AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0324640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0324640 MtrunA17_Chr1g0202341 74.852 676 168 1 4 679 12 685 0.0 1075
Msa0324640 MtrunA17_Chr5g0448761 43.701 643 342 6 36 666 53 687 0.0 560
Msa0324640 MtrunA17_Chr5g0440451 35.148 643 379 9 42 666 42 664 1.61e-132 416
Msa0324640 MtrunA17_Chr1g0207551 35.910 621 385 5 41 660 32 640 1.04e-131 410
Msa0324640 MtrunA17_Chr5g0441581 35.964 659 385 13 20 664 2 637 7.08e-131 404
Msa0324640 MtrunA17_Chr5g0448511 40.733 464 267 3 206 666 1 459 5.48e-129 395
Msa0324640 MtrunA17_Chr2g0296781 34.207 687 393 13 47 710 1 651 2.90e-125 391
Msa0324640 MtrunA17_Chr3g0080351 34.123 633 370 7 41 659 74 673 1.27e-121 385
Msa0324640 MtrunA17_Chr3g0118611 38.023 526 302 6 218 724 118 638 1.15e-119 378
Msa0324640 MtrunA17_Chr2g0329031 33.439 628 395 10 42 667 13 619 2.41e-119 376
Msa0324640 MtrunA17_Chr2g0287061 35.601 632 343 11 41 663 181 757 1.49e-117 373
Msa0324640 MtrunA17_Chr2g0287061 27.586 174 93 5 17 178 9 161 2.88e-15 80.1
Msa0324640 MtrunA17_Chr2g0324011 34.091 572 365 6 37 603 4 568 2.77e-114 358
Msa0324640 MtrunA17_Chr2g0291581 32.927 656 399 12 23 663 50 679 6.67e-113 357
Msa0324640 MtrunA17_Chr8g0363841 31.970 660 412 11 31 664 3 651 7.89e-113 357
Msa0324640 MtrunA17_Chr6g0477261 36.101 554 331 9 165 710 2 540 1.03e-111 353
Msa0324640 MtrunA17_Chr2g0287651 30.342 702 443 10 47 738 79 744 1.90e-105 343
Msa0324640 MtrunA17_Chr1g0198761 30.350 715 457 12 44 742 48 737 4.57e-105 339
Msa0324640 MtrunA17_Chr1g0203721 31.040 625 422 6 46 666 66 685 3.00e-104 340
Msa0324640 MtrunA17_Chr1g0196541 34.397 564 345 9 47 598 1 551 6.90e-104 335
Msa0324640 MtrunA17_Chr6g0458391 32.920 565 351 6 43 603 225 765 5.00e-102 331
Msa0324640 MtrunA17_Chr6g0458391 27.749 191 126 4 23 204 71 258 6.06e-13 72.4
Msa0324640 MtrunA17_Chr3g0082121 31.738 627 408 9 22 645 22 631 5.04e-99 321
Msa0324640 MtrunA17_Chr2g0291591 33.661 508 327 6 42 547 63 562 2.92e-94 305
Msa0324640 MtrunA17_Chr1g0203051 29.642 587 399 6 11 591 44 622 3.43e-90 296
Msa0324640 MtrunA17_Chr2g0276841 28.526 631 422 12 43 663 70 681 1.64e-80 272
Msa0324640 MtrunA17_Chr2g0294841 26.685 727 480 15 41 738 43 745 2.18e-80 273
Msa0324640 MtrunA17_Chr2g0290391 29.148 669 434 16 12 663 21 666 3.08e-76 260
Msa0324640 MtrunA17_Chr1g0164591 36.842 304 183 2 134 437 14 308 4.29e-67 225
Msa0324640 MtrunA17_Chr5g0431001 31.847 471 299 10 5 456 16 483 2.60e-65 226
Msa0324640 MtrunA17_Chr7g0258861 36.770 291 179 4 35 322 2 290 8.25e-60 204
Msa0324640 MtrunA17_Chr3g0077241 76.087 92 18 2 6 96 1 89 2.00e-39 141
Msa0324640 MtrunA17_Chr4g0052331 53.684 95 44 0 1 95 1 95 1.01e-27 107
Msa0324640 MtrunA17_Chr2g0308841 28.054 221 152 3 383 599 6 223 1.70e-24 103
Msa0324640 MtrunA17_Chr2g0329361 35.099 151 86 3 5 148 22 167 4.53e-23 99.4
Msa0324640 MtrunA17_Chr2g0329421 30.921 152 82 3 42 173 26 174 7.55e-20 89.0
Msa0324640 MtrunA17_Chr1g0149131 32.727 110 74 0 323 432 1 110 8.72e-20 85.9
Msa0324640 MtrunA17_Chr4g0033611 29.560 159 88 3 36 173 69 224 1.42e-18 86.3
Msa0324640 MtrunA17_Chr3g0078041 36.842 114 67 4 42 152 49 160 3.62e-16 78.6
Msa0324640 MtrunA17_Chr7g0233911 36.449 107 62 3 42 145 33 136 3.48e-15 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0324640 AT4G38180.1 41.568 676 370 9 2 659 21 689 0.0 539
Msa0324640 AT4G15090.1 34.816 652 408 8 37 681 45 686 2.58e-136 423
Msa0324640 AT4G15090.5 34.816 652 408 8 37 681 45 686 2.58e-136 423
Msa0324640 AT4G15090.3 34.816 652 408 8 37 681 45 686 2.58e-136 423
Msa0324640 AT4G15090.2 34.816 652 408 8 37 681 45 686 2.58e-136 423
Msa0324640 AT4G15090.4 34.816 652 408 8 37 681 45 686 2.58e-136 423
Msa0324640 AT2G27110.2 36.000 625 360 9 42 663 48 635 2.61e-128 403
Msa0324640 AT2G27110.1 36.000 625 360 9 42 663 48 635 2.61e-128 403
Msa0324640 AT3G06250.2 34.913 633 340 11 42 663 189 760 2.93e-126 395
Msa0324640 AT3G06250.2 33.962 106 54 2 41 145 26 116 1.89e-14 77.8
Msa0324640 AT3G06250.1 34.913 633 340 11 42 663 189 760 2.93e-126 395
Msa0324640 AT3G06250.1 33.962 106 54 2 41 145 26 116 1.89e-14 77.8
Msa0324640 AT3G06250.3 34.913 633 340 11 42 663 189 760 2.93e-126 395
Msa0324640 AT3G06250.3 33.962 106 54 2 41 145 26 116 1.89e-14 77.8
Msa0324640 AT5G18960.2 35.118 635 339 11 42 663 210 784 6.34e-125 392
Msa0324640 AT5G18960.2 31.132 106 57 2 41 145 41 131 3.40e-13 73.9
Msa0324640 AT5G18960.1 35.118 635 339 11 42 663 210 784 6.34e-125 392
Msa0324640 AT5G18960.1 31.132 106 57 2 41 145 41 131 3.40e-13 73.9
Msa0324640 AT2G27110.3 40.135 446 261 2 218 663 51 490 2.43e-117 370
Msa0324640 AT3G22170.2 31.024 635 393 7 39 660 66 668 2.10e-112 361
Msa0324640 AT3G22170.1 31.024 635 393 7 39 660 66 668 2.10e-112 361
Msa0324640 AT1G76320.3 32.039 618 397 8 47 662 1 597 2.61e-109 348
Msa0324640 AT1G76320.2 32.039 618 397 8 47 662 1 597 1.09e-108 348
Msa0324640 AT1G76320.1 32.039 618 397 8 47 662 1 597 1.38e-108 348
