AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0039730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0039730 MtrunA17_Chr1g0204351 94.694 245 12 1 1 245 1 244 1.62e-171 472
Msa0039730 MtrunA17_Chr6g0474671 52.632 228 98 6 21 243 16 238 3.84e-67 207
Msa0039730 MtrunA17_Chr6g0474681 39.106 179 99 3 72 244 2 176 1.30e-33 120
Msa0039730 MtrunA17_Chr1g0204361 36.264 182 100 2 61 231 4 180 4.77e-31 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0039730 AT5G51780.2 44.253 174 87 2 74 237 23 196 6.51e-36 126
Msa0039730 AT5G51780.1 44.186 172 86 2 76 237 2 173 1.68e-35 124
Msa0039730 AT4G25400.1 43.275 171 90 3 70 237 19 185 1.83e-34 122
Msa0039730 AT4G25410.1 43.114 167 81 5 80 234 47 211 1.57e-32 118
Msa0039730 AT5G51790.1 40.642 187 95 6 62 234 9 193 2.21e-32 117
Msa0039730 AT4G25400.2 45.098 153 77 3 70 219 19 167 5.84e-32 116
Msa0039730 AT5G51790.3 40.642 187 95 6 62 234 74 258 7.58e-32 118
Msa0039730 AT5G51790.4 41.860 172 84 6 62 219 9 178 8.61e-31 113
Msa0039730 AT5G51790.2 41.860 172 84 6 62 219 74 243 2.91e-30 113
Msa0039730 AT1G62975.1 31.156 199 113 3 63 237 61 259 6.58e-23 94.4
Msa0039730 AT1G62975.2 33.529 170 89 3 74 219 72 241 1.05e-20 88.2
Msa0039730 AT1G12540.1 34.637 179 102 5 76 240 75 252 1.29e-20 88.2

Find 59 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCCCACTTTCTAAATTTC+TGG 0.103688 1_1:-69669591 Msa0039730:CDS
TCAAAGACTTTCCTAATTAT+TGG 0.193329 1_1:+69670264 None:intergenic
CAAAATTAATTGTGAAGTTT+AGG 0.202471 1_1:-69669084 Msa0039730:three_prime_UTR
GAAGAGGGAGAAGGGATCTA+AGG 0.225427 1_1:+69669935 None:intergenic
TTCCTTAGCCACCAATAATT+AGG 0.273744 1_1:-69670275 Msa0039730:five_prime_UTR
TTGGTTTCTGCTTCTGTCTT+GGG 0.275198 1_1:-69669156 Msa0039730:three_prime_UTR
GAATCCATGTGGGCTCTCTT+TGG 0.295811 1_1:-69669175 Msa0039730:three_prime_UTR
TATTGAAGATAAGAAGAAAA+TGG 0.318828 1_1:-69670013 Msa0039730:CDS
TTTGGTTTCTGCTTCTGTCT+TGG 0.319200 1_1:-69669157 Msa0039730:three_prime_UTR
TTATATTCTCTCTTTCTAAC+TGG 0.333136 1_1:-69669246 Msa0039730:three_prime_UTR
TCTATATAAAATAATTTGTT+TGG 0.336326 1_1:+69670066 None:intergenic
ATAAGAAGAAAATGGTTCAT+AGG 0.350975 1_1:-69670005 Msa0039730:CDS
ACTAAATGATGGATTCATAT+TGG 0.351963 1_1:+69669322 None:intergenic
AAATCACTTGAAGAAGAATA+TGG 0.357120 1_1:-69669765 Msa0039730:CDS
CGTTGTAAAGGAAACATGTT+TGG 0.371777 1_1:+69670231 None:intergenic
TAAAGGAAACATGTTTGGTT+TGG 0.398708 1_1:+69670236 None:intergenic
