AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0054470


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0054470 MtrunA17_Chr4g0024861 88.732 71 8 0 11 81 468 538 8.58e-41 139
Msa0054470 MtrunA17_Chr4g0024641 91.045 67 6 0 15 81 891 957 2.23e-38 134
Msa0054470 MtrunA17_Chr4g0024641 89.552 67 7 0 11 77 391 457 1.33e-36 129
Msa0054470 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 457 519 4.32e-36 128
Msa0054470 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 519 581 4.32e-36 128
Msa0054470 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 581 643 4.32e-36 128
Msa0054470 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 643 705 4.32e-36 128
Msa0054470 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 705 767 4.32e-36 128
Msa0054470 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 829 891 4.32e-36 128
Msa0054470 MtrunA17_Chr4g0024641 92.063 63 5 0 15 77 767 829 4.19e-35 125
Msa0054470 MtrunA17_Chr2g0322441 85.294 68 10 0 12 79 472 539 3.33e-37 130
Msa0054470 MtrunA17_Chr4g0038671 75.714 70 17 0 11 80 470 539 8.20e-32 115
Msa0054470 MtrunA17_Chr1g0157341 71.429 70 20 0 11 80 470 539 1.15e-31 115
Msa0054470 MtrunA17_Chr4g0075071 75.385 65 16 0 12 76 473 537 2.65e-31 114
Msa0054470 MtrunA17_Chr3g0143671 65.217 69 24 0 12 80 472 540 7.30e-28 104
Msa0054470 MtrunA17_Chr8g0374271 59.420 69 28 0 12 80 472 540 2.26e-22 89.0
Msa0054470 MtrunA17_Chr5g0402831 57.971 69 29 0 12 80 469 537 1.06e-21 87.0
Msa0054470 MtrunA17_Chr4g0043251 52.381 63 30 0 12 74 476 538 1.07e-17 75.5
Msa0054470 MtrunA17_Chr4g0056511 47.692 65 34 0 11 75 487 551 2.09e-16 72.0
Msa0054470 MtrunA17_Chr8g0374261 50.769 65 32 0 12 76 470 534 3.12e-16 71.6
Msa0054470 MtrunA17_Chr3g0121161 49.206 63 32 0 12 74 515 577 3.85e-15 68.6
Msa0054470 MtrunA17_Chr3g0121151 49.206 63 32 0 12 74 515 577 4.00e-15 68.6
Msa0054470 MtrunA17_Chr3g0121171 49.206 63 32 0 12 74 475 537 5.16e-15 68.2
Msa0054470 MtrunA17_Chr3g0121171 49.206 63 32 0 12 74 1050 1112 5.16e-15 68.2
Msa0054470 MtrunA17_Chr3g0121171 49.206 63 32 0 12 74 1625 1687 5.16e-15 68.2
Msa0054470 MtrunA17_Chr3g0121171 49.206 63 32 0 12 74 2191 2253 5.16e-15 68.2
Msa0054470 MtrunA17_Chr4g0043261 49.206 63 32 0 12 74 470 532 5.66e-15 67.8
Msa0054470 MtrunA17_Chr3g0079321 46.032 63 34 0 12 74 490 552 3.04e-13 63.2
Msa0054470 MtrunA17_Chr1g0197301 44.444 63 35 0 12 74 268 330 5.49e-13 62.0
Msa0054470 MtrunA17_Chr7g0234061 42.857 63 35 1 12 74 488 549 6.66e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0054470 AT1G21850.1 69.565 69 21 0 12 80 469 537 4.88e-30 110
Msa0054470 AT4G22010.1 67.143 70 23 0 11 80 468 537 1.75e-29 109
