AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0146780


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0146780 MtrunA17_Chr4g0024861 88.732 71 8 0 11 81 468 538 8.58e-41 139
Msa0146780 MtrunA17_Chr4g0024641 91.045 67 6 0 15 81 891 957 2.23e-38 134
Msa0146780 MtrunA17_Chr4g0024641 89.552 67 7 0 11 77 391 457 1.33e-36 129
Msa0146780 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 457 519 4.32e-36 128
Msa0146780 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 519 581 4.32e-36 128
Msa0146780 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 581 643 4.32e-36 128
Msa0146780 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 643 705 4.32e-36 128
Msa0146780 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 705 767 4.32e-36 128
Msa0146780 MtrunA17_Chr4g0024641 93.651 63 4 0 15 77 829 891 4.32e-36 128
Msa0146780 MtrunA17_Chr4g0024641 92.063 63 5 0 15 77 767 829 4.19e-35 125
Msa0146780 MtrunA17_Chr2g0322441 85.294 68 10 0 12 79 472 539 3.33e-37 130
Msa0146780 MtrunA17_Chr4g0038671 75.714 70 17 0 11 80 470 539 8.20e-32 115
Msa0146780 MtrunA17_Chr1g0157341 71.429 70 20 0 11 80 470 539 1.15e-31 115
Msa0146780 MtrunA17_Chr4g0075071 75.385 65 16 0 12 76 473 537 2.65e-31 114
Msa0146780 MtrunA17_Chr3g0143671 65.217 69 24 0 12 80 472 540 7.30e-28 104
Msa0146780 MtrunA17_Chr8g0374271 59.420 69 28 0 12 80 472 540 2.26e-22 89.0
Msa0146780 MtrunA17_Chr5g0402831 57.971 69 29 0 12 80 469 537 1.06e-21 87.0
Msa0146780 MtrunA17_Chr4g0043251 52.381 63 30 0 12 74 476 538 1.07e-17 75.5
Msa0146780 MtrunA17_Chr4g0056511 47.692 65 34 0 11 75 487 551 2.09e-16 72.0
Msa0146780 MtrunA17_Chr8g0374261 50.769 65 32 0 12 76 470 534 3.12e-16 71.6
Msa0146780 MtrunA17_Chr3g0121161 49.206 63 32 0 12 74 515 577 3.85e-15 68.6
Msa0146780 MtrunA17_Chr3g0121151 49.206 63 32 0 12 74 515 577 4.00e-15 68.6
Msa0146780 MtrunA17_Chr3g0121171 49.206 63 32 0 12 74 475 537 5.16e-15 68.2
Msa0146780 MtrunA17_Chr3g0121171 49.206 63 32 0 12 74 1050 1112 5.16e-15 68.2
Msa0146780 MtrunA17_Chr3g0121171 49.206 63 32 0 12 74 1625 1687 5.16e-15 68.2
Msa0146780 MtrunA17_Chr3g0121171 49.206 63 32 0 12 74 2191 2253 5.16e-15 68.2
Msa0146780 MtrunA17_Chr4g0043261 49.206 63 32 0 12 74 470 532 5.66e-15 67.8
Msa0146780 MtrunA17_Chr3g0079321 46.032 63 34 0 12 74 490 552 3.04e-13 63.2
Msa0146780 MtrunA17_Chr1g0197301 44.444 63 35 0 12 74 268 330 5.49e-13 62.0
Msa0146780 MtrunA17_Chr7g0234061 42.857 63 35 1 12 74 488 549 6.66e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0146780 AT1G21850.1 69.565 69 21 0 12 80 469 537 4.88e-30 110
Msa0146780 AT4G22010.1 67.143 70 23 0 11 80 468 537 1.75e-29 109
