AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0050900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0050900 MtrunA17_Chr1g0168341 100.000 239 0 0 1 239 1 239 2.88e-177 486
Msa0050900 MtrunA17_Chr1g0155631 99.582 239 1 0 1 239 1 239 4.92e-177 485
Msa0050900 MtrunA17_Chr6g0469891 94.444 234 8 1 5 238 1 229 1.04e-162 449
Msa0050900 MtrunA17_Chr6g0469871 94.444 234 8 1 5 238 1 229 1.04e-162 449
Msa0050900 MtrunA17_Chr1g0210251 86.383 235 31 1 1 234 1 235 4.02e-150 417
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0050900 AT5G35530.1 88.841 233 26 0 1 233 1 233 2.12e-153 426
Msa0050900 AT2G31610.1 88.987 227 25 0 1 227 1 227 4.58e-149 416
Msa0050900 AT3G53870.1 92.166 217 17 0 1 217 1 217 1.53e-148 414

Find 88 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGGGTGTTCTTGGTATTA+AGG 0.259759 1_2:-4923642 Msa0050900:intron
TTAGGACCTTGCTTTCCTTT+AGG 0.279269 1_2:+4923596 None:intergenic
AGGCATGTTCTAAGAGTATA+TGG 0.307014 1_2:-4923211 Msa0050900:three_prime_UTR
TAATACACGGGAGGAAATTA+TGG 0.314729 1_2:+4923281 None:intergenic
GCTATTCCGGTGTTGAGGTT+AGG 0.315077 1_2:-4924255 Msa0050900:CDS
CTTGCTGAAGATGGCTATTC+CGG 0.326131 1_2:-4924268 Msa0050900:CDS
TCCCCTGTTGTTAACCTTCT+CGG 0.326817 1_2:+4924005 None:intergenic
CGGTGTGTTGAGGTTTGTTA+TGG 0.332791 1_2:-4923908 Msa0050900:CDS
ACTCGTACCCAAGCTGTTCT+TGG 0.341791 1_2:-4924196 Msa0050900:intron
CATGTTTGCAGATTTAGCTT+GGG 0.343700 1_2:+4924473 None:intergenic
GATGGATGGATGTTTATATA+AGG 0.347163 1_2:+4924575 None:intergenic
GATGTACTCTTCCTCCTCCT+TGG 0.351127 1_2:+4923538 None:intergenic
AAACCTCAACACACCGTAAC+AGG 0.355716 1_2:+4923915 None:intergenic
CTCATCTGGCCAACCTGTTA+AGG 0.357556 1_2:-4923794 Msa0050900:CDS
ATAGCCATCCTTGAACTTCA+TGG 0.360301 1_2:+4923822 None:intergenic
GAAACTGCAAACCCTAGTTT+TGG 0.367479 1_2:+4924539 None:intergenic
CTATTCCGGTGTTGAGGTTA+GGG 0.371348 1_2:-4924254 Msa0050900:CDS
CCATGTTTGCAGATTTAGCT+TGG 0.385717 1_2:+4924472 None:intergenic
GGATGTGAGGTTATTGTTAG+TGG 0.386156 1_2:-4923871 Msa0050900:CDS
CTAACCATTTCTCCAAAACT+AGG 0.397229 1_2:-4924551 Msa0050900:five_prime_UTR
GAGTCAATGTAATCCTTAAC+AGG 0.402767 1_2:+4923781 None:intergenic
ATCCTTGAACTTCATGGACT+TGG 0.424680 1_2:+4923828 None:intergenic
ATGTACTCTTCCTCCTCCTT+GGG 0.427577 1_2:+4923539 None:intergenic
GTGTGAATGGTAACAATATC+AGG 0.428011 1_2:+4923563 None:intergenic
AGGTGAAGATTATGCTTGAC+TGG 0.432560 1_2:-4923622 Msa0050900:CDS
CTCCTCCTTGGGAGTGTGAA+TGG 0.434959 1_2:+4923550 None:intergenic
ACCAGAGAGCTTGCTGAAGA+TGG 0.438488 1_2:-4924277 Msa0050900:CDS
AGCTGAGTATAACATTATCT+TGG 0.450468 1_2:+4923340 None:intergenic
GATGGCTATATGATCTCATC+TGG 0.452022 1_2:-4923808 Msa0050900:CDS
TCTCTTCGTTACAAGCTTCT+CGG 0.453319 1_2:-4923958 Msa0050900:CDS
ATGCCGAGAAGGTTAACAAC+AGG 0.456910 1_2:-4924008 Msa0050900:CDS
CAAACAACAGGGACTGGGAC+AGG 0.457728 1_2:+4923479 None:intergenic
AGGAGGAGGAAGAGTACATC+AGG 0.462947 1_2:-4923535 Msa0050900:CDS
GAGAAATGGTTAGGGATGGA+TGG 0.468403 1_2:+4924561 None:intergenic
TTGAGGTTTGTTATGGAAAG+TGG 0.475116 1_2:-4923901 Msa0050900:CDS
ATGTTTGCAGATTTAGCTTG+GGG 0.476401 1_2:+4924474 None:intergenic
AATGATTAGGTGAGAAGGGA+AGG 0.478164 1_2:-4924098 Msa0050900:intron
TTTGGAGAAATGGTTAGGGA+TGG 0.483044 1_2:+4924557 None:intergenic
GATTAGGTGAGAAGGGAAGG+AGG 0.484267 1_2:-4924095 Msa0050900:intron
