AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0180950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0180950 MtrunA17_Chr1g0204351 95.510 245 10 1 1 245 1 244 8.55e-173 475
Msa0180950 MtrunA17_Chr6g0474671 52.193 228 99 6 21 243 16 238 3.50e-65 202
Msa0180950 MtrunA17_Chr6g0474681 40.223 179 97 3 72 244 2 176 3.37e-34 121
Msa0180950 MtrunA17_Chr1g0204361 35.714 182 101 2 61 231 4 180 3.08e-29 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0180950 AT5G51780.2 46.328 177 79 5 74 237 23 196 6.71e-38 131
Msa0180950 AT5G51780.1 46.286 175 78 5 76 237 2 173 1.70e-37 130
Msa0180950 AT4G25400.1 44.444 171 88 3 70 237 19 185 3.09e-36 127
Msa0180950 AT4G25400.2 46.405 153 75 3 70 219 19 167 1.33e-33 120
Msa0180950 AT4G25410.1 43.636 165 83 4 80 234 47 211 2.91e-33 120
Msa0180950 AT5G51790.1 40.541 185 98 5 62 234 9 193 6.92e-32 116
Msa0180950 AT5G51790.3 40.541 185 98 5 62 234 74 258 2.37e-31 116
Msa0180950 AT5G51790.4 41.765 170 87 5 62 219 9 178 2.42e-30 112
Msa0180950 AT5G51790.2 41.765 170 87 5 62 219 74 243 1.02e-29 112
Msa0180950 AT1G62975.1 32.161 199 111 3 63 237 61 259 3.14e-24 97.8
Msa0180950 AT1G12540.1 35.196 179 101 5 76 240 75 252 5.75e-22 91.7
Msa0180950 AT1G62975.2 33.149 181 97 3 63 219 61 241 7.46e-22 91.3

Find 57 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCCCACTTTCTAAATTTC+TGG 0.103688 1_4:-68955092 Msa0180950:CDS
TCAAAGACTTTCCTAATTAT+TGG 0.193329 1_4:+68955765 None:intergenic
CAAAATTAATTGTGAAGTTT+AGG 0.202471 1_4:-68954596 Msa0180950:three_prime_UTR
GAAGAGGGAGAAGGGATCTA+AGG 0.225427 1_4:+68955436 None:intergenic
ATATATATGTTGGATTGTTT+TGG 0.249216 1_4:+68955801 None:intergenic
TTGGTTTCTGCTTCTGTCTT+GGG 0.275198 1_4:-68954668 Msa0180950:three_prime_UTR
TTCCTTAGCCACCAATAATT+AGG 0.289757 1_4:-68955776 Msa0180950:five_prime_UTR
GAATCCATGTGGGCTCTCTT+TGG 0.295811 1_4:-68954687 Msa0180950:three_prime_UTR
CTGGAACAACTGATGAAATA+TGG 0.304748 1_4:-68955073 Msa0180950:CDS
TATTGAAGATAAGAAGAAAA+TGG 0.318828 1_4:-68955514 Msa0180950:CDS
TTTGGTTTCTGCTTCTGTCT+TGG 0.319200 1_4:-68954669 Msa0180950:three_prime_UTR
TTATATTCTCTCTTTCTAAC+TGG 0.333136 1_4:-68954758 Msa0180950:three_prime_UTR
TCTATATAAAATAATTTGTT+TGG 0.336326 1_4:+68955567 None:intergenic
ATAAGAAGAAAATGGTTCAT+AGG 0.350975 1_4:-68955506 Msa0180950:CDS