Msa0324640 AT1G76320.4 32.039 618 397 8 47 662 1 597 1.38e-108 348
Msa0324640 AT4G38170.1 36.250 480 292 6 222 698 21 489 2.56e-106 336
Msa0324640 AT1G52520.1 29.542 677 419 12 11 667 61 699 1.30e-101 328
Msa0324640 AT2G32250.5 31.200 625 373 7 46 667 41 611 5.06e-99 323
Msa0324640 AT2G32250.6 31.200 625 373 7 46 667 41 611 1.03e-98 323
Msa0324640 AT2G32250.1 31.200 625 373 7 46 667 41 611 1.03e-98 323
Msa0324640 AT2G32250.3 31.200 625 373 7 46 667 41 611 1.03e-98 323
Msa0324640 AT2G32250.2 31.200 625 373 7 46 667 41 611 1.12e-98 323
Msa0324640 AT2G32250.4 31.200 625 373 7 46 667 41 611 1.12e-98 323
Msa0324640 AT2G32250.8 31.090 624 373 7 47 667 1 570 5.25e-98 321
Msa0324640 AT2G32250.7 31.090 624 373 7 47 667 1 570 7.98e-98 320
Msa0324640 AT4G19990.3 30.920 663 394 12 41 684 19 636 9.30e-98 320
Msa0324640 AT4G19990.4 30.920 663 394 12 41 684 19 636 9.30e-98 320
Msa0324640 AT4G19990.5 30.920 663 394 12 41 684 19 636 1.41e-97 319
Msa0324640 AT4G19990.6 30.920 663 394 12 41 684 19 636 1.45e-97 320
Msa0324640 AT4G19990.2 30.920 663 394 12 41 684 19 636 1.45e-97 320
Msa0324640 AT4G19990.7 30.920 663 394 12 41 684 33 650 2.04e-97 319
Msa0324640 AT4G19990.1 31.652 575 346 9 115 684 47 579 2.64e-91 301
Msa0324640 AT1G80010.1 28.863 686 430 21 12 672 73 725 6.44e-89 295
Msa0324640 AT5G28530.1 28.774 636 416 14 43 657 55 674 1.94e-78 266
Msa0324640 AT5G28530.3 28.459 636 414 14 43 657 55 670 9.29e-77 261
Msa0324640 AT5G28530.2 28.459 636 414 14 43 657 55 670 9.29e-77 261
Msa0324640 AT1G10240.2 28.855 655 425 17 27 658 25 661 4.57e-76 259
Msa0324640 AT1G10240.1 28.855 655 425 17 27 658 25 661 4.57e-76 259
Msa0324640 AT5G28530.4 25.237 634 378 18 43 657 55 611 6.48e-50 186
Msa0324640 AT5G28530.5 24.921 634 376 18 43 657 55 607 2.29e-48 181
Msa0324640 AT3G59470.3 39.450 109 62 2 42 149 69 174 2.56e-20 91.7
Msa0324640 AT3G59470.1 39.450 109 62 2 42 149 69 174 2.56e-20 91.7
Msa0324640 AT3G59470.2 39.450 109 62 2 42 149 81 186 3.73e-20 91.7
Msa0324640 AT3G07500.1 38.318 107 63 2 41 146 31 135 9.46e-16 77.4
Msa0324640 AT2G43280.1 31.538 130 84 4 27 153 6 133 3.72e-15 75.5
Msa0324640 AT4G12850.2 34.286 105 63 2 42 145 10 109 5.62e-15 73.2
Msa0324640 AT4G12850.4 31.746 126 78 3 42 164 10 130 6.88e-15 72.8
Msa0324640 AT4G12850.1 35.238 105 62 2 42 145 10 109 9.97e-15 73.6
Msa0324640 AT2G43280.2 32.323 99 62 4 58 153 2 98 6.87e-11 62.0

Find 153 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTCTGCAATAAATATT+TGG 0.060470 2_4:-30487699 None:intergenic
TGCACTTCTGGCGTGATTTC+AGG 0.168073 2_4:+30488734 Msa0324640:three_prime_UTR
AGAATGGCTTACAAATATTT+TGG 0.184037 2_4:+30486914 Msa0324640:CDS
TCGGCAAAATTATCCCTTAT+TGG 0.193753 2_4:-30488667 None:intergenic
TTATACAACTTTACTATAAA+TGG 0.200880 2_4:-30489094 None:intergenic
GATTTCTCTGATGTGATTCT+AGG 0.204971 2_4:+30488704 Msa0324640:three_prime_UTR
TCTACACCATTCGAGGAAAA+TGG 0.218597 2_4:+30487386 Msa0324640:CDS
TTCATTATTTAATAGTATTA+TGG 0.222727 2_4:+30488014 Msa0324640:CDS
CTAGGCTTTGGATGCACTTC+TGG 0.230580 2_4:+30488722 Msa0324640:three_prime_UTR
GTAAATTAGCAAATTGCTTT+TGG 0.235893 2_4:+30486876 Msa0324640:CDS
AAAAGTGTTCAGGATATTTC+AGG 0.242179 2_4:+30487660 Msa0324640:CDS
ATTGATGTGCTTCATGATTC+TGG 0.246659 2_4:+30486641 Msa0324640:CDS
CATTCCTTCAAAGGGCTCTA+AGG 0.261818 2_4:-30486258 None:intergenic
TATTCATACTTTCACTCCTT+TGG 0.266644 2_4:-30487456 None:intergenic
CGCTATGTTCATGATAGATT+TGG 0.274190 2_4:-30487243 None:intergenic
ATTCATACTTTCACTCCTTT+GGG 0.296462 2_4:-30487455 None:intergenic
CTGATGACTTTGATTCTGAT+TGG 0.300769 2_4:+30487311 Msa0324640:CDS
TCTGGCGTGATTTCAGGTTT+TGG 0.301040 2_4:+30488740 Msa0324640:three_prime_UTR
AAAGTGCGTGGACGACCTTT+AGG 0.302776 2_4:-30488243 None:intergenic
TGATGACTTTGATTCTGATT+GGG 0.310601 2_4:+30487312 Msa0324640:CDS
ATATTGCAATTCAAGCTTTA+CGG 0.318900 2_4:+30488088 Msa0324640:CDS
ATCCTTCCATTTCTTATTGA+AGG 0.322059 2_4:+30485576 Msa0324640:five_prime_UTR
CGAAGATGCTGTGAACTTCT+TGG 0.326106 2_4:-30486581 None:intergenic
TTTCTCTGCAATAAATATTT+GGG 0.327509 2_4:-30487698 None:intergenic
TCTGATGTGATTCTAGGCTT+TGG 0.327651 2_4:+30488710 Msa0324640:three_prime_UTR
AATAATTAATAACATCTTGT+TGG 0.331163 2_4:-30486733 None:intergenic
TGAAAAGTCACATGATAGTT+AGG 0.341747 2_4:-30487778 None:intergenic
GAAGATGCTGTGAACTTCTT+GGG 0.347598 2_4:-30486580 None:intergenic
TCCATTGCAATAACTACAAC+TGG 0.351484 2_4:-30488307 None:intergenic
TGGAGGATCACACAAGTTTA+TGG 0.352054 2_4:-30488211 None:intergenic
AAGTGCGTGGACGACCTTTA+GGG 0.355019 2_4:-30488242 None:intergenic
ATGCCCTTTGTTCCATTCAC+TGG 0.364662 2_4:+30486983 Msa0324640:CDS
CAATGTTAAAGCTTCTAGTT+TGG 0.368597 2_4:+30488505 Msa0324640:CDS
TATTTATTTGTTAAGTATGT+TGG 0.372145 2_4:-30486956 None:intergenic
TTTACGAATGAAAGGCAGTT+TGG 0.374955 2_4:+30488263 Msa0324640:CDS
TTCTAGTTTGGTTGATTAAG+AGG 0.375412 2_4:+30488517 Msa0324640:exon
TTTATACAAGAAAAGTGTTC+AGG 0.375460 2_4:+30487650 Msa0324640:CDS
GATTATGGTCTCTTACAAAT+CGG 0.380412 2_4:-30486544 None:intergenic
