AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0087440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0087440 MtrunA17_Chr1g0204351 95.510 245 10 1 1 245 1 244 8.55e-173 475
Msa0087440 MtrunA17_Chr6g0474671 52.193 228 99 6 21 243 16 238 3.50e-65 202
Msa0087440 MtrunA17_Chr6g0474681 40.223 179 97 3 72 244 2 176 3.37e-34 121
Msa0087440 MtrunA17_Chr1g0204361 35.714 182 101 2 61 231 4 180 3.08e-29 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0087440 AT5G51780.2 46.328 177 79 5 74 237 23 196 6.71e-38 131
Msa0087440 AT5G51780.1 46.286 175 78 5 76 237 2 173 1.70e-37 130
Msa0087440 AT4G25400.1 44.444 171 88 3 70 237 19 185 3.09e-36 127
Msa0087440 AT4G25400.2 46.405 153 75 3 70 219 19 167 1.33e-33 120
Msa0087440 AT4G25410.1 43.636 165 83 4 80 234 47 211 2.91e-33 120
Msa0087440 AT5G51790.1 40.541 185 98 5 62 234 9 193 6.92e-32 116
Msa0087440 AT5G51790.3 40.541 185 98 5 62 234 74 258 2.37e-31 116
Msa0087440 AT5G51790.4 41.765 170 87 5 62 219 9 178 2.42e-30 112
Msa0087440 AT5G51790.2 41.765 170 87 5 62 219 74 243 1.02e-29 112
Msa0087440 AT1G62975.1 32.161 199 111 3 63 237 61 259 3.14e-24 97.8
Msa0087440 AT1G12540.1 35.196 179 101 5 76 240 75 252 5.75e-22 91.7
Msa0087440 AT1G62975.2 33.149 181 97 3 63 219 61 241 7.46e-22 91.3

Find 57 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCCCACTTTCTAAATTTC+TGG 0.103688 1_2:+68385785 Msa0087440:CDS
TCAAAGACTTTCCTAATTAT+TGG 0.193329 1_2:-68385112 None:intergenic
CAAAATTAATTGTGAAGTTT+AGG 0.202471 1_2:+68386281 Msa0087440:three_prime_UTR
GAAGAGGGAGAAGGGATCTA+AGG 0.225427 1_2:-68385441 None:intergenic
ATATATATGTTGGATTGTTT+TGG 0.261553 1_2:-68385076 None:intergenic
TTGGTTTCTGCTTCTGTCTT+GGG 0.275198 1_2:+68386209 Msa0087440:three_prime_UTR
GAATCCATGTGGGCTCTCTT+TGG 0.295811 1_2:+68386190 Msa0087440:three_prime_UTR
CTGGAACAACTGATGAAATA+TGG 0.304748 1_2:+68385804 Msa0087440:CDS
TATTGAAGATAAGAAGAAAA+TGG 0.318828 1_2:+68385363 Msa0087440:CDS
CTCCTTAGCCACCAATAATT+AGG 0.319135 1_2:+68385101 Msa0087440:five_prime_UTR
TTTGGTTTCTGCTTCTGTCT+TGG 0.319200 1_2:+68386208 Msa0087440:three_prime_UTR
TTATATTCTCTCTTTCTAAC+TGG 0.333136 1_2:+68386119 Msa0087440:three_prime_UTR
TCTATATAAAATAATTTGTT+TGG 0.336326 1_2:-68385310 None:intergenic
ATAAGAAGAAAATGGTTCAT+AGG 0.350975 1_2:+68385371 Msa0087440:CDS
