AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0040150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0040150 MtrunA17_Chr1g0204791 91.057 123 9 2 1 122 1 122 8.53e-77 223
Msa0040150 MtrunA17_Chr5g0432501 37.069 116 66 2 14 122 21 136 2.59e-21 82.8
Msa0040150 MtrunA17_Chr8g0342581 35.200 125 58 4 20 122 6 129 8.07e-15 65.9
Msa0040150 MtrunA17_Chr4g0019101 42.105 76 39 2 47 122 92 162 1.22e-13 63.5
Msa0040150 MtrunA17_Chr4g0019111 42.188 64 35 1 59 122 16 77 7.78e-11 54.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0040150 AT3G22820.1 46.729 107 54 1 16 122 4 107 1.15e-27 98.2
Msa0040150 AT4G14723.1 49.524 105 40 3 19 122 17 109 6.24e-27 96.7
Msa0040150 AT2G30370.2 44.828 116 51 4 16 122 45 156 7.87e-23 87.4
Msa0040150 AT2G30370.3 44.828 116 51 4 16 122 55 166 1.02e-22 87.4
Msa0040150 AT2G30370.1 51.190 84 33 2 47 122 147 230 1.06e-21 86.3

Find 75 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCTCTCCTTCCCTCTTT+AGG 0.211079 1_1:+70183492 Msa0040150:five_prime_UTR
TTGTAATTATATGAATTATT+TGG 0.238057 1_1:+70184677 Msa0040150:three_prime_UTR
CACTTGCAACGCCATGCTTC+TGG 0.251321 1_1:-70184141 None:intergenic
TGCTCTGAGGTTTGAATTGT+TGG 0.274165 1_1:+70184382 Msa0040150:three_prime_UTR
TTTGGGACTTGGGTTGGGTT+TGG 0.275308 1_1:+70184491 Msa0040150:three_prime_UTR
CATTGGGAATGTAAGATGTT+TGG 0.278233 1_1:+70184473 Msa0040150:three_prime_UTR
CCAATGAAGAAGCCTTAAAA+AGG 0.292236 1_1:-70184456 None:intergenic
AAGCAATGGTGGTGGTGTTT+AGG 0.302399 1_1:-70183761 None:intergenic
ATTGGGAATGTAAGATGTTT+GGG 0.318124 1_1:+70184474 Msa0040150:three_prime_UTR
AACACCACCACCATTGCTTC+TGG 0.327365 1_1:+70183765 Msa0040150:CDS
AGCAATGGTGGTGGTGTTTA+GGG 0.333049 1_1:-70183760 None:intergenic
ATGATTAAACCGGGGTGAAC+CGG 0.350078 1_1:-70184102 None:intergenic
AGATGTTTGGGACTTGGGTT+GGG 0.351631 1_1:+70184486 Msa0040150:three_prime_UTR
GCGGAGCAGAAGAAGGTTGC+TGG 0.381489 1_1:+70184009 Msa0040150:CDS
CAGAAGAAGGTTGCTGGGCC+AGG 0.385682 1_1:+70184015 Msa0040150:CDS
AAACGTTGCCAGAACCGAAC+AGG 0.401440 1_1:+70184266 Msa0040150:three_prime_UTR
ATGTAAGATGTTTGGGACTT+GGG 0.402279 1_1:+70184481 Msa0040150:three_prime_UTR
CTCAAGACGTATGATTAAAC+CGG 0.405928 1_1:-70184112 None:intergenic
GAGAAGAAATGAACCTAAAG+AGG 0.416992 1_1:-70183505 None:intergenic
GGGTGAACCGGAACGTGAAC+CGG 0.417630 1_1:-70184090 None:intergenic
AAGATGTTTGGGACTTGGGT+TGG 0.421077 1_1:+70184485 Msa0040150:three_prime_UTR
AATGTAAGATGTTTGGGACT+TGG 0.424168 1_1:+70184480 Msa0040150:three_prime_UTR
GAGGTTTGAATTGTTGGACT+TGG 0.433808 1_1:+70184388 Msa0040150:three_prime_UTR
TGAGAGTGAGAAAGAGAGAA+TGG 0.434630 1_1:-70183648 None:intergenic
ATGTCAACCGGTTCACGTTC+CGG 0.448654 1_1:+70184083 Msa0040150:CDS
AACAGTGAAGCATCAAGAAA+TGG 0.452869 1_1:+70183945 Msa0040150:CDS
GTTTGAATTGTTGGACTTGG+TGG 0.458192 1_1:+70184391 Msa0040150:three_prime_UTR
AAGCATCAAGAAATGGAAAA+CGG 0.474883 1_1:+70183952 Msa0040150:CDS