Msa0146780 AT4G38420.1 69.014 71 22 0 11 81 477 547 4.54e-29 108
Msa0146780 AT1G76160.1 72.308 65 18 0 12 76 469 533 4.87e-29 108
Msa0146780 AT1G41830.1 66.667 69 23 0 12 80 470 538 1.80e-28 106
Msa0146780 AT1G21860.1 68.116 69 22 0 12 80 469 537 2.37e-28 106
Msa0146780 AT4G28090.1 69.697 66 20 0 11 76 472 537 1.47e-27 103
Msa0146780 AT3G13400.2 60.317 63 25 0 12 74 476 538 2.13e-21 86.7
Msa0146780 AT3G13400.1 60.317 63 25 0 12 74 476 538 2.13e-21 86.7
Msa0146780 AT1G55560.1 58.730 63 26 0 12 74 475 537 5.34e-20 82.8
Msa0146780 AT1G55570.1 57.143 63 27 0 12 74 479 541 7.38e-20 82.0
Msa0146780 AT3G13390.1 58.730 63 26 0 12 74 478 540 7.74e-20 82.0
Msa0146780 AT5G66920.1 53.623 69 32 0 12 80 477 545 1.24e-18 78.6
Msa0146780 AT2G23630.2 50.769 65 32 0 12 76 476 540 6.19e-16 71.2
Msa0146780 AT2G23630.1 50.769 65 32 0 12 76 471 535 6.43e-16 70.9
Msa0146780 AT4G37160.1 50.769 65 32 0 12 76 473 537 7.52e-16 70.9
Msa0146780 AT1G75790.1 45.070 71 33 1 12 76 474 544 7.58e-15 67.8
Msa0146780 AT4G25240.2 45.312 64 35 0 11 74 488 551 3.29e-14 66.2
Msa0146780 AT4G25240.1 45.312 64 35 0 11 74 488 551 3.29e-14 66.2
Msa0146780 AT5G51480.1 43.750 64 36 0 11 74 488 551 1.17e-13 64.7
Msa0146780 AT4G12420.1 42.188 64 37 0 11 74 485 548 2.70e-12 60.8
Msa0146780 AT4G12420.2 42.188 64 37 0 11 74 485 548 2.70e-12 60.8
Msa0146780 AT5G48450.1 41.538 65 37 1 12 75 480 544 6.66e-12 59.7
Msa0146780 AT5G48450.2 40.909 66 38 1 11 75 479 544 1.03e-11 58.9

Find 24 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTGGGCAAGACAGTATT+TGG 0.172946 1_4:+6541409 Msa0146780:CDS
TGAAGTGCATTGCTTGTAAT+AGG 0.279583 1_4:-6541488 None:intergenic
AATACACTCTAAGATAAAAT+TGG 0.334224 1_4:-6541436 None:intergenic
CTTGTACTTTGAAACCTTGA+AGG 0.365098 1_4:+6540720 Msa0146780:CDS
GTCCAGTCATTAAACCTTCA+AGG 0.380201 1_4:-6540734 None:intergenic
TCATCTCTCAATGAATTTGC+AGG 0.381836 1_4:-6541461 None:intergenic
GGAATGTGAGGTCTGAGAAT+TGG 0.449805 1_4:+6541393 Msa0146780:CDS
GAAGTGCATTGCTTGTAATA+GGG 0.454722 1_4:-6541487 None:intergenic
GAATGTGAGGTCTGAGAATT+GGG 0.454791 1_4:+6541394 Msa0146780:CDS
AATTGGGCAAGACAGTATTT+GGG 0.467279 1_4:+6541410 Msa0146780:CDS
TACTGTGCAGTCCATGACTT+TGG 0.507467 1_4:-6541344 None:intergenic
TTGCTACCAATTACTAATTG+TGG 0.521580 1_4:-6541532 None:intergenic
ACACTCTAAGATAAAATTGG+TGG 0.562579 1_4:-6541433 None:intergenic
GACTGCACAGTACATGCCAT+TGG 0.563858 1_4:+6541355 Msa0146780:CDS
GTACATGCCATTGGATAATG+TGG 0.568290 1_4:+6541364 Msa0146780:CDS
CACATTCCCACATTATCCAA+TGG 0.568508 1_4:-6541371 None:intergenic
CACAGGTGTATCCAAAGTCA+TGG 0.577502 1_4:+6541333 Msa0146780:intron