GATTTATCAAACTCTTCATT+TGG 0.382353 2_4:-30488150 None:intergenic
TTGTCGCATGTTTGAGTTTG+TGG 0.384532 2_4:+30487828 Msa0324640:CDS
AATTGTCAGTTGAAGAATCC+TGG 0.396913 2_4:+30486821 Msa0324640:CDS
TCCATACCTTCAATAAGAAA+TGG 0.399424 2_4:-30485582 None:intergenic
TGTATTAAACGTATTCATAA+AGG 0.409882 2_4:+30487867 Msa0324640:CDS
GGGAAAATAGTACATGATTA+TGG 0.411615 2_4:-30486559 None:intergenic
AATCACAAAACTTGATCAAT+TGG 0.412422 2_4:+30488116 Msa0324640:CDS
TACCTTTGGTGCGGCTGTGC+GGG 0.417771 2_4:+30488820 Msa0324640:three_prime_UTR
TGAATGGAACAAAGGGCATT+AGG 0.418217 2_4:-30486979 None:intergenic
CGCACCAAAGGTACAAAAGC+TGG 0.418500 2_4:-30488810 None:intergenic
TTGTGAAAATGAAGGAGAAC+AGG 0.419556 2_4:-30485505 None:intergenic
TTGTTTGGTTGCTACGTACT+TGG 0.423904 2_4:+30487074 Msa0324640:CDS
AGTCATCAGAAGTGATAGTT+TGG 0.427220 2_4:-30487297 None:intergenic
GCAACAGAGAGTTGCAGATT+CGG 0.435446 2_4:-30485553 None:intergenic
TGCATCAATTGAAGATGTAA+AGG 0.436857 2_4:+30486286 Msa0324640:CDS
GTGCTTCATGATTCTGGTAT+AGG 0.438869 2_4:+30486647 Msa0324640:CDS
ACTTTCACTCCTTTGGGTGG+TGG 0.440297 2_4:-30487449 None:intergenic
TGTAGTTATTGCAATGGAAA+AGG 0.442086 2_4:+30488312 Msa0324640:CDS
GGCTCTAAGGGGAGCTCTGA+TGG 0.445219 2_4:-30486245 None:intergenic
TACATCATCTGAGCCATTCA+TGG 0.452459 2_4:-30486180 None:intergenic
CATCTACATAACAGGATTGT+GGG 0.453044 2_4:+30486110 Msa0324640:intron
TTTAGGTGTGATATCATCAA+TGG 0.453788 2_4:+30488467 Msa0324640:intron
CATGAACATACCACCATGAA+TGG 0.455856 2_4:+30486167 Msa0324640:CDS
CTCCCCTTAGAGCCCTTTGA+AGG 0.456460 2_4:+30486254 Msa0324640:CDS
GGTTGATTCAAGGTCTAGAA+TGG 0.460592 2_4:+30486898 Msa0324640:CDS
TATTTCAGGATGAGTTGGTT+GGG 0.463690 2_4:+30487674 Msa0324640:CDS
AAGAAGATCTACACCATTCG+AGG 0.468875 2_4:+30487379 Msa0324640:CDS
CTACACCATTCGAGGAAAAT+GGG 0.470377 2_4:+30487387 Msa0324640:CDS
AGTGGTGGTGTTGATAATGT+TGG 0.474266 2_4:+30486704 Msa0324640:CDS
ATTATGGTCTCTTACAAATC+GGG 0.474650 2_4:-30486543 None:intergenic
CTTAGAGCCCTTTGAAGGAA+TGG 0.476103 2_4:+30486259 Msa0324640:CDS
GATGACTTTGATTCTGATTG+GGG 0.477884 2_4:+30487313 Msa0324640:CDS
ACCAGTTGTAGTTATTGCAA+TGG 0.478462 2_4:+30488306 Msa0324640:CDS
CAGGATATTTCAGGATGAGT+TGG 0.484717 2_4:+30487669 Msa0324640:CDS
TGCAAACTCCATTCCTTCAA+AGG 0.485592 2_4:-30486267 None:intergenic
TGTCGCATGTTTGAGTTTGT+GGG 0.487042 2_4:+30487829 Msa0324640:CDS
CAAACTAGAAGCTTTAACAT+TGG 0.487651 2_4:-30488504 None:intergenic
AAATCCACCACCTTTGACTT+CGG 0.488240 2_4:-30488686 None:intergenic
ATTCCTTCAAAGGGCTCTAA+GGG 0.488264 2_4:-30486257 None:intergenic
CTCAATATGTTCTAAGCCGA+TGG 0.491066 2_4:+30487911 Msa0324640:CDS
TCTTAGCATTTATGGTCCAT+CGG 0.492991 2_4:-30487927 None:intergenic
TTAAAGTAAAATGTTAAAAG+AGG 0.495012 2_4:-30488985 None:intergenic
ACGAGTTGCAAGATAACAAA+TGG 0.498356 2_4:+30487353 Msa0324640:CDS
ATTGTCAGTTGAAGAATCCT+GGG 0.500058 2_4:+30486822 Msa0324640:CDS
TAGATTTGGTTCAAGTACTC+TGG 0.502715 2_4:-30487229 None:intergenic
TTACTAGGAGGGGATCTGTT+CGG 0.504637 2_4:+30488871 Msa0324640:three_prime_UTR
TTTGAAGCCTCTTCTTCCAT+GGG 0.505212 2_4:-30487625 None:intergenic
TCAAGAGATTCTTTGTTCAA+AGG 0.507548 2_4:+30486397 Msa0324640:CDS
GCAAACTCCATTCCTTCAAA+GGG 0.507732 2_4:-30486266 None:intergenic
TTTGCCGAAGTCAAAGGTGG+TGG 0.512830 2_4:+30488682 Msa0324640:three_prime_UTR
CAATAATGATGATTCACCTC+TGG 0.513933 2_4:-30487181 None:intergenic
TCAGATTGTGTTTACTAGGA+GGG 0.514506 2_4:+30488860 Msa0324640:three_prime_UTR
AAACTCTGACGTACATAAGA+AGG 0.521777 2_4:-30487412 None:intergenic
AGAACGAATGGTGTTGTGAT+TGG 0.522138 2_4:+30486374 Msa0324640:CDS
CCTCCACGTGCGGTCCCTAA+AGG 0.522617 2_4:+30488228 Msa0324640:CDS
TGTATCACAAACTTGCTCAA+TGG 0.525322 2_4:-30487511 None:intergenic
GTCTGGTCATCGGAGACTTT+AGG 0.525728 2_4:-30488189 None:intergenic
CATACTTTCACTCCTTTGGG+TGG 0.526942 2_4:-30487452 None:intergenic
TCTGCGTACTTTATATCCCA+TGG 0.529545 2_4:+30487609 Msa0324640:CDS
ACTCCAGTGAATGGAACAAA+GGG 0.535906 2_4:-30486986 None:intergenic
ACGTACTTGGTTAGAAGCAA+TGG 0.537041 2_4:+30487087 Msa0324640:CDS
CAGATTGTGTTTACTAGGAG+GGG 0.539634 2_4:+30488861 Msa0324640:three_prime_UTR
TCTCTTGAACTATCTGAAAG+AGG 0.540310 2_4:+30488042 Msa0324640:CDS
AGAGATTCTTTGTTCAAAGG+AGG 0.541828 2_4:+30486400 Msa0324640:CDS
TTTATATCCCATGGAAGAAG+AGG 0.541904 2_4:+30487618 Msa0324640:CDS
CAAGTTTATGGTCTGGTCAT+CGG 0.542082 2_4:-30488199 None:intergenic
TGAATCATCATTATTGTATG+TGG 0.542391 2_4:+30487188 Msa0324640:CDS
TTGGACATCTACGTTTGTTG+TGG 0.543214 2_4:-30488335 None:intergenic
TGCAATTCAAGCTTTACGGA+AGG 0.544992 2_4:+30488092 Msa0324640:CDS
ATATTTCAGGATGAGTTGGT+TGG 0.547184 2_4:+30487673 Msa0324640:CDS
GTACCTTTGGTGCGGCTGTG+CGG 0.547232 2_4:+30488819 Msa0324640:three_prime_UTR
ACATCTACATAACAGGATTG+TGG 0.547572 2_4:+30486109 Msa0324640:intron
GAGATTCTTTGTTCAAAGGA+GGG 0.548544 2_4:+30486401 Msa0324640:CDS
TATTTCTAATGCAGTTGCAA+AGG 0.550472 2_4:+30487153 Msa0324640:CDS
GATCACACAAGTTTATGGTC+TGG 0.557810 2_4:-30488206 None:intergenic
TTCAGATTGTGTTTACTAGG+AGG 0.562996 2_4:+30488859 Msa0324640:three_prime_UTR