CTCTTCTTCCACGGTGATGT+CGG 0.479412 1_1:-70183979 None:intergenic
AATGACAAAATACTATGTTA+AGG 0.481772 1_1:+70184640 Msa0040150:three_prime_UTR
AGCATCAAGAAATGGAAAAC+GGG 0.486636 1_1:+70183953 Msa0040150:CDS
CGTGTAGATCCAAGTGTGAT+TGG 0.513041 1_1:+70184052 Msa0040150:CDS
GAAGCATGGCGTTGCAAGTG+TGG 0.519320 1_1:+70184144 Msa0040150:CDS
AGAGGTGGCGGAGCAGAAGA+AGG 0.519433 1_1:+70184002 Msa0040150:CDS
TTGGTGTAACCCATGTCAAC+CGG 0.519681 1_1:+70184071 Msa0040150:CDS
GGAACGTGAACCGGTTGACA+TGG 0.526123 1_1:-70184081 None:intergenic
AGTGAGAAAGAGAGAATGGA+AGG 0.528050 1_1:-70183644 None:intergenic
TACTTATCAATTCATCATCA+AGG 0.529658 1_1:-70184183 None:intergenic
GCCACCTCTCTCTTCTTCCA+CGG 0.532769 1_1:-70183988 None:intergenic
CGGAGCAGAAGAAGGTTGCT+GGG 0.544379 1_1:+70184010 Msa0040150:CDS
GTATTATTAAATGTTACTGC+AGG 0.545034 1_1:-70183615 None:intergenic
GTGGAAGAAGAGAGAGGTGG+CGG 0.545993 1_1:+70183990 Msa0040150:CDS
TTGTGGTGCGAGAAGAGTTA+TGG 0.548135 1_1:-70183831 None:intergenic
AGAGTACAGAGAAGATGAAG+AGG 0.551498 1_1:-70183679 None:intergenic
AACTTGAACTTACTAGGTTG+TGG 0.559169 1_1:-70183848 None:intergenic
AGAAGAAGGTTGCTGGGCCA+GGG 0.561033 1_1:+70184016 Msa0040150:CDS
AAGAGAGAATGGAAGGTGTG+TGG 0.576934 1_1:-70183637 None:intergenic
GGTTCTCTGTTGTGTAGAGA+GGG 0.587339 1_1:-70184703 None:intergenic
TGGTTCTCTGTTGTGTAGAG+AGG 0.588574 1_1:-70184704 None:intergenic
GAAATGAACCTAAAGAGGGA+AGG 0.600783 1_1:-70183500 None:intergenic
TCAAGACGTATGATTAAACC+GGG 0.608793 1_1:-70184111 None:intergenic
TGGGTTACACCAATCACACT+TGG 0.609884 1_1:-70184061 None:intergenic
AAGCATGGCGTTGCAAGTGT+GGG 0.610762 1_1:+70184145 Msa0040150:CDS
AGAAGAAATGAACCTAAAGA+GGG 0.612120 1_1:-70183504 None:intergenic
CACGACGGAGGTGTTGACCC+TGG 0.613267 1_1:-70184033 None:intergenic
GAACGTGAACCGGTTGACAT+GGG 0.614728 1_1:-70184080 None:intergenic
GTACGCACATATTGATGAGG+TGG 0.617360 1_1:-70183730 None:intergenic
ATAGAAGAAGAATAACAAGA+TGG 0.617872 1_1:-70184724 None:intergenic
TTGAGTATTATCCAGAAGCA+TGG 0.618402 1_1:+70184130 Msa0040150:CDS
TACTACTATGTACAATAGAG+AGG 0.621909 1_1:-70184543 None:intergenic
AACGTTGCCAGAACCGAACA+GGG 0.633112 1_1:+70184267 Msa0040150:three_prime_UTR
TGGGTAATAACCAGAAGCAA+TGG 0.633930 1_1:-70183775 None:intergenic
GTTCACGTTCCGGTTCACCC+CGG 0.635691 1_1:+70184093 Msa0040150:CDS
ATAACCAGAAGCAATGGTGG+TGG 0.639918 1_1:-70183769 None:intergenic
GTAATAACCAGAAGCAATGG+TGG 0.650622 1_1:-70183772 None:intergenic
AATGGTGGTGGTGTTTAGGG+TGG 0.653961 1_1:-70183757 None:intergenic
ACTACTATGTACAATAGAGA+GGG 0.659336 1_1:-70184542 None:intergenic
GCAGTACGCACATATTGATG+AGG 0.669919 1_1:-70183733 None:intergenic
GTATTAATGATGTTGCTCTG+AGG 0.673923 1_1:+70184369 Msa0040150:three_prime_UTR
ACCGTGGAAGAAGAGAGAGG+TGG 0.701228 1_1:+70183987 Msa0040150:CDS
ATCACCGTGGAAGAAGAGAG+AGG 0.712922 1_1:+70183984 Msa0040150:CDS
CACTTGGATCTACACGACGG+AGG 0.750576 1_1:-70184045 None:intergenic
TCACACTTGGATCTACACGA+CGG 0.754283 1_1:-70184048 None:intergenic
