AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0005490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0005490 MtrunA17_Chr1g0202341 74.852 676 168 1 4 679 12 685 0.0 1075
Msa0005490 MtrunA17_Chr5g0448761 43.701 643 342 6 36 666 53 687 0.0 560
Msa0005490 MtrunA17_Chr5g0440451 35.148 643 379 9 42 666 42 664 1.61e-132 416
Msa0005490 MtrunA17_Chr1g0207551 35.910 621 385 5 41 660 32 640 1.04e-131 410
Msa0005490 MtrunA17_Chr5g0441581 35.964 659 385 13 20 664 2 637 7.08e-131 404
Msa0005490 MtrunA17_Chr5g0448511 40.733 464 267 3 206 666 1 459 5.48e-129 395
Msa0005490 MtrunA17_Chr2g0296781 34.207 687 393 13 47 710 1 651 2.90e-125 391
Msa0005490 MtrunA17_Chr3g0080351 34.123 633 370 7 41 659 74 673 1.27e-121 385
Msa0005490 MtrunA17_Chr3g0118611 38.023 526 302 6 218 724 118 638 1.15e-119 378
Msa0005490 MtrunA17_Chr2g0329031 33.439 628 395 10 42 667 13 619 2.41e-119 376
Msa0005490 MtrunA17_Chr2g0287061 35.601 632 343 11 41 663 181 757 1.49e-117 373
Msa0005490 MtrunA17_Chr2g0287061 27.586 174 93 5 17 178 9 161 2.88e-15 80.1
Msa0005490 MtrunA17_Chr2g0324011 34.091 572 365 6 37 603 4 568 2.77e-114 358
Msa0005490 MtrunA17_Chr2g0291581 32.927 656 399 12 23 663 50 679 6.67e-113 357
Msa0005490 MtrunA17_Chr8g0363841 31.970 660 412 11 31 664 3 651 7.89e-113 357
Msa0005490 MtrunA17_Chr6g0477261 36.101 554 331 9 165 710 2 540 1.03e-111 353
Msa0005490 MtrunA17_Chr2g0287651 30.342 702 443 10 47 738 79 744 1.90e-105 343
Msa0005490 MtrunA17_Chr1g0198761 30.350 715 457 12 44 742 48 737 4.57e-105 339
Msa0005490 MtrunA17_Chr1g0203721 31.040 625 422 6 46 666 66 685 3.00e-104 340
Msa0005490 MtrunA17_Chr1g0196541 34.397 564 345 9 47 598 1 551 6.90e-104 335
Msa0005490 MtrunA17_Chr6g0458391 32.920 565 351 6 43 603 225 765 5.00e-102 331
Msa0005490 MtrunA17_Chr6g0458391 27.749 191 126 4 23 204 71 258 6.06e-13 72.4
Msa0005490 MtrunA17_Chr3g0082121 31.738 627 408 9 22 645 22 631 5.04e-99 321
Msa0005490 MtrunA17_Chr2g0291591 33.661 508 327 6 42 547 63 562 2.92e-94 305
Msa0005490 MtrunA17_Chr1g0203051 29.642 587 399 6 11 591 44 622 3.43e-90 296
Msa0005490 MtrunA17_Chr2g0276841 28.526 631 422 12 43 663 70 681 1.64e-80 272
Msa0005490 MtrunA17_Chr2g0294841 26.685 727 480 15 41 738 43 745 2.18e-80 273
Msa0005490 MtrunA17_Chr2g0290391 29.148 669 434 16 12 663 21 666 3.08e-76 260
Msa0005490 MtrunA17_Chr1g0164591 36.842 304 183 2 134 437 14 308 4.29e-67 225
Msa0005490 MtrunA17_Chr5g0431001 31.847 471 299 10 5 456 16 483 2.60e-65 226
Msa0005490 MtrunA17_Chr7g0258861 36.770 291 179 4 35 322 2 290 8.25e-60 204
Msa0005490 MtrunA17_Chr3g0077241 76.087 92 18 2 6 96 1 89 2.00e-39 141
Msa0005490 MtrunA17_Chr4g0052331 53.684 95 44 0 1 95 1 95 1.01e-27 107
Msa0005490 MtrunA17_Chr2g0308841 28.054 221 152 3 383 599 6 223 1.70e-24 103
Msa0005490 MtrunA17_Chr2g0329361 35.099 151 86 3 5 148 22 167 4.53e-23 99.4
Msa0005490 MtrunA17_Chr2g0329421 30.921 152 82 3 42 173 26 174 7.55e-20 89.0
Msa0005490 MtrunA17_Chr1g0149131 32.727 110 74 0 323 432 1 110 8.72e-20 85.9
Msa0005490 MtrunA17_Chr4g0033611 29.560 159 88 3 36 173 69 224 1.42e-18 86.3
Msa0005490 MtrunA17_Chr3g0078041 36.842 114 67 4 42 152 49 160 3.62e-16 78.6
Msa0005490 MtrunA17_Chr7g0233911 36.449 107 62 3 42 145 33 136 3.48e-15 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0005490 AT4G38180.1 41.568 676 370 9 2 659 21 689 0.0 539
Msa0005490 AT4G15090.1 34.816 652 408 8 37 681 45 686 2.58e-136 423
Msa0005490 AT4G15090.5 34.816 652 408 8 37 681 45 686 2.58e-136 423
Msa0005490 AT4G15090.3 34.816 652 408 8 37 681 45 686 2.58e-136 423
Msa0005490 AT4G15090.2 34.816 652 408 8 37 681 45 686 2.58e-136 423
Msa0005490 AT4G15090.4 34.816 652 408 8 37 681 45 686 2.58e-136 423
Msa0005490 AT2G27110.2 36.000 625 360 9 42 663 48 635 2.61e-128 403
Msa0005490 AT2G27110.1 36.000 625 360 9 42 663 48 635 2.61e-128 403
Msa0005490 AT3G06250.2 34.913 633 340 11 42 663 189 760 2.93e-126 395
Msa0005490 AT3G06250.2 33.962 106 54 2 41 145 26 116 1.89e-14 77.8
Msa0005490 AT3G06250.1 34.913 633 340 11 42 663 189 760 2.93e-126 395
Msa0005490 AT3G06250.1 33.962 106 54 2 41 145 26 116 1.89e-14 77.8
Msa0005490 AT3G06250.3 34.913 633 340 11 42 663 189 760 2.93e-126 395
Msa0005490 AT3G06250.3 33.962 106 54 2 41 145 26 116 1.89e-14 77.8
Msa0005490 AT5G18960.2 35.118 635 339 11 42 663 210 784 6.34e-125 392
Msa0005490 AT5G18960.2 31.132 106 57 2 41 145 41 131 3.40e-13 73.9
Msa0005490 AT5G18960.1 35.118 635 339 11 42 663 210 784 6.34e-125 392
Msa0005490 AT5G18960.1 31.132 106 57 2 41 145 41 131 3.40e-13 73.9
Msa0005490 AT2G27110.3 40.135 446 261 2 218 663 51 490 2.43e-117 370
Msa0005490 AT3G22170.2 31.024 635 393 7 39 660 66 668 2.10e-112 361
Msa0005490 AT3G22170.1 31.024 635 393 7 39 660 66 668 2.10e-112 361
Msa0005490 AT1G76320.3 32.039 618 397 8 47 662 1 597 2.61e-109 348
Msa0005490 AT1G76320.2 32.039 618 397 8 47 662 1 597 1.09e-108 348
