AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0061170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0061170 MtrunA17_Chr1g0161111 83.983 231 21 1 1 215 60 290 1.90e-123 359
Msa0061170 MtrunA17_Chr1g0161111 92.771 83 6 0 208 290 349 431 9.80e-47 162
Msa0061170 MtrunA17_Chr1g0161081 86.047 215 14 1 17 215 17 231 5.70e-113 330
Msa0061170 MtrunA17_Chr1g0161081 96.386 83 3 0 208 290 290 372 5.14e-50 169
Msa0061170 MtrunA17_Chr1g0161041 76.623 231 31 2 1 215 31 254 4.78e-108 319
Msa0061170 MtrunA17_Chr1g0161041 87.952 83 10 0 208 290 313 395 1.46e-44 155
Msa0061170 MtrunA17_Chr7g0266651 45.327 214 96 4 21 215 8 219 2.55e-55 182
Msa0061170 MtrunA17_Chr7g0266651 51.852 81 37 1 208 286 278 358 3.15e-18 83.6
Msa0061170 MtrunA17_Chr1g0161101 61.842 152 21 3 1 152 82 196 1.63e-45 160
Msa0061170 MtrunA17_Chr1g0161101 48.734 158 22 4 152 251 346 502 7.96e-32 123
Msa0061170 MtrunA17_Chr7g0225881 38.532 218 115 2 17 215 8 225 7.35e-45 155
Msa0061170 MtrunA17_Chr7g0225881 40.000 85 47 1 208 288 284 368 1.05e-12 67.8
Msa0061170 MtrunA17_Chr1g0184241 29.745 353 160 8 21 289 18 366 6.44e-43 150
Msa0061170 MtrunA17_Chr2g0311831 29.577 355 161 10 22 289 18 370 1.38e-42 149
Msa0061170 MtrunA17_Chr6g0472861 38.053 226 116 4 14 215 5 230 1.74e-41 147
Msa0061170 MtrunA17_Chr6g0472861 44.318 88 42 3 208 290 289 374 3.04e-14 72.4
Msa0061170 MtrunA17_Chr8g0336851 27.586 348 159 8 30 289 21 363 2.22e-40 144
Msa0061170 MtrunA17_Chr8g0354241 27.978 361 164 10 21 289 17 373 1.24e-38 139
Msa0061170 MtrunA17_Chr1g0187821 38.182 220 115 3 17 215 9 228 2.66e-38 138
Msa0061170 MtrunA17_Chr1g0187821 41.667 84 46 2 208 289 287 369 6.83e-11 62.4
Msa0061170 MtrunA17_Chr8g0336631 28.775 351 156 11 28 289 19 364 7.53e-38 137
Msa0061170 MtrunA17_Chr7g0247991 36.893 206 109 3 21 207 17 220 1.04e-37 137
Msa0061170 MtrunA17_Chr2g0311761 33.796 216 123 3 22 218 18 232 7.40e-37 134
Msa0061170 MtrunA17_Chr2g0311761 40.476 84 47 2 208 289 289 371 1.23e-12 67.4
Msa0061170 MtrunA17_Chr2g0311701 34.091 220 126 2 6 206 2 221 1.34e-36 134
Msa0061170 MtrunA17_Chr8g0336751 28.571 357 164 10 21 289 43 396 2.07e-36 134
Msa0061170 MtrunA17_Chr1g0167961 35.945 217 116 4 22 218 20 233 3.68e-36 132
Msa0061170 MtrunA17_Chr2g0311711 35.922 206 113 2 17 203 11 216 4.89e-36 132
Msa0061170 MtrunA17_Chr2g0311681 35.071 211 118 2 15 206 5 215 5.98e-36 132
Msa0061170 MtrunA17_Chr1g0167911 37.073 205 107 4 22 207 5 206 8.30e-36 131
Msa0061170 MtrunA17_Chr1g0187831 36.444 225 114 5 17 215 10 231 5.22e-35 130