TCAAGGTTTCAAAGTACAAG+AGG 0.580792 1_4:-6540717 None:intergenic
ATGTGGGAATGTGGAATGTG+AGG 0.600216 1_4:+6541381 Msa0146780:CDS
CAATGCACTTCAGTGTGGCA+AGG 0.617833 1_4:+6541499 Msa0146780:CDS
TACATGCCATTGGATAATGT+GGG 0.658434 1_4:+6541365 Msa0146780:CDS
CATTGGATAATGTGGGAATG+TGG 0.674160 1_4:+6541372 Msa0146780:CDS
ATAAAATAAAATGAAAGACG+TGG 0.692064 1_4:+6540690 None:intergenic
ACAAGCAATGCACTTCAGTG+TGG 0.696188 1_4:+6541494 Msa0146780:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTAAATTCTATAGA+TGG + chr1_4:6541018-6541037 Msa0146780:intron 10.0%
!!! TTTTTTTTAAATTCTATAGA+TGG + chr1_4:6541018-6541037 Msa0146780:intron 10.0%
!!! TGGACATTTATTTTGATAAA+AGG + chr1_4:6540752-6540771 Msa0146780:intron 20.0%
!!! TTAGCTTTAAATGAATAACT+TGG + chr1_4:6540854-6540873 Msa0146780:intron 20.0%
!!! TAGCTTTAAATGAATAACTT+GGG + chr1_4:6540855-6540874 Msa0146780:intron 20.0%
!!! GAAGATACTAATAATCATTT+TGG + chr1_4:6541271-6541290 Msa0146780:intron 20.0%
!!! AATACACTCTAAGATAAAAT+TGG - chr1_4:6541439-6541458 None:intergenic 20.0%
!!! TGGACATTTATTTTGATAAA+AGG + chr1_4:6540752-6540771 Msa0146780:intron 20.0%
!!! TTAGCTTTAAATGAATAACT+TGG + chr1_4:6540854-6540873 Msa0146780:intron 20.0%
!!! TAGCTTTAAATGAATAACTT+GGG + chr1_4:6540855-6540874 Msa0146780:intron 20.0%
!!! GAAGATACTAATAATCATTT+TGG + chr1_4:6541271-6541290 Msa0146780:intron 20.0%
!!! AATACACTCTAAGATAAAAT+TGG - chr1_4:6541439-6541458 None:intergenic 20.0%
! GGACATTACATAATATATGT+TGG - chr1_4:6540903-6540922 None:intergenic 25.0%
! TAAATTTAACTCGATTGGTA+TGG - chr1_4:6540924-6540943 None:intergenic 25.0%
! TACCAATCGAGTTAAATTTA+TGG + chr1_4:6540924-6540943 Msa0146780:intron 25.0%
! ACCAATCGAGTTAAATTTAT+GGG + chr1_4:6540925-6540944 Msa0146780:intron 25.0%
! GGACATTACATAATATATGT+TGG - chr1_4:6540903-6540922 None:intergenic 25.0%
! TAAATTTAACTCGATTGGTA+TGG - chr1_4:6540924-6540943 None:intergenic 25.0%
! TACCAATCGAGTTAAATTTA+TGG + chr1_4:6540924-6540943 Msa0146780:intron 25.0%
! ACCAATCGAGTTAAATTTAT+GGG + chr1_4:6540925-6540944 Msa0146780:intron 25.0%
TCCCATAAATTTAACTCGAT+TGG - chr1_4:6540929-6540948 None:intergenic 30.0%
GTATGAACTATCTTGACAAA+TGG + chr1_4:6540970-6540989 Msa0146780:intron 30.0%
! AAAATTGAAGTACTAGTCCA+TGG - chr1_4:6541000-6541019 None:intergenic 30.0%
! TTTGGCCTTTAACATGATTA+AGG + chr1_4:6541289-6541308 Msa0146780:intron 30.0%
! TTGGCCTTTAACATGATTAA+GGG + chr1_4:6541290-6541309 Msa0146780:intron 30.0%
CAAACCCTTAATCATGTTAA+AGG - chr1_4:6541297-6541316 None:intergenic 30.0%
GACTTTCATATTTCATACAC+AGG + chr1_4:6541316-6541335 Msa0146780:intron 30.0%
!! ACACTCTAAGATAAAATTGG+TGG - chr1_4:6541436-6541455 None:intergenic 30.0%