Msa0005490 AT1G76320.1 32.039 618 397 8 47 662 1 597 1.38e-108 348
Msa0005490 AT1G76320.4 32.039 618 397 8 47 662 1 597 1.38e-108 348
Msa0005490 AT4G38170.1 36.250 480 292 6 222 698 21 489 2.56e-106 336
Msa0005490 AT1G52520.1 29.542 677 419 12 11 667 61 699 1.30e-101 328
Msa0005490 AT2G32250.5 31.200 625 373 7 46 667 41 611 5.06e-99 323
Msa0005490 AT2G32250.6 31.200 625 373 7 46 667 41 611 1.03e-98 323
Msa0005490 AT2G32250.1 31.200 625 373 7 46 667 41 611 1.03e-98 323
Msa0005490 AT2G32250.3 31.200 625 373 7 46 667 41 611 1.03e-98 323
Msa0005490 AT2G32250.2 31.200 625 373 7 46 667 41 611 1.12e-98 323
Msa0005490 AT2G32250.4 31.200 625 373 7 46 667 41 611 1.12e-98 323
Msa0005490 AT2G32250.8 31.090 624 373 7 47 667 1 570 5.25e-98 321
Msa0005490 AT2G32250.7 31.090 624 373 7 47 667 1 570 7.98e-98 320
Msa0005490 AT4G19990.3 30.920 663 394 12 41 684 19 636 9.30e-98 320
Msa0005490 AT4G19990.4 30.920 663 394 12 41 684 19 636 9.30e-98 320
Msa0005490 AT4G19990.5 30.920 663 394 12 41 684 19 636 1.41e-97 319
Msa0005490 AT4G19990.6 30.920 663 394 12 41 684 19 636 1.45e-97 320
Msa0005490 AT4G19990.2 30.920 663 394 12 41 684 19 636 1.45e-97 320
Msa0005490 AT4G19990.7 30.920 663 394 12 41 684 33 650 2.04e-97 319
Msa0005490 AT4G19990.1 31.652 575 346 9 115 684 47 579 2.64e-91 301
Msa0005490 AT1G80010.1 28.863 686 430 21 12 672 73 725 6.44e-89 295
Msa0005490 AT5G28530.1 28.774 636 416 14 43 657 55 674 1.94e-78 266
Msa0005490 AT5G28530.3 28.459 636 414 14 43 657 55 670 9.29e-77 261
Msa0005490 AT5G28530.2 28.459 636 414 14 43 657 55 670 9.29e-77 261
Msa0005490 AT1G10240.2 28.855 655 425 17 27 658 25 661 4.57e-76 259
Msa0005490 AT1G10240.1 28.855 655 425 17 27 658 25 661 4.57e-76 259
Msa0005490 AT5G28530.4 25.237 634 378 18 43 657 55 611 6.48e-50 186
Msa0005490 AT5G28530.5 24.921 634 376 18 43 657 55 607 2.29e-48 181
Msa0005490 AT3G59470.3 39.450 109 62 2 42 149 69 174 2.56e-20 91.7
Msa0005490 AT3G59470.1 39.450 109 62 2 42 149 69 174 2.56e-20 91.7
Msa0005490 AT3G59470.2 39.450 109 62 2 42 149 81 186 3.73e-20 91.7
Msa0005490 AT3G07500.1 38.318 107 63 2 41 146 31 135 9.46e-16 77.4
Msa0005490 AT2G43280.1 31.538 130 84 4 27 153 6 133 3.72e-15 75.5
Msa0005490 AT4G12850.2 34.286 105 63 2 42 145 10 109 5.62e-15 73.2
Msa0005490 AT4G12850.4 31.746 126 78 3 42 164 10 130 6.88e-15 72.8
Msa0005490 AT4G12850.1 35.238 105 62 2 42 145 10 109 9.97e-15 73.6
Msa0005490 AT2G43280.2 32.323 99 62 4 58 153 2 98 6.87e-11 62.0

Find 153 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTCTGCAATAAATATT+TGG 0.060470 1_1:-8159408 None:intergenic
TGCACTTCTGGCGTGATTTC+AGG 0.168073 1_1:+8160443 Msa0005490:three_prime_UTR
AGAATGGCTTACAAATATTT+TGG 0.184037 1_1:+8158623 Msa0005490:CDS
TCGGCAAAATTATCCCTTAT+TGG 0.193753 1_1:-8160376 None:intergenic
TTATACAACTTTACTATAAA+TGG 0.200880 1_1:-8160803 None:intergenic
GATTTCTCTGATGTGATTCT+AGG 0.204971 1_1:+8160413 Msa0005490:three_prime_UTR
TCTACACCATTCGAGGAAAA+TGG 0.218597 1_1:+8159095 Msa0005490:CDS
TTCATTATTTAATAGTATTA+TGG 0.222727 1_1:+8159723 Msa0005490:CDS
CTAGGCTTTGGATGCACTTC+TGG 0.230580 1_1:+8160431 Msa0005490:three_prime_UTR
GTAAATTAGCAAATTGCTTT+TGG 0.235893 1_1:+8158585 Msa0005490:CDS
AAAAGTGTTCAGGATATTTC+AGG 0.242179 1_1:+8159369 Msa0005490:CDS
ATTGATGTGCTTCATGATTC+TGG 0.246659 1_1:+8158350 Msa0005490:CDS
CATTCCTTCAAAGGGCTCTA+AGG 0.261818 1_1:-8157967 None:intergenic
TATTCATACTTTCACTCCTT+TGG 0.266644 1_1:-8159165 None:intergenic
CGCTATGTTCATGATAGATT+TGG 0.274190 1_1:-8158952 None:intergenic
ATTCATACTTTCACTCCTTT+GGG 0.296462 1_1:-8159164 None:intergenic
CTGATGACTTTGATTCTGAT+TGG 0.300769 1_1:+8159020 Msa0005490:CDS
TCTGGCGTGATTTCAGGTTT+TGG 0.301040 1_1:+8160449 Msa0005490:three_prime_UTR
AAAGTGCGTGGACGACCTTT+AGG 0.302776 1_1:-8159952 None:intergenic
TGATGACTTTGATTCTGATT+GGG 0.310601 1_1:+8159021 Msa0005490:CDS
ATATTGCAATTCAAGCTTTA+CGG 0.318900 1_1:+8159797 Msa0005490:CDS
ATCCTTCCATTTCTTATTGA+AGG 0.322059 1_1:+8157285 Msa0005490:five_prime_UTR
CGAAGATGCTGTGAACTTCT+TGG 0.326106 1_1:-8158290 None:intergenic
TTTCTCTGCAATAAATATTT+GGG 0.327509 1_1:-8159407 None:intergenic
TCTGATGTGATTCTAGGCTT+TGG 0.327651 1_1:+8160419 Msa0005490:three_prime_UTR
AATAATTAATAACATCTTGT+TGG 0.331163 1_1:-8158442 None:intergenic
TGAAAAGTCACATGATAGTT+AGG 0.341747 1_1:-8159487 None:intergenic
GAAGATGCTGTGAACTTCTT+GGG 0.347598 1_1:-8158289 None:intergenic
TCCATTGCAATAACTACAAC+TGG 0.351484 1_1:-8160016 None:intergenic
TGGAGGATCACACAAGTTTA+TGG 0.352054 1_1:-8159920 None:intergenic
AAGTGCGTGGACGACCTTTA+GGG 0.355019 1_1:-8159951 None:intergenic
ATGCCCTTTGTTCCATTCAC+TGG 0.364662 1_1:+8158692 Msa0005490:CDS
CAATGTTAAAGCTTCTAGTT+TGG 0.368597 1_1:+8160214 Msa0005490:CDS
TATTTATTTGTTAAGTATGT+TGG 0.372145 1_1:-8158665 None:intergenic
TTTACGAATGAAAGGCAGTT+TGG 0.374955 1_1:+8159972 Msa0005490:CDS
TTCTAGTTTGGTTGATTAAG+AGG 0.375412 1_1:+8160226 Msa0005490:exon
