AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0868060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0868060 MtrunA17_Chr1g0204791 91.057 123 9 2 1 122 1 122 8.53e-77 223
Msa0868060 MtrunA17_Chr5g0432501 37.069 116 66 2 14 122 21 136 2.59e-21 82.8
Msa0868060 MtrunA17_Chr8g0342581 35.200 125 58 4 20 122 6 129 8.07e-15 65.9
Msa0868060 MtrunA17_Chr4g0019101 42.105 76 39 2 47 122 92 162 1.22e-13 63.5
Msa0868060 MtrunA17_Chr4g0019111 42.188 64 35 1 59 122 16 77 7.78e-11 54.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0868060 AT3G22820.1 46.729 107 54 1 16 122 4 107 1.15e-27 98.2
Msa0868060 AT4G14723.1 49.524 105 40 3 19 122 17 109 6.24e-27 96.7
Msa0868060 AT2G30370.2 44.828 116 51 4 16 122 45 156 7.87e-23 87.4
Msa0868060 AT2G30370.3 44.828 116 51 4 16 122 55 166 1.02e-22 87.4
Msa0868060 AT2G30370.1 51.190 84 33 2 47 122 147 230 1.06e-21 86.3

Find 75 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCTCTCCTTCCCTCTTT+AGG 0.211079 6_1:+1519103 Msa0868060:five_prime_UTR
TTGTAATTATATGAATTATT+TGG 0.238057 6_1:+1520279 Msa0868060:three_prime_UTR
CACTTGCAACGCCATGCTTC+TGG 0.251321 6_1:-1519749 None:intergenic
TGCTCTGAGGTTTGAATTGT+TGG 0.274165 6_1:+1519989 Msa0868060:three_prime_UTR
TTTGGGACTTGGGTTGGGTT+TGG 0.275308 6_1:+1520098 Msa0868060:three_prime_UTR
CATTGGGAATGTAAGATGTT+TGG 0.278233 6_1:+1520080 Msa0868060:three_prime_UTR
CCAATGAAGAAGCCTTAAAA+AGG 0.292236 6_1:-1520063 None:intergenic
AAGCAATGGTGGTGGTGTTT+AGG 0.302399 6_1:-1519369 None:intergenic
ATTGGGAATGTAAGATGTTT+GGG 0.318124 6_1:+1520081 Msa0868060:three_prime_UTR
AACACCACCACCATTGCTTC+TGG 0.327365 6_1:+1519373 Msa0868060:CDS
AGCAATGGTGGTGGTGTTTA+GGG 0.333049 6_1:-1519368 None:intergenic
ATGATTAAACCGGGGTGAAC+CGG 0.350078 6_1:-1519710 None:intergenic
AGATGTTTGGGACTTGGGTT+GGG 0.351631 6_1:+1520093 Msa0868060:three_prime_UTR
GCGGAGCAGAAGAAGGTTGC+TGG 0.381489 6_1:+1519617 Msa0868060:CDS
CAGAAGAAGGTTGCTGGGCC+AGG 0.385682 6_1:+1519623 Msa0868060:CDS
AAACGTTGCCAGAACCGAAC+AGG 0.401440 6_1:+1519873 Msa0868060:three_prime_UTR
ATGTAAGATGTTTGGGACTT+GGG 0.402279 6_1:+1520088 Msa0868060:three_prime_UTR
CTCAAGACGTATGATTAAAC+CGG 0.405928 6_1:-1519720 None:intergenic
GAGAAGAAATGAACCTAAAG+AGG 0.416992 6_1:-1519116 None:intergenic
GGGTGAACCGGAACGTGAAC+CGG 0.417630 6_1:-1519698 None:intergenic
AAGATGTTTGGGACTTGGGT+TGG 0.421077 6_1:+1520092 Msa0868060:three_prime_UTR
AATGTAAGATGTTTGGGACT+TGG 0.424168 6_1:+1520087 Msa0868060:three_prime_UTR
GAGGTTTGAATTGTTGGACT+TGG 0.433808 6_1:+1519995 Msa0868060:three_prime_UTR
TGAGAGTGAGAAAGAGAGAA+TGG 0.434630 6_1:-1519256 None:intergenic
ATGTCAACCGGTTCACGTTC+CGG 0.448654 6_1:+1519691 Msa0868060:CDS
AACAGTGAAGCATCAAGAAA+TGG 0.452869 6_1:+1519553 Msa0868060:CDS
GTTTGAATTGTTGGACTTGG+TGG 0.458192 6_1:+1519998 Msa0868060:three_prime_UTR
AAGCATCAAGAAATGGAAAA+CGG 0.474883 6_1:+1519560 Msa0868060:CDS