TCCCATAAATTTAACTCGAT+TGG - chr1_4:6540929-6540948 None:intergenic 30.0%
GTATGAACTATCTTGACAAA+TGG + chr1_4:6540970-6540989 Msa0146780:intron 30.0%
! AAAATTGAAGTACTAGTCCA+TGG - chr1_4:6541000-6541019 None:intergenic 30.0%
! TTTGGCCTTTAACATGATTA+AGG + chr1_4:6541289-6541308 Msa0146780:intron 30.0%
! TTGGCCTTTAACATGATTAA+GGG + chr1_4:6541290-6541309 Msa0146780:intron 30.0%
CAAACCCTTAATCATGTTAA+AGG - chr1_4:6541297-6541316 None:intergenic 30.0%
GACTTTCATATTTCATACAC+AGG + chr1_4:6541316-6541335 Msa0146780:intron 30.0%
!! ACACTCTAAGATAAAATTGG+TGG - chr1_4:6541436-6541455 None:intergenic 30.0%
TCAAGGTTTCAAAGTACAAG+AGG - chr1_4:6540720-6540739 None:intergenic 35.0%
!! CTTGTACTTTGAAACCTTGA+AGG + chr1_4:6540720-6540739 Msa0146780:CDS 35.0%
AACCTTGAAGGTTTAATGAC+TGG + chr1_4:6540732-6540751 Msa0146780:intron 35.0%
! CTCTTGATAGTAGATGTTAG+TGG + chr1_4:6541051-6541070 Msa0146780:intron 35.0%
TACATGCCATTGGATAATGT+GGG + chr1_4:6541365-6541384 Msa0146780:CDS 35.0%
AATTGGGCAAGACAGTATTT+GGG + chr1_4:6541410-6541429 Msa0146780:CDS 35.0%
TCATCTCTCAATGAATTTGC+AGG - chr1_4:6541464-6541483 None:intergenic 35.0%
GAAGTGCATTGCTTGTAATA+GGG - chr1_4:6541490-6541509 None:intergenic 35.0%
TGAAGTGCATTGCTTGTAAT+AGG - chr1_4:6541491-6541510 None:intergenic 35.0%
TCAAGGTTTCAAAGTACAAG+AGG - chr1_4:6540720-6540739 None:intergenic 35.0%
!! CTTGTACTTTGAAACCTTGA+AGG + chr1_4:6540720-6540739 Msa0146780:CDS 35.0%
AACCTTGAAGGTTTAATGAC+TGG + chr1_4:6540732-6540751 Msa0146780:intron 35.0%
! CTCTTGATAGTAGATGTTAG+TGG + chr1_4:6541051-6541070 Msa0146780:intron 35.0%
TACATGCCATTGGATAATGT+GGG + chr1_4:6541365-6541384 Msa0146780:CDS 35.0%
AATTGGGCAAGACAGTATTT+GGG + chr1_4:6541410-6541429 Msa0146780:CDS 35.0%
TCATCTCTCAATGAATTTGC+AGG - chr1_4:6541464-6541483 None:intergenic 35.0%
GAAGTGCATTGCTTGTAATA+GGG - chr1_4:6541490-6541509 None:intergenic 35.0%
TGAAGTGCATTGCTTGTAAT+AGG - chr1_4:6541491-6541510 None:intergenic 35.0%
GTCCAGTCATTAAACCTTCA+AGG - chr1_4:6540737-6540756 None:intergenic 40.0%
! ATTGAAGTACTAGTCCATGG+TGG - chr1_4:6540997-6541016 None:intergenic 40.0%
GTACATGCCATTGGATAATG+TGG + chr1_4:6541364-6541383 Msa0146780:CDS 40.0%
CACATTCCCACATTATCCAA+TGG - chr1_4:6541374-6541393 None:intergenic 40.0%
CATTGGATAATGTGGGAATG+TGG + chr1_4:6541372-6541391 Msa0146780:CDS 40.0%
GAATGTGAGGTCTGAGAATT+GGG + chr1_4:6541394-6541413 Msa0146780:CDS 40.0%
GAATTGGGCAAGACAGTATT+TGG + chr1_4:6541409-6541428 Msa0146780:CDS 40.0%
GTCCAGTCATTAAACCTTCA+AGG - chr1_4:6540737-6540756 None:intergenic 40.0%
! ATTGAAGTACTAGTCCATGG+TGG - chr1_4:6540997-6541016 None:intergenic 40.0%
GTACATGCCATTGGATAATG+TGG + chr1_4:6541364-6541383 Msa0146780:CDS 40.0%