TTTATACAAGAAAAGTGTTC+AGG 0.375460 1_1:+8159359 Msa0005490:CDS
GATTATGGTCTCTTACAAAT+CGG 0.380412 1_1:-8158253 None:intergenic
GATTTATCAAACTCTTCATT+TGG 0.382353 1_1:-8159859 None:intergenic
TTGTCGCATGTTTGAGTTTG+TGG 0.384532 1_1:+8159537 Msa0005490:CDS
AATTGTCAGTTGAAGAATCC+TGG 0.396913 1_1:+8158530 Msa0005490:CDS
TCCATACCTTCAATAAGAAA+TGG 0.399424 1_1:-8157291 None:intergenic
TGTATTAAACGTATTCATAA+AGG 0.409882 1_1:+8159576 Msa0005490:CDS
GGGAAAATAGTACATGATTA+TGG 0.411615 1_1:-8158268 None:intergenic
AATCACAAAACTTGATCAAT+TGG 0.412422 1_1:+8159825 Msa0005490:CDS
TACCTTTGGTGCGGCTGTGC+GGG 0.417771 1_1:+8160529 Msa0005490:three_prime_UTR
TGAATGGAACAAAGGGCATT+AGG 0.418217 1_1:-8158688 None:intergenic
CGCACCAAAGGTACAAAAGC+TGG 0.418500 1_1:-8160519 None:intergenic
TTGTGAAAATGAAGGAGAAC+AGG 0.419556 1_1:-8157214 None:intergenic
TTGTTTGGTTGCTACGTACT+TGG 0.423904 1_1:+8158783 Msa0005490:CDS
AGTCATCAGAAGTGATAGTT+TGG 0.427220 1_1:-8159006 None:intergenic
GCAACAGAGAGTTGCAGATT+CGG 0.435446 1_1:-8157262 None:intergenic
TGCATCAATTGAAGATGTAA+AGG 0.436857 1_1:+8157995 Msa0005490:CDS
GTGCTTCATGATTCTGGTAT+AGG 0.438869 1_1:+8158356 Msa0005490:CDS
ACTTTCACTCCTTTGGGTGG+TGG 0.440297 1_1:-8159158 None:intergenic
TGTAGTTATTGCAATGGAAA+AGG 0.442086 1_1:+8160021 Msa0005490:CDS
GGCTCTAAGGGGAGCTCTGA+TGG 0.445219 1_1:-8157954 None:intergenic
TACATCATCTGAGCCATTCA+TGG 0.452459 1_1:-8157889 None:intergenic
CATCTACATAACAGGATTGT+GGG 0.453044 1_1:+8157819 Msa0005490:intron
TTTAGGTGTGATATCATCAA+TGG 0.453788 1_1:+8160176 Msa0005490:intron
CATGAACATACCACCATGAA+TGG 0.455856 1_1:+8157876 Msa0005490:CDS
CTCCCCTTAGAGCCCTTTGA+AGG 0.456460 1_1:+8157963 Msa0005490:CDS
GGTTGATTCAAGGTCTAGAA+TGG 0.460592 1_1:+8158607 Msa0005490:CDS
TATTTCAGGATGAGTTGGTT+GGG 0.463690 1_1:+8159383 Msa0005490:CDS
AAGAAGATCTACACCATTCG+AGG 0.468875 1_1:+8159088 Msa0005490:CDS
CTACACCATTCGAGGAAAAT+GGG 0.470377 1_1:+8159096 Msa0005490:CDS
AGTGGTGGTGTTGATAATGT+TGG 0.474266 1_1:+8158413 Msa0005490:CDS
ATTATGGTCTCTTACAAATC+GGG 0.474650 1_1:-8158252 None:intergenic
CTTAGAGCCCTTTGAAGGAA+TGG 0.476103 1_1:+8157968 Msa0005490:CDS
GATGACTTTGATTCTGATTG+GGG 0.477884 1_1:+8159022 Msa0005490:CDS
ACCAGTTGTAGTTATTGCAA+TGG 0.478462 1_1:+8160015 Msa0005490:CDS
CAGGATATTTCAGGATGAGT+TGG 0.484717 1_1:+8159378 Msa0005490:CDS
TGCAAACTCCATTCCTTCAA+AGG 0.485592 1_1:-8157976 None:intergenic
TGTCGCATGTTTGAGTTTGT+GGG 0.487042 1_1:+8159538 Msa0005490:CDS
CAAACTAGAAGCTTTAACAT+TGG 0.487651 1_1:-8160213 None:intergenic
AAATCCACCACCTTTGACTT+CGG 0.488240 1_1:-8160395 None:intergenic
ATTCCTTCAAAGGGCTCTAA+GGG 0.488264 1_1:-8157966 None:intergenic
CTCAATATGTTCTAAGCCGA+TGG 0.491066 1_1:+8159620 Msa0005490:CDS
TCTTAGCATTTATGGTCCAT+CGG 0.492991 1_1:-8159636 None:intergenic
TTAAAGTAAAATGTTAAAAG+AGG 0.495012 1_1:-8160694 None:intergenic
ACGAGTTGCAAGATAACAAA+TGG 0.498356 1_1:+8159062 Msa0005490:CDS
ATTGTCAGTTGAAGAATCCT+GGG 0.500058 1_1:+8158531 Msa0005490:CDS
TAGATTTGGTTCAAGTACTC+TGG 0.502715 1_1:-8158938 None:intergenic
TTACTAGGAGGGGATCTGTT+CGG 0.504637 1_1:+8160580 Msa0005490:three_prime_UTR
TTTGAAGCCTCTTCTTCCAT+GGG 0.505212 1_1:-8159334 None:intergenic
TCAAGAGATTCTTTGTTCAA+AGG 0.507548 1_1:+8158106 Msa0005490:CDS
GCAAACTCCATTCCTTCAAA+GGG 0.507732 1_1:-8157975 None:intergenic
TTTGCCGAAGTCAAAGGTGG+TGG 0.512830 1_1:+8160391 Msa0005490:three_prime_UTR
CAATAATGATGATTCACCTC+TGG 0.513933 1_1:-8158890 None:intergenic
TCAGATTGTGTTTACTAGGA+GGG 0.514506 1_1:+8160569 Msa0005490:three_prime_UTR
AAACTCTGACGTACATAAGA+AGG 0.521777 1_1:-8159121 None:intergenic
AGAACGAATGGTGTTGTGAT+TGG 0.522138 1_1:+8158083 Msa0005490:CDS
CCTCCACGTGCGGTCCCTAA+AGG 0.522617 1_1:+8159937 Msa0005490:CDS
TGTATCACAAACTTGCTCAA+TGG 0.525322 1_1:-8159220 None:intergenic
GTCTGGTCATCGGAGACTTT+AGG 0.525728 1_1:-8159898 None:intergenic
CATACTTTCACTCCTTTGGG+TGG 0.526942 1_1:-8159161 None:intergenic
TCTGCGTACTTTATATCCCA+TGG 0.529545 1_1:+8159318 Msa0005490:CDS
ACTCCAGTGAATGGAACAAA+GGG 0.535906 1_1:-8158695 None:intergenic
ACGTACTTGGTTAGAAGCAA+TGG 0.537041 1_1:+8158796 Msa0005490:CDS
CAGATTGTGTTTACTAGGAG+GGG 0.539634 1_1:+8160570 Msa0005490:three_prime_UTR
TCTCTTGAACTATCTGAAAG+AGG 0.540310 1_1:+8159751 Msa0005490:CDS
AGAGATTCTTTGTTCAAAGG+AGG 0.541828 1_1:+8158109 Msa0005490:CDS
TTTATATCCCATGGAAGAAG+AGG 0.541904 1_1:+8159327 Msa0005490:CDS
CAAGTTTATGGTCTGGTCAT+CGG 0.542082 1_1:-8159908 None:intergenic
TGAATCATCATTATTGTATG+TGG 0.542391 1_1:+8158897 Msa0005490:CDS
TTGGACATCTACGTTTGTTG+TGG 0.543214 1_1:-8160044 None:intergenic
TGCAATTCAAGCTTTACGGA+AGG 0.544992 1_1:+8159801 Msa0005490:CDS
ATATTTCAGGATGAGTTGGT+TGG 0.547184 1_1:+8159382 Msa0005490:CDS
GTACCTTTGGTGCGGCTGTG+CGG 0.547232 1_1:+8160528 Msa0005490:three_prime_UTR
ACATCTACATAACAGGATTG+TGG 0.547572 1_1:+8157818 Msa0005490:intron
GAGATTCTTTGTTCAAAGGA+GGG 0.548544 1_1:+8158110 Msa0005490:CDS
TATTTCTAATGCAGTTGCAA+AGG 0.550472 1_1:+8158862 Msa0005490:CDS