Msa0054470 AT4G38420.1 69.014 71 22 0 11 81 477 547 4.54e-29 108
Msa0054470 AT1G76160.1 72.308 65 18 0 12 76 469 533 4.87e-29 108
Msa0054470 AT1G41830.1 66.667 69 23 0 12 80 470 538 1.80e-28 106
Msa0054470 AT1G21860.1 68.116 69 22 0 12 80 469 537 2.37e-28 106
Msa0054470 AT4G28090.1 69.697 66 20 0 11 76 472 537 1.47e-27 103
Msa0054470 AT3G13400.2 60.317 63 25 0 12 74 476 538 2.13e-21 86.7
Msa0054470 AT3G13400.1 60.317 63 25 0 12 74 476 538 2.13e-21 86.7
Msa0054470 AT1G55560.1 58.730 63 26 0 12 74 475 537 5.34e-20 82.8
Msa0054470 AT1G55570.1 57.143 63 27 0 12 74 479 541 7.38e-20 82.0
Msa0054470 AT3G13390.1 58.730 63 26 0 12 74 478 540 7.74e-20 82.0
Msa0054470 AT5G66920.1 53.623 69 32 0 12 80 477 545 1.24e-18 78.6
Msa0054470 AT2G23630.2 50.769 65 32 0 12 76 476 540 6.19e-16 71.2
Msa0054470 AT2G23630.1 50.769 65 32 0 12 76 471 535 6.43e-16 70.9
Msa0054470 AT4G37160.1 50.769 65 32 0 12 76 473 537 7.52e-16 70.9
Msa0054470 AT1G75790.1 45.070 71 33 1 12 76 474 544 7.58e-15 67.8
Msa0054470 AT4G25240.2 45.312 64 35 0 11 74 488 551 3.29e-14 66.2
Msa0054470 AT4G25240.1 45.312 64 35 0 11 74 488 551 3.29e-14 66.2
Msa0054470 AT5G51480.1 43.750 64 36 0 11 74 488 551 1.17e-13 64.7
Msa0054470 AT4G12420.1 42.188 64 37 0 11 74 485 548 2.70e-12 60.8
Msa0054470 AT4G12420.2 42.188 64 37 0 11 74 485 548 2.70e-12 60.8
Msa0054470 AT5G48450.1 41.538 65 37 1 12 75 480 544 6.66e-12 59.7
Msa0054470 AT5G48450.2 40.909 66 38 1 11 75 479 544 1.03e-11 58.9

Find 24 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTGGGCAAGACAGTATT+TGG 0.172946 1_2:-9204158 Msa0054470:CDS
TGAAGTGCATTGCTTGTAAT+AGG 0.279583 1_2:+9204079 None:intergenic
AATACACTCTAAGATAAAAT+TGG 0.334224 1_2:+9204131 None:intergenic
CTTGTACTTTGAAACCTTGA+AGG 0.365098 1_2:-9204843 Msa0054470:intron
GTCCAGTCATTAAACCTTCA+AGG 0.380201 1_2:+9204829 None:intergenic
TCATCTCTCAATGAATTTGC+AGG 0.381836 1_2:+9204106 None:intergenic
GGAATGTGAGGTCTGAGAAT+TGG 0.449805 1_2:-9204174 Msa0054470:CDS
GAAGTGCATTGCTTGTAATA+GGG 0.454722 1_2:+9204080 None:intergenic
GAATGTGAGGTCTGAGAATT+GGG 0.454791 1_2:-9204173 Msa0054470:CDS
AATTGGGCAAGACAGTATTT+GGG 0.467279 1_2:-9204157 Msa0054470:CDS
TACTGTGCAGTCCATGACTT+TGG 0.507467 1_2:+9204223 None:intergenic
TTGCTACCAATTACTAATTG+TGG 0.521580 1_2:+9204035 None:intergenic
ACACTCTAAGATAAAATTGG+TGG 0.562579 1_2:+9204134 None:intergenic
GACTGCACAGTACATGCCAT+TGG 0.563858 1_2:-9204212 Msa0054470:CDS
GTACATGCCATTGGATAATG+TGG 0.568290 1_2:-9204203 Msa0054470:CDS
CACATTCCCACATTATCCAA+TGG 0.568508 1_2:+9204196 None:intergenic
CACAGGTGTATCCAAAGTCA+TGG 0.577502 1_2:-9204234 Msa0054470:intron
