AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0040880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0040880 MtrunA17_Chr1g0206151 98.095 210 4 0 1 210 1 210 2.30e-151 418
Msa0040880 MtrunA17_Chr5g0394061 50.000 216 85 9 6 206 1 208 4.91e-54 172
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0040880 AT4G21215.2 42.529 174 78 9 51 206 23 192 2.30e-25 98.6
Msa0040880 AT4G21215.1 41.379 174 78 10 51 206 23 190 6.96e-22 89.4

Find 80 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTGAGAAATTTCGTCTTTA+AGG 0.044988 1_1:+71142330 None:intergenic
TCAGAATCAGTTAGCATTTC+TGG 0.094687 1_1:-71138861 Msa0040880:three_prime_UTR
TTTCTTTGGTGTGTTATATT+AGG 0.194196 1_1:-71138779 Msa0040880:three_prime_UTR
TACCGCATAAGATGAGATTT+TGG 0.242665 1_1:-71138965 Msa0040880:three_prime_UTR
GTGGATCATTTAGTTTAATT+TGG 0.259197 1_1:-71138364 Msa0040880:three_prime_UTR
TTCTTCTTCTTATGAGGCTT+TGG 0.277408 1_1:-71141935 Msa0040880:CDS
CACTTTCATAGAGGAAATAA+AGG 0.294942 1_1:+71142106 None:intergenic
TATTACCGTGGTTCTTGAAT+TGG 0.331796 1_1:+71141768 None:intergenic
AGATAGCTTTGTGATTGATA+TGG 0.346001 1_1:-71141830 Msa0040880:CDS
TTTGGTTTCTTCTTCTTATG+AGG 0.360374 1_1:-71141941 Msa0040880:CDS
TGAACTGTAGCTTCTCTGTT+AGG 0.382808 1_1:-71139482 Msa0040880:intron
ATTGATGATGTCTTTGTTGA+TGG 0.392741 1_1:+71141792 None:intergenic
CTTAGTATCAGCAAGCATAA+TGG 0.414607 1_1:-71139030 Msa0040880:CDS
ATAACACGGAGAAGTAGTTT+TGG 0.422585 1_1:-71139332 Msa0040880:CDS
GAATCAGAACAGATGAAGAT+AGG 0.422813 1_1:-71141913 Msa0040880:CDS
TTTAATTTGGTAAATTTATG+TGG 0.442531 1_1:-71138351 Msa0040880:three_prime_UTR
GACATAGATAGTGGAGTTGG+AGG 0.447412 1_1:-71138757 Msa0040880:three_prime_UTR
GTCAAAAGTTGCGTATTGAA+TGG 0.448696 1_1:+71142179 None:intergenic
AGTTGCAGCGGAGCCTATCT+AGG 0.453550 1_1:-71140435 Msa0040880:CDS
GCTGGGATCCTTTCCTAGAT+AGG 0.460020 1_1:+71140422 None:intergenic
AAAGCAGAATATAATGATAC+AGG 0.467930 1_1:+71138684 None:intergenic
TAGGACATAGATAGTGGAGT+TGG 0.468092 1_1:-71138760 Msa0040880:three_prime_UTR
CCTTTCGGAGAACATGTAGT+AGG 0.468709 1_1:+71140343 None:intergenic
ATCCACACACCTAATTGATT+CGG 0.474186 1_1:+71138381 None:intergenic
TAAAGAAAAGGAGAGGAGAA+AGG 0.476975 1_1:-71138593 Msa0040880:three_prime_UTR
AAGTGAAGATAATAAAGAAA+AGG 0.477462 1_1:-71138605 Msa0040880:three_prime_UTR
GATGAAAATAATGATGATGA+TGG 0.487796 1_1:+71142446 None:intergenic
ATTAAGATTCTTACCAACTC+AGG 0.492775 1_1:+71142065 None:intergenic
TATTTCCTCTATGAAAGTGA+TGG 0.495406 1_1:-71142102 Msa0040880:exon
TCAAAAGTTGCGTATTGAAT+GGG 0.502636 1_1:+71142180 None:intergenic
GATAGGTTCACAGAGAATCT+TGG 0.506776 1_1:-71141896 Msa0040880:CDS
TTATATTAGGACATAGATAG+TGG 0.508507 1_1:-71138766 Msa0040880:three_prime_UTR
ACTTGCTCAAATTCCTGAGT+TGG 0.513985 1_1:-71142078 Msa0040880:intron
TAGAAGACAGAGGAAAGAAC+GGG 0.514815 1_1:+71142396 None:intergenic
ATTTGATGATGTACTTGTTG+TGG 0.518420 1_1:+71139407 None:intergenic
GAGCAAGTCAACGGTGGTAC+CGG 0.521472 1_1:-71139456 Msa0040880:CDS
AAAGAACGGGTAAAAGAAGT+TGG 0.522934 1_1:+71142409 None:intergenic
TTCCGAATCAATTAGGTGTG+TGG 0.524740 1_1:-71138383 Msa0040880:three_prime_UTR
ACTTTCATAGAGGAAATAAA+GGG 0.530134 1_1:+71142107 None:intergenic
TGATGATGATGGAATGGAAG+TGG 0.531509 1_1:+71142457 None:intergenic
CTTAAAGACGAAATTTCTCA+CGG 0.543342 1_1:-71142329 Msa0040880:five_prime_UTR