GATCACACAAGTTTATGGTC+TGG 0.557810 1_1:-8159915 None:intergenic
TTCAGATTGTGTTTACTAGG+AGG 0.562996 1_1:+8160568 Msa0005490:three_prime_UTR
GCACCAAAGGTACAAAAGCT+GGG 0.568721 1_1:-8160518 None:intergenic
CGGCTTAGAACATATTGAGA+AGG 0.582615 1_1:-8159616 None:intergenic
GTCCAAGTTTAAAACATGGA+AGG 0.582831 1_1:+8160061 Msa0005490:CDS
AACTCCAGTGAATGGAACAA+AGG 0.585483 1_1:-8158696 None:intergenic
TGATGATTAACTCCAGTGAA+TGG 0.587906 1_1:-8158704 None:intergenic
TTAAAGTACACGATCAAACA+AGG 0.591318 1_1:+8158174 Msa0005490:CDS
GTTACTAAATCAAGAACGAA+TGG 0.591678 1_1:+8158071 Msa0005490:CDS
AAGATGCTGTGAACTTCTTG+GGG 0.593260 1_1:-8158288 None:intergenic
ATGTAAAGGATTACTACGTA+AGG 0.595028 1_1:+8158009 Msa0005490:CDS
GGCTTAGAACATATTGAGAA+GGG 0.595706 1_1:-8159615 None:intergenic
TACCTTCAATAAGAAATGGA+AGG 0.597326 1_1:-8157287 None:intergenic
GCATTTCAAATGGACGCAGA+AGG 0.597955 1_1:+8158563 Msa0005490:CDS
CCACGCACTTTACGAATGAA+AGG 0.600807 1_1:+8159964 Msa0005490:CDS
TCTAAGGGGAGCTCTGATGG+CGG 0.601475 1_1:-8157951 None:intergenic
GCAATTGAAGACCTCGAGGA+AGG 0.603885 1_1:+8159679 Msa0005490:CDS
CCTTTCATTCGTAAAGTGCG+TGG 0.604290 1_1:-8159964 None:intergenic
GGATATAAAGTACGCAGAAG+TGG 0.606925 1_1:-8159313 None:intergenic
CTCTGTTTCTGCCTTCCTCG+AGG 0.612225 1_1:-8159690 None:intergenic
AACCCGCACAGCCGCACCAA+AGG 0.615562 1_1:-8160531 None:intergenic
CAGAGCGGTTGTGAAAATGA+AGG 0.616006 1_1:-8157222 None:intergenic
GAGAGAAGAATGTCAAGACA+AGG 0.616684 1_1:+8159281 Msa0005490:CDS
GAACAGGGTAACTGATGTTG+AGG 0.619611 1_1:-8157198 None:intergenic
CGACCTTTAGGGACCGCACG+TGG 0.624526 1_1:-8159940 None:intergenic
GTTTGTGATACAGTATGAGA+AGG 0.624921 1_1:+8159231 Msa0005490:CDS
GTACACGATCAAACAAGGGT+AGG 0.637469 1_1:+8158179 Msa0005490:CDS
CTGGAATGTCCACCACCCAA+AGG 0.639739 1_1:+8159149 Msa0005490:CDS
AGATGTCCAAGTTTAAAACA+TGG 0.643966 1_1:+8160057 Msa0005490:CDS
ACTTGGTTAGAAGCAATGGA+TGG 0.652582 1_1:+8158800 Msa0005490:CDS
TCTTGACGCACGTTATAACA+AGG 0.672022 1_1:+8159255 Msa0005490:CDS
TTCCTTCAAAGGGCTCTAAG+GGG 0.687755 1_1:-8157965 None:intergenic
TGTGAAAATGAAGGAGAACA+GGG 0.696875 1_1:-8157213 None:intergenic
CCTTTAGGGACCGCACGTGG+AGG 0.703909 1_1:-8159937 None:intergenic
ATCATCTGAGCCATTCATGG+TGG 0.705037 1_1:-8157886 None:intergenic
AATTGCAATTGAAGACCTCG+AGG 0.709068 1_1:+8159675 Msa0005490:CDS
CTTGTGTGATCCTCCACGTG+CGG 0.710705 1_1:+8159927 Msa0005490:CDS
TAAAGTACACGATCAAACAA+GGG 0.712268 1_1:+8158175 Msa0005490:CDS
GTGTTATGTACTGAGAGTGG+TGG 0.725945 1_1:+8158398 Msa0005490:CDS
TCAGTGTTATGTACTGAGAG+TGG 0.733515 1_1:+8158395 Msa0005490:CDS
GTCGAGAATCAAGTACAGAG+CGG 0.751791 1_1:-8157237 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATATAAGTAAAAAAG+TGG - chr1_1:8157745-8157764 None:intergenic 10.0%
!!! TTCTTTTTTCAATTTTTTTT+TGG - chr1_1:8158144-8158163 None:intergenic 10.0%
!! AAAAAATTGAAAAAAGAAAA+TGG + chr1_1:8158146-8158165 Msa0005490:CDS 10.0%
!!! AAGTATGAATAAATTTTTTA+AGG + chr1_1:8159177-8159196 Msa0005490:CDS 10.0%
!!! AGTATGAATAAATTTTTTAA+GGG + chr1_1:8159178-8159197 Msa0005490:CDS 10.0%
!! TTCATTATTTAATAGTATTA+TGG + chr1_1:8159723-8159742 Msa0005490:CDS 10.0%
!!! TAACATTTTACTTTAATATA+AGG + chr1_1:8160701-8160720 Msa0005490:three_prime_UTR 10.0%
!! ACAATAATATGCTATTATAA+AGG - chr1_1:8157691-8157710 None:intergenic 15.0%
!! CATATATATAAGTAAAAAAG+TGG - chr1_1:8157777-8157796 None:intergenic 15.0%
!! AATAATTAATAACATCTTGT+TGG - chr1_1:8158445-8158464 None:intergenic 15.0%
!!! TATTTATTTGTTAAGTATGT+TGG - chr1_1:8158668-8158687 None:intergenic 15.0%
!! CTTGTAACAATAATATATTT+AGG - chr1_1:8160301-8160320 None:intergenic 15.0%
!!! CTTTTATTGTTTTTCTTTTT+AGG + chr1_1:8160658-8160677 Msa0005490:three_prime_UTR 15.0%
!! TTAAAGTAAAATGTTAAAAG+AGG - chr1_1:8160697-8160716 None:intergenic 15.0%
!!! TATTCACAATCTTTGTTTTT+TGG + chr1_1:8157333-8157352 Msa0005490:intron 20.0%
!! TGAAAAAACCATAATAATAG+AGG - chr1_1:8157541-8157560 None:intergenic 20.0%
!!! AAAACTAAAACCTTTTTTCT+TGG - chr1_1:8158039-8158058 None:intergenic 20.0%
!! AGACAAAAACAATTAGAAAA+TGG + chr1_1:8158476-8158495 Msa0005490:CDS 20.0%
!!! GTTTTTTTATGCATTTCAAA+TGG + chr1_1:8158553-8158572 Msa0005490:CDS 20.0%
!!! TAAATTAGCAAATTGCTTTT+GGG + chr1_1:8158586-8158605 Msa0005490:CDS 20.0%
!! TTTCTCTGCAATAAATATTT+GGG - chr1_1:8159410-8159429 None:intergenic 20.0%
!!! AATTTCATGAATTTTGTATG+TGG - chr1_1:8159455-8159474 None:intergenic 20.0%
!! TGTATTAAACGTATTCATAA+AGG + chr1_1:8159576-8159595 Msa0005490:CDS 20.0%
!!! TTTTTCTTTCTTAGCATTTA+TGG - chr1_1:8159647-8159666 None:intergenic 20.0%
!!! TATTGTTGTTATTGTTTTAC+TGG + chr1_1:8160249-8160268 Msa0005490:intron 20.0%
! CAAATATCTAAAAGCTTATC+AGG - chr1_1:8157466-8157485 None:intergenic 25.0%
! TACATCAAGAAAAATGAAGA+TGG + chr1_1:8158218-8158237 Msa0005490:CDS 25.0%
! ACATCAAGAAAAATGAAGAT+GGG + chr1_1:8158219-8158238 Msa0005490:CDS 25.0%
!!! AATAAGTTTTTTTTGCACTG+AGG - chr1_1:8158333-8158352 None:intergenic 25.0%
