AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0045410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0045410 MtrunA17_Chr1g0211291 97.021 235 7 0 13 247 200 434 9.13e-166 468
Msa0045410 MtrunA17_Chr7g0250051 96.596 235 8 0 13 247 198 432 3.34e-165 467
Msa0045410 MtrunA17_Chr4g0015651 30.270 185 126 2 15 197 188 371 1.36e-19 88.2
Msa0045410 MtrunA17_Chr4g0016741 30.270 185 126 2 15 197 188 371 1.36e-19 88.2
Msa0045410 MtrunA17_CPg0492941 30.270 185 126 2 15 197 188 371 1.36e-19 88.2
Msa0045410 MtrunA17_Chr4g0000341 32.704 159 101 2 39 192 303 460 3.00e-19 87.4
Msa0045410 MtrunA17_Chr4g0016371 29.545 220 140 4 41 252 185 397 1.00e-16 79.7
Msa0045410 MtrunA17_Chr3g0090971 29.545 220 140 4 41 252 224 436 1.65e-16 79.0
Msa0045410 MtrunA17_CPg0493401 29.545 220 140 4 41 252 224 436 1.71e-16 79.0
Msa0045410 MtrunA17_Chr7g0229391 29.224 219 140 4 42 252 225 436 7.89e-16 77.0
Msa0045410 MtrunA17_MTg0490471 28.649 185 129 2 15 197 198 381 7.93e-16 77.0
Msa0045410 MtrunA17_Chr1g0208761 26.872 227 152 3 22 240 260 480 2.04e-15 75.9
Msa0045410 MtrunA17_Chr1g0208751 26.872 227 152 3 22 240 257 477 5.24e-15 74.7
Msa0045410 MtrunA17_Chr2g0278821 29.375 160 105 3 57 214 11 164 7.90e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0045410 AT4G38510.1 93.827 243 12 1 5 247 193 432 7.24e-164 464
Msa0045410 AT4G38510.3 93.827 243 12 1 5 247 193 432 7.24e-164 464
Msa0045410 AT4G38510.4 93.827 243 12 1 5 247 193 432 7.24e-164 464
Msa0045410 AT4G38510.2 93.827 243 12 1 5 247 193 432 7.24e-164 464
Msa0045410 AT4G38510.5 93.827 243 12 1 5 247 200 439 8.77e-164 464
Msa0045410 AT1G20260.1 93.416 243 14 1 5 247 192 432 5.58e-163 461
Msa0045410 AT1G76030.1 96.552 232 8 0 16 247 200 431 1.56e-162 460
Msa0045410 ATCG00120.1 29.730 185 127 2 15 197 188 371 1.95e-19 88.2
Msa0045410 AT1G78900.1 32.704 159 101 2 39 192 302 459 3.27e-19 87.4
Msa0045410 AT1G78900.2 32.704 159 101 2 39 192 302 459 3.27e-19 87.4
Msa0045410 ATCG00480.1 29.224 219 142 3 41 252 224 436 1.43e-17 82.4
Msa0045410 AT2G07698.1 29.189 185 128 2 15 197 468 651 1.75e-16 79.7
Msa0045410 ATMG01190.1 29.189 185 128 2 15 197 198 381 2.44e-16 79.0
Msa0045410 AT5G08670.1 27.632 228 149 4 22 240 257 477 9.00e-16 77.4
Msa0045410 AT5G08690.1 27.632 228 149 4 22 240 257 477 9.69e-16 77.4
Msa0045410 AT5G08680.1 28.384 229 146 5 22 240 260 480 1.02e-15 77.0

Find 107 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGGTTAGAATTGGAATT+TGG 0.112862 1_1:+77341712 None:intergenic
ACGGTTGTATCTGGAATTTC+TGG 0.155234 1_1:-77340431 Msa0045410:three_prime_UTR
ATCTTGTAATTTAATTATTT+AGG 0.241526 1_1:-77339914 Msa0045410:three_prime_UTR
TATTTAGGATATTTGTAATA+AGG 0.248801 1_1:-77339899 Msa0045410:three_prime_UTR
TCTTACTACTGCTGAATATC+TGG 0.262357 1_1:-77342151 Msa0045410:CDS
ATATTATTAACGGTTGTATC+TGG 0.266315 1_1:-77340440 Msa0045410:exon
CATGGTTAGAATTGGAATTT+GGG 0.316289 1_1:+77341713 None:intergenic
CCAGGATAACCACGTCTTCC+TGG 0.316708 1_1:+77341807 None:intergenic
TCACCACTGCAGAGTGCTAT+TGG 0.329482 1_1:-77340798 Msa0045410:intron
TACTTGTGTTGCACACAGTT+TGG 0.330060 1_1:-77339998 Msa0045410:three_prime_UTR
ACATCTACGGGTTCTTCTTT+CGG 0.330542 1_1:+77340064 None:intergenic
TCATTTGGCATGGTTAGAAT+TGG 0.334129 1_1:+77341705 None:intergenic
CACCACTGCAGAGTGCTATT+GGG 0.340834 1_1:-77340797 Msa0045410:intron
CTGTCCCTCCGTAATATATC+TGG 0.348917 1_1:+77341559 None:intergenic
ATTACTTCATTACCATCATT+TGG 0.354095 1_1:+77341690 None:intergenic
CAGCATCTCGGCTGTAAAAC+TGG 0.358949 1_1:+77340272 None:intergenic
ATCCATCATAAATATAATAA+AGG 0.361171 1_1:-77340133 Msa0045410:three_prime_UTR
TGCAGGTGTCGTAGGCTCCC+TGG 0.382480 1_1:+77340383 None:intergenic
TTTGGGATGGTTGGAATTTG+TGG 0.399858 1_1:-77339980 Msa0045410:three_prime_UTR
AGATTACCAAATTAAGGAAA+AGG 0.411767 1_1:+77342799 None:intergenic
