AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0053750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0053750 MtrunA17_Chr1g0150801 96.914 162 5 0 1 162 1 162 7.51e-118 329
Msa0053750 MtrunA17_Chr3g0129321 45.882 170 78 4 5 162 11 178 1.53e-36 125
Msa0053750 MtrunA17_Chr8g0383251 48.921 139 63 2 25 158 29 164 8.78e-36 122
Msa0053750 MtrunA17_Chr1g0194481 57.609 92 39 0 69 160 125 216 6.68e-32 116
Msa0053750 MtrunA17_Chr2g0294231 68.421 76 24 0 85 160 186 261 2.12e-31 115
Msa0053750 MtrunA17_Chr7g0270191 59.036 83 34 0 77 159 120 202 2.44e-29 108
Msa0053750 MtrunA17_Chr7g0224801 63.768 69 25 0 91 159 82 150 5.27e-29 104
Msa0053750 MtrunA17_Chr8g0386241 46.078 102 55 0 58 159 59 160 8.95e-29 104
Msa0053750 MtrunA17_Chr4g0051881 66.197 71 24 0 89 159 165 235 2.68e-28 107
Msa0053750 MtrunA17_Chr4g0068541 66.667 72 24 0 89 160 195 266 8.08e-28 107
Msa0053750 MtrunA17_Chr5g0445311 61.842 76 28 1 84 158 345 420 1.19e-27 107
Msa0053750 MtrunA17_Chr5g0445311 50.000 58 28 1 102 159 200 256 6.15e-12 62.4
Msa0053750 MtrunA17_Chr3g0106491 65.714 70 24 0 89 158 352 421 1.92e-27 106
Msa0053750 MtrunA17_Chr3g0106491 47.222 72 33 2 92 159 184 254 3.34e-16 74.7
Msa0053750 MtrunA17_Chr3g0101131 60.526 76 29 1 84 158 396 471 3.20e-27 106
Msa0053750 MtrunA17_Chr3g0101131 50.000 58 28 1 102 159 243 299 1.25e-12 64.7
Msa0053750 MtrunA17_Chr5g0443131 67.143 70 23 0 89 158 144 213 3.38e-27 103
Msa0053750 MtrunA17_Chr5g0418781 58.537 82 34 0 77 158 99 180 3.76e-27 102
Msa0053750 MtrunA17_Chr4g0004911 60.526 76 29 1 84 158 317 392 5.06e-27 105
Msa0053750 MtrunA17_Chr4g0004911 51.724 58 28 0 102 159 184 241 1.77e-15 72.8
Msa0053750 MtrunA17_Chr3g0129361 60.274 73 29 0 89 161 131 203 2.57e-26 99.4
Msa0053750 MtrunA17_Chr8g0335811 57.143 77 32 1 83 158 134 210 3.84e-26 99.0
Msa0053750 MtrunA17_Chr3g0079691 59.211 76 30 1 84 158 476 551 5.20e-26 103
Msa0053750 MtrunA17_Chr3g0079691 51.724 58 27 1 102 159 280 336 2.01e-14 69.7
Msa0053750 MtrunA17_Chr5g0432161 62.857 70 26 0 89 158 408 477 6.15e-26 102
Msa0053750 MtrunA17_Chr5g0432161 55.172 58 25 1 102 159 244 300 4.11e-15 71.6
Msa0053750 MtrunA17_Chr5g0447701 53.333 90 40 1 69 158 221 308 7.79e-26 101
Msa0053750 MtrunA17_Chr5g0447701 42.623 61 34 1 99 159 89 148 8.75e-11 59.3
Msa0053750 MtrunA17_Chr7g0249311 60.000 70 28 0 89 158 379 448 1.52e-25 101
Msa0053750 MtrunA17_Chr7g0249311 43.421 76 36 2 83 158 166 234 1.06e-12 64.7
Msa0053750 MtrunA17_Chr7g0248721 61.972 71 26 1 89 159 128 197 1.63e-25 97.1
Msa0053750 MtrunA17_Chr8g0383351 60.811 74 28 1 89 161 146 219 1.67e-25 97.4
Msa0053750 MtrunA17_Chr7g0249301 58.571 70 29 0 89 158 279 348 1.89e-25 100
Msa0053750 MtrunA17_Chr7g0249301 44.737 76 35 2 83 158 49 117 1.88e-12 63.9
Msa0053750 MtrunA17_Chr7g0243891 57.895 76 31 1 84 158 355 430 1.14e-24 99.0
Msa0053750 MtrunA17_Chr7g0243891 53.448 58 26 1 102 159 216 272 1.02e-14 70.5
Msa0053750 MtrunA17_Chr7g0218081 55.714 70 31 0 89 158 397 466 1.35e-24 98.6
Msa0053750 MtrunA17_Chr7g0218081 54.386 57 25 1 102 158 228 283 6.99e-14 68.2
Msa0053750 MtrunA17_Chr5g0418191 55.714 70 31 0 89 158 307 376 5.04e-24 96.7
Msa0053750 MtrunA17_Chr5g0418191 54.386 57 25 1 102 158 151 206 1.77e-13 67.0
Msa0053750 MtrunA17_Chr4g0075201 52.703 74 34 1 87 159 252 325 3.12e-23 94.7
Msa0053750 MtrunA17_Chr4g0042831 52.778 72 34 0 87 158 263 334 5.75e-23 93.2
Msa0053750 MtrunA17_Chr4g0042831 50.877 57 28 0 102 158 145 201 1.00e-13 67.4
Msa0053750 MtrunA17_Chr4g0074001 52.632 76 36 0 83 158 340 415 1.87e-22 92.8
Msa0053750 MtrunA17_Chr2g0288871 42.424 99 55 2 63 159 265 363 2.53e-22 92.4