CAAGTCAACGGTGGTACCGG+TGG 0.544727 1_1:-71139453 Msa0040880:CDS
TGATTCTGACATAAAGTTGG+TGG 0.553291 1_1:+71138875 None:intergenic
AAGTTCCATCACTTTCATAG+AGG 0.561165 1_1:+71142097 None:intergenic
AAATAATGATGATGATGGAA+TGG 0.562125 1_1:+71142451 None:intergenic
AACTGATTCTGACATAAAGT+TGG 0.567698 1_1:+71138872 None:intergenic
AAGTCAACGGTGGTACCGGT+GGG 0.567710 1_1:-71139452 Msa0040880:CDS
AAGATAATAAAGAAAAGGAG+AGG 0.568001 1_1:-71138600 Msa0040880:three_prime_UTR
CTAGGAAAGGATCCCAGCGT+GGG 0.587709 1_1:-71140417 Msa0040880:CDS
GTAGGTAGCTCTCATAGTCA+TGG 0.598179 1_1:+71138551 None:intergenic
GATACAAGAGGCAAATACAT+TGG 0.598258 1_1:-71138837 Msa0040880:three_prime_UTR
TCATCTTATGCGGTATAAGT+CGG 0.599570 1_1:+71138973 None:intergenic
GTAGAAGACAGAGGAAAGAA+CGG 0.600708 1_1:+71142395 None:intergenic
CTTTCGGAGAACATGTAGTA+GGG 0.607457 1_1:+71140344 None:intergenic
TAAACGATTTATAGTTGCAG+CGG 0.611666 1_1:-71140447 Msa0040880:intron
TTAAAGACGAAATTTCTCAC+GGG 0.613296 1_1:-71142328 Msa0040880:five_prime_UTR
CAGCGGAGCCTATCTAGGAA+AGG 0.618328 1_1:-71140430 Msa0040880:CDS
GAACGGGTAAAAGAAGTTGG+TGG 0.619369 1_1:+71142412 None:intergenic
AGCATTTCTGGAGATACAAG+AGG 0.622349 1_1:-71138849 Msa0040880:three_prime_UTR
GGTGAGGTATATAAATCCTA+CGG 0.624143 1_1:+71138705 None:intergenic
TGGATTTGTTGAATTCTGTG+TGG 0.627718 1_1:+71139427 None:intergenic
CAATGTCACACTTCAAGACA+AGG 0.628034 1_1:-71140374 Msa0040880:CDS
GAATTCTGTGTGGACCCCAC+CGG 0.630870 1_1:+71139437 None:intergenic
AAGCATAATGGAGTTGAACA+TGG 0.631540 1_1:-71139018 Msa0040880:CDS
AGTCAAGAATATTAGTAACA+AGG 0.635140 1_1:+71139053 None:intergenic
TTTATGCACCGTGATAGAGG+GGG 0.635330 1_1:-71138810 Msa0040880:three_prime_UTR
GTTAGGATCGAGCAAGTCAA+CGG 0.638158 1_1:-71139465 Msa0040880:CDS
TAGGAAAGGATCCCAGCGTG+GGG 0.640099 1_1:-71140416 Msa0040880:CDS
TCTAGGAAAGGATCCCAGCG+TGG 0.641016 1_1:-71140418 Msa0040880:CDS
AGTCAACGGTGGTACCGGTG+GGG 0.643482 1_1:-71139451 Msa0040880:CDS
TGCCAAAATCTCATCTTATG+CGG 0.644341 1_1:+71138963 None:intergenic
CAGAAAACAAAAGTATAACA+CGG 0.658180 1_1:-71139346 Msa0040880:CDS
AGAATATAATGATACAGGTG+AGG 0.658564 1_1:+71138689 None:intergenic
CCTACTACATGTTCTCCGAA+AGG 0.663390 1_1:-71140343 Msa0040880:intron
TTATGCACCGTGATAGAGGG+GGG 0.663865 1_1:-71138809 Msa0040880:three_prime_UTR
GCAAAGTTTGTAGAAGACAG+AGG 0.666959 1_1:+71142386 None:intergenic
CAATGCCAATTCAAGAACCA+CGG 0.685404 1_1:-71141773 Msa0040880:intron
AGGAAAGGATCCCAGCGTGG+GGG 0.705657 1_1:-71140415 Msa0040880:CDS
ATGTGACAGAAAGTGTGAAG+AGG 0.709402 1_1:-71138941 Msa0040880:three_prime_UTR
AGGATCGAGCAAGTCAACGG+TGG 0.738297 1_1:-71139462 Msa0040880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTATATTTTAACATAATA+TGG - chr1_1:71139320-71139339 Msa0040880:CDS 10.0%
!!! AAATTAACAACAAATTTTTT+TGG + chr1_1:71139426-71139445 None:intergenic 10.0%
!!! AAAAAAATTTGTTGTTAATT+TGG - chr1_1:71139425-71139444 Msa0040880:CDS 10.0%
!! TAAATCTTTCTAATAATAAT+AGG + chr1_1:71139593-71139612 None:intergenic 10.0%
!! AAAATTTACTAAAAAAAAGT+TGG + chr1_1:71139758-71139777 None:intergenic 10.0%
!!! TTTAGTAAATTTTTTTTTTG+TGG - chr1_1:71139765-71139784 Msa0040880:intron 10.0%
!! CTTAAAAATCATGATTAATT+AGG + chr1_1:71139241-71139260 None:intergenic 15.0%
!!! ATATTTTAACATAATATGGA+TGG - chr1_1:71139324-71139343 Msa0040880:CDS 15.0%