TCAAGGTTTCAAAGTACAAG+AGG 0.580792 1_2:+9204846 None:intergenic
ATGTGGGAATGTGGAATGTG+AGG 0.600216 1_2:-9204186 Msa0054470:CDS
CAATGCACTTCAGTGTGGCA+AGG 0.617833 1_2:-9204068 Msa0054470:CDS
TACATGCCATTGGATAATGT+GGG 0.658434 1_2:-9204202 Msa0054470:CDS
TCCTTATAAAATGAAAGACG+TGG 0.666691 1_2:-9204873 None:intergenic
CATTGGATAATGTGGGAATG+TGG 0.674160 1_2:-9204195 Msa0054470:CDS
ACAAGCAATGCACTTCAGTG+TGG 0.696188 1_2:-9204073 Msa0054470:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTAAATTCTATAGA+TGG - chr1_2:9204362-9204381 Msa0054470:intron 10.0%
!!! TTTTTTTTAAATTCTATAGA+TGG - chr1_2:9204362-9204381 Msa0054470:intron 10.0%
!!! TGGACATTTATTTTGATAAA+AGG - chr1_2:9204100-9204119 Msa0054470:CDS 20.0%
!!! GAAGATACTAATAATCATTT+TGG - chr1_2:9204615-9204634 Msa0054470:intron 20.0%
!!! AATACACTCTAAGATAAAAT+TGG + chr1_2:9204783-9204802 None:intergenic 20.0%
!!! TGGACATTTATTTTGATAAA+AGG - chr1_2:9204100-9204119 Msa0054470:CDS 20.0%
!!! GAAGATACTAATAATCATTT+TGG - chr1_2:9204615-9204634 Msa0054470:intron 20.0%
!!! AATACACTCTAAGATAAAAT+TGG + chr1_2:9204783-9204802 None:intergenic 20.0%
!!! TAGCTTTAAAGTGAATAAGT+TGG - chr1_2:9204197-9204216 Msa0054470:CDS 25.0%
!!! AGCTTTAAAGTGAATAAGTT+GGG - chr1_2:9204198-9204217 Msa0054470:CDS 25.0%
! GGACATTACATAATATATGT+TGG + chr1_2:9204246-9204265 None:intergenic 25.0%
! TAAATTTAACTCGATTGGTA+TGG + chr1_2:9204267-9204286 None:intergenic 25.0%
! TACCAATCGAGTTAAATTTA+TGG - chr1_2:9204267-9204286 Msa0054470:intron 25.0%
! ACCAATCGAGTTAAATTTAT+GGG - chr1_2:9204268-9204287 Msa0054470:intron 25.0%
!!! TAGCTTTAAAGTGAATAAGT+TGG - chr1_2:9204197-9204216 Msa0054470:CDS 25.0%
!!! AGCTTTAAAGTGAATAAGTT+GGG - chr1_2:9204198-9204217 Msa0054470:CDS 25.0%
! GGACATTACATAATATATGT+TGG + chr1_2:9204246-9204265 None:intergenic 25.0%
! TAAATTTAACTCGATTGGTA+TGG + chr1_2:9204267-9204286 None:intergenic 25.0%
! TACCAATCGAGTTAAATTTA+TGG - chr1_2:9204267-9204286 Msa0054470:intron 25.0%
! ACCAATCGAGTTAAATTTAT+GGG - chr1_2:9204268-9204287 Msa0054470:intron 25.0%
TCCCATAAATTTAACTCGAT+TGG + chr1_2:9204272-9204291 None:intergenic 30.0%
GTATGAACTATCTTGACAAA+TGG - chr1_2:9204312-9204331 Msa0054470:intron 30.0%
! AAAATTGAAGTACTAGTCCA+TGG + chr1_2:9204342-9204361 None:intergenic 30.0%
CTCTTGATATTAGATGTTAG+TGG - chr1_2:9204395-9204414 Msa0054470:intron 30.0%
! TTTGGCCTTTAACATGATTA+AGG - chr1_2:9204633-9204652 Msa0054470:intron 30.0%
! TTGGCCTTTAACATGATTAA+GGG - chr1_2:9204634-9204653 Msa0054470:intron 30.0%
CAAACCCTTAATCATGTTAA+AGG + chr1_2:9204641-9204660 None:intergenic 30.0%
GACTTTCATATTTCATACAC+AGG - chr1_2:9204660-9204679 Msa0054470:intron 30.0%
!! ACACTCTAAGATAAAATTGG+TGG + chr1_2:9204780-9204799 None:intergenic 30.0%