Msa0053750 MtrunA17_Chr7g0270831 51.351 74 35 1 87 159 201 274 3.16e-22 91.7
Msa0053750 MtrunA17_Chr7g0238921 64.912 57 20 0 102 158 124 180 5.88e-22 89.7
Msa0053750 MtrunA17_Chr4g0051181 51.351 74 35 1 87 159 228 301 2.38e-21 89.4
Msa0053750 MtrunA17_Chr2g0322391 50.685 73 35 1 87 158 334 406 2.94e-21 89.4
Msa0053750 MtrunA17_Chr4g0025051 42.000 100 57 1 61 159 294 393 6.85e-21 88.2
Msa0053750 MtrunA17_Chr1g0189391 52.778 72 33 1 88 158 83 154 7.80e-21 87.0
Msa0053750 MtrunA17_Chr3g0099641 49.315 73 36 1 87 158 236 308 2.20e-20 86.7
Msa0053750 MtrunA17_Chr7g0216891 51.389 72 34 1 89 159 206 277 2.96e-20 86.7
Msa0053750 MtrunA17_Chr4g0053281 46.988 83 41 2 77 158 243 323 2.55e-19 82.8
Msa0053750 MtrunA17_Chr2g0333851 51.351 74 33 2 89 159 154 227 3.19e-19 82.4
Msa0053750 MtrunA17_Chr5g0401821 50.704 71 34 1 89 158 186 256 3.52e-19 81.6
Msa0053750 MtrunA17_Chr4g0060271 49.315 73 36 1 87 158 240 312 5.65e-19 82.8
Msa0053750 MtrunA17_Chr4g0025061 41.026 117 50 4 62 159 302 418 6.40e-19 82.8
Msa0053750 MtrunA17_Chr7g0263531 46.575 73 39 0 86 158 345 417 9.60e-19 82.0
Msa0053750 MtrunA17_Chr7g0263531 53.571 56 25 1 103 158 190 244 2.83e-13 66.2
Msa0053750 MtrunA17_Chr3g0136961 52.542 59 27 1 101 158 137 195 1.11e-18 80.5
Msa0053750 MtrunA17_Chr1g0147881 46.341 82 41 2 78 158 240 319 1.43e-18 80.9
Msa0053750 MtrunA17_Chr1g0188941 52.459 61 29 0 98 158 47 107 1.55e-18 76.3
Msa0053750 MtrunA17_Chr4g0001361 52.459 61 28 1 100 159 160 220 1.80e-18 80.5
Msa0053750 MtrunA17_Chr2g0302391 49.315 73 36 1 87 158 196 268 3.18e-18 80.5
Msa0053750 MtrunA17_Chr6g0485431 45.946 74 39 1 86 158 138 211 1.20e-17 78.6
Msa0053750 MtrunA17_Chr4g0071521 43.902 82 43 2 78 158 223 302 5.52e-17 76.3
Msa0053750 MtrunA17_Chr5g0447711 41.758 91 51 1 68 158 198 286 5.56e-17 76.3
Msa0053750 MtrunA17_Chr1g0184691 45.122 82 42 2 78 158 271 350 6.20e-17 76.6
Msa0053750 MtrunA17_Chr1g0150281 50.877 57 27 1 103 158 141 197 6.73e-17 75.5
Msa0053750 MtrunA17_Chr1g0150271 48.333 60 30 1 100 158 99 158 8.85e-17 75.1
Msa0053750 MtrunA17_Chr7g0228941 44.595 74 40 1 88 160 38 111 1.07e-16 72.0
Msa0053750 MtrunA17_Chr7g0270051 55.172 58 25 1 103 159 54 111 1.92e-16 74.3
Msa0053750 MtrunA17_Chr3g0139021 41.463 82 45 2 78 158 292 371 4.10e-16 74.3
Msa0053750 MtrunA17_Chr8g0348311 54.237 59 26 1 103 160 156 214 8.50e-16 73.2
Msa0053750 MtrunA17_Chr2g0293481 35.780 109 65 3 56 159 217 325 6.22e-15 71.2
Msa0053750 MtrunA17_Chr8g0379461 52.632 57 26 1 103 158 77 133 1.31e-14 68.9
Msa0053750 MtrunA17_Chr7g0248691 38.889 90 51 2 72 160 127 213 3.52e-14 68.6
Msa0053750 MtrunA17_Chr8g0387501 50.000 60 29 1 101 159 128 187 4.00e-14 67.8
Msa0053750 MtrunA17_Chr1g0202461 52.632 57 26 1 103 158 262 318 6.40e-14 68.2
Msa0053750 MtrunA17_Chr2g0284321 40.741 81 43 2 83 158 12 92 1.79e-13 65.5
Msa0053750 MtrunA17_Chr2g0319961 50.877 57 27 1 103 158 81 137 1.91e-13 65.9
Msa0053750 MtrunA17_Chr3g0126391 39.806 103 50 4 64 157 92 191 2.94e-13 66.2
Msa0053750 MtrunA17_Chr2g0289181 45.902 61 32 1 99 158 11 71 3.42e-13 65.1
Msa0053750 MtrunA17_Chr4g0024391 42.647 68 38 1 87 153 214 281 2.41e-12 63.5
Msa0053750 MtrunA17_Chr5g0432071 49.123 57 28 1 102 158 104 159 8.15e-12 60.8
Msa0053750 MtrunA17_Chr7g0244981 32.000 125 69 3 38 157 67 180 1.82e-11 60.8
Msa0053750 MtrunA17_Chr2g0315361 42.857 70 37 1 95 161 141 210 7.99e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0053750 AT5G26170.1 77.778 72 16 0 89 160 100 171 1.95e-39 132
Msa0053750 AT5G64810.1 43.871 155 75 4 18 160 14 168 1.03e-36 125
Msa0053750 AT5G49520.1 71.831 71 20 0 89 159 208 278 2.15e-30 114
