AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0135490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0135490 MtrunA17_Chr1g0206151 98.095 210 4 0 1 210 1 210 2.30e-151 418
Msa0135490 MtrunA17_Chr5g0394061 50.000 216 85 9 6 206 1 208 4.91e-54 172
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0135490 AT4G21215.2 42.529 174 78 9 51 206 23 192 2.30e-25 98.6
Msa0135490 AT4G21215.1 41.379 174 78 10 51 206 23 190 6.96e-22 89.4

Find 78 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTGAGAAATTTCGTCTTTA+AGG 0.044988 1_3:+67158460 None:intergenic
TCAGAATCAGTTACCATTTC+TGG 0.116971 1_3:-67154861 Msa0135490:three_prime_UTR
TCATTTCTCTTAGTTTAATT+TGG 0.166244 1_3:-67154164 Msa0135490:intron
TTTCTTTGGTGTGTTATATT+AGG 0.194196 1_3:-67154779 Msa0135490:three_prime_UTR
TACCGCATAAGATGAGATTT+TGG 0.242665 1_3:-67154959 Msa0135490:three_prime_UTR
TTCTTCTTCTTATGAGGCTT+TGG 0.277408 1_3:-67158065 Msa0135490:CDS
CACTTTCATAGAGGAAATAA+AGG 0.294942 1_3:+67158236 None:intergenic
TCACTTGTATCATTATATTC+TGG 0.332559 1_3:-67154687 Msa0135490:three_prime_UTR
AGATAGCTTTGTGATTGATA+TGG 0.346001 1_3:-67157960 Msa0135490:CDS
TTTGGTTTCTTCTTCTTATG+AGG 0.360374 1_3:-67158071 Msa0135490:CDS
ATTGATGATGTCTTTGTTGA+TGG 0.364743 1_3:+67157922 None:intergenic
TGAACTGTAGCTTCTCTGTT+AGG 0.382808 1_3:-67155454 Msa0135490:intron
CTTAGTATCAGCAAGCATAA+TGG 0.414607 1_3:-67155024 Msa0135490:CDS
ATAACACGGAGAAGTAGTTT+TGG 0.422585 1_3:-67155304 Msa0135490:CDS
GCCTCTTGTATCTCCAGAAA+TGG 0.422776 1_3:+67154848 None:intergenic
GAATCAGAACAGATGAAGAT+AGG 0.422813 1_3:-67158043 Msa0135490:CDS
TTTAATTTGGTAAATTTATG+TGG 0.442531 1_3:-67154151 Msa0135490:three_prime_UTR
CTTTGTGATGAAAATGATGA+TGG 0.446785 1_3:+67158571 None:intergenic
GACATAGATAGTGGAGTTGG+AGG 0.447412 1_3:-67154757 Msa0135490:three_prime_UTR
GTCAAAAGTTGCGTATTGAA+TGG 0.448696 1_3:+67158309 None:intergenic
AGTTGCAGCGGAGCCTATCT+AGG 0.453550 1_3:-67156404 Msa0135490:CDS
GCTGGGATCCTTTCCTAGAT+AGG 0.460020 1_3:+67156391 None:intergenic
TAGGACATAGATAGTGGAGT+TGG 0.468092 1_3:-67154760 Msa0135490:three_prime_UTR
CCTTTCGGAGAACATGTAGT+AGG 0.468709 1_3:+67156312 None:intergenic
ATGCACACACCTAATTGATT+CGG 0.475856 1_3:+67154373 None:intergenic
AAGTGAAGATAATAAAGAAA+AGG 0.477462 1_3:-67154602 Msa0135490:three_prime_UTR
TAAAGAAAAGGAGAGGAGAA+AGG 0.491721 1_3:-67154590 Msa0135490:three_prime_UTR
AATGATGATGGAAGTGGAAG+TGG 0.492752 1_3:+67158583 None:intergenic
ATTAAGATTCTTACCAACTC+AGG 0.492775 1_3:+67158195 None:intergenic
TATTTCCTCTATGAAAGTGA+TGG 0.495406 1_3:-67158232 Msa0135490:exon
TCAAAAGTTGCGTATTGAAT+GGG 0.502636 1_3:+67158310 None:intergenic
GATAGGTTCACAGAGAATCT+TGG 0.506776 1_3:-67158026 Msa0135490:CDS
TTATATTAGGACATAGATAG+TGG 0.508507 1_3:-67154766 Msa0135490:three_prime_UTR
GATACAAGAGGCAAATACTT+TGG 0.509461 1_3:-67154837 Msa0135490:three_prime_UTR
ACTTGCTCAAATTCCTGAGT+TGG 0.513985 1_3:-67158208 Msa0135490:intron
TAGAAGACAGAGGAAAGAAC+GGG 0.514815 1_3:+67158526 None:intergenic
ATTTGATGATGTACTTGTTG+TGG 0.518420 1_3:+67155379 None:intergenic
GAGCAAGTCAACGGTGGTAC+CGG 0.521472 1_3:-67155428 Msa0135490:CDS
AAAGAACGGGTAAAAGAAGT+TGG 0.522934 1_3:+67158539 None:intergenic
ACTTTCATAGAGGAAATAAA+GGG 0.530134 1_3:+67158237 None:intergenic
CTTAAAGACGAAATTTCTCA+CGG 0.543342 1_3:-67158459 Msa0135490:five_prime_UTR
CAAGTCAACGGTGGTACCGG+TGG 0.544727 1_3:-67155425 Msa0135490:CDS