TATAGTTCAACACTGTTCTC+AGG 0.488325 1_2:+4924030 None:intergenic
GCCATCTTCAGCAAGCTCTC+TGG 0.491889 1_2:+4924276 None:intergenic
AGCCAAGTCCATGAAGTTCA+AGG 0.492737 1_2:-4923830 Msa0050900:CDS
TGTTATGGAAAGTGGTGCTA+AGG 0.499768 1_2:-4923893 Msa0050900:CDS
TAGACATGTTCTTCTCAGAC+AGG 0.500852 1_2:-4923752 Msa0050900:intron
TTGTTGCAGTTCGTCGCTGA+CGG 0.501712 1_2:-4924331 Msa0050900:intron
TGACCTCAGTAGTCCAGAAA+AGG 0.517945 1_2:-4924062 Msa0050900:CDS
GTTTGCAGATTTAGCTTGGG+GGG 0.518937 1_2:+4924476 None:intergenic
CTACGAACCAAGAACAGCTT+GGG 0.525454 1_2:+4924189 None:intergenic
GATGATGATCTCAGTGCGCA+TGG 0.527229 1_2:+4924225 None:intergenic
TCATTTGCAACAACAGCAGC+AGG 0.528096 1_2:+4923512 None:intergenic
GATTAATGATTAGGTGAGAA+GGG 0.529224 1_2:-4924102 Msa0050900:intron
CCAAGCTAAATCTGCAAACA+TGG 0.529777 1_2:-4924472 Msa0050900:exon
TAACCATTTCTCCAAAACTA+GGG 0.529900 1_2:-4924550 Msa0050900:five_prime_UTR
TTTCTTCGCTGAATTGAATG+AGG 0.530015 1_2:-4924305 Msa0050900:CDS
AATGGTAACAATATCAGGTA+GGG 0.532486 1_2:+4923568 None:intergenic
TAAGATGTAAATATTAGATA+AGG 0.537093 1_2:+4923254 None:intergenic
GCTGTTCGCAGAGCCTGTTA+CGG 0.553152 1_2:-4923928 Msa0050900:CDS
GAGCCTGTTACGGTGTGTTG+AGG 0.556363 1_2:-4923918 Msa0050900:CDS
TGATTAATGATTAGGTGAGA+AGG 0.556668 1_2:-4924103 Msa0050900:intron
ATGCTTGACTGGGATCCTAA+AGG 0.556783 1_2:-4923611 Msa0050900:CDS
TGTTGTTGCAAATGATATTG+AGG 0.562213 1_2:-4923504 Msa0050900:CDS
ACTACGAACCAAGAACAGCT+TGG 0.565206 1_2:+4924188 None:intergenic
TTTCTCAAACAACAGGGACT+GGG 0.568525 1_2:+4923474 None:intergenic
GTTATGGAAAGTGGTGCTAA+GGG 0.582081 1_2:-4923892 Msa0050900:CDS
GAATGGTAACAATATCAGGT+AGG 0.585408 1_2:+4923567 None:intergenic
AGATGGCTATTCCGGTGTTG+AGG 0.587442 1_2:-4924260 Msa0050900:CDS
GGATGGATGTTTATATAAGG+TGG 0.588162 1_2:+4924578 None:intergenic
CATTCACACTCCCAAGGAGG+AGG 0.589806 1_2:-4923549 Msa0050900:CDS
CAATGTAATCCTTAACAGGT+TGG 0.596885 1_2:+4923785 None:intergenic
TGGGATCCTAAAGGAAAGCA+AGG 0.601952 1_2:-4923602 Msa0050900:CDS
GGTGAAGATTATGCTTGACT+GGG 0.603451 1_2:-4923621 Msa0050900:CDS
TTTGCAGATTTAGCTTGGGG+GGG 0.606024 1_2:+4924477 None:intergenic
AAACAACAGGGACTGGGACA+GGG 0.607283 1_2:+4923480 None:intergenic
TGTTTGCAGATTTAGCTTGG+GGG 0.615419 1_2:+4924475 None:intergenic
TGTTGAACTATATGCCGAGA+AGG 0.615665 1_2:-4924019 Msa0050900:CDS
ATGGTAACAATATCAGGTAG+GGG 0.623679 1_2:+4923569 None:intergenic
TGTTACCATTCACACTCCCA+AGG 0.629341 1_2:-4923555 Msa0050900:CDS
AAGTCCATGAAGTTCAAGGA+TGG 0.640546 1_2:-4923826 Msa0050900:CDS
ATGATGATCTCAGTGCGCAT+GGG 0.667262 1_2:+4924226 None:intergenic
AAGTGGTGCTAAGGGATGTG+AGG 0.670962 1_2:-4923884 Msa0050900:CDS
CTTCGTTACAAGCTTCTCGG+AGG 0.671532 1_2:-4923955 Msa0050900:CDS
TGCCGAGAAGGTTAACAACA+GGG 0.674234 1_2:-4924007 Msa0050900:CDS
AGATAAGGAACATAATACAC+GGG 0.677461 1_2:+4923269 None:intergenic
TAAGGAACATAATACACGGG+AGG 0.680426 1_2:+4923272 None:intergenic
AGTAACCCTAACCTCAACAC+CGG 0.681090 1_2:+4924249 None:intergenic
GCCGAGAAGGTTAACAACAG+GGG 0.682645 1_2:-4924006 Msa0050900:CDS
TAGATAAGGAACATAATACA+CGG 0.690016 1_2:+4923268 None:intergenic
TACCATTCACACTCCCAAGG+AGG 0.690748 1_2:-4923552 Msa0050900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTTTATATGAATTTAAA+TGG - chr1_2:4923593-4923612 Msa0050900:CDS 10.0%