Msa0053750 AT2G47260.1 60.714 84 33 0 77 160 149 232 3.74e-30 112
Msa0053750 AT1G29860.1 70.423 71 21 0 89 159 123 193 2.33e-29 108
Msa0053750 AT4G18170.1 63.636 77 28 0 83 159 153 229 3.71e-29 109
Msa0053750 AT5G13080.1 61.111 72 28 0 88 159 53 124 5.37e-29 104
Msa0053750 AT3G01970.1 62.857 70 26 0 89 158 52 121 8.76e-29 103
Msa0053750 AT4G26640.5 60.526 76 29 1 84 158 186 261 7.53e-28 107
Msa0053750 AT4G26640.5 50.000 60 29 1 100 159 33 91 1.61e-12 64.3
Msa0053750 AT4G26640.6 60.526 76 29 1 84 158 186 261 7.53e-28 107
Msa0053750 AT4G26640.6 50.000 60 29 1 100 159 33 91 1.61e-12 64.3
Msa0053750 AT4G26640.4 60.526 76 29 1 84 158 186 261 7.53e-28 107
Msa0053750 AT4G26640.4 50.000 60 29 1 100 159 33 91 1.61e-12 64.3
Msa0053750 AT4G26640.3 60.526 76 29 1 84 158 186 261 7.53e-28 107
Msa0053750 AT4G26640.3 50.000 60 29 1 100 159 33 91 1.61e-12 64.3
Msa0053750 AT3G62340.1 63.095 84 29 1 76 159 94 175 7.59e-28 105
Msa0053750 AT4G26640.7 60.526 76 29 1 84 158 322 397 9.56e-28 107
Msa0053750 AT4G26640.7 50.000 60 29 1 100 159 169 227 1.21e-12 65.1
Msa0053750 AT1G69310.4 63.636 77 27 1 83 158 127 203 1.01e-27 104
Msa0053750 AT1G69310.2 63.636 77 27 1 83 158 127 203 1.01e-27 104
Msa0053750 AT1G69310.1 63.636 77 27 1 83 158 127 203 1.01e-27 104
Msa0053750 AT4G26640.1 60.526 76 29 1 84 158 290 365 1.07e-27 107
Msa0053750 AT4G26640.1 50.000 60 29 1 100 159 137 195 1.31e-12 64.7
Msa0053750 AT4G26640.2 60.526 76 29 1 84 158 362 437 1.12e-27 107
Msa0053750 AT4G26640.2 50.000 60 29 1 100 159 209 267 1.14e-12 65.1
Msa0053750 AT2G38470.1 60.526 76 29 1 84 158 343 418 2.16e-27 107
Msa0053750 AT2G38470.1 51.724 58 27 1 102 159 184 240 1.99e-14 70.1
Msa0053750 AT5G07100.2 61.250 80 28 2 82 158 118 197 3.70e-27 101
Msa0053750 AT5G07100.2 48.276 58 30 0 102 159 24 81 2.82e-13 65.1
Msa0053750 AT5G07100.4 61.250 80 28 2 82 158 118 197 3.70e-27 101
Msa0053750 AT5G07100.4 48.276 58 30 0 102 159 24 81 2.82e-13 65.1
Msa0053750 AT5G07100.5 61.250 80 28 2 82 158 118 197 3.70e-27 101
Msa0053750 AT5G07100.5 48.276 58 30 0 102 159 24 81 2.82e-13 65.1
Msa0053750 AT5G07100.3 61.250 80 28 2 82 158 118 197 3.70e-27 101
Msa0053750 AT5G07100.3 48.276 58 30 0 102 159 24 81 2.82e-13 65.1
Msa0053750 AT5G07100.1 61.250 80 28 2 82 158 211 290 7.43e-27 103
Msa0053750 AT5G07100.1 48.276 58 30 0 102 159 117 174 3.61e-13 65.9
Msa0053750 AT4G39410.1 49.485 97 49 0 65 161 186 282 7.75e-27 102
Msa0053750 AT5G46350.1 63.380 71 26 0 89 159 170 240 7.90e-27 103
Msa0053750 AT2G44745.1 58.442 77 31 1 83 158 125 201 1.24e-26 100
Msa0053750 AT2G04880.2 61.429 70 27 0 89 158 270 339 1.42e-26 104
Msa0053750 AT2G04880.2 45.902 61 32 1 99 159 108 167 1.32e-11 62.0
Msa0053750 AT2G46130.1 59.740 77 31 0 83 159 11 87 1.96e-26 96.7
Msa0053750 AT2G04880.1 61.429 70 27 0 89 158 294 363 2.07e-26 103
Msa0053750 AT2G04880.1 45.902 61 32 1 99 159 108 167 1.36e-11 62.0
Msa0053750 AT1G64000.1 58.333 72 30 0 88 159 100 171 2.31e-26 99.0
Msa0053750 AT5G56270.1 59.211 76 30 1 84 158 468 543 4.75e-26 103
Msa0053750 AT5G56270.1 49.123 57 28 1 102 158 273 328 5.93e-12 63.2
Msa0053750 AT5G41570.1 54.217 83 31 1 84 159 73 155 1.05e-25 97.1
Msa0053750 AT4G26440.1 41.221 131 70 1 28 158 305 428 2.70e-25 101
Msa0053750 AT4G26440.1 34.783 115 62 4 45 158 131 233 1.34e-12 65.1
Msa0053750 AT2G21900.1 53.333 90 30 2 83 160 78 167 5.41e-25 95.9
Msa0053750 AT1G13960.2 55.714 70 31 0 89 158 243 312 2.53e-24 97.1
Msa0053750 AT1G13960.2 56.140 57 24 1 102 158 76 131 7.50e-15 70.9
Msa0053750 AT1G13960.1 55.714 70 31 0 89 158 396 465 3.30e-24 98.2
Msa0053750 AT1G13960.1 56.140 57 24 1 102 158 229 284 4.33e-15 72.0