ACGGGTCTCCGACATCACCG+TGG 0.754489 1_1:+70183971 Msa0040150:CDS
CAAGACGTATGATTAAACCG+GGG 0.816477 1_1:-70184110 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTACAATAAAAACATTAAA+AGG - chr1_1:70184618-70184637 None:intergenic 10.0%
!! TTGTAATTATATGAATTATT+TGG + chr1_1:70184677-70184696 Msa0040150:three_prime_UTR 10.0%
!! TTTACAATAAAAACATTAAA+AGG - chr1_1:70184618-70184637 None:intergenic 10.0%
!! TTGTAATTATATGAATTATT+TGG + chr1_1:70184677-70184696 Msa0040150:three_prime_UTR 10.0%
!!! TGAAAAAAGTATGAAGAAAA+TGG - chr1_1:70183889-70183908 None:intergenic 20.0%
!!! TGAGTACTAATATTGATTTT+TGG + chr1_1:70184428-70184447 Msa0040150:three_prime_UTR 20.0%
!!! TTTTTGGTTTAACCTTTTTA+AGG + chr1_1:70184444-70184463 Msa0040150:three_prime_UTR 20.0%
!!! TCTATTTCCTTTTTTGTTTT+AGG + chr1_1:70184573-70184592 Msa0040150:three_prime_UTR 20.0%
!! ACATAAACCTAAAACAAAAA+AGG - chr1_1:70184583-70184602 None:intergenic 20.0%
!! ACAATAAAAACATTAAAAGG+AGG - chr1_1:70184615-70184634 None:intergenic 20.0%
!! AATGACAAAATACTATGTTA+AGG + chr1_1:70184640-70184659 Msa0040150:three_prime_UTR 20.0%
!!! TGAAAAAAGTATGAAGAAAA+TGG - chr1_1:70183889-70183908 None:intergenic 20.0%
!!! TGAGTACTAATATTGATTTT+TGG + chr1_1:70184428-70184447 Msa0040150:three_prime_UTR 20.0%
!!! TTTTTGGTTTAACCTTTTTA+AGG + chr1_1:70184444-70184463 Msa0040150:three_prime_UTR 20.0%
!!! TCTATTTCCTTTTTTGTTTT+AGG + chr1_1:70184573-70184592 Msa0040150:three_prime_UTR 20.0%
!! ACATAAACCTAAAACAAAAA+AGG - chr1_1:70184583-70184602 None:intergenic 20.0%
!! ACAATAAAAACATTAAAAGG+AGG - chr1_1:70184615-70184634 None:intergenic 20.0%
!! AATGACAAAATACTATGTTA+AGG + chr1_1:70184640-70184659 Msa0040150:three_prime_UTR 20.0%
! GTATTATTAAATGTTACTGC+AGG - chr1_1:70183618-70183637 None:intergenic 25.0%
! AGAAAACAAGAGAAAAATGA+AGG - chr1_1:70183801-70183820 None:intergenic 25.0%
! TACTTATCAATTCATCATCA+AGG - chr1_1:70184186-70184205 None:intergenic 25.0%
!!! GTGTTTTTTTCTTTTGTACT+AGG - chr1_1:70184246-70184265 None:intergenic 25.0%
! AAAAGGGGAAAAATTTAAGA+TGG - chr1_1:70184310-70184329 None:intergenic 25.0%
!! ATCTTAAATTTTTCCCCTTT+TGG + chr1_1:70184309-70184328 Msa0040150:three_prime_UTR 25.0%
!! TCTTAAATTTTTCCCCTTTT+GGG + chr1_1:70184310-70184329 Msa0040150:three_prime_UTR 25.0%
! TAATACAAAAAAGCCCAAAA+GGG - chr1_1:70184326-70184345 None:intergenic 25.0%
! ATAATACAAAAAAGCCCAAA+AGG - chr1_1:70184327-70184346 None:intergenic 25.0%
! GTATTATTAAATGTTACTGC+AGG - chr1_1:70183618-70183637 None:intergenic 25.0%
! AGAAAACAAGAGAAAAATGA+AGG - chr1_1:70183801-70183820 None:intergenic 25.0%
! TACTTATCAATTCATCATCA+AGG - chr1_1:70184186-70184205 None:intergenic 25.0%
!!! GTGTTTTTTTCTTTTGTACT+AGG - chr1_1:70184246-70184265 None:intergenic 25.0%
! AAAAGGGGAAAAATTTAAGA+TGG - chr1_1:70184310-70184329 None:intergenic 25.0%
!! ATCTTAAATTTTTCCCCTTT+TGG + chr1_1:70184309-70184328 Msa0040150:three_prime_UTR 25.0%