!!! TGTTTTTATATGAATTTAAA+TGG - chr1_2:4923593-4923612 Msa0050900:CDS 10.0%
!!! ATTATTACCTTTATTTTGTA+GGG - chr1_2:4924067-4924086 Msa0050900:CDS 15.0%
!! TAAGATGTAAATATTAGATA+AGG + chr1_2:4924475-4924494 None:intergenic 15.0%
!!! ATTATTACCTTTATTTTGTA+GGG - chr1_2:4924067-4924086 Msa0050900:CDS 15.0%
!! TAAGATGTAAATATTAGATA+AGG + chr1_2:4924475-4924494 None:intergenic 15.0%
!!! GATTATTACCTTTATTTTGT+AGG - chr1_2:4924066-4924085 Msa0050900:CDS 20.0%
!!! TATATGGATTTTTGCATATT+TGG - chr1_2:4924531-4924550 Msa0050900:five_prime_UTR 20.0%
!!! ATATGGATTTTTGCATATTT+GGG - chr1_2:4924532-4924551 Msa0050900:five_prime_UTR 20.0%
!!! GATTATTACCTTTATTTTGT+AGG - chr1_2:4924066-4924085 Msa0050900:CDS 20.0%
!!! TATATGGATTTTTGCATATT+TGG - chr1_2:4924531-4924550 Msa0050900:five_prime_UTR 20.0%
!!! ATATGGATTTTTGCATATTT+GGG - chr1_2:4924532-4924551 Msa0050900:five_prime_UTR 20.0%
! GTTGAATCTGATTAATGATT+AGG - chr1_2:4923615-4923634 Msa0050900:CDS 25.0%
!! AAACATTTTCTCAAACAACA+GGG + chr1_2:4924261-4924280 None:intergenic 25.0%
!! AAAACATTTTCTCAAACAAC+AGG + chr1_2:4924262-4924281 None:intergenic 25.0%
! TAGATAAGGAACATAATACA+CGG + chr1_2:4924461-4924480 None:intergenic 25.0%
!!! GTTTTGTTGATGATATATTG+AGG - chr1_2:4924495-4924514 Msa0050900:five_prime_UTR 25.0%
! GTTGAATCTGATTAATGATT+AGG - chr1_2:4923615-4923634 Msa0050900:CDS 25.0%
!! AAACATTTTCTCAAACAACA+GGG + chr1_2:4924261-4924280 None:intergenic 25.0%
!! AAAACATTTTCTCAAACAAC+AGG + chr1_2:4924262-4924281 None:intergenic 25.0%
! TAGATAAGGAACATAATACA+CGG + chr1_2:4924461-4924480 None:intergenic 25.0%
!!! GTTTTGTTGATGATATATTG+AGG - chr1_2:4924495-4924514 Msa0050900:five_prime_UTR 25.0%
GATGGATGGATGTTTATATA+AGG + chr1_2:4923154-4923173 None:intergenic 30.0%
!!! TAGTTTTGGAGAAATGGTTA+GGG + chr1_2:4923176-4923195 None:intergenic 30.0%
TAACCATTTCTCCAAAACTA+GGG - chr1_2:4923176-4923195 Msa0050900:three_prime_UTR 30.0%
CACTACTAATAGAGATGAAT+TGG + chr1_2:4923334-4923353 None:intergenic 30.0%
TGATTAATGATTAGGTGAGA+AGG - chr1_2:4923623-4923642 Msa0050900:CDS 30.0%
GATTAATGATTAGGTGAGAA+GGG - chr1_2:4923624-4923643 Msa0050900:CDS 30.0%
AAACCAACAAGGTTGTTATA+GGG + chr1_2:4924012-4924031 None:intergenic 30.0%
AATAACAAACCAAACCAACA+AGG + chr1_2:4924023-4924042 None:intergenic 30.0%
AAGAACACCCTACAAAATAA+AGG + chr1_2:4924077-4924096 None:intergenic 30.0%
!!! TTTATTTTGTAGGGTGTTCT+TGG - chr1_2:4924076-4924095 Msa0050900:CDS 30.0%
AATGGTAACAATATCAGGTA+GGG + chr1_2:4924161-4924180 None:intergenic 30.0%
TGTTGTTGCAAATGATATTG+AGG - chr1_2:4924222-4924241 Msa0050900:CDS 30.0%
!! GAGAAAATGTTTTGAGTGTA+TGG - chr1_2:4924269-4924288 Msa0050900:CDS 30.0%
! TGTCAGACTTTTGTAGTTTT+CGG - chr1_2:4924322-4924341 Msa0050900:CDS 30.0%
AGCTGAGTATAACATTATCT+TGG + chr1_2:4924389-4924408 None:intergenic 30.0%
AGATAAGGAACATAATACAC+GGG + chr1_2:4924460-4924479 None:intergenic 30.0%
GATGGATGGATGTTTATATA+AGG + chr1_2:4923154-4923173 None:intergenic 30.0%
!!! TAGTTTTGGAGAAATGGTTA+GGG + chr1_2:4923176-4923195 None:intergenic 30.0%
TAACCATTTCTCCAAAACTA+GGG - chr1_2:4923176-4923195 Msa0050900:three_prime_UTR 30.0%
CACTACTAATAGAGATGAAT+TGG + chr1_2:4923334-4923353 None:intergenic 30.0%
TGATTAATGATTAGGTGAGA+AGG - chr1_2:4923623-4923642 Msa0050900:CDS 30.0%
GATTAATGATTAGGTGAGAA+GGG - chr1_2:4923624-4923643 Msa0050900:CDS 30.0%