AACCTTTATTATATTTATGA+TGG 0.413732 1_1:+77340131 None:intergenic
TTTACGCCCCTCAATTCTTC+CGG 0.416790 1_1:+77341746 None:intergenic
ACTTGTGTTGCACACAGTTT+GGG 0.417897 1_1:-77339997 Msa0045410:three_prime_UTR
AATCAATTGCCCATGACATT+AGG 0.421049 1_1:+77340194 None:intergenic
CAACGACAGCTTTCATTGCC+TGG 0.422877 1_1:+77340584 None:intergenic
CTTCATTAATACCCACTTCA+AGG 0.424318 1_1:+77340476 None:intergenic
TTCTTTCGGACTAACTGAAC+AGG 0.425311 1_1:+77340078 None:intergenic
TCTTCCTGGCACCTCTTCTC+TGG 0.425819 1_1:+77341821 None:intergenic
TAGAGACTGGAATATATTGC+AGG 0.434624 1_1:+77340366 None:intergenic
TGAGAGGAAGTTTGTTGCCC+AGG 0.437811 1_1:-77340401 Msa0045410:three_prime_UTR
GATTACCAAATTAAGGAAAA+GGG 0.449091 1_1:+77342800 None:intergenic
AAGCGTGACTTTGAGGAGAA+TGG 0.451660 1_1:-77342840 Msa0045410:CDS
GAAGTACACGAGGGAAAATC+CGG 0.454247 1_1:+77340323 None:intergenic
TGATCTTACCAGATATATTA+CGG 0.458091 1_1:-77341567 Msa0045410:CDS
GTGATGATACGTTCGATTGT+AGG 0.458361 1_1:+77342186 None:intergenic
CCAGGAAGACGTGGTTATCC+TGG 0.466984 1_1:-77341807 Msa0045410:CDS
TGAACATGCCGGAAGAATTG+AGG 0.476429 1_1:-77341754 Msa0045410:CDS
TGCACACAGTTTGGGATGGT+TGG 0.495421 1_1:-77339989 Msa0045410:three_prime_UTR
GTAATATATCTGGTAAGATC+AGG 0.498299 1_1:+77341569 None:intergenic
CTGAACAGGCTCAAAATATG+AGG 0.506055 1_1:+77340092 None:intergenic
GCTGCCAGAGAAGAGGTGCC+AGG 0.506143 1_1:-77341825 Msa0045410:CDS
GAACATGCCGGAAGAATTGA+GGG 0.507410 1_1:-77341753 Msa0045410:CDS
TGAAACAAGCAAACATACAC+TGG 0.507667 1_1:+77339948 None:intergenic
CTTTGAGGAGAATGGCTCAA+TGG 0.507722 1_1:-77342832 Msa0045410:CDS
GCAGGTGTCGTAGGCTCCCT+GGG 0.511296 1_1:+77340384 None:intergenic
ATGTTCTTCCATCACTATCT+CGG 0.513623 1_1:-77340930 Msa0045410:CDS
TCCATGCAAGATCTAGAGAC+TGG 0.515422 1_1:+77340353 None:intergenic
TGCAGCTATGGGAGTTAACA+TGG 0.517732 1_1:-77342880 Msa0045410:CDS
TGCCATTGGAAAGGATGTCC+AGG 0.518353 1_1:-77340602 Msa0045410:CDS
TCAATCAGCGCCTAATGTCA+TGG 0.518643 1_1:-77340204 Msa0045410:three_prime_UTR
ATCACTATCTCGGTTGATGA+AGG 0.519694 1_1:-77340920 Msa0045410:intron
ATCTTGCATGGACTTTACTC+CGG 0.519977 1_1:-77340342 Msa0045410:three_prime_UTR
ATTCTAACCATGCCAAATGA+TGG 0.521293 1_1:-77341702 Msa0045410:intron
TATAGGAATCACAACATCTA+CGG 0.526427 1_1:+77340051 None:intergenic
GTAAAACTGGTCAAGAGTCT+TGG 0.529021 1_1:+77340285 None:intergenic
TACAACCGTTAATAATATTG+AGG 0.530534 1_1:+77340445 None:intergenic
GCTGATTGAGCAAGAGACTA+AGG 0.530727 1_1:+77340218 None:intergenic
TGGGGAGGGAATGACTAGAA+AGG 0.531100 1_1:-77340778 Msa0045410:CDS
GAATATATTGCAGGTGTCGT+AGG 0.532438 1_1:+77340375 None:intergenic
TTCATTACCATCATTTGGCA+TGG 0.532782 1_1:+77341695 None:intergenic
TAATAAGGATAGTTGTCACT+TGG 0.536391 1_1:-77339884 Msa0045410:three_prime_UTR
CTGTATGCAACTTATGCCAT+TGG 0.536747 1_1:-77340616 Msa0045410:CDS
AGCACTTTCATCTGAGGACC+TGG 0.537514 1_1:-77340554 Msa0045410:CDS
TAGGCAGTTGCATAATAGAC+AGG 0.544818 1_1:-77341525 Msa0045410:intron
TCCAGTCTCTAGATCTTGCA+TGG 0.545696 1_1:-77340354 Msa0045410:three_prime_UTR
GAGAGGAAGTTTGTTGCCCA+GGG 0.548471 1_1:-77340400 Msa0045410:three_prime_UTR
ATCATCAATTACCAGCATCT+CGG 0.552362 1_1:+77340260 None:intergenic
AGTGATGGAAGAACATTGAT+TGG 0.552550 1_1:+77340937 None:intergenic
CAATCAGCGCCTAATGTCAT+GGG 0.553748 1_1:-77340203 Msa0045410:three_prime_UTR
AACTGGTCAAGAGTCTTGGC+TGG 0.554684 1_1:+77340289 None:intergenic
GGAAGAATTGAGGGGCGTAA+AGG 0.556865 1_1:-77341744 Msa0045410:CDS
AGAGAAAATTAGTAGATGCC+AGG 0.561602 1_1:+77340536 None:intergenic
ACCACTGCAGAGTGCTATTG+GGG 0.563672 1_1:-77340796 Msa0045410:intron
ATAGGAATCACAACATCTAC+GGG 0.567475 1_1:+77340052 None:intergenic
TGCCTGGACATCCTTTCCAA+TGG 0.569487 1_1:+77340600 None:intergenic
TAGTGAAATTGTCCTTGAAG+TGG 0.569681 1_1:-77340488 Msa0045410:CDS
CTGCAGAGTGCTATTGGGGA+GGG 0.572458 1_1:-77340792 Msa0045410:intron