ACTAAATGATGGATTCATAT+TGG 0.351963 1_4:+68954834 None:intergenic
AAATCACTTGAAGAAGAATA+TGG 0.357120 1_4:-68955266 Msa0180950:CDS
CGTTGTAAAGGAAACATGTT+TGG 0.371777 1_4:+68955732 None:intergenic
TAAAGGAAACATGTTTGGTT+TGG 0.398708 1_4:+68955737 None:intergenic
TTCCTAATTATTGGTGGCTA+AGG 0.406014 1_4:+68955774 None:intergenic
TATATAGATCGTACAAATCA+TGG 0.422086 1_4:-68955552 Msa0180950:CDS
TACTAGTACTTGCTTTATAG+AGG 0.425326 1_4:-68955119 Msa0180950:CDS
GTGGTTGAGACTATTAGATA+AGG 0.433605 1_4:+68955689 None:intergenic
TGGAAGAACTAAGTTACAAA+AGG 0.434554 1_4:-68955246 Msa0180950:CDS
CCACAGTTCTATTATTCTTG+TGG 0.455790 1_4:+68955155 None:intergenic
ATATAAAATAATTTGTTTGG+TGG 0.460635 1_4:+68955570 None:intergenic
AAGAGGGAGAAGGGATCTAA+GGG 0.467872 1_4:+68955437 None:intergenic
TAATCTGAACAACTAAATGA+TGG 0.468144 1_4:+68954823 None:intergenic
TAAGAAGAAAATGGTTCATA+GGG 0.475156 1_4:-68955505 Msa0180950:CDS
TAGATCTAACTGAGCTAAGA+AGG 0.478104 1_4:-68954864 Msa0180950:CDS
TTTACAACGATGCAATGAAC+TGG 0.483997 1_4:-68955718 Msa0180950:CDS
AGTTCATTGCATCGTTGTAA+AGG 0.484172 1_4:+68955720 None:intergenic
AAAATAATTTGTTTGGTGGC+CGG 0.487549 1_4:+68955574 None:intergenic
AAGACTTTCCTAATTATTGG+TGG 0.493076 1_4:+68955768 None:intergenic
TGGTTGAGACTATTAGATAA+GGG 0.494385 1_4:+68955690 None:intergenic
CTAAGGAATCATATATATGT+TGG 0.508479 1_4:+68955791 None:intergenic
GTTCCAGAAATTTAGAAAGT+GGG 0.518377 1_4:+68955089 None:intergenic
TTTATCTGATCAAATGAATG+AGG 0.525114 1_4:-68955302 Msa0180950:CDS
TGTTCCAGAAATTTAGAAAG+TGG 0.525663 1_4:+68955088 None:intergenic
CACAAGAATAATAGAACTGT+GGG 0.526811 1_4:-68955154 Msa0180950:CDS
GGAAGAACTAAGTTACAAAA+GGG 0.531740 1_4:-68955245 Msa0180950:CDS
GAAGCAAAGGAGACAAGAAA+TGG 0.533586 1_4:-68955475 Msa0180950:CDS
TCTCCCTCTTCACTTCATCA+AGG 0.533778 1_4:-68955424 Msa0180950:intron
TCACCTTGATGAAGTGAAGA+GGG 0.538188 1_4:+68955421 None:intergenic
GATGAAGTGAAGAGGGAGAA+GGG 0.546212 1_4:+68955428 None:intergenic
CCCACAACATCACAAAATCT+CGG 0.553306 1_4:-68955664 Msa0180950:CDS
AGTACTTGCTTTATAGAGGA+AGG 0.558027 1_4:-68955115 Msa0180950:CDS
TTCCAGAAATTTAGAAAGTG+GGG 0.570258 1_4:+68955090 None:intergenic
ATAGGGAAATTGAGAAGCAA+AGG 0.575199 1_4:-68955488 Msa0180950:CDS
TGATGAAGTGAAGAGGGAGA+AGG 0.577558 1_4:+68955427 None:intergenic