AAACCAACAAGGTTGTTATA+GGG + chr1_2:4924012-4924031 None:intergenic 30.0%
AATAACAAACCAAACCAACA+AGG + chr1_2:4924023-4924042 None:intergenic 30.0%
AAGAACACCCTACAAAATAA+AGG + chr1_2:4924077-4924096 None:intergenic 30.0%
!!! TTTATTTTGTAGGGTGTTCT+TGG - chr1_2:4924076-4924095 Msa0050900:CDS 30.0%
AATGGTAACAATATCAGGTA+GGG + chr1_2:4924161-4924180 None:intergenic 30.0%
TGTTGTTGCAAATGATATTG+AGG - chr1_2:4924222-4924241 Msa0050900:CDS 30.0%
!! GAGAAAATGTTTTGAGTGTA+TGG - chr1_2:4924269-4924288 Msa0050900:CDS 30.0%
! TGTCAGACTTTTGTAGTTTT+CGG - chr1_2:4924322-4924341 Msa0050900:CDS 30.0%
AGCTGAGTATAACATTATCT+TGG + chr1_2:4924389-4924408 None:intergenic 30.0%
AGATAAGGAACATAATACAC+GGG + chr1_2:4924460-4924479 None:intergenic 30.0%
GGATGGATGTTTATATAAGG+TGG + chr1_2:4923151-4923170 None:intergenic 35.0%
!!! CTAGTTTTGGAGAAATGGTT+AGG + chr1_2:4923177-4923196 None:intergenic 35.0%
CTAACCATTTCTCCAAAACT+AGG - chr1_2:4923175-4923194 Msa0050900:three_prime_UTR 35.0%
!!! AAACCCTAGTTTTGGAGAAA+TGG + chr1_2:4923182-4923201 None:intergenic 35.0%
!! ATGTTTGCAGATTTAGCTTG+GGG + chr1_2:4923255-4923274 None:intergenic 35.0%
! CATGTTTGCAGATTTAGCTT+GGG + chr1_2:4923256-4923275 None:intergenic 35.0%
!!! CTTTCGCTTTTTGCTCATTT+GGG + chr1_2:4923283-4923302 None:intergenic 35.0%
TTTCTTCGCTGAATTGAATG+AGG - chr1_2:4923421-4923440 Msa0050900:three_prime_UTR 35.0%
! CAGGAAACTTAAACCTTTTC+TGG + chr1_2:4923680-4923699 None:intergenic 35.0%
TATAGTTCAACACTGTTCTC+AGG + chr1_2:4923699-4923718 None:intergenic 35.0%
!! TTGAGGTTTGTTATGGAAAG+TGG - chr1_2:4923825-4923844 Msa0050900:CDS 35.0%
CAATGTAATCCTTAACAGGT+TGG + chr1_2:4923944-4923963 None:intergenic 35.0%
GAGTCAATGTAATCCTTAAC+AGG + chr1_2:4923948-4923967 None:intergenic 35.0%
TTACCCTATAACAACCTTGT+TGG - chr1_2:4924006-4924025 Msa0050900:CDS 35.0%
CAAACCAACAAGGTTGTTAT+AGG + chr1_2:4924013-4924032 None:intergenic 35.0%
! CTATAACAACCTTGTTGGTT+TGG - chr1_2:4924011-4924030 Msa0050900:CDS 35.0%
ATGGTAACAATATCAGGTAG+GGG + chr1_2:4924160-4924179 None:intergenic 35.0%
GAATGGTAACAATATCAGGT+AGG + chr1_2:4924162-4924181 None:intergenic 35.0%
GTGTGAATGGTAACAATATC+AGG + chr1_2:4924166-4924185 None:intergenic 35.0%
TAATACACGGGAGGAAATTA+TGG + chr1_2:4924448-4924467 None:intergenic 35.0%
!! AGGCATGTTCTAAGAGTATA+TGG - chr1_2:4924515-4924534 Msa0050900:five_prime_UTR 35.0%
GGATGGATGTTTATATAAGG+TGG + chr1_2:4923151-4923170 None:intergenic 35.0%
!!! CTAGTTTTGGAGAAATGGTT+AGG + chr1_2:4923177-4923196 None:intergenic 35.0%
CTAACCATTTCTCCAAAACT+AGG - chr1_2:4923175-4923194 Msa0050900:three_prime_UTR 35.0%
!!! AAACCCTAGTTTTGGAGAAA+TGG + chr1_2:4923182-4923201 None:intergenic 35.0%
!! ATGTTTGCAGATTTAGCTTG+GGG + chr1_2:4923255-4923274 None:intergenic 35.0%
! CATGTTTGCAGATTTAGCTT+GGG + chr1_2:4923256-4923275 None:intergenic 35.0%
!!! CTTTCGCTTTTTGCTCATTT+GGG + chr1_2:4923283-4923302 None:intergenic 35.0%
TTTCTTCGCTGAATTGAATG+AGG - chr1_2:4923421-4923440 Msa0050900:three_prime_UTR 35.0%
! CAGGAAACTTAAACCTTTTC+TGG + chr1_2:4923680-4923699 None:intergenic 35.0%
TATAGTTCAACACTGTTCTC+AGG + chr1_2:4923699-4923718 None:intergenic 35.0%
!! TTGAGGTTTGTTATGGAAAG+TGG - chr1_2:4923825-4923844 Msa0050900:CDS 35.0%
CAATGTAATCCTTAACAGGT+TGG + chr1_2:4923944-4923963 None:intergenic 35.0%
GAGTCAATGTAATCCTTAAC+AGG + chr1_2:4923948-4923967 None:intergenic 35.0%