TCCCATAAATTTAACTCGAT+TGG + chr1_2:9204272-9204291 None:intergenic 30.0%
GTATGAACTATCTTGACAAA+TGG - chr1_2:9204312-9204331 Msa0054470:intron 30.0%
! AAAATTGAAGTACTAGTCCA+TGG + chr1_2:9204342-9204361 None:intergenic 30.0%
CTCTTGATATTAGATGTTAG+TGG - chr1_2:9204395-9204414 Msa0054470:intron 30.0%
! TTTGGCCTTTAACATGATTA+AGG - chr1_2:9204633-9204652 Msa0054470:intron 30.0%
! TTGGCCTTTAACATGATTAA+GGG - chr1_2:9204634-9204653 Msa0054470:intron 30.0%
CAAACCCTTAATCATGTTAA+AGG + chr1_2:9204641-9204660 None:intergenic 30.0%
GACTTTCATATTTCATACAC+AGG - chr1_2:9204660-9204679 Msa0054470:intron 30.0%
!! ACACTCTAAGATAAAATTGG+TGG + chr1_2:9204780-9204799 None:intergenic 30.0%
TCAAGGTTTCAAAGTACAAG+AGG + chr1_2:9204068-9204087 None:intergenic 35.0%
!! CTTGTACTTTGAAACCTTGA+AGG - chr1_2:9204068-9204087 Msa0054470:CDS 35.0%
AACCTTGAAGGTTTAATGAC+TGG - chr1_2:9204080-9204099 Msa0054470:CDS 35.0%
TACATGCCATTGGATAATGT+GGG - chr1_2:9204709-9204728 Msa0054470:intron 35.0%
AATTGGGCAAGACAGTATTT+GGG - chr1_2:9204754-9204773 Msa0054470:intron 35.0%
TCATCTCTCAATGAATTTGC+AGG + chr1_2:9204808-9204827 None:intergenic 35.0%
GAAGTGCATTGCTTGTAATA+GGG + chr1_2:9204834-9204853 None:intergenic 35.0%
TGAAGTGCATTGCTTGTAAT+AGG + chr1_2:9204835-9204854 None:intergenic 35.0%
TCAAGGTTTCAAAGTACAAG+AGG + chr1_2:9204068-9204087 None:intergenic 35.0%
!! CTTGTACTTTGAAACCTTGA+AGG - chr1_2:9204068-9204087 Msa0054470:CDS 35.0%
AACCTTGAAGGTTTAATGAC+TGG - chr1_2:9204080-9204099 Msa0054470:CDS 35.0%
TACATGCCATTGGATAATGT+GGG - chr1_2:9204709-9204728 Msa0054470:intron 35.0%
AATTGGGCAAGACAGTATTT+GGG - chr1_2:9204754-9204773 Msa0054470:intron 35.0%
TCATCTCTCAATGAATTTGC+AGG + chr1_2:9204808-9204827 None:intergenic 35.0%
GAAGTGCATTGCTTGTAATA+GGG + chr1_2:9204834-9204853 None:intergenic 35.0%
TGAAGTGCATTGCTTGTAAT+AGG + chr1_2:9204835-9204854 None:intergenic 35.0%
GTCCAGTCATTAAACCTTCA+AGG + chr1_2:9204085-9204104 None:intergenic 40.0%
! ATTGAAGTACTAGTCCATGG+TGG + chr1_2:9204339-9204358 None:intergenic 40.0%
GTACATGCCATTGGATAATG+TGG - chr1_2:9204708-9204727 Msa0054470:intron 40.0%
CACATTCCCACATTATCCAA+TGG + chr1_2:9204718-9204737 None:intergenic 40.0%
CATTGGATAATGTGGGAATG+TGG - chr1_2:9204716-9204735 Msa0054470:intron 40.0%
GAATGTGAGGTCTGAGAATT+GGG - chr1_2:9204738-9204757 Msa0054470:intron 40.0%
GAATTGGGCAAGACAGTATT+TGG - chr1_2:9204753-9204772 Msa0054470:intron 40.0%
GTCCAGTCATTAAACCTTCA+AGG + chr1_2:9204085-9204104 None:intergenic 40.0%
! ATTGAAGTACTAGTCCATGG+TGG + chr1_2:9204339-9204358 None:intergenic 40.0%
GTACATGCCATTGGATAATG+TGG - chr1_2:9204708-9204727 Msa0054470:intron 40.0%