!! GTAAATTAGCAAATTGCTTT+TGG + chr1_1:8158585-8158604 Msa0005490:CDS 25.0%
!! AGAATGGCTTACAAATATTT+TGG + chr1_1:8158623-8158642 Msa0005490:CDS 25.0%
!!! TTACAAATATTTTGGTGATG+TGG + chr1_1:8158631-8158650 Msa0005490:CDS 25.0%
!! AACCAAAAAGTATAGATTGT+TGG - chr1_1:8158730-8158749 None:intergenic 25.0%
!! TGATCAGTGATAATAGTTTT+AGG - chr1_1:8158833-8158852 None:intergenic 25.0%
! TGAATCATCATTATTGTATG+TGG + chr1_1:8158897-8158916 Msa0005490:CDS 25.0%
!!! TTTATACAAGAAAAGTGTTC+AGG + chr1_1:8159359-8159378 Msa0005490:CDS 25.0%
! CTTTCTCTGCAATAAATATT+TGG - chr1_1:8159411-8159430 None:intergenic 25.0%
! GCTAAGAAAGAAAAAAGTAA+AGG + chr1_1:8159652-8159671 Msa0005490:CDS 25.0%
! ATATTGCAATTCAAGCTTTA+CGG + chr1_1:8159797-8159816 Msa0005490:CDS 25.0%
! AATCACAAAACTTGATCAAT+TGG + chr1_1:8159825-8159844 Msa0005490:CDS 25.0%
! GATTTATCAAACTCTTCATT+TGG - chr1_1:8159862-8159881 None:intergenic 25.0%
!!! TGAATTTCCATCTATTTTTC+TGG + chr1_1:8160486-8160505 Msa0005490:three_prime_UTR 25.0%
!!! GGTTTTGATATTTGATTTGT+AGG + chr1_1:8160601-8160620 Msa0005490:three_prime_UTR 25.0%
ATCCTTCCATTTCTTATTGA+AGG + chr1_1:8157285-8157304 Msa0005490:five_prime_UTR 30.0%
TACCTTCAATAAGAAATGGA+AGG - chr1_1:8157290-8157309 None:intergenic 30.0%
TCCATTTCTTATTGAAGGTA+TGG + chr1_1:8157290-8157309 Msa0005490:intron 30.0%
TCCATACCTTCAATAAGAAA+TGG - chr1_1:8157294-8157313 None:intergenic 30.0%
AACAAAGATTGTGAATAGAG+TGG - chr1_1:8157330-8157349 None:intergenic 30.0%
TCACAGTTACTCATCTATTA+TGG + chr1_1:8157396-8157415 Msa0005490:intron 30.0%
TCATCATACATCTACATAAC+AGG + chr1_1:8157811-8157830 Msa0005490:intron 30.0%
TGCATCAATTGAAGATGTAA+AGG + chr1_1:8157995-8158014 Msa0005490:CDS 30.0%
ATGTAAAGGATTACTACGTA+AGG + chr1_1:8158009-8158028 Msa0005490:CDS 30.0%
!!! AAAAGGTTTTAGTTTTCGCA+TGG + chr1_1:8158043-8158062 Msa0005490:CDS 30.0%
!!! AAAGGTTTTAGTTTTCGCAT+GGG + chr1_1:8158044-8158063 Msa0005490:CDS 30.0%
GTTACTAAATCAAGAACGAA+TGG + chr1_1:8158071-8158090 Msa0005490:CDS 30.0%
TCAAGAGATTCTTTGTTCAA+AGG + chr1_1:8158106-8158125 Msa0005490:CDS 30.0%
TTAAAGTACACGATCAAACA+AGG + chr1_1:8158174-8158193 Msa0005490:CDS 30.0%
TAAAGTACACGATCAAACAA+GGG + chr1_1:8158175-8158194 Msa0005490:CDS 30.0%
ATTATGGTCTCTTACAAATC+GGG - chr1_1:8158255-8158274 None:intergenic 30.0%
GATTATGGTCTCTTACAAAT+CGG - chr1_1:8158256-8158275 None:intergenic 30.0%
GGGAAAATAGTACATGATTA+TGG - chr1_1:8158271-8158290 None:intergenic 30.0%
!!! ACACTGATGCTATTTTACTA+GGG - chr1_1:8158382-8158401 None:intergenic 30.0%
!!! AACACTGATGCTATTTTACT+AGG - chr1_1:8158383-8158402 None:intergenic 30.0%
TGAAATGCATAAAAAAACCC+AGG - chr1_1:8158551-8158570 None:intergenic 30.0%
! CACCAACAATCTATACTTTT+TGG + chr1_1:8158725-8158744 Msa0005490:CDS 30.0%
!!! AAACTGAAGAGAGTTTTGTT+TGG + chr1_1:8158768-8158787 Msa0005490:CDS 30.0%
! TATTTCTAATGCAGTTGCAA+AGG + chr1_1:8158862-8158881 Msa0005490:CDS 30.0%
TGATGACTTTGATTCTGATT+GGG + chr1_1:8159021-8159040 Msa0005490:CDS 30.0%
ATTCATACTTTCACTCCTTT+GGG - chr1_1:8159167-8159186 None:intergenic 30.0%
TATTCATACTTTCACTCCTT+TGG - chr1_1:8159168-8159187 None:intergenic 30.0%
!! AAAAGTGTTCAGGATATTTC+AGG + chr1_1:8159369-8159388 Msa0005490:CDS 30.0%
TGAAAAGTCACATGATAGTT+AGG - chr1_1:8159490-8159509 None:intergenic 30.0%
TGTAGTTATTGCAATGGAAA+AGG + chr1_1:8160021-8160040 Msa0005490:CDS 30.0%
! AGATGTCCAAGTTTAAAACA+TGG + chr1_1:8160057-8160076 Msa0005490:CDS 30.0%
!! TACCTTCCATGTTTTAAACT+TGG - chr1_1:8160066-8160085 None:intergenic 30.0%
GGTTTGTAACTGAATAAACT+TGG - chr1_1:8160149-8160168 None:intergenic 30.0%
! GTTACAAACCAATGTCTTTT+AGG + chr1_1:8160159-8160178 Msa0005490:intron 30.0%
ATATCACACCTAAAAGACAT+TGG - chr1_1:8160170-8160189 None:intergenic 30.0%
TTTAGGTGTGATATCATCAA+TGG + chr1_1:8160176-8160195 Msa0005490:intron 30.0%
! CAAACTAGAAGCTTTAACAT+TGG - chr1_1:8160216-8160235 None:intergenic 30.0%
! CAATGTTAAAGCTTCTAGTT+TGG + chr1_1:8160214-8160233 Msa0005490:CDS 30.0%
!! TTCTAGTTTGGTTGATTAAG+AGG + chr1_1:8160226-8160245 Msa0005490:exon 30.0%
CTAGTGTTAATTCCTAGTAT+TGG + chr1_1:8160332-8160351 Msa0005490:intron 30.0%
!! ATTTCCATCTATTTTTCTGG+TGG + chr1_1:8160489-8160508 Msa0005490:three_prime_UTR 30.0%
!! TTTCCATCTATTTTTCTGGT+GGG + chr1_1:8160490-8160509 Msa0005490:three_prime_UTR 30.0%
! CTTTTCAGATTGTGTTTACT+AGG + chr1_1:8160565-8160584 Msa0005490:three_prime_UTR 30.0%
TGTGAAAATGAAGGAGAACA+GGG - chr1_1:8157216-8157235 None:intergenic 35.0%
TTGTGAAAATGAAGGAGAAC+AGG - chr1_1:8157217-8157236 None:intergenic 35.0%
! CCATAATAATAGAGGTGCAT+GGG - chr1_1:8157533-8157552 None:intergenic 35.0%
! ACCATAATAATAGAGGTGCA+TGG - chr1_1:8157534-8157553 None:intergenic 35.0%
ACAACCAGCAAACTAAAGAT+TGG - chr1_1:8157621-8157640 None:intergenic 35.0%
ACATCTACATAACAGGATTG+TGG + chr1_1:8157818-8157837 Msa0005490:intron 35.0%
CATCTACATAACAGGATTGT+GGG + chr1_1:8157819-8157838 Msa0005490:intron 35.0%
AGAGATTCTTTGTTCAAAGG+AGG + chr1_1:8158109-8158128 Msa0005490:CDS 35.0%