TTCCTAATTATTGGTGGCTA+AGG 0.406014 1_1:+69670273 None:intergenic
GAATCCATCATTTAGTTGTT+CGG 0.416250 1_1:-69669315 Msa0039730:CDS
TATATAGATCGTACAAATCA+TGG 0.422086 1_1:-69670051 Msa0039730:CDS
GTGGTTGAGACTATTAGATA+AGG 0.433605 1_1:+69670188 None:intergenic
TGGAAGAACTAAGTTACAAA+AGG 0.434554 1_1:-69669745 Msa0039730:CDS
CCACAGTTCTATTATTCTTG+TGG 0.455790 1_1:+69669654 None:intergenic
ATATAAAATAATTTGTTTGG+TGG 0.460635 1_1:+69670069 None:intergenic
AAGAGGGAGAAGGGATCTAA+GGG 0.467872 1_1:+69669936 None:intergenic
TAAGAAGAAAATGGTTCATA+GGG 0.475156 1_1:-69670004 Msa0039730:CDS
TAGATCTAACTGAGCTAAGA+AGG 0.478104 1_1:-69669352 Msa0039730:CDS
TAATCCGAACAACTAAATGA+TGG 0.483323 1_1:+69669311 None:intergenic
TTTACAACGATGCAATGAAC+TGG 0.483997 1_1:-69670217 Msa0039730:CDS
AGTTCATTGCATCGTTGTAA+AGG 0.484172 1_1:+69670219 None:intergenic
AAAATAATTTGTTTGGTGGC+CGG 0.487549 1_1:+69670073 None:intergenic
AAGACTTTCCTAATTATTGG+TGG 0.493076 1_1:+69670267 None:intergenic
TGGTTGAGACTATTAGATAA+GGG 0.494385 1_1:+69670189 None:intergenic
ATTGAAATCAGTACTGGTAC+TGG 0.499677 1_1:-69669629 Msa0039730:CDS
TACTGGTACTGGTCTTATAG+AGG 0.512697 1_1:-69669618 Msa0039730:CDS
CTGGAACAACTGATGAAAGA+TGG 0.513185 1_1:-69669572 Msa0039730:CDS
GTTCCAGAAATTTAGAAAGT+GGG 0.518377 1_1:+69669588 None:intergenic
TTTATCTGATCAAATGAATG+AGG 0.525114 1_1:-69669801 Msa0039730:CDS
TGTTCCAGAAATTTAGAAAG+TGG 0.525663 1_1:+69669587 None:intergenic
CACAAGAATAATAGAACTGT+GGG 0.526811 1_1:-69669653 Msa0039730:CDS
GGAAGAACTAAGTTACAAAA+GGG 0.531740 1_1:-69669744 Msa0039730:CDS
GAAGCAAAGGAGACAAGAAA+TGG 0.533586 1_1:-69669974 Msa0039730:CDS
TCTCCCTCTTCACTTCATCA+AGG 0.533778 1_1:-69669923 Msa0039730:intron
TCACCTTGATGAAGTGAAGA+GGG 0.538188 1_1:+69669920 None:intergenic
GATGAAGTGAAGAGGGAGAA+GGG 0.546212 1_1:+69669927 None:intergenic
GTGGGAATTGAAATCAGTAC+TGG 0.547773 1_1:-69669635 Msa0039730:CDS
CCCACAACATCACAAAATCT+CGG 0.553306 1_1:-69670163 Msa0039730:CDS
TTCCAGAAATTTAGAAAGTG+GGG 0.570258 1_1:+69669589 None:intergenic
ATAGGGAAATTGAGAAGCAA+AGG 0.575199 1_1:-69669987 Msa0039730:CDS
TGATGAAGTGAAGAGGGAGA+AGG 0.577558 1_1:+69669926 None:intergenic
CTCAGTTAGATCTACACTGT+CGG 0.580625 1_1:+69669361 None:intergenic
GAAACCAAAGAGAGCCCACA+TGG 0.590718 1_1:+69669171 None:intergenic
ATGTAAATATTGAATCCATG+TGG 0.601602 1_1:-69669186 Msa0039730:three_prime_UTR
TTCACCTTGATGAAGTGAAG+AGG 0.603402 1_1:+69669919 None:intergenic