Msa0053750 AT3G01080.2 55.714 70 31 0 89 158 331 400 5.66e-24 97.1
Msa0053750 AT3G01080.2 50.877 57 27 1 102 158 205 260 7.09e-13 65.5
Msa0053750 AT3G01080.1 55.714 70 31 0 89 158 293 362 6.43e-24 96.7
Msa0053750 AT3G01080.1 50.877 57 27 1 102 158 167 222 8.00e-13 65.5
Msa0053750 AT2G30250.1 56.579 76 32 1 84 158 309 384 1.81e-23 95.1
Msa0053750 AT2G30250.1 44.828 58 31 1 102 159 166 222 6.00e-11 60.1
Msa0053750 AT2G03340.1 54.286 70 32 0 89 158 402 471 6.02e-23 94.4
Msa0053750 AT2G03340.1 38.235 102 58 2 61 158 205 305 7.87e-15 71.2
Msa0053750 AT1G69310.3 59.740 77 26 2 83 158 127 199 1.03e-22 91.7
Msa0053750 AT4G04450.2 52.703 74 34 1 87 159 183 256 2.41e-22 92.4
Msa0053750 AT4G04450.1 52.703 74 34 1 87 159 277 350 6.35e-22 91.7
Msa0053750 AT4G22070.4 52.703 74 34 1 87 159 282 355 7.83e-22 91.3
Msa0053750 AT4G22070.3 52.703 74 34 1 87 159 282 355 7.83e-22 91.3
Msa0053750 AT4G22070.2 52.703 74 34 1 87 159 282 355 7.83e-22 91.3
Msa0053750 AT4G22070.1 52.703 74 34 1 87 159 282 355 7.83e-22 91.3
Msa0053750 AT1G62300.1 52.703 74 34 1 87 159 297 370 9.14e-22 91.3
Msa0053750 AT2G46130.2 55.844 77 30 1 83 159 11 83 1.93e-21 84.0
Msa0053750 AT4G30935.1 57.377 61 26 0 98 158 327 387 5.69e-21 88.6
Msa0053750 AT1G68150.1 51.351 74 35 1 87 159 220 293 1.01e-20 87.4
Msa0053750 AT4G01720.1 50.000 74 36 1 87 159 224 297 2.65e-20 87.0
Msa0053750 AT2G23320.1 41.000 100 58 1 60 158 198 297 3.09e-20 85.5
Msa0053750 AT5G15130.2 49.315 73 36 1 87 158 170 242 3.25e-20 86.7
Msa0053750 AT5G15130.1 49.315 73 36 1 87 158 212 284 3.69e-20 86.7
Msa0053750 AT1G80840.1 39.815 108 60 3 55 159 99 204 7.38e-20 84.3
Msa0053750 AT2G24570.1 43.137 102 51 3 62 158 201 300 8.79e-20 84.3
Msa0053750 AT1G55600.1 49.315 73 37 0 88 160 293 365 1.07e-19 85.1
Msa0053750 AT1G18860.1 47.297 74 38 1 87 159 224 297 1.46e-19 84.7
Msa0053750 AT4G31550.1 45.783 83 42 2 77 158 223 303 6.81e-19 82.0
Msa0053750 AT5G43290.1 57.627 59 25 0 100 158 112 170 1.58e-18 80.1
Msa0053750 AT4G24240.1 46.341 82 41 2 78 158 259 338 1.86e-18 80.9
Msa0053750 AT4G31550.2 45.122 82 42 2 78 158 223 302 1.94e-18 80.9
Msa0053750 AT4G31800.2 50.847 59 28 1 101 158 110 168 4.10e-18 78.6
Msa0053750 AT4G31800.1 50.847 59 28 1 101 158 175 233 6.47e-18 79.0
Msa0053750 AT3G04670.1 43.956 91 48 2 69 158 231 319 7.24e-18 79.3
Msa0053750 AT2G25000.2 50.847 59 28 1 101 158 102 160 7.57e-18 77.8
Msa0053750 AT2G25000.4 50.847 59 28 1 101 158 102 160 7.57e-18 77.8
Msa0053750 AT5G28650.1 47.561 82 40 2 78 158 240 319 1.14e-17 78.6
Msa0053750 AT2G25000.3 50.847 59 28 1 101 158 124 182 1.74e-17 77.0
Msa0053750 AT2G25000.1 50.847 59 28 1 101 158 145 203 2.10e-17 77.4
Msa0053750 AT2G30590.1 48.611 72 36 1 89 159 300 371 5.54e-17 77.0
Msa0053750 AT1G69810.2 41.053 95 54 2 67 159 110 204 1.28e-16 75.9
Msa0053750 AT1G69810.1 41.053 95 54 2 67 159 168 262 2.10e-16 75.5
Msa0053750 AT2G37260.3 40.385 104 59 2 58 158 222 325 2.33e-16 75.1
Msa0053750 AT2G37260.3 53.571 56 25 1 103 158 86 140 2.32e-12 63.9
Msa0053750 AT2G37260.2 40.385 104 59 2 58 158 220 323 2.40e-16 75.1
Msa0053750 AT2G37260.2 53.571 56 25 1 103 158 84 138 2.42e-12 63.9
Msa0053750 AT2G37260.1 40.385 104 59 2 58 158 302 405 2.99e-16 75.1
Msa0053750 AT2G37260.1 53.571 56 25 1 103 158 166 220 1.86e-12 64.3
Msa0053750 AT5G52830.1 58.621 58 23 1 103 159 166 223 4.34e-16 74.3
Msa0053750 AT1G29280.1 51.724 58 27 1 103 159 75 132 5.54e-16 73.2
Msa0053750 AT2G34830.2 36.290 124 70 4 41 159 163 282 7.58e-15 71.2
Msa0053750 AT2G34830.1 36.290 124 70 4 41 159 154 273 7.63e-15 71.2
Msa0053750 AT3G58710.2 53.448 58 26 1 103 159 71 128 2.34e-14 68.9
