AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0054430


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0054430 MtrunA17_Chr4g0024861 88.732 71 8 0 11 81 468 538 8.58e-41 139
Msa0054430 MtrunA17_Chr4g0024641 91.045 67 6 0 15 81 891 957 2.23e-38 134
Msa0054430 MtrunA17_Chr4g0024641 89.552 67 7 0 11 77 391 457 1.33e-36 129
Msa0054430 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 457 519 4.32e-36 128
Msa0054430 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 519 581 4.32e-36 128
Msa0054430 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 581 643 4.32e-36 128
Msa0054430 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 643 705 4.32e-36 128
Msa0054430 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 705 767 4.32e-36 128
Msa0054430 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 829 891 4.32e-36 128
Msa0054430 MtrunA17_Chr4g0024641 92.063 63 5 0 15 77 767 829 4.19e-35 125
Msa0054430 MtrunA17_Chr2g0322441 85.294 68 10 0 12 79 472 539 3.33e-37 130
Msa0054430 MtrunA17_Chr4g0038671 75.714 70 17 0 11 80 470 539 8.20e-32 115
Msa0054430 MtrunA17_Chr1g0157341 71.429 70 20 0 11 80 470 539 1.15e-31 115
Msa0054430 MtrunA17_Chr4g0075071 75.385 65 16 0 12 76 473 537 2.65e-31 114
Msa0054430 MtrunA17_Chr3g0143671 65.217 69 24 0 12 80 472 540 7.30e-28 104
Msa0054430 MtrunA17_Chr8g0374271 59.420 69 28 0 12 80 472 540 2.26e-22 89.0
Msa0054430 MtrunA17_Chr5g0402831 57.971 69 29 0 12 80 469 537 1.06e-21 87.0
Msa0054430 MtrunA17_Chr4g0043251 52.381 63 30 0 12 74 476 538 1.07e-17 75.5
Msa0054430 MtrunA17_Chr4g0056511 47.692 65 34 0 11 75 487 551 2.09e-16 72.0
Msa0054430 MtrunA17_Chr8g0374261 50.769 65 32 0 12 76 470 534 3.12e-16 71.6
Msa0054430 MtrunA17_Chr3g0121161 49.206 63 32 0 12 74 515 577 3.85e-15 68.6
Msa0054430 MtrunA17_Chr3g0121151 49.206 63 32 0 12 74 515 577 4.00e-15 68.6
Msa0054430 MtrunA17_Chr3g0121171 49.206 63 32 0 12 74 475 537 5.16e-15 68.2
Msa0054430 MtrunA17_Chr3g0121171 49.206 63 32 0 12 74 1050 1112 5.16e-15 68.2
Msa0054430 MtrunA17_Chr3g0121171 49.206 63 32 0 12 74 1625 1687 5.16e-15 68.2
Msa0054430 MtrunA17_Chr3g0121171 49.206 63 32 0 12 74 2191 2253 5.16e-15 68.2
Msa0054430 MtrunA17_Chr4g0043261 49.206 63 32 0 12 74 470 532 5.66e-15 67.8
Msa0054430 MtrunA17_Chr3g0079321 46.032 63 34 0 12 74 490 552 3.04e-13 63.2
Msa0054430 MtrunA17_Chr1g0197301 44.444 63 35 0 12 74 268 330 5.49e-13 62.0
Msa0054430 MtrunA17_Chr7g0234061 42.857 63 35 1 12 74 488 549 6.66e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0054430 AT1G21850.1 69.565 69 21 0 12 80 469 537 4.88e-30 110
