AlfalfaGEDB | Msa0058280

M. sativa cultivar zhongmu-4 / Msa0058280

KEGG: -
GO: GO:0001558 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006355 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0012505 GO:0016020 GO:0019219 GO:0019222 GO:0030308 GO:0030544 GO:0031072 GO:0031090 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031965 GO:0031967 GO:0031975 GO:0034641 GO:0034645 GO:0040008 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045893 GO:0045926 GO:0045934 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0061649 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0140030 GO:0140035 GO:0140036 GO:1901360 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001141
PFAM: PF00249.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0058280	XP_013466107.1	100.000	93	0	0	1	93	1	93	1.48e-62	194

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0058280	sp\|F4JVB8\|RADL1_ARATH	58.621	87	36	0	7	93	14	100	5.99e-35	117

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0058280	A0A072VEW4	100.000	93	0	0	1	93	1	93	7.08e-63	194

TFs/TRs:

Gene ID	Type	Classification
Msa0058280	TF	MYB-related

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003540	Msa0058280	0.961893	3.114795e-120	-8.615850e-47
Msa0058280	Msa0145790	0.966119	1.696737e-125	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0058280	MtrunA17_Chr1g0155181	100.000	86	0	0	1	86	1	86	1.59e-61	181
Msa0058280	MtrunA17_Chr3g0141601	73.404	94	21	2	1	93	1	91	8.20e-45	139
Msa0058280	MtrunA17_Chr3g0144631	73.134	67	18	0	5	71	14	80	2.21e-34	112
Msa0058280	MtrunA17_Chr3g0081631	57.447	94	33	3	1	93	4	91	2.51e-32	108
Msa0058280	MtrunA17_Chr7g0255841	57.647	85	30	1	9	93	14	92	1.43e-30	103
Msa0058280	MtrunA17_Chr3g0141641	47.253	91	45	1	6	93	2	92	9.66e-29	99.0
Msa0058280	MtrunA17_Chr5g0404101	65.217	69	24	0	2	70	14	82	1.82e-28	98.6
Msa0058280	MtrunA17_Chr6g0449111	58.571	70	29	0	2	71	5	74	3.15e-28	97.4
Msa0058280	MtrunA17_Chr8g0373111	55.224	67	29	1	4	70	2	67	6.22e-26	91.3
Msa0058280	MtrunA17_Chr8g0373121	55.224	67	29	1	4	70	2	67	6.22e-26	91.3
Msa0058280	MtrunA17_Chr8g0373131	55.224	67	29	1	4	70	2	67	6.25e-26	91.3
Msa0058280	MtrunA17_Chr8g0373101	56.338	71	30	1	4	74	2	71	4.04e-24	86.7
Msa0058280	MtrunA17_Chr5g0404301	61.905	63	23	1	5	67	2	63	5.85e-24	86.3
Msa0058280	MtrunA17_Chr1g0162081	50.602	83	41	0	1	83	6	88	2.61e-23	90.5
Msa0058280	MtrunA17_Chr8g0364741	53.623	69	32	0	1	69	4	72	5.25e-22	83.2
Msa0058280	MtrunA17_Chr8g0365011	57.576	66	28	0	4	69	5	70	1.73e-21	85.1