!! TTATACTAATAAAAGAAAAG+TGG + chr1_1:71139980-71139999 None:intergenic 15.0%
!! AATTAATTAAACGGATAATA+AGG - chr1_1:71140005-71140024 Msa0040880:intron 15.0%
!!! AATTGTTTCTTTTCATTTAT+TGG - chr1_1:71140540-71140559 Msa0040880:intron 15.0%
!!! ATTGTTTCTTTTCATTTATT+GGG - chr1_1:71140541-71140560 Msa0040880:intron 15.0%
!! AAAACACAACAAAATATATT+TGG + chr1_1:71140662-71140681 None:intergenic 15.0%
!! TTTATATTATCTTATGAATC+TGG - chr1_1:71140679-71140698 Msa0040880:intron 15.0%
!! AATACTTTGTATATTGAATA+TGG - chr1_1:71141291-71141310 Msa0040880:intron 15.0%
!! ATACTTTGTATATTGAATAT+GGG - chr1_1:71141292-71141311 Msa0040880:intron 15.0%
!! CAAAAAATAAAATTGTATCA+AGG + chr1_1:71141710-71141729 None:intergenic 15.0%
!!! CTTGATACAATTTTATTTTT+TGG - chr1_1:71141708-71141727 Msa0040880:intron 15.0%
!! AATTTACAAAAATGGAAAAA+AGG + chr1_1:71142176-71142195 None:intergenic 15.0%
!! TCACTTTAAATTTACAAAAA+TGG + chr1_1:71142184-71142203 None:intergenic 15.0%
!!! TTTAATTTGGTAAATTTATG+TGG - chr1_1:71142452-71142471 Msa0040880:five_prime_UTR 15.0%
!!! TGAATTTTCAATTTTCATTC+AGG - chr1_1:71138586-71138605 Msa0040880:three_prime_UTR 20.0%
!! ATATATAAGAGAAAAAAGTG+TGG + chr1_1:71138676-71138695 None:intergenic 20.0%
!! GATTTATCAAAGAATATTGA+AGG + chr1_1:71138804-71138823 None:intergenic 20.0%
!! AAAAAATCACAGTTATAAAG+TGG + chr1_1:71139657-71139676 None:intergenic 20.0%
!!! AGTAAATTTTTTTTTTGTGG+TGG - chr1_1:71139768-71139787 Msa0040880:intron 20.0%
!! GATTATAAATAAAGGGTTAA+AGG + chr1_1:71140115-71140134 None:intergenic 20.0%
!!! ATATTTGTTGTTCTTTCTTT+TGG - chr1_1:71140255-71140274 Msa0040880:intron 20.0%
!!! CTAAACTATAGTCTTAATAT+TGG + chr1_1:71140949-71140968 None:intergenic 20.0%
!! CAATATTAAGACTATAGTTT+AGG - chr1_1:71140947-71140966 Msa0040880:intron 20.0%
!!! ATATGCGATTAATATGTTTT+TGG - chr1_1:71141188-71141207 Msa0040880:intron 20.0%
!!! ACAATTTTATTTTTTGGAAC+AGG - chr1_1:71141714-71141733 Msa0040880:intron 20.0%
!! GAAGAGGAAAAAATTATATA+AGG - chr1_1:71141878-71141897 Msa0040880:CDS 20.0%
!! AAGTGAAGATAATAAAGAAA+AGG - chr1_1:71142198-71142217 Msa0040880:five_prime_UTR 20.0%
!!! AAATAATGATGATGATGGAA+TGG + chr1_1:71138355-71138374 None:intergenic 25.0%
!! GATGAAAATAATGATGATGA+TGG + chr1_1:71138360-71138379 None:intergenic 25.0%
!!! CTTTTTCTTTTGGTTTTTGT+TGG - chr1_1:71138500-71138519 Msa0040880:three_prime_UTR 25.0%
! ACTTTCATAGAGGAAATAAA+GGG + chr1_1:71138699-71138718 None:intergenic 25.0%
! TTCCATTGAATTTGAATTCT+GGG - chr1_1:71138778-71138797 Msa0040880:three_prime_UTR 25.0%
!!! TGTGTTTTTGTGTTTTTGAT+AGG - chr1_1:71138838-71138857 Msa0040880:three_prime_UTR 25.0%
!!! TTTGTGTTTTTGATAGGATT+TGG - chr1_1:71138844-71138863 Msa0040880:three_prime_UTR 25.0%
! AAGAATAAAAGTTATTACCG+TGG + chr1_1:71139050-71139069 None:intergenic 25.0%
! CATAGTGTATACTAAAAAAG+TGG + chr1_1:71139734-71139753 None:intergenic 25.0%
! AGGACACTAAATATTGAAAA+AGG - chr1_1:71139868-71139887 Msa0040880:intron 25.0%
! TATTGAAAAAGGAAAAACTG+TGG - chr1_1:71139879-71139898 Msa0040880:intron 25.0%
! AAATAAAGGGTTAAAGGAAA+AGG + chr1_1:71140109-71140128 None:intergenic 25.0%
! ATATTAAGTGCAATAACCAA+AGG + chr1_1:71140239-71140258 None:intergenic 25.0%
! TAAACCTCTTTATCTTTGTT+GGG + chr1_1:71140996-71141015 None:intergenic 25.0%
! TTAAACCTCTTTATCTTTGT+TGG + chr1_1:71140997-71141016 None:intergenic 25.0%