GCAACAATTTATGAACATGC+CGG 0.577220 1_1:-77341765 Msa0045410:CDS
CAGGCAATGAAAGCTGTCGT+TGG 0.577939 1_1:-77340583 Msa0045410:CDS
TCATTCTGATGTGTCCAACC+AGG 0.578586 1_1:-77340754 Msa0045410:intron
GATAGAACAAATGCGGACTT+AGG 0.587137 1_1:+77340153 None:intergenic
GAATATCTGGCTTATGAATG+TGG 0.590666 1_1:-77342138 Msa0045410:CDS
ACGAACAGATAAATACATCA+AGG 0.595675 1_1:+77339855 None:intergenic
AGTGAAATTGTCCTTGAAGT+GGG 0.600879 1_1:-77340487 Msa0045410:CDS
ATGGAAGAACATTGATTGGT+GGG 0.602705 1_1:+77340941 None:intergenic
GTGTTGCACACAGTTTGGGA+TGG 0.602819 1_1:-77339993 Msa0045410:three_prime_UTR
AGGGACAGATATACATCGAT+AGG 0.603945 1_1:-77341544 Msa0045410:CDS
AATTTCTGGACAAGTTTGAG+AGG 0.606081 1_1:-77340417 Msa0045410:three_prime_UTR
TATCTGGTAAGATCAGGAGT+TGG 0.610819 1_1:+77341575 None:intergenic
TTCATCAACCGAGATAGTGA+TGG 0.612897 1_1:+77340922 None:intergenic
AGAAGAAGCACTTTCATCTG+AGG 0.617772 1_1:-77340560 Msa0045410:CDS
TTATGCTGATGCTCTTCGTG+AGG 0.621125 1_1:-77342079 Msa0045410:intron
GGTATCTGCTGCCAGAGAAG+AGG 0.624465 1_1:-77341832 Msa0045410:CDS
GAAGAGGTGCCAGGAAGACG+TGG 0.628045 1_1:-77341816 Msa0045410:CDS
GATGGAAGAACATTGATTGG+TGG 0.633609 1_1:+77340940 None:intergenic
ACTGCAGAGTGCTATTGGGG+AGG 0.635578 1_1:-77340793 Msa0045410:intron
GAAACAAGCAAACATACACT+GGG 0.645433 1_1:+77339949 None:intergenic
TGCAACTTATGCCATTGGAA+AGG 0.655175 1_1:-77340611 Msa0045410:CDS
CTTACCAGATATATTACGGA+GGG 0.659583 1_1:-77341563 Msa0045410:CDS
AGATCTGTATACATATAACC+AGG 0.661413 1_1:+77341789 None:intergenic
CAAACAAGAAGAGACCTGGT+TGG 0.666933 1_1:+77340740 None:intergenic
TATGATGGATAGAACAAATG+CGG 0.680825 1_1:+77340146 None:intergenic
TCTTACCAGATATATTACGG+AGG 0.728933 1_1:-77341564 Msa0045410:CDS
ATACGATGAAGAAGTACACG+AGG 0.729276 1_1:+77340313 None:intergenic
GCAGTAGTAAGAGCAATACG+AGG 0.737884 1_1:+77342162 None:intergenic
TACGATGAAGAAGTACACGA+GGG 0.742809 1_1:+77340314 None:intergenic
AACATGCCGGAAGAATTGAG+GGG 0.764342 1_1:-77341752 Msa0045410:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATAATTTTTTTAACAAG+GGG + chr1_1:77340722-77340741 None:intergenic 10.0%
!!! CATTATAATTTTTTTAACAA+GGG + chr1_1:77340723-77340742 None:intergenic 10.0%
!!! TCATTATAATTTTTTTAACA+AGG + chr1_1:77340724-77340743 None:intergenic 10.0%
!! ATCTTGTAATTTAATTATTT+AGG - chr1_1:77343100-77343119 Msa0045410:intron 10.0%
!!! TTTTAAAGATTACCAAATTA+AGG + chr1_1:77340224-77340243 None:intergenic 15.0%
!!! AATTTTCTAAAAACGTTAAA+TGG - chr1_1:77340592-77340611 Msa0045410:CDS 15.0%
!!! TACTTCAATATTTTGTTTAA+TGG - chr1_1:77340641-77340660 Msa0045410:intron 15.0%
!!! TTATAATTTTTTTAACAAGG+GGG + chr1_1:77340721-77340740 None:intergenic 15.0%
!! GGTTAAGAAATAAAAATTAT+CGG + chr1_1:77340755-77340774 None:intergenic 15.0%
!!! AGTAAATTTTGCTTATATTT+TGG + chr1_1:77341634-77341653 None:intergenic 15.0%
!!! ATAAGCAAAATTTACTTTTT+AGG - chr1_1:77341638-77341657 Msa0045410:intron 15.0%
!!! ATTTTGCTTATATTTTGAAA+CGG - chr1_1:77341736-77341755 Msa0045410:CDS 15.0%
!! ACATATATTTCTTAATCAAA+AGG - chr1_1:77342160-77342179 Msa0045410:CDS 15.0%
!! TTATATGTAAACTATCTTAT+TGG - chr1_1:77342319-77342338 Msa0045410:intron 15.0%
!!! ATTTTCCTCAATATTATTAA+CGG - chr1_1:77342564-77342583 Msa0045410:intron 15.0%
!! ATCCATCATAAATATAATAA+AGG - chr1_1:77342881-77342900 Msa0045410:CDS 15.0%
!! AACCTTTATTATATTTATGA+TGG + chr1_1:77342886-77342905 None:intergenic 15.0%
!!! TATTTAGGATATTTGTAATA+AGG - chr1_1:77343115-77343134 Msa0045410:intron 15.0%
!! TACTCATATTACATAAAGTA+TGG + chr1_1:77340046-77340065 None:intergenic 20.0%
!! TACTTTATGTAATATGAGTA+AGG - chr1_1:77340046-77340065 Msa0045410:three_prime_UTR 20.0%
!!! TTTGATTCTTCATTTTTCTT+TGG - chr1_1:77340400-77340419 Msa0045410:three_prime_UTR 20.0%
!!! TTGATTCTTCATTTTTCTTT+GGG - chr1_1:77340401-77340420 Msa0045410:three_prime_UTR 20.0%