Msa0061170 MtrunA17_Chr1g0187831 41.379 87 45 3 208 290 290 374 1.70e-11 64.3
Msa0061170 MtrunA17_Chr2g0311691 35.071 211 118 2 15 206 5 215 5.75e-35 129
Msa0061170 MtrunA17_Chr7g0236011 33.486 218 124 4 17 215 13 228 5.88e-35 129
Msa0061170 MtrunA17_Chr1g0167951 35.193 233 119 6 22 235 5 224 7.27e-35 129
Msa0061170 MtrunA17_Chr8g0336761 33.654 208 114 3 28 215 19 222 2.28e-34 127
Msa0061170 MtrunA17_Chr8g0336761 40.476 84 47 2 208 289 281 363 3.78e-12 65.9
Msa0061170 MtrunA17_Chr1g0187811 34.211 228 124 4 17 218 125 352 2.42e-34 130
Msa0061170 MtrunA17_Chr1g0187811 43.023 86 44 3 208 289 408 492 3.67e-11 63.5
Msa0061170 MtrunA17_Chr8g0354261 33.028 218 120 4 21 215 17 231 9.37e-34 126
Msa0061170 MtrunA17_Chr8g0354261 40.000 85 47 2 208 289 290 373 1.61e-11 64.3
Msa0061170 MtrunA17_Chr8g0336861 33.493 209 114 3 28 215 19 223 1.33e-33 125
Msa0061170 MtrunA17_Chr2g0311921 32.870 216 124 4 22 218 4 217 3.62e-33 124
Msa0061170 MtrunA17_Chr8g0336781 33.014 209 115 3 28 215 54 258 4.56e-33 125
Msa0061170 MtrunA17_Chr8g0336781 40.476 84 47 2 208 289 317 399 1.01e-12 67.8
Msa0061170 MtrunA17_Chr4g0055591 35.610 205 114 2 27 215 15 217 5.15e-33 124
Msa0061170 MtrunA17_Chr8g0336831 34.826 201 106 3 28 207 19 215 1.38e-32 120
Msa0061170 MtrunA17_Chr8g0335411 33.981 206 115 3 21 207 17 220 2.77e-32 122
Msa0061170 MtrunA17_Chr7g0235951 32.766 235 128 4 21 236 17 240 3.54e-32 122
Msa0061170 MtrunA17_Chr7g0235951 38.272 81 44 2 208 283 287 366 9.61e-11 62.0
Msa0061170 MtrunA17_Chr4g0066121 31.696 224 127 4 14 215 4 223 6.56e-32 121
Msa0061170 MtrunA17_Chr4g0066991 35.149 202 114 5 21 215 17 208 1.37e-31 120
Msa0061170 MtrunA17_Chr4g0011311 32.710 214 123 3 21 215 17 228 2.59e-31 118
Msa0061170 MtrunA17_Chr8g0348081 31.731 208 118 3 28 215 19 222 3.07e-30 117
Msa0061170 MtrunA17_Chr8g0348081 39.286 84 48 2 208 289 281 363 4.18e-13 68.9
Msa0061170 MtrunA17_Chr8g0364471 29.864 221 134 3 17 218 13 231 1.07e-29 115
Msa0061170 MtrunA17_Chr1g0167931 33.500 200 105 3 22 218 5 179 1.22e-28 111
Msa0061170 MtrunA17_Chr2g0311861 31.019 216 109 4 22 218 14 208 1.60e-28 112
Msa0061170 MtrunA17_Chr2g0311861 42.857 84 45 2 208 289 264 346 9.87e-13 67.8
Msa0061170 MtrunA17_Chr2g0311741 32.086 187 108 2 48 215 2 188 9.18e-27 106
Msa0061170 MtrunA17_Chr2g0311741 33.750 80 52 1 208 287 247 325 9.77e-11 61.6
Msa0061170 MtrunA17_Chr8g0336791 33.520 179 94 3 28 185 19 193 1.27e-25 100
Msa0061170 MtrunA17_Chr1g0168001 34.320 169 87 4 22 167 5 172 2.70e-23 94.0
Msa0061170 MtrunA17_Chr8g0364161 33.537 164 88 4 21 165 17 178 3.73e-23 94.0
