AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0180490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0180490 MtrunA17_Chr1g0204791 91.057 123 9 2 1 122 1 122 8.53e-77 223
Msa0180490 MtrunA17_Chr5g0432501 37.069 116 66 2 14 122 21 136 2.59e-21 82.8
Msa0180490 MtrunA17_Chr8g0342581 35.200 125 58 4 20 122 6 129 8.07e-15 65.9
Msa0180490 MtrunA17_Chr4g0019101 42.105 76 39 2 47 122 92 162 1.22e-13 63.5
Msa0180490 MtrunA17_Chr4g0019111 42.188 64 35 1 59 122 16 77 7.78e-11 54.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0180490 AT3G22820.1 46.729 107 54 1 16 122 4 107 1.15e-27 98.2
Msa0180490 AT4G14723.1 49.524 105 40 3 19 122 17 109 6.24e-27 96.7
Msa0180490 AT2G30370.2 44.828 116 51 4 16 122 45 156 7.87e-23 87.4
Msa0180490 AT2G30370.3 44.828 116 51 4 16 122 55 166 1.02e-22 87.4
Msa0180490 AT2G30370.1 51.190 84 33 2 47 122 147 230 1.06e-21 86.3

Find 75 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCTCTCCTTCCCTCTTT+AGG 0.211079 1_4:-68454196 Msa0180490:five_prime_UTR
TTGTAATTATATGAATTATT+TGG 0.238057 1_4:-68453012 Msa0180490:three_prime_UTR
CACTTGCAACGCCATGCTTC+TGG 0.251321 1_4:+68453547 None:intergenic
TGCTCTGAGGTTTGAATTGT+TGG 0.274165 1_4:-68453307 Msa0180490:three_prime_UTR
TTTGGGACTTGGGTTGGGTT+TGG 0.275308 1_4:-68453198 Msa0180490:three_prime_UTR
CATTGGGAATGTAAGATGTT+TGG 0.278233 1_4:-68453216 Msa0180490:three_prime_UTR
CCAATGAAGAAGCCTTAAAA+AGG 0.292236 1_4:+68453233 None:intergenic
AAGCAATGGTGGTGGTGTTT+AGG 0.302399 1_4:+68453927 None:intergenic
ATTGGGAATGTAAGATGTTT+GGG 0.318124 1_4:-68453215 Msa0180490:three_prime_UTR
AACACCACCACCATTGCTTC+TGG 0.327365 1_4:-68453923 Msa0180490:CDS
AGCAATGGTGGTGGTGTTTA+GGG 0.333049 1_4:+68453928 None:intergenic
ATGATTAAACCGGGGTGAAC+CGG 0.350078 1_4:+68453586 None:intergenic
AGATGTTTGGGACTTGGGTT+GGG 0.351631 1_4:-68453203 Msa0180490:three_prime_UTR
GCGGAGCAGAAGAAGGTTGC+TGG 0.381489 1_4:-68453679 Msa0180490:CDS
CAGAAGAAGGTTGCTGGGCC+AGG 0.385682 1_4:-68453673 Msa0180490:CDS
AAACGTTGCCAGAACCGAAC+AGG 0.401440 1_4:-68453423 Msa0180490:three_prime_UTR
ATGTAAGATGTTTGGGACTT+GGG 0.402279 1_4:-68453208 Msa0180490:three_prime_UTR
CTCAAGACGTATGATTAAAC+CGG 0.405928 1_4:+68453576 None:intergenic
GAGAAGAAATGAACCTAAAG+AGG 0.416992 1_4:+68454183 None:intergenic
GGGTGAACCGGAACGTGAAC+CGG 0.417630 1_4:+68453598 None:intergenic
AAGATGTTTGGGACTTGGGT+TGG 0.421077 1_4:-68453204 Msa0180490:three_prime_UTR
AATGTAAGATGTTTGGGACT+TGG 0.424168 1_4:-68453209 Msa0180490:three_prime_UTR
GAGGTTTGAATTGTTGGACT+TGG 0.433808 1_4:-68453301 Msa0180490:three_prime_UTR
TGAGAGTGAGAAAGAGAGAA+TGG 0.434630 1_4:+68454040 None:intergenic
ATGTCAACCGGTTCACGTTC+CGG 0.448654 1_4:-68453605 Msa0180490:CDS
AACAGTGAAGCATCAAGAAA+TGG 0.452869 1_4:-68453743 Msa0180490:CDS
GTTTGAATTGTTGGACTTGG+TGG 0.458192 1_4:-68453298 Msa0180490:three_prime_UTR
AAGCATCAAGAAATGGAAAA+CGG 0.474883 1_4:-68453736 Msa0180490:CDS