!! TCTTAAATTTTTCCCCTTTT+GGG + chr1_1:70184310-70184329 Msa0040150:three_prime_UTR 25.0%
! TAATACAAAAAAGCCCAAAA+GGG - chr1_1:70184326-70184345 None:intergenic 25.0%
! ATAATACAAAAAAGCCCAAA+AGG - chr1_1:70184327-70184346 None:intergenic 25.0%
AGAAGAAATGAACCTAAAGA+GGG - chr1_1:70183507-70183526 None:intergenic 30.0%
!! TCATTTCTTCTCTTTTGACT+TGG + chr1_1:70183516-70183535 Msa0040150:five_prime_UTR 30.0%
AACAAGAGAAAAATGAAGGT+GGG - chr1_1:70183797-70183816 None:intergenic 30.0%
AAACAAGAGAAAAATGAAGG+TGG - chr1_1:70183798-70183817 None:intergenic 30.0%
GAAATGAACTTGAACTTACT+AGG - chr1_1:70183857-70183876 None:intergenic 30.0%
! CAACAACTCTTTTCATTTTC+AGG + chr1_1:70183913-70183932 Msa0040150:intron 30.0%
!!! TTTTCATTTTCAGGTAGTAG+TGG + chr1_1:70183922-70183941 Msa0040150:intron 30.0%
AAGCATCAAGAAATGGAAAA+CGG + chr1_1:70183952-70183971 Msa0040150:CDS 30.0%
AATACAAAAAAGCCCAAAAG+GGG - chr1_1:70184325-70184344 None:intergenic 30.0%
!!! CTTTTTAAGGCTTCTTCATT+GGG + chr1_1:70184457-70184476 Msa0040150:three_prime_UTR 30.0%
ATTGGGAATGTAAGATGTTT+GGG + chr1_1:70184474-70184493 Msa0040150:three_prime_UTR 30.0%
ACTACTATGTACAATAGAGA+GGG - chr1_1:70184545-70184564 None:intergenic 30.0%
TACTACTATGTACAATAGAG+AGG - chr1_1:70184546-70184565 None:intergenic 30.0%
! ATATCTTGTTTGAGTATCTG+AGG - chr1_1:70183470-70183489 None:intergenic 30.0%
AGAAGAAATGAACCTAAAGA+GGG - chr1_1:70183507-70183526 None:intergenic 30.0%
!! TCATTTCTTCTCTTTTGACT+TGG + chr1_1:70183516-70183535 Msa0040150:five_prime_UTR 30.0%
AACAAGAGAAAAATGAAGGT+GGG - chr1_1:70183797-70183816 None:intergenic 30.0%
AAACAAGAGAAAAATGAAGG+TGG - chr1_1:70183798-70183817 None:intergenic 30.0%
GAAATGAACTTGAACTTACT+AGG - chr1_1:70183857-70183876 None:intergenic 30.0%
! CAACAACTCTTTTCATTTTC+AGG + chr1_1:70183913-70183932 Msa0040150:intron 30.0%
!!! TTTTCATTTTCAGGTAGTAG+TGG + chr1_1:70183922-70183941 Msa0040150:intron 30.0%
AAGCATCAAGAAATGGAAAA+CGG + chr1_1:70183952-70183971 Msa0040150:CDS 30.0%
AATACAAAAAAGCCCAAAAG+GGG - chr1_1:70184325-70184344 None:intergenic 30.0%
!!! CTTTTTAAGGCTTCTTCATT+GGG + chr1_1:70184457-70184476 Msa0040150:three_prime_UTR 30.0%
ATTGGGAATGTAAGATGTTT+GGG + chr1_1:70184474-70184493 Msa0040150:three_prime_UTR 30.0%
ACTACTATGTACAATAGAGA+GGG - chr1_1:70184545-70184564 None:intergenic 30.0%
TACTACTATGTACAATAGAG+AGG - chr1_1:70184546-70184565 None:intergenic 30.0%
GAGAAGAAATGAACCTAAAG+AGG - chr1_1:70183508-70183527 None:intergenic 35.0%
AACTTGAACTTACTAGGTTG+TGG - chr1_1:70183851-70183870 None:intergenic 35.0%
! AACAGTGAAGCATCAAGAAA+TGG + chr1_1:70183945-70183964 Msa0040150:CDS 35.0%
AGCATCAAGAAATGGAAAAC+GGG + chr1_1:70183953-70183972 Msa0040150:CDS 35.0%
TCAAGACGTATGATTAAACC+GGG - chr1_1:70184114-70184133 None:intergenic 35.0%
CTCAAGACGTATGATTAAAC+CGG - chr1_1:70184115-70184134 None:intergenic 35.0%
TTGAGTATTATCCAGAAGCA+TGG + chr1_1:70184130-70184149 Msa0040150:CDS 35.0%