TGATTCTGACATAAAGTTGG+TGG 0.553291 1_3:+67154875 None:intergenic
GATGAAAATGATGATGGAAG+TGG 0.559540 1_3:+67158577 None:intergenic
AAGTTCCATCACTTTCATAG+AGG 0.561165 1_3:+67158227 None:intergenic
AAGTCAACGGTGGTACCGGT+GGG 0.567710 1_3:-67155424 Msa0135490:CDS
AAGATAATAAAGAAAAGGAG+AGG 0.568001 1_3:-67154597 Msa0135490:three_prime_UTR
CTAGGAAAGGATCCCAGCGT+GGG 0.587709 1_3:-67156386 Msa0135490:CDS
AACTGATTCTGACATAAAGT+TGG 0.596741 1_3:+67154872 None:intergenic
GTAGGTAGCTCTCATAGTCA+TGG 0.598179 1_3:+67154543 None:intergenic
TCATCTTATGCGGTATAAGT+CGG 0.599570 1_3:+67154967 None:intergenic
GTAGAAGACAGAGGAAAGAA+CGG 0.600708 1_3:+67158525 None:intergenic
CTTTCGGAGAACATGTAGTA+GGG 0.607457 1_3:+67156313 None:intergenic
ACCATTTCTGGAGATACAAG+AGG 0.610878 1_3:-67154849 Msa0135490:three_prime_UTR
TAAACGATTTATAGTTGCAG+CGG 0.611666 1_3:-67156416 Msa0135490:intron
TTAAAGACGAAATTTCTCAC+GGG 0.613296 1_3:-67158458 Msa0135490:five_prime_UTR
CAGCGGAGCCTATCTAGGAA+AGG 0.618328 1_3:-67156399 Msa0135490:CDS
GAACGGGTAAAAGAAGTTGG+TGG 0.619369 1_3:+67158542 None:intergenic
TGGATTTGTTGAATTCTGTG+TGG 0.627718 1_3:+67155399 None:intergenic
CAATGTCACACTTCAAGACA+AGG 0.628034 1_3:-67156343 Msa0135490:CDS
AGTGAGGTATATAAATCCTA+CGG 0.630190 1_3:+67154705 None:intergenic
GAATTCTGTGTGGACCCCAC+CGG 0.630870 1_3:+67155409 None:intergenic
AAGCATAATGGAGTTGAACA+TGG 0.631540 1_3:-67155012 Msa0135490:CDS
TTTATGCACCGTGATAGAGG+GGG 0.635330 1_3:-67154810 Msa0135490:three_prime_UTR
GTTAGGATCGAGCAAGTCAA+CGG 0.638158 1_3:-67155437 Msa0135490:CDS
TAGGAAAGGATCCCAGCGTG+GGG 0.640099 1_3:-67156385 Msa0135490:CDS
TCTAGGAAAGGATCCCAGCG+TGG 0.641016 1_3:-67156387 Msa0135490:CDS
AGTCAACGGTGGTACCGGTG+GGG 0.643482 1_3:-67155423 Msa0135490:CDS
TGCCAAAATCTCATCTTATG+CGG 0.644341 1_3:+67154957 None:intergenic
CAATGCTAATTCAAGAACCA+CGG 0.649831 1_3:-67157903 Msa0135490:intron
CAGAAAACAAAAGTATAACA+CGG 0.658180 1_3:-67155318 Msa0135490:CDS
AGAATATAATGATACAAGTG+AGG 0.662698 1_3:+67154689 None:intergenic
CCTACTACATGTTCTCCGAA+AGG 0.663390 1_3:-67156312 Msa0135490:intron
TTATGCACCGTGATAGAGGG+GGG 0.663865 1_3:-67154809 Msa0135490:three_prime_UTR
GCAAAGTTTGTAGAAGACAG+AGG 0.666959 1_3:+67158516 None:intergenic
AGGAAAGGATCCCAGCGTGG+GGG 0.705657 1_3:-67156384 Msa0135490:CDS
ATGTGACAGAAAGTGTGAAG+AGG 0.709402 1_3:-67154935 Msa0135490:three_prime_UTR
AGGATCGAGCAAGTCAACGG+TGG 0.738297 1_3:-67155434 Msa0135490:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTATTTATTTATTTA+AGG - chr1_3:67156247-67156266 Msa0135490:intron 0.0%
!!! TTGTTCATTTTTATATTATT+TGG - chr1_3:67154903-67154922 Msa0135490:three_prime_UTR 10.0%
!!! GATAATTTTTTTTTTTTTTG+TGG - chr1_3:67155120-67155139 Msa0135490:intron 10.0%
!! GTATAAATAAATATAAATTG+TGG - chr1_3:67155220-67155239 Msa0135490:intron 10.0%
!!! ATCTATATTTTAACATAATA+TGG - chr1_3:67155279-67155298 Msa0135490:CDS 10.0%
!!! AAATTAACAACAAATTTTTT+TGG + chr1_3:67155379-67155398 None:intergenic 10.0%
!!! AAAAAAATTTGTTGTTAATT+TGG - chr1_3:67155378-67155397 Msa0135490:CDS 10.0%
!! TATATATAATAAAAGAAAAG+TGG + chr1_3:67155934-67155953 None:intergenic 10.0%
!! ATTTAATTAAACGGATAATA+AGG - chr1_3:67155963-67155982 Msa0135490:intron 15.0%
!! AAATATAATTAAAGGGTTAA+AGG + chr1_3:67156073-67156092 None:intergenic 15.0%
!!! ATTTGTTTCTTTTCATTTAT+TGG - chr1_3:67156497-67156516 Msa0135490:intron 15.0%
!!! TTTGTTTCTTTTCATTTATT+GGG - chr1_3:67156498-67156517 Msa0135490:intron 15.0%