Msa0058280	MtrunA17_Chr5g0436501	55.882	68	30	0	2	69	22	89	1.75e-21	85.1
Msa0058280	MtrunA17_Chr3g0104071	52.113	71	34	0	4	74	40	110	2.85e-20	82.4
Msa0058280	MtrunA17_Chr5g0429521	51.389	72	34	1	4	75	26	96	3.16e-20	82.4
Msa0058280	MtrunA17_Chr7g0241761	50.000	72	36	0	2	73	30	101	1.35e-19	80.5
Msa0058280	MtrunA17_Chr4g0062741	48.000	75	35	2	1	71	1	75	1.12e-17	75.5
Msa0058280	MtrunA17_Chr1g0210471	42.254	71	41	0	7	77	24	94	1.84e-16	71.2
Msa0058280	MtrunA17_Chr1g0194071	49.153	59	29	1	5	62	9	67	2.13e-14	62.8
Msa0058280	MtrunA17_Chr7g0270711	47.619	63	32	1	3	64	7	69	1.80e-13	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0058280	AT4G39250.1	58.621	87	36	0	7	93	14	100	6.09e-36	117
Msa0058280	AT1G75250.1	72.464	69	19	0	2	70	7	75	2.12e-33	112
Msa0058280	AT1G75250.2	72.464	69	19	0	2	70	7	75	2.52e-33	111
Msa0058280	AT2G21650.1	55.682	88	38	1	7	93	14	101	1.28e-31	107
Msa0058280	AT1G19510.1	69.841	63	19	0	10	72	15	77	1.60e-28	99.0
Msa0058280	AT2G18328.1	65.079	63	22	0	10	72	14	76	5.40e-27	94.7
Msa0058280	AT5G05790.2	52.632	76	35	1	4	79	28	102	2.50e-21	85.1
Msa0058280	AT5G05790.1	52.632	76	35	1	4	79	28	102	2.50e-21	85.1
Msa0058280	AT5G58900.1	53.030	66	31	0	4	69	31	96	2.66e-20	82.4
Msa0058280	AT2G38090.1	55.556	63	28	0	7	69	29	91	3.30e-20	82.4
Msa0058280	AT3G11280.2	49.315	73	37	0	7	79	33	105	3.41e-19	79.3
Msa0058280	AT3G11280.1	49.315	73	37	0	7	79	33	105	3.41e-19	79.3
Msa0058280	AT4G36570.1	72.727	44	12	0	7	50	12	55	5.09e-19	73.9
Msa0058280	AT5G08520.1	43.590	78	44	0	1	78	6	83	1.16e-16	72.8
Msa0058280	AT1G49010.1	43.836	73	38	1	2	71	3	75	8.26e-16	70.9
Msa0058280	AT5G23650.1	46.377	69	37	0	1	69	7	75	5.00e-14	65.9
Msa0058280	AT5G01200.1	43.662	71	39	1	5	74	28	98	1.60e-13	63.9
Msa0058280	AT5G04760.1	40.299	67	39	1	3	69	2	67	6.58e-13	62.0

Find 36 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTTGCAGCATTTCTGTATT+TGG	0.079428	1_2:-14589364	None:intergenic
GCTAGCTTCTGCCACCTATC+TGG	0.154946	1_2:-14589243	None:intergenic
AGAAATGCTGCAACAATTAA+AGG	0.224298	1_2:+14589372	Msa0058280:CDS
TTGACAGAACGGATCCAATA+TGG	0.314973	1_2:+14590028	Msa0058280:three_prime_UTR
CTAGCTTCTGCCACCTATCT+GGG	0.322954	1_2:-14589242	None:intergenic
GTTGCAGCATTTCTGTATTT+GGG	0.335288	1_2:-14589363	None:intergenic
CAAAAGATTTGAGAATGCTT+TGG	0.369296	1_2:+14589200	Msa0058280:CDS
TGGGGTGTCCTTATCATAGA+TGG	0.425086	1_2:-14589223	None:intergenic
AACATAAACTTATGGTCAAA+TGG	0.434667	1_2:-14590275	None:intergenic
TTTGTAAGTCAATTATTTCA+AGG	0.441484	1_2:+14590205	Msa0058280:three_prime_UTR