TTACCCTATAACAACCTTGT+TGG - chr1_2:4924006-4924025 Msa0050900:CDS 35.0%
CAAACCAACAAGGTTGTTAT+AGG + chr1_2:4924013-4924032 None:intergenic 35.0%
! CTATAACAACCTTGTTGGTT+TGG - chr1_2:4924011-4924030 Msa0050900:CDS 35.0%
ATGGTAACAATATCAGGTAG+GGG + chr1_2:4924160-4924179 None:intergenic 35.0%
GAATGGTAACAATATCAGGT+AGG + chr1_2:4924162-4924181 None:intergenic 35.0%
GTGTGAATGGTAACAATATC+AGG + chr1_2:4924166-4924185 None:intergenic 35.0%
TAATACACGGGAGGAAATTA+TGG + chr1_2:4924448-4924467 None:intergenic 35.0%
!! AGGCATGTTCTAAGAGTATA+TGG - chr1_2:4924515-4924534 Msa0050900:five_prime_UTR 35.0%
TTTGGAGAAATGGTTAGGGA+TGG + chr1_2:4923172-4923191 None:intergenic 40.0%
! GAAACTGCAAACCCTAGTTT+TGG + chr1_2:4923190-4923209 None:intergenic 40.0%
!! TGTTTGCAGATTTAGCTTGG+GGG + chr1_2:4923254-4923273 None:intergenic 40.0%
CCATGTTTGCAGATTTAGCT+TGG + chr1_2:4923257-4923276 None:intergenic 40.0%
CCAAGCTAAATCTGCAAACA+TGG - chr1_2:4923254-4923273 Msa0050900:three_prime_UTR 40.0%
!!! CCTTTCGCTTTTTGCTCATT+TGG + chr1_2:4923284-4923303 None:intergenic 40.0%
CCAAATGAGCAAAAAGCGAA+AGG - chr1_2:4923281-4923300 Msa0050900:three_prime_UTR 40.0%
AATGATTAGGTGAGAAGGGA+AGG - chr1_2:4923628-4923647 Msa0050900:CDS 40.0%
! AAACCTTTTCTGGACTACTG+AGG + chr1_2:4923670-4923689 None:intergenic 40.0%
TGTTGAACTATATGCCGAGA+AGG - chr1_2:4923707-4923726 Msa0050900:intron 40.0%
TCTCTTCGTTACAAGCTTCT+CGG - chr1_2:4923768-4923787 Msa0050900:CDS 40.0%
! TGTTATGGAAAGTGGTGCTA+AGG - chr1_2:4923833-4923852 Msa0050900:CDS 40.0%
! GTTATGGAAAGTGGTGCTAA+GGG - chr1_2:4923834-4923853 Msa0050900:CDS 40.0%
GGATGTGAGGTTATTGTTAG+TGG - chr1_2:4923855-4923874 Msa0050900:CDS 40.0%
ATCCTTGAACTTCATGGACT+TGG + chr1_2:4923901-4923920 None:intergenic 40.0%
AAGTCCATGAAGTTCAAGGA+TGG - chr1_2:4923900-4923919 Msa0050900:CDS 40.0%
ATAGCCATCCTTGAACTTCA+TGG + chr1_2:4923907-4923926 None:intergenic 40.0%
GATGGCTATATGATCTCATC+TGG - chr1_2:4923918-4923937 Msa0050900:CDS 40.0%
TAGACATGTTCTTCTCAGAC+AGG - chr1_2:4923974-4923993 Msa0050900:CDS 40.0%
! GTAGGGTGTTCTTGGTATTA+AGG - chr1_2:4924084-4924103 Msa0050900:CDS 40.0%
AGGTGAAGATTATGCTTGAC+TGG - chr1_2:4924104-4924123 Msa0050900:intron 40.0%
GGTGAAGATTATGCTTGACT+GGG - chr1_2:4924105-4924124 Msa0050900:intron 40.0%
TTAGGACCTTGCTTTCCTTT+AGG + chr1_2:4924133-4924152 None:intergenic 40.0%
! ATATCAGGTAGGGGAGTTTT+AGG + chr1_2:4924151-4924170 None:intergenic 40.0%
TTTCTCAAACAACAGGGACT+GGG + chr1_2:4924255-4924274 None:intergenic 40.0%
! TTTTCTCAAACAACAGGGAC+TGG + chr1_2:4924256-4924275 None:intergenic 40.0%
TAAGGAACATAATACACGGG+AGG + chr1_2:4924457-4924476 None:intergenic 40.0%
TTTGGAGAAATGGTTAGGGA+TGG + chr1_2:4923172-4923191 None:intergenic 40.0%
! GAAACTGCAAACCCTAGTTT+TGG + chr1_2:4923190-4923209 None:intergenic 40.0%
!! TGTTTGCAGATTTAGCTTGG+GGG + chr1_2:4923254-4923273 None:intergenic 40.0%
CCATGTTTGCAGATTTAGCT+TGG + chr1_2:4923257-4923276 None:intergenic 40.0%
CCAAGCTAAATCTGCAAACA+TGG - chr1_2:4923254-4923273 Msa0050900:three_prime_UTR 40.0%
!!! CCTTTCGCTTTTTGCTCATT+TGG + chr1_2:4923284-4923303 None:intergenic 40.0%
CCAAATGAGCAAAAAGCGAA+AGG - chr1_2:4923281-4923300 Msa0050900:three_prime_UTR 40.0%
AATGATTAGGTGAGAAGGGA+AGG - chr1_2:4923628-4923647 Msa0050900:CDS 40.0%