Msa0054430 AT4G22010.1 67.143 70 23 0 11 80 468 537 1.75e-29 109
Msa0054430 AT4G38420.1 69.014 71 22 0 11 81 477 547 4.54e-29 108
Msa0054430 AT1G76160.1 72.308 65 18 0 12 76 469 533 4.87e-29 108
Msa0054430 AT1G41830.1 66.667 69 23 0 12 80 470 538 1.80e-28 106
Msa0054430 AT1G21860.1 68.116 69 22 0 12 80 469 537 2.37e-28 106
Msa0054430 AT4G28090.1 69.697 66 20 0 11 76 472 537 1.47e-27 103
Msa0054430 AT3G13400.2 60.317 63 25 0 12 74 476 538 2.13e-21 86.7
Msa0054430 AT3G13400.1 60.317 63 25 0 12 74 476 538 2.13e-21 86.7
Msa0054430 AT1G55560.1 58.730 63 26 0 12 74 475 537 5.34e-20 82.8
Msa0054430 AT1G55570.1 57.143 63 27 0 12 74 479 541 7.38e-20 82.0
Msa0054430 AT3G13390.1 58.730 63 26 0 12 74 478 540 7.74e-20 82.0
Msa0054430 AT5G66920.1 53.623 69 32 0 12 80 477 545 1.24e-18 78.6
Msa0054430 AT2G23630.2 50.769 65 32 0 12 76 476 540 6.19e-16 71.2
Msa0054430 AT2G23630.1 50.769 65 32 0 12 76 471 535 6.43e-16 70.9
Msa0054430 AT4G37160.1 50.769 65 32 0 12 76 473 537 7.52e-16 70.9
Msa0054430 AT1G75790.1 45.070 71 33 1 12 76 474 544 7.58e-15 67.8
Msa0054430 AT4G25240.2 45.312 64 35 0 11 74 488 551 3.29e-14 66.2
Msa0054430 AT4G25240.1 45.312 64 35 0 11 74 488 551 3.29e-14 66.2
Msa0054430 AT5G51480.1 43.750 64 36 0 11 74 488 551 1.17e-13 64.7
Msa0054430 AT4G12420.1 42.188 64 37 0 11 74 485 548 2.70e-12 60.8
Msa0054430 AT4G12420.2 42.188 64 37 0 11 74 485 548 2.70e-12 60.8
Msa0054430 AT5G48450.1 41.538 65 37 1 12 75 480 544 6.66e-12 59.7
Msa0054430 AT5G48450.2 40.909 66 38 1 11 75 479 544 1.03e-11 58.9

Find 24 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTGGGCAAGACAGTATT+TGG 0.172946 1_2:-9180580 Msa0054430:CDS
TGAAGTGCATTGCTTGTAAT+AGG 0.279583 1_2:+9180501 None:intergenic
AATACACTCTAAGATAAAAT+TGG 0.334224 1_2:+9180553 None:intergenic
CTTGTACTTTGAAACCTTGA+AGG 0.365098 1_2:-9181267 Msa0054430:intron
GTCCAGTCATTAAACCTTCA+AGG 0.380201 1_2:+9181253 None:intergenic
TCATCTCTCAATGAATTTGC+AGG 0.381836 1_2:+9180528 None:intergenic
GGAATGTGAGGTCTGAGAAT+TGG 0.449805 1_2:-9180596 Msa0054430:CDS
GAAGTGCATTGCTTGTAATA+GGG 0.454722 1_2:+9180502 None:intergenic
GAATGTGAGGTCTGAGAATT+GGG 0.454791 1_2:-9180595 Msa0054430:CDS
AATTGGGCAAGACAGTATTT+GGG 0.467279 1_2:-9180579 Msa0054430:CDS
TACTGTGCAGTCCATGACTT+TGG 0.507467 1_2:+9180645 None:intergenic
TTGCTACCAATTACTAATTG+TGG 0.521580 1_2:+9180457 None:intergenic
ACACTCTAAGATAAAATTGG+TGG 0.562579 1_2:+9180556 None:intergenic
GACTGCACAGTACATGCCAT+TGG 0.563858 1_2:-9180634 Msa0054430:CDS
GTACATGCCATTGGATAATG+TGG 0.568290 1_2:-9180625 Msa0054430:CDS
CACATTCCCACATTATCCAA+TGG 0.568508 1_2:+9180618 None:intergenic
