATATACTAA

Protein sequence

>MsG0880045371.01.T01

MGGAMGKGESPKKFWVAETKLEAKMVEAMQRRECQGCSMKSFNTIILKFPKIDESFRKCRAIFEQFGRFLLQIACDFNDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKNEMVQAINETTSGERSSGRIAMKRFGQLSLPCLNSTHMHSSAIQACLNTHMLILGVCIIIY*