!! AAAACACAACAAAATATATT+TGG + chr1_3:67156618-67156637 None:intergenic 15.0%
!! TTTATATTATCTTATGAATC+TGG - chr1_3:67156635-67156654 Msa0135490:intron 15.0%
!!! ATATGCAATTAATATGTTTT+TGG - chr1_3:67157142-67157161 Msa0135490:intron 15.0%
!! AATACTTTGTATATTGAATA+TGG - chr1_3:67157245-67157264 Msa0135490:intron 15.0%
!! ATACTTTGTATATTGAATAT+GGG - chr1_3:67157246-67157265 Msa0135490:intron 15.0%
!! TAAGTGTATATTTAGATTAA+CGG - chr1_3:67157546-67157565 Msa0135490:intron 15.0%
!! AAAAAATAAAATTGTGTCAA+AGG + chr1_3:67157641-67157660 None:intergenic 15.0%
!!! ACAATTTTATTTTTTGAAAC+AGG - chr1_3:67157646-67157665 Msa0135490:intron 15.0%
!! TTTACAAAAATGGAAAAAAA+AGG + chr1_3:67158102-67158121 None:intergenic 15.0%
!!! CACTTTAAAATTTACAAAAA+TGG + chr1_3:67158112-67158131 None:intergenic 15.0%
!!! TTTAATTTGGTAAATTTATG+TGG - chr1_3:67158578-67158597 Msa0135490:five_prime_UTR 15.0%
!!! TGAATTTTCAATTTTCATTC+AGG - chr1_3:67154382-67154401 Msa0135490:three_prime_UTR 20.0%
!! ATATATAAGAGAAAAAAGTG+TGG + chr1_3:67154472-67154491 None:intergenic 20.0%
!! GATTTATCAAAGAATATTGA+AGG + chr1_3:67154600-67154619 None:intergenic 20.0%
!!! TAATAAGAGTTGTGTTATTT+TGG + chr1_3:67155548-67155567 None:intergenic 20.0%
!! TGTCATAGTTTACTAAAAAA+AGG + chr1_3:67155787-67155806 None:intergenic 20.0%
!! CATACACTAAATATCAAAAA+AGG - chr1_3:67155822-67155841 Msa0135490:intron 20.0%
!!! ATATTTGTTGTTCTTTCTTT+TGG - chr1_3:67156212-67156231 Msa0135490:intron 20.0%
!!! CTAAACTATAGTCTTAATAT+TGG + chr1_3:67156905-67156924 None:intergenic 20.0%
!! CAATATTAAGACTATAGTTT+AGG - chr1_3:67156903-67156922 Msa0135490:intron 20.0%
!! GAAGAGGAAAAAATTATATA+AGG - chr1_3:67157810-67157829 Msa0135490:intron 20.0%
!! AAGTGAAGATAATAAAGAAA+AGG - chr1_3:67158127-67158146 Msa0135490:intron 20.0%
!!! TTTATTTTCTCTCTTTTCAT+TGG - chr1_3:67158438-67158457 Msa0135490:intron 20.0%
!! TCATTTCTCTTAGTTTAATT+TGG - chr1_3:67158565-67158584 Msa0135490:five_prime_UTR 20.0%
!!! CTTTTTCTTTTGGTTTTTGT+TGG - chr1_3:67154296-67154315 Msa0135490:intron 25.0%
! ACTTTCATAGAGGAAATAAA+GGG + chr1_3:67154495-67154514 None:intergenic 25.0%
! TTCCATTGAATTTGAATTCT+GGG - chr1_3:67154574-67154593 Msa0135490:three_prime_UTR 25.0%
!!! TGTGTTTTTGTGTTTTTGAT+AGG - chr1_3:67154634-67154653 Msa0135490:three_prime_UTR 25.0%
!!! TTTGTGTTTTTGATAGGATT+TGG - chr1_3:67154640-67154659 Msa0135490:three_prime_UTR 25.0%
!!! TTTTTTTTTTTTGTGGTGAC+AGG - chr1_3:67155127-67155146 Msa0135490:intron 25.0%
!!! TTTTTTTTTTTGTGGTGACA+GGG - chr1_3:67155128-67155147 Msa0135490:intron 25.0%
! AACAATGCATAATATATGCA+AGG + chr1_3:67155168-67155187 None:intergenic 25.0%
! CTAAAATCACAGTTATAAAG+TGG + chr1_3:67155625-67155644 None:intergenic 25.0%
!! TCTTTTGTACCAAAAAATGT+TGG - chr1_3:67155707-67155726 Msa0135490:intron 25.0%
! CATAGTTTACTAAAAAAAGG+TGG + chr1_3:67155784-67155803 None:intergenic 25.0%
! TATCAAAAAAGGAAGAATTG+TGG - chr1_3:67155833-67155852 Msa0135490:intron 25.0%
! AGACATTTAATATGTTTGAG+TGG + chr1_3:67155868-67155887 None:intergenic 25.0%
!! AAACTTTTGTTAAACCTTTG+CGG - chr1_3:67155996-67156015 Msa0135490:intron 25.0%
! AATTAAAGGGTTAAAGGAAA+AGG + chr1_3:67156067-67156086 None:intergenic 25.0%
! CACACACAAAGTTAATTAAT+TGG + chr1_3:67156130-67156149 None:intergenic 25.0%
! ATATTAAGTGCAATAACCAA+AGG + chr1_3:67156196-67156215 None:intergenic 25.0%
! CACTAGTAACTAAATTCAAA+TGG - chr1_3:67156785-67156804 Msa0135490:intron 25.0%