!! TAAGAAATAAAAATTATCGG+AGG + chr1_1:77340752-77340771 None:intergenic 20.0%
!!! TTTTCCTAATAAATTACAGT+TGG + chr1_1:77340968-77340987 None:intergenic 20.0%
!!! ATAAGCAAAATTCACTTTTT+AGG + chr1_1:77341727-77341746 None:intergenic 20.0%
!! ATATAAAAGCAGTTAATTGT+TGG - chr1_1:77341788-77341807 Msa0045410:CDS 20.0%
!! TAAAAAAAGAAAACTACACA+GGG + chr1_1:77342302-77342321 None:intergenic 20.0%
!! ATAAAAAAAGAAAACTACAC+AGG + chr1_1:77342303-77342322 None:intergenic 20.0%
! ATAATGATTAATACGTCAGT+AGG + chr1_1:77339866-77339885 None:intergenic 25.0%
! TTTATGTAATATGAGTAAGG+TGG - chr1_1:77340049-77340068 Msa0045410:three_prime_UTR 25.0%
! TTACAAATCTTGATTTATGC+AGG - chr1_1:77340078-77340097 Msa0045410:three_prime_UTR 25.0%
! TACAAATCTTGATTTATGCA+GGG - chr1_1:77340079-77340098 Msa0045410:three_prime_UTR 25.0%
! GATTACCAAATTAAGGAAAA+GGG + chr1_1:77340217-77340236 None:intergenic 25.0%
! AGATTACCAAATTAAGGAAA+AGG + chr1_1:77340218-77340237 None:intergenic 25.0%
! AACCAAAATTACCAGAATAA+GGG + chr1_1:77340273-77340292 None:intergenic 25.0%
! AAACCAAAATTACCAGAATA+AGG + chr1_1:77340274-77340293 None:intergenic 25.0%
! AATAAATGTCTCATAATGCT+TGG - chr1_1:77340353-77340372 Msa0045410:three_prime_UTR 25.0%
!!! TGATTCTTCATTTTTCTTTG+GGG - chr1_1:77340402-77340421 Msa0045410:three_prime_UTR 25.0%
!!! ATACTAGTTCTACATTAAGA+TGG + chr1_1:77340519-77340538 None:intergenic 25.0%
! CTTAATGTAGAACTAGTATT+TGG - chr1_1:77340520-77340539 Msa0045410:CDS 25.0%
! TGTACCAACTGTAATTTATT+AGG - chr1_1:77340961-77340980 Msa0045410:intron 25.0%
! TAAAGAAAAATACAGGAGAA+TGG + chr1_1:77341040-77341059 None:intergenic 25.0%
!! AATCCTCTAAAGAAAAATAC+AGG + chr1_1:77341047-77341066 None:intergenic 25.0%
! ATTACTTCATTACCATCATT+TGG + chr1_1:77341327-77341346 None:intergenic 25.0%
!! ATGAATTTTGTCTTCTGTTT+TGG - chr1_1:77341345-77341364 Msa0045410:intron 25.0%
! TGATCTTACCAGATATATTA+CGG - chr1_1:77341447-77341466 Msa0045410:intron 25.0%
! CATTCATTTAATGATGTATG+TGG - chr1_1:77341663-77341682 Msa0045410:intron 25.0%
! CATTCATTTAATGATGTATG+TGG + chr1_1:77341663-77341682 None:intergenic 25.0%
!! TTCAATTCTCTTTCTTTTCT+TGG - chr1_1:77341871-77341890 Msa0045410:intron 25.0%
!! CAATTTTCATTGATACATCA+AGG - chr1_1:77341925-77341944 Msa0045410:intron 25.0%
! TACAACCGTTAATAATATTG+AGG + chr1_1:77342572-77342591 None:intergenic 25.0%
! ATATTATTAACGGTTGTATC+TGG - chr1_1:77342574-77342593 Msa0045410:intron 25.0%
! CCAAAAGTAAAAAATGGTAT+AGG + chr1_1:77342983-77343002 None:intergenic 25.0%
!!! CCTATACCATTTTTTACTTT+TGG - chr1_1:77342980-77342999 Msa0045410:intron 25.0%
!!! TACCATTTTTTACTTTTGGT+TGG - chr1_1:77342984-77343003 Msa0045410:intron 25.0%
!!! ACCATTTTTTACTTTTGGTT+GGG - chr1_1:77342985-77343004 Msa0045410:intron 25.0%
TATAAGCAATATCACAAGTG+AGG + chr1_1:77339946-77339965 None:intergenic 30.0%
TCTTATTGCCTTAGATTAAG+CGG - chr1_1:77339981-77340000 Msa0045410:three_prime_UTR 30.0%
AAAATAGACCGCTTAATCTA+AGG + chr1_1:77339992-77340011 None:intergenic 30.0%
ACAAATCTTGATTTATGCAG+GGG - chr1_1:77340080-77340099 Msa0045410:three_prime_UTR 30.0%
ACCAAAATTACCAGAATAAG+GGG + chr1_1:77340272-77340291 None:intergenic 30.0%
!! TTTTTCCTTTCCAGTATTGA+TGG - chr1_1:77340306-77340325 Msa0045410:three_prime_UTR 30.0%
!! TTCATTTTTCTTTGGGGATT+GGG - chr1_1:77340408-77340427 Msa0045410:three_prime_UTR 30.0%
!!! GGATCAACATATTGTTTTTG+AGG - chr1_1:77340662-77340681 Msa0045410:intron 30.0%
! ATGTCTGTACAATTGCTATT+GGG - chr1_1:77340785-77340804 Msa0045410:CDS 30.0%
AGAATGGTAAACACAGTAAA+AGG + chr1_1:77341024-77341043 None:intergenic 30.0%
! TCTCCTGTATTTTTCTTTAG+AGG - chr1_1:77341041-77341060 Msa0045410:intron 30.0%
AGATCTGTATACATATAACC+AGG + chr1_1:77341228-77341247 None:intergenic 30.0%
CATGGTTAGAATTGGAATTT+GGG + chr1_1:77341304-77341323 None:intergenic 30.0%
!! TGTCTTCTGTTTTGGATAAA+AGG - chr1_1:77341353-77341372 Msa0045410:intron 30.0%