TGAAGATTTGAAGCAAATTG+AGG	0.447717	1_2:+14589323	Msa0058280:CDS
TGCTTTGGCCATCTATGATA+AGG	0.453055	1_2:+14589215	Msa0058280:CDS
GATATATAAATGATAAATCA+TGG	0.500557	1_2:+14590252	Msa0058280:three_prime_UTR
CACGATGTATCTGTGCTGTA+TGG	0.501804	1_2:+14590167	Msa0058280:three_prime_UTR
CGTATATCATGCATCTAGCT+TGG	0.502919	1_2:+14590074	Msa0058280:three_prime_UTR
ATAGGTGGCAGAAGCTAGCA+AGG	0.504876	1_2:+14589247	Msa0058280:CDS
TTGTAAGTCAATTATTTCAA+GGG	0.512127	1_2:+14590206	Msa0058280:three_prime_UTR
CAGAAGCTAGCAAGGGCTGT+TGG	0.521879	1_2:+14589255	Msa0058280:CDS
GCATTTCTGTATTTGGGCAA+AGG	0.525051	1_2:-14589357	None:intergenic
ATGATAAGGACACCCCAGAT+AGG	0.538561	1_2:+14589229	Msa0058280:CDS
TTCTGTCAATACTTCACTGA+AGG	0.539972	1_2:-14590014	None:intergenic
TGACAGAACGGATCCAATAT+GGG	0.546105	1_2:+14590029	Msa0058280:three_prime_UTR
AAGCTAGCAAGGGCTGTTGG+AGG	0.552500	1_2:+14589258	Msa0058280:CDS
GAATGTCCTCAAGTTCAGCT+TGG	0.559569	1_2:+14589163	Msa0058280:exon
TATATATCATCATGATCAAG+TGG	0.560225	1_2:-14590237	None:intergenic
AGCTAGCAAGGGCTGTTGGA+GGG	0.566835	1_2:+14589259	Msa0058280:CDS
AGACAGTTGAGGAAGTGAAA+AGG	0.586038	1_2:+14589283	Msa0058280:CDS
TGTTGTCCAAGCTGAACTTG+AGG	0.595382	1_2:-14589169	None:intergenic
GATTTGAAGCAAATTGAGGA+AGG	0.610917	1_2:+14589327	Msa0058280:CDS
TAGCTTCTGCCACCTATCTG+GGG	0.629096	1_2:-14589241	None:intergenic
TCAGTGAAGTATTGACAGAA+CGG	0.642693	1_2:+14590017	Msa0058280:exon
TAGGTGGCAGAAGCTAGCAA+GGG	0.655725	1_2:+14589248	Msa0058280:CDS
ATAAGGACACCCCAGATAGG+TGG	0.660087	1_2:+14589232	Msa0058280:CDS
ATTACAGTGACGAAGAACAA+AGG	0.662329	1_2:+14589400	Msa0058280:CDS
TGTTGGAGGGAAGACAGTTG+AGG	0.663833	1_2:+14589272	Msa0058280:CDS
CAGAACGGATCCAATATGGG+TGG	0.678270	1_2:+14590032	Msa0058280:three_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATCTACAATTTTTTTTTTA+AGG	-	chr1_2:14589444-14589463	None:intergenic	10.0%
!!!	TATATTTTGTATATCTAATT+TGG	+	chr1_2:14589735-14589754	Msa0058280:intron	10.0%
!!!	TATCTACAATTTTTTTTTTA+AGG	-	chr1_2:14589444-14589463	None:intergenic	10.0%
!!!	TATATTTTGTATATCTAATT+TGG	+	chr1_2:14589735-14589754	Msa0058280:intron	10.0%
!!!	TTTTTTCACTAAATTTAATG+AGG	+	chr1_2:14589695-14589714	Msa0058280:intron	15.0%
!!!	TAATGTAGATTTTTATTACT+AGG	-	chr1_2:14589778-14589797	None:intergenic	15.0%
!!	GATATATAAATGATAAATCA+TGG	+	chr1_2:14590252-14590271	Msa0058280:three_prime_UTR	15.0%
!!!	TTTTTTCACTAAATTTAATG+AGG	+	chr1_2:14589695-14589714	Msa0058280:intron	15.0%