CTCTTCTTCCACGGTGATGT+CGG 0.479412 6_1:-1519587 None:intergenic
AATGACAAAATACTATGTTA+AGG 0.481772 6_1:+1520242 Msa0868060:three_prime_UTR
AGCATCAAGAAATGGAAAAC+GGG 0.486636 6_1:+1519561 Msa0868060:CDS
CGTGTAGATCCAAGTGTGAT+TGG 0.513041 6_1:+1519660 Msa0868060:CDS
GAAGCATGGCGTTGCAAGTG+TGG 0.519320 6_1:+1519752 Msa0868060:CDS
AGAGGTGGCGGAGCAGAAGA+AGG 0.519433 6_1:+1519610 Msa0868060:CDS
TTGGTGTAACCCATGTCAAC+CGG 0.519681 6_1:+1519679 Msa0868060:CDS
GGAACGTGAACCGGTTGACA+TGG 0.526123 6_1:-1519689 None:intergenic
AGTGAGAAAGAGAGAATGGA+AGG 0.528050 6_1:-1519252 None:intergenic
TACTTATCAATTCATCATCA+AGG 0.529658 6_1:-1519791 None:intergenic
GCCACCTCTCTCTTCTTCCA+CGG 0.532769 6_1:-1519596 None:intergenic
CGGAGCAGAAGAAGGTTGCT+GGG 0.544379 6_1:+1519618 Msa0868060:CDS
GTATTATTAAATGTTACTGC+AGG 0.545034 6_1:-1519223 None:intergenic
GTGGAAGAAGAGAGAGGTGG+CGG 0.545993 6_1:+1519598 Msa0868060:CDS
TTGTGGTGCGAGAAGAGTTA+TGG 0.548135 6_1:-1519439 None:intergenic
AGAGTACAGAGAAGATGAAG+AGG 0.551498 6_1:-1519287 None:intergenic
AACTTGAACTTACTAGGTTG+TGG 0.559169 6_1:-1519456 None:intergenic
AGAAGAAGGTTGCTGGGCCA+GGG 0.561033 6_1:+1519624 Msa0868060:CDS
AAGAGAGAATGGAAGGTGTG+TGG 0.576934 6_1:-1519245 None:intergenic
GGTTCTCTGTTGTGTAGAGA+GGG 0.587339 6_1:-1520305 None:intergenic
TGGTTCTCTGTTGTGTAGAG+AGG 0.588574 6_1:-1520306 None:intergenic
GAAATGAACCTAAAGAGGGA+AGG 0.600783 6_1:-1519111 None:intergenic
TCAAGACGTATGATTAAACC+GGG 0.608793 6_1:-1519719 None:intergenic
TGGGTTACACCAATCACACT+TGG 0.609884 6_1:-1519669 None:intergenic
AAGCATGGCGTTGCAAGTGT+GGG 0.610762 6_1:+1519753 Msa0868060:CDS
AGAAGAAATGAACCTAAAGA+GGG 0.612120 6_1:-1519115 None:intergenic
CACGACGGAGGTGTTGACCC+TGG 0.613267 6_1:-1519641 None:intergenic
GAACGTGAACCGGTTGACAT+GGG 0.614728 6_1:-1519688 None:intergenic
GTACGCACATATTGATGAGG+TGG 0.617360 6_1:-1519338 None:intergenic
ATAGAAGAAGAATAACAAGA+TGG 0.617872 6_1:-1520326 None:intergenic
TTGAGTATTATCCAGAAGCA+TGG 0.618402 6_1:+1519738 Msa0868060:CDS
TACTACTATGTACAATAGAG+AGG 0.621909 6_1:-1520145 None:intergenic
AACGTTGCCAGAACCGAACA+GGG 0.633112 6_1:+1519874 Msa0868060:three_prime_UTR
TGGGTAATAACCAGAAGCAA+TGG 0.633930 6_1:-1519383 None:intergenic
GTTCACGTTCCGGTTCACCC+CGG 0.635691 6_1:+1519701 Msa0868060:CDS
ATAACCAGAAGCAATGGTGG+TGG 0.639918 6_1:-1519377 None:intergenic
GTAATAACCAGAAGCAATGG+TGG 0.650622 6_1:-1519380 None:intergenic
AATGGTGGTGGTGTTTAGGG+TGG 0.653961 6_1:-1519365 None:intergenic
ACTACTATGTACAATAGAGA+GGG 0.659336 6_1:-1520144 None:intergenic
GCAGTACGCACATATTGATG+AGG 0.669919 6_1:-1519341 None:intergenic
GTATTAATGATGTTGCTCTG+AGG 0.673923 6_1:+1519976 Msa0868060:three_prime_UTR
ACCGTGGAAGAAGAGAGAGG+TGG 0.701228 6_1:+1519595 Msa0868060:CDS
ATCACCGTGGAAGAAGAGAG+AGG 0.712922 6_1:+1519592 Msa0868060:CDS
CACTTGGATCTACACGACGG+AGG 0.750576 6_1:-1519653 None:intergenic