! TAAACCTCTATATCTTTGTT+GGG + chr1_3:67156952-67156971 None:intergenic 25.0%
! TTAAACCTCTATATCTTTGT+TGG + chr1_3:67156953-67156972 None:intergenic 25.0%
! ATTAGTTAAGGTGAAAAATC+AGG - chr1_3:67157042-67157061 Msa0135490:intron 25.0%
! AATGTCCAAATTTCTGATAA+TGG + chr1_3:67157198-67157217 None:intergenic 25.0%
!!! TATACTTTTGTTTTCTGTGT+TGG + chr1_3:67157408-67157427 None:intergenic 25.0%
!! CAGAAAACAAAAGTATAACA+CGG - chr1_3:67157411-67157430 Msa0135490:intron 25.0%
!!! ACACAAGAAGAACTTTTTTA+GGG + chr1_3:67157475-67157494 None:intergenic 25.0%
!!! AACACAAGAAGAACTTTTTT+AGG + chr1_3:67157476-67157495 None:intergenic 25.0%
!! TTTCTTTGGTGTGTTATATT+AGG - chr1_3:67157950-67157969 Msa0135490:CDS 25.0%
! TTATATTAGGACATAGATAG+TGG - chr1_3:67157963-67157982 Msa0135490:CDS 25.0%
! AGAATATAATGATACAAGTG+AGG + chr1_3:67158043-67158062 None:intergenic 25.0%
! TCACTTGTATCATTATATTC+TGG - chr1_3:67158042-67158061 Msa0135490:CDS 25.0%
! AAGATAATAAAGAAAAGGAG+AGG - chr1_3:67158132-67158151 Msa0135490:intron 25.0%
!! GTATTTAAAGTTGTCCAAAA+TGG + chr1_3:67158414-67158433 None:intergenic 25.0%
CTTTGTGATGAAAATGATGA+TGG + chr1_3:67154161-67154180 None:intergenic 30.0%
CTTAAAGACGAAATTTCTCA+CGG - chr1_3:67154270-67154289 Msa0135490:intron 30.0%
TTAAAGACGAAATTTCTCAC+GGG - chr1_3:67154271-67154290 Msa0135490:intron 30.0%
TCAAAAGTTGCGTATTGAAT+GGG + chr1_3:67154422-67154441 None:intergenic 30.0%
CACTTTCATAGAGGAAATAA+AGG + chr1_3:67154496-67154515 None:intergenic 30.0%
TATTTCCTCTATGAAAGTGA+TGG - chr1_3:67154497-67154516 Msa0135490:three_prime_UTR 30.0%
ATTAAGATTCTTACCAACTC+AGG + chr1_3:67154537-67154556 None:intergenic 30.0%
GTTCCATTGAATTTGAATTC+TGG - chr1_3:67154573-67154592 Msa0135490:three_prime_UTR 30.0%
! TTTGGTTTCTTCTTCTTATG+AGG - chr1_3:67154658-67154677 Msa0135490:three_prime_UTR 30.0%
!! AGATAGCTTTGTGATTGATA+TGG - chr1_3:67154769-67154788 Msa0135490:three_prime_UTR 30.0%
!! ATTGATGATGTCTTTGTTGA+TGG + chr1_3:67154810-67154829 None:intergenic 30.0%
AAGAATAAAAGTTCTTACCG+TGG + chr1_3:67154846-67154865 None:intergenic 30.0%
!!! TTTTTTTTTTGTGGTGACAG+GGG - chr1_3:67155129-67155148 Msa0135490:intron 30.0%
!!! TAAGAGAGTCCAACATTTTT+TGG + chr1_3:67155719-67155738 None:intergenic 30.0%
!!! TTACACTATGACCACTTTTT+TGG - chr1_3:67155753-67155772 Msa0135490:intron 30.0%
AGAAAGACATTCGATTCTAA+TGG + chr1_3:67156022-67156041 None:intergenic 30.0%
AACAACTTAAGAATGCAACA+TGG + chr1_3:67156169-67156188 None:intergenic 30.0%
! TCTTAAGTTGTTCTTTCCTT+TGG - chr1_3:67156177-67156196 Msa0135490:intron 30.0%
TAAACGATTTATAGTTGCAG+CGG - chr1_3:67156313-67156332 Msa0135490:CDS 30.0%
TTAGAAGAAAACAATGAACG+AGG + chr1_3:67156471-67156490 None:intergenic 30.0%
! TCTTTTCATTTATTGGGACT+TGG - chr1_3:67156504-67156523 Msa0135490:intron 30.0%
!!! TTTGTTTTTGATGTGACACA+TGG - chr1_3:67156540-67156559 Msa0135490:intron 30.0%
ACATCAAACATGATTACACA+TGG - chr1_3:67156978-67156997 Msa0135490:intron 30.0%
! TTAGTGAAATTTGTTTGTCC+TGG - chr1_3:67157003-67157022 Msa0135490:intron 30.0%
! GTTTGACAATTTTACGTAGT+AGG + chr1_3:67157071-67157090 None:intergenic 30.0%
!! TTTTTGGTACACCATACTTT+GGG - chr1_3:67157158-67157177 Msa0135490:intron 30.0%
ACTGTCCATTATCAGAAATT+TGG - chr1_3:67157190-67157209 Msa0135490:intron 30.0%
!! ATTTGATGATGTACTTGTTG+TGG + chr1_3:67157353-67157372 None:intergenic 30.0%
AAGATTTGAGTCTTACAATG+TGG + chr1_3:67157501-67157520 None:intergenic 30.0%