CTCTTCTTCCACGGTGATGT+CGG 0.479412 1_4:+68453709 None:intergenic
AATGACAAAATACTATGTTA+AGG 0.481772 1_4:-68453049 Msa0180490:three_prime_UTR
AGCATCAAGAAATGGAAAAC+GGG 0.486636 1_4:-68453735 Msa0180490:CDS
CGTGTAGATCCAAGTGTGAT+TGG 0.513041 1_4:-68453636 Msa0180490:CDS
GAAGCATGGCGTTGCAAGTG+TGG 0.519320 1_4:-68453544 Msa0180490:CDS
AGAGGTGGCGGAGCAGAAGA+AGG 0.519433 1_4:-68453686 Msa0180490:CDS
TTGGTGTAACCCATGTCAAC+CGG 0.519681 1_4:-68453617 Msa0180490:CDS
GGAACGTGAACCGGTTGACA+TGG 0.526123 1_4:+68453607 None:intergenic
AGTGAGAAAGAGAGAATGGA+AGG 0.528050 1_4:+68454044 None:intergenic
TACTTATCAATTCATCATCA+AGG 0.529658 1_4:+68453505 None:intergenic
GCCACCTCTCTCTTCTTCCA+CGG 0.532769 1_4:+68453700 None:intergenic
CGGAGCAGAAGAAGGTTGCT+GGG 0.544379 1_4:-68453678 Msa0180490:CDS
GTATTATTAAATGTTACTGC+AGG 0.545034 1_4:+68454073 None:intergenic
GTGGAAGAAGAGAGAGGTGG+CGG 0.545993 1_4:-68453698 Msa0180490:CDS
TTGTGGTGCGAGAAGAGTTA+TGG 0.548135 1_4:+68453857 None:intergenic
AGAGTACAGAGAAGATGAAG+AGG 0.551498 1_4:+68454009 None:intergenic
AACTTGAACTTACTAGGTTG+TGG 0.559169 1_4:+68453840 None:intergenic
AGAAGAAGGTTGCTGGGCCA+GGG 0.561033 1_4:-68453672 Msa0180490:CDS
AAGAGAGAATGGAAGGTGTG+TGG 0.576934 1_4:+68454051 None:intergenic
GGTTCTCTGTTGTGTAGAGA+GGG 0.587339 1_4:+68452986 None:intergenic
TGGTTCTCTGTTGTGTAGAG+AGG 0.588574 1_4:+68452985 None:intergenic
GAAATGAACCTAAAGAGGGA+AGG 0.600783 1_4:+68454188 None:intergenic
TCAAGACGTATGATTAAACC+GGG 0.608793 1_4:+68453577 None:intergenic
TGGGTTACACCAATCACACT+TGG 0.609884 1_4:+68453627 None:intergenic
AAGCATGGCGTTGCAAGTGT+GGG 0.610762 1_4:-68453543 Msa0180490:CDS
AGAAGAAATGAACCTAAAGA+GGG 0.612120 1_4:+68454184 None:intergenic
CACGACGGAGGTGTTGACCC+TGG 0.613267 1_4:+68453655 None:intergenic
GAACGTGAACCGGTTGACAT+GGG 0.614728 1_4:+68453608 None:intergenic
GTACGCACATATTGATGAGG+TGG 0.617360 1_4:+68453958 None:intergenic
ATAGAAGAAGAATAACAAGA+TGG 0.617872 1_4:+68452965 None:intergenic
TTGAGTATTATCCAGAAGCA+TGG 0.618402 1_4:-68453558 Msa0180490:CDS
TACTACTATGTACAATAGAG+AGG 0.621909 1_4:+68453146 None:intergenic
AACGTTGCCAGAACCGAACA+GGG 0.633112 1_4:-68453422 Msa0180490:three_prime_UTR
TGGGTAATAACCAGAAGCAA+TGG 0.633930 1_4:+68453913 None:intergenic
GTTCACGTTCCGGTTCACCC+CGG 0.635691 1_4:-68453595 Msa0180490:CDS
ATAACCAGAAGCAATGGTGG+TGG 0.639918 1_4:+68453919 None:intergenic
GTAATAACCAGAAGCAATGG+TGG 0.650622 1_4:+68453916 None:intergenic
AATGGTGGTGGTGTTTAGGG+TGG 0.653961 1_4:+68453931 None:intergenic
ACTACTATGTACAATAGAGA+GGG 0.659336 1_4:+68453147 None:intergenic
GCAGTACGCACATATTGATG+AGG 0.669919 1_4:+68453955 None:intergenic
GTATTAATGATGTTGCTCTG+AGG 0.673923 1_4:-68453320 Msa0180490:three_prime_UTR
ACCGTGGAAGAAGAGAGAGG+TGG 0.701228 1_4:-68453701 Msa0180490:CDS
ATCACCGTGGAAGAAGAGAG+AGG 0.712922 1_4:-68453704 Msa0180490:CDS
CACTTGGATCTACACGACGG+AGG 0.750576 1_4:+68453643 None:intergenic
