AlfalfaGEDB | Msa0012850

M. sativa cultivar zhongmu-4 / Msa0012850

KEGG: K03595
GO: GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0043021 GO:0043022 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0097177
PFAM: PF01926.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0012850	sp\|O82626\|ERG_ANTMA	59.662	414	147	7	17	412	12	423	4.52e-167	478

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001310	Msa0012850	0.807314	5.371140e-50	-8.615850e-47
Msa0012850	Msa0489410	0.804102	2.538267e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0012850	MtrunA17_Chr5g0404241	91.860	430	8	5	1	412	1	421	0.0	752
Msa0012850	MtrunA17_Chr6g0459721	90.465	430	8	6	1	412	1	415	0.0	737
Msa0012850	MtrunA17_Chr1g0161851	83.495	412	18	4	1	412	1	362	0.0	668
Msa0012850	MtrunA17_Chr8g0372901	23.684	266	193	5	147	412	139	394	6.91e-14	72.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0012850	AT1G30960.1	59.193	446	136	8	4	412	1	437	4.79e-161	460

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Find 131 sgRNAs with CRISPR-Local

Find 535 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGTGAGATACGAGTT+TGG	0.461973	1_1:-19578101	Msa0012850:three_prime_UTR
AAAATTGAGTTCCTAGACTC+CGG	0.604158	1_1:+19578250	None:intergenic
AACCTATGTTCAATATCGTA+TGG	0.502441	1_1:+19579094	None:intergenic
AAGAAAAGCCGAAGGTGACG+TGG	0.574647	1_1:-19585404	Msa0012850:CDS
AAGAAACAGGTTGAGTTCGT+TGG	0.544765	1_1:-19578317	Msa0012850:three_prime_UTR
AAGATCTTCCTGGATTTGAA+AGG	0.540512	1_1:-19579789	Msa0012850:intron
AAGATTCTTGTGGGGAAGCG+TGG	0.571176	1_1:-19578995	Msa0012850:CDS
AAGGTAGCGTCTGAATGTTC+GGG	0.446165	1_1:+19585471	None:intergenic
AATAGGCAGTAGAACGGCCC+TGG	0.484531	1_1:-19579320	Msa0012850:intron
ACACATTTATTTAGGGCATT+TGG	0.320156	1_1:-19578131	Msa0012850:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTAAATGAACACAACTGTTT+CGG	-	chr1_1:19579448-19579467	Msa0012850:intron	30.0%
ACTAAGTTTCTTAGTAAGGT+GGG	+	chr1_1:19579566-19579585	None:intergenic	30.0%
TCATGTAACCCATTCTTATA+GGG	+	chr1_1:19579642-19579661	None:intergenic	30.0%
GTTTAAATAGGCAAGCTATT+CGG	-	chr1_1:19580004-19580023	Msa0012850:intron	30.0%
AAGCTGAGTTCAATTTATGT+GGG	-	chr1_1:19580136-19580155	Msa0012850:intron	30.0%
GGTTAAAGATTTGTCTAGAA+GGG	-	chr1_1:19580417-19580436	Msa0012850:intron	30.0%
CAAATCCTTTCCATTATTGA+GGG	+	chr1_1:19580465-19580484	None:intergenic	30.0%
GATAAACCATCATTCCATAT+CGG	+	chr1_1:19580527-19580546	None:intergenic	30.0%
TCTGATTTATCATCATAGGT+TGG	-	chr1_1:19580983-19581002	Msa0012850:intron	30.0%
TTTGCATCTTAGTCAGAATA+CGG	-	chr1_1:19581229-19581248	Msa0012850:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	19578063	19585625	19578063	ID=Msa0012850;Name=Msa0012850
chr1_1	mRNA	19578063	19585625	19578063	ID=Msa0012850-mRNA-1;Parent=Msa0012850;Name=Msa0012850-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0\|0.71\|0.75\|1\|0.85\|0.75\|8\|450\|412
chr1_1	exon	19578063	19578597	19578063	ID=Msa0012850-mRNA-1:exon:7083;Parent=Msa0012850-mRNA-1
chr1_1	exon	19578983	19579122	19578983	ID=Msa0012850-mRNA-1:exon:7082;Parent=Msa0012850-mRNA-1
chr1_1	exon	19579230	19579337	19579230	ID=Msa0012850-mRNA-1:exon:7081;Parent=Msa0012850-mRNA-1
chr1_1	exon	19579589	19579661	19579589	ID=Msa0012850-mRNA-1:exon:7080;Parent=Msa0012850-mRNA-1
chr1_1	exon	19579790	19579948	19579790	ID=Msa0012850-mRNA-1:exon:7079;Parent=Msa0012850-mRNA-1
chr1_1	exon	19581455	19581600	19581455	ID=Msa0012850-mRNA-1:exon:7078;Parent=Msa0012850-mRNA-1
chr1_1	exon	19582598	19582687	19582598	ID=Msa0012850-mRNA-1:exon:7077;Parent=Msa0012850-mRNA-1
chr1_1	exon	19585188	19585625	19585188	ID=Msa0012850-mRNA-1:exon:7076;Parent=Msa0012850-mRNA-1
chr1_1	CDS	19585188	19585625	19585188	ID=Msa0012850-mRNA-1:cds;Parent=Msa0012850-mRNA-1
chr1_1	CDS	19582598	19582687	19582598	ID=Msa0012850-mRNA-1:cds;Parent=Msa0012850-mRNA-1
chr1_1	CDS	19581455	19581600	19581455	ID=Msa0012850-mRNA-1:cds;Parent=Msa0012850-mRNA-1
chr1_1	CDS	19579790	19579948	19579790	ID=Msa0012850-mRNA-1:cds;Parent=Msa0012850-mRNA-1
chr1_1	CDS	19579589	19579661	19579589	ID=Msa0012850-mRNA-1:cds;Parent=Msa0012850-mRNA-1
chr1_1	CDS	19579230	19579337	19579230	ID=Msa0012850-mRNA-1:cds;Parent=Msa0012850-mRNA-1
chr1_1	CDS	19578983	19579122	19578983	ID=Msa0012850-mRNA-1:cds;Parent=Msa0012850-mRNA-1
chr1_1	CDS	19578513	19578597	19578513	ID=Msa0012850-mRNA-1:cds;Parent=Msa0012850-mRNA-1
chr1_1	three_prime_UTR	19578063	19578512	19578063	ID=Msa0012850-mRNA-1:three_prime_utr;Parent=Msa0012850-mRNA-1