CTCAGTTAGATCTACACTGT+CGG 0.580625 1_4:+68954873 None:intergenic
GAAACCAAAGAGAGCCCACA+TGG 0.590718 1_4:+68954683 None:intergenic
TTCACCTTGATGAAGTGAAG+AGG 0.603402 1_4:+68955420 None:intergenic
TGTAAATATTGAATCCATGT+GGG 0.608389 1_4:-68954697 Msa0180950:three_prime_UTR
CCACAAGAATAATAGAACTG+TGG 0.617517 1_4:-68955155 Msa0180950:CDS
ATGTAAATATTGAATCCATG+TGG 0.661065 1_4:-68954698 Msa0180950:three_prime_UTR
TAGTGAAACTACAAGAAACA+TGG 0.661078 1_4:+68955179 None:intergenic
ACTACATAGTGTGCAGTGTG+AGG 0.664309 1_4:-68955005 Msa0180950:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTATATAAAATAATTTGTT+TGG + chr1_4:68954787-68954806 None:intergenic 10.0%
!!! TCTATATAAAATAATTTGTT+TGG + chr1_4:68954787-68954806 None:intergenic 10.0%
!!! TGATTTTTCTCTTAAAAAAA+TGG - chr1_4:68954747-68954766 Msa0180950:three_prime_UTR 15.0%
!!! ATATAAAATAATTTGTTTGG+TGG + chr1_4:68954784-68954803 None:intergenic 15.0%
!!! TTTAATCATTAAAGTTTTCT+AGG - chr1_4:68954969-68954988 Msa0180950:intron 15.0%
!!! TGATTTTTCTCTTAAAAAAA+TGG - chr1_4:68954747-68954766 Msa0180950:three_prime_UTR 15.0%
!!! ATATAAAATAATTTGTTTGG+TGG + chr1_4:68954784-68954803 None:intergenic 15.0%
!!! TTTAATCATTAAAGTTTTCT+AGG - chr1_4:68954969-68954988 Msa0180950:intron 15.0%
!! TTAAAAAAATGGATACTAAC+CGG - chr1_4:68954758-68954777 Msa0180950:three_prime_UTR 20.0%
!! TATTGAAGATAAGAAGAAAA+TGG - chr1_4:68954837-68954856 Msa0180950:CDS 20.0%
!! TTATCATCTGTTATAACTTT+AGG - chr1_4:68954992-68955011 Msa0180950:intron 20.0%
!! TATCATCTGTTATAACTTTA+GGG - chr1_4:68954993-68955012 Msa0180950:intron 20.0%
!!! TTATTTATTGTTTCTCTAAC+AGG - chr1_4:68955016-68955035 Msa0180950:CDS 20.0%
!!! TATTTATTGTTTCTCTAACA+GGG - chr1_4:68955017-68955036 Msa0180950:CDS 20.0%
!!! AAACATGGCTTTTATTTTTA+AGG + chr1_4:68955160-68955179 None:intergenic 20.0%
!!! TATGACAATTTTTTGTAAAC+AGG + chr1_4:68955572-68955591 None:intergenic 20.0%
!! CAAAATTAATTGTGAAGTTT+AGG - chr1_4:68955755-68955774 Msa0180950:five_prime_UTR 20.0%
!! TTAAAAAAATGGATACTAAC+CGG - chr1_4:68954758-68954777 Msa0180950:three_prime_UTR 20.0%
!! TATTGAAGATAAGAAGAAAA+TGG - chr1_4:68954837-68954856 Msa0180950:CDS 20.0%
!! TTATCATCTGTTATAACTTT+AGG - chr1_4:68954992-68955011 Msa0180950:intron 20.0%
!! TATCATCTGTTATAACTTTA+GGG - chr1_4:68954993-68955012 Msa0180950:intron 20.0%