!!!	TAATGTAGATTTTTATTACT+AGG	-	chr1_2:14589778-14589797	None:intergenic	15.0%
!!	GATATATAAATGATAAATCA+TGG	+	chr1_2:14590252-14590271	Msa0058280:three_prime_UTR	15.0%
!!!	CTTCTTCTTGTTTATAAATA+TGG	+	chr1_2:14589921-14589940	Msa0058280:intron	20.0%
!!!	TCTAATCTAATATTGTTTTC+TGG	+	chr1_2:14590116-14590135	Msa0058280:three_prime_UTR	20.0%
!!	TTTGTAAGTCAATTATTTCA+AGG	+	chr1_2:14590205-14590224	Msa0058280:three_prime_UTR	20.0%
!!	TTGTAAGTCAATTATTTCAA+GGG	+	chr1_2:14590206-14590225	Msa0058280:three_prime_UTR	20.0%
!!!	CTTCTTCTTGTTTATAAATA+TGG	+	chr1_2:14589921-14589940	Msa0058280:intron	20.0%
!!!	TCTAATCTAATATTGTTTTC+TGG	+	chr1_2:14590116-14590135	Msa0058280:three_prime_UTR	20.0%
!!	TTTGTAAGTCAATTATTTCA+AGG	+	chr1_2:14590205-14590224	Msa0058280:three_prime_UTR	20.0%
!!	TTGTAAGTCAATTATTTCAA+GGG	+	chr1_2:14590206-14590225	Msa0058280:three_prime_UTR	20.0%
!!	GTTCGTCTTACTAATATTTT+TGG	+	chr1_2:14589534-14589553	Msa0058280:intron	25.0%
!	CGTATAAAACAGTAAGTTTA+TGG	-	chr1_2:14589884-14589903	None:intergenic	25.0%
!!	TATGGAGAAGATTTTTATTG+TGG	+	chr1_2:14589939-14589958	Msa0058280:intron	25.0%
!!	ATGGAGAAGATTTTTATTGT+GGG	+	chr1_2:14589940-14589959	Msa0058280:intron	25.0%
!	TATATATCATCATGATCAAG+TGG	-	chr1_2:14590240-14590259	None:intergenic	25.0%
!!	GTTCGTCTTACTAATATTTT+TGG	+	chr1_2:14589534-14589553	Msa0058280:intron	25.0%
!	CGTATAAAACAGTAAGTTTA+TGG	-	chr1_2:14589884-14589903	None:intergenic	25.0%
!!	TATGGAGAAGATTTTTATTG+TGG	+	chr1_2:14589939-14589958	Msa0058280:intron	25.0%
!!	ATGGAGAAGATTTTTATTGT+GGG	+	chr1_2:14589940-14589959	Msa0058280:intron	25.0%
!	TATATATCATCATGATCAAG+TGG	-	chr1_2:14590240-14590259	None:intergenic	25.0%
	CAAAAGATTTGAGAATGCTT+TGG	+	chr1_2:14589200-14589219	Msa0058280:CDS	30.0%
!	TGAAGATTTGAAGCAAATTG+AGG	+	chr1_2:14589323-14589342	Msa0058280:CDS	30.0%
	AGAAATGCTGCAACAATTAA+AGG	+	chr1_2:14589372-14589391	Msa0058280:CDS	30.0%
	AGTCATTAGAAACTCATACA+CGG	+	chr1_2:14589477-14589496	Msa0058280:intron	30.0%
	AGCAACACAAATTTAAAGAG+AGG	+	chr1_2:14589651-14589670	Msa0058280:intron	30.0%
	GTCTATTGTATTTGTAATGC+AGG	+	chr1_2:14589977-14589996	Msa0058280:intron	30.0%
!!!	TCTAATATTGTTTTCTGGCT+TGG	+	chr1_2:14590121-14590140	Msa0058280:three_prime_UTR	30.0%
	CAAAAGATTTGAGAATGCTT+TGG	+	chr1_2:14589200-14589219	Msa0058280:CDS	30.0%
!	TGAAGATTTGAAGCAAATTG+AGG	+	chr1_2:14589323-14589342	Msa0058280:CDS	30.0%
	AGAAATGCTGCAACAATTAA+AGG	+	chr1_2:14589372-14589391	Msa0058280:CDS	30.0%
	AGTCATTAGAAACTCATACA+CGG	+	chr1_2:14589477-14589496	Msa0058280:intron	30.0%