TCACACTTGGATCTACACGA+CGG 0.754283 1_4:+68453640 None:intergenic
ACGGGTCTCCGACATCACCG+TGG 0.754489 1_4:-68453717 Msa0180490:CDS
CAAGACGTATGATTAAACCG+GGG 0.816477 1_4:+68453578 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTACAATAAAAACATTAAA+AGG + chr1_4:68454120-68454139 None:intergenic 10.0%
!! TTGTAATTATATGAATTATT+TGG - chr1_4:68454179-68454198 Msa0180490:five_prime_UTR 10.0%
!! TTTACAATAAAAACATTAAA+AGG + chr1_4:68454120-68454139 None:intergenic 10.0%
!! TTGTAATTATATGAATTATT+TGG - chr1_4:68454179-68454198 Msa0180490:five_prime_UTR 10.0%
!!! TGAAAAAAGTATGAAGAAAA+TGG + chr1_4:68453392-68453411 None:intergenic 20.0%
!!! TGAGTACTAATATTGATTTT+TGG - chr1_4:68453930-68453949 Msa0180490:CDS 20.0%
!!! TTTTTGGTTTAACCTTTTTA+AGG - chr1_4:68453946-68453965 Msa0180490:CDS 20.0%
!!! TCTATTTCCTTTTTTGTTTT+AGG - chr1_4:68454075-68454094 Msa0180490:five_prime_UTR 20.0%
!! ACATAAACCTAAAACAAAAA+AGG + chr1_4:68454085-68454104 None:intergenic 20.0%
!! ACAATAAAAACATTAAAAGG+AGG + chr1_4:68454117-68454136 None:intergenic 20.0%
!! AATGACAAAATACTATGTTA+AGG - chr1_4:68454142-68454161 Msa0180490:five_prime_UTR 20.0%
!!! TGAAAAAAGTATGAAGAAAA+TGG + chr1_4:68453392-68453411 None:intergenic 20.0%
!!! TGAGTACTAATATTGATTTT+TGG - chr1_4:68453930-68453949 Msa0180490:CDS 20.0%
!!! TTTTTGGTTTAACCTTTTTA+AGG - chr1_4:68453946-68453965 Msa0180490:CDS 20.0%
!!! TCTATTTCCTTTTTTGTTTT+AGG - chr1_4:68454075-68454094 Msa0180490:five_prime_UTR 20.0%
!! ACATAAACCTAAAACAAAAA+AGG + chr1_4:68454085-68454104 None:intergenic 20.0%
!! ACAATAAAAACATTAAAAGG+AGG + chr1_4:68454117-68454136 None:intergenic 20.0%
!! AATGACAAAATACTATGTTA+AGG - chr1_4:68454142-68454161 Msa0180490:five_prime_UTR 20.0%
! GTATTATTAAATGTTACTGC+AGG + chr1_4:68453121-68453140 None:intergenic 25.0%
! AGAAAACAAGAGAAAAATGA+AGG + chr1_4:68453304-68453323 None:intergenic 25.0%
! TACTTATCAATTCATCATCA+AGG + chr1_4:68453689-68453708 None:intergenic 25.0%
!!! TGTGTTTTTTCTTTTGTACT+AGG + chr1_4:68453749-68453768 None:intergenic 25.0%
! AAAAGGGGAAAAATTTAAGA+TGG + chr1_4:68453812-68453831 None:intergenic 25.0%
!! ATCTTAAATTTTTCCCCTTT+TGG - chr1_4:68453811-68453830 Msa0180490:intron 25.0%
!! TCTTAAATTTTTCCCCTTTT+GGG - chr1_4:68453812-68453831 Msa0180490:intron 25.0%
! TAATACAAAAAAGCCCAAAA+GGG + chr1_4:68453828-68453847 None:intergenic 25.0%
! ATAATACAAAAAAGCCCAAA+AGG + chr1_4:68453829-68453848 None:intergenic 25.0%
! GTATTATTAAATGTTACTGC+AGG + chr1_4:68453121-68453140 None:intergenic 25.0%
! AGAAAACAAGAGAAAAATGA+AGG + chr1_4:68453304-68453323 None:intergenic 25.0%
! TACTTATCAATTCATCATCA+AGG + chr1_4:68453689-68453708 None:intergenic 25.0%
!!! TGTGTTTTTTCTTTTGTACT+AGG + chr1_4:68453749-68453768 None:intergenic 25.0%
! AAAAGGGGAAAAATTTAAGA+TGG + chr1_4:68453812-68453831 None:intergenic 25.0%
!! ATCTTAAATTTTTCCCCTTT+TGG - chr1_4:68453811-68453830 Msa0180490:intron 25.0%
!! TCTTAAATTTTTCCCCTTTT+GGG - chr1_4:68453812-68453831 Msa0180490:intron 25.0%