CACATTCCCACATTATCCAA+TGG - chr1_4:6541374-6541393 None:intergenic 40.0%
CATTGGATAATGTGGGAATG+TGG + chr1_4:6541372-6541391 Msa0146780:CDS 40.0%
GAATGTGAGGTCTGAGAATT+GGG + chr1_4:6541394-6541413 Msa0146780:CDS 40.0%
GAATTGGGCAAGACAGTATT+TGG + chr1_4:6541409-6541428 Msa0146780:CDS 40.0%
TCTTGACAAATGGTCCACCA+TGG + chr1_4:6540980-6540999 Msa0146780:intron 45.0%
CACAGGTGTATCCAAAGTCA+TGG + chr1_4:6541333-6541352 Msa0146780:intron 45.0%
TACTGTGCAGTCCATGACTT+TGG - chr1_4:6541347-6541366 None:intergenic 45.0%
ATGTGGGAATGTGGAATGTG+AGG + chr1_4:6541381-6541400 Msa0146780:CDS 45.0%
GGAATGTGAGGTCTGAGAAT+TGG + chr1_4:6541393-6541412 Msa0146780:CDS 45.0%
ACAAGCAATGCACTTCAGTG+TGG + chr1_4:6541494-6541513 Msa0146780:CDS 45.0%
TCTTGACAAATGGTCCACCA+TGG + chr1_4:6540980-6540999 Msa0146780:intron 45.0%
CACAGGTGTATCCAAAGTCA+TGG + chr1_4:6541333-6541352 Msa0146780:intron 45.0%
TACTGTGCAGTCCATGACTT+TGG - chr1_4:6541347-6541366 None:intergenic 45.0%
ATGTGGGAATGTGGAATGTG+AGG + chr1_4:6541381-6541400 Msa0146780:CDS 45.0%
GGAATGTGAGGTCTGAGAAT+TGG + chr1_4:6541393-6541412 Msa0146780:CDS 45.0%
ACAAGCAATGCACTTCAGTG+TGG + chr1_4:6541494-6541513 Msa0146780:CDS 45.0%
!!! TTTATTTTTAATAAATTTGT+TGG + chr1_4:6540801-6540820 Msa0146780:intron 5.0%
!!! TTTATTTTTAATAAATTTGT+TGG + chr1_4:6540801-6540820 Msa0146780:intron 5.0%
GACTGCACAGTACATGCCAT+TGG + chr1_4:6541355-6541374 Msa0146780:CDS 50.0%
CAATGCACTTCAGTGTGGCA+AGG + chr1_4:6541499-6541518 Msa0146780:CDS 50.0%
GACTGCACAGTACATGCCAT+TGG + chr1_4:6541355-6541374 Msa0146780:CDS 50.0%
CAATGCACTTCAGTGTGGCA+AGG + chr1_4:6541499-6541518 Msa0146780:CDS 50.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 6540700 6541541 6540700 ID=Msa0146780;Name=Msa0146780
chr1_4 mRNA 6540700 6541541 6540700 ID=Msa0146780-mRNA-1;Parent=Msa0146780;Name=Msa0146780-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0|0|0.5|1|1|2|0|81
chr1_4 exon 6540700 6540741 6540700 ID=Msa0146780-mRNA-1:exon:2829;Parent=Msa0146780-mRNA-1
chr1_4 exon 6541338 6541541 6541338 ID=Msa0146780-mRNA-1:exon:2830;Parent=Msa0146780-mRNA-1
chr1_4 CDS 6540700 6540741 6540700 ID=Msa0146780-mRNA-1:cds;Parent=Msa0146780-mRNA-1
chr1_4 CDS 6541338 6541541 6541338 ID=Msa0146780-mRNA-1:cds;Parent=Msa0146780-mRNA-1
Gene Sequence

>Msa0146780

ATGAAAGACGTGGCAGTCCTCTTGTACTTTGAAACCTTGAAGGTGTATCCAAAGTCATGGACTGCACAGTACATGCCATTGGATAATGTGGGAATGTGGAATGTGAGGTCTGAGAATTGGGCAAGACAGTATTTGGGCCACCAATTTTATCTTAGAGTGTATTCACCTGCAAATTCATTGAGAGATGAGTACCCTATTACAAGCAATGCACTTCAGTGTGGCAAGGCAGTGAGTCACCACAATTAG

Protein sequence

>Msa0146780

MKDVAVLLYFETLKVYPKSWTAQYMPLDNVGMWNVRSENWARQYLGHQFYLRVYSPANSLRDEYPITSNALQCGKAVSHHN*