Msa0061170 MtrunA17_Chr8g0362741 30.698 215 111 5 21 215 17 213 7.77e-22 92.8
Msa0061170 MtrunA17_Chr7g0235991 33.803 142 75 2 74 196 22 163 6.61e-20 85.5
Msa0061170 MtrunA17_Chr7g0235981 27.358 212 105 6 21 215 3 182 1.26e-17 81.6
Msa0061170 MtrunA17_Chr8g0336841 44.578 83 43 2 209 289 9 90 2.28e-16 72.8
Msa0061170 MtrunA17_Chr2g0311641 36.937 111 65 3 35 144 20 126 8.87e-16 73.6
Msa0061170 MtrunA17_Chr3g0113641 27.136 199 121 4 24 199 2 199 2.01e-15 75.5
Msa0061170 MtrunA17_Chr7g0242551 30.000 190 105 5 37 199 15 203 2.91e-15 75.1
Msa0061170 MtrunA17_Chr1g0184111 29.442 197 110 5 43 215 9 200 3.06e-15 75.1
Msa0061170 MtrunA17_Chr1g0184121 28.426 197 112 5 43 215 9 200 3.30e-15 74.7
Msa0061170 MtrunA17_Chr6g0455481 24.658 292 129 10 73 277 42 329 5.88e-15 73.9
Msa0061170 MtrunA17_Chr2g0311631 36.937 111 65 3 35 144 20 126 2.04e-14 72.8
Msa0061170 MtrunA17_Chr8g0336801 38.095 84 49 2 208 289 73 155 4.95e-14 68.6
Msa0061170 MtrunA17_Chr1g0179801 33.913 115 72 3 36 146 26 140 1.12e-13 70.5
Msa0061170 MtrunA17_Chr2g0311621 32.479 117 74 3 18 132 2 115 1.13e-13 66.2
Msa0061170 MtrunA17_Chr7g0220001 32.215 149 79 4 73 201 41 187 1.16e-13 70.1
Msa0061170 MtrunA17_Chr2g0311751 29.457 129 72 2 58 167 12 140 1.33e-13 67.4
Msa0061170 MtrunA17_Chr5g0420891 33.913 115 71 3 37 148 12 124 5.61e-13 65.9
Msa0061170 MtrunA17_Chr1g0184151 28.977 176 98 6 43 195 10 181 6.48e-13 68.2
Msa0061170 MtrunA17_Chr5g0423211 33.051 118 68 3 37 148 13 125 1.67e-12 67.0
Msa0061170 MtrunA17_Chr5g0420901 27.568 185 113 5 37 202 12 194 3.83e-12 65.9
Msa0061170 MtrunA17_Chr5g0421091 30.435 115 75 2 37 148 11 123 5.11e-12 65.5
Msa0061170 MtrunA17_Chr5g0398291 33.333 114 68 3 37 143 8 120 5.58e-12 65.5
Msa0061170 MtrunA17_Chr8g0336871 34.821 112 62 4 182 283 57 167 6.46e-12 62.8
Msa0061170 MtrunA17_Chr5g0420941 27.027 185 114 5 37 202 11 193 8.66e-12 64.7
Msa0061170 MtrunA17_Chr3g0128001 27.273 198 117 7 40 215 5 197 1.07e-11 64.3
Msa0061170 MtrunA17_Chr1g0184161 27.841 176 98 6 43 194 10 180 1.32e-11 64.3
Msa0061170 MtrunA17_Chr4g0006371 28.141 199 106 6 37 199 14 211 2.09e-11 63.9
Msa0061170 MtrunA17_Chr5g0421101 39.506 81 47 2 209 289 269 347 2.09e-11 63.5
Msa0061170 MtrunA17_Chr3g0093751 34.615 104 62 3 35 135 16 116 5.34e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0061170 AT1G06650.2 30.303 363 159 8 19 289 8 368 2.03e-44 155
Msa0061170 AT1G06640.3 40.367 218 104 5 14 207 3 218 1.21e-43 150
Msa0061170 AT1G06640.2 40.367 218 104 5 14 207 3 218 1.62e-43 151