! AACTGATTCTGACATAAAGT+TGG + chr1_3:67157860-67157879 None:intergenic 30.0%
!!! TTTTTACTTTTTCTCTCCGT+AGG - chr1_3:67158008-67158027 Msa0135490:CDS 30.0%
AGTGAGGTATATAAATCCTA+CGG + chr1_3:67158027-67158046 None:intergenic 30.0%
AAAAAGGTTCAAATGCTAGT+AGG + chr1_3:67158086-67158105 None:intergenic 30.0%
!! AACCCACTAGTTTATTTTTC+AGG - chr1_3:67158301-67158320 Msa0135490:five_prime_UTR 30.0%
!! ACCCACTAGTTTATTTTTCA+GGG - chr1_3:67158302-67158321 Msa0135490:five_prime_UTR 30.0%
TCCCTGAAAAATAAACTAGT+GGG + chr1_3:67158306-67158325 None:intergenic 30.0%
! GAAACTTTTCCGAATCAATT+AGG - chr1_3:67158347-67158366 Msa0135490:intron 30.0%
! TGATACATAGACAACCATTT+TGG - chr1_3:67158397-67158416 Msa0135490:intron 30.0%
! TTTTCATTGGTCCAAAACAA+TGG - chr1_3:67158451-67158470 Msa0135490:five_prime_UTR 30.0%
! TTTTTCTCTCTCCATTGTTT+TGG + chr1_3:67158465-67158484 None:intergenic 30.0%
AAAACAATGGAGAGAGAAAA+AGG - chr1_3:67158464-67158483 Msa0135490:five_prime_UTR 30.0%
GTCAAAAAGTTGTCAAAAAG+TGG - chr1_3:67158531-67158550 Msa0135490:five_prime_UTR 30.0%
!! GATGAAAATGATGATGGAAG+TGG + chr1_3:67154155-67154174 None:intergenic 35.0%
AAAGAACGGGTAAAAGAAGT+TGG + chr1_3:67154193-67154212 None:intergenic 35.0%
!!! TCGTCTTTAAGGAGTGTTTT+TGG + chr1_3:67154261-67154280 None:intergenic 35.0%
CGTGAGAAATTTCGTCTTTA+AGG + chr1_3:67154272-67154291 None:intergenic 35.0%
GTCAAAAGTTGCGTATTGAA+TGG + chr1_3:67154423-67154442 None:intergenic 35.0%
AAGTTCCATCACTTTCATAG+AGG + chr1_3:67154505-67154524 None:intergenic 35.0%
TTCTTCTTCTTATGAGGCTT+TGG - chr1_3:67154664-67154683 Msa0135490:three_prime_UTR 35.0%
GAATCAGAACAGATGAAGAT+AGG - chr1_3:67154686-67154705 Msa0135490:three_prime_UTR 35.0%
CAATGCTAATTCAAGAACCA+CGG - chr1_3:67154826-67154845 Msa0135490:three_prime_UTR 35.0%
TGCATAATATATGCAAGGTC+CGG + chr1_3:67155163-67155182 None:intergenic 35.0%
ACTTGCGTGTCAAATACTAT+AGG + chr1_3:67155602-67155621 None:intergenic 35.0%
AAAGTGGACACTGTGTAATA+TGG + chr1_3:67155918-67155937 None:intergenic 35.0%
ACTTAAGAATGCAACATGGT+GGG + chr1_3:67156165-67156184 None:intergenic 35.0%
AACTTAAGAATGCAACATGG+TGG + chr1_3:67156166-67156185 None:intergenic 35.0%
AAGAAAACAATGAACGAGGT+CGG + chr1_3:67156467-67156486 None:intergenic 35.0%
CATGAAAACAAATCCTGAGT+TGG + chr1_3:67156674-67156693 None:intergenic 35.0%
CATATACTGTTTGACCGTTA+TGG - chr1_3:67156750-67156769 Msa0135490:intron 35.0%
ATATGAGCCATATGAATCAG+TGG + chr1_3:67156860-67156879 None:intergenic 35.0%
TCTATATCTTTGTTGGGTCT+GGG + chr1_3:67156946-67156965 None:intergenic 35.0%
TAACTAATCTTCAACCACCA+GGG + chr1_3:67157030-67157049 None:intergenic 35.0%
TTAACTAATCTTCAACCACC+AGG + chr1_3:67157031-67157050 None:intergenic 35.0%
! TGGTGGTTGAAGATTAGTTA+AGG - chr1_3:67157030-67157049 Msa0135490:intron 35.0%
!!! GTTTTTGGTACACCATACTT+TGG - chr1_3:67157157-67157176 Msa0135490:intron 35.0%
! TTTTGGTACACCATACTTTG+GGG - chr1_3:67157159-67157178 Msa0135490:intron 35.0%
!! TGGATTTGTTGAATTCTGTG+TGG + chr1_3:67157333-67157352 None:intergenic 35.0%
! ATAACACGGAGAAGTAGTTT+TGG - chr1_3:67157425-67157444 Msa0135490:intron 35.0%
!!! TCAGTGCATGTTTTGATTGA+CGG + chr1_3:67157607-67157626 None:intergenic 35.0%
!!! TTTTGAAACAGGTCAAGTGT+AGG - chr1_3:67157657-67157676 Msa0135490:intron 35.0%
CTTAGTATCAGCAAGCATAA+TGG - chr1_3:67157705-67157724 Msa0135490:intron 35.0%
! AAGCATAATGGAGTTGAACA+TGG - chr1_3:67157717-67157736 Msa0135490:intron 35.0%