! AATGTCCAAATTTCTGATAA+TGG + chr1_1:71141244-71141263 None:intergenic 25.0%
!!! TATACTTTTGTTTTCTGTGT+TGG + chr1_1:71141454-71141473 None:intergenic 25.0%
!! CAGAAAACAAAAGTATAACA+CGG - chr1_1:71141457-71141476 Msa0040880:intron 25.0%
!!! ACACAAGAAGAACTTTTTTA+GGG + chr1_1:71141521-71141540 None:intergenic 25.0%
!!! AACACAAGAAGAACTTTTTT+AGG + chr1_1:71141522-71141541 None:intergenic 25.0%
! ATTTAGATTAAATGCGAGTT+TGG - chr1_1:71141601-71141620 Msa0040880:intron 25.0%
! AGTCAAGAATATTAGTAACA+AGG + chr1_1:71141753-71141772 None:intergenic 25.0%
!! TTTCTTTGGTGTGTTATATT+AGG - chr1_1:71142024-71142043 Msa0040880:intron 25.0%
! TTATATTAGGACATAGATAG+TGG - chr1_1:71142037-71142056 Msa0040880:intron 25.0%
! AAAGCAGAATATAATGATAC+AGG + chr1_1:71142122-71142141 None:intergenic 25.0%
! AAAAAGGTTCAAATGCTATT+AGG + chr1_1:71142160-71142179 None:intergenic 25.0%
! AAGATAATAAAGAAAAGGAG+AGG - chr1_1:71142203-71142222 Msa0040880:intron 25.0%
!! GAAATTTTTCCGAATCAATT+AGG - chr1_1:71142413-71142432 Msa0040880:five_prime_UTR 25.0%
!! GTGGATCATTTAGTTTAATT+TGG - chr1_1:71142439-71142458 Msa0040880:five_prime_UTR 25.0%
CTTAAAGACGAAATTTCTCA+CGG - chr1_1:71138474-71138493 Msa0040880:three_prime_UTR 30.0%
TTAAAGACGAAATTTCTCAC+GGG - chr1_1:71138475-71138494 Msa0040880:three_prime_UTR 30.0%
TCAAAAGTTGCGTATTGAAT+GGG + chr1_1:71138626-71138645 None:intergenic 30.0%
CACTTTCATAGAGGAAATAA+AGG + chr1_1:71138700-71138719 None:intergenic 30.0%
TATTTCCTCTATGAAAGTGA+TGG - chr1_1:71138701-71138720 Msa0040880:three_prime_UTR 30.0%
ATTAAGATTCTTACCAACTC+AGG + chr1_1:71138741-71138760 None:intergenic 30.0%
GTTCCATTGAATTTGAATTC+TGG - chr1_1:71138777-71138796 Msa0040880:three_prime_UTR 30.0%
! TTTGGTTTCTTCTTCTTATG+AGG - chr1_1:71138862-71138881 Msa0040880:three_prime_UTR 30.0%
!! AGATAGCTTTGTGATTGATA+TGG - chr1_1:71138973-71138992 Msa0040880:three_prime_UTR 30.0%
!! ATTGATGATGTCTTTGTTGA+TGG + chr1_1:71139014-71139033 None:intergenic 30.0%
AAAGTGGACATAGTGTAATA+TGG + chr1_1:71139964-71139983 None:intergenic 30.0%
! CAACTTTTGTTAAACCTTTG+CGG - chr1_1:71140038-71140057 Msa0040880:intron 30.0%
GAGAAAAATATTGCTTGAGA+AGG + chr1_1:71140081-71140100 None:intergenic 30.0%
CACACACAAAGTTAGTTAAT+TGG + chr1_1:71140173-71140192 None:intergenic 30.0%
AACAACTTAAGAATGCAACA+TGG + chr1_1:71140212-71140231 None:intergenic 30.0%
! TCTTAAGTTGTTCTTTCCTT+TGG - chr1_1:71140220-71140239 Msa0040880:intron 30.0%
TAAACGATTTATAGTTGCAG+CGG - chr1_1:71140356-71140375 Msa0040880:CDS 30.0%
TTAGAAGAAAACAATGAACG+AGG + chr1_1:71140514-71140533 None:intergenic 30.0%
! TCTTTTCATTTATTGGGACT+TGG - chr1_1:71140547-71140566 Msa0040880:intron 30.0%
CACTAGTAACTAAATTCAGA+TGG - chr1_1:71140829-71140848 Msa0040880:intron 30.0%
ATATCAAACATGATTACGCA+TGG - chr1_1:71141024-71141043 Msa0040880:intron 30.0%
! TTAGTGAAATTTGTTTGTCC+TGG - chr1_1:71141049-71141068 Msa0040880:intron 30.0%
ATGAGTTAAGGTGAAAAATC+AGG - chr1_1:71141088-71141107 Msa0040880:intron 30.0%
! GTTTGACAATTTTACGTAGT+AGG + chr1_1:71141117-71141136 None:intergenic 30.0%
!! TTTTTGGTACACCATACTTT+GGG - chr1_1:71141204-71141223 Msa0040880:intron 30.0%
ACTGTCCATTATCAGAAATT+TGG - chr1_1:71141236-71141255 Msa0040880:intron 30.0%
!! ATTTGATGATGTACTTGTTG+TGG + chr1_1:71141399-71141418 None:intergenic 30.0%
AAGATTTGAGTCTTACAATG+TGG + chr1_1:71141547-71141566 None:intergenic 30.0%