TCACACTTGGATCTACACGA+CGG 0.754283 6_1:-1519656 None:intergenic
ACGGGTCTCCGACATCACCG+TGG 0.754489 6_1:+1519579 Msa0868060:CDS
CAAGACGTATGATTAAACCG+GGG 0.816477 6_1:-1519718 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTACAATAAAAACATTAAA+AGG - chr6_1:1520220-1520239 None:intergenic 10.0%
!! TTGTAATTATATGAATTATT+TGG + chr6_1:1520279-1520298 Msa0868060:three_prime_UTR 10.0%
!! TTTACAATAAAAACATTAAA+AGG - chr6_1:1520220-1520239 None:intergenic 10.0%
!! TTGTAATTATATGAATTATT+TGG + chr6_1:1520279-1520298 Msa0868060:three_prime_UTR 10.0%
!!! TGAGTACTAATATTGATTTT+TGG + chr6_1:1520035-1520054 Msa0868060:three_prime_UTR 20.0%
!!! TTTTTGGTTTAACCTTTTTA+AGG + chr6_1:1520051-1520070 Msa0868060:three_prime_UTR 20.0%
!!! TCTATTTCCTTTTTTGTTTT+AGG + chr6_1:1520175-1520194 Msa0868060:three_prime_UTR 20.0%
!! ACATAAACCTAAAACAAAAA+AGG - chr6_1:1520185-1520204 None:intergenic 20.0%
!! ACAATAAAAACATTAAAAGG+AGG - chr6_1:1520217-1520236 None:intergenic 20.0%
!! AATGACAAAATACTATGTTA+AGG + chr6_1:1520242-1520261 Msa0868060:three_prime_UTR 20.0%
!!! TGAGTACTAATATTGATTTT+TGG + chr6_1:1520035-1520054 Msa0868060:three_prime_UTR 20.0%
!!! TTTTTGGTTTAACCTTTTTA+AGG + chr6_1:1520051-1520070 Msa0868060:three_prime_UTR 20.0%
!!! TCTATTTCCTTTTTTGTTTT+AGG + chr6_1:1520175-1520194 Msa0868060:three_prime_UTR 20.0%
!! ACATAAACCTAAAACAAAAA+AGG - chr6_1:1520185-1520204 None:intergenic 20.0%
!! ACAATAAAAACATTAAAAGG+AGG - chr6_1:1520217-1520236 None:intergenic 20.0%
!! AATGACAAAATACTATGTTA+AGG + chr6_1:1520242-1520261 Msa0868060:three_prime_UTR 20.0%
! GTATTATTAAATGTTACTGC+AGG - chr6_1:1519226-1519245 None:intergenic 25.0%
! AGAAAACAAGAGAAAAATGA+AGG - chr6_1:1519409-1519428 None:intergenic 25.0%
!! TGAAAAAAGTATGAAGAAAG+TGG - chr6_1:1519497-1519516 None:intergenic 25.0%
! TACTTATCAATTCATCATCA+AGG - chr6_1:1519794-1519813 None:intergenic 25.0%
!!! TGTGTTTTTTCTTTTGTACT+AGG - chr6_1:1519854-1519873 None:intergenic 25.0%
! AAAAGGGGAAAAATTTAAGA+TGG - chr6_1:1519917-1519936 None:intergenic 25.0%
!! ATCTTAAATTTTTCCCCTTT+TGG + chr6_1:1519916-1519935 Msa0868060:three_prime_UTR 25.0%
!! TCTTAAATTTTTCCCCTTTT+GGG + chr6_1:1519917-1519936 Msa0868060:three_prime_UTR 25.0%
! TAATACAAAAAAGCCCAAAA+GGG - chr6_1:1519933-1519952 None:intergenic 25.0%
! ATAATACAAAAAAGCCCAAA+AGG - chr6_1:1519934-1519953 None:intergenic 25.0%
! GTATTATTAAATGTTACTGC+AGG - chr6_1:1519226-1519245 None:intergenic 25.0%
! AGAAAACAAGAGAAAAATGA+AGG - chr6_1:1519409-1519428 None:intergenic 25.0%
!! TGAAAAAAGTATGAAGAAAG+TGG - chr6_1:1519497-1519516 None:intergenic 25.0%
! TACTTATCAATTCATCATCA+AGG - chr6_1:1519794-1519813 None:intergenic 25.0%
!!! TGTGTTTTTTCTTTTGTACT+AGG - chr6_1:1519854-1519873 None:intergenic 25.0%
! AAAAGGGGAAAAATTTAAGA+TGG - chr6_1:1519917-1519936 None:intergenic 25.0%