	AGCAACACAAATTTAAAGAG+AGG	+	chr1_2:14589651-14589670	Msa0058280:intron	30.0%
	GTCTATTGTATTTGTAATGC+AGG	+	chr1_2:14589977-14589996	Msa0058280:intron	30.0%
!!!	TCTAATATTGTTTTCTGGCT+TGG	+	chr1_2:14590121-14590140	Msa0058280:three_prime_UTR	30.0%
!	GATTTGAAGCAAATTGAGGA+AGG	+	chr1_2:14589327-14589346	Msa0058280:CDS	35.0%
	GTTGCAGCATTTCTGTATTT+GGG	-	chr1_2:14589366-14589385	None:intergenic	35.0%
	TGTTGCAGCATTTCTGTATT+TGG	-	chr1_2:14589367-14589386	None:intergenic	35.0%
	ATTACAGTGACGAAGAACAA+AGG	+	chr1_2:14589400-14589419	Msa0058280:CDS	35.0%
	TTCATTTACACACACCACAA+AGG	+	chr1_2:14589843-14589862	Msa0058280:intron	35.0%
	TTCTGTCAATACTTCACTGA+AGG	-	chr1_2:14590017-14590036	None:intergenic	35.0%
!!	TCAGTGAAGTATTGACAGAA+CGG	+	chr1_2:14590017-14590036	Msa0058280:exon	35.0%
!!!	CATTTTTTCTCCACCCATAT+TGG	-	chr1_2:14590045-14590064	None:intergenic	35.0%
!	GATTTGAAGCAAATTGAGGA+AGG	+	chr1_2:14589327-14589346	Msa0058280:CDS	35.0%
	GTTGCAGCATTTCTGTATTT+GGG	-	chr1_2:14589366-14589385	None:intergenic	35.0%
	TGTTGCAGCATTTCTGTATT+TGG	-	chr1_2:14589367-14589386	None:intergenic	35.0%
	ATTACAGTGACGAAGAACAA+AGG	+	chr1_2:14589400-14589419	Msa0058280:CDS	35.0%
	TTCATTTACACACACCACAA+AGG	+	chr1_2:14589843-14589862	Msa0058280:intron	35.0%
	TTCTGTCAATACTTCACTGA+AGG	-	chr1_2:14590017-14590036	None:intergenic	35.0%
!!	TCAGTGAAGTATTGACAGAA+CGG	+	chr1_2:14590017-14590036	Msa0058280:exon	35.0%
!!!	CATTTTTTCTCCACCCATAT+TGG	-	chr1_2:14590045-14590064	None:intergenic	35.0%
	TGCTTTGGCCATCTATGATA+AGG	+	chr1_2:14589215-14589234	Msa0058280:CDS	40.0%
	AGACAGTTGAGGAAGTGAAA+AGG	+	chr1_2:14589283-14589302	Msa0058280:CDS	40.0%
	GCATTTCTGTATTTGGGCAA+AGG	-	chr1_2:14589360-14589379	None:intergenic	40.0%
	AGTAAGTTTATGGTCCCTCA+TGG	-	chr1_2:14589874-14589893	None:intergenic	40.0%
	TTGACAGAACGGATCCAATA+TGG	+	chr1_2:14590028-14590047	Msa0058280:three_prime_UTR	40.0%
	TGACAGAACGGATCCAATAT+GGG	+	chr1_2:14590029-14590048	Msa0058280:three_prime_UTR	40.0%
	CGTATATCATGCATCTAGCT+TGG	+	chr1_2:14590074-14590093	Msa0058280:three_prime_UTR	40.0%
	TGTGTGAATGAATGAGCCAA+TGG	-	chr1_2:14589086-14589105	None:intergenic	40.0%
	TGCTTTGGCCATCTATGATA+AGG	+	chr1_2:14589215-14589234	Msa0058280:CDS	40.0%
	AGACAGTTGAGGAAGTGAAA+AGG	+	chr1_2:14589283-14589302	Msa0058280:CDS	40.0%
	GCATTTCTGTATTTGGGCAA+AGG	-	chr1_2:14589360-14589379	None:intergenic	40.0%
	AGTAAGTTTATGGTCCCTCA+TGG	-	chr1_2:14589874-14589893	None:intergenic	40.0%
	TTGACAGAACGGATCCAATA+TGG	+	chr1_2:14590028-14590047	Msa0058280:three_prime_UTR	40.0%