Msa0053750 AT3G58710.1 53.448 58 26 1 103 159 72 129 2.46e-14 68.9
Msa0053750 AT4G01250.1 50.847 59 28 1 103 160 129 187 2.49e-14 69.3
Msa0053750 AT1G30650.1 38.144 97 55 3 68 159 179 275 3.06e-14 69.7
Msa0053750 AT5G45050.1 36.449 107 55 3 60 159 1138 1238 1.11e-13 68.2
Msa0053750 AT5G45050.4 36.449 107 55 3 60 159 999 1099 1.11e-13 68.2
Msa0053750 AT5G45050.2 36.449 107 55 3 60 159 1110 1210 1.11e-13 68.2
Msa0053750 AT3G01080.3 50.877 57 27 1 102 158 205 260 3.63e-13 66.2
Msa0053750 AT4G23550.1 36.957 92 52 2 68 158 111 197 1.87e-12 63.9
Msa0053750 AT2G40740.1 38.554 83 48 1 78 157 149 231 4.92e-11 60.1
Msa0053750 AT2G40740.3 38.554 83 48 1 78 157 158 240 5.95e-11 59.7
Msa0053750 AT2G46400.1 35.556 90 49 2 77 157 73 162 7.47e-11 59.3

Find 44 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTACAATTTACCTTAAAA+AGG 0.152863 1_2:+8248625 Msa0053750:three_prime_UTR
CAATATATATACCTTGGATT+AGG 0.247704 1_2:-8248292 None:intergenic
ACCTCTGCTTTCTGCAAGTT+AGG 0.255682 1_2:+8248745 Msa0053750:three_prime_UTR
GATGATGAATTTGCTGTTCT+TGG 0.294438 1_2:-8247817 None:intergenic
TGGTTGCTAAAATCACTTTC+AGG 0.298136 1_2:-8247841 None:intergenic
AATATATATACCTTGGATTA+GGG 0.308746 1_2:-8248291 None:intergenic
GGGTACAGATGGAGGAAGTA+TGG 0.355517 1_2:+8248247 Msa0053750:CDS
TAATTGGAAGAAGGAAATTA+AGG 0.361306 1_2:+8248177 Msa0053750:CDS
ATATGAGTAGAAACGATAAT+TGG 0.361702 1_2:+8248161 Msa0053750:CDS
GTGATTGCAGGAATTATTAT+AGG 0.370210 1_2:+8248386 Msa0053750:intron
GAGGAAGTATGGAAAGAAAA+TGG 0.372428 1_2:+8248258 Msa0053750:CDS
CCTCTGCTTTCTGCAAGTTA+GGG 0.400644 1_2:+8248746 Msa0053750:three_prime_UTR
GTGGGTGTGCATGCCCTCAT+AGG 0.406915 1_2:-8248482 None:intergenic
TCTATATAACTGAATCTAGA+TGG 0.411414 1_2:-8248775 None:intergenic
GTTGAAAACTCAAACTTGTT+GGG 0.426445 1_2:-8248597 None:intergenic
TGTTTGAGCTAGCAAGAACT+TGG 0.437457 1_2:-8248505 None:intergenic
TGTATAACCATTTGCTGACT+AGG 0.443978 1_2:-8248689 None:intergenic
AGTTGAAAACTCAAACTTGT+TGG 0.477960 1_2:-8248598 None:intergenic
TATTATAGGTGTTCAGTTGA+GGG 0.484385 1_2:+8248400 Msa0053750:CDS
TGAGTTTCGATGACAATGAA+TGG 0.486049 1_2:+8247893 Msa0053750:CDS
TTTCAAGCCAACGAAGTAGC+AGG 0.492097 1_2:+8247949 Msa0053750:CDS
GAATTTGAATACTTTCATGT+AGG 0.502390 1_2:-8248575 None:intergenic
AAGTAGTCAGAGAGTTCAAA+TGG 0.505113 1_2:-8247871 None:intergenic
TGCAAAGCCTAGTCAGCAAA+TGG 0.505838 1_2:+8248682 Msa0053750:three_prime_UTR
GCTGCCACCTGCTACTTCGT+TGG 0.513271 1_2:-8247956 None:intergenic
GCAGGTGGCAGCTACATTGA+AGG 0.517291 1_2:+8247967 Msa0053750:CDS
AAAACTAAAGACTCAGTGGT+TGG 0.518786 1_2:-8247928 None:intergenic
TAGAAACGATAATTGGAAGA+AGG 0.519711 1_2:+8248168 Msa0053750:CDS
AGAGTTCAAATGGCCACTGC+TGG 0.523946 1_2:-8247861 None:intergenic
TTATTATAGGTGTTCAGTTG+AGG 0.524859 1_2:+8248399 Msa0053750:CDS
TTATGTGATAACAACCTATG+AGG 0.527879 1_2:+8248468 Msa0053750:CDS
CTGATTGAAATACTTGATGA+TGG 0.529008 1_2:+8248226 Msa0053750:CDS
TACTTGATGATGGGTACAGA+TGG 0.541635 1_2:+8248236 Msa0053750:CDS
GAGCTAGCAAGAACTTGGGT+GGG 0.555150 1_2:-8248500 None:intergenic
ATTATAGGTGTTCAGTTGAG+GGG 0.580189 1_2:+8248401 Msa0053750:CDS
CAGGTGGCAGCTACATTGAA+GGG 0.604012 1_2:+8247968 Msa0053750:CDS
TATGTGATAACAACCTATGA+GGG 0.610093 1_2:+8248469 Msa0053750:CDS
TGAGCTAGCAAGAACTTGGG+TGG 0.613536 1_2:-8248501 None:intergenic
TGATTGAAATACTTGATGAT+GGG 0.620660 1_2:+8248227 Msa0053750:CDS
GTTTGAGCTAGCAAGAACTT+GGG 0.621628 1_2:-8248504 None:intergenic
CCCTAACTTGCAGAAAGCAG+AGG 0.631582 1_2:-8248746 None:intergenic
TTGATGATGGGTACAGATGG+AGG 0.631832 1_2:+8248239 Msa0053750:CDS
TTAAGAACAGCCCTAATCCA+AGG 0.633554 1_2:+8248281 Msa0053750:CDS