! AACTGATTCTGACATAAAGT+TGG + chr1_1:71141934-71141953 None:intergenic 30.0%
!!! TTTTTACTTTTTCTCTCCGT+AGG - chr1_1:71142082-71142101 Msa0040880:CDS 30.0%
AGAATATAATGATACAGGTG+AGG + chr1_1:71142117-71142136 None:intergenic 30.0%
!!! ACTTTTGTTTTGTAGCTTGT+AGG + chr1_1:71142273-71142292 None:intergenic 30.0%
!! AACCCACTAGTTTATTTTTC+AGG - chr1_1:71142367-71142386 Msa0040880:five_prime_UTR 30.0%
!! ACCCACTAGTTTATTTTTCA+GGG - chr1_1:71142368-71142387 Msa0040880:five_prime_UTR 30.0%
TCCCTGAAAAATAAACTAGT+GGG + chr1_1:71142372-71142391 None:intergenic 30.0%
AAAGAACGGGTAAAAGAAGT+TGG + chr1_1:71138397-71138416 None:intergenic 35.0%
!!! TCGTCTTTAAGGAGTGTTTT+TGG + chr1_1:71138465-71138484 None:intergenic 35.0%
CGTGAGAAATTTCGTCTTTA+AGG + chr1_1:71138476-71138495 None:intergenic 35.0%
GTCAAAAGTTGCGTATTGAA+TGG + chr1_1:71138627-71138646 None:intergenic 35.0%
AAGTTCCATCACTTTCATAG+AGG + chr1_1:71138709-71138728 None:intergenic 35.0%
TTCTTCTTCTTATGAGGCTT+TGG - chr1_1:71138868-71138887 Msa0040880:three_prime_UTR 35.0%
GAATCAGAACAGATGAAGAT+AGG - chr1_1:71138890-71138909 Msa0040880:three_prime_UTR 35.0%
TATTACCGTGGTTCTTGAAT+TGG + chr1_1:71139038-71139057 None:intergenic 35.0%
TTTGCATTTGTCATCTGAGT+TGG - chr1_1:71139933-71139952 Msa0040880:intron 35.0%
TTGCATTTGTCATCTGAGTT+GGG - chr1_1:71139934-71139953 Msa0040880:intron 35.0%
AGAAGGACATTCGATTCTAA+TGG + chr1_1:71140064-71140083 None:intergenic 35.0%
ACTTAAGAATGCAACATGGT+GGG + chr1_1:71140208-71140227 None:intergenic 35.0%
AACTTAAGAATGCAACATGG+TGG + chr1_1:71140209-71140228 None:intergenic 35.0%
AAGAAAACAATGAACGAGGT+CGG + chr1_1:71140510-71140529 None:intergenic 35.0%
!!! CTTGTTTTTGATGTGACACA+TGG - chr1_1:71140583-71140602 Msa0040880:intron 35.0%
CATGAAAACAAATCCTGAGT+TGG + chr1_1:71140718-71140737 None:intergenic 35.0%
CATATACTGTTTGACCGTTA+TGG - chr1_1:71140794-71140813 Msa0040880:intron 35.0%
ATATGAGCCATATGAATCAG+TGG + chr1_1:71140904-71140923 None:intergenic 35.0%
TCTTTATCTTTGTTGGGTCT+GGG + chr1_1:71140990-71141009 None:intergenic 35.0%
!!! GTTTTTGGTACACCATACTT+TGG - chr1_1:71141203-71141222 Msa0040880:intron 35.0%
! TTTTGGTACACCATACTTTG+GGG - chr1_1:71141205-71141224 Msa0040880:intron 35.0%
!! TGGATTTGTTGAATTCTGTG+TGG + chr1_1:71141379-71141398 None:intergenic 35.0%
! ATAACACGGAGAAGTAGTTT+TGG - chr1_1:71141471-71141490 Msa0040880:intron 35.0%
TTTGGCAAAGTACAAACTCA+CGG - chr1_1:71141619-71141638 Msa0040880:intron 35.0%
AAGGACTAACTAGTACAAAC+CGG + chr1_1:71141691-71141710 None:intergenic 35.0%
CTTAGTATCAGCAAGCATAA+TGG - chr1_1:71141773-71141792 Msa0040880:intron 35.0%
! AAGCATAATGGAGTTGAACA+TGG - chr1_1:71141785-71141804 Msa0040880:CDS 35.0%
TCATCTTATGCGGTATAAGT+CGG + chr1_1:71141833-71141852 None:intergenic 35.0%
! TACCGCATAAGATGAGATTT+TGG - chr1_1:71141838-71141857 Msa0040880:CDS 35.0%
TGCCAAAATCTCATCTTATG+CGG + chr1_1:71141843-71141862 None:intergenic 35.0%
TGATTCTGACATAAAGTTGG+TGG + chr1_1:71141931-71141950 None:intergenic 35.0%
TCAGAATCAGTTAGCATTTC+TGG - chr1_1:71141942-71141961 Msa0040880:CDS 35.0%
GATACAAGAGGCAAATACAT+TGG - chr1_1:71141966-71141985 Msa0040880:intron 35.0%
! ATTTTATGCACCGTGATAGA+GGG - chr1_1:71141991-71142010 Msa0040880:intron 35.0%
GGTGAGGTATATAAATCCTA+CGG + chr1_1:71142101-71142120 None:intergenic 35.0%
TAAAGAAAAGGAGAGGAGAA+AGG - chr1_1:71142210-71142229 Msa0040880:intron 35.0%