GCACCAAAGGTACAAAAGCT+GGG 0.568721 2_4:-30488809 None:intergenic
CGGCTTAGAACATATTGAGA+AGG 0.582615 2_4:-30487907 None:intergenic
GTCCAAGTTTAAAACATGGA+AGG 0.582831 2_4:+30488352 Msa0324640:CDS
AACTCCAGTGAATGGAACAA+AGG 0.585483 2_4:-30486987 None:intergenic
TGATGATTAACTCCAGTGAA+TGG 0.587906 2_4:-30486995 None:intergenic
TTAAAGTACACGATCAAACA+AGG 0.591318 2_4:+30486465 Msa0324640:CDS
GTTACTAAATCAAGAACGAA+TGG 0.591678 2_4:+30486362 Msa0324640:CDS
AAGATGCTGTGAACTTCTTG+GGG 0.593260 2_4:-30486579 None:intergenic
ATGTAAAGGATTACTACGTA+AGG 0.595028 2_4:+30486300 Msa0324640:CDS
GGCTTAGAACATATTGAGAA+GGG 0.595706 2_4:-30487906 None:intergenic
TACCTTCAATAAGAAATGGA+AGG 0.597326 2_4:-30485578 None:intergenic
GCATTTCAAATGGACGCAGA+AGG 0.597955 2_4:+30486854 Msa0324640:CDS
CCACGCACTTTACGAATGAA+AGG 0.600807 2_4:+30488255 Msa0324640:CDS
TCTAAGGGGAGCTCTGATGG+CGG 0.601475 2_4:-30486242 None:intergenic
GCAATTGAAGACCTCGAGGA+AGG 0.603885 2_4:+30487970 Msa0324640:CDS
CCTTTCATTCGTAAAGTGCG+TGG 0.604290 2_4:-30488255 None:intergenic
GGATATAAAGTACGCAGAAG+TGG 0.606925 2_4:-30487604 None:intergenic
CTCTGTTTCTGCCTTCCTCG+AGG 0.612225 2_4:-30487981 None:intergenic
AACCCGCACAGCCGCACCAA+AGG 0.615562 2_4:-30488822 None:intergenic
CAGAGCGGTTGTGAAAATGA+AGG 0.616006 2_4:-30485513 None:intergenic
GAGAGAAGAATGTCAAGACA+AGG 0.616684 2_4:+30487572 Msa0324640:CDS
GAACAGGGTAACTGATGTTG+AGG 0.619611 2_4:-30485489 None:intergenic
CGACCTTTAGGGACCGCACG+TGG 0.624526 2_4:-30488231 None:intergenic
GTTTGTGATACAGTATGAGA+AGG 0.624921 2_4:+30487522 Msa0324640:CDS
GTACACGATCAAACAAGGGT+AGG 0.637469 2_4:+30486470 Msa0324640:CDS
CTGGAATGTCCACCACCCAA+AGG 0.639739 2_4:+30487440 Msa0324640:CDS
AGATGTCCAAGTTTAAAACA+TGG 0.643966 2_4:+30488348 Msa0324640:CDS
ACTTGGTTAGAAGCAATGGA+TGG 0.652582 2_4:+30487091 Msa0324640:CDS
TCTTGACGCACGTTATAACA+AGG 0.672022 2_4:+30487546 Msa0324640:CDS
TTCCTTCAAAGGGCTCTAAG+GGG 0.687755 2_4:-30486256 None:intergenic
TGTGAAAATGAAGGAGAACA+GGG 0.696875 2_4:-30485504 None:intergenic
CCTTTAGGGACCGCACGTGG+AGG 0.703909 2_4:-30488228 None:intergenic
ATCATCTGAGCCATTCATGG+TGG 0.705037 2_4:-30486177 None:intergenic
AATTGCAATTGAAGACCTCG+AGG 0.709068 2_4:+30487966 Msa0324640:CDS
CTTGTGTGATCCTCCACGTG+CGG 0.710705 2_4:+30488218 Msa0324640:CDS
TAAAGTACACGATCAAACAA+GGG 0.712268 2_4:+30486466 Msa0324640:CDS
GTGTTATGTACTGAGAGTGG+TGG 0.725945 2_4:+30486689 Msa0324640:CDS
TCAGTGTTATGTACTGAGAG+TGG 0.733515 2_4:+30486686 Msa0324640:CDS
GTCGAGAATCAAGTACAGAG+CGG 0.751791 2_4:-30485528 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATATAAGTAAAAAAG+TGG - chr2_4:30486036-30486055 None:intergenic 10.0%
!!! TTCTTTTTTCAATTTTTTTT+TGG - chr2_4:30486435-30486454 None:intergenic 10.0%
!! AAAAAATTGAAAAAAGAAAA+TGG + chr2_4:30486437-30486456 Msa0324640:CDS 10.0%
!!! AAGTATGAATAAATTTTTTA+AGG + chr2_4:30487468-30487487 Msa0324640:CDS 10.0%
!!! AGTATGAATAAATTTTTTAA+GGG + chr2_4:30487469-30487488 Msa0324640:CDS 10.0%
!! TTCATTATTTAATAGTATTA+TGG + chr2_4:30488014-30488033 Msa0324640:CDS 10.0%
!!! TAACATTTTACTTTAATATA+AGG + chr2_4:30488992-30489011 Msa0324640:three_prime_UTR 10.0%
!! ACAATAATATGCTATTATAA+AGG - chr2_4:30485982-30486001 None:intergenic 15.0%
!! CATATATATAAGTAAAAAAG+TGG - chr2_4:30486068-30486087 None:intergenic 15.0%
!! AATAATTAATAACATCTTGT+TGG - chr2_4:30486736-30486755 None:intergenic 15.0%
!!! TATTTATTTGTTAAGTATGT+TGG - chr2_4:30486959-30486978 None:intergenic 15.0%
!! CTTGTAACAATAATATATTT+AGG - chr2_4:30488592-30488611 None:intergenic 15.0%
!!! CTTTTATTGTTTTTCTTTTT+AGG + chr2_4:30488949-30488968 Msa0324640:three_prime_UTR 15.0%
!! TTAAAGTAAAATGTTAAAAG+AGG - chr2_4:30488988-30489007 None:intergenic 15.0%
!!! TATTCACAATCTTTGTTTTT+TGG + chr2_4:30485624-30485643 Msa0324640:intron 20.0%
!! TGAAAAAACCATAATAATAG+AGG - chr2_4:30485832-30485851 None:intergenic 20.0%
!!! AAAACTAAAACCTTTTTTCT+TGG - chr2_4:30486330-30486349 None:intergenic 20.0%
!! AGACAAAAACAATTAGAAAA+TGG + chr2_4:30486767-30486786 Msa0324640:CDS 20.0%
!!! GTTTTTTTATGCATTTCAAA+TGG + chr2_4:30486844-30486863 Msa0324640:CDS 20.0%
!!! TAAATTAGCAAATTGCTTTT+GGG + chr2_4:30486877-30486896 Msa0324640:CDS 20.0%
!! TTTCTCTGCAATAAATATTT+GGG - chr2_4:30487701-30487720 None:intergenic 20.0%
!!! AATTTCATGAATTTTGTATG+TGG - chr2_4:30487746-30487765 None:intergenic 20.0%
!! TGTATTAAACGTATTCATAA+AGG + chr2_4:30487867-30487886 Msa0324640:CDS 20.0%
!!! TTTTTCTTTCTTAGCATTTA+TGG - chr2_4:30487938-30487957 None:intergenic 20.0%
!!! TATTGTTGTTATTGTTTTAC+TGG + chr2_4:30488540-30488559 Msa0324640:intron 20.0%
! CAAATATCTAAAAGCTTATC+AGG - chr2_4:30485757-30485776 None:intergenic 25.0%
! TACATCAAGAAAAATGAAGA+TGG + chr2_4:30486509-30486528 Msa0324640:CDS 25.0%
! ACATCAAGAAAAATGAAGAT+GGG + chr2_4:30486510-30486529 Msa0324640:CDS 25.0%
!!! AATAAGTTTTTTTTGCACTG+AGG - chr2_4:30486624-30486643 None:intergenic 25.0%
!! GTAAATTAGCAAATTGCTTT+TGG + chr2_4:30486876-30486895 Msa0324640:CDS 25.0%
!! AGAATGGCTTACAAATATTT+TGG + chr2_4:30486914-30486933 Msa0324640:CDS 25.0%