!! ATCTTAAATTTTTCCCCTTT+TGG + chr6_1:1519916-1519935 Msa0868060:three_prime_UTR 25.0%
!! TCTTAAATTTTTCCCCTTTT+GGG + chr6_1:1519917-1519936 Msa0868060:three_prime_UTR 25.0%
! TAATACAAAAAAGCCCAAAA+GGG - chr6_1:1519933-1519952 None:intergenic 25.0%
! ATAATACAAAAAAGCCCAAA+AGG - chr6_1:1519934-1519953 None:intergenic 25.0%
AGAAGAAATGAACCTAAAGA+GGG - chr6_1:1519118-1519137 None:intergenic 30.0%
!! TCATTTCTTCTCTTTTGACT+TGG + chr6_1:1519127-1519146 Msa0868060:five_prime_UTR 30.0%
AACAAGAGAAAAATGAAGGT+GGG - chr6_1:1519405-1519424 None:intergenic 30.0%
AAACAAGAGAAAAATGAAGG+TGG - chr6_1:1519406-1519425 None:intergenic 30.0%
GAAATGAACTTGAACTTACT+AGG - chr6_1:1519465-1519484 None:intergenic 30.0%
! CAACAACTCTTTTCATTTTC+AGG + chr6_1:1519521-1519540 Msa0868060:intron 30.0%
!!! TTTTCATTTTCAGGTAGTAG+TGG + chr6_1:1519530-1519549 Msa0868060:intron 30.0%
AAGCATCAAGAAATGGAAAA+CGG + chr6_1:1519560-1519579 Msa0868060:CDS 30.0%
AATACAAAAAAGCCCAAAAG+GGG - chr6_1:1519932-1519951 None:intergenic 30.0%
!!! CTTTTTAAGGCTTCTTCATT+GGG + chr6_1:1520064-1520083 Msa0868060:three_prime_UTR 30.0%
ATTGGGAATGTAAGATGTTT+GGG + chr6_1:1520081-1520100 Msa0868060:three_prime_UTR 30.0%
ACTACTATGTACAATAGAGA+GGG - chr6_1:1520147-1520166 None:intergenic 30.0%
TACTACTATGTACAATAGAG+AGG - chr6_1:1520148-1520167 None:intergenic 30.0%
! ATATCTTGTTTGAGTATCTG+AGG - chr6_1:1519081-1519100 None:intergenic 30.0%
AGAAGAAATGAACCTAAAGA+GGG - chr6_1:1519118-1519137 None:intergenic 30.0%
!! TCATTTCTTCTCTTTTGACT+TGG + chr6_1:1519127-1519146 Msa0868060:five_prime_UTR 30.0%
AACAAGAGAAAAATGAAGGT+GGG - chr6_1:1519405-1519424 None:intergenic 30.0%
AAACAAGAGAAAAATGAAGG+TGG - chr6_1:1519406-1519425 None:intergenic 30.0%
GAAATGAACTTGAACTTACT+AGG - chr6_1:1519465-1519484 None:intergenic 30.0%
! CAACAACTCTTTTCATTTTC+AGG + chr6_1:1519521-1519540 Msa0868060:intron 30.0%
!!! TTTTCATTTTCAGGTAGTAG+TGG + chr6_1:1519530-1519549 Msa0868060:intron 30.0%
AAGCATCAAGAAATGGAAAA+CGG + chr6_1:1519560-1519579 Msa0868060:CDS 30.0%
AATACAAAAAAGCCCAAAAG+GGG - chr6_1:1519932-1519951 None:intergenic 30.0%
!!! CTTTTTAAGGCTTCTTCATT+GGG + chr6_1:1520064-1520083 Msa0868060:three_prime_UTR 30.0%
ATTGGGAATGTAAGATGTTT+GGG + chr6_1:1520081-1520100 Msa0868060:three_prime_UTR 30.0%
ACTACTATGTACAATAGAGA+GGG - chr6_1:1520147-1520166 None:intergenic 30.0%
TACTACTATGTACAATAGAG+AGG - chr6_1:1520148-1520167 None:intergenic 30.0%
GAGAAGAAATGAACCTAAAG+AGG - chr6_1:1519119-1519138 None:intergenic 35.0%
AACTTGAACTTACTAGGTTG+TGG - chr6_1:1519459-1519478 None:intergenic 35.0%
! AACAGTGAAGCATCAAGAAA+TGG + chr6_1:1519553-1519572 Msa0868060:CDS 35.0%
AGCATCAAGAAATGGAAAAC+GGG + chr6_1:1519561-1519580 Msa0868060:CDS 35.0%
TCAAGACGTATGATTAAACC+GGG - chr6_1:1519722-1519741 None:intergenic 35.0%
CTCAAGACGTATGATTAAAC+CGG - chr6_1:1519723-1519742 None:intergenic 35.0%