TGTAAATATTGAATCCATGT+GGG 0.608389 1_1:-69669185 Msa0039730:three_prime_UTR
CCACAAGAATAATAGAACTG+TGG 0.617517 1_1:-69669654 Msa0039730:CDS
GGTACTGGTCTTATAGAGGA+AGG 0.626096 1_1:-69669614 Msa0039730:CDS
TAGTGAAACTACAAGAAACA+TGG 0.661078 1_1:+69669678 None:intergenic
TTAGTTGTTCGGATTAAACA+CGG 0.664058 1_1:-69669304 Msa0039730:exon
ACTACATAGTGTGCAGTGTG+AGG 0.676728 1_1:-69669504 Msa0039730:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTATATAAAATAATTTGTT+TGG + chr1_1:69669270-69669289 None:intergenic 10.0%
!! AAATTCAAAATATTAATTAG+AGG + chr1_1:69669909-69669928 None:intergenic 10.0%
!!! TCTATATAAAATAATTTGTT+TGG + chr1_1:69669270-69669289 None:intergenic 10.0%
!! AAATTCAAAATATTAATTAG+AGG + chr1_1:69669909-69669928 None:intergenic 10.0%
!!! TGATTTTTCTCTTAAAAAAA+TGG - chr1_1:69669230-69669249 Msa0039730:three_prime_UTR 15.0%
!!! ATATAAAATAATTTGTTTGG+TGG + chr1_1:69669267-69669286 None:intergenic 15.0%
!!! TTTAATCATTAATGTTTTCT+AGG - chr1_1:69669452-69669471 Msa0039730:intron 15.0%
!!! TGATTTTTCTCTTAAAAAAA+TGG - chr1_1:69669230-69669249 Msa0039730:three_prime_UTR 15.0%
!!! ATATAAAATAATTTGTTTGG+TGG + chr1_1:69669267-69669286 None:intergenic 15.0%
!!! TTTAATCATTAATGTTTTCT+AGG - chr1_1:69669452-69669471 Msa0039730:intron 15.0%
!! TTAAAAAAATGGATACTAAC+CGG - chr1_1:69669241-69669260 Msa0039730:three_prime_UTR 20.0%
!! TATTGAAGATAAGAAGAAAA+TGG - chr1_1:69669320-69669339 Msa0039730:CDS 20.0%
!! TTATCATCTGTTATAACTTT+AGG - chr1_1:69669475-69669494 Msa0039730:intron 20.0%
!! TATCATCTGTTATAACTTTA+GGG - chr1_1:69669476-69669495 Msa0039730:intron 20.0%
!!! TTATTTATTGTTTCTCTAAC+AGG - chr1_1:69669499-69669518 Msa0039730:intron 20.0%
!!! TATTTATTGTTTCTCTAACA+GGG - chr1_1:69669500-69669519 Msa0039730:intron 20.0%
!!! AAACATGGCTTTTATTTTTA+AGG + chr1_1:69669643-69669662 None:intergenic 20.0%
!!! TATGACAATTTTTTGTAAAC+AGG + chr1_1:69670066-69670085 None:intergenic 20.0%
!! CAAAATTAATTGTGAAGTTT+AGG - chr1_1:69670249-69670268 Msa0039730:five_prime_UTR 20.0%
!! TTAAAAAAATGGATACTAAC+CGG - chr1_1:69669241-69669260 Msa0039730:three_prime_UTR 20.0%
!! TATTGAAGATAAGAAGAAAA+TGG - chr1_1:69669320-69669339 Msa0039730:CDS 20.0%
!! TTATCATCTGTTATAACTTT+AGG - chr1_1:69669475-69669494 Msa0039730:intron 20.0%
!! TATCATCTGTTATAACTTTA+GGG - chr1_1:69669476-69669495 Msa0039730:intron 20.0%
!!! TTATTTATTGTTTCTCTAAC+AGG - chr1_1:69669499-69669518 Msa0039730:intron 20.0%