!!! TTACAAATATTTTGGTGATG+TGG + chr2_4:30486922-30486941 Msa0324640:CDS 25.0%
!! AACCAAAAAGTATAGATTGT+TGG - chr2_4:30487021-30487040 None:intergenic 25.0%
!! TGATCAGTGATAATAGTTTT+AGG - chr2_4:30487124-30487143 None:intergenic 25.0%
! TGAATCATCATTATTGTATG+TGG + chr2_4:30487188-30487207 Msa0324640:CDS 25.0%
!!! TTTATACAAGAAAAGTGTTC+AGG + chr2_4:30487650-30487669 Msa0324640:CDS 25.0%
! CTTTCTCTGCAATAAATATT+TGG - chr2_4:30487702-30487721 None:intergenic 25.0%
! GCTAAGAAAGAAAAAAGTAA+AGG + chr2_4:30487943-30487962 Msa0324640:CDS 25.0%
! ATATTGCAATTCAAGCTTTA+CGG + chr2_4:30488088-30488107 Msa0324640:CDS 25.0%
! AATCACAAAACTTGATCAAT+TGG + chr2_4:30488116-30488135 Msa0324640:CDS 25.0%
! GATTTATCAAACTCTTCATT+TGG - chr2_4:30488153-30488172 None:intergenic 25.0%
!!! TGAATTTCCATCTATTTTTC+TGG + chr2_4:30488777-30488796 Msa0324640:three_prime_UTR 25.0%
!!! GGTTTTGATATTTGATTTGT+AGG + chr2_4:30488892-30488911 Msa0324640:three_prime_UTR 25.0%
ATCCTTCCATTTCTTATTGA+AGG + chr2_4:30485576-30485595 Msa0324640:five_prime_UTR 30.0%
TACCTTCAATAAGAAATGGA+AGG - chr2_4:30485581-30485600 None:intergenic 30.0%
TCCATTTCTTATTGAAGGTA+TGG + chr2_4:30485581-30485600 Msa0324640:intron 30.0%
TCCATACCTTCAATAAGAAA+TGG - chr2_4:30485585-30485604 None:intergenic 30.0%
AACAAAGATTGTGAATAGAG+TGG - chr2_4:30485621-30485640 None:intergenic 30.0%
TCACAGTTACTCATCTATTA+TGG + chr2_4:30485687-30485706 Msa0324640:intron 30.0%
TCATCATACATCTACATAAC+AGG + chr2_4:30486102-30486121 Msa0324640:intron 30.0%
TGCATCAATTGAAGATGTAA+AGG + chr2_4:30486286-30486305 Msa0324640:CDS 30.0%
ATGTAAAGGATTACTACGTA+AGG + chr2_4:30486300-30486319 Msa0324640:CDS 30.0%
!!! AAAAGGTTTTAGTTTTCGCA+TGG + chr2_4:30486334-30486353 Msa0324640:CDS 30.0%
!!! AAAGGTTTTAGTTTTCGCAT+GGG + chr2_4:30486335-30486354 Msa0324640:CDS 30.0%
GTTACTAAATCAAGAACGAA+TGG + chr2_4:30486362-30486381 Msa0324640:CDS 30.0%
TCAAGAGATTCTTTGTTCAA+AGG + chr2_4:30486397-30486416 Msa0324640:CDS 30.0%
TTAAAGTACACGATCAAACA+AGG + chr2_4:30486465-30486484 Msa0324640:CDS 30.0%
TAAAGTACACGATCAAACAA+GGG + chr2_4:30486466-30486485 Msa0324640:CDS 30.0%
ATTATGGTCTCTTACAAATC+GGG - chr2_4:30486546-30486565 None:intergenic 30.0%
GATTATGGTCTCTTACAAAT+CGG - chr2_4:30486547-30486566 None:intergenic 30.0%
GGGAAAATAGTACATGATTA+TGG - chr2_4:30486562-30486581 None:intergenic 30.0%
!!! ACACTGATGCTATTTTACTA+GGG - chr2_4:30486673-30486692 None:intergenic 30.0%
!!! AACACTGATGCTATTTTACT+AGG - chr2_4:30486674-30486693 None:intergenic 30.0%
TGAAATGCATAAAAAAACCC+AGG - chr2_4:30486842-30486861 None:intergenic 30.0%
! CACCAACAATCTATACTTTT+TGG + chr2_4:30487016-30487035 Msa0324640:CDS 30.0%
!!! AAACTGAAGAGAGTTTTGTT+TGG + chr2_4:30487059-30487078 Msa0324640:CDS 30.0%
! TATTTCTAATGCAGTTGCAA+AGG + chr2_4:30487153-30487172 Msa0324640:CDS 30.0%
TGATGACTTTGATTCTGATT+GGG + chr2_4:30487312-30487331 Msa0324640:CDS 30.0%
ATTCATACTTTCACTCCTTT+GGG - chr2_4:30487458-30487477 None:intergenic 30.0%
TATTCATACTTTCACTCCTT+TGG - chr2_4:30487459-30487478 None:intergenic 30.0%
!! AAAAGTGTTCAGGATATTTC+AGG + chr2_4:30487660-30487679 Msa0324640:CDS 30.0%
TGAAAAGTCACATGATAGTT+AGG - chr2_4:30487781-30487800 None:intergenic 30.0%
TGTAGTTATTGCAATGGAAA+AGG + chr2_4:30488312-30488331 Msa0324640:CDS 30.0%
! AGATGTCCAAGTTTAAAACA+TGG + chr2_4:30488348-30488367 Msa0324640:CDS 30.0%
!! TACCTTCCATGTTTTAAACT+TGG - chr2_4:30488357-30488376 None:intergenic 30.0%
GGTTTGTAACTGAATAAACT+TGG - chr2_4:30488440-30488459 None:intergenic 30.0%
! GTTACAAACCAATGTCTTTT+AGG + chr2_4:30488450-30488469 Msa0324640:intron 30.0%
ATATCACACCTAAAAGACAT+TGG - chr2_4:30488461-30488480 None:intergenic 30.0%
TTTAGGTGTGATATCATCAA+TGG + chr2_4:30488467-30488486 Msa0324640:intron 30.0%
! CAAACTAGAAGCTTTAACAT+TGG - chr2_4:30488507-30488526 None:intergenic 30.0%
! CAATGTTAAAGCTTCTAGTT+TGG + chr2_4:30488505-30488524 Msa0324640:CDS 30.0%
!! TTCTAGTTTGGTTGATTAAG+AGG + chr2_4:30488517-30488536 Msa0324640:exon 30.0%
CTAGTGTTAATTCCTAGTAT+TGG + chr2_4:30488623-30488642 Msa0324640:intron 30.0%
!! ATTTCCATCTATTTTTCTGG+TGG + chr2_4:30488780-30488799 Msa0324640:three_prime_UTR 30.0%
!! TTTCCATCTATTTTTCTGGT+GGG + chr2_4:30488781-30488800 Msa0324640:three_prime_UTR 30.0%
! CTTTTCAGATTGTGTTTACT+AGG + chr2_4:30488856-30488875 Msa0324640:three_prime_UTR 30.0%
TGTGAAAATGAAGGAGAACA+GGG - chr2_4:30485507-30485526 None:intergenic 35.0%
TTGTGAAAATGAAGGAGAAC+AGG - chr2_4:30485508-30485527 None:intergenic 35.0%
! CCATAATAATAGAGGTGCAT+GGG - chr2_4:30485824-30485843 None:intergenic 35.0%
! ACCATAATAATAGAGGTGCA+TGG - chr2_4:30485825-30485844 None:intergenic 35.0%
ACAACCAGCAAACTAAAGAT+TGG - chr2_4:30485912-30485931 None:intergenic 35.0%
ACATCTACATAACAGGATTG+TGG + chr2_4:30486109-30486128 Msa0324640:intron 35.0%
CATCTACATAACAGGATTGT+GGG + chr2_4:30486110-30486129 Msa0324640:intron 35.0%
AGAGATTCTTTGTTCAAAGG+AGG + chr2_4:30486400-30486419 Msa0324640:CDS 35.0%
GAGATTCTTTGTTCAAAGGA+GGG + chr2_4:30486401-30486420 Msa0324640:CDS 35.0%