TTGAGTATTATCCAGAAGCA+TGG + chr6_1:1519738-1519757 Msa0868060:CDS 35.0%
GTATTAATGATGTTGCTCTG+AGG + chr6_1:1519976-1519995 Msa0868060:three_prime_UTR 35.0%
CCAATGAAGAAGCCTTAAAA+AGG - chr6_1:1520066-1520085 None:intergenic 35.0%
!!! CCTTTTTAAGGCTTCTTCAT+TGG + chr6_1:1520063-1520082 Msa0868060:three_prime_UTR 35.0%
CATTGGGAATGTAAGATGTT+TGG + chr6_1:1520080-1520099 Msa0868060:three_prime_UTR 35.0%
! AATGTAAGATGTTTGGGACT+TGG + chr6_1:1520087-1520106 Msa0868060:three_prime_UTR 35.0%
! ATGTAAGATGTTTGGGACTT+GGG + chr6_1:1520088-1520107 Msa0868060:three_prime_UTR 35.0%
GAGAAGAAATGAACCTAAAG+AGG - chr6_1:1519119-1519138 None:intergenic 35.0%
AACTTGAACTTACTAGGTTG+TGG - chr6_1:1519459-1519478 None:intergenic 35.0%
! AACAGTGAAGCATCAAGAAA+TGG + chr6_1:1519553-1519572 Msa0868060:CDS 35.0%
AGCATCAAGAAATGGAAAAC+GGG + chr6_1:1519561-1519580 Msa0868060:CDS 35.0%
TCAAGACGTATGATTAAACC+GGG - chr6_1:1519722-1519741 None:intergenic 35.0%
CTCAAGACGTATGATTAAAC+CGG - chr6_1:1519723-1519742 None:intergenic 35.0%
TTGAGTATTATCCAGAAGCA+TGG + chr6_1:1519738-1519757 Msa0868060:CDS 35.0%
GTATTAATGATGTTGCTCTG+AGG + chr6_1:1519976-1519995 Msa0868060:three_prime_UTR 35.0%
CCAATGAAGAAGCCTTAAAA+AGG - chr6_1:1520066-1520085 None:intergenic 35.0%
!!! CCTTTTTAAGGCTTCTTCAT+TGG + chr6_1:1520063-1520082 Msa0868060:three_prime_UTR 35.0%
CATTGGGAATGTAAGATGTT+TGG + chr6_1:1520080-1520099 Msa0868060:three_prime_UTR 35.0%
! AATGTAAGATGTTTGGGACT+TGG + chr6_1:1520087-1520106 Msa0868060:three_prime_UTR 35.0%
! ATGTAAGATGTTTGGGACTT+GGG + chr6_1:1520088-1520107 Msa0868060:three_prime_UTR 35.0%
GAAATGAACCTAAAGAGGGA+AGG - chr6_1:1519114-1519133 None:intergenic 40.0%
! CTCACATTTTCACAACACAC+AGG + chr6_1:1519167-1519186 Msa0868060:five_prime_UTR 40.0%
AGTGAGAAAGAGAGAATGGA+AGG - chr6_1:1519255-1519274 None:intergenic 40.0%
TGAGAGTGAGAAAGAGAGAA+TGG - chr6_1:1519259-1519278 None:intergenic 40.0%
AGAGTACAGAGAAGATGAAG+AGG - chr6_1:1519290-1519309 None:intergenic 40.0%
GTAATAACCAGAAGCAATGG+TGG - chr6_1:1519383-1519402 None:intergenic 40.0%
TGGGTAATAACCAGAAGCAA+TGG - chr6_1:1519386-1519405 None:intergenic 40.0%
CAAGACGTATGATTAAACCG+GGG - chr6_1:1519721-1519740 None:intergenic 40.0%
! TCTGCAACATTTTCCCTGTT+CGG - chr6_1:1519890-1519909 None:intergenic 40.0%
!! TGCTCTGAGGTTTGAATTGT+TGG + chr6_1:1519989-1520008 Msa0868060:three_prime_UTR 40.0%
GAGGTTTGAATTGTTGGACT+TGG + chr6_1:1519995-1520014 Msa0868060:three_prime_UTR 40.0%
GTTTGAATTGTTGGACTTGG+TGG + chr6_1:1519998-1520017 Msa0868060:three_prime_UTR 40.0%
GAAATGAACCTAAAGAGGGA+AGG - chr6_1:1519114-1519133 None:intergenic 40.0%
! CTCACATTTTCACAACACAC+AGG + chr6_1:1519167-1519186 Msa0868060:five_prime_UTR 40.0%
AGTGAGAAAGAGAGAATGGA+AGG - chr6_1:1519255-1519274 None:intergenic 40.0%
TGAGAGTGAGAAAGAGAGAA+TGG - chr6_1:1519259-1519278 None:intergenic 40.0%
AGAGTACAGAGAAGATGAAG+AGG - chr6_1:1519290-1519309 None:intergenic 40.0%