!!! TATTTATTGTTTCTCTAACA+GGG - chr1_1:69669500-69669519 Msa0039730:intron 20.0%
!!! AAACATGGCTTTTATTTTTA+AGG + chr1_1:69669643-69669662 None:intergenic 20.0%
!!! TATGACAATTTTTTGTAAAC+AGG + chr1_1:69670066-69670085 None:intergenic 20.0%
!! CAAAATTAATTGTGAAGTTT+AGG - chr1_1:69670249-69670268 Msa0039730:five_prime_UTR 20.0%
! TCAAAGACTTTCCTAATTAT+TGG + chr1_1:69669072-69669091 None:intergenic 25.0%
! TATATAGATCGTACAAATCA+TGG - chr1_1:69669282-69669301 Msa0039730:three_prime_UTR 25.0%
! ATAAGAAGAAAATGGTTCAT+AGG - chr1_1:69669328-69669347 Msa0039730:CDS 25.0%
! TAAGAAGAAAATGGTTCATA+GGG - chr1_1:69669329-69669348 Msa0039730:CDS 25.0%
! TTTATCTGATCAAATGAATG+AGG - chr1_1:69669532-69669551 Msa0039730:CDS 25.0%
! AAATCACTTGAAGAAGAATA+TGG - chr1_1:69669568-69669587 Msa0039730:CDS 25.0%
!!! ACTAAATGATGGATTCATAT+TGG + chr1_1:69670014-69670033 None:intergenic 25.0%
! TTATATTCTCTCTTTCTAAC+TGG - chr1_1:69670087-69670106 Msa0039730:CDS 25.0%
! ATGTAAATATTGAATCCATG+TGG - chr1_1:69670147-69670166 Msa0039730:CDS 25.0%
! TGTAAATATTGAATCCATGT+GGG - chr1_1:69670148-69670167 Msa0039730:CDS 25.0%
! TCAAAGACTTTCCTAATTAT+TGG + chr1_1:69669072-69669091 None:intergenic 25.0%
! TATATAGATCGTACAAATCA+TGG - chr1_1:69669282-69669301 Msa0039730:three_prime_UTR 25.0%
! ATAAGAAGAAAATGGTTCAT+AGG - chr1_1:69669328-69669347 Msa0039730:CDS 25.0%
! TAAGAAGAAAATGGTTCATA+GGG - chr1_1:69669329-69669348 Msa0039730:CDS 25.0%
! TTTATCTGATCAAATGAATG+AGG - chr1_1:69669532-69669551 Msa0039730:CDS 25.0%
! AAATCACTTGAAGAAGAATA+TGG - chr1_1:69669568-69669587 Msa0039730:CDS 25.0%
!!! ACTAAATGATGGATTCATAT+TGG + chr1_1:69670014-69670033 None:intergenic 25.0%
! TTATATTCTCTCTTTCTAAC+TGG - chr1_1:69670087-69670106 Msa0039730:CDS 25.0%
! ATGTAAATATTGAATCCATG+TGG - chr1_1:69670147-69670166 Msa0039730:CDS 25.0%
! TGTAAATATTGAATCCATGT+GGG - chr1_1:69670148-69670167 Msa0039730:CDS 25.0%
AAGACTTTCCTAATTATTGG+TGG + chr1_1:69669069-69669088 None:intergenic 30.0%
TAAAGGAAACATGTTTGGTT+TGG + chr1_1:69669100-69669119 None:intergenic 30.0%
TGGTTGAGACTATTAGATAA+GGG + chr1_1:69669147-69669166 None:intergenic 30.0%
!! AAAATAATTTGTTTGGTGGC+CGG + chr1_1:69669263-69669282 None:intergenic 30.0%
TGGAAGAACTAAGTTACAAA+AGG - chr1_1:69669588-69669607 Msa0039730:CDS 30.0%
GGAAGAACTAAGTTACAAAA+GGG - chr1_1:69669589-69669608 Msa0039730:CDS 30.0%
!!! TGGCTTTTATTTTTAAGGCT+AGG + chr1_1:69669638-69669657 None:intergenic 30.0%