Gene Sequence

>Msa0012850

ATGAAATCCTTAAGAATCTTCAAAAACACTCTCTATTCAACTTCTTCACCCAAAATCACCACCACCACAACCACCACAATCTTCCGCGCTTTCTTCTCCGCCGAACCCCTCACACCGGAACCCGATCCTCAACCCGAACATTCAGACGCTACCTTCGATAGTTTTCACTACGAACTCCCAATTCTCAACAACAACGACGAAGAAAAGCCGAAGGTGACGTGGAATAAGAAATACCGAGACCGGACGGATAAACTTGTGTTCGGCGATGAGCCGAAGGGGAGAGTGAAGTTTAAGGAGGAAGAGGAGGAGAGGAAAACTAAGGCGCTTGAGAAGGCTTTGTTGGAAGCTGCGTTGAGTAAACCCGAGGAGGAGGAGGAGGAGGAGGAGGAAGATGAAGATGTCGGTGTTGTGAATGAGGAGGATCAGAAGTCTTTGTCTGTTGGAACAAAGGTGGCGGCTGTTTCACGCAAGACAAACACCACAACTCATGAAGTTGTGGGCGTGTTGACTAAAGGAGACACCCAAATTTGTTTTTTTGATACGCCAGGGCTTGTGCTAAATTGTAGTGGATTCCCTTATAAGGATGCCAAGGCTCGTGTTGAAAGTGCTTGGAGTTCAGTCAATCTCTATGAAGTGCTCATAGTCATTTTTGATGTCCATAGACATATTACTAGGCCTGATTCAAGAGTTGTAAAACTGATCAAGCGAATGGGAGAACGGTCCATTCCAAATCAAAAGCGAGTTTTGTGTATGAATAAGATTGATTTAGTAGAGAAAAAGAAAGACTTAACAAAGGTTGCTGAAGAATTCGAAGATCTTCCTGGATTTGAAAGGCGTTTCATGATATCTGGAATGAAGGGGGCTGGCGTAAAAGATTTGACTCAGTTCTTGATGGAGCAGGCATGTGCAGTAGAACGGCCCTGGGAAGAAGATCCATTTACCATGACCGAGGAAGTTATGAAAATGATATCATTAGAAGTTGTGCGTGAAAGGTTGTTGGACCATGTACATCAGGAAATTCCATACGATATTGAACATAGGTTGATTGACTGGAAAGAGTTGCGAGATGGTTCTCTTAGGATTGAACAGCACTTTCTCACTAACAAATTAGGCCAACTCAAGATTCTTGTGGGGAAGCGTGGCTCAAAAATTGGGAGAATAGGAATAGAAGCTAATGAAGAGCTAAGGACCATTTTCAAAAAGCAAGTGCATCTAGTTCTTCAGGTTAAGCTCAAATGA

Protein sequence

>Msa0012850

MKSLRIFKNTLYSTSSPKITTTTTTTIFRAFFSAEPLTPEPDPQPEHSDATFDSFHYELPILNNNDEEKPKVTWNKKYRDRTDKLVFGDEPKGRVKFKEEEEERKTKALEKALLEAALSKPEEEEEEEEEDEDVGVVNEEDQKSLSVGTKVAAVSRKTNTTTHEVVGVLTKGDTQICFFDTPGLVLNCSGFPYKDAKARVESAWSSVNLYEVLIVIFDVHRHITRPDSRVVKLIKRMGERSIPNQKRVLCMNKIDLVEKKKDLTKVAEEFEDLPGFERRFMISGMKGAGVKDLTQFLMEQACAVERPWEEDPFTMTEEVMKMISLEVVRERLLDHVHQEIPYDIEHRLIDWKELRDGSLRIEQHFLTNKLGQLKILVGKRGSKIGRIGIEANEELRTIFKKQVHLVLQVKLK*