GTAATATATCTGGTAAGATC+AGG + chr1_1:77341448-77341467 None:intergenic 30.0%
TGCATTATTACGAGAGAATT+TGG - chr1_1:77341571-77341590 Msa0045410:CDS 30.0%
!!! TGCTTATATTTTGGAACAGA+GGG + chr1_1:77341625-77341644 None:intergenic 30.0%
!!! TTGCTTATATTTTGGAACAG+AGG + chr1_1:77341626-77341645 None:intergenic 30.0%
GATGTATGTGGTTCATAATA+TGG + chr1_1:77341690-77341709 None:intergenic 30.0%
!!! TTGCTTATATTTTGAAACGG+AGG - chr1_1:77341739-77341758 Msa0045410:CDS 30.0%
!!! TGCTTATATTTTGAAACGGA+GGG - chr1_1:77341740-77341759 Msa0045410:CDS 30.0%
! TTGTGCTATATAATGGGATT+CGG - chr1_1:77341812-77341831 Msa0045410:CDS 30.0%
!! GGTATTCTCTCTGAAAATTT+TGG - chr1_1:77341833-77341852 Msa0045410:CDS 30.0%
CAGGGATCATATATATTTCA+TGG + chr1_1:77341958-77341977 None:intergenic 30.0%
CAATCTCATACAATTCATCA+GGG + chr1_1:77341976-77341995 None:intergenic 30.0%
TAATGCTTAGAATGTTTACC+AGG + chr1_1:77342020-77342039 None:intergenic 30.0%
! TTTCTTAATCAAAAGGTGTG+TGG - chr1_1:77342167-77342186 Msa0045410:CDS 30.0%
ACACTCTAATGATGTATCAT+TGG - chr1_1:77342193-77342212 Msa0045410:CDS 30.0%
!! TATGATGGATAGAACAAATG+CGG + chr1_1:77342871-77342890 None:intergenic 30.0%
TATAGGAATCACAACATCTA+CGG + chr1_1:77342966-77342985 None:intergenic 30.0%
TAATAAGGATAGTTGTCACT+TGG - chr1_1:77343130-77343149 Msa0045410:intron 30.0%
ACGAACAGATAAATACATCA+AGG + chr1_1:77343162-77343181 None:intergenic 30.0%
!!! TGAAACATGCTTTTTTAGTC+TGG - chr1_1:77343203-77343222 Msa0045410:CDS 30.0%
!!! GAAACATGCTTTTTTAGTCT+GGG - chr1_1:77343204-77343223 Msa0045410:CDS 30.0%
!!! AAACATGCTTTTTTAGTCTG+GGG - chr1_1:77343205-77343224 Msa0045410:CDS 30.0%
AATACGTCAGTAGGAACAAA+GGG + chr1_1:77339857-77339876 None:intergenic 35.0%
TAATACGTCAGTAGGAACAA+AGG + chr1_1:77339858-77339877 None:intergenic 35.0%
ATTCATGTCAGTTTCTTCAC+AGG + chr1_1:77339911-77339930 None:intergenic 35.0%
CAAATCTTGATTTATGCAGG+GGG - chr1_1:77340081-77340100 Msa0045410:three_prime_UTR 35.0%
!!! GTTTTTTAAGCGTGACTTTG+AGG - chr1_1:77340167-77340186 Msa0045410:three_prime_UTR 35.0%
! AGTGACCCTTTTCCTTAATT+TGG - chr1_1:77340209-77340228 Msa0045410:three_prime_UTR 35.0%
TCTTTAATTCCCCCTTATTC+TGG - chr1_1:77340259-77340278 Msa0045410:three_prime_UTR 35.0%
CCAAAATTACCAGAATAAGG+GGG + chr1_1:77340271-77340290 None:intergenic 35.0%
TTCAACCATCAATACTGGAA+AGG + chr1_1:77340314-77340333 None:intergenic 35.0%
!! TTCCAGTATTGATGGTTGAA+TGG - chr1_1:77340314-77340333 Msa0045410:three_prime_UTR 35.0%
!! CTTCATTTTTCTTTGGGGAT+TGG - chr1_1:77340407-77340426 Msa0045410:three_prime_UTR 35.0%
!! TCATTTTTCTTTGGGGATTG+GGG - chr1_1:77340409-77340428 Msa0045410:three_prime_UTR 35.0%
!! ACTTCACATGTGTTTGGTTT+TGG + chr1_1:77340554-77340573 None:intergenic 35.0%
TGTACAGACATGTAAGAAAC+AGG + chr1_1:77340776-77340795 None:intergenic 35.0%
CATGTCTGTACAATTGCTAT+TGG - chr1_1:77340784-77340803 Msa0045410:CDS 35.0%
! TTGCTATTGGGATTTACTGT+AGG - chr1_1:77340797-77340816 Msa0045410:intron 35.0%
TCTTACTACTGCTGAATATC+TGG - chr1_1:77340863-77340882 Msa0045410:intron 35.0%
! GAATATCTGGCTTATGAATG+TGG - chr1_1:77340876-77340895 Msa0045410:intron 35.0%
GCAACAATTTATGAACATGC+CGG - chr1_1:77341249-77341268 Msa0045410:intron 35.0%
GCATGGTTAGAATTGGAATT+TGG + chr1_1:77341305-77341324 None:intergenic 35.0%
TCATTTGGCATGGTTAGAAT+TGG + chr1_1:77341312-77341331 None:intergenic 35.0%
ATTCTAACCATGCCAAATGA+TGG - chr1_1:77341312-77341331 Msa0045410:intron 35.0%
TTCATTACCATCATTTGGCA+TGG + chr1_1:77341322-77341341 None:intergenic 35.0%
TCTTACCAGATATATTACGG+AGG - chr1_1:77341450-77341469 Msa0045410:intron 35.0%
CTTACCAGATATATTACGGA+GGG - chr1_1:77341451-77341470 Msa0045410:intron 35.0%
AACTAATCCAACATGGCTTA+TGG + chr1_1:77341549-77341568 None:intergenic 35.0%
ATGCAGTAACTAATCCAACA+TGG + chr1_1:77341556-77341575 None:intergenic 35.0%