TAGTGAAACTACAAGAAACA+TGG + chr1_1:69669658-69669677 None:intergenic 30.0%
CACAAGAATAATAGAACTGT+GGG - chr1_1:69669680-69669699 Msa0039730:CDS 30.0%
TTCCAGAAATTTAGAAAGTG+GGG + chr1_1:69669747-69669766 None:intergenic 30.0%
GTTCCAGAAATTTAGAAAGT+GGG + chr1_1:69669748-69669767 None:intergenic 30.0%
TGTTCCAGAAATTTAGAAAG+TGG + chr1_1:69669749-69669768 None:intergenic 30.0%
! TGCTTTTCTATTCAAGTCAA+TGG - chr1_1:69669800-69669819 Msa0039730:CDS 30.0%
!!! GCACATGTTTTGACAATTTA+AGG + chr1_1:69669884-69669903 None:intergenic 30.0%
GAATCCATCATTTAGTTGTT+CGG - chr1_1:69670018-69670037 Msa0039730:CDS 30.0%
TAATCCGAACAACTAAATGA+TGG + chr1_1:69670025-69670044 None:intergenic 30.0%
TTAGTTGTTCGGATTAAACA+CGG - chr1_1:69670029-69670048 Msa0039730:CDS 30.0%
AAGACTTTCCTAATTATTGG+TGG + chr1_1:69669069-69669088 None:intergenic 30.0%
TAAAGGAAACATGTTTGGTT+TGG + chr1_1:69669100-69669119 None:intergenic 30.0%
TGGTTGAGACTATTAGATAA+GGG + chr1_1:69669147-69669166 None:intergenic 30.0%
!! AAAATAATTTGTTTGGTGGC+CGG + chr1_1:69669263-69669282 None:intergenic 30.0%
TGGAAGAACTAAGTTACAAA+AGG - chr1_1:69669588-69669607 Msa0039730:CDS 30.0%
GGAAGAACTAAGTTACAAAA+GGG - chr1_1:69669589-69669608 Msa0039730:CDS 30.0%
!!! TGGCTTTTATTTTTAAGGCT+AGG + chr1_1:69669638-69669657 None:intergenic 30.0%
TAGTGAAACTACAAGAAACA+TGG + chr1_1:69669658-69669677 None:intergenic 30.0%
CACAAGAATAATAGAACTGT+GGG - chr1_1:69669680-69669699 Msa0039730:CDS 30.0%
TTCCAGAAATTTAGAAAGTG+GGG + chr1_1:69669747-69669766 None:intergenic 30.0%
GTTCCAGAAATTTAGAAAGT+GGG + chr1_1:69669748-69669767 None:intergenic 30.0%
TGTTCCAGAAATTTAGAAAG+TGG + chr1_1:69669749-69669768 None:intergenic 30.0%
! TGCTTTTCTATTCAAGTCAA+TGG - chr1_1:69669800-69669819 Msa0039730:CDS 30.0%
!!! GCACATGTTTTGACAATTTA+AGG + chr1_1:69669884-69669903 None:intergenic 30.0%
GAATCCATCATTTAGTTGTT+CGG - chr1_1:69670018-69670037 Msa0039730:CDS 30.0%
TAATCCGAACAACTAAATGA+TGG + chr1_1:69670025-69670044 None:intergenic 30.0%
TTAGTTGTTCGGATTAAACA+CGG - chr1_1:69670029-69670048 Msa0039730:CDS 30.0%
TTCCTTAGCCACCAATAATT+AGG - chr1_1:69669058-69669077 Msa0039730:three_prime_UTR 35.0%
! TTCCTAATTATTGGTGGCTA+AGG + chr1_1:69669063-69669082 None:intergenic 35.0%
CGTTGTAAAGGAAACATGTT+TGG + chr1_1:69669105-69669124 None:intergenic 35.0%
AGTTCATTGCATCGTTGTAA+AGG + chr1_1:69669117-69669136 None:intergenic 35.0%
TTTACAACGATGCAATGAAC+TGG - chr1_1:69669116-69669135 Msa0039730:three_prime_UTR 35.0%