! AAACCTTTTCTGGACTACTG+AGG + chr1_2:4923670-4923689 None:intergenic 40.0%
TGTTGAACTATATGCCGAGA+AGG - chr1_2:4923707-4923726 Msa0050900:intron 40.0%
TCTCTTCGTTACAAGCTTCT+CGG - chr1_2:4923768-4923787 Msa0050900:CDS 40.0%
! TGTTATGGAAAGTGGTGCTA+AGG - chr1_2:4923833-4923852 Msa0050900:CDS 40.0%
! GTTATGGAAAGTGGTGCTAA+GGG - chr1_2:4923834-4923853 Msa0050900:CDS 40.0%
GGATGTGAGGTTATTGTTAG+TGG - chr1_2:4923855-4923874 Msa0050900:CDS 40.0%
ATCCTTGAACTTCATGGACT+TGG + chr1_2:4923901-4923920 None:intergenic 40.0%
AAGTCCATGAAGTTCAAGGA+TGG - chr1_2:4923900-4923919 Msa0050900:CDS 40.0%
ATAGCCATCCTTGAACTTCA+TGG + chr1_2:4923907-4923926 None:intergenic 40.0%
GATGGCTATATGATCTCATC+TGG - chr1_2:4923918-4923937 Msa0050900:CDS 40.0%
TAGACATGTTCTTCTCAGAC+AGG - chr1_2:4923974-4923993 Msa0050900:CDS 40.0%
! GTAGGGTGTTCTTGGTATTA+AGG - chr1_2:4924084-4924103 Msa0050900:CDS 40.0%
AGGTGAAGATTATGCTTGAC+TGG - chr1_2:4924104-4924123 Msa0050900:intron 40.0%
GGTGAAGATTATGCTTGACT+GGG - chr1_2:4924105-4924124 Msa0050900:intron 40.0%
TTAGGACCTTGCTTTCCTTT+AGG + chr1_2:4924133-4924152 None:intergenic 40.0%
! ATATCAGGTAGGGGAGTTTT+AGG + chr1_2:4924151-4924170 None:intergenic 40.0%
TTTCTCAAACAACAGGGACT+GGG + chr1_2:4924255-4924274 None:intergenic 40.0%
! TTTTCTCAAACAACAGGGAC+TGG + chr1_2:4924256-4924275 None:intergenic 40.0%
TAAGGAACATAATACACGGG+AGG + chr1_2:4924457-4924476 None:intergenic 40.0%
GAGAAATGGTTAGGGATGGA+TGG + chr1_2:4923168-4923187 None:intergenic 45.0%
!! TTTGCAGATTTAGCTTGGGG+GGG + chr1_2:4923252-4923271 None:intergenic 45.0%
!! GTTTGCAGATTTAGCTTGGG+GGG + chr1_2:4923253-4923272 None:intergenic 45.0%
CTTGCTGAAGATGGCTATTC+CGG - chr1_2:4923458-4923477 Msa0050900:three_prime_UTR 45.0%
! CTATTCCGGTGTTGAGGTTA+GGG - chr1_2:4923472-4923491 Msa0050900:exon 45.0%
AGTAACCCTAACCTCAACAC+CGG + chr1_2:4923480-4923499 None:intergenic 45.0%
ATGATGATCTCAGTGCGCAT+GGG + chr1_2:4923503-4923522 None:intergenic 45.0%
CTACGAACCAAGAACAGCTT+GGG + chr1_2:4923540-4923559 None:intergenic 45.0%
ACTACGAACCAAGAACAGCT+TGG + chr1_2:4923541-4923560 None:intergenic 45.0%
TGACCTCAGTAGTCCAGAAA+AGG - chr1_2:4923664-4923683 Msa0050900:intron 45.0%
ATGCCGAGAAGGTTAACAAC+AGG - chr1_2:4923718-4923737 Msa0050900:intron 45.0%
TGCCGAGAAGGTTAACAACA+GGG - chr1_2:4923719-4923738 Msa0050900:intron 45.0%
TCCCCTGTTGTTAACCTTCT+CGG + chr1_2:4923724-4923743 None:intergenic 45.0%
AAACCTCAACACACCGTAAC+AGG + chr1_2:4923814-4923833 None:intergenic 45.0%
!! CGGTGTGTTGAGGTTTGTTA+TGG - chr1_2:4923818-4923837 Msa0050900:CDS 45.0%
AGCCAAGTCCATGAAGTTCA+AGG - chr1_2:4923896-4923915 Msa0050900:CDS 45.0%
ATGCTTGACTGGGATCCTAA+AGG - chr1_2:4924115-4924134 Msa0050900:intron 45.0%
TGGGATCCTAAAGGAAAGCA+AGG - chr1_2:4924124-4924143 Msa0050900:intron 45.0%
TGTTACCATTCACACTCCCA+AGG - chr1_2:4924171-4924190 Msa0050900:intron 45.0%
ATGTACTCTTCCTCCTCCTT+GGG + chr1_2:4924190-4924209 None:intergenic 45.0%
TCATTTGCAACAACAGCAGC+AGG + chr1_2:4924217-4924236 None:intergenic 45.0%
GAGAAATGGTTAGGGATGGA+TGG + chr1_2:4923168-4923187 None:intergenic 45.0%
!! TTTGCAGATTTAGCTTGGGG+GGG + chr1_2:4923252-4923271 None:intergenic 45.0%
!! GTTTGCAGATTTAGCTTGGG+GGG + chr1_2:4923253-4923272 None:intergenic 45.0%
CTTGCTGAAGATGGCTATTC+CGG - chr1_2:4923458-4923477 Msa0050900:three_prime_UTR 45.0%
! CTATTCCGGTGTTGAGGTTA+GGG - chr1_2:4923472-4923491 Msa0050900:exon 45.0%