ACTAAATGATGGATTCATAT+TGG 0.351963 1_2:-68386043 None:intergenic
AAATCACTTGAAGAAGAATA+TGG 0.357120 1_2:+68385611 Msa0087440:CDS
CGTTGTAAAGGAAACATGTT+TGG 0.371777 1_2:-68385145 None:intergenic
TAAAGGAAACATGTTTGGTT+TGG 0.398708 1_2:-68385140 None:intergenic
TTCCTAATTATTGGTGGCTA+AGG 0.419248 1_2:-68385103 None:intergenic
TATATAGATCGTACAAATCA+TGG 0.422086 1_2:+68385325 Msa0087440:CDS
TACTAGTACTTGCTTTATAG+AGG 0.425326 1_2:+68385758 Msa0087440:CDS
GTGGTTGAGACTATTAGATA+AGG 0.433605 1_2:-68385188 None:intergenic
TGGAAGAACTAAGTTACAAA+AGG 0.434554 1_2:+68385631 Msa0087440:CDS
CCACAGTTCTATTATTCTTG+TGG 0.455790 1_2:-68385722 None:intergenic
ATATAAAATAATTTGTTTGG+TGG 0.460635 1_2:-68385307 None:intergenic
AAGAGGGAGAAGGGATCTAA+GGG 0.467872 1_2:-68385440 None:intergenic
TAATCTGAACAACTAAATGA+TGG 0.468144 1_2:-68386054 None:intergenic
TAAGAAGAAAATGGTTCATA+GGG 0.475156 1_2:+68385372 Msa0087440:CDS
TAGATCTAACTGAGCTAAGA+AGG 0.478104 1_2:+68386013 Msa0087440:CDS
TTTACAACGATGCAATGAAC+TGG 0.483997 1_2:+68385159 Msa0087440:CDS
AGTTCATTGCATCGTTGTAA+AGG 0.484172 1_2:-68385157 None:intergenic
AAAATAATTTGTTTGGTGGC+CGG 0.487549 1_2:-68385303 None:intergenic
AAGACTTTCCTAATTATTGG+TGG 0.493076 1_2:-68385109 None:intergenic
TGGTTGAGACTATTAGATAA+GGG 0.494385 1_2:-68385187 None:intergenic
GTTCCAGAAATTTAGAAAGT+GGG 0.518377 1_2:-68385788 None:intergenic
CTAAGGAGTCATATATATGT+TGG 0.521802 1_2:-68385086 None:intergenic
TTTATCTGATCAAATGAATG+AGG 0.525114 1_2:+68385575 Msa0087440:CDS
TGTTCCAGAAATTTAGAAAG+TGG 0.525663 1_2:-68385789 None:intergenic
CACAAGAATAATAGAACTGT+GGG 0.526811 1_2:+68385723 Msa0087440:CDS
GGAAGAACTAAGTTACAAAA+GGG 0.531740 1_2:+68385632 Msa0087440:CDS
GAAGCAAAGGAGACAAGAAA+TGG 0.533586 1_2:+68385402 Msa0087440:CDS
TCTCCCTCTTCACTTCATCA+AGG 0.533778 1_2:+68385453 Msa0087440:CDS
TCACCTTGATGAAGTGAAGA+GGG 0.538188 1_2:-68385456 None:intergenic
GATGAAGTGAAGAGGGAGAA+GGG 0.546212 1_2:-68385449 None:intergenic
CCCACAACATCACAAAATCT+CGG 0.553306 1_2:+68385213 Msa0087440:CDS
AGTACTTGCTTTATAGAGGA+AGG 0.558027 1_2:+68385762 Msa0087440:CDS
TTCCAGAAATTTAGAAAGTG+GGG 0.570258 1_2:-68385787 None:intergenic
ATAGGGAAATTGAGAAGCAA+AGG 0.575199 1_2:+68385389 Msa0087440:CDS
TGATGAAGTGAAGAGGGAGA+AGG 0.577558 1_2:-68385450 None:intergenic
CTCAGTTAGATCTACACTGT+CGG 0.580625 1_2:-68386004 None:intergenic