TCAAGAAAAATGAAGATGGG+TGG + chr2_4:30486513-30486532 Msa0324640:CDS 35.0%
ATTGATGTGCTTCATGATTC+TGG + chr2_4:30486641-30486660 Msa0324640:CDS 35.0%
AATTGTCAGTTGAAGAATCC+TGG + chr2_4:30486821-30486840 Msa0324640:CDS 35.0%
ATTGTCAGTTGAAGAATCCT+GGG + chr2_4:30486822-30486841 Msa0324640:CDS 35.0%
!!! ATTGCTTTTGGGTTGATTCA+AGG + chr2_4:30486888-30486907 Msa0324640:CDS 35.0%
TGATGATTAACTCCAGTGAA+TGG - chr2_4:30486998-30487017 None:intergenic 35.0%
!!! TTTTTGGTTGTGCTCTGTTA+TGG + chr2_4:30487032-30487051 Msa0324640:CDS 35.0%
!! TTTTGGTTGTGCTCTGTTAT+GGG + chr2_4:30487033-30487052 Msa0324640:CDS 35.0%
CAATAATGATGATTCACCTC+TGG - chr2_4:30487184-30487203 None:intergenic 35.0%
! TAGATTTGGTTCAAGTACTC+TGG - chr2_4:30487232-30487251 None:intergenic 35.0%
CGCTATGTTCATGATAGATT+TGG - chr2_4:30487246-30487265 None:intergenic 35.0%
AGTCATCAGAAGTGATAGTT+TGG - chr2_4:30487300-30487319 None:intergenic 35.0%
CTGATGACTTTGATTCTGAT+TGG + chr2_4:30487311-30487330 Msa0324640:CDS 35.0%
GATGACTTTGATTCTGATTG+GGG + chr2_4:30487313-30487332 Msa0324640:CDS 35.0%
ACGAGTTGCAAGATAACAAA+TGG + chr2_4:30487353-30487372 Msa0324640:CDS 35.0%
AAACTCTGACGTACATAAGA+AGG - chr2_4:30487415-30487434 None:intergenic 35.0%
! TGTATCACAAACTTGCTCAA+TGG - chr2_4:30487514-30487533 None:intergenic 35.0%
GTTTGTGATACAGTATGAGA+AGG + chr2_4:30487522-30487541 Msa0324640:CDS 35.0%
TTTATATCCCATGGAAGAAG+AGG + chr2_4:30487618-30487637 Msa0324640:CDS 35.0%
!! ATATTTCAGGATGAGTTGGT+TGG + chr2_4:30487673-30487692 Msa0324640:CDS 35.0%
!! TATTTCAGGATGAGTTGGTT+GGG + chr2_4:30487674-30487693 Msa0324640:CDS 35.0%
GGCTTAGAACATATTGAGAA+GGG - chr2_4:30487909-30487928 None:intergenic 35.0%
TCTTAGCATTTATGGTCCAT+CGG - chr2_4:30487930-30487949 None:intergenic 35.0%
TCTCTTGAACTATCTGAAAG+AGG + chr2_4:30488042-30488061 Msa0324640:CDS 35.0%
TTTACGAATGAAAGGCAGTT+TGG + chr2_4:30488263-30488282 Msa0324640:CDS 35.0%
ACCAGTTGTAGTTATTGCAA+TGG + chr2_4:30488306-30488325 Msa0324640:CDS 35.0%
TCCATTGCAATAACTACAAC+TGG - chr2_4:30488310-30488329 None:intergenic 35.0%
! GTCCAAGTTTAAAACATGGA+AGG + chr2_4:30488352-30488371 Msa0324640:CDS 35.0%
GTAACTGAATAAACTTGGCA+TGG - chr2_4:30488435-30488454 None:intergenic 35.0%
!!! AGACCTGTTTTTGCCAATAA+GGG + chr2_4:30488654-30488673 Msa0324640:intron 35.0%
TATCCCTTATTGGCAAAAAC+AGG - chr2_4:30488660-30488679 None:intergenic 35.0%
TCGGCAAAATTATCCCTTAT+TGG - chr2_4:30488670-30488689 None:intergenic 35.0%
! GATAATTTTGCCGAAGTCAA+AGG + chr2_4:30488676-30488695 Msa0324640:three_prime_UTR 35.0%
GATTTCTCTGATGTGATTCT+AGG + chr2_4:30488704-30488723 Msa0324640:three_prime_UTR 35.0%
ATTCCCACCAGAAAAATAGA+TGG - chr2_4:30488787-30488806 None:intergenic 35.0%
TTCAGATTGTGTTTACTAGG+AGG + chr2_4:30488859-30488878 Msa0324640:three_prime_UTR 35.0%
TCAGATTGTGTTTACTAGGA+GGG + chr2_4:30488860-30488879 Msa0324640:three_prime_UTR 35.0%
! AGTGATGGGAATTTGATACG+TGG - chr2_4:30485660-30485679 None:intergenic 40.0%
ACTGTGAAGAACTGAGTGAT+GGG - chr2_4:30485674-30485693 None:intergenic 40.0%
AACTGTGAAGAACTGAGTGA+TGG - chr2_4:30485675-30485694 None:intergenic 40.0%
! AATAATAGAGGTGCATGGGA+TGG - chr2_4:30485820-30485839 None:intergenic 40.0%
CCCATGCACCTCTATTATTA+TGG + chr2_4:30485821-30485840 Msa0324640:intron 40.0%
!! ACCTCCAATCTTTAGTTTGC+TGG + chr2_4:30485905-30485924 Msa0324640:intron 40.0%
ACCAGCAAACTAAAGATTGG+AGG - chr2_4:30485909-30485928 None:intergenic 40.0%
!! TTTAGTTTGCTGGTTGTCGT+GGG + chr2_4:30485915-30485934 Msa0324640:intron 40.0%
CATGAACATACCACCATGAA+TGG + chr2_4:30486167-30486186 Msa0324640:CDS 40.0%
TACATCATCTGAGCCATTCA+TGG - chr2_4:30486183-30486202 None:intergenic 40.0%
! ATTCCTTCAAAGGGCTCTAA+GGG - chr2_4:30486260-30486279 None:intergenic 40.0%
GCAAACTCCATTCCTTCAAA+GGG - chr2_4:30486269-30486288 None:intergenic 40.0%
TGCAAACTCCATTCCTTCAA+AGG - chr2_4:30486270-30486289 None:intergenic 40.0%
GTAAGGTACGCCAAGAAAAA+AGG + chr2_4:30486317-30486336 Msa0324640:CDS 40.0%
!! AGAACGAATGGTGTTGTGAT+TGG + chr2_4:30486374-30486393 Msa0324640:CDS 40.0%
AAGATGCTGTGAACTTCTTG+GGG - chr2_4:30486582-30486601 None:intergenic 40.0%
GAAGATGCTGTGAACTTCTT+GGG - chr2_4:30486583-30486602 None:intergenic 40.0%
GTGCTTCATGATTCTGGTAT+AGG + chr2_4:30486647-30486666 Msa0324640:CDS 40.0%
TCAGTGTTATGTACTGAGAG+TGG + chr2_4:30486686-30486705 Msa0324640:CDS 40.0%
!! AGTGGTGGTGTTGATAATGT+TGG + chr2_4:30486704-30486723 Msa0324640:CDS 40.0%
! GGTTGATTCAAGGTCTAGAA+TGG + chr2_4:30486898-30486917 Msa0324640:CDS 40.0%
!! TGAATGGAACAAAGGGCATT+AGG - chr2_4:30486982-30487001 None:intergenic 40.0%
ACTCCAGTGAATGGAACAAA+GGG - chr2_4:30486989-30487008 None:intergenic 40.0%
AACTCCAGTGAATGGAACAA+AGG - chr2_4:30486990-30487009 None:intergenic 40.0%
TTGTTTGGTTGCTACGTACT+TGG + chr2_4:30487074-30487093 Msa0324640:CDS 40.0%
ACGTACTTGGTTAGAAGCAA+TGG + chr2_4:30487087-30487106 Msa0324640:CDS 40.0%
ACTTGGTTAGAAGCAATGGA+TGG + chr2_4:30487091-30487110 Msa0324640:CDS 40.0%
! AGTTGCAAAGGTTTTTCCAG+AGG + chr2_4:30487165-30487184 Msa0324640:CDS 40.0%