TCATCTTATGCGGTATAAGT+CGG + chr1_3:67157765-67157784 None:intergenic 35.0%
! TACCGCATAAGATGAGATTT+TGG - chr1_3:67157770-67157789 Msa0135490:intron 35.0%
TGCCAAAATCTCATCTTATG+CGG + chr1_3:67157775-67157794 None:intergenic 35.0%
TGATTCTGACATAAAGTTGG+TGG + chr1_3:67157857-67157876 None:intergenic 35.0%
TCAGAATCAGTTACCATTTC+TGG - chr1_3:67157868-67157887 Msa0135490:intron 35.0%
GATACAAGAGGCAAATACTT+TGG - chr1_3:67157892-67157911 Msa0135490:intron 35.0%
! ATTTTATGCACCGTGATAGA+GGG - chr1_3:67157917-67157936 Msa0135490:CDS 35.0%
TAAAGAAAAGGAGAGGAGAA+AGG - chr1_3:67158139-67158158 Msa0135490:intron 35.0%
!! ACTTTCGTTTTGTAGCTTGT+AGG + chr1_3:67158207-67158226 None:intergenic 35.0%
! TGCACGCTGAATGAAATTTT+TGG - chr1_3:67158259-67158278 Msa0135490:five_prime_UTR 35.0%
GTCCCTGAAAAATAAACTAG+TGG + chr1_3:67158307-67158326 None:intergenic 35.0%
ATGCACACACCTAATTGATT+CGG + chr1_3:67158359-67158378 None:intergenic 35.0%
!! AATGATGATGGAAGTGGAAG+TGG + chr1_3:67154149-67154168 None:intergenic 40.0%
TAGAAGACAGAGGAAAGAAC+GGG + chr1_3:67154206-67154225 None:intergenic 40.0%
GTAGAAGACAGAGGAAAGAA+CGG + chr1_3:67154207-67154226 None:intergenic 40.0%
GCAAAGTTTGTAGAAGACAG+AGG + chr1_3:67154216-67154235 None:intergenic 40.0%
!!! CTCACGGGTACTTTTTCTTT+TGG - chr1_3:67154286-67154305 Msa0135490:intron 40.0%
ACTTGCTCAAATTCCTGAGT+TGG - chr1_3:67154521-67154540 Msa0135490:three_prime_UTR 40.0%
GGCCCAGAATTCAAATTCAA+TGG + chr1_3:67154579-67154598 None:intergenic 40.0%
GATAGGTTCACAGAGAATCT+TGG - chr1_3:67154703-67154722 Msa0135490:three_prime_UTR 40.0%
!! CTCTGTTTTGTTCGTGAGAT+GGG + chr1_3:67154929-67154948 None:intergenic 40.0%
!! ACTCTGTTTTGTTCGTGAGA+TGG + chr1_3:67154930-67154949 None:intergenic 40.0%
CATAATATATGCAAGGTCCG+GGG + chr1_3:67155161-67155180 None:intergenic 40.0%
GCATAATATATGCAAGGTCC+GGG + chr1_3:67155162-67155181 None:intergenic 40.0%
AGGTGGTCATACCAAAAAAG+TGG + chr1_3:67155767-67155786 None:intergenic 40.0%
TCTGCTAATGTCATCTGAGT+TGG - chr1_3:67155887-67155906 Msa0135490:intron 40.0%
CTGCTAATGTCATCTGAGTT+GGG - chr1_3:67155888-67155907 Msa0135490:intron 40.0%
CAATGTCACACTTCAAGACA+AGG - chr1_3:67156386-67156405 Msa0135490:CDS 40.0%
CTTTCGGAGAACATGTAGTA+GGG + chr1_3:67156419-67156438 None:intergenic 40.0%
! GTAACACTTTGGAAGGGAAA+TGG + chr1_3:67156445-67156464 None:intergenic 40.0%
! TCCTGAGTTGGCAAATCAAT+CGG + chr1_3:67156662-67156681 None:intergenic 40.0%
TGTTCACCAATGTGCCATAA+CGG + chr1_3:67156767-67156786 None:intergenic 40.0%
!! TTGGTGTCCACTGATTCATA+TGG - chr1_3:67156850-67156869 Msa0135490:intron 40.0%
CTCTATATCTTTGTTGGGTC+TGG + chr1_3:67156947-67156966 None:intergenic 40.0%
CAGACCCAACAAAGATATAG+AGG - chr1_3:67156945-67156964 Msa0135490:intron 40.0%
!! CTGATAATGGACAGTACATG+TGG + chr1_3:67157185-67157204 None:intergenic 40.0%
TGAACTGTAGCTTCTCTGTT+AGG - chr1_3:67157275-67157294 Msa0135490:intron 40.0%
!! GTTTTGGACGATCTTCTTCA+TGG - chr1_3:67157441-67157460 Msa0135490:intron 40.0%
! ATGTGACAGAAAGTGTGAAG+AGG - chr1_3:67157794-67157813 Msa0135490:intron 40.0%
! ACCATTTCTGGAGATACAAG+AGG - chr1_3:67157880-67157899 Msa0135490:intron 40.0%
! GATTTTATGCACCGTGATAG+AGG - chr1_3:67157916-67157935 Msa0135490:CDS 40.0%
! TTTTATGCACCGTGATAGAG+GGG - chr1_3:67157918-67157937 Msa0135490:CDS 40.0%
!! TAGGACATAGATAGTGGAGT+TGG - chr1_3:67157969-67157988 Msa0135490:CDS 40.0%
GAACGGGTAAAAGAAGTTGG+TGG + chr1_3:67154190-67154209 None:intergenic 45.0%