CAAGCCAACGAAGTAGCAGG+TGG 0.667226 1_2:+8247952 Msa0053750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGTAATAAATTAATTTCA+TGG - chr1_2:8248323-8248342 None:intergenic 10.0%
!! TATGTAATAAATTAATTTCA+TGG - chr1_2:8248323-8248342 None:intergenic 10.0%
!!! TGTCTTTAACTTATAATTAT+TGG + chr1_2:8248098-8248117 Msa0053750:intron 15.0%
!!! TATATCTTATTCTGATTTTA+AGG - chr1_2:8248803-8248822 None:intergenic 15.0%
!!! TGTCTTTAACTTATAATTAT+TGG + chr1_2:8248098-8248117 Msa0053750:intron 15.0%
!!! TATATCTTATTCTGATTTTA+AGG - chr1_2:8248803-8248822 None:intergenic 15.0%
!! AATATATATACCTTGGATTA+GGG - chr1_2:8248294-8248313 None:intergenic 20.0%
!! ATGTACAATTTACCTTAAAA+AGG + chr1_2:8248625-8248644 Msa0053750:three_prime_UTR 20.0%
!! TGTACAATTTACCTTAAAAA+GGG + chr1_2:8248626-8248645 Msa0053750:three_prime_UTR 20.0%
!! AATATATATACCTTGGATTA+GGG - chr1_2:8248294-8248313 None:intergenic 20.0%
!! ATGTACAATTTACCTTAAAA+AGG + chr1_2:8248625-8248644 Msa0053750:three_prime_UTR 20.0%
!! TGTACAATTTACCTTAAAAA+GGG + chr1_2:8248626-8248645 Msa0053750:three_prime_UTR 20.0%
!! TAAGCCAATTTTTATCTTAC+CGG + chr1_2:8248008-8248027 Msa0053750:intron 25.0%
! ATATGAGTAGAAACGATAAT+TGG + chr1_2:8248161-8248180 Msa0053750:CDS 25.0%
! TAATTGGAAGAAGGAAATTA+AGG + chr1_2:8248177-8248196 Msa0053750:CDS 25.0%
!! TGATTGAAATACTTGATGAT+GGG + chr1_2:8248227-8248246 Msa0053750:CDS 25.0%
! CAATATATATACCTTGGATT+AGG - chr1_2:8248295-8248314 None:intergenic 25.0%
! CTAATCCAAGGTATATATAT+TGG + chr1_2:8248293-8248312 Msa0053750:intron 25.0%
! GTATTCCAATATATATACCT+TGG - chr1_2:8248301-8248320 None:intergenic 25.0%
! GAATTTGAATACTTTCATGT+AGG - chr1_2:8248578-8248597 None:intergenic 25.0%
!! AAAGTTCAAAACCCTTTTTA+AGG - chr1_2:8248640-8248659 None:intergenic 25.0%
! TCTATATAACTGAATCTAGA+TGG - chr1_2:8248778-8248797 None:intergenic 25.0%
!! TAAGCCAATTTTTATCTTAC+CGG + chr1_2:8248008-8248027 Msa0053750:intron 25.0%
! ATATGAGTAGAAACGATAAT+TGG + chr1_2:8248161-8248180 Msa0053750:CDS 25.0%
! TAATTGGAAGAAGGAAATTA+AGG + chr1_2:8248177-8248196 Msa0053750:CDS 25.0%
!! TGATTGAAATACTTGATGAT+GGG + chr1_2:8248227-8248246 Msa0053750:CDS 25.0%
! CAATATATATACCTTGGATT+AGG - chr1_2:8248295-8248314 None:intergenic 25.0%
! CTAATCCAAGGTATATATAT+TGG + chr1_2:8248293-8248312 Msa0053750:intron 25.0%
! GTATTCCAATATATATACCT+TGG - chr1_2:8248301-8248320 None:intergenic 25.0%
! GAATTTGAATACTTTCATGT+AGG - chr1_2:8248578-8248597 None:intergenic 25.0%
!! AAAGTTCAAAACCCTTTTTA+AGG - chr1_2:8248640-8248659 None:intergenic 25.0%
! TCTATATAACTGAATCTAGA+TGG - chr1_2:8248778-8248797 None:intergenic 25.0%
TTGAAAAACTAAAGACTCAG+TGG - chr1_2:8247935-8247954 None:intergenic 30.0%
AAGACCGGTAAGATAAAAAT+TGG - chr1_2:8248015-8248034 None:intergenic 30.0%
! TGTTACTGTTGTCATGTTTT+AGG + chr1_2:8248121-8248140 Msa0053750:intron 30.0%
TAGAAACGATAATTGGAAGA+AGG + chr1_2:8248168-8248187 Msa0053750:CDS 30.0%
CTGATTGAAATACTTGATGA+TGG + chr1_2:8248226-8248245 Msa0053750:CDS 30.0%
ATATGTATATGTGTGATTGC+AGG + chr1_2:8248374-8248393 Msa0053750:intron 30.0%
GTGATTGCAGGAATTATTAT+AGG + chr1_2:8248386-8248405 Msa0053750:intron 30.0%
TTATTATAGGTGTTCAGTTG+AGG + chr1_2:8248399-8248418 Msa0053750:CDS 30.0%
TATTATAGGTGTTCAGTTGA+GGG + chr1_2:8248400-8248419 Msa0053750:CDS 30.0%
!!! CTCTCAACTCTTTTTTTTAC+AGG - chr1_2:8248430-8248449 None:intergenic 30.0%
TTATGTGATAACAACCTATG+AGG + chr1_2:8248468-8248487 Msa0053750:CDS 30.0%
TATGTGATAACAACCTATGA+GGG + chr1_2:8248469-8248488 Msa0053750:CDS 30.0%
! GTTGAAAACTCAAACTTGTT+GGG - chr1_2:8248600-8248619 None:intergenic 30.0%
AGTTGAAAACTCAAACTTGT+TGG - chr1_2:8248601-8248620 None:intergenic 30.0%
!! TGGATTTTTCTCTGTCATTT+TGG - chr1_2:8247800-8247819 None:intergenic 30.0%