GTATTAATGATGTTGCTCTG+AGG + chr1_1:70184369-70184388 Msa0040150:three_prime_UTR 35.0%
CCAATGAAGAAGCCTTAAAA+AGG - chr1_1:70184459-70184478 None:intergenic 35.0%
!!! CCTTTTTAAGGCTTCTTCAT+TGG + chr1_1:70184456-70184475 Msa0040150:three_prime_UTR 35.0%
CATTGGGAATGTAAGATGTT+TGG + chr1_1:70184473-70184492 Msa0040150:three_prime_UTR 35.0%
! AATGTAAGATGTTTGGGACT+TGG + chr1_1:70184480-70184499 Msa0040150:three_prime_UTR 35.0%
! ATGTAAGATGTTTGGGACTT+GGG + chr1_1:70184481-70184500 Msa0040150:three_prime_UTR 35.0%
GAGAAGAAATGAACCTAAAG+AGG - chr1_1:70183508-70183527 None:intergenic 35.0%
AACTTGAACTTACTAGGTTG+TGG - chr1_1:70183851-70183870 None:intergenic 35.0%
! AACAGTGAAGCATCAAGAAA+TGG + chr1_1:70183945-70183964 Msa0040150:CDS 35.0%
AGCATCAAGAAATGGAAAAC+GGG + chr1_1:70183953-70183972 Msa0040150:CDS 35.0%
TCAAGACGTATGATTAAACC+GGG - chr1_1:70184114-70184133 None:intergenic 35.0%
CTCAAGACGTATGATTAAAC+CGG - chr1_1:70184115-70184134 None:intergenic 35.0%
TTGAGTATTATCCAGAAGCA+TGG + chr1_1:70184130-70184149 Msa0040150:CDS 35.0%
GTATTAATGATGTTGCTCTG+AGG + chr1_1:70184369-70184388 Msa0040150:three_prime_UTR 35.0%
CCAATGAAGAAGCCTTAAAA+AGG - chr1_1:70184459-70184478 None:intergenic 35.0%
!!! CCTTTTTAAGGCTTCTTCAT+TGG + chr1_1:70184456-70184475 Msa0040150:three_prime_UTR 35.0%
CATTGGGAATGTAAGATGTT+TGG + chr1_1:70184473-70184492 Msa0040150:three_prime_UTR 35.0%
! AATGTAAGATGTTTGGGACT+TGG + chr1_1:70184480-70184499 Msa0040150:three_prime_UTR 35.0%
! ATGTAAGATGTTTGGGACTT+GGG + chr1_1:70184481-70184500 Msa0040150:three_prime_UTR 35.0%
GAAATGAACCTAAAGAGGGA+AGG - chr1_1:70183503-70183522 None:intergenic 40.0%
! CTCACATTTTCACAACACAC+AGG + chr1_1:70183560-70183579 Msa0040150:five_prime_UTR 40.0%
AGTGAGAAAGAGAGAATGGA+AGG - chr1_1:70183647-70183666 None:intergenic 40.0%
TGAGAGTGAGAAAGAGAGAA+TGG - chr1_1:70183651-70183670 None:intergenic 40.0%
AGAGTACAGAGAAGATGAAG+AGG - chr1_1:70183682-70183701 None:intergenic 40.0%
GTAATAACCAGAAGCAATGG+TGG - chr1_1:70183775-70183794 None:intergenic 40.0%
TGGGTAATAACCAGAAGCAA+TGG - chr1_1:70183778-70183797 None:intergenic 40.0%
CAAGACGTATGATTAAACCG+GGG - chr1_1:70184113-70184132 None:intergenic 40.0%
! TCTGCAACATTTTCCCTGTT+CGG - chr1_1:70184283-70184302 None:intergenic 40.0%
!! TGCTCTGAGGTTTGAATTGT+TGG + chr1_1:70184382-70184401 Msa0040150:three_prime_UTR 40.0%
GAGGTTTGAATTGTTGGACT+TGG + chr1_1:70184388-70184407 Msa0040150:three_prime_UTR 40.0%
GTTTGAATTGTTGGACTTGG+TGG + chr1_1:70184391-70184410 Msa0040150:three_prime_UTR 40.0%
GAAATGAACCTAAAGAGGGA+AGG - chr1_1:70183503-70183522 None:intergenic 40.0%
! CTCACATTTTCACAACACAC+AGG + chr1_1:70183560-70183579 Msa0040150:five_prime_UTR 40.0%
AGTGAGAAAGAGAGAATGGA+AGG - chr1_1:70183647-70183666 None:intergenic 40.0%
TGAGAGTGAGAAAGAGAGAA+TGG - chr1_1:70183651-70183670 None:intergenic 40.0%
AGAGTACAGAGAAGATGAAG+AGG - chr1_1:70183682-70183701 None:intergenic 40.0%