	TGACAGAACGGATCCAATAT+GGG	+	chr1_2:14590029-14590048	Msa0058280:three_prime_UTR	40.0%
	CGTATATCATGCATCTAGCT+TGG	+	chr1_2:14590074-14590093	Msa0058280:three_prime_UTR	40.0%
	GAATGTCCTCAAGTTCAGCT+TGG	+	chr1_2:14589163-14589182	Msa0058280:exon	45.0%
	TGTTGTCCAAGCTGAACTTG+AGG	-	chr1_2:14589172-14589191	None:intergenic	45.0%
!	TGGGGTGTCCTTATCATAGA+TGG	-	chr1_2:14589226-14589245	None:intergenic	45.0%
	ATGATAAGGACACCCCAGAT+AGG	+	chr1_2:14589229-14589248	Msa0058280:CDS	45.0%
	ATACACGGATAGACGAGTCA+GGG	+	chr1_2:14589492-14589511	Msa0058280:intron	45.0%
!	ACCACAAAGGTACAACCATG+AGG	+	chr1_2:14589856-14589875	Msa0058280:intron	45.0%
!	CCACAAAGGTACAACCATGA+GGG	+	chr1_2:14589857-14589876	Msa0058280:intron	45.0%
	CACGATGTATCTGTGCTGTA+TGG	+	chr1_2:14590167-14590186	Msa0058280:three_prime_UTR	45.0%
	GAATGTCCTCAAGTTCAGCT+TGG	+	chr1_2:14589163-14589182	Msa0058280:exon	45.0%
	TGTTGTCCAAGCTGAACTTG+AGG	-	chr1_2:14589172-14589191	None:intergenic	45.0%
!	TGGGGTGTCCTTATCATAGA+TGG	-	chr1_2:14589226-14589245	None:intergenic	45.0%
	ATGATAAGGACACCCCAGAT+AGG	+	chr1_2:14589229-14589248	Msa0058280:CDS	45.0%
	ATACACGGATAGACGAGTCA+GGG	+	chr1_2:14589492-14589511	Msa0058280:intron	45.0%
!	ACCACAAAGGTACAACCATG+AGG	+	chr1_2:14589856-14589875	Msa0058280:intron	45.0%
!	CCACAAAGGTACAACCATGA+GGG	+	chr1_2:14589857-14589876	Msa0058280:intron	45.0%
	CACGATGTATCTGTGCTGTA+TGG	+	chr1_2:14590167-14590186	Msa0058280:three_prime_UTR	45.0%
	ATAAGGACACCCCAGATAGG+TGG	+	chr1_2:14589232-14589251	Msa0058280:CDS	50.0%
	TAGCTTCTGCCACCTATCTG+GGG	-	chr1_2:14589244-14589263	None:intergenic	50.0%
	CTAGCTTCTGCCACCTATCT+GGG	-	chr1_2:14589245-14589264	None:intergenic	50.0%
	ATAGGTGGCAGAAGCTAGCA+AGG	+	chr1_2:14589247-14589266	Msa0058280:CDS	50.0%
	TAGGTGGCAGAAGCTAGCAA+GGG	+	chr1_2:14589248-14589267	Msa0058280:CDS	50.0%
	TGTTGGAGGGAAGACAGTTG+AGG	+	chr1_2:14589272-14589291	Msa0058280:CDS	50.0%
	CATACACGGATAGACGAGTC+AGG	+	chr1_2:14589491-14589510	Msa0058280:intron	50.0%
!	ACGAGTCAGGGTTTGAAACC+AGG	+	chr1_2:14589504-14589523	Msa0058280:intron	50.0%
	GTAAGACGAACGAAGTGACC+TGG	-	chr1_2:14589525-14589544	None:intergenic	50.0%
	CCCTCATGGTTGTACCTTTG+TGG	-	chr1_2:14589860-14589879	None:intergenic	50.0%
	CAGAACGGATCCAATATGGG+TGG	+	chr1_2:14590032-14590051	Msa0058280:three_prime_UTR	50.0%
	ATAAGGACACCCCAGATAGG+TGG	+	chr1_2:14589232-14589251	Msa0058280:CDS	50.0%
	TAGCTTCTGCCACCTATCTG+GGG	-	chr1_2:14589244-14589263	None:intergenic	50.0%
	CTAGCTTCTGCCACCTATCT+GGG	-	chr1_2:14589245-14589264	None:intergenic	50.0%