!!! TTATTTATTGTTTCTCTAAC+AGG - chr1_4:68955016-68955035 Msa0180950:CDS 20.0%
!!! TATTTATTGTTTCTCTAACA+GGG - chr1_4:68955017-68955036 Msa0180950:CDS 20.0%
!!! AAACATGGCTTTTATTTTTA+AGG + chr1_4:68955160-68955179 None:intergenic 20.0%
!!! TATGACAATTTTTTGTAAAC+AGG + chr1_4:68955572-68955591 None:intergenic 20.0%
!! CAAAATTAATTGTGAAGTTT+AGG - chr1_4:68955755-68955774 Msa0180950:five_prime_UTR 20.0%
! CTAAGGAATCATATATATGT+TGG + chr1_4:68954563-68954582 None:intergenic 25.0%
! TCAAAGACTTTCCTAATTAT+TGG + chr1_4:68954589-68954608 None:intergenic 25.0%
! TATATAGATCGTACAAATCA+TGG - chr1_4:68954799-68954818 Msa0180950:three_prime_UTR 25.0%
! ATAAGAAGAAAATGGTTCAT+AGG - chr1_4:68954845-68954864 Msa0180950:CDS 25.0%
! TAAGAAGAAAATGGTTCATA+GGG - chr1_4:68954846-68954865 Msa0180950:CDS 25.0%
! TTTATCTGATCAAATGAATG+AGG - chr1_4:68955049-68955068 Msa0180950:CDS 25.0%
! AAATCACTTGAAGAAGAATA+TGG - chr1_4:68955085-68955104 Msa0180950:CDS 25.0%
!!! ACTAAATGATGGATTCATAT+TGG + chr1_4:68955520-68955539 None:intergenic 25.0%
! TAATCTGAACAACTAAATGA+TGG + chr1_4:68955531-68955550 None:intergenic 25.0%
! TTATATTCTCTCTTTCTAAC+TGG - chr1_4:68955593-68955612 Msa0180950:CDS 25.0%
! ATGTAAATATTGAATCCATG+TGG - chr1_4:68955653-68955672 Msa0180950:CDS 25.0%
! TGTAAATATTGAATCCATGT+GGG - chr1_4:68955654-68955673 Msa0180950:CDS 25.0%
! CTAAGGAATCATATATATGT+TGG + chr1_4:68954563-68954582 None:intergenic 25.0%
! TCAAAGACTTTCCTAATTAT+TGG + chr1_4:68954589-68954608 None:intergenic 25.0%
! TATATAGATCGTACAAATCA+TGG - chr1_4:68954799-68954818 Msa0180950:three_prime_UTR 25.0%
! ATAAGAAGAAAATGGTTCAT+AGG - chr1_4:68954845-68954864 Msa0180950:CDS 25.0%
! TAAGAAGAAAATGGTTCATA+GGG - chr1_4:68954846-68954865 Msa0180950:CDS 25.0%
! TTTATCTGATCAAATGAATG+AGG - chr1_4:68955049-68955068 Msa0180950:CDS 25.0%
! AAATCACTTGAAGAAGAATA+TGG - chr1_4:68955085-68955104 Msa0180950:CDS 25.0%
!!! ACTAAATGATGGATTCATAT+TGG + chr1_4:68955520-68955539 None:intergenic 25.0%
! TAATCTGAACAACTAAATGA+TGG + chr1_4:68955531-68955550 None:intergenic 25.0%
! TTATATTCTCTCTTTCTAAC+TGG - chr1_4:68955593-68955612 Msa0180950:CDS 25.0%
! ATGTAAATATTGAATCCATG+TGG - chr1_4:68955653-68955672 Msa0180950:CDS 25.0%
! TGTAAATATTGAATCCATGT+GGG - chr1_4:68955654-68955673 Msa0180950:CDS 25.0%
AAGACTTTCCTAATTATTGG+TGG + chr1_4:68954586-68954605 None:intergenic 30.0%