GAGATTCTTTGTTCAAAGGA+GGG + chr1_1:8158110-8158129 Msa0005490:CDS 35.0%
TCAAGAAAAATGAAGATGGG+TGG + chr1_1:8158222-8158241 Msa0005490:CDS 35.0%
ATTGATGTGCTTCATGATTC+TGG + chr1_1:8158350-8158369 Msa0005490:CDS 35.0%
AATTGTCAGTTGAAGAATCC+TGG + chr1_1:8158530-8158549 Msa0005490:CDS 35.0%
ATTGTCAGTTGAAGAATCCT+GGG + chr1_1:8158531-8158550 Msa0005490:CDS 35.0%
!!! ATTGCTTTTGGGTTGATTCA+AGG + chr1_1:8158597-8158616 Msa0005490:CDS 35.0%
TGATGATTAACTCCAGTGAA+TGG - chr1_1:8158707-8158726 None:intergenic 35.0%
!!! TTTTTGGTTGTGCTCTGTTA+TGG + chr1_1:8158741-8158760 Msa0005490:CDS 35.0%
!! TTTTGGTTGTGCTCTGTTAT+GGG + chr1_1:8158742-8158761 Msa0005490:CDS 35.0%
CAATAATGATGATTCACCTC+TGG - chr1_1:8158893-8158912 None:intergenic 35.0%
! TAGATTTGGTTCAAGTACTC+TGG - chr1_1:8158941-8158960 None:intergenic 35.0%
CGCTATGTTCATGATAGATT+TGG - chr1_1:8158955-8158974 None:intergenic 35.0%
AGTCATCAGAAGTGATAGTT+TGG - chr1_1:8159009-8159028 None:intergenic 35.0%
CTGATGACTTTGATTCTGAT+TGG + chr1_1:8159020-8159039 Msa0005490:CDS 35.0%
GATGACTTTGATTCTGATTG+GGG + chr1_1:8159022-8159041 Msa0005490:CDS 35.0%
ACGAGTTGCAAGATAACAAA+TGG + chr1_1:8159062-8159081 Msa0005490:CDS 35.0%
AAACTCTGACGTACATAAGA+AGG - chr1_1:8159124-8159143 None:intergenic 35.0%
! TGTATCACAAACTTGCTCAA+TGG - chr1_1:8159223-8159242 None:intergenic 35.0%
GTTTGTGATACAGTATGAGA+AGG + chr1_1:8159231-8159250 Msa0005490:CDS 35.0%
TTTATATCCCATGGAAGAAG+AGG + chr1_1:8159327-8159346 Msa0005490:CDS 35.0%
!! ATATTTCAGGATGAGTTGGT+TGG + chr1_1:8159382-8159401 Msa0005490:CDS 35.0%
!! TATTTCAGGATGAGTTGGTT+GGG + chr1_1:8159383-8159402 Msa0005490:CDS 35.0%
GGCTTAGAACATATTGAGAA+GGG - chr1_1:8159618-8159637 None:intergenic 35.0%
TCTTAGCATTTATGGTCCAT+CGG - chr1_1:8159639-8159658 None:intergenic 35.0%
TCTCTTGAACTATCTGAAAG+AGG + chr1_1:8159751-8159770 Msa0005490:CDS 35.0%
TTTACGAATGAAAGGCAGTT+TGG + chr1_1:8159972-8159991 Msa0005490:CDS 35.0%
ACCAGTTGTAGTTATTGCAA+TGG + chr1_1:8160015-8160034 Msa0005490:CDS 35.0%
TCCATTGCAATAACTACAAC+TGG - chr1_1:8160019-8160038 None:intergenic 35.0%
! GTCCAAGTTTAAAACATGGA+AGG + chr1_1:8160061-8160080 Msa0005490:CDS 35.0%
GTAACTGAATAAACTTGGCA+TGG - chr1_1:8160144-8160163 None:intergenic 35.0%
!!! AGACCTGTTTTTGCCAATAA+GGG + chr1_1:8160363-8160382 Msa0005490:intron 35.0%
TATCCCTTATTGGCAAAAAC+AGG - chr1_1:8160369-8160388 None:intergenic 35.0%
TCGGCAAAATTATCCCTTAT+TGG - chr1_1:8160379-8160398 None:intergenic 35.0%
! GATAATTTTGCCGAAGTCAA+AGG + chr1_1:8160385-8160404 Msa0005490:three_prime_UTR 35.0%
GATTTCTCTGATGTGATTCT+AGG + chr1_1:8160413-8160432 Msa0005490:three_prime_UTR 35.0%
ATTCCCACCAGAAAAATAGA+TGG - chr1_1:8160496-8160515 None:intergenic 35.0%
TTCAGATTGTGTTTACTAGG+AGG + chr1_1:8160568-8160587 Msa0005490:three_prime_UTR 35.0%
TCAGATTGTGTTTACTAGGA+GGG + chr1_1:8160569-8160588 Msa0005490:three_prime_UTR 35.0%
! AGTGATGGGAATTTGATACG+TGG - chr1_1:8157369-8157388 None:intergenic 40.0%
ACTGTGAAGAACTGAGTGAT+GGG - chr1_1:8157383-8157402 None:intergenic 40.0%
AACTGTGAAGAACTGAGTGA+TGG - chr1_1:8157384-8157403 None:intergenic 40.0%
! AATAATAGAGGTGCATGGGA+TGG - chr1_1:8157529-8157548 None:intergenic 40.0%
CCCATGCACCTCTATTATTA+TGG + chr1_1:8157530-8157549 Msa0005490:intron 40.0%
!! ACCTCCAATCTTTAGTTTGC+TGG + chr1_1:8157614-8157633 Msa0005490:intron 40.0%
ACCAGCAAACTAAAGATTGG+AGG - chr1_1:8157618-8157637 None:intergenic 40.0%
!! TTTAGTTTGCTGGTTGTCGT+GGG + chr1_1:8157624-8157643 Msa0005490:intron 40.0%
CATGAACATACCACCATGAA+TGG + chr1_1:8157876-8157895 Msa0005490:CDS 40.0%
TACATCATCTGAGCCATTCA+TGG - chr1_1:8157892-8157911 None:intergenic 40.0%
! ATTCCTTCAAAGGGCTCTAA+GGG - chr1_1:8157969-8157988 None:intergenic 40.0%
GCAAACTCCATTCCTTCAAA+GGG - chr1_1:8157978-8157997 None:intergenic 40.0%
TGCAAACTCCATTCCTTCAA+AGG - chr1_1:8157979-8157998 None:intergenic 40.0%
GTAAGGTACGCCAAGAAAAA+AGG + chr1_1:8158026-8158045 Msa0005490:CDS 40.0%
!! AGAACGAATGGTGTTGTGAT+TGG + chr1_1:8158083-8158102 Msa0005490:CDS 40.0%
AAGATGCTGTGAACTTCTTG+GGG - chr1_1:8158291-8158310 None:intergenic 40.0%
GAAGATGCTGTGAACTTCTT+GGG - chr1_1:8158292-8158311 None:intergenic 40.0%
GTGCTTCATGATTCTGGTAT+AGG + chr1_1:8158356-8158375 Msa0005490:CDS 40.0%
TCAGTGTTATGTACTGAGAG+TGG + chr1_1:8158395-8158414 Msa0005490:CDS 40.0%
!! AGTGGTGGTGTTGATAATGT+TGG + chr1_1:8158413-8158432 Msa0005490:CDS 40.0%
! GGTTGATTCAAGGTCTAGAA+TGG + chr1_1:8158607-8158626 Msa0005490:CDS 40.0%
!! TGAATGGAACAAAGGGCATT+AGG - chr1_1:8158691-8158710 None:intergenic 40.0%
ACTCCAGTGAATGGAACAAA+GGG - chr1_1:8158698-8158717 None:intergenic 40.0%
AACTCCAGTGAATGGAACAA+AGG - chr1_1:8158699-8158718 None:intergenic 40.0%
TTGTTTGGTTGCTACGTACT+TGG + chr1_1:8158783-8158802 Msa0005490:CDS 40.0%
ACGTACTTGGTTAGAAGCAA+TGG + chr1_1:8158796-8158815 Msa0005490:CDS 40.0%
ACTTGGTTAGAAGCAATGGA+TGG + chr1_1:8158800-8158819 Msa0005490:CDS 40.0%
! AGTTGCAAAGGTTTTTCCAG+AGG + chr1_1:8158874-8158893 Msa0005490:CDS 40.0%