GTGGTTGAGACTATTAGATA+AGG + chr1_1:69669148-69669167 None:intergenic 35.0%
ATAGGGAAATTGAGAAGCAA+AGG - chr1_1:69669346-69669365 Msa0039730:CDS 35.0%
CCACAGTTCTATTATTCTTG+TGG + chr1_1:69669682-69669701 None:intergenic 35.0%
CCACAAGAATAATAGAACTG+TGG - chr1_1:69669679-69669698 Msa0039730:CDS 35.0%
!! ATTGAAATCAGTACTGGTAC+TGG - chr1_1:69669704-69669723 Msa0039730:CDS 35.0%
! TATTGAGCTTGATGATTCAC+AGG - chr1_1:69669940-69669959 Msa0039730:CDS 35.0%
TAGATCTAACTGAGCTAAGA+AGG - chr1_1:69669981-69670000 Msa0039730:CDS 35.0%
TTCCTTAGCCACCAATAATT+AGG - chr1_1:69669058-69669077 Msa0039730:three_prime_UTR 35.0%
! TTCCTAATTATTGGTGGCTA+AGG + chr1_1:69669063-69669082 None:intergenic 35.0%
CGTTGTAAAGGAAACATGTT+TGG + chr1_1:69669105-69669124 None:intergenic 35.0%
AGTTCATTGCATCGTTGTAA+AGG + chr1_1:69669117-69669136 None:intergenic 35.0%
TTTACAACGATGCAATGAAC+TGG - chr1_1:69669116-69669135 Msa0039730:three_prime_UTR 35.0%
GTGGTTGAGACTATTAGATA+AGG + chr1_1:69669148-69669167 None:intergenic 35.0%
ATAGGGAAATTGAGAAGCAA+AGG - chr1_1:69669346-69669365 Msa0039730:CDS 35.0%
CCACAGTTCTATTATTCTTG+TGG + chr1_1:69669682-69669701 None:intergenic 35.0%
CCACAAGAATAATAGAACTG+TGG - chr1_1:69669679-69669698 Msa0039730:CDS 35.0%
!! ATTGAAATCAGTACTGGTAC+TGG - chr1_1:69669704-69669723 Msa0039730:CDS 35.0%
! TATTGAGCTTGATGATTCAC+AGG - chr1_1:69669940-69669959 Msa0039730:CDS 35.0%
TAGATCTAACTGAGCTAAGA+AGG - chr1_1:69669981-69670000 Msa0039730:CDS 35.0%
! AGATTTTGTGATGTTGTGGG+TGG + chr1_1:69669170-69669189 None:intergenic 40.0%
! CCGAGATTTTGTGATGTTGT+GGG + chr1_1:69669173-69669192 None:intergenic 40.0%
CCCACAACATCACAAAATCT+CGG - chr1_1:69669170-69669189 Msa0039730:three_prime_UTR 40.0%
! TCCGAGATTTTGTGATGTTG+TGG + chr1_1:69669174-69669193 None:intergenic 40.0%
GAAGCAAAGGAGACAAGAAA+TGG - chr1_1:69669359-69669378 Msa0039730:CDS 40.0%
TCACCTTGATGAAGTGAAGA+GGG + chr1_1:69669416-69669435 None:intergenic 40.0%
TTCACCTTGATGAAGTGAAG+AGG + chr1_1:69669417-69669436 None:intergenic 40.0%
GTGGGAATTGAAATCAGTAC+TGG - chr1_1:69669698-69669717 Msa0039730:CDS 40.0%
!! TACTGGTACTGGTCTTATAG+AGG - chr1_1:69669715-69669734 Msa0039730:CDS 40.0%
TGCCCCACTTTCTAAATTTC+TGG - chr1_1:69669742-69669761 Msa0039730:CDS 40.0%
CTGGAACAACTGATGAAAGA+TGG - chr1_1:69669761-69669780 Msa0039730:CDS 40.0%
CTCAGTTAGATCTACACTGT+CGG + chr1_1:69669975-69669994 None:intergenic 40.0%