! TGCACGCTGAATGAAATTTT+TGG - chr1_1:71142325-71142344 Msa0040880:five_prime_UTR 35.0%
GTCCCTGAAAAATAAACTAG+TGG + chr1_1:71142373-71142392 None:intergenic 35.0%
ATCCACACACCTAATTGATT+CGG + chr1_1:71142425-71142444 None:intergenic 35.0%
!! TGATGATGATGGAATGGAAG+TGG + chr1_1:71138349-71138368 None:intergenic 40.0%
TAGAAGACAGAGGAAAGAAC+GGG + chr1_1:71138410-71138429 None:intergenic 40.0%
GTAGAAGACAGAGGAAAGAA+CGG + chr1_1:71138411-71138430 None:intergenic 40.0%
GCAAAGTTTGTAGAAGACAG+AGG + chr1_1:71138420-71138439 None:intergenic 40.0%
!!! CTCACGGGTACTTTTTCTTT+TGG - chr1_1:71138490-71138509 Msa0040880:three_prime_UTR 40.0%
ACTTGCTCAAATTCCTGAGT+TGG - chr1_1:71138725-71138744 Msa0040880:three_prime_UTR 40.0%
GGCCCAGAATTCAAATTCAA+TGG + chr1_1:71138783-71138802 None:intergenic 40.0%
GATAGGTTCACAGAGAATCT+TGG - chr1_1:71138907-71138926 Msa0040880:three_prime_UTR 40.0%
CAATGCCAATTCAAGAACCA+CGG - chr1_1:71139030-71139049 Msa0040880:CDS 40.0%
!! ACAGACTCTGTTTTGTTCGT+GGG + chr1_1:71139138-71139157 None:intergenic 40.0%
CATGAAATATGCAACGTCTG+GGG + chr1_1:71139806-71139825 None:intergenic 40.0%
GCATGAAATATGCAACGTCT+GGG + chr1_1:71139807-71139826 None:intergenic 40.0%
TGCATGAAATATGCAACGTC+TGG + chr1_1:71139808-71139827 None:intergenic 40.0%
!! GATCAAGCGTTGAAGATTAG+AGG - chr1_1:71140144-71140163 Msa0040880:intron 40.0%
CAATGTCACACTTCAAGACA+AGG - chr1_1:71140429-71140448 Msa0040880:CDS 40.0%
CTTTCGGAGAACATGTAGTA+GGG + chr1_1:71140462-71140481 None:intergenic 40.0%
! GTAACACTTTGGAAGGGAAA+TGG + chr1_1:71140488-71140507 None:intergenic 40.0%
! TCCTGAGTTGGCAAATCAAT+CGG + chr1_1:71140706-71140725 None:intergenic 40.0%
TGTTCACCAATGTGCCATAA+CGG + chr1_1:71140811-71140830 None:intergenic 40.0%
!! TTGGTGTCCACTGATTCATA+TGG - chr1_1:71140894-71140913 Msa0040880:intron 40.0%
CTCTTTATCTTTGTTGGGTC+TGG + chr1_1:71140991-71141010 None:intergenic 40.0%
CAGACCCAACAAAGATAAAG+AGG - chr1_1:71140989-71141008 Msa0040880:intron 40.0%
TAACTCATCTTCAACCACCA+GGG + chr1_1:71141076-71141095 None:intergenic 40.0%
TTAACTCATCTTCAACCACC+AGG + chr1_1:71141077-71141096 None:intergenic 40.0%
TGGTGGTTGAAGATGAGTTA+AGG - chr1_1:71141076-71141095 Msa0040880:intron 40.0%
!! CTGATAATGGACAGTACATG+TGG + chr1_1:71141231-71141250 None:intergenic 40.0%
TGAACTGTAGCTTCTCTGTT+AGG - chr1_1:71141321-71141340 Msa0040880:intron 40.0%
!! GTTTTGGACGATCTTCTTCA+TGG - chr1_1:71141487-71141506 Msa0040880:intron 40.0%
!!! TTTTGGAACAGGTCAAGTGT+AGG - chr1_1:71141725-71141744 Msa0040880:intron 40.0%
! ATGTGACAGAAAGTGTGAAG+AGG - chr1_1:71141862-71141881 Msa0040880:CDS 40.0%
! AGCATTTCTGGAGATACAAG+AGG - chr1_1:71141954-71141973 Msa0040880:intron 40.0%
! GATTTTATGCACCGTGATAG+AGG - chr1_1:71141990-71142009 Msa0040880:intron 40.0%
! TTTTATGCACCGTGATAGAG+GGG - chr1_1:71141992-71142011 Msa0040880:intron 40.0%
!! TAGGACATAGATAGTGGAGT+TGG - chr1_1:71142043-71142062 Msa0040880:intron 40.0%
TTCCGAATCAATTAGGTGTG+TGG - chr1_1:71142420-71142439 Msa0040880:five_prime_UTR 40.0%
GAACGGGTAAAAGAAGTTGG+TGG + chr1_1:71138394-71138413 None:intergenic 45.0%
!! ACTCTGTTTTGTTCGTGGGA+TGG + chr1_1:71139134-71139153 None:intergenic 45.0%
!! CACAGACTCTGTTTTGTTCG+TGG + chr1_1:71139139-71139158 None:intergenic 45.0%
GCAATTGAGTTAAGCTCACG+AGG - chr1_1:71139848-71139867 Msa0040880:intron 45.0%