GAAACCAAAGAGAGCCCACA+TGG 0.590718 1_2:-68386194 None:intergenic
TTCACCTTGATGAAGTGAAG+AGG 0.603402 1_2:-68385457 None:intergenic
TGTAAATATTGAATCCATGT+GGG 0.608389 1_2:+68386180 Msa0087440:three_prime_UTR
CCACAAGAATAATAGAACTG+TGG 0.617517 1_2:+68385722 Msa0087440:CDS
ATGTAAATATTGAATCCATG+TGG 0.661065 1_2:+68386179 Msa0087440:three_prime_UTR
TAGTGAAACTACAAGAAACA+TGG 0.661078 1_2:-68385698 None:intergenic
ACTACATAGTGTGCAGTGTG+AGG 0.664309 1_2:+68385872 Msa0087440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTATATAAAATAATTTGTT+TGG - chr1_2:68385313-68385332 None:intergenic 10.0%
!!! TCTATATAAAATAATTTGTT+TGG - chr1_2:68385313-68385332 None:intergenic 10.0%
!!! TGATTTTTCTCTTAAAAAAA+TGG + chr1_2:68385273-68385292 Msa0087440:CDS 15.0%
!!! ATATAAAATAATTTGTTTGG+TGG - chr1_2:68385310-68385329 None:intergenic 15.0%
!!! TTTAATCATTAAAGTTTTCT+AGG + chr1_2:68385495-68385514 Msa0087440:intron 15.0%
!!! TGATTTTTCTCTTAAAAAAA+TGG + chr1_2:68385273-68385292 Msa0087440:CDS 15.0%
!!! ATATAAAATAATTTGTTTGG+TGG - chr1_2:68385310-68385329 None:intergenic 15.0%
!!! TTTAATCATTAAAGTTTTCT+AGG + chr1_2:68385495-68385514 Msa0087440:intron 15.0%
!! TTAAAAAAATGGATACTAAC+CGG + chr1_2:68385284-68385303 Msa0087440:CDS 20.0%
!! TATTGAAGATAAGAAGAAAA+TGG + chr1_2:68385363-68385382 Msa0087440:CDS 20.0%
!! TTATCATCTGTTATAACTTT+AGG + chr1_2:68385518-68385537 Msa0087440:intron 20.0%
!! TATCATCTGTTATAACTTTA+GGG + chr1_2:68385519-68385538 Msa0087440:intron 20.0%
!!! TTATTTATTGTTTCTCAAAC+AGG + chr1_2:68385542-68385561 Msa0087440:intron 20.0%
!!! TATTTATTGTTTCTCAAACA+GGG + chr1_2:68385543-68385562 Msa0087440:intron 20.0%
!!! AAACATGGCTTTTATTTTTA+AGG - chr1_2:68385686-68385705 None:intergenic 20.0%
!!! TATGACAATTTTTTGTAAAC+AGG - chr1_2:68386098-68386117 None:intergenic 20.0%
!! CAAAATTAATTGTGAAGTTT+AGG + chr1_2:68386281-68386300 Msa0087440:three_prime_UTR 20.0%
!! TTAAAAAAATGGATACTAAC+CGG + chr1_2:68385284-68385303 Msa0087440:CDS 20.0%
!! TATTGAAGATAAGAAGAAAA+TGG + chr1_2:68385363-68385382 Msa0087440:CDS 20.0%
!! TTATCATCTGTTATAACTTT+AGG + chr1_2:68385518-68385537 Msa0087440:intron 20.0%
!! TATCATCTGTTATAACTTTA+GGG + chr1_2:68385519-68385538 Msa0087440:intron 20.0%
!!! TTATTTATTGTTTCTCAAAC+AGG + chr1_2:68385542-68385561 Msa0087440:intron 20.0%