TTGAAAAACTAAAGACTCAG+TGG - chr1_2:8247935-8247954 None:intergenic 30.0%
AAGACCGGTAAGATAAAAAT+TGG - chr1_2:8248015-8248034 None:intergenic 30.0%
! TGTTACTGTTGTCATGTTTT+AGG + chr1_2:8248121-8248140 Msa0053750:intron 30.0%
TAGAAACGATAATTGGAAGA+AGG + chr1_2:8248168-8248187 Msa0053750:CDS 30.0%
CTGATTGAAATACTTGATGA+TGG + chr1_2:8248226-8248245 Msa0053750:CDS 30.0%
ATATGTATATGTGTGATTGC+AGG + chr1_2:8248374-8248393 Msa0053750:intron 30.0%
GTGATTGCAGGAATTATTAT+AGG + chr1_2:8248386-8248405 Msa0053750:intron 30.0%
TTATTATAGGTGTTCAGTTG+AGG + chr1_2:8248399-8248418 Msa0053750:CDS 30.0%
TATTATAGGTGTTCAGTTGA+GGG + chr1_2:8248400-8248419 Msa0053750:CDS 30.0%
!!! CTCTCAACTCTTTTTTTTAC+AGG - chr1_2:8248430-8248449 None:intergenic 30.0%
TTATGTGATAACAACCTATG+AGG + chr1_2:8248468-8248487 Msa0053750:CDS 30.0%
TATGTGATAACAACCTATGA+GGG + chr1_2:8248469-8248488 Msa0053750:CDS 30.0%
! GTTGAAAACTCAAACTTGTT+GGG - chr1_2:8248600-8248619 None:intergenic 30.0%
AGTTGAAAACTCAAACTTGT+TGG - chr1_2:8248601-8248620 None:intergenic 30.0%
!! GATGATGAATTTGCTGTTCT+TGG - chr1_2:8247820-8247839 None:intergenic 35.0%
TGGTTGCTAAAATCACTTTC+AGG - chr1_2:8247844-8247863 None:intergenic 35.0%
AAGTAGTCAGAGAGTTCAAA+TGG - chr1_2:8247874-8247893 None:intergenic 35.0%
TGAGTTTCGATGACAATGAA+TGG + chr1_2:8247893-8247912 Msa0053750:CDS 35.0%
AAAACTAAAGACTCAGTGGT+TGG - chr1_2:8247931-8247950 None:intergenic 35.0%
! GAGGAAGTATGGAAAGAAAA+TGG + chr1_2:8248258-8248277 Msa0053750:CDS 35.0%
ATTATAGGTGTTCAGTTGAG+GGG + chr1_2:8248401-8248420 Msa0053750:CDS 35.0%
! TGTATAACCATTTGCTGACT+AGG - chr1_2:8248692-8248711 None:intergenic 35.0%
!! GATGATGAATTTGCTGTTCT+TGG - chr1_2:8247820-8247839 None:intergenic 35.0%
TGGTTGCTAAAATCACTTTC+AGG - chr1_2:8247844-8247863 None:intergenic 35.0%
AAGTAGTCAGAGAGTTCAAA+TGG - chr1_2:8247874-8247893 None:intergenic 35.0%
TGAGTTTCGATGACAATGAA+TGG + chr1_2:8247893-8247912 Msa0053750:CDS 35.0%
AAAACTAAAGACTCAGTGGT+TGG - chr1_2:8247931-8247950 None:intergenic 35.0%
! GAGGAAGTATGGAAAGAAAA+TGG + chr1_2:8248258-8248277 Msa0053750:CDS 35.0%
ATTATAGGTGTTCAGTTGAG+GGG + chr1_2:8248401-8248420 Msa0053750:CDS 35.0%
! TGTATAACCATTTGCTGACT+AGG - chr1_2:8248692-8248711 None:intergenic 35.0%
!! TACTTGATGATGGGTACAGA+TGG + chr1_2:8248236-8248255 Msa0053750:CDS 40.0%
TTAAGAACAGCCCTAATCCA+AGG + chr1_2:8248281-8248300 Msa0053750:CDS 40.0%
GTTTGAGCTAGCAAGAACTT+GGG - chr1_2:8248507-8248526 None:intergenic 40.0%
TGTTTGAGCTAGCAAGAACT+TGG - chr1_2:8248508-8248527 None:intergenic 40.0%
!! TACTTGATGATGGGTACAGA+TGG + chr1_2:8248236-8248255 Msa0053750:CDS 40.0%
TTAAGAACAGCCCTAATCCA+AGG + chr1_2:8248281-8248300 Msa0053750:CDS 40.0%
GTTTGAGCTAGCAAGAACTT+GGG - chr1_2:8248507-8248526 None:intergenic 40.0%
TGTTTGAGCTAGCAAGAACT+TGG - chr1_2:8248508-8248527 None:intergenic 40.0%
!! GTGATTTTAGCAACCAGCAG+TGG + chr1_2:8247848-8247867 Msa0053750:CDS 45.0%
TTTCAAGCCAACGAAGTAGC+AGG + chr1_2:8247949-8247968 Msa0053750:CDS 45.0%
TGCACAAGACGAACAAAGAC+CGG - chr1_2:8248030-8248049 None:intergenic 45.0%
!! TTGATGATGGGTACAGATGG+AGG + chr1_2:8248239-8248258 Msa0053750:CDS 45.0%
TGCAAAGCCTAGTCAGCAAA+TGG + chr1_2:8248682-8248701 Msa0053750:three_prime_UTR 45.0%
ACCTCTGCTTTCTGCAAGTT+AGG + chr1_2:8248745-8248764 Msa0053750:three_prime_UTR 45.0%
CCTCTGCTTTCTGCAAGTTA+GGG + chr1_2:8248746-8248765 Msa0053750:three_prime_UTR 45.0%
!! GTGATTTTAGCAACCAGCAG+TGG + chr1_2:8247848-8247867 Msa0053750:CDS 45.0%
TTTCAAGCCAACGAAGTAGC+AGG + chr1_2:8247949-8247968 Msa0053750:CDS 45.0%
TGCACAAGACGAACAAAGAC+CGG - chr1_2:8248030-8248049 None:intergenic 45.0%