TAAAGGAAACATGTTTGGTT+TGG + chr1_4:68954617-68954636 None:intergenic 30.0%
TGGTTGAGACTATTAGATAA+GGG + chr1_4:68954664-68954683 None:intergenic 30.0%
!! AAAATAATTTGTTTGGTGGC+CGG + chr1_4:68954780-68954799 None:intergenic 30.0%
TGGAAGAACTAAGTTACAAA+AGG - chr1_4:68955105-68955124 Msa0180950:CDS 30.0%
GGAAGAACTAAGTTACAAAA+GGG - chr1_4:68955106-68955125 Msa0180950:CDS 30.0%
!!! TGGCTTTTATTTTTAAGGCT+AGG + chr1_4:68955155-68955174 None:intergenic 30.0%
TAGTGAAACTACAAGAAACA+TGG + chr1_4:68955175-68955194 None:intergenic 30.0%
CACAAGAATAATAGAACTGT+GGG - chr1_4:68955197-68955216 Msa0180950:CDS 30.0%
TACTAGTACTTGCTTTATAG+AGG - chr1_4:68955232-68955251 Msa0180950:CDS 30.0%
TTCCAGAAATTTAGAAAGTG+GGG + chr1_4:68955264-68955283 None:intergenic 30.0%
GTTCCAGAAATTTAGAAAGT+GGG + chr1_4:68955265-68955284 None:intergenic 30.0%
TGTTCCAGAAATTTAGAAAG+TGG + chr1_4:68955266-68955285 None:intergenic 30.0%
! TGCTTTTCTATTCAAGTCAA+TGG - chr1_4:68955317-68955336 Msa0180950:intron 30.0%
AAGACTTTCCTAATTATTGG+TGG + chr1_4:68954586-68954605 None:intergenic 30.0%
TAAAGGAAACATGTTTGGTT+TGG + chr1_4:68954617-68954636 None:intergenic 30.0%
TGGTTGAGACTATTAGATAA+GGG + chr1_4:68954664-68954683 None:intergenic 30.0%
!! AAAATAATTTGTTTGGTGGC+CGG + chr1_4:68954780-68954799 None:intergenic 30.0%
TGGAAGAACTAAGTTACAAA+AGG - chr1_4:68955105-68955124 Msa0180950:CDS 30.0%
GGAAGAACTAAGTTACAAAA+GGG - chr1_4:68955106-68955125 Msa0180950:CDS 30.0%
!!! TGGCTTTTATTTTTAAGGCT+AGG + chr1_4:68955155-68955174 None:intergenic 30.0%
TAGTGAAACTACAAGAAACA+TGG + chr1_4:68955175-68955194 None:intergenic 30.0%
CACAAGAATAATAGAACTGT+GGG - chr1_4:68955197-68955216 Msa0180950:CDS 30.0%
TACTAGTACTTGCTTTATAG+AGG - chr1_4:68955232-68955251 Msa0180950:CDS 30.0%
TTCCAGAAATTTAGAAAGTG+GGG + chr1_4:68955264-68955283 None:intergenic 30.0%
GTTCCAGAAATTTAGAAAGT+GGG + chr1_4:68955265-68955284 None:intergenic 30.0%
TGTTCCAGAAATTTAGAAAG+TGG + chr1_4:68955266-68955285 None:intergenic 30.0%
! TGCTTTTCTATTCAAGTCAA+TGG - chr1_4:68955317-68955336 Msa0180950:intron 30.0%
TTCCTTAGCCACCAATAATT+AGG - chr1_4:68954575-68954594 Msa0180950:three_prime_UTR 35.0%
! TTCCTAATTATTGGTGGCTA+AGG + chr1_4:68954580-68954599 None:intergenic 35.0%
CGTTGTAAAGGAAACATGTT+TGG + chr1_4:68954622-68954641 None:intergenic 35.0%