TTCGATTCTAATGGCCGCAA+AGG + chr1_1:71140055-71140074 None:intergenic 45.0%
CCTTTCGGAGAACATGTAGT+AGG + chr1_1:71140463-71140482 None:intergenic 45.0%
CCTACTACATGTTCTCCGAA+AGG - chr1_1:71140460-71140479 Msa0040880:intron 45.0%
GGAAGGGAAATGGTACCTTT+CGG + chr1_1:71140478-71140497 None:intergenic 45.0%
! AGGTCGGTAACACTTTGGAA+GGG + chr1_1:71140494-71140513 None:intergenic 45.0%
!! ACCGATTGATTTGCCAACTC+AGG - chr1_1:71140702-71140721 Msa0040880:intron 45.0%
GTTTGACCGTTATGGCACAT+TGG - chr1_1:71140802-71140821 Msa0040880:intron 45.0%
! AATTTGTTTGTCCTGGTCCC+TGG - chr1_1:71141056-71141075 Msa0040880:intron 45.0%
GTTAGGATCGAGCAAGTCAA+CGG - chr1_1:71141338-71141357 Msa0040880:intron 45.0%
! GTACAAACCGGTGCATGTTT+TGG + chr1_1:71141679-71141698 None:intergenic 45.0%
TTTATGCACCGTGATAGAGG+GGG - chr1_1:71141993-71142012 Msa0040880:intron 45.0%
AGGGGGGAGGAATATTTCTT+TGG - chr1_1:71142010-71142029 Msa0040880:intron 45.0%
!! GACATAGATAGTGGAGTTGG+AGG - chr1_1:71142046-71142065 Msa0040880:intron 45.0%
GTAGGTAGCTCTCATAGTCA+TGG + chr1_1:71142255-71142274 None:intergenic 45.0%
!!! TTTTTCATTTTTATATTATT+TGG - chr1_1:71139107-71139126 Msa0040880:intron 5.0%
!! ATATAAATGAATATAAATTA+TGG - chr1_1:71139262-71139281 Msa0040880:intron 5.0%
!! TTAAAATATAGATTATATTT+AGG + chr1_1:71139313-71139332 None:intergenic 5.0%
!! ATAAATGTAAATTAATTAAA+CGG - chr1_1:71139996-71140015 Msa0040880:intron 5.0%
!! TTAAAAAGATTATAAATAAA+GGG + chr1_1:71140122-71140141 None:intergenic 5.0%
!! ATTAAAAAGATTATAAATAA+AGG + chr1_1:71140123-71140142 None:intergenic 5.0%
!! ATATTTCATTTATTTATTTA+AGG - chr1_1:71140290-71140309 Msa0040880:intron 5.0%
GCTGGGATCCTTTCCTAGAT+AGG + chr1_1:71140384-71140403 None:intergenic 50.0%
ATTTCTATCACCCCCACGCT+GGG + chr1_1:71140401-71140420 None:intergenic 50.0%
GGGGGTGATAGAAATGTGAG+TGG - chr1_1:71140406-71140425 Msa0040880:CDS 50.0%
GAGGTCGGTAACACTTTGGA+AGG + chr1_1:71140495-71140514 None:intergenic 50.0%
GAACGAGGTCGGTAACACTT+TGG + chr1_1:71140499-71140518 None:intergenic 50.0%
TACTTGTGTCAGGCCAAGGT+TGG - chr1_1:71140875-71140894 Msa0040880:intron 50.0%
GAATCAGTGGACACCAACCT+TGG + chr1_1:71140891-71140910 None:intergenic 50.0%
GTACATGTGGCCCCAAAGTA+TGG + chr1_1:71141218-71141237 None:intergenic 50.0%
!!! CCGGTGCATGTTTTGGTTGA+CGG + chr1_1:71141672-71141691 None:intergenic 50.0%
CCGTCAACCAAAACATGCAC+CGG - chr1_1:71141669-71141688 Msa0040880:intron 50.0%
TTATGCACCGTGATAGAGGG+GGG - chr1_1:71141994-71142013 Msa0040880:intron 50.0%
TATTCCTCCCCCCTCTATCA+CGG + chr1_1:71142004-71142023 None:intergenic 50.0%
AGTTGCAGCGGAGCCTATCT+AGG - chr1_1:71140368-71140387 Msa0040880:CDS 55.0%
CAGCGGAGCCTATCTAGGAA+AGG - chr1_1:71140373-71140392 Msa0040880:CDS 55.0%
TCTAGGAAAGGATCCCAGCG+TGG - chr1_1:71140385-71140404 Msa0040880:CDS 55.0%
CTAGGAAAGGATCCCAGCGT+GGG - chr1_1:71140386-71140405 Msa0040880:CDS 55.0%
TAGGAAAGGATCCCAGCGTG+GGG - chr1_1:71140387-71140406 Msa0040880:CDS 55.0%
CATTTCTATCACCCCCACGC+TGG + chr1_1:71140402-71140421 None:intergenic 55.0%
CGCCAACCTGTACTTGTGTC+AGG - chr1_1:71140865-71140884 Msa0040880:intron 55.0%
GGCCTGACACAAGTACAGGT+TGG + chr1_1:71140870-71140889 None:intergenic 55.0%
CCTTGGCCTGACACAAGTAC+AGG + chr1_1:71140874-71140893 None:intergenic 55.0%
CCTGTACTTGTGTCAGGCCA+AGG - chr1_1:71140871-71140890 Msa0040880:intron 55.0%