CACATTCCCACATTATCCAA+TGG + chr1_2:9204718-9204737 None:intergenic 40.0%
CATTGGATAATGTGGGAATG+TGG - chr1_2:9204716-9204735 Msa0054470:intron 40.0%
GAATGTGAGGTCTGAGAATT+GGG - chr1_2:9204738-9204757 Msa0054470:intron 40.0%
GAATTGGGCAAGACAGTATT+TGG - chr1_2:9204753-9204772 Msa0054470:intron 40.0%
TCTTGACAAATGGTCCACCA+TGG - chr1_2:9204322-9204341 Msa0054470:intron 45.0%
CACAGGTGTATCCAAAGTCA+TGG - chr1_2:9204677-9204696 Msa0054470:intron 45.0%
TACTGTGCAGTCCATGACTT+TGG + chr1_2:9204691-9204710 None:intergenic 45.0%
ATGTGGGAATGTGGAATGTG+AGG - chr1_2:9204725-9204744 Msa0054470:intron 45.0%
GGAATGTGAGGTCTGAGAAT+TGG - chr1_2:9204737-9204756 Msa0054470:intron 45.0%
ACAAGCAATGCACTTCAGTG+TGG - chr1_2:9204838-9204857 Msa0054470:intron 45.0%
TCTTGACAAATGGTCCACCA+TGG - chr1_2:9204322-9204341 Msa0054470:intron 45.0%
CACAGGTGTATCCAAAGTCA+TGG - chr1_2:9204677-9204696 Msa0054470:intron 45.0%
TACTGTGCAGTCCATGACTT+TGG + chr1_2:9204691-9204710 None:intergenic 45.0%
ATGTGGGAATGTGGAATGTG+AGG - chr1_2:9204725-9204744 Msa0054470:intron 45.0%
GGAATGTGAGGTCTGAGAAT+TGG - chr1_2:9204737-9204756 Msa0054470:intron 45.0%
ACAAGCAATGCACTTCAGTG+TGG - chr1_2:9204838-9204857 Msa0054470:intron 45.0%
!!! TTTTTTTTTTATAAATTTGT+TGG - chr1_2:9204148-9204167 Msa0054470:CDS 5.0%
!!! TTTTTTTTTTATAAATTTGT+TGG - chr1_2:9204148-9204167 Msa0054470:CDS 5.0%
GACTGCACAGTACATGCCAT+TGG - chr1_2:9204699-9204718 Msa0054470:intron 50.0%
CAATGCACTTCAGTGTGGCA+AGG - chr1_2:9204843-9204862 Msa0054470:intron 50.0%
GACTGCACAGTACATGCCAT+TGG - chr1_2:9204699-9204718 Msa0054470:intron 50.0%
CAATGCACTTCAGTGTGGCA+AGG - chr1_2:9204843-9204862 Msa0054470:intron 50.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 9204048 9204885 9204048 ID=Msa0054470;Name=Msa0054470
chr1_2 mRNA 9204048 9204885 9204048 ID=Msa0054470-mRNA-1;Parent=Msa0054470;Name=Msa0054470-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0|0|0.5|1|1|2|0|81
chr1_2 exon 9204844 9204885 9204844 ID=Msa0054470-mRNA-1:exon:3422;Parent=Msa0054470-mRNA-1
chr1_2 exon 9204048 9204251 9204048 ID=Msa0054470-mRNA-1:exon:3421;Parent=Msa0054470-mRNA-1
chr1_2 CDS 9204844 9204885 9204844 ID=Msa0054470-mRNA-1:cds;Parent=Msa0054470-mRNA-1
chr1_2 CDS 9204048 9204251 9204048 ID=Msa0054470-mRNA-1:cds;Parent=Msa0054470-mRNA-1
Gene Sequence

>Msa0054470

ATGAAAGACGTGGCAGTCCTCTTGTACTTTGAAACCTTGAAGGTGTATCCAAAGTCATGGACTGCACAGTACATGCCATTGGATAATGTGGGAATGTGGAATGTGAGGTCTGAGAATTGGGCAAGACAGTATTTGGGCCACCAATTTTATCTTAGAGTGTATTCACCTGCAAATTCATTGAGAGATGAGTACCCTATTACAAGCAATGCACTTCAGTGTGGCAAGGCAGTGAGTCACCACAATTAG

Protein sequence

>Msa0054470

MKDVAVLLYFETLKVYPKSWTAQYMPLDNVGMWNVRSENWARQYLGHQFYLRVYSPANSLRDEYPITSNALQCGKAVSHHN*