GTAATAACCAGAAGCAATGG+TGG - chr6_1:1519383-1519402 None:intergenic 40.0%
TGGGTAATAACCAGAAGCAA+TGG - chr6_1:1519386-1519405 None:intergenic 40.0%
CAAGACGTATGATTAAACCG+GGG - chr6_1:1519721-1519740 None:intergenic 40.0%
! TCTGCAACATTTTCCCTGTT+CGG - chr6_1:1519890-1519909 None:intergenic 40.0%
!! TGCTCTGAGGTTTGAATTGT+TGG + chr6_1:1519989-1520008 Msa0868060:three_prime_UTR 40.0%
GAGGTTTGAATTGTTGGACT+TGG + chr6_1:1519995-1520014 Msa0868060:three_prime_UTR 40.0%
GTTTGAATTGTTGGACTTGG+TGG + chr6_1:1519998-1520017 Msa0868060:three_prime_UTR 40.0%
! AAGAGAGAATGGAAGGTGTG+TGG - chr6_1:1519248-1519267 None:intergenic 45.0%
GTACGCACATATTGATGAGG+TGG - chr6_1:1519341-1519360 None:intergenic 45.0%
GCAGTACGCACATATTGATG+AGG - chr6_1:1519344-1519363 None:intergenic 45.0%
!! AGCAATGGTGGTGGTGTTTA+GGG - chr6_1:1519371-1519390 None:intergenic 45.0%
!! AAGCAATGGTGGTGGTGTTT+AGG - chr6_1:1519372-1519391 None:intergenic 45.0%
ATAACCAGAAGCAATGGTGG+TGG - chr6_1:1519380-1519399 None:intergenic 45.0%
TTGTGGTGCGAGAAGAGTTA+TGG - chr6_1:1519442-1519461 None:intergenic 45.0%
TCACACTTGGATCTACACGA+CGG - chr6_1:1519659-1519678 None:intergenic 45.0%
CGTGTAGATCCAAGTGTGAT+TGG + chr6_1:1519660-1519679 Msa0868060:CDS 45.0%
TGGGTTACACCAATCACACT+TGG - chr6_1:1519672-1519691 None:intergenic 45.0%
TTGGTGTAACCCATGTCAAC+CGG + chr6_1:1519679-1519698 Msa0868060:CDS 45.0%
ATGATTAAACCGGGGTGAAC+CGG - chr6_1:1519713-1519732 None:intergenic 45.0%
! ACATTTTCCCTGTTCGGTTC+TGG - chr6_1:1519884-1519903 None:intergenic 45.0%
! AAGATGTTTGGGACTTGGGT+TGG + chr6_1:1520092-1520111 Msa0868060:three_prime_UTR 45.0%
! AGATGTTTGGGACTTGGGTT+GGG + chr6_1:1520093-1520112 Msa0868060:three_prime_UTR 45.0%
GGTTCTCTGTTGTGTAGAGA+GGG - chr6_1:1520308-1520327 None:intergenic 45.0%
TGGTTCTCTGTTGTGTAGAG+AGG - chr6_1:1520309-1520328 None:intergenic 45.0%
! AAGAGAGAATGGAAGGTGTG+TGG - chr6_1:1519248-1519267 None:intergenic 45.0%
GTACGCACATATTGATGAGG+TGG - chr6_1:1519341-1519360 None:intergenic 45.0%
GCAGTACGCACATATTGATG+AGG - chr6_1:1519344-1519363 None:intergenic 45.0%
!! AGCAATGGTGGTGGTGTTTA+GGG - chr6_1:1519371-1519390 None:intergenic 45.0%
!! AAGCAATGGTGGTGGTGTTT+AGG - chr6_1:1519372-1519391 None:intergenic 45.0%
ATAACCAGAAGCAATGGTGG+TGG - chr6_1:1519380-1519399 None:intergenic 45.0%
TTGTGGTGCGAGAAGAGTTA+TGG - chr6_1:1519442-1519461 None:intergenic 45.0%
TCACACTTGGATCTACACGA+CGG - chr6_1:1519659-1519678 None:intergenic 45.0%
CGTGTAGATCCAAGTGTGAT+TGG + chr6_1:1519660-1519679 Msa0868060:CDS 45.0%
TGGGTTACACCAATCACACT+TGG - chr6_1:1519672-1519691 None:intergenic 45.0%
TTGGTGTAACCCATGTCAAC+CGG + chr6_1:1519679-1519698 Msa0868060:CDS 45.0%
ATGATTAAACCGGGGTGAAC+CGG - chr6_1:1519713-1519732 None:intergenic 45.0%
! ACATTTTCCCTGTTCGGTTC+TGG - chr6_1:1519884-1519903 None:intergenic 45.0%