Msa0061170 AT1G06640.1 40.367 218 104 5 14 207 3 218 6.69e-43 150
Msa0061170 AT1G06650.1 37.557 221 112 5 19 215 8 226 5.78e-42 147
Msa0061170 AT1G03410.2 38.710 217 108 4 22 215 5 219 2.97e-41 146
Msa0061170 AT1G03410.2 40.476 84 44 2 208 287 278 359 5.69e-13 68.9
Msa0061170 AT1G03410.1 38.710 217 108 4 22 215 42 256 7.39e-41 146
Msa0061170 AT1G03410.1 40.476 84 44 2 208 287 315 396 5.06e-13 69.3
Msa0061170 AT2G30840.2 36.111 216 117 2 21 215 5 220 1.18e-40 143
Msa0061170 AT5G59540.1 28.771 358 165 9 15 283 3 359 1.56e-40 144
Msa0061170 AT2G30840.1 36.111 216 117 2 21 215 5 220 2.94e-40 144
Msa0061170 AT2G30840.3 36.111 216 117 2 21 215 5 220 3.10e-40 143
Msa0061170 AT1G06620.2 36.744 215 112 4 22 215 12 223 7.68e-39 138
Msa0061170 AT1G06620.1 36.744 215 112 4 22 215 12 223 3.97e-38 138
Msa0061170 AT1G06645.1 29.086 361 165 7 18 289 20 378 7.19e-38 137
Msa0061170 AT1G04350.1 34.906 212 121 1 21 215 6 217 7.64e-38 137
Msa0061170 AT1G04350.1 39.286 84 49 1 208 289 276 359 3.08e-12 66.6
Msa0061170 AT3G61400.2 34.361 227 120 5 14 215 5 227 1.41e-36 132
Msa0061170 AT2G30830.1 33.953 215 119 3 21 215 5 216 2.08e-36 133
Msa0061170 AT2G30830.1 38.554 83 50 1 208 289 275 357 7.14e-11 62.4
Msa0061170 AT2G25450.1 31.687 243 143 4 21 242 5 245 3.29e-36 133
Msa0061170 AT3G61400.1 34.361 227 120 5 14 215 5 227 5.90e-36 132
Msa0061170 AT3G61400.1 39.241 79 46 1 208 284 287 365 6.93e-12 65.5
Msa0061170 AT5G59530.1 36.652 221 116 4 17 215 4 222 1.16e-34 129
Msa0061170 AT5G59530.1 46.988 83 40 3 208 287 281 362 7.57e-14 71.2
Msa0061170 AT1G03400.1 33.810 210 118 2 22 215 5 209 8.10e-34 126
Msa0061170 AT1G03400.1 46.341 82 42 1 208 287 268 349 8.28e-16 77.0
Msa0061170 AT1G03400.2 33.810 210 118 2 22 215 5 209 1.85e-33 126
Msa0061170 AT1G03400.2 46.341 82 42 1 208 287 296 377 7.66e-16 77.4
Msa0061170 AT5G59540.2 31.982 222 130 3 15 215 3 224 4.08e-33 123
Msa0061170 AT5G43440.1 28.291 357 164 13 23 289 10 364 2.75e-32 122
Msa0061170 AT5G43450.2 35.000 220 123 6 15 215 2 220 2.34e-29 113
Msa0061170 AT5G43440.2 33.023 215 121 6 23 215 10 223 4.69e-29 112
Msa0061170 AT5G43450.1 35.000 220 123 6 15 215 2 220 5.66e-29 114
Msa0061170 AT1G04380.2 31.818 176 101 2 48 207 23 195 6.24e-27 106
Msa0061170 AT1G04380.1 31.818 176 101 2 48 207 23 195 1.43e-26 107
Msa0061170 AT1G04380.1 40.698 86 44 3 208 289 262 344 9.91e-12 65.1
Msa0061170 AT3G13610.1 32.381 210 115 7 27 215 16 219 1.10e-17 82.4
Msa0061170 AT3G13610.1 41.250 80 45 2 208 287 281 358 4.09e-12 66.2