AGTTCATTGCATCGTTGTAA+AGG + chr1_4:68954634-68954653 None:intergenic 35.0%
TTTACAACGATGCAATGAAC+TGG - chr1_4:68954633-68954652 Msa0180950:three_prime_UTR 35.0%
GTGGTTGAGACTATTAGATA+AGG + chr1_4:68954665-68954684 None:intergenic 35.0%
ATAGGGAAATTGAGAAGCAA+AGG - chr1_4:68954863-68954882 Msa0180950:CDS 35.0%
CCACAGTTCTATTATTCTTG+TGG + chr1_4:68955199-68955218 None:intergenic 35.0%
CCACAAGAATAATAGAACTG+TGG - chr1_4:68955196-68955215 Msa0180950:CDS 35.0%
AGTACTTGCTTTATAGAGGA+AGG - chr1_4:68955236-68955255 Msa0180950:CDS 35.0%
CTGGAACAACTGATGAAATA+TGG - chr1_4:68955278-68955297 Msa0180950:CDS 35.0%
! TATTGAGCTTGATGATTCAC+AGG - chr1_4:68955446-68955465 Msa0180950:CDS 35.0%
TAGATCTAACTGAGCTAAGA+AGG - chr1_4:68955487-68955506 Msa0180950:CDS 35.0%
TTCCTTAGCCACCAATAATT+AGG - chr1_4:68954575-68954594 Msa0180950:three_prime_UTR 35.0%
! TTCCTAATTATTGGTGGCTA+AGG + chr1_4:68954580-68954599 None:intergenic 35.0%
CGTTGTAAAGGAAACATGTT+TGG + chr1_4:68954622-68954641 None:intergenic 35.0%
AGTTCATTGCATCGTTGTAA+AGG + chr1_4:68954634-68954653 None:intergenic 35.0%
TTTACAACGATGCAATGAAC+TGG - chr1_4:68954633-68954652 Msa0180950:three_prime_UTR 35.0%
GTGGTTGAGACTATTAGATA+AGG + chr1_4:68954665-68954684 None:intergenic 35.0%
ATAGGGAAATTGAGAAGCAA+AGG - chr1_4:68954863-68954882 Msa0180950:CDS 35.0%
CCACAGTTCTATTATTCTTG+TGG + chr1_4:68955199-68955218 None:intergenic 35.0%
CCACAAGAATAATAGAACTG+TGG - chr1_4:68955196-68955215 Msa0180950:CDS 35.0%
AGTACTTGCTTTATAGAGGA+AGG - chr1_4:68955236-68955255 Msa0180950:CDS 35.0%
CTGGAACAACTGATGAAATA+TGG - chr1_4:68955278-68955297 Msa0180950:CDS 35.0%
! TATTGAGCTTGATGATTCAC+AGG - chr1_4:68955446-68955465 Msa0180950:CDS 35.0%
TAGATCTAACTGAGCTAAGA+AGG - chr1_4:68955487-68955506 Msa0180950:CDS 35.0%
! AGATTTTGTGATGTTGTGGG+TGG + chr1_4:68954687-68954706 None:intergenic 40.0%
! CCGAGATTTTGTGATGTTGT+GGG + chr1_4:68954690-68954709 None:intergenic 40.0%
CCCACAACATCACAAAATCT+CGG - chr1_4:68954687-68954706 Msa0180950:three_prime_UTR 40.0%
! TCCGAGATTTTGTGATGTTG+TGG + chr1_4:68954691-68954710 None:intergenic 40.0%
GAAGCAAAGGAGACAAGAAA+TGG - chr1_4:68954876-68954895 Msa0180950:CDS 40.0%
TCACCTTGATGAAGTGAAGA+GGG + chr1_4:68954933-68954952 None:intergenic 40.0%
TTCACCTTGATGAAGTGAAG+AGG + chr1_4:68954934-68954953 None:intergenic 40.0%