! AAGATGTTTGGGACTTGGGT+TGG + chr6_1:1520092-1520111 Msa0868060:three_prime_UTR 45.0%
! AGATGTTTGGGACTTGGGTT+GGG + chr6_1:1520093-1520112 Msa0868060:three_prime_UTR 45.0%
GGTTCTCTGTTGTGTAGAGA+GGG - chr6_1:1520308-1520327 None:intergenic 45.0%
TGGTTCTCTGTTGTGTAGAG+AGG - chr6_1:1520309-1520328 None:intergenic 45.0%
CTCTCTCTCCTTCCCTCTTT+AGG + chr6_1:1519103-1519122 Msa0868060:five_prime_UTR 50.0%
!! AATGGTGGTGGTGTTTAGGG+TGG - chr6_1:1519368-1519387 None:intergenic 50.0%
AACACCACCACCATTGCTTC+TGG + chr6_1:1519373-1519392 Msa0868060:CDS 50.0%
CTCTTCTTCCACGGTGATGT+CGG - chr6_1:1519590-1519609 None:intergenic 50.0%
ATCACCGTGGAAGAAGAGAG+AGG + chr6_1:1519592-1519611 Msa0868060:CDS 50.0%
GAACGTGAACCGGTTGACAT+GGG - chr6_1:1519691-1519710 None:intergenic 50.0%
! ATGTCAACCGGTTCACGTTC+CGG + chr6_1:1519691-1519710 Msa0868060:CDS 50.0%
AAGCATGGCGTTGCAAGTGT+GGG + chr6_1:1519753-1519772 Msa0868060:CDS 50.0%
AAACGTTGCCAGAACCGAAC+AGG + chr6_1:1519873-1519892 Msa0868060:three_prime_UTR 50.0%
AACGTTGCCAGAACCGAACA+GGG + chr6_1:1519874-1519893 Msa0868060:three_prime_UTR 50.0%
!! TTTGGGACTTGGGTTGGGTT+TGG + chr6_1:1520098-1520117 Msa0868060:three_prime_UTR 50.0%
CTCTCTCTCCTTCCCTCTTT+AGG + chr6_1:1519103-1519122 Msa0868060:five_prime_UTR 50.0%
!! AATGGTGGTGGTGTTTAGGG+TGG - chr6_1:1519368-1519387 None:intergenic 50.0%
AACACCACCACCATTGCTTC+TGG + chr6_1:1519373-1519392 Msa0868060:CDS 50.0%
CTCTTCTTCCACGGTGATGT+CGG - chr6_1:1519590-1519609 None:intergenic 50.0%
ATCACCGTGGAAGAAGAGAG+AGG + chr6_1:1519592-1519611 Msa0868060:CDS 50.0%
GAACGTGAACCGGTTGACAT+GGG - chr6_1:1519691-1519710 None:intergenic 50.0%
! ATGTCAACCGGTTCACGTTC+CGG + chr6_1:1519691-1519710 Msa0868060:CDS 50.0%
AAGCATGGCGTTGCAAGTGT+GGG + chr6_1:1519753-1519772 Msa0868060:CDS 50.0%
AAACGTTGCCAGAACCGAAC+AGG + chr6_1:1519873-1519892 Msa0868060:three_prime_UTR 50.0%
AACGTTGCCAGAACCGAACA+GGG + chr6_1:1519874-1519893 Msa0868060:three_prime_UTR 50.0%
!! TTTGGGACTTGGGTTGGGTT+TGG + chr6_1:1520098-1520117 Msa0868060:three_prime_UTR 50.0%
ACCGTGGAAGAAGAGAGAGG+TGG + chr6_1:1519595-1519614 Msa0868060:CDS 55.0%
GCCACCTCTCTCTTCTTCCA+CGG - chr6_1:1519599-1519618 None:intergenic 55.0%
GTGGAAGAAGAGAGAGGTGG+CGG + chr6_1:1519598-1519617 Msa0868060:CDS 55.0%
CGGAGCAGAAGAAGGTTGCT+GGG + chr6_1:1519618-1519637 Msa0868060:CDS 55.0%
AGAAGAAGGTTGCTGGGCCA+GGG + chr6_1:1519624-1519643 Msa0868060:CDS 55.0%
CACTTGGATCTACACGACGG+AGG - chr6_1:1519656-1519675 None:intergenic 55.0%
GGAACGTGAACCGGTTGACA+TGG - chr6_1:1519692-1519711 None:intergenic 55.0%
CACTTGCAACGCCATGCTTC+TGG - chr6_1:1519752-1519771 None:intergenic 55.0%
GAAGCATGGCGTTGCAAGTG+TGG + chr6_1:1519752-1519771 Msa0868060:CDS 55.0%
ACCGTGGAAGAAGAGAGAGG+TGG + chr6_1:1519595-1519614 Msa0868060:CDS 55.0%
GCCACCTCTCTCTTCTTCCA+CGG - chr6_1:1519599-1519618 None:intergenic 55.0%
GTGGAAGAAGAGAGAGGTGG+CGG + chr6_1:1519598-1519617 Msa0868060:CDS 55.0%