Msa0061170 AT5G12270.1 34.899 149 79 6 27 173 10 142 4.33e-16 77.8
Msa0061170 AT1G55290.1 30.392 204 111 6 35 215 24 219 8.19e-16 77.0
Msa0061170 AT3G11180.2 30.526 190 104 5 37 201 56 242 1.00e-14 74.3
Msa0061170 AT3G11180.1 30.526 190 104 5 37 201 56 242 1.26e-14 73.9
Msa0061170 AT3G55970.2 26.904 197 119 6 28 199 5 201 2.56e-14 72.4
Msa0061170 AT3G55970.1 26.904 197 119 6 28 199 5 201 3.04e-14 72.4
Msa0061170 AT3G12900.1 30.476 210 110 9 35 218 20 219 1.32e-13 70.5
Msa0061170 AT5G24530.1 28.788 198 114 7 40 215 5 197 2.74e-13 69.7
Msa0061170 AT2G36690.2 31.356 118 76 2 34 146 18 135 1.48e-12 67.8
Msa0061170 AT2G36690.1 31.356 118 76 2 34 146 18 135 1.48e-12 67.8
Msa0061170 AT2G38240.1 25.000 184 118 3 37 201 12 194 1.89e-11 64.3
Msa0061170 AT5G05600.1 25.000 192 112 6 37 201 27 213 7.11e-11 62.8
Msa0061170 AT5G05600.2 25.000 192 112 6 37 201 27 213 7.77e-11 62.0
Msa0061170 AT3G19000.2 31.892 185 90 8 52 200 8 192 9.37e-11 61.6

Find 108 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGCTTATTTAAGTTATT+AGG 0.163665 1_2:+19190415 Msa0061170:three_prime_UTR
AGGTCGATTGTGGGAATTTC+TGG 0.203913 1_2:-19187365 None:intergenic
GAAACAAAATCCGGCGTAAA+AGG 0.209794 1_2:+19187248 Msa0061170:CDS
ATCTATAAAGCAGCGGTTTC+TGG 0.229034 1_2:+19190342 Msa0061170:three_prime_UTR
AGCGGCACGGGAATGGTTTA+TGG 0.269928 1_2:-19187393 None:intergenic
GGAAGTACATTGCTGAATAA+AGG 0.284600 1_2:+19190437 Msa0061170:three_prime_UTR
TCTCCCTATTACCCGGGTTT+AGG 0.289476 1_2:-19190176 None:intergenic
CATGCTTATTTAAGTTATTA+GGG 0.301172 1_2:+19190416 Msa0061170:three_prime_UTR
GTGAGGTCAGACAGAGTTTC+GGG 0.307456 1_2:-19187320 None:intergenic
TGTATATGTATGTATATATT+AGG 0.334162 1_2:+19190462 Msa0061170:three_prime_UTR
AAGATCAACATTAGATATAT+AGG 0.349799 1_2:-19187588 None:intergenic
TGAAGAAAGGCTCACAGAAT+TGG 0.350493 1_2:+19189577 Msa0061170:CDS
GAGACCCGTGGCTCGTTAGA+AGG 0.359180 1_2:-19190141 None:intergenic
GGAATCTCCTTCTCTTCCGC+TGG 0.367358 1_2:-19189458 None:intergenic
ACGGTGGTTGGTGGTGGTGA+AGG 0.371074 1_2:-19187179 None:intergenic
CGGTGGTTGGTGGTGGTGAA+GGG 0.372563 1_2:-19187178 None:intergenic
CGTTCCGCTGCAAGTACGTT+TGG 0.389362 1_2:+19187431 Msa0061170:CDS
GAAGTACATTGCTGAATAAA+GGG 0.393963 1_2:+19190438 Msa0061170:three_prime_UTR
GATTGTGGGAATTTCTGGTT+GGG 0.395562 1_2:-19187360 None:intergenic
GAAGAAAGGCTCACAGAATT+GGG 0.402379 1_2:+19189578 Msa0061170:CDS
GGTGGTGAAGGGGTGGCGAT+TGG 0.413351 1_2:-19187167 None:intergenic
GGTAATAGGGAGAAGCTCTT+TGG 0.421313 1_2:+19190186 Msa0061170:CDS
TTTCCTAAACCCGGGTAATA+GGG 0.421959 1_2:+19190173 Msa0061170:CDS