TTGTTTGTCCTGGTCCCTGG+TGG - chr1_1:71141059-71141078 Msa0040880:intron 55.0%
ATCTTCAACCACCAGGGACC+AGG + chr1_1:71141070-71141089 None:intergenic 55.0%
AGGATCGAGCAAGTCAACGG+TGG - chr1_1:71141341-71141360 Msa0040880:intron 55.0%
GAGCAAGTCAACGGTGGTAC+CGG - chr1_1:71141347-71141366 Msa0040880:intron 55.0%
!! AAGTCAACGGTGGTACCGGT+GGG - chr1_1:71141351-71141370 Msa0040880:intron 55.0%
GAATTCTGTGTGGACCCCAC+CGG + chr1_1:71141369-71141388 None:intergenic 55.0%
AGGAAAGGATCCCAGCGTGG+GGG - chr1_1:71140388-71140407 Msa0040880:CDS 60.0%
! CAAGTCAACGGTGGTACCGG+TGG - chr1_1:71141350-71141369 Msa0040880:intron 60.0%
!! AGTCAACGGTGGTACCGGTG+GGG - chr1_1:71141352-71141371 Msa0040880:intron 60.0%
TGCACCGTGATAGAGGGGGG+AGG - chr1_1:71141997-71142016 Msa0040880:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 71138303 71142522 71138303 ID=Msa0040880;Name=Msa0040880
chr1_1 mRNA 71138303 71142522 71138303 ID=Msa0040880-mRNA-1;Parent=Msa0040880;Name=Msa0040880-mRNA-1;_AED=0.18;_eAED=0.18;_QI=311|1|1|1|0.8|0.66|6|699|215
chr1_1 exon 71138303 71139089 71138303 ID=Msa0040880-mRNA-1:exon:23545;Parent=Msa0040880-mRNA-1
chr1_1 exon 71139275 71139494 71139275 ID=Msa0040880-mRNA-1:exon:23544;Parent=Msa0040880-mRNA-1
chr1_1 exon 71140344 71140455 71140344 ID=Msa0040880-mRNA-1:exon:23543;Parent=Msa0040880-mRNA-1
chr1_1 exon 71141774 71141965 71141774 ID=Msa0040880-mRNA-1:exon:23542;Parent=Msa0040880-mRNA-1
chr1_1 exon 71142079 71142221 71142079 ID=Msa0040880-mRNA-1:exon:23541;Parent=Msa0040880-mRNA-1
chr1_1 exon 71142319 71142522 71142319 ID=Msa0040880-mRNA-1:exon:23540;Parent=Msa0040880-mRNA-1
chr1_1 five_prime_UTR 71142319 71142522 71142319 ID=Msa0040880-mRNA-1:five_prime_utr;Parent=Msa0040880-mRNA-1
chr1_1 five_prime_UTR 71142115 71142221 71142115 ID=Msa0040880-mRNA-1:five_prime_utr;Parent=Msa0040880-mRNA-1
chr1_1 CDS 71142079 71142114 71142079 ID=Msa0040880-mRNA-1:cds;Parent=Msa0040880-mRNA-1
chr1_1 CDS 71141774 71141965 71141774 ID=Msa0040880-mRNA-1:cds;Parent=Msa0040880-mRNA-1
chr1_1 CDS 71140344 71140455 71140344 ID=Msa0040880-mRNA-1:cds;Parent=Msa0040880-mRNA-1
chr1_1 CDS 71139275 71139494 71139275 ID=Msa0040880-mRNA-1:cds;Parent=Msa0040880-mRNA-1
chr1_1 CDS 71139002 71139089 71139002 ID=Msa0040880-mRNA-1:cds;Parent=Msa0040880-mRNA-1
chr1_1 three_prime_UTR 71138303 71139001 71138303 ID=Msa0040880-mRNA-1:three_prime_utr;Parent=Msa0040880-mRNA-1
Gene Sequence

>Msa0040880

ATGAAAGTGATGGAACTTGCTCAAATTCCTGAGTTGGATTTGGTTTCTTCTTCTTATGAGGCTTTGGAATCAGAACAGATGAAGATAGGTTCACAGAGAATCTTGGTCTCTGATCACATAAACGCATATCAATATTCTTCTGAGAAAGTAGATAGCTTTGTGATTGATATGGACTCTTTCTCTTCTACCATCAACAAAGACATCATCAATGCCAATTCAAGAACCACGTTGCAGCGGAGCCTATCTAGGAAAGGATCCCAGCGTGGGGGTGATAGAAATGTGAGTGGCAATGTCACACTTCAAGACAAGGATATTGTCCCTACTACATGTTCTCCGAAAGCTTCTCTGTTAGGATCGAGCAAGTCAACGGTGGTACCGGTGGGGTCCACACAGAATTCAACAAATCCACAACAAGTACATCATCAAATTACAGTCACAACAGCTAGTAACATGTGTAACACCAACACAGAAAACAAAAGTATAACACGGAGAAGTAGTTTTGGACGATCTTCTTCATGGCTTCTTGACCCTAAAAAAGTTCTTCTTGTGTTTGCCACATTGTCAAGTGTAGGAACCTTGTTACTAATATTCTTGACTCTTAGTATCAGCAAGCATAATGGAGTTGAACATGGAAGTGATTTGCAATAG
Protein sequence

>Msa0040880

MKVMELAQIPELDLVSSSYEALESEQMKIGSQRILVSDHINAYQYSSEKVDSFVIDMDSFSSTINKDIINANSRTTLQRSLSRKGSQRGGDRNVSGNVTLQDKDIVPTTCSPKASLLGSSKSTVVPVGSTQNSTNPQQVHHQITVTTASNMCNTNTENKSITRRSSFGRSSSWLLDPKKVLLVFATLSSVGTLLLIFLTLSISKHNGVEHGSDLQ*