! TAATACAAAAAAGCCCAAAA+GGG + chr1_4:68453828-68453847 None:intergenic 25.0%
! ATAATACAAAAAAGCCCAAA+AGG + chr1_4:68453829-68453848 None:intergenic 25.0%
AGAAGAAATGAACCTAAAGA+GGG + chr1_4:68453010-68453029 None:intergenic 30.0%
!! TCATTTCTTCTCTTTTGACT+TGG - chr1_4:68453019-68453038 Msa0180490:three_prime_UTR 30.0%
AACAAGAGAAAAATGAAGGT+GGG + chr1_4:68453300-68453319 None:intergenic 30.0%
AAACAAGAGAAAAATGAAGG+TGG + chr1_4:68453301-68453320 None:intergenic 30.0%
GAAATGAACTTGAACTTACT+AGG + chr1_4:68453360-68453379 None:intergenic 30.0%
! CAACAACTCTTTTCATTTTC+AGG - chr1_4:68453416-68453435 Msa0180490:three_prime_UTR 30.0%
!!! TTTTCATTTTCAGGTAGTAG+TGG - chr1_4:68453425-68453444 Msa0180490:three_prime_UTR 30.0%
AAGCATCAAGAAATGGAAAA+CGG - chr1_4:68453455-68453474 Msa0180490:three_prime_UTR 30.0%
AATACAAAAAAGCCCAAAAG+GGG + chr1_4:68453827-68453846 None:intergenic 30.0%
!!! CTTTTTAAGGCTTCTTCATT+GGG - chr1_4:68453959-68453978 Msa0180490:exon 30.0%
ATTGGGAATGTAAGATGTTT+GGG - chr1_4:68453976-68453995 Msa0180490:five_prime_UTR 30.0%
ACTACTATGTACAATAGAGA+GGG + chr1_4:68454047-68454066 None:intergenic 30.0%
TACTACTATGTACAATAGAG+AGG + chr1_4:68454048-68454067 None:intergenic 30.0%
! ATATCTTGTTTGAGTATCTG+AGG + chr1_4:68452973-68452992 None:intergenic 30.0%
AGAAGAAATGAACCTAAAGA+GGG + chr1_4:68453010-68453029 None:intergenic 30.0%
!! TCATTTCTTCTCTTTTGACT+TGG - chr1_4:68453019-68453038 Msa0180490:three_prime_UTR 30.0%
AACAAGAGAAAAATGAAGGT+GGG + chr1_4:68453300-68453319 None:intergenic 30.0%
AAACAAGAGAAAAATGAAGG+TGG + chr1_4:68453301-68453320 None:intergenic 30.0%
GAAATGAACTTGAACTTACT+AGG + chr1_4:68453360-68453379 None:intergenic 30.0%
! CAACAACTCTTTTCATTTTC+AGG - chr1_4:68453416-68453435 Msa0180490:three_prime_UTR 30.0%
!!! TTTTCATTTTCAGGTAGTAG+TGG - chr1_4:68453425-68453444 Msa0180490:three_prime_UTR 30.0%
AAGCATCAAGAAATGGAAAA+CGG - chr1_4:68453455-68453474 Msa0180490:three_prime_UTR 30.0%
AATACAAAAAAGCCCAAAAG+GGG + chr1_4:68453827-68453846 None:intergenic 30.0%
!!! CTTTTTAAGGCTTCTTCATT+GGG - chr1_4:68453959-68453978 Msa0180490:exon 30.0%
ATTGGGAATGTAAGATGTTT+GGG - chr1_4:68453976-68453995 Msa0180490:five_prime_UTR 30.0%
ACTACTATGTACAATAGAGA+GGG + chr1_4:68454047-68454066 None:intergenic 30.0%
TACTACTATGTACAATAGAG+AGG + chr1_4:68454048-68454067 None:intergenic 30.0%
GAGAAGAAATGAACCTAAAG+AGG + chr1_4:68453011-68453030 None:intergenic 35.0%
AACTTGAACTTACTAGGTTG+TGG + chr1_4:68453354-68453373 None:intergenic 35.0%
! AACAGTGAAGCATCAAGAAA+TGG - chr1_4:68453448-68453467 Msa0180490:three_prime_UTR 35.0%
AGCATCAAGAAATGGAAAAC+GGG - chr1_4:68453456-68453475 Msa0180490:three_prime_UTR 35.0%
TCAAGACGTATGATTAAACC+GGG + chr1_4:68453617-68453636 None:intergenic 35.0%
CTCAAGACGTATGATTAAAC+CGG + chr1_4:68453618-68453637 None:intergenic 35.0%
TTGAGTATTATCCAGAAGCA+TGG - chr1_4:68453633-68453652 Msa0180490:CDS 35.0%