!! TGTTGGCTTGTGCTATATAA+TGG - chr1_1:77341805-77341824 Msa0045410:CDS 35.0%
!! GTTGGCTTGTGCTATATAAT+GGG - chr1_1:77341806-77341825 Msa0045410:CDS 35.0%
CCAATCTCATACAATTCATC+AGG + chr1_1:77341977-77341996 None:intergenic 35.0%
CCTGATGAATTGTATGAGAT+TGG - chr1_1:77341974-77341993 Msa0045410:intron 35.0%
! ATGGAAGAACATTGATTGGT+GGG + chr1_1:77342076-77342095 None:intergenic 35.0%
! AGTGATGGAAGAACATTGAT+TGG + chr1_1:77342080-77342099 None:intergenic 35.0%
ATGTTCTTCCATCACTATCT+CGG - chr1_1:77342084-77342103 Msa0045410:CDS 35.0%
GATTTCAAAGTTCTGTCAAG+TGG - chr1_1:77342134-77342153 Msa0045410:CDS 35.0%
AGAGAAAATTAGTAGATGCC+AGG + chr1_1:77342481-77342500 None:intergenic 35.0%
TAGTGAAATTGTCCTTGAAG+TGG - chr1_1:77342526-77342545 Msa0045410:intron 35.0%
AGTGAAATTGTCCTTGAAGT+GGG - chr1_1:77342527-77342546 Msa0045410:intron 35.0%
CTTCATTAATACCCACTTCA+AGG + chr1_1:77342541-77342560 None:intergenic 35.0%
! AATTTCTGGACAAGTTTGAG+AGG - chr1_1:77342597-77342616 Msa0045410:intron 35.0%
TAGAGACTGGAATATATTGC+AGG + chr1_1:77342651-77342670 None:intergenic 35.0%
ATCATCAATTACCAGCATCT+CGG + chr1_1:77342757-77342776 None:intergenic 35.0%
AATCAATTGCCCATGACATT+AGG + chr1_1:77342823-77342842 None:intergenic 35.0%
ATAGGAATCACAACATCTAC+GGG + chr1_1:77342965-77342984 None:intergenic 35.0%
GCCCAACCAAAAGTAAAAAA+TGG + chr1_1:77342989-77343008 None:intergenic 35.0%
GAAACAAGCAAACATACACT+GGG + chr1_1:77343068-77343087 None:intergenic 35.0%
TGAAACAAGCAAACATACAC+TGG + chr1_1:77343069-77343088 None:intergenic 35.0%
AGTTTCTTCACAGGAGTTGT+CGG + chr1_1:77339902-77339921 None:intergenic 40.0%
!!! TGCCATTGTTTTTGCAGCTA+TGG - chr1_1:77340122-77340141 Msa0045410:three_prime_UTR 40.0%
!!! GCCATTGTTTTTGCAGCTAT+GGG - chr1_1:77340123-77340142 Msa0045410:three_prime_UTR 40.0%
TCCCATAGCTGCAAAAACAA+TGG + chr1_1:77340127-77340146 None:intergenic 40.0%
! CCCCCTTATTCTGGTAATTT+TGG - chr1_1:77340268-77340287 Msa0045410:three_prime_UTR 40.0%
AGCCATTCAACCATCAATAC+TGG + chr1_1:77340319-77340338 None:intergenic 40.0%
ACCAAACACATGTGAAGTGT+AGG - chr1_1:77340556-77340575 Msa0045410:CDS 40.0%
CCAAACACATGTGAAGTGTA+GGG - chr1_1:77340557-77340576 Msa0045410:CDS 40.0%
GTGATGATACGTTCGATTGT+AGG + chr1_1:77340831-77340850 None:intergenic 40.0%
TCACTTGTTCTTGATCCTAC+AGG - chr1_1:77341161-77341180 Msa0045410:intron 40.0%
TATCTGGTAAGATCAGGAGT+TGG + chr1_1:77341442-77341461 None:intergenic 40.0%
AGGGACAGATATACATCGAT+AGG - chr1_1:77341470-77341489 Msa0045410:intron 40.0%
TAGGCAGTTGCATAATAGAC+AGG - chr1_1:77341489-77341508 Msa0045410:intron 40.0%
CAGTTGCATAATAGACAGGT+GGG - chr1_1:77341493-77341512 Msa0045410:intron 40.0%
ATGAATGCCATAAGCCATGT+TGG - chr1_1:77341539-77341558 Msa0045410:CDS 40.0%
TACAATCAGTCTAAGTGACC+TGG - chr1_1:77341999-77342018 Msa0045410:intron 40.0%
! GATGGAAGAACATTGATTGG+TGG + chr1_1:77342077-77342096 None:intergenic 40.0%
TTCATCAACCGAGATAGTGA+TGG + chr1_1:77342095-77342114 None:intergenic 40.0%
!! ATCACTATCTCGGTTGATGA+AGG - chr1_1:77342094-77342113 Msa0045410:CDS 40.0%
CTGTATGCAACTTATGCCAT+TGG - chr1_1:77342398-77342417 Msa0045410:intron 40.0%
TGCAACTTATGCCATTGGAA+AGG - chr1_1:77342403-77342422 Msa0045410:intron 40.0%
!! AGAAGAAGCACTTTCATCTG+AGG - chr1_1:77342454-77342473 Msa0045410:intron 40.0%
! ACGGTTGTATCTGGAATTTC+TGG - chr1_1:77342583-77342602 Msa0045410:intron 40.0%
! GAATATATTGCAGGTGTCGT+AGG + chr1_1:77342642-77342661 None:intergenic 40.0%
ATCTTGCATGGACTTTACTC+CGG - chr1_1:77342672-77342691 Msa0045410:intron 40.0%
! TACGATGAAGAAGTACACGA+GGG + chr1_1:77342703-77342722 None:intergenic 40.0%
! ATACGATGAAGAAGTACACG+AGG + chr1_1:77342704-77342723 None:intergenic 40.0%
GTAAAACTGGTCAAGAGTCT+TGG + chr1_1:77342732-77342751 None:intergenic 40.0%
GATAGAACAAATGCGGACTT+AGG + chr1_1:77342864-77342883 None:intergenic 40.0%
CTGAACAGGCTCAAAATATG+AGG + chr1_1:77342925-77342944 None:intergenic 40.0%