AAGAAGATCTACACCATTCG+AGG + chr2_4:30487379-30487398 Msa0324640:CDS 40.0%
TCTACACCATTCGAGGAAAA+TGG + chr2_4:30487386-30487405 Msa0324640:CDS 40.0%
CTACACCATTCGAGGAAAAT+GGG + chr2_4:30487387-30487406 Msa0324640:CDS 40.0%
TCTTGACGCACGTTATAACA+AGG + chr2_4:30487546-30487565 Msa0324640:CDS 40.0%
GAGAGAAGAATGTCAAGACA+AGG + chr2_4:30487572-30487591 Msa0324640:CDS 40.0%
GGATATAAAGTACGCAGAAG+TGG - chr2_4:30487607-30487626 None:intergenic 40.0%
TCTGCGTACTTTATATCCCA+TGG + chr2_4:30487609-30487628 Msa0324640:CDS 40.0%
! TTTGAAGCCTCTTCTTCCAT+GGG - chr2_4:30487628-30487647 None:intergenic 40.0%
!!! TTTTGAAGCCTCTTCTTCCA+TGG - chr2_4:30487629-30487648 None:intergenic 40.0%
CAGGATATTTCAGGATGAGT+TGG + chr2_4:30487669-30487688 Msa0324640:CDS 40.0%
! TTGTCGCATGTTTGAGTTTG+TGG + chr2_4:30487828-30487847 Msa0324640:CDS 40.0%
! TGTCGCATGTTTGAGTTTGT+GGG + chr2_4:30487829-30487848 Msa0324640:CDS 40.0%
CGGCTTAGAACATATTGAGA+AGG - chr2_4:30487910-30487929 None:intergenic 40.0%
! CTCAATATGTTCTAAGCCGA+TGG + chr2_4:30487911-30487930 Msa0324640:CDS 40.0%
AATTGCAATTGAAGACCTCG+AGG + chr2_4:30487966-30487985 Msa0324640:CDS 40.0%
TGCAATTCAAGCTTTACGGA+AGG + chr2_4:30488092-30488111 Msa0324640:CDS 40.0%
CAAGTTTATGGTCTGGTCAT+CGG - chr2_4:30488202-30488221 None:intergenic 40.0%
GATCACACAAGTTTATGGTC+TGG - chr2_4:30488209-30488228 None:intergenic 40.0%
TGGAGGATCACACAAGTTTA+TGG - chr2_4:30488214-30488233 None:intergenic 40.0%
! TTGGACATCTACGTTTGTTG+TGG - chr2_4:30488338-30488357 None:intergenic 40.0%
GTCTGCAACAAACCAATACT+AGG - chr2_4:30488638-30488657 None:intergenic 40.0%
!!! CAGACCTGTTTTTGCCAATA+AGG + chr2_4:30488653-30488672 Msa0324640:intron 40.0%
! AATTTTGCCGAAGTCAAAGG+TGG + chr2_4:30488679-30488698 Msa0324640:three_prime_UTR 40.0%
! AAATCCACCACCTTTGACTT+CGG - chr2_4:30488689-30488708 None:intergenic 40.0%
TCTGATGTGATTCTAGGCTT+TGG + chr2_4:30488710-30488729 Msa0324640:three_prime_UTR 40.0%
! TTTCCCAGCTTTTGTACCTT+TGG + chr2_4:30488806-30488825 Msa0324640:three_prime_UTR 40.0%
CAGATTGTGTTTACTAGGAG+GGG + chr2_4:30488861-30488880 Msa0324640:three_prime_UTR 40.0%
CAGAGCGGTTGTGAAAATGA+AGG - chr2_4:30485516-30485535 None:intergenic 45.0%
GTCGAGAATCAAGTACAGAG+CGG - chr2_4:30485531-30485550 None:intergenic 45.0%
GCAACAGAGAGTTGCAGATT+CGG - chr2_4:30485556-30485575 None:intergenic 45.0%
!! CTTTAGTTTGCTGGTTGTCG+TGG + chr2_4:30485914-30485933 Msa0324640:intron 45.0%
ATCATCTGAGCCATTCATGG+TGG - chr2_4:30486180-30486199 None:intergenic 45.0%
!! TTCCTTCAAAGGGCTCTAAG+GGG - chr2_4:30486259-30486278 None:intergenic 45.0%
CATTCCTTCAAAGGGCTCTA+AGG - chr2_4:30486261-30486280 None:intergenic 45.0%
! CTTAGAGCCCTTTGAAGGAA+TGG + chr2_4:30486259-30486278 Msa0324640:CDS 45.0%
GTACACGATCAAACAAGGGT+AGG + chr2_4:30486470-30486489 Msa0324640:CDS 45.0%
CGAAGATGCTGTGAACTTCT+TGG - chr2_4:30486584-30486603 None:intergenic 45.0%
GTGTTATGTACTGAGAGTGG+TGG + chr2_4:30486689-30486708 Msa0324640:CDS 45.0%
GCATTTCAAATGGACGCAGA+AGG + chr2_4:30486854-30486873 Msa0324640:CDS 45.0%
ATGCCCTTTGTTCCATTCAC+TGG + chr2_4:30486983-30487002 Msa0324640:CDS 45.0%
! AGGAACCCATTTTCCTCGAA+TGG - chr2_4:30487395-30487414 None:intergenic 45.0%
! GTACGTCAGAGTTTTTGTGC+TGG + chr2_4:30487421-30487440 Msa0324640:CDS 45.0%
CATACTTTCACTCCTTTGGG+TGG - chr2_4:30487455-30487474 None:intergenic 45.0%
CCTTTCATTCGTAAAGTGCG+TGG - chr2_4:30488258-30488277 None:intergenic 45.0%
CCACGCACTTTACGAATGAA+AGG + chr2_4:30488255-30488274 Msa0324640:CDS 45.0%
!!! TCTGGCGTGATTTCAGGTTT+TGG + chr2_4:30488740-30488759 Msa0324640:three_prime_UTR 45.0%
!!! TCAGGTTTTGGATGCAGTCT+TGG + chr2_4:30488752-30488771 Msa0324640:three_prime_UTR 45.0%
! GCACCAAAGGTACAAAAGCT+GGG - chr2_4:30488812-30488831 None:intergenic 45.0%
! CAGCTTTTGTACCTTTGGTG+CGG + chr2_4:30488811-30488830 Msa0324640:three_prime_UTR 45.0%
TTACTAGGAGGGGATCTGTT+CGG + chr2_4:30488871-30488890 Msa0324640:three_prime_UTR 45.0%
ACTTTCACTCCTTTGGGTGG+TGG - chr2_4:30487452-30487471 None:intergenic 50.0%
GCAATTGAAGACCTCGAGGA+AGG + chr2_4:30487970-30487989 Msa0324640:CDS 50.0%
GTCTGGTCATCGGAGACTTT+AGG - chr2_4:30488192-30488211 None:intergenic 50.0%
AAGTGCGTGGACGACCTTTA+GGG - chr2_4:30488245-30488264 None:intergenic 50.0%
AAAGTGCGTGGACGACCTTT+AGG - chr2_4:30488246-30488265 None:intergenic 50.0%
TTTGCCGAAGTCAAAGGTGG+TGG + chr2_4:30488682-30488701 Msa0324640:three_prime_UTR 50.0%
!! CTAGGCTTTGGATGCACTTC+TGG + chr2_4:30488722-30488741 Msa0324640:three_prime_UTR 50.0%
TGCACTTCTGGCGTGATTTC+AGG + chr2_4:30488734-30488753 Msa0324640:three_prime_UTR 50.0%
! CGCACCAAAGGTACAAAAGC+TGG - chr2_4:30488813-30488832 None:intergenic 50.0%
! TCTAAGGGGAGCTCTGATGG+CGG - chr2_4:30486245-30486264 None:intergenic 55.0%
CTCCCCTTAGAGCCCTTTGA+AGG + chr2_4:30486254-30486273 Msa0324640:CDS 55.0%
CTGGAATGTCCACCACCCAA+AGG + chr2_4:30487440-30487459 Msa0324640:CDS 55.0%
!! CTCTGTTTCTGCCTTCCTCG+AGG - chr2_4:30487984-30488003 None:intergenic 55.0%
CTTGTGTGATCCTCCACGTG+CGG + chr2_4:30488218-30488237 Msa0324640:CDS 55.0%