GTATTAATGATGTTGCTCTG+AGG - chr1_4:68453871-68453890 Msa0180490:CDS 35.0%
CCAATGAAGAAGCCTTAAAA+AGG + chr1_4:68453961-68453980 None:intergenic 35.0%
!!! CCTTTTTAAGGCTTCTTCAT+TGG - chr1_4:68453958-68453977 Msa0180490:exon 35.0%
CATTGGGAATGTAAGATGTT+TGG - chr1_4:68453975-68453994 Msa0180490:five_prime_UTR 35.0%
! AATGTAAGATGTTTGGGACT+TGG - chr1_4:68453982-68454001 Msa0180490:five_prime_UTR 35.0%
! ATGTAAGATGTTTGGGACTT+GGG - chr1_4:68453983-68454002 Msa0180490:five_prime_UTR 35.0%
GAGAAGAAATGAACCTAAAG+AGG + chr1_4:68453011-68453030 None:intergenic 35.0%
AACTTGAACTTACTAGGTTG+TGG + chr1_4:68453354-68453373 None:intergenic 35.0%
! AACAGTGAAGCATCAAGAAA+TGG - chr1_4:68453448-68453467 Msa0180490:three_prime_UTR 35.0%
AGCATCAAGAAATGGAAAAC+GGG - chr1_4:68453456-68453475 Msa0180490:three_prime_UTR 35.0%
TCAAGACGTATGATTAAACC+GGG + chr1_4:68453617-68453636 None:intergenic 35.0%
CTCAAGACGTATGATTAAAC+CGG + chr1_4:68453618-68453637 None:intergenic 35.0%
TTGAGTATTATCCAGAAGCA+TGG - chr1_4:68453633-68453652 Msa0180490:CDS 35.0%
GTATTAATGATGTTGCTCTG+AGG - chr1_4:68453871-68453890 Msa0180490:CDS 35.0%
CCAATGAAGAAGCCTTAAAA+AGG + chr1_4:68453961-68453980 None:intergenic 35.0%
!!! CCTTTTTAAGGCTTCTTCAT+TGG - chr1_4:68453958-68453977 Msa0180490:exon 35.0%
CATTGGGAATGTAAGATGTT+TGG - chr1_4:68453975-68453994 Msa0180490:five_prime_UTR 35.0%
! AATGTAAGATGTTTGGGACT+TGG - chr1_4:68453982-68454001 Msa0180490:five_prime_UTR 35.0%
! ATGTAAGATGTTTGGGACTT+GGG - chr1_4:68453983-68454002 Msa0180490:five_prime_UTR 35.0%
GAAATGAACCTAAAGAGGGA+AGG + chr1_4:68453006-68453025 None:intergenic 40.0%
! CTCACATTTTCACAACACAC+AGG - chr1_4:68453063-68453082 Msa0180490:three_prime_UTR 40.0%
AGTGAGAAAGAGAGAATGGA+AGG + chr1_4:68453150-68453169 None:intergenic 40.0%
TGAGAGTGAGAAAGAGAGAA+TGG + chr1_4:68453154-68453173 None:intergenic 40.0%
AGAGTACAGAGAAGATGAAG+AGG + chr1_4:68453185-68453204 None:intergenic 40.0%
GTAATAACCAGAAGCAATGG+TGG + chr1_4:68453278-68453297 None:intergenic 40.0%
TGGGTAATAACCAGAAGCAA+TGG + chr1_4:68453281-68453300 None:intergenic 40.0%
CAAGACGTATGATTAAACCG+GGG + chr1_4:68453616-68453635 None:intergenic 40.0%
! TCTGCAACATTTTCCCTGTT+CGG + chr1_4:68453785-68453804 None:intergenic 40.0%
!! TGCTCTGAGGTTTGAATTGT+TGG - chr1_4:68453884-68453903 Msa0180490:CDS 40.0%
GAGGTTTGAATTGTTGGACT+TGG - chr1_4:68453890-68453909 Msa0180490:CDS 40.0%
GTTTGAATTGTTGGACTTGG+TGG - chr1_4:68453893-68453912 Msa0180490:CDS 40.0%
GAAATGAACCTAAAGAGGGA+AGG + chr1_4:68453006-68453025 None:intergenic 40.0%
! CTCACATTTTCACAACACAC+AGG - chr1_4:68453063-68453082 Msa0180490:three_prime_UTR 40.0%
AGTGAGAAAGAGAGAATGGA+AGG + chr1_4:68453150-68453169 None:intergenic 40.0%
TGAGAGTGAGAAAGAGAGAA+TGG + chr1_4:68453154-68453173 None:intergenic 40.0%
AGAGTACAGAGAAGATGAAG+AGG + chr1_4:68453185-68453204 None:intergenic 40.0%
GTAATAACCAGAAGCAATGG+TGG + chr1_4:68453278-68453297 None:intergenic 40.0%