!!! TATTTATTGTTTCTCAAACA+GGG + chr1_2:68385543-68385562 Msa0087440:intron 20.0%
!!! AAACATGGCTTTTATTTTTA+AGG - chr1_2:68385686-68385705 None:intergenic 20.0%
!!! TATGACAATTTTTTGTAAAC+AGG - chr1_2:68386098-68386117 None:intergenic 20.0%
!! CAAAATTAATTGTGAAGTTT+AGG + chr1_2:68386281-68386300 Msa0087440:three_prime_UTR 20.0%
! TCAAAGACTTTCCTAATTAT+TGG - chr1_2:68385115-68385134 None:intergenic 25.0%
! TATATAGATCGTACAAATCA+TGG + chr1_2:68385325-68385344 Msa0087440:CDS 25.0%
! ATAAGAAGAAAATGGTTCAT+AGG + chr1_2:68385371-68385390 Msa0087440:CDS 25.0%
! TAAGAAGAAAATGGTTCATA+GGG + chr1_2:68385372-68385391 Msa0087440:CDS 25.0%
! TTTATCTGATCAAATGAATG+AGG + chr1_2:68385575-68385594 Msa0087440:CDS 25.0%
! AAATCACTTGAAGAAGAATA+TGG + chr1_2:68385611-68385630 Msa0087440:CDS 25.0%
!!! ACTAAATGATGGATTCATAT+TGG - chr1_2:68386046-68386065 None:intergenic 25.0%
! TAATCTGAACAACTAAATGA+TGG - chr1_2:68386057-68386076 None:intergenic 25.0%
! TTATATTCTCTCTTTCTAAC+TGG + chr1_2:68386119-68386138 Msa0087440:three_prime_UTR 25.0%
! ATGTAAATATTGAATCCATG+TGG + chr1_2:68386179-68386198 Msa0087440:three_prime_UTR 25.0%
! TGTAAATATTGAATCCATGT+GGG + chr1_2:68386180-68386199 Msa0087440:three_prime_UTR 25.0%
! TCAAAGACTTTCCTAATTAT+TGG - chr1_2:68385115-68385134 None:intergenic 25.0%
! TATATAGATCGTACAAATCA+TGG + chr1_2:68385325-68385344 Msa0087440:CDS 25.0%
! ATAAGAAGAAAATGGTTCAT+AGG + chr1_2:68385371-68385390 Msa0087440:CDS 25.0%
! TAAGAAGAAAATGGTTCATA+GGG + chr1_2:68385372-68385391 Msa0087440:CDS 25.0%
! TTTATCTGATCAAATGAATG+AGG + chr1_2:68385575-68385594 Msa0087440:CDS 25.0%
! AAATCACTTGAAGAAGAATA+TGG + chr1_2:68385611-68385630 Msa0087440:CDS 25.0%
!!! ACTAAATGATGGATTCATAT+TGG - chr1_2:68386046-68386065 None:intergenic 25.0%
! TAATCTGAACAACTAAATGA+TGG - chr1_2:68386057-68386076 None:intergenic 25.0%
! TTATATTCTCTCTTTCTAAC+TGG + chr1_2:68386119-68386138 Msa0087440:three_prime_UTR 25.0%
! ATGTAAATATTGAATCCATG+TGG + chr1_2:68386179-68386198 Msa0087440:three_prime_UTR 25.0%
! TGTAAATATTGAATCCATGT+GGG + chr1_2:68386180-68386199 Msa0087440:three_prime_UTR 25.0%
CTAAGGAGTCATATATATGT+TGG - chr1_2:68385089-68385108 None:intergenic 30.0%
AAGACTTTCCTAATTATTGG+TGG - chr1_2:68385112-68385131 None:intergenic 30.0%
TAAAGGAAACATGTTTGGTT+TGG - chr1_2:68385143-68385162 None:intergenic 30.0%