TCTTGTGAGCTTAGCTCAGT+TGG + chr1_3:67155195-67155214 None:intergenic 45.0%
TTCGATTCTAATGGCCGCAA+AGG + chr1_3:67156013-67156032 None:intergenic 45.0%
CCTTTCGGAGAACATGTAGT+AGG + chr1_3:67156420-67156439 None:intergenic 45.0%
CCTACTACATGTTCTCCGAA+AGG - chr1_3:67156417-67156436 Msa0135490:intron 45.0%
GGAAGGGAAATGGTACCTTT+CGG + chr1_3:67156435-67156454 None:intergenic 45.0%
! AGGTCGGTAACACTTTGGAA+GGG + chr1_3:67156451-67156470 None:intergenic 45.0%
!! ACCGATTGATTTGCCAACTC+AGG - chr1_3:67156658-67156677 Msa0135490:intron 45.0%
GTTTGACCGTTATGGCACAT+TGG - chr1_3:67156758-67156777 Msa0135490:intron 45.0%
! AATTTGTTTGTCCTGGTCCC+TGG - chr1_3:67157010-67157029 Msa0135490:intron 45.0%
GTTAGGATCGAGCAAGTCAA+CGG - chr1_3:67157292-67157311 Msa0135490:intron 45.0%
GCCTCTTGTATCTCCAGAAA+TGG + chr1_3:67157884-67157903 None:intergenic 45.0%
TTTATGCACCGTGATAGAGG+GGG - chr1_3:67157919-67157938 Msa0135490:CDS 45.0%
AGGGGGGAGGAATATTTCTT+TGG - chr1_3:67157936-67157955 Msa0135490:CDS 45.0%
!! GACATAGATAGTGGAGTTGG+AGG - chr1_3:67157972-67157991 Msa0135490:CDS 45.0%
GTAGGTAGCTCTCATAGTCA+TGG + chr1_3:67158189-67158208 None:intergenic 45.0%
GGAGAGAGAAAAAGGAAGAG+AGG - chr1_3:67158472-67158491 Msa0135490:five_prime_UTR 45.0%
!! TTAAAATATAGATTATATTT+AGG + chr1_3:67155272-67155291 None:intergenic 5.0%
!! AATATTAATATAATTTCAAT+AGG + chr1_3:67155490-67155509 None:intergenic 5.0%
!! TAAATGAAAATTTAATTAAA+CGG - chr1_3:67155954-67155973 Msa0135490:intron 5.0%
!! ATTCAAAAAATATAATTAAA+GGG + chr1_3:67156080-67156099 None:intergenic 5.0%
!! AATTCAAAAAATATAATTAA+AGG + chr1_3:67156081-67156100 None:intergenic 5.0%
GCTGGGATCCTTTCCTAGAT+AGG + chr1_3:67156341-67156360 None:intergenic 50.0%
ATTTCTATCACCCCCACGCT+GGG + chr1_3:67156358-67156377 None:intergenic 50.0%
GGGGGTGATAGAAATGTGAG+TGG - chr1_3:67156363-67156382 Msa0135490:CDS 50.0%
GAGGTCGGTAACACTTTGGA+AGG + chr1_3:67156452-67156471 None:intergenic 50.0%
GAACGAGGTCGGTAACACTT+TGG + chr1_3:67156456-67156475 None:intergenic 50.0%
TACTTGTGTCAGGCCAAGGT+TGG - chr1_3:67156831-67156850 Msa0135490:intron 50.0%
GAATCAGTGGACACCAACCT+TGG + chr1_3:67156847-67156866 None:intergenic 50.0%
GTACATGTGGCCCCAAAGTA+TGG + chr1_3:67157172-67157191 None:intergenic 50.0%
TTATGCACCGTGATAGAGGG+GGG - chr1_3:67157920-67157939 Msa0135490:CDS 50.0%
TATTCCTCCCCCCTCTATCA+CGG + chr1_3:67157930-67157949 None:intergenic 50.0%
AGTTGCAGCGGAGCCTATCT+AGG - chr1_3:67156325-67156344 Msa0135490:CDS 55.0%
CAGCGGAGCCTATCTAGGAA+AGG - chr1_3:67156330-67156349 Msa0135490:CDS 55.0%
TCTAGGAAAGGATCCCAGCG+TGG - chr1_3:67156342-67156361 Msa0135490:CDS 55.0%
CTAGGAAAGGATCCCAGCGT+GGG - chr1_3:67156343-67156362 Msa0135490:CDS 55.0%
TAGGAAAGGATCCCAGCGTG+GGG - chr1_3:67156344-67156363 Msa0135490:CDS 55.0%
CATTTCTATCACCCCCACGC+TGG + chr1_3:67156359-67156378 None:intergenic 55.0%
CGCCAACCTGTACTTGTGTC+AGG - chr1_3:67156821-67156840 Msa0135490:intron 55.0%
GGCCTGACACAAGTACAGGT+TGG + chr1_3:67156826-67156845 None:intergenic 55.0%
CCTTGGCCTGACACAAGTAC+AGG + chr1_3:67156830-67156849 None:intergenic 55.0%
CCTGTACTTGTGTCAGGCCA+AGG - chr1_3:67156827-67156846 Msa0135490:intron 55.0%
TTGTTTGTCCTGGTCCCTGG+TGG - chr1_3:67157013-67157032 Msa0135490:intron 55.0%
ATCTTCAACCACCAGGGACC+AGG + chr1_3:67157024-67157043 None:intergenic 55.0%
AGGATCGAGCAAGTCAACGG+TGG - chr1_3:67157295-67157314 Msa0135490:intron 55.0%