TGGGTAATAACCAGAAGCAA+TGG + chr1_4:68453281-68453300 None:intergenic 40.0%
CAAGACGTATGATTAAACCG+GGG + chr1_4:68453616-68453635 None:intergenic 40.0%
! TCTGCAACATTTTCCCTGTT+CGG + chr1_4:68453785-68453804 None:intergenic 40.0%
!! TGCTCTGAGGTTTGAATTGT+TGG - chr1_4:68453884-68453903 Msa0180490:CDS 40.0%
GAGGTTTGAATTGTTGGACT+TGG - chr1_4:68453890-68453909 Msa0180490:CDS 40.0%
GTTTGAATTGTTGGACTTGG+TGG - chr1_4:68453893-68453912 Msa0180490:CDS 40.0%
! AAGAGAGAATGGAAGGTGTG+TGG + chr1_4:68453143-68453162 None:intergenic 45.0%
GTACGCACATATTGATGAGG+TGG + chr1_4:68453236-68453255 None:intergenic 45.0%
GCAGTACGCACATATTGATG+AGG + chr1_4:68453239-68453258 None:intergenic 45.0%
!! AGCAATGGTGGTGGTGTTTA+GGG + chr1_4:68453266-68453285 None:intergenic 45.0%
!! AAGCAATGGTGGTGGTGTTT+AGG + chr1_4:68453267-68453286 None:intergenic 45.0%
ATAACCAGAAGCAATGGTGG+TGG + chr1_4:68453275-68453294 None:intergenic 45.0%
TTGTGGTGCGAGAAGAGTTA+TGG + chr1_4:68453337-68453356 None:intergenic 45.0%
TCACACTTGGATCTACACGA+CGG + chr1_4:68453554-68453573 None:intergenic 45.0%
CGTGTAGATCCAAGTGTGAT+TGG - chr1_4:68453555-68453574 Msa0180490:CDS 45.0%
TGGGTTACACCAATCACACT+TGG + chr1_4:68453567-68453586 None:intergenic 45.0%
TTGGTGTAACCCATGTCAAC+CGG - chr1_4:68453574-68453593 Msa0180490:CDS 45.0%
ATGATTAAACCGGGGTGAAC+CGG + chr1_4:68453608-68453627 None:intergenic 45.0%
! ACATTTTCCCTGTTCGGTTC+TGG + chr1_4:68453779-68453798 None:intergenic 45.0%
! AAGATGTTTGGGACTTGGGT+TGG - chr1_4:68453987-68454006 Msa0180490:five_prime_UTR 45.0%
! AGATGTTTGGGACTTGGGTT+GGG - chr1_4:68453988-68454007 Msa0180490:five_prime_UTR 45.0%
GGTTCTCTGTTGTGTAGAGA+GGG + chr1_4:68454208-68454227 None:intergenic 45.0%
TGGTTCTCTGTTGTGTAGAG+AGG + chr1_4:68454209-68454228 None:intergenic 45.0%
! AAGAGAGAATGGAAGGTGTG+TGG + chr1_4:68453143-68453162 None:intergenic 45.0%
GTACGCACATATTGATGAGG+TGG + chr1_4:68453236-68453255 None:intergenic 45.0%
GCAGTACGCACATATTGATG+AGG + chr1_4:68453239-68453258 None:intergenic 45.0%
!! AGCAATGGTGGTGGTGTTTA+GGG + chr1_4:68453266-68453285 None:intergenic 45.0%
!! AAGCAATGGTGGTGGTGTTT+AGG + chr1_4:68453267-68453286 None:intergenic 45.0%
ATAACCAGAAGCAATGGTGG+TGG + chr1_4:68453275-68453294 None:intergenic 45.0%
TTGTGGTGCGAGAAGAGTTA+TGG + chr1_4:68453337-68453356 None:intergenic 45.0%
TCACACTTGGATCTACACGA+CGG + chr1_4:68453554-68453573 None:intergenic 45.0%
CGTGTAGATCCAAGTGTGAT+TGG - chr1_4:68453555-68453574 Msa0180490:CDS 45.0%
TGGGTTACACCAATCACACT+TGG + chr1_4:68453567-68453586 None:intergenic 45.0%
TTGGTGTAACCCATGTCAAC+CGG - chr1_4:68453574-68453593 Msa0180490:CDS 45.0%
ATGATTAAACCGGGGTGAAC+CGG + chr1_4:68453608-68453627 None:intergenic 45.0%
! ACATTTTCCCTGTTCGGTTC+TGG + chr1_4:68453779-68453798 None:intergenic 45.0%
! AAGATGTTTGGGACTTGGGT+TGG - chr1_4:68453987-68454006 Msa0180490:five_prime_UTR 45.0%
! AGATGTTTGGGACTTGGGTT+GGG - chr1_4:68453988-68454007 Msa0180490:five_prime_UTR 45.0%