CACAGGTGTATCCAAAGTCA+TGG 0.577502 1_2:-9180656 Msa0054430:intron
TCAAGGTTTCAAAGTACAAG+AGG 0.580792 1_2:+9181270 None:intergenic
ATGTGGGAATGTGGAATGTG+AGG 0.600216 1_2:-9180608 Msa0054430:CDS
CAATGCACTTCAGTGTGGCA+AGG 0.617833 1_2:-9180490 Msa0054430:CDS
TACATGCCATTGGATAATGT+GGG 0.658434 1_2:-9180624 Msa0054430:CDS
TCCTTATAAAATGAAAGACG+TGG 0.666691 1_2:-9181297 None:intergenic
CATTGGATAATGTGGGAATG+TGG 0.674160 1_2:-9180617 Msa0054430:CDS
ACAAGCAATGCACTTCAGTG+TGG 0.696188 1_2:-9180495 Msa0054430:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTAAATTCTATAGA+TGG - chr1_2:9180786-9180805 Msa0054430:intron 10.0%
!!! TTTTTTTTAAATTCTATAGA+TGG - chr1_2:9180786-9180805 Msa0054430:intron 10.0%
!!! TGGACATTTATTTTGATAAA+AGG - chr1_2:9180522-9180541 Msa0054430:CDS 20.0%
!!! GAAGATACTAATAATCATTT+TGG - chr1_2:9181039-9181058 Msa0054430:intron 20.0%
!!! AATACACTCTAAGATAAAAT+TGG + chr1_2:9181207-9181226 None:intergenic 20.0%
!!! TGGACATTTATTTTGATAAA+AGG - chr1_2:9180522-9180541 Msa0054430:CDS 20.0%
!!! GAAGATACTAATAATCATTT+TGG - chr1_2:9181039-9181058 Msa0054430:intron 20.0%
!!! AATACACTCTAAGATAAAAT+TGG + chr1_2:9181207-9181226 None:intergenic 20.0%
! GGACATTACATAATATATGT+TGG + chr1_2:9180670-9180689 None:intergenic 25.0%
! TAAATTTAACTCGATTGGTA+TGG + chr1_2:9180691-9180710 None:intergenic 25.0%
! TACCAATCGAGTTAAATTTA+TGG - chr1_2:9180691-9180710 Msa0054430:intron 25.0%
! ACCAATCGAGTTAAATTTAT+GGG - chr1_2:9180692-9180711 Msa0054430:intron 25.0%
! GGACATTACATAATATATGT+TGG + chr1_2:9180670-9180689 None:intergenic 25.0%
! TAAATTTAACTCGATTGGTA+TGG + chr1_2:9180691-9180710 None:intergenic 25.0%
! TACCAATCGAGTTAAATTTA+TGG - chr1_2:9180691-9180710 Msa0054430:intron 25.0%
! ACCAATCGAGTTAAATTTAT+GGG - chr1_2:9180692-9180711 Msa0054430:intron 25.0%
TCCCATAAATTTAACTCGAT+TGG + chr1_2:9180696-9180715 None:intergenic 30.0%
GTATGAACTATCTTGACAAA+TGG - chr1_2:9180736-9180755 Msa0054430:intron 30.0%
! AAAATTGAAGTACTAGTCCA+TGG + chr1_2:9180766-9180785 None:intergenic 30.0%
CTCTTGATATTAGATGTTAG+TGG - chr1_2:9180819-9180838 Msa0054430:intron 30.0%
! TTTGGCCTTTAACATGATTA+AGG - chr1_2:9181057-9181076 Msa0054430:intron 30.0%
! TTGGCCTTTAACATGATTAA+GGG - chr1_2:9181058-9181077 Msa0054430:intron 30.0%
CAAACCCTTAATCATGTTAA+AGG + chr1_2:9181065-9181084 None:intergenic 30.0%
GACTTTCATATTTCATACAC+AGG - chr1_2:9181084-9181103 Msa0054430:intron 30.0%
!! ACACTCTAAGATAAAATTGG+TGG + chr1_2:9181204-9181223 None:intergenic 30.0%
TCCCATAAATTTAACTCGAT+TGG + chr1_2:9180696-9180715 None:intergenic 30.0%