AGTAACCCTAACCTCAACAC+CGG + chr1_2:4923480-4923499 None:intergenic 45.0%
ATGATGATCTCAGTGCGCAT+GGG + chr1_2:4923503-4923522 None:intergenic 45.0%
CTACGAACCAAGAACAGCTT+GGG + chr1_2:4923540-4923559 None:intergenic 45.0%
ACTACGAACCAAGAACAGCT+TGG + chr1_2:4923541-4923560 None:intergenic 45.0%
TGACCTCAGTAGTCCAGAAA+AGG - chr1_2:4923664-4923683 Msa0050900:intron 45.0%
ATGCCGAGAAGGTTAACAAC+AGG - chr1_2:4923718-4923737 Msa0050900:intron 45.0%
TGCCGAGAAGGTTAACAACA+GGG - chr1_2:4923719-4923738 Msa0050900:intron 45.0%
TCCCCTGTTGTTAACCTTCT+CGG + chr1_2:4923724-4923743 None:intergenic 45.0%
AAACCTCAACACACCGTAAC+AGG + chr1_2:4923814-4923833 None:intergenic 45.0%
!! CGGTGTGTTGAGGTTTGTTA+TGG - chr1_2:4923818-4923837 Msa0050900:CDS 45.0%
AGCCAAGTCCATGAAGTTCA+AGG - chr1_2:4923896-4923915 Msa0050900:CDS 45.0%
ATGCTTGACTGGGATCCTAA+AGG - chr1_2:4924115-4924134 Msa0050900:intron 45.0%
TGGGATCCTAAAGGAAAGCA+AGG - chr1_2:4924124-4924143 Msa0050900:intron 45.0%
TGTTACCATTCACACTCCCA+AGG - chr1_2:4924171-4924190 Msa0050900:intron 45.0%
ATGTACTCTTCCTCCTCCTT+GGG + chr1_2:4924190-4924209 None:intergenic 45.0%
TCATTTGCAACAACAGCAGC+AGG + chr1_2:4924217-4924236 None:intergenic 45.0%
TTGTTGCAGTTCGTCGCTGA+CGG - chr1_2:4923395-4923414 Msa0050900:three_prime_UTR 50.0%
!! ACCAGAGAGCTTGCTGAAGA+TGG - chr1_2:4923449-4923468 Msa0050900:three_prime_UTR 50.0%
! AGATGGCTATTCCGGTGTTG+AGG - chr1_2:4923466-4923485 Msa0050900:exon 50.0%
! GCTATTCCGGTGTTGAGGTT+AGG - chr1_2:4923471-4923490 Msa0050900:exon 50.0%
GATGATGATCTCAGTGCGCA+TGG + chr1_2:4923504-4923523 None:intergenic 50.0%
ACTCGTACCCAAGCTGTTCT+TGG - chr1_2:4923530-4923549 Msa0050900:CDS 50.0%
GATTAGGTGAGAAGGGAAGG+AGG - chr1_2:4923631-4923650 Msa0050900:CDS 50.0%
GCCGAGAAGGTTAACAACAG+GGG - chr1_2:4923720-4923739 Msa0050900:intron 50.0%
CTTCGTTACAAGCTTCTCGG+AGG - chr1_2:4923771-4923790 Msa0050900:CDS 50.0%
! AAGTGGTGCTAAGGGATGTG+AGG - chr1_2:4923842-4923861 Msa0050900:CDS 50.0%
CTCATCTGGCCAACCTGTTA+AGG - chr1_2:4923932-4923951 Msa0050900:CDS 50.0%
TACCATTCACACTCCCAAGG+AGG - chr1_2:4924174-4924193 Msa0050900:intron 50.0%
GATGTACTCTTCCTCCTCCT+TGG + chr1_2:4924191-4924210 None:intergenic 50.0%
! AGGAGGAGGAAGAGTACATC+AGG - chr1_2:4924191-4924210 Msa0050900:intron 50.0%
AAACAACAGGGACTGGGACA+GGG + chr1_2:4924249-4924268 None:intergenic 50.0%
TTGTTGCAGTTCGTCGCTGA+CGG - chr1_2:4923395-4923414 Msa0050900:three_prime_UTR 50.0%
!! ACCAGAGAGCTTGCTGAAGA+TGG - chr1_2:4923449-4923468 Msa0050900:three_prime_UTR 50.0%
! AGATGGCTATTCCGGTGTTG+AGG - chr1_2:4923466-4923485 Msa0050900:exon 50.0%
! GCTATTCCGGTGTTGAGGTT+AGG - chr1_2:4923471-4923490 Msa0050900:exon 50.0%
GATGATGATCTCAGTGCGCA+TGG + chr1_2:4923504-4923523 None:intergenic 50.0%
ACTCGTACCCAAGCTGTTCT+TGG - chr1_2:4923530-4923549 Msa0050900:CDS 50.0%
GATTAGGTGAGAAGGGAAGG+AGG - chr1_2:4923631-4923650 Msa0050900:CDS 50.0%
GCCGAGAAGGTTAACAACAG+GGG - chr1_2:4923720-4923739 Msa0050900:intron 50.0%
CTTCGTTACAAGCTTCTCGG+AGG - chr1_2:4923771-4923790 Msa0050900:CDS 50.0%
! AAGTGGTGCTAAGGGATGTG+AGG - chr1_2:4923842-4923861 Msa0050900:CDS 50.0%
CTCATCTGGCCAACCTGTTA+AGG - chr1_2:4923932-4923951 Msa0050900:CDS 50.0%
TACCATTCACACTCCCAAGG+AGG - chr1_2:4924174-4924193 Msa0050900:intron 50.0%