TTCTTTCGGACTAACTGAAC+AGG + chr1_1:77342939-77342958 None:intergenic 40.0%
ACATCTACGGGTTCTTCTTT+CGG + chr1_1:77342953-77342972 None:intergenic 40.0%
TACTTGTGTTGCACACAGTT+TGG - chr1_1:77343016-77343035 Msa0045410:intron 40.0%
ACTTGTGTTGCACACAGTTT+GGG - chr1_1:77343017-77343036 Msa0045410:intron 40.0%
! TTTGGGATGGTTGGAATTTG+TGG - chr1_1:77343034-77343053 Msa0045410:intron 40.0%
!!! CTTTTTTAGTCTGGGGAATC+TGG - chr1_1:77343212-77343231 Msa0045410:CDS 40.0%
ATCTTGATTTATGCAGGGGG+AGG - chr1_1:77340084-77340103 Msa0045410:three_prime_UTR 45.0%
TGCAGCTATGGGAGTTAACA+TGG - chr1_1:77340134-77340153 Msa0045410:three_prime_UTR 45.0%
! AAGCGTGACTTTGAGGAGAA+TGG - chr1_1:77340174-77340193 Msa0045410:three_prime_UTR 45.0%
! CTTTGAGGAGAATGGCTCAA+TGG - chr1_1:77340182-77340201 Msa0045410:three_prime_UTR 45.0%
CCCTACACTTCACATGTGTT+TGG + chr1_1:77340560-77340579 None:intergenic 45.0%
GCAGTAGTAAGAGCAATACG+AGG + chr1_1:77340855-77340874 None:intergenic 45.0%
TTATGCTGATGCTCTTCGTG+AGG - chr1_1:77340935-77340954 Msa0045410:CDS 45.0%
TGAACATGCCGGAAGAATTG+AGG - chr1_1:77341260-77341279 Msa0045410:intron 45.0%
GAACATGCCGGAAGAATTGA+GGG - chr1_1:77341261-77341280 Msa0045410:intron 45.0%
AACATGCCGGAAGAATTGAG+GGG - chr1_1:77341262-77341281 Msa0045410:intron 45.0%
TTTACGCCCCTCAATTCTTC+CGG + chr1_1:77341271-77341290 None:intergenic 45.0%
CTGTCCCTCCGTAATATATC+TGG + chr1_1:77341458-77341477 None:intergenic 45.0%
GCAGTTGCATAATAGACAGG+TGG - chr1_1:77341492-77341511 Msa0045410:intron 45.0%
TGGCTTATGGCATTCATCAC+AGG + chr1_1:77341536-77341555 None:intergenic 45.0%
TCATTCTGATGTGTCCAACC+AGG - chr1_1:77342260-77342279 Msa0045410:intron 45.0%
CAAACAAGAAGAGACCTGGT+TGG + chr1_1:77342277-77342296 None:intergenic 45.0%
GGAACAAACAAGAAGAGACC+TGG + chr1_1:77342281-77342300 None:intergenic 45.0%
TCCAGTCTCTAGATCTTGCA+TGG - chr1_1:77342660-77342679 Msa0045410:intron 45.0%
TCCATGCAAGATCTAGAGAC+TGG + chr1_1:77342664-77342683 None:intergenic 45.0%
GAAGTACACGAGGGAAAATC+CGG + chr1_1:77342694-77342713 None:intergenic 45.0%
GCTGATTGAGCAAGAGACTA+AGG + chr1_1:77342799-77342818 None:intergenic 45.0%
TCAATCAGCGCCTAATGTCA+TGG - chr1_1:77342810-77342829 Msa0045410:CDS 45.0%
CAATCAGCGCCTAATGTCAT+GGG - chr1_1:77342811-77342830 Msa0045410:CDS 45.0%
! GGAAGAATTGAGGGGCGTAA+AGG - chr1_1:77341270-77341289 Msa0045410:intron 50.0%
! TCACCACTGCAGAGTGCTAT+TGG - chr1_1:77342216-77342235 Msa0045410:intron 50.0%
! CACCACTGCAGAGTGCTATT+GGG - chr1_1:77342217-77342236 Msa0045410:intron 50.0%
! ACCACTGCAGAGTGCTATTG+GGG - chr1_1:77342218-77342237 Msa0045410:intron 50.0%
TGGGGAGGGAATGACTAGAA+AGG - chr1_1:77342236-77342255 Msa0045410:intron 50.0%
TGCCATTGGAAAGGATGTCC+AGG - chr1_1:77342412-77342431 Msa0045410:intron 50.0%
TGCCTGGACATCCTTTCCAA+TGG + chr1_1:77342417-77342436 None:intergenic 50.0%
CAACGACAGCTTTCATTGCC+TGG + chr1_1:77342433-77342452 None:intergenic 50.0%
CAGGCAATGAAAGCTGTCGT+TGG - chr1_1:77342431-77342450 Msa0045410:intron 50.0%
AGCACTTTCATCTGAGGACC+TGG - chr1_1:77342460-77342479 Msa0045410:intron 50.0%
!! TGAGAGGAAGTTTGTTGCCC+AGG - chr1_1:77342613-77342632 Msa0045410:intron 50.0%
!! GAGAGGAAGTTTGTTGCCCA+GGG - chr1_1:77342614-77342633 Msa0045410:intron 50.0%
AACTGGTCAAGAGTCTTGGC+TGG + chr1_1:77342728-77342747 None:intergenic 50.0%
CAGCATCTCGGCTGTAAAAC+TGG + chr1_1:77342745-77342764 None:intergenic 50.0%
! CAGTTTTACAGCCGAGATGC+TGG - chr1_1:77342743-77342762 Msa0045410:intron 50.0%
GTGTTGCACACAGTTTGGGA+TGG - chr1_1:77343021-77343040 Msa0045410:intron 50.0%
! TGCACACAGTTTGGGATGGT+TGG - chr1_1:77343025-77343044 Msa0045410:intron 50.0%
CTCTGGCAGCAGATACCTGT+AGG + chr1_1:77341179-77341198 None:intergenic 55.0%
GGTATCTGCTGCCAGAGAAG+AGG - chr1_1:77341182-77341201 Msa0045410:intron 55.0%
TCTTCCTGGCACCTCTTCTC+TGG + chr1_1:77341196-77341215 None:intergenic 55.0%