GTAATAACCAGAAGCAATGG+TGG - chr1_1:70183775-70183794 None:intergenic 40.0%
TGGGTAATAACCAGAAGCAA+TGG - chr1_1:70183778-70183797 None:intergenic 40.0%
CAAGACGTATGATTAAACCG+GGG - chr1_1:70184113-70184132 None:intergenic 40.0%
! TCTGCAACATTTTCCCTGTT+CGG - chr1_1:70184283-70184302 None:intergenic 40.0%
!! TGCTCTGAGGTTTGAATTGT+TGG + chr1_1:70184382-70184401 Msa0040150:three_prime_UTR 40.0%
GAGGTTTGAATTGTTGGACT+TGG + chr1_1:70184388-70184407 Msa0040150:three_prime_UTR 40.0%
GTTTGAATTGTTGGACTTGG+TGG + chr1_1:70184391-70184410 Msa0040150:three_prime_UTR 40.0%
! AAGAGAGAATGGAAGGTGTG+TGG - chr1_1:70183640-70183659 None:intergenic 45.0%
GTACGCACATATTGATGAGG+TGG - chr1_1:70183733-70183752 None:intergenic 45.0%
GCAGTACGCACATATTGATG+AGG - chr1_1:70183736-70183755 None:intergenic 45.0%
!! AGCAATGGTGGTGGTGTTTA+GGG - chr1_1:70183763-70183782 None:intergenic 45.0%
!! AAGCAATGGTGGTGGTGTTT+AGG - chr1_1:70183764-70183783 None:intergenic 45.0%
ATAACCAGAAGCAATGGTGG+TGG - chr1_1:70183772-70183791 None:intergenic 45.0%
TTGTGGTGCGAGAAGAGTTA+TGG - chr1_1:70183834-70183853 None:intergenic 45.0%
TCACACTTGGATCTACACGA+CGG - chr1_1:70184051-70184070 None:intergenic 45.0%
CGTGTAGATCCAAGTGTGAT+TGG + chr1_1:70184052-70184071 Msa0040150:CDS 45.0%
TGGGTTACACCAATCACACT+TGG - chr1_1:70184064-70184083 None:intergenic 45.0%
TTGGTGTAACCCATGTCAAC+CGG + chr1_1:70184071-70184090 Msa0040150:CDS 45.0%
ATGATTAAACCGGGGTGAAC+CGG - chr1_1:70184105-70184124 None:intergenic 45.0%
! ACATTTTCCCTGTTCGGTTC+TGG - chr1_1:70184277-70184296 None:intergenic 45.0%
! AAGATGTTTGGGACTTGGGT+TGG + chr1_1:70184485-70184504 Msa0040150:three_prime_UTR 45.0%
! AGATGTTTGGGACTTGGGTT+GGG + chr1_1:70184486-70184505 Msa0040150:three_prime_UTR 45.0%
GGTTCTCTGTTGTGTAGAGA+GGG - chr1_1:70184706-70184725 None:intergenic 45.0%
TGGTTCTCTGTTGTGTAGAG+AGG - chr1_1:70184707-70184726 None:intergenic 45.0%
! AAGAGAGAATGGAAGGTGTG+TGG - chr1_1:70183640-70183659 None:intergenic 45.0%
GTACGCACATATTGATGAGG+TGG - chr1_1:70183733-70183752 None:intergenic 45.0%
GCAGTACGCACATATTGATG+AGG - chr1_1:70183736-70183755 None:intergenic 45.0%
!! AGCAATGGTGGTGGTGTTTA+GGG - chr1_1:70183763-70183782 None:intergenic 45.0%
!! AAGCAATGGTGGTGGTGTTT+AGG - chr1_1:70183764-70183783 None:intergenic 45.0%
ATAACCAGAAGCAATGGTGG+TGG - chr1_1:70183772-70183791 None:intergenic 45.0%
TTGTGGTGCGAGAAGAGTTA+TGG - chr1_1:70183834-70183853 None:intergenic 45.0%
TCACACTTGGATCTACACGA+CGG - chr1_1:70184051-70184070 None:intergenic 45.0%
CGTGTAGATCCAAGTGTGAT+TGG + chr1_1:70184052-70184071 Msa0040150:CDS 45.0%
TGGGTTACACCAATCACACT+TGG - chr1_1:70184064-70184083 None:intergenic 45.0%
TTGGTGTAACCCATGTCAAC+CGG + chr1_1:70184071-70184090 Msa0040150:CDS 45.0%
ATGATTAAACCGGGGTGAAC+CGG - chr1_1:70184105-70184124 None:intergenic 45.0%
! ACATTTTCCCTGTTCGGTTC+TGG - chr1_1:70184277-70184296 None:intergenic 45.0%
! AAGATGTTTGGGACTTGGGT+TGG + chr1_1:70184485-70184504 Msa0040150:three_prime_UTR 45.0%