TGGTTGAGACTATTAGATAA+GGG - chr1_2:68385190-68385209 None:intergenic 30.0%
!! AAAATAATTTGTTTGGTGGC+CGG - chr1_2:68385306-68385325 None:intergenic 30.0%
TGGAAGAACTAAGTTACAAA+AGG + chr1_2:68385631-68385650 Msa0087440:CDS 30.0%
GGAAGAACTAAGTTACAAAA+GGG + chr1_2:68385632-68385651 Msa0087440:CDS 30.0%
!!! TGGCTTTTATTTTTAAGGCT+AGG - chr1_2:68385681-68385700 None:intergenic 30.0%
TAGTGAAACTACAAGAAACA+TGG - chr1_2:68385701-68385720 None:intergenic 30.0%
CACAAGAATAATAGAACTGT+GGG + chr1_2:68385723-68385742 Msa0087440:CDS 30.0%
TACTAGTACTTGCTTTATAG+AGG + chr1_2:68385758-68385777 Msa0087440:CDS 30.0%
TTCCAGAAATTTAGAAAGTG+GGG - chr1_2:68385790-68385809 None:intergenic 30.0%
GTTCCAGAAATTTAGAAAGT+GGG - chr1_2:68385791-68385810 None:intergenic 30.0%
TGTTCCAGAAATTTAGAAAG+TGG - chr1_2:68385792-68385811 None:intergenic 30.0%
! TGCTTTTCTATTCAAGTCAA+TGG + chr1_2:68385843-68385862 Msa0087440:CDS 30.0%
CTAAGGAGTCATATATATGT+TGG - chr1_2:68385089-68385108 None:intergenic 30.0%
AAGACTTTCCTAATTATTGG+TGG - chr1_2:68385112-68385131 None:intergenic 30.0%
TAAAGGAAACATGTTTGGTT+TGG - chr1_2:68385143-68385162 None:intergenic 30.0%
TGGTTGAGACTATTAGATAA+GGG - chr1_2:68385190-68385209 None:intergenic 30.0%
!! AAAATAATTTGTTTGGTGGC+CGG - chr1_2:68385306-68385325 None:intergenic 30.0%
TGGAAGAACTAAGTTACAAA+AGG + chr1_2:68385631-68385650 Msa0087440:CDS 30.0%
GGAAGAACTAAGTTACAAAA+GGG + chr1_2:68385632-68385651 Msa0087440:CDS 30.0%
!!! TGGCTTTTATTTTTAAGGCT+AGG - chr1_2:68385681-68385700 None:intergenic 30.0%
TAGTGAAACTACAAGAAACA+TGG - chr1_2:68385701-68385720 None:intergenic 30.0%
CACAAGAATAATAGAACTGT+GGG + chr1_2:68385723-68385742 Msa0087440:CDS 30.0%
TACTAGTACTTGCTTTATAG+AGG + chr1_2:68385758-68385777 Msa0087440:CDS 30.0%
TTCCAGAAATTTAGAAAGTG+GGG - chr1_2:68385790-68385809 None:intergenic 30.0%
GTTCCAGAAATTTAGAAAGT+GGG - chr1_2:68385791-68385810 None:intergenic 30.0%
TGTTCCAGAAATTTAGAAAG+TGG - chr1_2:68385792-68385811 None:intergenic 30.0%
! TGCTTTTCTATTCAAGTCAA+TGG + chr1_2:68385843-68385862 Msa0087440:CDS 30.0%
! TTCCTAATTATTGGTGGCTA+AGG - chr1_2:68385106-68385125 None:intergenic 35.0%
CGTTGTAAAGGAAACATGTT+TGG - chr1_2:68385148-68385167 None:intergenic 35.0%
AGTTCATTGCATCGTTGTAA+AGG - chr1_2:68385160-68385179 None:intergenic 35.0%