!! TTGATGATGGGTACAGATGG+AGG + chr1_2:8248239-8248258 Msa0053750:CDS 45.0%
TGCAAAGCCTAGTCAGCAAA+TGG + chr1_2:8248682-8248701 Msa0053750:three_prime_UTR 45.0%
ACCTCTGCTTTCTGCAAGTT+AGG + chr1_2:8248745-8248764 Msa0053750:three_prime_UTR 45.0%
CCTCTGCTTTCTGCAAGTTA+GGG + chr1_2:8248746-8248765 Msa0053750:three_prime_UTR 45.0%
!!! ATATACATAATTTTTTTAAA+CGG - chr1_2:8248078-8248097 None:intergenic 5.0%
!!! ATATACATAATTTTTTTAAA+CGG - chr1_2:8248078-8248097 None:intergenic 5.0%
AGAGTTCAAATGGCCACTGC+TGG - chr1_2:8247864-8247883 None:intergenic 50.0%
CAGGTGGCAGCTACATTGAA+GGG + chr1_2:8247968-8247987 Msa0053750:CDS 50.0%
GGGTACAGATGGAGGAAGTA+TGG + chr1_2:8248247-8248266 Msa0053750:CDS 50.0%
GAGCTAGCAAGAACTTGGGT+GGG - chr1_2:8248503-8248522 None:intergenic 50.0%
TGAGCTAGCAAGAACTTGGG+TGG - chr1_2:8248504-8248523 None:intergenic 50.0%
! CCCTAACTTGCAGAAAGCAG+AGG - chr1_2:8248749-8248768 None:intergenic 50.0%
AGAGTTCAAATGGCCACTGC+TGG - chr1_2:8247864-8247883 None:intergenic 50.0%
CAGGTGGCAGCTACATTGAA+GGG + chr1_2:8247968-8247987 Msa0053750:CDS 50.0%
GGGTACAGATGGAGGAAGTA+TGG + chr1_2:8248247-8248266 Msa0053750:CDS 50.0%
GAGCTAGCAAGAACTTGGGT+GGG - chr1_2:8248503-8248522 None:intergenic 50.0%
TGAGCTAGCAAGAACTTGGG+TGG - chr1_2:8248504-8248523 None:intergenic 50.0%
! CCCTAACTTGCAGAAAGCAG+AGG - chr1_2:8248749-8248768 None:intergenic 50.0%
CAAGCCAACGAAGTAGCAGG+TGG + chr1_2:8247952-8247971 Msa0053750:CDS 55.0%
GCAGGTGGCAGCTACATTGA+AGG + chr1_2:8247967-8247986 Msa0053750:CDS 55.0%
CAAGCCAACGAAGTAGCAGG+TGG + chr1_2:8247952-8247971 Msa0053750:CDS 55.0%
GCAGGTGGCAGCTACATTGA+AGG + chr1_2:8247967-8247986 Msa0053750:CDS 55.0%
! GCTGCCACCTGCTACTTCGT+TGG - chr1_2:8247959-8247978 None:intergenic 60.0%
GTGGGTGTGCATGCCCTCAT+AGG - chr1_2:8248485-8248504 None:intergenic 60.0%
! GCTGCCACCTGCTACTTCGT+TGG - chr1_2:8247959-8247978 None:intergenic 60.0%
GTGGGTGTGCATGCCCTCAT+AGG - chr1_2:8248485-8248504 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 8247798 8248836 8247798 ID=Msa0053750;Name=Msa0053750
chr1_2 mRNA 8247798 8248836 8247798 ID=Msa0053750-mRNA-1;Parent=Msa0053750;Name=Msa0053750-mRNA-1;_AED=0.05;_eAED=0.05;_QI=4|1|1|1|1|1|3|317|162
chr1_2 exon 8247798 8248006 8247798 ID=Msa0053750-mRNA-1:exon:2936;Parent=Msa0053750-mRNA-1
chr1_2 exon 8248143 8248302 8248143 ID=Msa0053750-mRNA-1:exon:2937;Parent=Msa0053750-mRNA-1
chr1_2 exon 8248396 8248836 8248396 ID=Msa0053750-mRNA-1:exon:2938;Parent=Msa0053750-mRNA-1
chr1_2 five_prime_UTR 8247798 8247801 8247798 ID=Msa0053750-mRNA-1:five_prime_utr;Parent=Msa0053750-mRNA-1
chr1_2 CDS 8247802 8248006 8247802 ID=Msa0053750-mRNA-1:cds;Parent=Msa0053750-mRNA-1
chr1_2 CDS 8248143 8248302 8248143 ID=Msa0053750-mRNA-1:cds;Parent=Msa0053750-mRNA-1
chr1_2 CDS 8248396 8248519 8248396 ID=Msa0053750-mRNA-1:cds;Parent=Msa0053750-mRNA-1
chr1_2 three_prime_UTR 8248520 8248836 8248520 ID=Msa0053750-mRNA-1:three_prime_utr;Parent=Msa0053750-mRNA-1
Gene Sequence

>Msa0053750

ATGACAGAGAAAAATCCAAGAACAGCAAATTCATCATCACCTGAAAGTGATTTTAGCAACCAGCAGTGGCCATTTGAACTCTCTGACTACTTGAGTTTCGATGACAATGAATGGCTACAAAACAATCCAACCACTGAGTCTTTAGTTTTTCAAGCCAACGAAGTAGCAGGTGGCAGCTACATTGAAGGGTCTTCTAGTTCAATCAGAGATACTAATAATATGAATATGAGTAGAAACGATAATTGGAAGAAGGAAATTAAGGAGAGAGTTGCATTCAAAACAAAGTCACTGATTGAAATACTTGATGATGGGTACAGATGGAGGAAGTATGGAAAGAAAATGGTTAAGAACAGCCCTAATCCAAGGAATTATTATAGGTGTTCAGTTGAGGGGTGTCCTGTAAAAAAAAGAGTTGAGAGAGATAATAATGATTCGAGTTATGTGATAACAACCTATGAGGGCATGCACACCCACCCAAGTTCTTGCTAG
Protein sequence

>Msa0053750

MTEKNPRTANSSSPESDFSNQQWPFELSDYLSFDDNEWLQNNPTTESLVFQANEVAGGSYIEGSSSSIRDTNNMNMSRNDNWKKEIKERVAFKTKSLIEILDDGYRWRKYGKKMVKNSPNPRNYYRCSVEGCPVKKRVERDNNDSSYVITTYEGMHTHPSSC*