GTATGAACTATCTTGACAAA+TGG - chr1_2:9180736-9180755 Msa0054430:intron 30.0%
! AAAATTGAAGTACTAGTCCA+TGG + chr1_2:9180766-9180785 None:intergenic 30.0%
CTCTTGATATTAGATGTTAG+TGG - chr1_2:9180819-9180838 Msa0054430:intron 30.0%
! TTTGGCCTTTAACATGATTA+AGG - chr1_2:9181057-9181076 Msa0054430:intron 30.0%
! TTGGCCTTTAACATGATTAA+GGG - chr1_2:9181058-9181077 Msa0054430:intron 30.0%
CAAACCCTTAATCATGTTAA+AGG + chr1_2:9181065-9181084 None:intergenic 30.0%
GACTTTCATATTTCATACAC+AGG - chr1_2:9181084-9181103 Msa0054430:intron 30.0%
!! ACACTCTAAGATAAAATTGG+TGG + chr1_2:9181204-9181223 None:intergenic 30.0%
TCAAGGTTTCAAAGTACAAG+AGG + chr1_2:9180490-9180509 None:intergenic 35.0%
!! CTTGTACTTTGAAACCTTGA+AGG - chr1_2:9180490-9180509 Msa0054430:CDS 35.0%
AACCTTGAAGGTTTAATGAC+TGG - chr1_2:9180502-9180521 Msa0054430:CDS 35.0%
TACATGCCATTGGATAATGT+GGG - chr1_2:9181133-9181152 Msa0054430:intron 35.0%
AATTGGGCAAGACAGTATTT+GGG - chr1_2:9181178-9181197 Msa0054430:intron 35.0%
TCATCTCTCAATGAATTTGC+AGG + chr1_2:9181232-9181251 None:intergenic 35.0%
GAAGTGCATTGCTTGTAATA+GGG + chr1_2:9181258-9181277 None:intergenic 35.0%
TGAAGTGCATTGCTTGTAAT+AGG + chr1_2:9181259-9181278 None:intergenic 35.0%
TCAAGGTTTCAAAGTACAAG+AGG + chr1_2:9180490-9180509 None:intergenic 35.0%
!! CTTGTACTTTGAAACCTTGA+AGG - chr1_2:9180490-9180509 Msa0054430:CDS 35.0%
AACCTTGAAGGTTTAATGAC+TGG - chr1_2:9180502-9180521 Msa0054430:CDS 35.0%
TACATGCCATTGGATAATGT+GGG - chr1_2:9181133-9181152 Msa0054430:intron 35.0%
AATTGGGCAAGACAGTATTT+GGG - chr1_2:9181178-9181197 Msa0054430:intron 35.0%
TCATCTCTCAATGAATTTGC+AGG + chr1_2:9181232-9181251 None:intergenic 35.0%
GAAGTGCATTGCTTGTAATA+GGG + chr1_2:9181258-9181277 None:intergenic 35.0%
TGAAGTGCATTGCTTGTAAT+AGG + chr1_2:9181259-9181278 None:intergenic 35.0%
GTCCAGTCATTAAACCTTCA+AGG + chr1_2:9180507-9180526 None:intergenic 40.0%
! ATTGAAGTACTAGTCCATGG+TGG + chr1_2:9180763-9180782 None:intergenic 40.0%
GTACATGCCATTGGATAATG+TGG - chr1_2:9181132-9181151 Msa0054430:intron 40.0%
CACATTCCCACATTATCCAA+TGG + chr1_2:9181142-9181161 None:intergenic 40.0%
CATTGGATAATGTGGGAATG+TGG - chr1_2:9181140-9181159 Msa0054430:intron 40.0%
GAATGTGAGGTCTGAGAATT+GGG - chr1_2:9181162-9181181 Msa0054430:intron 40.0%
GAATTGGGCAAGACAGTATT+TGG - chr1_2:9181177-9181196 Msa0054430:intron 40.0%
GTCCAGTCATTAAACCTTCA+AGG + chr1_2:9180507-9180526 None:intergenic 40.0%
! ATTGAAGTACTAGTCCATGG+TGG + chr1_2:9180763-9180782 None:intergenic 40.0%
GTACATGCCATTGGATAATG+TGG - chr1_2:9181132-9181151 Msa0054430:intron 40.0%
CACATTCCCACATTATCCAA+TGG + chr1_2:9181142-9181161 None:intergenic 40.0%