TTTACAACGATGCAATGAAC+TGG + chr1_2:68385159-68385178 Msa0087440:CDS 35.0%
GTGGTTGAGACTATTAGATA+AGG - chr1_2:68385191-68385210 None:intergenic 35.0%
ATAGGGAAATTGAGAAGCAA+AGG + chr1_2:68385389-68385408 Msa0087440:CDS 35.0%
CCACAGTTCTATTATTCTTG+TGG - chr1_2:68385725-68385744 None:intergenic 35.0%
CCACAAGAATAATAGAACTG+TGG + chr1_2:68385722-68385741 Msa0087440:CDS 35.0%
AGTACTTGCTTTATAGAGGA+AGG + chr1_2:68385762-68385781 Msa0087440:CDS 35.0%
CTGGAACAACTGATGAAATA+TGG + chr1_2:68385804-68385823 Msa0087440:CDS 35.0%
! TATTGAGCTTGATGATTCAC+AGG + chr1_2:68385972-68385991 Msa0087440:intron 35.0%
TAGATCTAACTGAGCTAAGA+AGG + chr1_2:68386013-68386032 Msa0087440:CDS 35.0%
! TTCCTAATTATTGGTGGCTA+AGG - chr1_2:68385106-68385125 None:intergenic 35.0%
CGTTGTAAAGGAAACATGTT+TGG - chr1_2:68385148-68385167 None:intergenic 35.0%
AGTTCATTGCATCGTTGTAA+AGG - chr1_2:68385160-68385179 None:intergenic 35.0%
TTTACAACGATGCAATGAAC+TGG + chr1_2:68385159-68385178 Msa0087440:CDS 35.0%
GTGGTTGAGACTATTAGATA+AGG - chr1_2:68385191-68385210 None:intergenic 35.0%
ATAGGGAAATTGAGAAGCAA+AGG + chr1_2:68385389-68385408 Msa0087440:CDS 35.0%
CCACAGTTCTATTATTCTTG+TGG - chr1_2:68385725-68385744 None:intergenic 35.0%
CCACAAGAATAATAGAACTG+TGG + chr1_2:68385722-68385741 Msa0087440:CDS 35.0%
AGTACTTGCTTTATAGAGGA+AGG + chr1_2:68385762-68385781 Msa0087440:CDS 35.0%
CTGGAACAACTGATGAAATA+TGG + chr1_2:68385804-68385823 Msa0087440:CDS 35.0%
! TATTGAGCTTGATGATTCAC+AGG + chr1_2:68385972-68385991 Msa0087440:intron 35.0%
TAGATCTAACTGAGCTAAGA+AGG + chr1_2:68386013-68386032 Msa0087440:CDS 35.0%
CTCCTTAGCCACCAATAATT+AGG + chr1_2:68385101-68385120 Msa0087440:five_prime_UTR 40.0%
! AGATTTTGTGATGTTGTGGG+TGG - chr1_2:68385213-68385232 None:intergenic 40.0%
! CCGAGATTTTGTGATGTTGT+GGG - chr1_2:68385216-68385235 None:intergenic 40.0%
CCCACAACATCACAAAATCT+CGG + chr1_2:68385213-68385232 Msa0087440:CDS 40.0%
! TCCGAGATTTTGTGATGTTG+TGG - chr1_2:68385217-68385236 None:intergenic 40.0%
GAAGCAAAGGAGACAAGAAA+TGG + chr1_2:68385402-68385421 Msa0087440:CDS 40.0%
TCACCTTGATGAAGTGAAGA+GGG - chr1_2:68385459-68385478 None:intergenic 40.0%
TTCACCTTGATGAAGTGAAG+AGG - chr1_2:68385460-68385479 None:intergenic 40.0%
TGCCCCACTTTCTAAATTTC+TGG + chr1_2:68385785-68385804 Msa0087440:CDS 40.0%
CTCAGTTAGATCTACACTGT+CGG - chr1_2:68386007-68386026 None:intergenic 40.0%