GAGCAAGTCAACGGTGGTAC+CGG - chr1_3:67157301-67157320 Msa0135490:intron 55.0%
!! AAGTCAACGGTGGTACCGGT+GGG - chr1_3:67157305-67157324 Msa0135490:intron 55.0%
GAATTCTGTGTGGACCCCAC+CGG + chr1_3:67157323-67157342 None:intergenic 55.0%
GGTGACAGGGGTTTGAACCC+CGG - chr1_3:67155141-67155160 Msa0135490:intron 60.0%
AGGAAAGGATCCCAGCGTGG+GGG - chr1_3:67156345-67156364 Msa0135490:CDS 60.0%
! CAAGTCAACGGTGGTACCGG+TGG - chr1_3:67157304-67157323 Msa0135490:intron 60.0%
!! AGTCAACGGTGGTACCGGTG+GGG - chr1_3:67157306-67157325 Msa0135490:intron 60.0%
TGCACCGTGATAGAGGGGGG+AGG - chr1_3:67157923-67157942 Msa0135490:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 67154103 67158648 67154103 ID=Msa0135490;Name=Msa0135490
chr1_3 mRNA 67154103 67158648 67154103 ID=Msa0135490-mRNA-1;Parent=Msa0135490;Name=Msa0135490-mRNA-1;_AED=0.06;_eAED=0.06;_QI=307|1|1|1|0.66|0.42|7|701|215
chr1_3 exon 67154103 67154173 67154103 ID=Msa0135490-mRNA-1:exon:23002;Parent=Msa0135490-mRNA-1
chr1_3 exon 67154366 67155083 67154366 ID=Msa0135490-mRNA-1:exon:23001;Parent=Msa0135490-mRNA-1
chr1_3 exon 67155247 67155466 67155247 ID=Msa0135490-mRNA-1:exon:23000;Parent=Msa0135490-mRNA-1
chr1_3 exon 67156313 67156424 67156313 ID=Msa0135490-mRNA-1:exon:22999;Parent=Msa0135490-mRNA-1
chr1_3 exon 67157904 67158095 67157904 ID=Msa0135490-mRNA-1:exon:22998;Parent=Msa0135490-mRNA-1
chr1_3 exon 67158209 67158351 67158209 ID=Msa0135490-mRNA-1:exon:22997;Parent=Msa0135490-mRNA-1
chr1_3 exon 67158449 67158648 67158449 ID=Msa0135490-mRNA-1:exon:22996;Parent=Msa0135490-mRNA-1
chr1_3 five_prime_UTR 67158449 67158648 67158449 ID=Msa0135490-mRNA-1:five_prime_utr;Parent=Msa0135490-mRNA-1
chr1_3 five_prime_UTR 67158245 67158351 67158245 ID=Msa0135490-mRNA-1:five_prime_utr;Parent=Msa0135490-mRNA-1
chr1_3 CDS 67158209 67158244 67158209 ID=Msa0135490-mRNA-1:cds;Parent=Msa0135490-mRNA-1
chr1_3 CDS 67157904 67158095 67157904 ID=Msa0135490-mRNA-1:cds;Parent=Msa0135490-mRNA-1
chr1_3 CDS 67156313 67156424 67156313 ID=Msa0135490-mRNA-1:cds;Parent=Msa0135490-mRNA-1
chr1_3 CDS 67155247 67155466 67155247 ID=Msa0135490-mRNA-1:cds;Parent=Msa0135490-mRNA-1
chr1_3 CDS 67154996 67155083 67154996 ID=Msa0135490-mRNA-1:cds;Parent=Msa0135490-mRNA-1
chr1_3 three_prime_UTR 67154366 67154995 67154366 ID=Msa0135490-mRNA-1:three_prime_utr;Parent=Msa0135490-mRNA-1
chr1_3 three_prime_UTR 67154103 67154173 67154103 ID=Msa0135490-mRNA-1:three_prime_utr;Parent=Msa0135490-mRNA-1
Gene Sequence

>Msa0135490

ATGAAAGTGATGGAACTTGCTCAAATTCCTGAGTTGGATTTGGTTTCTTCTTCTTATGAGGCTTTGGAATCAGAACAGATGAAGATAGGTTCACAGAGAATCTTGGTCTCTGATCACATAAACGCATATCAATATTCTTCTGAGAAAGTAGATAGCTTTGTGATTGATATGGACTCTTTCTCTTCTACCATCAACAAAGACATCATCAATGCTAATTCAAGAACCACGTTGCAGCGGAGCCTATCTAGGAAAGGATCCCAGCGTGGGGGTGATAGAAATGTGAGTGGCAATGTCACACTTCAAGACAAGGATATTGTCCCTACTACATGTTCTCCGAAAGCTTCTCTGTTAGGATCGAGCAAGTCAACGGTGGTACCGGTGGGGTCCACACAGAATTCAACAAATCCACAACAAGTACATCATCAAATTACAGTCACAACAGCTAGTAACATGTGTAACACCAACACAGAAAACAAAAGTATAACACGGAGAAGTAGTTTTGGACGATCTTCTTCATGGCTTCTTGACCCTAAAAAAGTTCTTCTTGTGTTTGCCACATTGTCAAGTGTAGGAACATTGTTACTAATATTCTTGACTCTTAGTATCAGCAAGCATAATGGAGTTGAACATGGAAGTGATTTGCAATAG
Protein sequence

>Msa0135490

MKVMELAQIPELDLVSSSYEALESEQMKIGSQRILVSDHINAYQYSSEKVDSFVIDMDSFSSTINKDIINANSRTTLQRSLSRKGSQRGGDRNVSGNVTLQDKDIVPTTCSPKASLLGSSKSTVVPVGSTQNSTNPQQVHHQITVTTASNMCNTNTENKSITRRSSFGRSSSWLLDPKKVLLVFATLSSVGTLLLIFLTLSISKHNGVEHGSDLQ*