AAGAAGATCTACACCATTCG+AGG + chr1_1:8159088-8159107 Msa0005490:CDS 40.0%
TCTACACCATTCGAGGAAAA+TGG + chr1_1:8159095-8159114 Msa0005490:CDS 40.0%
CTACACCATTCGAGGAAAAT+GGG + chr1_1:8159096-8159115 Msa0005490:CDS 40.0%
TCTTGACGCACGTTATAACA+AGG + chr1_1:8159255-8159274 Msa0005490:CDS 40.0%
GAGAGAAGAATGTCAAGACA+AGG + chr1_1:8159281-8159300 Msa0005490:CDS 40.0%
GGATATAAAGTACGCAGAAG+TGG - chr1_1:8159316-8159335 None:intergenic 40.0%
TCTGCGTACTTTATATCCCA+TGG + chr1_1:8159318-8159337 Msa0005490:CDS 40.0%
! TTTGAAGCCTCTTCTTCCAT+GGG - chr1_1:8159337-8159356 None:intergenic 40.0%
!!! TTTTGAAGCCTCTTCTTCCA+TGG - chr1_1:8159338-8159357 None:intergenic 40.0%
CAGGATATTTCAGGATGAGT+TGG + chr1_1:8159378-8159397 Msa0005490:CDS 40.0%
! TTGTCGCATGTTTGAGTTTG+TGG + chr1_1:8159537-8159556 Msa0005490:CDS 40.0%
! TGTCGCATGTTTGAGTTTGT+GGG + chr1_1:8159538-8159557 Msa0005490:CDS 40.0%
CGGCTTAGAACATATTGAGA+AGG - chr1_1:8159619-8159638 None:intergenic 40.0%
! CTCAATATGTTCTAAGCCGA+TGG + chr1_1:8159620-8159639 Msa0005490:CDS 40.0%
AATTGCAATTGAAGACCTCG+AGG + chr1_1:8159675-8159694 Msa0005490:CDS 40.0%
TGCAATTCAAGCTTTACGGA+AGG + chr1_1:8159801-8159820 Msa0005490:CDS 40.0%
CAAGTTTATGGTCTGGTCAT+CGG - chr1_1:8159911-8159930 None:intergenic 40.0%
GATCACACAAGTTTATGGTC+TGG - chr1_1:8159918-8159937 None:intergenic 40.0%
TGGAGGATCACACAAGTTTA+TGG - chr1_1:8159923-8159942 None:intergenic 40.0%
! TTGGACATCTACGTTTGTTG+TGG - chr1_1:8160047-8160066 None:intergenic 40.0%
GTCTGCAACAAACCAATACT+AGG - chr1_1:8160347-8160366 None:intergenic 40.0%
!!! CAGACCTGTTTTTGCCAATA+AGG + chr1_1:8160362-8160381 Msa0005490:intron 40.0%
! AATTTTGCCGAAGTCAAAGG+TGG + chr1_1:8160388-8160407 Msa0005490:three_prime_UTR 40.0%
! AAATCCACCACCTTTGACTT+CGG - chr1_1:8160398-8160417 None:intergenic 40.0%
TCTGATGTGATTCTAGGCTT+TGG + chr1_1:8160419-8160438 Msa0005490:three_prime_UTR 40.0%
! TTTCCCAGCTTTTGTACCTT+TGG + chr1_1:8160515-8160534 Msa0005490:three_prime_UTR 40.0%
CAGATTGTGTTTACTAGGAG+GGG + chr1_1:8160570-8160589 Msa0005490:three_prime_UTR 40.0%
CAGAGCGGTTGTGAAAATGA+AGG - chr1_1:8157225-8157244 None:intergenic 45.0%
GTCGAGAATCAAGTACAGAG+CGG - chr1_1:8157240-8157259 None:intergenic 45.0%
GCAACAGAGAGTTGCAGATT+CGG - chr1_1:8157265-8157284 None:intergenic 45.0%
!! CTTTAGTTTGCTGGTTGTCG+TGG + chr1_1:8157623-8157642 Msa0005490:intron 45.0%
ATCATCTGAGCCATTCATGG+TGG - chr1_1:8157889-8157908 None:intergenic 45.0%
!! TTCCTTCAAAGGGCTCTAAG+GGG - chr1_1:8157968-8157987 None:intergenic 45.0%
CATTCCTTCAAAGGGCTCTA+AGG - chr1_1:8157970-8157989 None:intergenic 45.0%
! CTTAGAGCCCTTTGAAGGAA+TGG + chr1_1:8157968-8157987 Msa0005490:CDS 45.0%
GTACACGATCAAACAAGGGT+AGG + chr1_1:8158179-8158198 Msa0005490:CDS 45.0%
CGAAGATGCTGTGAACTTCT+TGG - chr1_1:8158293-8158312 None:intergenic 45.0%
GTGTTATGTACTGAGAGTGG+TGG + chr1_1:8158398-8158417 Msa0005490:CDS 45.0%
GCATTTCAAATGGACGCAGA+AGG + chr1_1:8158563-8158582 Msa0005490:CDS 45.0%
ATGCCCTTTGTTCCATTCAC+TGG + chr1_1:8158692-8158711 Msa0005490:CDS 45.0%
! AGGAACCCATTTTCCTCGAA+TGG - chr1_1:8159104-8159123 None:intergenic 45.0%
! GTACGTCAGAGTTTTTGTGC+TGG + chr1_1:8159130-8159149 Msa0005490:CDS 45.0%
CATACTTTCACTCCTTTGGG+TGG - chr1_1:8159164-8159183 None:intergenic 45.0%
CCTTTCATTCGTAAAGTGCG+TGG - chr1_1:8159967-8159986 None:intergenic 45.0%
CCACGCACTTTACGAATGAA+AGG + chr1_1:8159964-8159983 Msa0005490:CDS 45.0%
!!! TCTGGCGTGATTTCAGGTTT+TGG + chr1_1:8160449-8160468 Msa0005490:three_prime_UTR 45.0%
!!! TCAGGTTTTGGATGCAGTCT+TGG + chr1_1:8160461-8160480 Msa0005490:three_prime_UTR 45.0%
! GCACCAAAGGTACAAAAGCT+GGG - chr1_1:8160521-8160540 None:intergenic 45.0%
! CAGCTTTTGTACCTTTGGTG+CGG + chr1_1:8160520-8160539 Msa0005490:three_prime_UTR 45.0%
TTACTAGGAGGGGATCTGTT+CGG + chr1_1:8160580-8160599 Msa0005490:three_prime_UTR 45.0%
ACTTTCACTCCTTTGGGTGG+TGG - chr1_1:8159161-8159180 None:intergenic 50.0%
GCAATTGAAGACCTCGAGGA+AGG + chr1_1:8159679-8159698 Msa0005490:CDS 50.0%
GTCTGGTCATCGGAGACTTT+AGG - chr1_1:8159901-8159920 None:intergenic 50.0%
AAGTGCGTGGACGACCTTTA+GGG - chr1_1:8159954-8159973 None:intergenic 50.0%
AAAGTGCGTGGACGACCTTT+AGG - chr1_1:8159955-8159974 None:intergenic 50.0%
TTTGCCGAAGTCAAAGGTGG+TGG + chr1_1:8160391-8160410 Msa0005490:three_prime_UTR 50.0%
!! CTAGGCTTTGGATGCACTTC+TGG + chr1_1:8160431-8160450 Msa0005490:three_prime_UTR 50.0%
TGCACTTCTGGCGTGATTTC+AGG + chr1_1:8160443-8160462 Msa0005490:three_prime_UTR 50.0%
! CGCACCAAAGGTACAAAAGC+TGG - chr1_1:8160522-8160541 None:intergenic 50.0%
! TCTAAGGGGAGCTCTGATGG+CGG - chr1_1:8157954-8157973 None:intergenic 55.0%
CTCCCCTTAGAGCCCTTTGA+AGG + chr1_1:8157963-8157982 Msa0005490:CDS 55.0%
CTGGAATGTCCACCACCCAA+AGG + chr1_1:8159149-8159168 Msa0005490:CDS 55.0%
!! CTCTGTTTCTGCCTTCCTCG+AGG - chr1_1:8159693-8159712 None:intergenic 55.0%
CTTGTGTGATCCTCCACGTG+CGG + chr1_1:8159927-8159946 Msa0005490:CDS 55.0%
!! GGCTCTAAGGGGAGCTCTGA+TGG - chr1_1:8157957-8157976 None:intergenic 60.0%