! TTTGGTTTCTGCTTCTGTCT+TGG + chr1_2:68386208-68386227 Msa0087440:three_prime_UTR 40.0%
TTGGTTTCTGCTTCTGTCTT+GGG + chr1_2:68386209-68386228 Msa0087440:three_prime_UTR 40.0%
CTCCTTAGCCACCAATAATT+AGG + chr1_2:68385101-68385120 Msa0087440:five_prime_UTR 40.0%
! AGATTTTGTGATGTTGTGGG+TGG - chr1_2:68385213-68385232 None:intergenic 40.0%
! CCGAGATTTTGTGATGTTGT+GGG - chr1_2:68385216-68385235 None:intergenic 40.0%
CCCACAACATCACAAAATCT+CGG + chr1_2:68385213-68385232 Msa0087440:CDS 40.0%
! TCCGAGATTTTGTGATGTTG+TGG - chr1_2:68385217-68385236 None:intergenic 40.0%
GAAGCAAAGGAGACAAGAAA+TGG + chr1_2:68385402-68385421 Msa0087440:CDS 40.0%
TCACCTTGATGAAGTGAAGA+GGG - chr1_2:68385459-68385478 None:intergenic 40.0%
TTCACCTTGATGAAGTGAAG+AGG - chr1_2:68385460-68385479 None:intergenic 40.0%
TGCCCCACTTTCTAAATTTC+TGG + chr1_2:68385785-68385804 Msa0087440:CDS 40.0%
CTCAGTTAGATCTACACTGT+CGG - chr1_2:68386007-68386026 None:intergenic 40.0%
! TTTGGTTTCTGCTTCTGTCT+TGG + chr1_2:68386208-68386227 Msa0087440:three_prime_UTR 40.0%
TTGGTTTCTGCTTCTGTCTT+GGG + chr1_2:68386209-68386228 Msa0087440:three_prime_UTR 40.0%
! TTTTGTGATGTTGTGGGTGG+TGG - chr1_2:68385210-68385229 None:intergenic 45.0%
AAGAGGGAGAAGGGATCTAA+GGG - chr1_2:68385443-68385462 None:intergenic 45.0%
GATGAAGTGAAGAGGGAGAA+GGG - chr1_2:68385452-68385471 None:intergenic 45.0%
TGATGAAGTGAAGAGGGAGA+AGG - chr1_2:68385453-68385472 None:intergenic 45.0%
TCTCCCTCTTCACTTCATCA+AGG + chr1_2:68385453-68385472 Msa0087440:CDS 45.0%
ACTACATAGTGTGCAGTGTG+AGG + chr1_2:68385872-68385891 Msa0087440:CDS 45.0%
! TTTTGTGATGTTGTGGGTGG+TGG - chr1_2:68385210-68385229 None:intergenic 45.0%
AAGAGGGAGAAGGGATCTAA+GGG - chr1_2:68385443-68385462 None:intergenic 45.0%
GATGAAGTGAAGAGGGAGAA+GGG - chr1_2:68385452-68385471 None:intergenic 45.0%
TGATGAAGTGAAGAGGGAGA+AGG - chr1_2:68385453-68385472 None:intergenic 45.0%
TCTCCCTCTTCACTTCATCA+AGG + chr1_2:68385453-68385472 Msa0087440:CDS 45.0%
ACTACATAGTGTGCAGTGTG+AGG + chr1_2:68385872-68385891 Msa0087440:CDS 45.0%
GAAGAGGGAGAAGGGATCTA+AGG - chr1_2:68385444-68385463 None:intergenic 50.0%
GAATCCATGTGGGCTCTCTT+TGG + chr1_2:68386190-68386209 Msa0087440:three_prime_UTR 50.0%
GAAACCAAAGAGAGCCCACA+TGG - chr1_2:68386197-68386216 None:intergenic 50.0%