! TTTGGTTTCTGCTTCTGTCT+TGG - chr1_1:69670176-69670195 Msa0039730:CDS 40.0%
TTGGTTTCTGCTTCTGTCTT+GGG - chr1_1:69670177-69670196 Msa0039730:CDS 40.0%
! AGATTTTGTGATGTTGTGGG+TGG + chr1_1:69669170-69669189 None:intergenic 40.0%
! CCGAGATTTTGTGATGTTGT+GGG + chr1_1:69669173-69669192 None:intergenic 40.0%
CCCACAACATCACAAAATCT+CGG - chr1_1:69669170-69669189 Msa0039730:three_prime_UTR 40.0%
! TCCGAGATTTTGTGATGTTG+TGG + chr1_1:69669174-69669193 None:intergenic 40.0%
GAAGCAAAGGAGACAAGAAA+TGG - chr1_1:69669359-69669378 Msa0039730:CDS 40.0%
TCACCTTGATGAAGTGAAGA+GGG + chr1_1:69669416-69669435 None:intergenic 40.0%
TTCACCTTGATGAAGTGAAG+AGG + chr1_1:69669417-69669436 None:intergenic 40.0%
GTGGGAATTGAAATCAGTAC+TGG - chr1_1:69669698-69669717 Msa0039730:CDS 40.0%
!! TACTGGTACTGGTCTTATAG+AGG - chr1_1:69669715-69669734 Msa0039730:CDS 40.0%
TGCCCCACTTTCTAAATTTC+TGG - chr1_1:69669742-69669761 Msa0039730:CDS 40.0%
CTGGAACAACTGATGAAAGA+TGG - chr1_1:69669761-69669780 Msa0039730:CDS 40.0%
CTCAGTTAGATCTACACTGT+CGG + chr1_1:69669975-69669994 None:intergenic 40.0%
! TTTGGTTTCTGCTTCTGTCT+TGG - chr1_1:69670176-69670195 Msa0039730:CDS 40.0%
TTGGTTTCTGCTTCTGTCTT+GGG - chr1_1:69670177-69670196 Msa0039730:CDS 40.0%
! TTTTGTGATGTTGTGGGTGG+TGG + chr1_1:69669167-69669186 None:intergenic 45.0%
AAGAGGGAGAAGGGATCTAA+GGG + chr1_1:69669400-69669419 None:intergenic 45.0%
GATGAAGTGAAGAGGGAGAA+GGG + chr1_1:69669409-69669428 None:intergenic 45.0%
TGATGAAGTGAAGAGGGAGA+AGG + chr1_1:69669410-69669429 None:intergenic 45.0%
TCTCCCTCTTCACTTCATCA+AGG - chr1_1:69669410-69669429 Msa0039730:intron 45.0%
!! GGTACTGGTCTTATAGAGGA+AGG - chr1_1:69669719-69669738 Msa0039730:CDS 45.0%
ACTACATAGTGTGCAGTGTG+AGG - chr1_1:69669829-69669848 Msa0039730:intron 45.0%
! TTTTGTGATGTTGTGGGTGG+TGG + chr1_1:69669167-69669186 None:intergenic 45.0%
AAGAGGGAGAAGGGATCTAA+GGG + chr1_1:69669400-69669419 None:intergenic 45.0%
GATGAAGTGAAGAGGGAGAA+GGG + chr1_1:69669409-69669428 None:intergenic 45.0%
TGATGAAGTGAAGAGGGAGA+AGG + chr1_1:69669410-69669429 None:intergenic 45.0%
TCTCCCTCTTCACTTCATCA+AGG - chr1_1:69669410-69669429 Msa0039730:intron 45.0%
!! GGTACTGGTCTTATAGAGGA+AGG - chr1_1:69669719-69669738 Msa0039730:CDS 45.0%
ACTACATAGTGTGCAGTGTG+AGG - chr1_1:69669829-69669848 Msa0039730:intron 45.0%
GAAGAGGGAGAAGGGATCTA+AGG + chr1_1:69669401-69669420 None:intergenic 50.0%
GAATCCATGTGGGCTCTCTT+TGG - chr1_1:69670158-69670177 Msa0039730:CDS 50.0%