! AGATGTTTGGGACTTGGGTT+GGG + chr1_1:70184486-70184505 Msa0040150:three_prime_UTR 45.0%
GGTTCTCTGTTGTGTAGAGA+GGG - chr1_1:70184706-70184725 None:intergenic 45.0%
TGGTTCTCTGTTGTGTAGAG+AGG - chr1_1:70184707-70184726 None:intergenic 45.0%
CTCTCTCTCCTTCCCTCTTT+AGG + chr1_1:70183492-70183511 Msa0040150:five_prime_UTR 50.0%
!! AATGGTGGTGGTGTTTAGGG+TGG - chr1_1:70183760-70183779 None:intergenic 50.0%
AACACCACCACCATTGCTTC+TGG + chr1_1:70183765-70183784 Msa0040150:CDS 50.0%
CTCTTCTTCCACGGTGATGT+CGG - chr1_1:70183982-70184001 None:intergenic 50.0%
ATCACCGTGGAAGAAGAGAG+AGG + chr1_1:70183984-70184003 Msa0040150:CDS 50.0%
GAACGTGAACCGGTTGACAT+GGG - chr1_1:70184083-70184102 None:intergenic 50.0%
! ATGTCAACCGGTTCACGTTC+CGG + chr1_1:70184083-70184102 Msa0040150:CDS 50.0%
AAGCATGGCGTTGCAAGTGT+GGG + chr1_1:70184145-70184164 Msa0040150:CDS 50.0%
AAACGTTGCCAGAACCGAAC+AGG + chr1_1:70184266-70184285 Msa0040150:three_prime_UTR 50.0%
AACGTTGCCAGAACCGAACA+GGG + chr1_1:70184267-70184286 Msa0040150:three_prime_UTR 50.0%
!! TTTGGGACTTGGGTTGGGTT+TGG + chr1_1:70184491-70184510 Msa0040150:three_prime_UTR 50.0%
CTCTCTCTCCTTCCCTCTTT+AGG + chr1_1:70183492-70183511 Msa0040150:five_prime_UTR 50.0%
!! AATGGTGGTGGTGTTTAGGG+TGG - chr1_1:70183760-70183779 None:intergenic 50.0%
AACACCACCACCATTGCTTC+TGG + chr1_1:70183765-70183784 Msa0040150:CDS 50.0%
CTCTTCTTCCACGGTGATGT+CGG - chr1_1:70183982-70184001 None:intergenic 50.0%
ATCACCGTGGAAGAAGAGAG+AGG + chr1_1:70183984-70184003 Msa0040150:CDS 50.0%
GAACGTGAACCGGTTGACAT+GGG - chr1_1:70184083-70184102 None:intergenic 50.0%
! ATGTCAACCGGTTCACGTTC+CGG + chr1_1:70184083-70184102 Msa0040150:CDS 50.0%
AAGCATGGCGTTGCAAGTGT+GGG + chr1_1:70184145-70184164 Msa0040150:CDS 50.0%
AAACGTTGCCAGAACCGAAC+AGG + chr1_1:70184266-70184285 Msa0040150:three_prime_UTR 50.0%
AACGTTGCCAGAACCGAACA+GGG + chr1_1:70184267-70184286 Msa0040150:three_prime_UTR 50.0%
!! TTTGGGACTTGGGTTGGGTT+TGG + chr1_1:70184491-70184510 Msa0040150:three_prime_UTR 50.0%
ACCGTGGAAGAAGAGAGAGG+TGG + chr1_1:70183987-70184006 Msa0040150:CDS 55.0%
GCCACCTCTCTCTTCTTCCA+CGG - chr1_1:70183991-70184010 None:intergenic 55.0%
GTGGAAGAAGAGAGAGGTGG+CGG + chr1_1:70183990-70184009 Msa0040150:CDS 55.0%
CGGAGCAGAAGAAGGTTGCT+GGG + chr1_1:70184010-70184029 Msa0040150:CDS 55.0%
AGAAGAAGGTTGCTGGGCCA+GGG + chr1_1:70184016-70184035 Msa0040150:CDS 55.0%
CACTTGGATCTACACGACGG+AGG - chr1_1:70184048-70184067 None:intergenic 55.0%
GGAACGTGAACCGGTTGACA+TGG - chr1_1:70184084-70184103 None:intergenic 55.0%
CACTTGCAACGCCATGCTTC+TGG - chr1_1:70184144-70184163 None:intergenic 55.0%
GAAGCATGGCGTTGCAAGTG+TGG + chr1_1:70184144-70184163 Msa0040150:CDS 55.0%
ACCGTGGAAGAAGAGAGAGG+TGG + chr1_1:70183987-70184006 Msa0040150:CDS 55.0%
GCCACCTCTCTCTTCTTCCA+CGG - chr1_1:70183991-70184010 None:intergenic 55.0%
GTGGAAGAAGAGAGAGGTGG+CGG + chr1_1:70183990-70184009 Msa0040150:CDS 55.0%