GAAGAGGGAGAAGGGATCTA+AGG - chr1_2:68385444-68385463 None:intergenic 50.0%
GAATCCATGTGGGCTCTCTT+TGG + chr1_2:68386190-68386209 Msa0087440:three_prime_UTR 50.0%
GAAACCAAAGAGAGCCCACA+TGG - chr1_2:68386197-68386216 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 68385078 68386347 68385078 ID=Msa0087440;Name=Msa0087440
chr1_2 mRNA 68385078 68386347 68385078 ID=Msa0087440-mRNA-1;Parent=Msa0087440;Name=Msa0087440-mRNA-1;_AED=0.03;_eAED=0.03;_QI=73|1|1|1|1|1|3|270|245
chr1_2 exon 68385078 68385474 68385078 ID=Msa0087440-mRNA-1:exon:23263;Parent=Msa0087440-mRNA-1
chr1_2 exon 68385564 68385893 68385564 ID=Msa0087440-mRNA-1:exon:23264;Parent=Msa0087440-mRNA-1
chr1_2 exon 68385994 68386347 68385994 ID=Msa0087440-mRNA-1:exon:23265;Parent=Msa0087440-mRNA-1
chr1_2 five_prime_UTR 68385078 68385150 68385078 ID=Msa0087440-mRNA-1:five_prime_utr;Parent=Msa0087440-mRNA-1
chr1_2 CDS 68385151 68385474 68385151 ID=Msa0087440-mRNA-1:cds;Parent=Msa0087440-mRNA-1
chr1_2 CDS 68385564 68385893 68385564 ID=Msa0087440-mRNA-1:cds;Parent=Msa0087440-mRNA-1
chr1_2 CDS 68385994 68386077 68385994 ID=Msa0087440-mRNA-1:cds;Parent=Msa0087440-mRNA-1
chr1_2 three_prime_UTR 68386078 68386347 68386078 ID=Msa0087440-mRNA-1:three_prime_utr;Parent=Msa0087440-mRNA-1
Gene Sequence

>Msa0087440

ATGTTTCCTTTACAACGATGCAATGAACTGGCGAAACCCTTATCTAATAGTCTCAACCACCACCCACAACATCACAAAATCTCGGAAGATCTGATTCTTGATGATTGTGATTCTCTTGTAATTGATTTTTCTCTTAAAAAAATGGATACTAACCGGCCACCAAACAAATTATTTTATATAGATCGTACAAATCATGGTAGTAATCGTAATTCTATTGAAGATAAGAAGAAAATGGTTCATAGGGAAATTGAGAAGCAAAGGAGACAAGAAATGGCTACACTTCATGCTTCCCTTAGATCCCTTCTCCCTCTTCACTTCATCAAGGGAAAACGTTCTTTATCTGATCAAATGAATGAGGCAGTGAATTACATAAATCACTTGAAGAAGAATATGGAAGAACTAAGTTACAAAAGGGATGAACTGAAAAAACTCTCTAATCCTAGCCTTAAAAATAAAAGCCATGTTTCTTGTAGTTTCACTATCCACAAGAATAATAGAACTGTGGGAATTGAAATCAGTACTAGTACTTGCTTTATAGAGGAAGGTGCCCCACTTTCTAAATTTCTGGAACAACTGATGAAATATGGACTTGATGTTGTTAGCTGCTTTTCTATTCAAGTCAATGGAAGATTACTACATAGTGTGCAGTGTGAGGTTATCGATTCCGACAGTGTAGATCTAACTGAGCTAAGAAGGAATTTTTCCAATATGAATCCATCATTTAGTTGTTCAGATTAA
Protein sequence

>Msa0087440

MFPLQRCNELAKPLSNSLNHHPQHHKISEDLILDDCDSLVIDFSLKKMDTNRPPNKLFYIDRTNHGSNRNSIEDKKKMVHREIEKQRRQEMATLHASLRSLLPLHFIKGKRSLSDQMNEAVNYINHLKKNMEELSYKRDELKKLSNPSLKNKSHVSCSFTIHKNNRTVGIEISTSTCFIEEGAPLSKFLEQLMKYGLDVVSCFSIQVNGRLLHSVQCEVIDSDSVDLTELRRNFSNMNPSFSCSD*