GAAACCAAAGAGAGCCCACA+TGG + chr1_1:69670165-69670184 None:intergenic 50.0%
GAAGAGGGAGAAGGGATCTA+AGG + chr1_1:69669401-69669420 None:intergenic 50.0%
GAATCCATGTGGGCTCTCTT+TGG - chr1_1:69670158-69670177 Msa0039730:CDS 50.0%
GAAACCAAAGAGAGCCCACA+TGG + chr1_1:69670165-69670184 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 69669040 69670315 69669040 ID=Msa0039730;Name=Msa0039730
chr1_1 mRNA 69669040 69670315 69669040 ID=Msa0039730-mRNA-1;Parent=Msa0039730;Name=Msa0039730-mRNA-1;_AED=0.05;_eAED=0.05;_QI=68|1|1|1|1|1|3|270|245
chr1_1 exon 69669040 69669393 69669040 ID=Msa0039730-mRNA-1:exon:22775;Parent=Msa0039730-mRNA-1
chr1_1 exon 69669505 69669834 69669505 ID=Msa0039730-mRNA-1:exon:22774;Parent=Msa0039730-mRNA-1
chr1_1 exon 69669924 69670315 69669924 ID=Msa0039730-mRNA-1:exon:22773;Parent=Msa0039730-mRNA-1
chr1_1 five_prime_UTR 69670248 69670315 69670248 ID=Msa0039730-mRNA-1:five_prime_utr;Parent=Msa0039730-mRNA-1
chr1_1 CDS 69669924 69670247 69669924 ID=Msa0039730-mRNA-1:cds;Parent=Msa0039730-mRNA-1
chr1_1 CDS 69669505 69669834 69669505 ID=Msa0039730-mRNA-1:cds;Parent=Msa0039730-mRNA-1
chr1_1 CDS 69669310 69669393 69669310 ID=Msa0039730-mRNA-1:cds;Parent=Msa0039730-mRNA-1
chr1_1 three_prime_UTR 69669040 69669309 69669040 ID=Msa0039730-mRNA-1:three_prime_utr;Parent=Msa0039730-mRNA-1
Gene Sequence

>Msa0039730

ATGTTTCCTTTACAACGATGCAATGAACTGGCGAAACCCTTATCTAATAGTCTCAACCACCACCCACAACATCACAAAATCTCGGAAGATCTGATTCTTGATGATTGTGATTCTCTTGTAATTGATTTTTCTCTTAAAAAAATGGATACTAACCGGCCACCAAACAAATTATTTTATATAGATCGTACAAATCATGGTAGTAATCGTAATTCTATTGAAGATAAGAAGAAAATGGTTCATAGGGAAATTGAGAAGCAAAGGAGACAAGAAATGGCTACACTTCATGCTTCCCTTAGATCCCTTCTCCCTCTTCACTTCATCAAGGGAAAACGTTCTTTATCTGATCAAATGAATGAGGCAGTGAATTACATAAATCACTTGAAGAAGAATATGGAAGAACTAAGTTACAAAAGGGATGAACTGAAAAAACTCTCTAATCCTAGCCTTAAAAATAAAAGCCATGTTTCTTGTAGTTTCACTATCCACAAGAATAATAGAACTGTGGGAATTGAAATCAGTACTGGTACTGGTCTTATAGAGGAAGGTGCCCCACTTTCTAAATTTCTGGAACAACTGATGAAAGATGGACTTGATATTGTTAGCTGCTTTTCTATTCAAGTCAATGGAAGATTACTACATAGTGTGCAGTGTGAGGTTATCGATTCCGACAGTGTAGATCTAACTGAGCTAAGAAGGAATTTTTCCAATATGAATCCATCATTTAGTTGTTCGGATTAA
Protein sequence

>Msa0039730

MFPLQRCNELAKPLSNSLNHHPQHHKISEDLILDDCDSLVIDFSLKKMDTNRPPNKLFYIDRTNHGSNRNSIEDKKKMVHREIEKQRRQEMATLHASLRSLLPLHFIKGKRSLSDQMNEAVNYINHLKKNMEELSYKRDELKKLSNPSLKNKSHVSCSFTIHKNNRTVGIEISTGTGLIEEGAPLSKFLEQLMKDGLDIVSCFSIQVNGRLLHSVQCEVIDSDSVDLTELRRNFSNMNPSFSCSD*