GGTTCTCTGTTGTGTAGAGA+GGG + chr1_4:68454208-68454227 None:intergenic 45.0%
TGGTTCTCTGTTGTGTAGAG+AGG + chr1_4:68454209-68454228 None:intergenic 45.0%
CTCTCTCTCCTTCCCTCTTT+AGG - chr1_4:68452995-68453014 Msa0180490:three_prime_UTR 50.0%
!! AATGGTGGTGGTGTTTAGGG+TGG + chr1_4:68453263-68453282 None:intergenic 50.0%
AACACCACCACCATTGCTTC+TGG - chr1_4:68453268-68453287 Msa0180490:three_prime_UTR 50.0%
CTCTTCTTCCACGGTGATGT+CGG + chr1_4:68453485-68453504 None:intergenic 50.0%
ATCACCGTGGAAGAAGAGAG+AGG - chr1_4:68453487-68453506 Msa0180490:three_prime_UTR 50.0%
GAACGTGAACCGGTTGACAT+GGG + chr1_4:68453586-68453605 None:intergenic 50.0%
! ATGTCAACCGGTTCACGTTC+CGG - chr1_4:68453586-68453605 Msa0180490:CDS 50.0%
AAGCATGGCGTTGCAAGTGT+GGG - chr1_4:68453648-68453667 Msa0180490:CDS 50.0%
AAACGTTGCCAGAACCGAAC+AGG - chr1_4:68453768-68453787 Msa0180490:intron 50.0%
AACGTTGCCAGAACCGAACA+GGG - chr1_4:68453769-68453788 Msa0180490:intron 50.0%
!! TTTGGGACTTGGGTTGGGTT+TGG - chr1_4:68453993-68454012 Msa0180490:five_prime_UTR 50.0%
CTCTCTCTCCTTCCCTCTTT+AGG - chr1_4:68452995-68453014 Msa0180490:three_prime_UTR 50.0%
!! AATGGTGGTGGTGTTTAGGG+TGG + chr1_4:68453263-68453282 None:intergenic 50.0%
AACACCACCACCATTGCTTC+TGG - chr1_4:68453268-68453287 Msa0180490:three_prime_UTR 50.0%
CTCTTCTTCCACGGTGATGT+CGG + chr1_4:68453485-68453504 None:intergenic 50.0%
ATCACCGTGGAAGAAGAGAG+AGG - chr1_4:68453487-68453506 Msa0180490:three_prime_UTR 50.0%
GAACGTGAACCGGTTGACAT+GGG + chr1_4:68453586-68453605 None:intergenic 50.0%
! ATGTCAACCGGTTCACGTTC+CGG - chr1_4:68453586-68453605 Msa0180490:CDS 50.0%
AAGCATGGCGTTGCAAGTGT+GGG - chr1_4:68453648-68453667 Msa0180490:CDS 50.0%
AAACGTTGCCAGAACCGAAC+AGG - chr1_4:68453768-68453787 Msa0180490:intron 50.0%
AACGTTGCCAGAACCGAACA+GGG - chr1_4:68453769-68453788 Msa0180490:intron 50.0%
!! TTTGGGACTTGGGTTGGGTT+TGG - chr1_4:68453993-68454012 Msa0180490:five_prime_UTR 50.0%
ACCGTGGAAGAAGAGAGAGG+TGG - chr1_4:68453490-68453509 Msa0180490:three_prime_UTR 55.0%
GCCACCTCTCTCTTCTTCCA+CGG + chr1_4:68453494-68453513 None:intergenic 55.0%
GTGGAAGAAGAGAGAGGTGG+CGG - chr1_4:68453493-68453512 Msa0180490:three_prime_UTR 55.0%
CGGAGCAGAAGAAGGTTGCT+GGG - chr1_4:68453513-68453532 Msa0180490:exon 55.0%
AGAAGAAGGTTGCTGGGCCA+GGG - chr1_4:68453519-68453538 Msa0180490:exon 55.0%
CACTTGGATCTACACGACGG+AGG + chr1_4:68453551-68453570 None:intergenic 55.0%
GGAACGTGAACCGGTTGACA+TGG + chr1_4:68453587-68453606 None:intergenic 55.0%
CACTTGCAACGCCATGCTTC+TGG + chr1_4:68453647-68453666 None:intergenic 55.0%
GAAGCATGGCGTTGCAAGTG+TGG - chr1_4:68453647-68453666 Msa0180490:CDS 55.0%
ACCGTGGAAGAAGAGAGAGG+TGG - chr1_4:68453490-68453509 Msa0180490:three_prime_UTR 55.0%
GCCACCTCTCTCTTCTTCCA+CGG + chr1_4:68453494-68453513 None:intergenic 55.0%
GTGGAAGAAGAGAGAGGTGG+CGG - chr1_4:68453493-68453512 Msa0180490:three_prime_UTR 55.0%