TGCCCCACTTTCTAAATTTC+TGG - chr1_4:68955259-68955278 Msa0180950:CDS 40.0%
CTCAGTTAGATCTACACTGT+CGG + chr1_4:68955481-68955500 None:intergenic 40.0%
! TTTGGTTTCTGCTTCTGTCT+TGG - chr1_4:68955682-68955701 Msa0180950:CDS 40.0%
TTGGTTTCTGCTTCTGTCTT+GGG - chr1_4:68955683-68955702 Msa0180950:CDS 40.0%
! AGATTTTGTGATGTTGTGGG+TGG + chr1_4:68954687-68954706 None:intergenic 40.0%
! CCGAGATTTTGTGATGTTGT+GGG + chr1_4:68954690-68954709 None:intergenic 40.0%
CCCACAACATCACAAAATCT+CGG - chr1_4:68954687-68954706 Msa0180950:three_prime_UTR 40.0%
! TCCGAGATTTTGTGATGTTG+TGG + chr1_4:68954691-68954710 None:intergenic 40.0%
GAAGCAAAGGAGACAAGAAA+TGG - chr1_4:68954876-68954895 Msa0180950:CDS 40.0%
TCACCTTGATGAAGTGAAGA+GGG + chr1_4:68954933-68954952 None:intergenic 40.0%
TTCACCTTGATGAAGTGAAG+AGG + chr1_4:68954934-68954953 None:intergenic 40.0%
TGCCCCACTTTCTAAATTTC+TGG - chr1_4:68955259-68955278 Msa0180950:CDS 40.0%
CTCAGTTAGATCTACACTGT+CGG + chr1_4:68955481-68955500 None:intergenic 40.0%
! TTTGGTTTCTGCTTCTGTCT+TGG - chr1_4:68955682-68955701 Msa0180950:CDS 40.0%
TTGGTTTCTGCTTCTGTCTT+GGG - chr1_4:68955683-68955702 Msa0180950:CDS 40.0%
! TTTTGTGATGTTGTGGGTGG+TGG + chr1_4:68954684-68954703 None:intergenic 45.0%
AAGAGGGAGAAGGGATCTAA+GGG + chr1_4:68954917-68954936 None:intergenic 45.0%
GATGAAGTGAAGAGGGAGAA+GGG + chr1_4:68954926-68954945 None:intergenic 45.0%
TGATGAAGTGAAGAGGGAGA+AGG + chr1_4:68954927-68954946 None:intergenic 45.0%
TCTCCCTCTTCACTTCATCA+AGG - chr1_4:68954927-68954946 Msa0180950:intron 45.0%
ACTACATAGTGTGCAGTGTG+AGG - chr1_4:68955346-68955365 Msa0180950:intron 45.0%
! TTTTGTGATGTTGTGGGTGG+TGG + chr1_4:68954684-68954703 None:intergenic 45.0%
AAGAGGGAGAAGGGATCTAA+GGG + chr1_4:68954917-68954936 None:intergenic 45.0%
GATGAAGTGAAGAGGGAGAA+GGG + chr1_4:68954926-68954945 None:intergenic 45.0%
TGATGAAGTGAAGAGGGAGA+AGG + chr1_4:68954927-68954946 None:intergenic 45.0%
TCTCCCTCTTCACTTCATCA+AGG - chr1_4:68954927-68954946 Msa0180950:intron 45.0%
ACTACATAGTGTGCAGTGTG+AGG - chr1_4:68955346-68955365 Msa0180950:intron 45.0%
GAAGAGGGAGAAGGGATCTA+AGG + chr1_4:68954918-68954937 None:intergenic 50.0%
GAATCCATGTGGGCTCTCTT+TGG - chr1_4:68955664-68955683 Msa0180950:CDS 50.0%
GAAACCAAAGAGAGCCCACA+TGG + chr1_4:68955671-68955690 None:intergenic 50.0%