GGTGGTTGGTGGTGGTGAAG+GGG 0.423936 1_2:-19187177 None:intergenic
TCCTTCTCTTCCGCTGGGAC+AGG 0.425236 1_2:-19189452 None:intergenic
AGGAGATTCCGGAGGTATGC+AGG 0.432250 1_2:+19189471 Msa0061170:CDS
ACCAAGCCGTTACACTAATC+TGG 0.433550 1_2:+19190550 Msa0061170:three_prime_UTR
TTCTTTAAGAAAGAGCTTGA+TGG 0.440915 1_2:+19190285 Msa0061170:CDS
AGTGAGGTCAGACAGAGTTT+CGG 0.443275 1_2:-19187321 None:intergenic
GGTTGGTGGTGGTGAAGGGG+TGG 0.443343 1_2:-19187174 None:intergenic
AGCGGAAGAGAAGGAGATTC+CGG 0.446303 1_2:+19189460 Msa0061170:CDS
TGATAAATTTGTATATTATG+TGG 0.447007 1_2:-19190502 None:intergenic
TACCGTAGGGAGATGGGAAC+TGG 0.456266 1_2:+19187560 Msa0061170:CDS
CGATTGTGGGAATTTCTGGT+TGG 0.466209 1_2:-19187361 None:intergenic
TTCTCTGCTGATGTTAGCTC+TGG 0.467059 1_2:-19190216 None:intergenic
TGTTTCGTCGAAGTCTTTCA+CGG 0.467972 1_2:-19187231 None:intergenic
ACCTTCTTCCTCTCGTCCGC+AGG 0.470158 1_2:-19187536 None:intergenic
GGTGATGAGACGGTGGTTGG+TGG 0.471586 1_2:-19187188 None:intergenic
GATGAGACGGTGGTTGGTGG+TGG 0.480200 1_2:-19187185 None:intergenic
GAAGGTGTACCGTAGGGAGA+TGG 0.481378 1_2:+19187553 Msa0061170:CDS
CCGTGGCTCGTTAGAAGGAT+TGG 0.491190 1_2:-19190136 None:intergenic
TCAACTCCGGCGCGACTCCA+TGG 0.491651 1_2:-19187472 None:intergenic
ACCTCCTTCCTGCATACCTC+CGG 0.492923 1_2:-19189479 None:intergenic
ACCTGTCCCAGCGGAAGAGA+AGG 0.494521 1_2:+19189451 Msa0061170:CDS
CAGACAGAGTTTCGGGTGGA+TGG 0.495039 1_2:-19187313 None:intergenic
CTGTTCAACGACAGCGGCAC+GGG 0.497276 1_2:-19187405 None:intergenic
GTAATAGTAACTTTGTAAGA+TGG 0.504268 1_2:+19190380 Msa0061170:three_prime_UTR
GGCTTGTTGAGTGAAGGGTT+AGG 0.506041 1_2:+19189548 Msa0061170:CDS
CAGAATTGGGTTTGTCGCAA+GGG 0.514313 1_2:+19189591 Msa0061170:CDS
TTGCTGGGCTTGTTGAGTGA+AGG 0.516325 1_2:+19189542 Msa0061170:CDS
GAGTTGATGCGAAGTGTGAT+TGG 0.517223 1_2:+19187488 Msa0061170:CDS
TATGGCTGAGAGGTCGATTG+TGG 0.519394 1_2:-19187375 None:intergenic
AGAGGAAGAAGGTGTACCGT+AGG 0.520273 1_2:+19187546 Msa0061170:CDS
ACAGAATTGGGTTTGTCGCA+AGG 0.525999 1_2:+19189590 Msa0061170:CDS
TGCTGGGCTTGTTGAGTGAA+GGG 0.531596 1_2:+19189543 Msa0061170:CDS
AAGAGCTTCTCCCTATTACC+CGG 0.538989 1_2:-19190183 None:intergenic
GAATCTCCTTCTCTTCCGCT+GGG 0.540070 1_2:-19189457 None:intergenic
CAACGACAGCGGCACGGGAA+TGG 0.544177 1_2:-19187400 None:intergenic
AAGGTGTACCGTAGGGAGAT+GGG 0.544203 1_2:+19187554 Msa0061170:CDS
AAATTACCAGATTAGTGTAA+CGG 0.548742 1_2:-19190556 None:intergenic
ATGGCTGAGAGGTCGATTGT+GGG 0.548872 1_2:-19187374 None:intergenic
CGCTCAACGAATTTATGACA+AGG 0.549583 1_2:+19190262 Msa0061170:CDS