CGGAGCAGAAGAAGGTTGCT+GGG + chr6_1:1519618-1519637 Msa0868060:CDS 55.0%
AGAAGAAGGTTGCTGGGCCA+GGG + chr6_1:1519624-1519643 Msa0868060:CDS 55.0%
CACTTGGATCTACACGACGG+AGG - chr6_1:1519656-1519675 None:intergenic 55.0%
GGAACGTGAACCGGTTGACA+TGG - chr6_1:1519692-1519711 None:intergenic 55.0%
CACTTGCAACGCCATGCTTC+TGG - chr6_1:1519752-1519771 None:intergenic 55.0%
GAAGCATGGCGTTGCAAGTG+TGG + chr6_1:1519752-1519771 Msa0868060:CDS 55.0%
!! AGAGGTGGCGGAGCAGAAGA+AGG + chr6_1:1519610-1519629 Msa0868060:CDS 60.0%
GCGGAGCAGAAGAAGGTTGC+TGG + chr6_1:1519617-1519636 Msa0868060:CDS 60.0%
CAGAAGAAGGTTGCTGGGCC+AGG + chr6_1:1519623-1519642 Msa0868060:CDS 60.0%
GGGTGAACCGGAACGTGAAC+CGG - chr6_1:1519701-1519720 None:intergenic 60.0%
GTTCACGTTCCGGTTCACCC+CGG + chr6_1:1519701-1519720 Msa0868060:CDS 60.0%
!! AGAGGTGGCGGAGCAGAAGA+AGG + chr6_1:1519610-1519629 Msa0868060:CDS 60.0%
GCGGAGCAGAAGAAGGTTGC+TGG + chr6_1:1519617-1519636 Msa0868060:CDS 60.0%
CAGAAGAAGGTTGCTGGGCC+AGG + chr6_1:1519623-1519642 Msa0868060:CDS 60.0%
GGGTGAACCGGAACGTGAAC+CGG - chr6_1:1519701-1519720 None:intergenic 60.0%
GTTCACGTTCCGGTTCACCC+CGG + chr6_1:1519701-1519720 Msa0868060:CDS 60.0%
ACGGGTCTCCGACATCACCG+TGG + chr6_1:1519579-1519598 Msa0868060:CDS 65.0%
!! CACGACGGAGGTGTTGACCC+TGG - chr6_1:1519644-1519663 None:intergenic 65.0%
ACGGGTCTCCGACATCACCG+TGG + chr6_1:1519579-1519598 Msa0868060:CDS 65.0%
!! CACGACGGAGGTGTTGACCC+TGG - chr6_1:1519644-1519663 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 1519079 1520342 1519079 ID=Msa0868060;Name=Msa0868060
chr6_1 mRNA 1519079 1520342 1519079 ID=Msa0868060-mRNA-1;Parent=Msa0868060;Name=Msa0868060-mRNA-1;_AED=0.00;_eAED=0.00;_QI=272|1|1|1|1|1|2|546|122
chr6_1 exon 1519079 1519465 1519079 ID=Msa0868060-mRNA-1:exon:416;Parent=Msa0868060-mRNA-1
chr6_1 exon 1519543 1520342 1519543 ID=Msa0868060-mRNA-1:exon:417;Parent=Msa0868060-mRNA-1
chr6_1 five_prime_UTR 1519079 1519350 1519079 ID=Msa0868060-mRNA-1:five_prime_utr;Parent=Msa0868060-mRNA-1
chr6_1 CDS 1519351 1519465 1519351 ID=Msa0868060-mRNA-1:cds;Parent=Msa0868060-mRNA-1
chr6_1 CDS 1519543 1519796 1519543 ID=Msa0868060-mRNA-1:cds;Parent=Msa0868060-mRNA-1
chr6_1 three_prime_UTR 1519797 1520342 1519797 ID=Msa0868060-mRNA-1:three_prime_utr;Parent=Msa0868060-mRNA-1
Gene Sequence

>Msa0868060

ATGTGCGTACTGCGCCACCCTAAACACCACCACCATTGCTTCTGGTTATTACCCACCTTCATTTTTCTCTTGTTTTCTTCTTCTTCAGCCATAACTCTTCTCGCACCACAACCTAGTAGTAGTGGAACAGTGAAGCATCAAGAAATGGAAAACGGGTCTCCGACATCACCGTGGAAGAAGAGAGAGGTGGCGGAGCAGAAGAAGGTTGCTGGGCCAGGGTCAACACCTCCGTCGTGTAGATCCAAGTGTGATTGGTGTAACCCATGTCAACCGGTTCACGTTCCGGTTCACCCCGGTTTAATCATACGTCTTGAGTATTATCCAGAAGCATGGCGTTGCAAGTGTGGGAATAAGTATTTTTTGCCTTGA
Protein sequence

>Msa0868060

MCVLRHPKHHHHCFWLLPTFIFLLFSSSSAITLLAPQPSSSGTVKHQEMENGSPTSPWKKREVAEQKKVAGPGSTPPSCRSKCDWCNPCQPVHVPVHPGLIIRLEYYPEAWRCKCGNKYFLP*