	ATAGGTGGCAGAAGCTAGCA+AGG	+	chr1_2:14589247-14589266	Msa0058280:CDS	50.0%
	TAGGTGGCAGAAGCTAGCAA+GGG	+	chr1_2:14589248-14589267	Msa0058280:CDS	50.0%
	TGTTGGAGGGAAGACAGTTG+AGG	+	chr1_2:14589272-14589291	Msa0058280:CDS	50.0%
	CATACACGGATAGACGAGTC+AGG	+	chr1_2:14589491-14589510	Msa0058280:intron	50.0%
!	ACGAGTCAGGGTTTGAAACC+AGG	+	chr1_2:14589504-14589523	Msa0058280:intron	50.0%
	GTAAGACGAACGAAGTGACC+TGG	-	chr1_2:14589525-14589544	None:intergenic	50.0%
	CCCTCATGGTTGTACCTTTG+TGG	-	chr1_2:14589860-14589879	None:intergenic	50.0%
	CAGAACGGATCCAATATGGG+TGG	+	chr1_2:14590032-14590051	Msa0058280:three_prime_UTR	50.0%
!	GCTAGCTTCTGCCACCTATC+TGG	-	chr1_2:14589246-14589265	None:intergenic	55.0%
	CAGAAGCTAGCAAGGGCTGT+TGG	+	chr1_2:14589255-14589274	Msa0058280:CDS	55.0%
	AAGCTAGCAAGGGCTGTTGG+AGG	+	chr1_2:14589258-14589277	Msa0058280:CDS	55.0%
	AGCTAGCAAGGGCTGTTGGA+GGG	+	chr1_2:14589259-14589278	Msa0058280:CDS	55.0%
!	GCTAGCTTCTGCCACCTATC+TGG	-	chr1_2:14589246-14589265	None:intergenic	55.0%
	CAGAAGCTAGCAAGGGCTGT+TGG	+	chr1_2:14589255-14589274	Msa0058280:CDS	55.0%
	AAGCTAGCAAGGGCTGTTGG+AGG	+	chr1_2:14589258-14589277	Msa0058280:CDS	55.0%
	AGCTAGCAAGGGCTGTTGGA+GGG	+	chr1_2:14589259-14589278	Msa0058280:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	14589085	14590284	14589085	ID=Msa0058280;Name=Msa0058280
chr1_2	mRNA	14589085	14590284	14589085	ID=Msa0058280-mRNA-1;Parent=Msa0058280;Name=Msa0058280-mRNA-1;_AED=0.00;_eAED=0.00;_QI=80\|1\|1\|1\|1\|1\|2\|261\|93
chr1_2	exon	14589085	14589421	14589085	ID=Msa0058280-mRNA-1:exon:5886;Parent=Msa0058280-mRNA-1
chr1_2	exon	14589999	14590284	14589999	ID=Msa0058280-mRNA-1:exon:5887;Parent=Msa0058280-mRNA-1
chr1_2	five_prime_UTR	14589085	14589164	14589085	ID=Msa0058280-mRNA-1:five_prime_utr;Parent=Msa0058280-mRNA-1
chr1_2	CDS	14589165	14589421	14589165	ID=Msa0058280-mRNA-1:cds;Parent=Msa0058280-mRNA-1
chr1_2	CDS	14589999	14590023	14589999	ID=Msa0058280-mRNA-1:cds;Parent=Msa0058280-mRNA-1
chr1_2	three_prime_UTR	14590024	14590284	14590024	ID=Msa0058280-mRNA-1:three_prime_utr;Parent=Msa0058280-mRNA-1

Gene Sequence	>Msa0058280 ATGTCCTCAAGTTCAGCTTGGACAACAAAGCAAAACAAAAGATTTGAGAATGCTTTGGCCATCTATGATAAGGACACCCCAGATAGGTGGCAGAAGCTAGCAAGGGCTGTTGGAGGGAAGACAGTTGAGGAAGTGAAAAGGCATTATGAGATGTTAGTTGAAGATTTGAAGCAAATTGAGGAAGGTCATGTTCCTTTGCCCAAATACAGAAATGCTGCAACAATTAAAGGTTACAATTACAGTGACGAAGAACAAAGGTTGAAGTATCTAAGCCTTCAGTGA
Protein sequence	>Msa0058280 MSSSSAWTTKQNKRFENALAIYDKDTPDRWQKLARAVGGKTVEEVKRHYEMLVEDLKQIEEGHVPLPKYRNAATIKGYNYSDEEQRLKYLSLQ*