! GTACCTTTGGTGCGGCTGTG+CGG + chr1_1:8160528-8160547 Msa0005490:three_prime_UTR 60.0%
! TACCTTTGGTGCGGCTGTGC+GGG + chr1_1:8160529-8160548 Msa0005490:three_prime_UTR 60.0%
! CCTTTAGGGACCGCACGTGG+AGG - chr1_1:8159940-8159959 None:intergenic 65.0%
CCTCCACGTGCGGTCCCTAA+AGG + chr1_1:8159937-8159956 Msa0005490:CDS 65.0%
! CGACCTTTAGGGACCGCACG+TGG - chr1_1:8159943-8159962 None:intergenic 65.0%
AACCCGCACAGCCGCACCAA+AGG - chr1_1:8160534-8160553 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 8157201 8160812 8157201 ID=Msa0005490;Name=Msa0005490
chr1_1 mRNA 8157201 8160812 8157201 ID=Msa0005490-mRNA-1;Parent=Msa0005490;Name=Msa0005490-mRNA-1;_AED=0.19;_eAED=0.19;_QI=122|1|1|1|0.33|0|4|451|765
chr1_1 exon 8157201 8157306 8157201 ID=Msa0005490-mRNA-1:exon:2857;Parent=Msa0005490-mRNA-1
chr1_1 exon 8157833 8160082 8157833 ID=Msa0005490-mRNA-1:exon:2858;Parent=Msa0005490-mRNA-1
chr1_1 exon 8160181 8160247 8160181 ID=Msa0005490-mRNA-1:exon:2859;Parent=Msa0005490-mRNA-1
chr1_1 exon 8160365 8160812 8160365 ID=Msa0005490-mRNA-1:exon:2860;Parent=Msa0005490-mRNA-1
chr1_1 five_prime_UTR 8157201 8157306 8157201 ID=Msa0005490-mRNA-1:five_prime_utr;Parent=Msa0005490-mRNA-1
chr1_1 five_prime_UTR 8157833 8157848 8157833 ID=Msa0005490-mRNA-1:five_prime_utr;Parent=Msa0005490-mRNA-1
chr1_1 CDS 8157849 8160082 8157849 ID=Msa0005490-mRNA-1:cds;Parent=Msa0005490-mRNA-1
chr1_1 CDS 8160181 8160244 8160181 ID=Msa0005490-mRNA-1:cds;Parent=Msa0005490-mRNA-1
chr1_1 three_prime_UTR 8160245 8160247 8160245 ID=Msa0005490-mRNA-1:three_prime_utr;Parent=Msa0005490-mRNA-1
chr1_1 three_prime_UTR 8160365 8160812 8160365 ID=Msa0005490-mRNA-1:three_prime_utr;Parent=Msa0005490-mRNA-1
Gene Sequence

>Msa0005490

ATGACTGATCAAGAAATGACAGATATGCATGAACATACCACCATGAATGGCTCAGATGATGTACTTGCAAATGCTATTGATGCAGATTCTCTCAATGATAGACCGCCATCAGAGCTCCCCTTAGAGCCCTTTGAAGGAATGGAGTTTGCATCAATTGAAGATGTAAAGGATTACTACGTAAGGTACGCCAAGAAAAAAGGTTTTAGTTTTCGCATGGGTCGTGTTACTAAATCAAGAACGAATGGTGTTGTGATTGGTCAAGAGATTCTTTGTTCAAAGGAGGGTTTTAGATCCAAAAAAAAATTGAAAAAAGAAAATGGTAGTCTTAAAGTACACGATCAAACAAGGGTAGGATGCAAGTCAATGCTGTACATCAAGAAAAATGAAGATGGGTGGATTGTTTCCCGATTTGTAAGAGACCATAATCATGTACTATTTTCCCCAAGAAGTTCACAGCATCTTCGAGTACATAGAAAGAAAACCTCAGTGCAAAAAAAACTTATTGATGTGCTTCATGATTCTGGTATAGGCCCTAGTAAAATAGCATCAGTGTTATGTACTGAGAGTGGTGGTGTTGATAATGTTGGATTTAGCCAACAAGATGTTATTAATTATTTAAGTGTAAAGAGACAAAAACAATTAGAAAATGGAGATGCTCACTTGATGTTATCGTACTTTAAGAATTGTCAGTTGAAGAATCCTGGGTTTTTTTATGCATTTCAAATGGACGCAGAAGGTAAATTAGCAAATTGCTTTTGGGTTGATTCAAGGTCTAGAATGGCTTACAAATATTTTGGTGATGTGGTGACTTTTGATCCAACATACTTAACAAATAAATACCTAATGCCCTTTGTTCCATTCACTGGAGTTAATCATCACCAACAATCTATACTTTTTGGTTGTGCTCTGTTATGGGATGAAACTGAAGAGAGTTTTGTTTGGTTGCTACGTACTTGGTTAGAAGCAATGGATGGAGTTAGTCCTAAAACTATTATCACTGATCAAGACACTTCTATTTCTAATGCAGTTGCAAAGGTTTTTCCAGAGGTGAATCATCATTATTGTATGTGGCATATTGAGAAGAAAGTTCCAGAGTACTTGAACCAAATCTATCATGAACATAGCGAGTTTAAAAATCAATTTCACAAGTGCATTCACCAAACTATCACTTCTGATGACTTTGATTCTGATTGGGGAGCAATGATAGATAAGTACGAGTTGCAAGATAACAAATGGCTAAAGAAGATCTACACCATTCGAGGAAAATGGGTTCCTTCTTATGTACGTCAGAGTTTTTGTGCTGGAATGTCCACCACCCAAAGGAGTGAAAGTATGAATAAATTTTTTAAGGGATTTTTGAATTCAAGTACTCCATTGAGCAAGTTTGTGATACAGTATGAGAAGGCTCTTGACGCACGTTATAACAAGGAAAGAGAGAAGAATGTCAAGACAAGGAATTCAAAACCACTTCTGCGTACTTTATATCCCATGGAAGAAGAGGCTTCAAAAATTTATACAAGAAAAGTGTTCAGGATATTTCAGGATGAGTTGGTTGGGTCCCAAATATTTATTGCAGAGAAAGTTAAGTTTTTTGATGAAGTTTCCACATACAAAATTCATGAAATTTACAAAGAAAAACCTAACTATCATGTGACTTTTCAGATCATTTCAAAAGAAGCAAAATGTAGTTGTCGCATGTTTGAGTTTGTGGGAATTCTCTGTAGACATGTATTAAACGTATTCATAAAGGAAAATGTCTATTCTCTCCCTTCTCAATATGTTCTAAGCCGATGGACCATAAATGCTAAGAAAGAAAAAAGTAAAGGAATTGCAATTGAAGACCTCGAGGAAGGCAGAAACAGAGCTTCAAGTACTTCATTATTTAATAGTATTATGGTTGAGTCTCTTGAACTATCTGAAAGAGGTTCACGATCAGAAAAGCATCATGATATTGCAATTCAAGCTTTACGGAAGGCAATCACAAAACTTGATCAATTGGATCTTGAAGAACCAAATGAAGAGTTTGATAAATCGACATTTGAAGTTATGCCTAAAGTCTCCGATGACCAGACCATAAACTTGTGTGATCCTCCACGTGCGGTCCCTAAAGGTCGTCCACGCACTTTACGAATGAAAGGCAGTTTGGAATTGCACAAGAAAAATTCAACCAGTTGTAGTTATTGCAATGGAAAAGGCCACAACAAACGTAGATGTCCAAGTTTAAAACATGGAAGGTGTGATATCATCAATGGTCAACTTTCAAATTCCAATGTTAAAGCTTCTAGTTTGGTTGATTAA
Protein sequence

>Msa0005490

MTDQEMTDMHEHTTMNGSDDVLANAIDADSLNDRPPSELPLEPFEGMEFASIEDVKDYYVRYAKKKGFSFRMGRVTKSRTNGVVIGQEILCSKEGFRSKKKLKKENGSLKVHDQTRVGCKSMLYIKKNEDGWIVSRFVRDHNHVLFSPRSSQHLRVHRKKTSVQKKLIDVLHDSGIGPSKIASVLCTESGGVDNVGFSQQDVINYLSVKRQKQLENGDAHLMLSYFKNCQLKNPGFFYAFQMDAEGKLANCFWVDSRSRMAYKYFGDVVTFDPTYLTNKYLMPFVPFTGVNHHQQSILFGCALLWDETEESFVWLLRTWLEAMDGVSPKTIITDQDTSISNAVAKVFPEVNHHYCMWHIEKKVPEYLNQIYHEHSEFKNQFHKCIHQTITSDDFDSDWGAMIDKYELQDNKWLKKIYTIRGKWVPSYVRQSFCAGMSTTQRSESMNKFFKGFLNSSTPLSKFVIQYEKALDARYNKEREKNVKTRNSKPLLRTLYPMEEEASKIYTRKVFRIFQDELVGSQIFIAEKVKFFDEVSTYKIHEIYKEKPNYHVTFQIISKEAKCSCRMFEFVGILCRHVLNVFIKENVYSLPSQYVLSRWTINAKKEKSKGIAIEDLEEGRNRASSTSLFNSIMVESLELSERGSRSEKHHDIAIQALRKAITKLDQLDLEEPNEEFDKSTFEVMPKVSDDQTINLCDPPRAVPKGRPRTLRMKGSLELHKKNSTSCSYCNGKGHNKRRCPSLKHGRCDIINGQLSNSNVKASSLVD*