CGGAGCAGAAGAAGGTTGCT+GGG - chr1_4:68453513-68453532 Msa0180490:exon 55.0%
AGAAGAAGGTTGCTGGGCCA+GGG - chr1_4:68453519-68453538 Msa0180490:exon 55.0%
CACTTGGATCTACACGACGG+AGG + chr1_4:68453551-68453570 None:intergenic 55.0%
GGAACGTGAACCGGTTGACA+TGG + chr1_4:68453587-68453606 None:intergenic 55.0%
CACTTGCAACGCCATGCTTC+TGG + chr1_4:68453647-68453666 None:intergenic 55.0%
GAAGCATGGCGTTGCAAGTG+TGG - chr1_4:68453647-68453666 Msa0180490:CDS 55.0%
!! AGAGGTGGCGGAGCAGAAGA+AGG - chr1_4:68453505-68453524 Msa0180490:exon 60.0%
GCGGAGCAGAAGAAGGTTGC+TGG - chr1_4:68453512-68453531 Msa0180490:exon 60.0%
CAGAAGAAGGTTGCTGGGCC+AGG - chr1_4:68453518-68453537 Msa0180490:exon 60.0%
GGGTGAACCGGAACGTGAAC+CGG + chr1_4:68453596-68453615 None:intergenic 60.0%
GTTCACGTTCCGGTTCACCC+CGG - chr1_4:68453596-68453615 Msa0180490:CDS 60.0%
!! AGAGGTGGCGGAGCAGAAGA+AGG - chr1_4:68453505-68453524 Msa0180490:exon 60.0%
GCGGAGCAGAAGAAGGTTGC+TGG - chr1_4:68453512-68453531 Msa0180490:exon 60.0%
CAGAAGAAGGTTGCTGGGCC+AGG - chr1_4:68453518-68453537 Msa0180490:exon 60.0%
GGGTGAACCGGAACGTGAAC+CGG + chr1_4:68453596-68453615 None:intergenic 60.0%
GTTCACGTTCCGGTTCACCC+CGG - chr1_4:68453596-68453615 Msa0180490:CDS 60.0%
ACGGGTCTCCGACATCACCG+TGG - chr1_4:68453474-68453493 Msa0180490:three_prime_UTR 65.0%
!! CACGACGGAGGTGTTGACCC+TGG + chr1_4:68453539-68453558 None:intergenic 65.0%
ACGGGTCTCCGACATCACCG+TGG - chr1_4:68453474-68453493 Msa0180490:three_prime_UTR 65.0%
!! CACGACGGAGGTGTTGACCC+TGG + chr1_4:68453539-68453558 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 68452971 68454242 68452971 ID=Msa0180490;Name=Msa0180490
chr1_4 mRNA 68452971 68454242 68452971 ID=Msa0180490-mRNA-1;Parent=Msa0180490;Name=Msa0180490-mRNA-1;_AED=0.00;_eAED=0.00;_QI=275|1|1|1|1|1|2|551|122
chr1_4 exon 68452971 68453775 68452971 ID=Msa0180490-mRNA-1:exon:22591;Parent=Msa0180490-mRNA-1
chr1_4 exon 68453853 68454242 68453853 ID=Msa0180490-mRNA-1:exon:22590;Parent=Msa0180490-mRNA-1
chr1_4 five_prime_UTR 68453968 68454242 68453968 ID=Msa0180490-mRNA-1:five_prime_utr;Parent=Msa0180490-mRNA-1
chr1_4 CDS 68453853 68453967 68453853 ID=Msa0180490-mRNA-1:cds;Parent=Msa0180490-mRNA-1
chr1_4 CDS 68453522 68453775 68453522 ID=Msa0180490-mRNA-1:cds;Parent=Msa0180490-mRNA-1
chr1_4 three_prime_UTR 68452971 68453521 68452971 ID=Msa0180490-mRNA-1:three_prime_utr;Parent=Msa0180490-mRNA-1
Gene Sequence

>Msa0180490

ATGTGCGTACTGCGCCACCCTAAACACCACCACCATTGCTTCTGGTTATTACCCACCTTCATTTTTCTCTTGTTTTCTTCTTCTTCAGCCATAACTCTTCTCGCACCACAACCTAGTAGTAGTGGAACAGTGAAGCATCAAGAAATGGAAAACGGGTCTCCGACATCACCGTGGAAGAAGAGAGAGGTGGCGGAGCAGAAGAAGGTTGCTGGGCCAGGGTCAACACCTCCGTCGTGTAGATCCAAGTGTGATTGGTGTAACCCATGTCAACCGGTTCACGTTCCGGTTCACCCCGGTTTAATCATACGTCTTGAGTATTATCCAGAAGCATGGCGTTGCAAGTGTGGGAATAAGTATTTTTTGCCTTGA
Protein sequence

>Msa0180490

MCVLRHPKHHHHCFWLLPTFIFLLFSSSSAITLLAPQPSSSGTVKHQEMENGSPTSPWKKREVAEQKKVAGPGSTPPSCRSKCDWCNPCQPVHVPVHPGLIIRLEYYPEAWRCKCGNKYFLP*