CATTGGATAATGTGGGAATG+TGG - chr1_2:9181140-9181159 Msa0054430:intron 40.0%
GAATGTGAGGTCTGAGAATT+GGG - chr1_2:9181162-9181181 Msa0054430:intron 40.0%
GAATTGGGCAAGACAGTATT+TGG - chr1_2:9181177-9181196 Msa0054430:intron 40.0%
TCTTGACAAATGGTCCACCA+TGG - chr1_2:9180746-9180765 Msa0054430:intron 45.0%
CACAGGTGTATCCAAAGTCA+TGG - chr1_2:9181101-9181120 Msa0054430:intron 45.0%
TACTGTGCAGTCCATGACTT+TGG + chr1_2:9181115-9181134 None:intergenic 45.0%
ATGTGGGAATGTGGAATGTG+AGG - chr1_2:9181149-9181168 Msa0054430:intron 45.0%
GGAATGTGAGGTCTGAGAAT+TGG - chr1_2:9181161-9181180 Msa0054430:intron 45.0%
ACAAGCAATGCACTTCAGTG+TGG - chr1_2:9181262-9181281 Msa0054430:intron 45.0%
TCTTGACAAATGGTCCACCA+TGG - chr1_2:9180746-9180765 Msa0054430:intron 45.0%
CACAGGTGTATCCAAAGTCA+TGG - chr1_2:9181101-9181120 Msa0054430:intron 45.0%
TACTGTGCAGTCCATGACTT+TGG + chr1_2:9181115-9181134 None:intergenic 45.0%
ATGTGGGAATGTGGAATGTG+AGG - chr1_2:9181149-9181168 Msa0054430:intron 45.0%
GGAATGTGAGGTCTGAGAAT+TGG - chr1_2:9181161-9181180 Msa0054430:intron 45.0%
ACAAGCAATGCACTTCAGTG+TGG - chr1_2:9181262-9181281 Msa0054430:intron 45.0%
!!! TTTTTTTTTTATAAATTTGT+TGG - chr1_2:9180572-9180591 Msa0054430:CDS 5.0%
!!! TTTTTTTTTTATAAATTTGT+TGG - chr1_2:9180572-9180591 Msa0054430:CDS 5.0%
GACTGCACAGTACATGCCAT+TGG - chr1_2:9181123-9181142 Msa0054430:intron 50.0%
CAATGCACTTCAGTGTGGCA+AGG - chr1_2:9181267-9181286 Msa0054430:intron 50.0%
GACTGCACAGTACATGCCAT+TGG - chr1_2:9181123-9181142 Msa0054430:intron 50.0%
CAATGCACTTCAGTGTGGCA+AGG - chr1_2:9181267-9181286 Msa0054430:intron 50.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 9180470 9181309 9180470 ID=Msa0054430;Name=Msa0054430
chr1_2 mRNA 9180470 9181309 9180470 ID=Msa0054430-mRNA-1;Parent=Msa0054430;Name=Msa0054430-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0|0|0.5|1|1|2|0|81
chr1_2 exon 9181268 9181309 9181268 ID=Msa0054430-mRNA-1:exon:3311;Parent=Msa0054430-mRNA-1
chr1_2 exon 9180470 9180673 9180470 ID=Msa0054430-mRNA-1:exon:3310;Parent=Msa0054430-mRNA-1
chr1_2 CDS 9181268 9181309 9181268 ID=Msa0054430-mRNA-1:cds;Parent=Msa0054430-mRNA-1
chr1_2 CDS 9180470 9180673 9180470 ID=Msa0054430-mRNA-1:cds;Parent=Msa0054430-mRNA-1
Gene Sequence

>Msa0054430

ATGAAAGACGTGGCAGTCCTCTTGTACTTTGAAACCTTGAAGGTGTATCCAAAGTCATGGACTGCACAGTACATGCCATTGGATAATGTGGGAATGTGGAATGTGAGGTCTGAGAATTGGGCAAGACAGTATTTGGGCCACCAATTTTATCTTAGAGTGTATTCACCTGCAAATTCATTGAGAGATGAGTACCCTATTACAAGCAATGCACTTCAGTGTGGCAAGGCAGTGAGTCACCACAATTAG

Protein sequence

>Msa0054430

MKDVAVLLYFETLKVYPKSWTAQYMPLDNVGMWNVRSENWARQYLGHQFYLRVYSPANSLRDEYPITSNALQCGKAVSHHN*