CGGAGCAGAAGAAGGTTGCT+GGG + chr1_1:70184010-70184029 Msa0040150:CDS 55.0%
AGAAGAAGGTTGCTGGGCCA+GGG + chr1_1:70184016-70184035 Msa0040150:CDS 55.0%
CACTTGGATCTACACGACGG+AGG - chr1_1:70184048-70184067 None:intergenic 55.0%
GGAACGTGAACCGGTTGACA+TGG - chr1_1:70184084-70184103 None:intergenic 55.0%
CACTTGCAACGCCATGCTTC+TGG - chr1_1:70184144-70184163 None:intergenic 55.0%
GAAGCATGGCGTTGCAAGTG+TGG + chr1_1:70184144-70184163 Msa0040150:CDS 55.0%
!! AGAGGTGGCGGAGCAGAAGA+AGG + chr1_1:70184002-70184021 Msa0040150:CDS 60.0%
GCGGAGCAGAAGAAGGTTGC+TGG + chr1_1:70184009-70184028 Msa0040150:CDS 60.0%
CAGAAGAAGGTTGCTGGGCC+AGG + chr1_1:70184015-70184034 Msa0040150:CDS 60.0%
GGGTGAACCGGAACGTGAAC+CGG - chr1_1:70184093-70184112 None:intergenic 60.0%
GTTCACGTTCCGGTTCACCC+CGG + chr1_1:70184093-70184112 Msa0040150:CDS 60.0%
!! AGAGGTGGCGGAGCAGAAGA+AGG + chr1_1:70184002-70184021 Msa0040150:CDS 60.0%
GCGGAGCAGAAGAAGGTTGC+TGG + chr1_1:70184009-70184028 Msa0040150:CDS 60.0%
CAGAAGAAGGTTGCTGGGCC+AGG + chr1_1:70184015-70184034 Msa0040150:CDS 60.0%
GGGTGAACCGGAACGTGAAC+CGG - chr1_1:70184093-70184112 None:intergenic 60.0%
GTTCACGTTCCGGTTCACCC+CGG + chr1_1:70184093-70184112 Msa0040150:CDS 60.0%
ACGGGTCTCCGACATCACCG+TGG + chr1_1:70183971-70183990 Msa0040150:CDS 65.0%
!! CACGACGGAGGTGTTGACCC+TGG - chr1_1:70184036-70184055 None:intergenic 65.0%
ACGGGTCTCCGACATCACCG+TGG + chr1_1:70183971-70183990 Msa0040150:CDS 65.0%
!! CACGACGGAGGTGTTGACCC+TGG - chr1_1:70184036-70184055 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 70183468 70184740 70183468 ID=Msa0040150;Name=Msa0040150
chr1_1 mRNA 70183468 70184740 70183468 ID=Msa0040150-mRNA-1;Parent=Msa0040150;Name=Msa0040150-mRNA-1;_AED=0.00;_eAED=0.00;_QI=275|1|1|1|1|1|2|552|122
chr1_1 exon 70183468 70183857 70183468 ID=Msa0040150-mRNA-1:exon:23047;Parent=Msa0040150-mRNA-1
chr1_1 exon 70183935 70184740 70183935 ID=Msa0040150-mRNA-1:exon:23048;Parent=Msa0040150-mRNA-1
chr1_1 five_prime_UTR 70183468 70183742 70183468 ID=Msa0040150-mRNA-1:five_prime_utr;Parent=Msa0040150-mRNA-1
chr1_1 CDS 70183743 70183857 70183743 ID=Msa0040150-mRNA-1:cds;Parent=Msa0040150-mRNA-1
chr1_1 CDS 70183935 70184188 70183935 ID=Msa0040150-mRNA-1:cds;Parent=Msa0040150-mRNA-1
chr1_1 three_prime_UTR 70184189 70184740 70184189 ID=Msa0040150-mRNA-1:three_prime_utr;Parent=Msa0040150-mRNA-1
Gene Sequence

>Msa0040150

ATGTGCGTACTGCGCCACCCTAAACACCACCACCATTGCTTCTGGTTATTACCCACCTTCATTTTTCTCTTGTTTTCTTCTTCTTCAGCCATAACTCTTCTCGCACCACAACCTAGTAGTAGTGGAACAGTGAAGCATCAAGAAATGGAAAACGGGTCTCCGACATCACCGTGGAAGAAGAGAGAGGTGGCGGAGCAGAAGAAGGTTGCTGGGCCAGGGTCAACACCTCCGTCGTGTAGATCCAAGTGTGATTGGTGTAACCCATGTCAACCGGTTCACGTTCCGGTTCACCCCGGTTTAATCATACGTCTTGAGTATTATCCAGAAGCATGGCGTTGCAAGTGTGGGAATAAGTATTTTTTGCCTTGA
Protein sequence

>Msa0040150

MCVLRHPKHHHHCFWLLPTFIFLLFSSSSAITLLAPQPSSSGTVKHQEMENGSPTSPWKKREVAEQKKVAGPGSTPPSCRSKCDWCNPCQPVHVPVHPGLIIRLEYYPEAWRCKCGNKYFLP*