!! GGCTCTAAGGGGAGCTCTGA+TGG - chr2_4:30486248-30486267 None:intergenic 60.0%
! GTACCTTTGGTGCGGCTGTG+CGG + chr2_4:30488819-30488838 Msa0324640:three_prime_UTR 60.0%
! TACCTTTGGTGCGGCTGTGC+GGG + chr2_4:30488820-30488839 Msa0324640:three_prime_UTR 60.0%
! CCTTTAGGGACCGCACGTGG+AGG - chr2_4:30488231-30488250 None:intergenic 65.0%
CCTCCACGTGCGGTCCCTAA+AGG + chr2_4:30488228-30488247 Msa0324640:CDS 65.0%
! CGACCTTTAGGGACCGCACG+TGG - chr2_4:30488234-30488253 None:intergenic 65.0%
AACCCGCACAGCCGCACCAA+AGG - chr2_4:30488825-30488844 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 30485492 30489103 30485492 ID=Msa0324640;Name=Msa0324640
chr2_4 mRNA 30485492 30489103 30485492 ID=Msa0324640-mRNA-1;Parent=Msa0324640;Name=Msa0324640-mRNA-1;_AED=0.03;_eAED=0.03;_QI=122|1|1|1|0.33|0|4|451|765
chr2_4 exon 30485492 30485597 30485492 ID=Msa0324640-mRNA-1:exon:10224;Parent=Msa0324640-mRNA-1
chr2_4 exon 30486124 30488373 30486124 ID=Msa0324640-mRNA-1:exon:10225;Parent=Msa0324640-mRNA-1
chr2_4 exon 30488472 30488538 30488472 ID=Msa0324640-mRNA-1:exon:10226;Parent=Msa0324640-mRNA-1
chr2_4 exon 30488656 30489103 30488656 ID=Msa0324640-mRNA-1:exon:10227;Parent=Msa0324640-mRNA-1
chr2_4 five_prime_UTR 30485492 30485597 30485492 ID=Msa0324640-mRNA-1:five_prime_utr;Parent=Msa0324640-mRNA-1
chr2_4 five_prime_UTR 30486124 30486139 30486124 ID=Msa0324640-mRNA-1:five_prime_utr;Parent=Msa0324640-mRNA-1
chr2_4 CDS 30486140 30488373 30486140 ID=Msa0324640-mRNA-1:cds;Parent=Msa0324640-mRNA-1
chr2_4 CDS 30488472 30488535 30488472 ID=Msa0324640-mRNA-1:cds;Parent=Msa0324640-mRNA-1
chr2_4 three_prime_UTR 30488536 30488538 30488536 ID=Msa0324640-mRNA-1:three_prime_utr;Parent=Msa0324640-mRNA-1
chr2_4 three_prime_UTR 30488656 30489103 30488656 ID=Msa0324640-mRNA-1:three_prime_utr;Parent=Msa0324640-mRNA-1
Gene Sequence

>Msa0324640

ATGACTGATCAAGAAATGACAGATATGCATGAACATACCACCATGAATGGCTCAGATGATGTACTTGCAAATGCTATTGATGCAGATTCTCTCAATGATAGACCGCCATCAGAGCTCCCCTTAGAGCCCTTTGAAGGAATGGAGTTTGCATCAATTGAAGATGTAAAGGATTACTACGTAAGGTACGCCAAGAAAAAAGGTTTTAGTTTTCGCATGGGTCGTGTTACTAAATCAAGAACGAATGGTGTTGTGATTGGTCAAGAGATTCTTTGTTCAAAGGAGGGTTTTAGATCCAAAAAAAAATTGAAAAAAGAAAATGGTAGTCTTAAAGTACACGATCAAACAAGGGTAGGATGCAAGTCAATGCTGTACATCAAGAAAAATGAAGATGGGTGGATTGTTTCCCGATTTGTAAGAGACCATAATCATGTACTATTTTCCCCAAGAAGTTCACAGCATCTTCGAGTACATAGAAAGAAAACCTCAGTGCAAAAAAAACTTATTGATGTGCTTCATGATTCTGGTATAGGCCCTAGTAAAATAGCATCAGTGTTATGTACTGAGAGTGGTGGTGTTGATAATGTTGGATTTAGCCAACAAGATGTTATTAATTATTTAAGTGTAAAGAGACAAAAACAATTAGAAAATGGAGATGCTCACTTGATGTTATCGTACTTTAAGAATTGTCAGTTGAAGAATCCTGGGTTTTTTTATGCATTTCAAATGGACGCAGAAGGTAAATTAGCAAATTGCTTTTGGGTTGATTCAAGGTCTAGAATGGCTTACAAATATTTTGGTGATGTGGTGACTTTTGATCCAACATACTTAACAAATAAATACCTAATGCCCTTTGTTCCATTCACTGGAGTTAATCATCACCAACAATCTATACTTTTTGGTTGTGCTCTGTTATGGGATGAAACTGAAGAGAGTTTTGTTTGGTTGCTACGTACTTGGTTAGAAGCAATGGATGGAGTTAGTCCTAAAACTATTATCACTGATCAAGACACTTCTATTTCTAATGCAGTTGCAAAGGTTTTTCCAGAGGTGAATCATCATTATTGTATGTGGCATATTGAGAAGAAAGTTCCAGAGTACTTGAACCAAATCTATCATGAACATAGCGAGTTTAAAAATCAATTTCACAAGTGCATTCACCAAACTATCACTTCTGATGACTTTGATTCTGATTGGGGAGCAATGATAGATAAGTACGAGTTGCAAGATAACAAATGGCTAAAGAAGATCTACACCATTCGAGGAAAATGGGTTCCTTCTTATGTACGTCAGAGTTTTTGTGCTGGAATGTCCACCACCCAAAGGAGTGAAAGTATGAATAAATTTTTTAAGGGATTTTTGAATTCAAGTACTCCATTGAGCAAGTTTGTGATACAGTATGAGAAGGCTCTTGACGCACGTTATAACAAGGAAAGAGAGAAGAATGTCAAGACAAGGAATTCAAAACCACTTCTGCGTACTTTATATCCCATGGAAGAAGAGGCTTCAAAAATTTATACAAGAAAAGTGTTCAGGATATTTCAGGATGAGTTGGTTGGGTCCCAAATATTTATTGCAGAGAAAGTTAAGTTTTTTGATGAAGTTTCCACATACAAAATTCATGAAATTTACAAAGAAAAACCTAACTATCATGTGACTTTTCAGATCATTTCAAAAGAAGCAAAATGTAGTTGTCGCATGTTTGAGTTTGTGGGAATTCTCTGTAGACATGTATTAAACGTATTCATAAAGGAAAATGTCTATTCTCTCCCTTCTCAATATGTTCTAAGCCGATGGACCATAAATGCTAAGAAAGAAAAAAGTAAAGGAATTGCAATTGAAGACCTCGAGGAAGGCAGAAACAGAGCTTCAAGTACTTCATTATTTAATAGTATTATGGTTGAGTCTCTTGAACTATCTGAAAGAGGTTCACGATCAGAAAAGCATCATGATATTGCAATTCAAGCTTTACGGAAGGCAATCACAAAACTTGATCAATTGGATCTTGAAGAACCAAATGAAGAGTTTGATAAATCGACATTTGAAGTTATGCCTAAAGTCTCCGATGACCAGACCATAAACTTGTGTGATCCTCCACGTGCGGTCCCTAAAGGTCGTCCACGCACTTTACGAATGAAAGGCAGTTTGGAATTGCACAAGAAAAATTCAACCAGTTGTAGTTATTGCAATGGAAAAGGCCACAACAAACGTAGATGTCCAAGTTTAAAACATGGAAGGTGTGATATCATCAATGGTCAACTTTCAAATTCCAATGTTAAAGCTTCTAGTTTGGTTGATTAA
Protein sequence

>Msa0324640

MTDQEMTDMHEHTTMNGSDDVLANAIDADSLNDRPPSELPLEPFEGMEFASIEDVKDYYVRYAKKKGFSFRMGRVTKSRTNGVVIGQEILCSKEGFRSKKKLKKENGSLKVHDQTRVGCKSMLYIKKNEDGWIVSRFVRDHNHVLFSPRSSQHLRVHRKKTSVQKKLIDVLHDSGIGPSKIASVLCTESGGVDNVGFSQQDVINYLSVKRQKQLENGDAHLMLSYFKNCQLKNPGFFYAFQMDAEGKLANCFWVDSRSRMAYKYFGDVVTFDPTYLTNKYLMPFVPFTGVNHHQQSILFGCALLWDETEESFVWLLRTWLEAMDGVSPKTIITDQDTSISNAVAKVFPEVNHHYCMWHIEKKVPEYLNQIYHEHSEFKNQFHKCIHQTITSDDFDSDWGAMIDKYELQDNKWLKKIYTIRGKWVPSYVRQSFCAGMSTTQRSESMNKFFKGFLNSSTPLSKFVIQYEKALDARYNKEREKNVKTRNSKPLLRTLYPMEEEASKIYTRKVFRIFQDELVGSQIFIAEKVKFFDEVSTYKIHEIYKEKPNYHVTFQIISKEAKCSCRMFEFVGILCRHVLNVFIKENVYSLPSQYVLSRWTINAKKEKSKGIAIEDLEEGRNRASSTSLFNSIMVESLELSERGSRSEKHHDIAIQALRKAITKLDQLDLEEPNEEFDKSTFEVMPKVSDDQTINLCDPPRAVPKGRPRTLRMKGSLELHKKNSTSCSYCNGKGHNKRRCPSLKHGRCDIINGQLSNSNVKASSLVD*