GAAGAGGGAGAAGGGATCTA+AGG + chr1_4:68954918-68954937 None:intergenic 50.0%
GAATCCATGTGGGCTCTCTT+TGG - chr1_4:68955664-68955683 Msa0180950:CDS 50.0%
GAAACCAAAGAGAGCCCACA+TGG + chr1_4:68955671-68955690 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 68954552 68955821 68954552 ID=Msa0180950;Name=Msa0180950
chr1_4 mRNA 68954552 68955821 68954552 ID=Msa0180950-mRNA-1;Parent=Msa0180950;Name=Msa0180950-mRNA-1;_AED=0.02;_eAED=0.02;_QI=73|1|1|1|1|1|3|270|245
chr1_4 exon 68954552 68954905 68954552 ID=Msa0180950-mRNA-1:exon:22900;Parent=Msa0180950-mRNA-1
chr1_4 exon 68955006 68955335 68955006 ID=Msa0180950-mRNA-1:exon:22899;Parent=Msa0180950-mRNA-1
chr1_4 exon 68955425 68955821 68955425 ID=Msa0180950-mRNA-1:exon:22898;Parent=Msa0180950-mRNA-1
chr1_4 five_prime_UTR 68955749 68955821 68955749 ID=Msa0180950-mRNA-1:five_prime_utr;Parent=Msa0180950-mRNA-1
chr1_4 CDS 68955425 68955748 68955425 ID=Msa0180950-mRNA-1:cds;Parent=Msa0180950-mRNA-1
chr1_4 CDS 68955006 68955335 68955006 ID=Msa0180950-mRNA-1:cds;Parent=Msa0180950-mRNA-1
chr1_4 CDS 68954822 68954905 68954822 ID=Msa0180950-mRNA-1:cds;Parent=Msa0180950-mRNA-1
chr1_4 three_prime_UTR 68954552 68954821 68954552 ID=Msa0180950-mRNA-1:three_prime_utr;Parent=Msa0180950-mRNA-1
Gene Sequence

>Msa0180950

ATGTTTCCTTTACAACGATGCAATGAACTGGCGAAACCCTTATCTAATAGTCTCAACCACCACCCACAACATCACAAAATCTCGGAAGATCTGATTCTTGATGATTGTGATTCTCTTGTAATTGATTTTTCTCTTAAAAAAATGGATACTAACCGGCCACCAAACAAATTATTTTATATAGATCGTACAAATCATGGTAGTAATCGTAATTCTATTGAAGATAAGAAGAAAATGGTTCATAGGGAAATTGAGAAGCAAAGGAGACAAGAAATGGCTACACTTCATGCTTCCCTTAGATCCCTTCTCCCTCTTCACTTCATCAAGGGAAAACGTTCTTTATCTGATCAAATGAATGAGGCAGTGAATTACATAAATCACTTGAAGAAGAATATGGAAGAACTAAGTTACAAAAGGGATGAACTGAAAAAACTCTCTAATCCTAGCCTTAAAAATAAAAGCCATGTTTCTTGTAGTTTCACTATCCACAAGAATAATAGAACTGTGGGAATTGAAATCAGTACTAGTACTTGCTTTATAGAGGAAGGTGCCCCACTTTCTAAATTTCTGGAACAACTGATGAAATATGGACTTGATGTTGTTAGCTGCTTTTCTATTCAAGTCAATGGAAGATTACTACATAGTGTGCAGTGTGAGGTTATCGATTCCGACAGTGTAGATCTAACTGAGCTAAGAAGGAATTTTTCCAATATGAATCCATCATTTAGTTGTTCAGATTAA
Protein sequence

>Msa0180950

MFPLQRCNELAKPLSNSLNHHPQHHKISEDLILDDCDSLVIDFSLKKMDTNRPPNKLFYIDRTNHGSNRNSIEDKKKMVHREIEKQRRQEMATLHASLRSLLPLHFIKGKRSLSDQMNEAVNYINHLKKNMEELSYKRDELKKLSNPSLKNKSHVSCSFTIHKNNRTVGIEISTSTCFIEEGAPLSKFLEQLMKYGLDVVSCFSIQVNGRLLHSVQCEVIDSDSVDLTELRRNFSNMNPSFSCSD*