CCAGGATAACCACGTCTTCC+TGG + chr1_1:77341210-77341229 None:intergenic 55.0%
CCAGGAAGACGTGGTTATCC+TGG - chr1_1:77341207-77341226 Msa0045410:intron 55.0%
TCCCCAATAGCACTCTGCAG+TGG + chr1_1:77342222-77342241 None:intergenic 55.0%
! ACTGCAGAGTGCTATTGGGG+AGG - chr1_1:77342221-77342240 Msa0045410:intron 55.0%
! CTGCAGAGTGCTATTGGGGA+GGG - chr1_1:77342222-77342241 Msa0045410:intron 55.0%
!! GAAGAGGTGCCAGGAAGACG+TGG - chr1_1:77341198-77341217 Msa0045410:intron 60.0%
!! GCTGCCAGAGAAGAGGTGCC+AGG - chr1_1:77341189-77341208 Msa0045410:intron 65.0%
! GCAGGTGTCGTAGGCTCCCT+GGG + chr1_1:77342633-77342652 None:intergenic 65.0%
! TGCAGGTGTCGTAGGCTCCC+TGG + chr1_1:77342634-77342653 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 77339800 77343236 77339800 ID=Msa0045410;Name=Msa0045410
chr1_1 mRNA 77339800 77343236 77339800 ID=Msa0045410-mRNA-1;Parent=Msa0045410;Name=Msa0045410-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|0.85|0.87|0.87|0.85|0.87|8|653|274
chr1_1 exon 77339800 77340638 77339800 ID=Msa0045410-mRNA-1:exon:26410;Parent=Msa0045410-mRNA-1
chr1_1 exon 77340755 77340808 77340755 ID=Msa0045410-mRNA-1:exon:26409;Parent=Msa0045410-mRNA-1
chr1_1 exon 77340921 77340968 77340921 ID=Msa0045410-mRNA-1:exon:26408;Parent=Msa0045410-mRNA-1
chr1_1 exon 77341526 77341608 77341526 ID=Msa0045410-mRNA-1:exon:26407;Parent=Msa0045410-mRNA-1
chr1_1 exon 77341703 77341853 77341703 ID=Msa0045410-mRNA-1:exon:26406;Parent=Msa0045410-mRNA-1
chr1_1 exon 77342080 77342217 77342080 ID=Msa0045410-mRNA-1:exon:26405;Parent=Msa0045410-mRNA-1
chr1_1 exon 77342806 77342936 77342806 ID=Msa0045410-mRNA-1:exon:26404;Parent=Msa0045410-mRNA-1
chr1_1 exon 77343203 77343236 77343203 ID=Msa0045410-mRNA-1:exon:26403;Parent=Msa0045410-mRNA-1
chr1_1 CDS 77343203 77343236 77343203 ID=Msa0045410-mRNA-1:cds;Parent=Msa0045410-mRNA-1
chr1_1 CDS 77342806 77342936 77342806 ID=Msa0045410-mRNA-1:cds;Parent=Msa0045410-mRNA-1
chr1_1 CDS 77342080 77342217 77342080 ID=Msa0045410-mRNA-1:cds;Parent=Msa0045410-mRNA-1
chr1_1 CDS 77341703 77341853 77341703 ID=Msa0045410-mRNA-1:cds;Parent=Msa0045410-mRNA-1
chr1_1 CDS 77341526 77341608 77341526 ID=Msa0045410-mRNA-1:cds;Parent=Msa0045410-mRNA-1
chr1_1 CDS 77340921 77340968 77340921 ID=Msa0045410-mRNA-1:cds;Parent=Msa0045410-mRNA-1
chr1_1 CDS 77340755 77340808 77340755 ID=Msa0045410-mRNA-1:cds;Parent=Msa0045410-mRNA-1
chr1_1 CDS 77340453 77340638 77340453 ID=Msa0045410-mRNA-1:cds;Parent=Msa0045410-mRNA-1
chr1_1 three_prime_UTR 77339800 77340452 77339800 ID=Msa0045410-mRNA-1:three_prime_utr;Parent=Msa0045410-mRNA-1
Gene Sequence

>Msa0045410

ATGCTATGTGATGATAACATAAAGCAAACTCAAAGGGGAGGAGAAGACGACAATTTTGCCATTGTTTTTGCAGCTATGGGAGTTAACATGGAGACTGCACAGTTTTTTAAGCGTGACTTTGAGGAGAATGGCTCAATGGAGAGAGTGACCCTTTTCCTTAATTTGGCAAATGATCCTACAATCGAACGTATCATCACTCCTCGTATTGCTCTTACTACTGCTGAATATCTGGCTTATGAATGTGGCAAGCATGTTCTCGTGATACTCACAGATATGAGTTCTTATGCTGATGCTCTTCGTGAGGTATCTGCTGCCAGAGAAGAGGTGCCAGGAAGACGTGGTTATCCTGGTTATATGTATACAGATCTTGCAACAATTTATGAACATGCCGGAAGAATTGAGGGGCGTAAAGGCTCAATTACCCAAATTCCAATTCTAACCATGCCAAATGATGATATTACACATCCAACTCCTGATCTTACCAGATATATTACGGAGGGACAGATATACATCGATAGGCAGTTGCATAATAGACAGATATACCCACCAATCAATGTTCTTCCATCACTATCTCGGTTGATGAAGAGTGCTATTGGGGAGGGAATGACTAGAAAGGATCATTCTGATGTGTCCAACCAGCTGTATGCAACTTATGCCATTGGAAAGGATGTCCAGGCAATGAAAGCTGTCGTTGGAGAAGAAGCACTTTCATCTGAGGACCTGGCATCTACTAATTTTCTCTCGAGCTGTTTTTTTTTTTTTTTATATAGTGAAATTGTCCTTGAAGTGGGTATTAATGAAGCTTATTTTCCTCAATATTATTAA
Protein sequence

>Msa0045410

MLCDDNIKQTQRGGEDDNFAIVFAAMGVNMETAQFFKRDFEENGSMERVTLFLNLANDPTIERIITPRIALTTAEYLAYECGKHVLVILTDMSSYADALREVSAAREEVPGRRGYPGYMYTDLATIYEHAGRIEGRKGSITQIPILTMPNDDITHPTPDLTRYITEGQIYIDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHSDVSNQLYATYAIGKDVQAMKAVVGEEALSSEDLASTNFLSSCFFFFLYSEIVLEVGINEAYFPQYY*