GATGTACTCTTCCTCCTCCT+TGG + chr1_2:4924191-4924210 None:intergenic 50.0%
! AGGAGGAGGAAGAGTACATC+AGG - chr1_2:4924191-4924210 Msa0050900:intron 50.0%
AAACAACAGGGACTGGGACA+GGG + chr1_2:4924249-4924268 None:intergenic 50.0%
GCCATCTTCAGCAAGCTCTC+TGG + chr1_2:4923453-4923472 None:intergenic 55.0%
GCTGTTCGCAGAGCCTGTTA+CGG - chr1_2:4923798-4923817 Msa0050900:CDS 55.0%
GAGCCTGTTACGGTGTGTTG+AGG - chr1_2:4923808-4923827 Msa0050900:CDS 55.0%
! CTCCTCCTTGGGAGTGTGAA+TGG + chr1_2:4924179-4924198 None:intergenic 55.0%
CATTCACACTCCCAAGGAGG+AGG - chr1_2:4924177-4924196 Msa0050900:intron 55.0%
CAAACAACAGGGACTGGGAC+AGG + chr1_2:4924250-4924269 None:intergenic 55.0%
GCCATCTTCAGCAAGCTCTC+TGG + chr1_2:4923453-4923472 None:intergenic 55.0%
GCTGTTCGCAGAGCCTGTTA+CGG - chr1_2:4923798-4923817 Msa0050900:CDS 55.0%
GAGCCTGTTACGGTGTGTTG+AGG - chr1_2:4923808-4923827 Msa0050900:CDS 55.0%
! CTCCTCCTTGGGAGTGTGAA+TGG + chr1_2:4924179-4924198 None:intergenic 55.0%
CATTCACACTCCCAAGGAGG+AGG - chr1_2:4924177-4924196 Msa0050900:intron 55.0%
CAAACAACAGGGACTGGGAC+AGG + chr1_2:4924250-4924269 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 4923148 4924600 4923148 ID=Msa0050900;Name=Msa0050900
chr1_2 mRNA 4923148 4924600 4923148 ID=Msa0050900-mRNA-1;Parent=Msa0050900;Name=Msa0050900-mRNA-1;_AED=0.00;_eAED=0.00;_QI=125|1|1|1|1|1|4|330|239
chr1_2 exon 4923148 4923660 4923148 ID=Msa0050900-mRNA-1:exon:1348;Parent=Msa0050900-mRNA-1
chr1_2 exon 4923753 4924111 4923753 ID=Msa0050900-mRNA-1:exon:1347;Parent=Msa0050900-mRNA-1
chr1_2 exon 4924197 4924344 4924197 ID=Msa0050900-mRNA-1:exon:1346;Parent=Msa0050900-mRNA-1
chr1_2 exon 4924446 4924600 4924446 ID=Msa0050900-mRNA-1:exon:1345;Parent=Msa0050900-mRNA-1
chr1_2 five_prime_UTR 4924476 4924600 4924476 ID=Msa0050900-mRNA-1:five_prime_utr;Parent=Msa0050900-mRNA-1
chr1_2 CDS 4924446 4924475 4924446 ID=Msa0050900-mRNA-1:cds;Parent=Msa0050900-mRNA-1
chr1_2 CDS 4924197 4924344 4924197 ID=Msa0050900-mRNA-1:cds;Parent=Msa0050900-mRNA-1
chr1_2 CDS 4923753 4924111 4923753 ID=Msa0050900-mRNA-1:cds;Parent=Msa0050900-mRNA-1
chr1_2 CDS 4923478 4923660 4923478 ID=Msa0050900-mRNA-1:cds;Parent=Msa0050900-mRNA-1
chr1_2 three_prime_UTR 4923148 4923477 4923148 ID=Msa0050900-mRNA-1:three_prime_utr;Parent=Msa0050900-mRNA-1
Gene Sequence

>Msa0050900

ATGGCTACCCAAATGAGCAAAAAGCGAAAGTTCGTCGCTGACGGAGTTTTCTTCGCTGAATTGAATGAGGTTTTAACCAGAGAGCTTGCTGAAGATGGCTATTCCGGTGTTGAGGTTAGGGTTACTCCCATGCGCACTGAGATCATCATCAGAGCTACTCGTACCCAAGCTGTTCTTGGTGAGAAGGGAAGGAGGATCAGAGAACTGACCTCAGTAGTCCAGAAAAGGTTTAAGTTTCCTGAGAACAGTGTTGAACTATATGCCGAGAAGGTTAACAACAGGGGACTTTGTGCAATTGCACAAGCTGAGTCTCTTCGTTACAAGCTTCTCGGAGGTCTTGCTGTTCGCAGAGCCTGTTACGGTGTGTTGAGGTTTGTTATGGAAAGTGGTGCTAAGGGATGTGAGGTTATTGTTAGTGGAAAATTGAGAGCACAAAGAGCCAAGTCCATGAAGTTCAAGGATGGCTATATGATCTCATCTGGCCAACCTGTTAAGGATTACATTGACTCTGCTGTTAGACATGTTCTTCTCAGACAGGGTGTTCTTGGTATTAAGGTGAAGATTATGCTTGACTGGGATCCTAAAGGAAAGCAAGGTCCTAAAACTCCCCTACCTGATATTGTTACCATTCACACTCCCAAGGAGGAGGAAGAGTACATCAGGCCTGCTGCTGTTGTTGCAAATGATATTGAGGTCCCTGTCCCAGTCCCTGTTGTTTGA
Protein sequence

>Msa0050900

MATQMSKKRKFVADGVFFAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQAVLGEKGRRIRELTSVVQKRFKFPENSVELYAEKVNNRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMISSGQPVKDYIDSAVRHVLLRQGVLGIKVKIMLDWDPKGKQGPKTPLPDIVTIHTPKEEEEYIRPAAVVANDIEVPVPVPVV*