GACTTCGACGAAACAAAATC+CGG 0.555299 1_2:+19187239 Msa0061170:CDS
GTGAAATCTCTATAAAGAGC+TGG 0.556464 1_2:-19190240 None:intergenic
ACCAGATTAGTGTAACGGCT+TGG 0.558794 1_2:-19190551 None:intergenic
CAGGAAGGAGGTGATGGAGT+GGG 0.561352 1_2:+19189490 Msa0061170:CDS
ATCACACTTCGCATCAACTC+CGG 0.562598 1_2:-19187485 None:intergenic
AATTGGGTTTGTCGCAAGGG+AGG 0.563814 1_2:+19189594 Msa0061170:CDS
CCAATCCTTCTAACGAGCCA+CGG 0.564324 1_2:+19190136 Msa0061170:CDS
GGTATGCAGGAAGGAGGTGA+TGG 0.565254 1_2:+19189484 Msa0061170:CDS
CAATCCTTCTAACGAGCCAC+GGG 0.566309 1_2:+19190137 Msa0061170:CDS
ATTGTGGGAATTTCTGGTTG+GGG 0.566748 1_2:-19187359 None:intergenic
GGTTAGGATTACGTGAAGAA+AGG 0.568337 1_2:+19189564 Msa0061170:CDS
ATTGGGTTTGTCGCAAGGGA+GGG 0.570147 1_2:+19189595 Msa0061170:CDS
GGTGGATGGATGAAGAATGA+TGG 0.573720 1_2:-19187299 None:intergenic
GGTGGTGATGAGACGGTGGT+TGG 0.574489 1_2:-19187191 None:intergenic
TTCTTTCAAGTGATCAACCA+TGG 0.576207 1_2:+19187455 Msa0061170:CDS
AGAGCTTCTCCCTATTACCC+GGG 0.579455 1_2:-19190182 None:intergenic
GCTGATGTTAGCTCTGGTAA+AGG 0.581133 1_2:-19190210 None:intergenic
GAGGAAGAAGGTGTACCGTA+GGG 0.589131 1_2:+19187547 Msa0061170:CDS
CACCAGTTCCCATCTCCCTA+CGG 0.596077 1_2:-19187562 None:intergenic
ATGGATGAAGAATGATGGAA+TGG 0.596986 1_2:-19187294 None:intergenic
ATGAACAGCCTGCGGACGAG+AGG 0.602972 1_2:+19187528 Msa0061170:CDS
GCCTGCGGACGAGAGGAAGA+AGG 0.605646 1_2:+19187535 Msa0061170:CDS
GCTGTTCAACGACAGCGGCA+CGG 0.608041 1_2:-19187406 None:intergenic
GTCTCAGATAATTTCGAACG+AGG 0.609437 1_2:+19190083 Msa0061170:intron
GGATAAAGAAGTGGTGCGTG+TGG 0.614002 1_2:+19189511 Msa0061170:CDS
GCAGGAAGGAGGTGATGGAG+TGG 0.617612 1_2:+19189489 Msa0061170:CDS
TTGTGGGAATTTCTGGTTGG+GGG 0.621606 1_2:-19187358 None:intergenic
GTCGTACGGTGGTGATGAGA+CGG 0.634248 1_2:-19187198 None:intergenic
GATCAACCATGGAGTCGCGC+CGG 0.634384 1_2:+19187466 Msa0061170:CDS
GATTCCGGAGGTATGCAGGA+AGG 0.635167 1_2:+19189475 Msa0061170:CDS
GGAAATCTGATCGTGGAGTG+AGG 0.641872 1_2:-19187337 None:intergenic
GAATCCAAACGTACTTGCAG+CGG 0.644221 1_2:-19187435 None:intergenic
GATAAAGAAGTGGTGCGTGT+GGG 0.647211 1_2:+19189512 Msa0061170:CDS
TCCGGAGGTATGCAGGAAGG+AGG 0.655340 1_2:+19189478 Msa0061170:CDS
GTAAAAGGCCTAATAGACTC+CGG 0.658738 1_2:+19187263 Msa0061170:CDS
ACGAAGCTGTTCAACGACAG+CGG 0.666137 1_2:-19187411 None:intergenic
GAAGTTTCATGAACAGCCTG+CGG 0.668945 1_2:+19187520 Msa0061170:CDS
AGGTCAGACAGAGTTTCGGG+TGG 0.678541 1_2:-19187317 None:intergenic
GGAAGAGAAGGAGATTCCGG+AGG 0.687285 1_2:+19189463 Msa0061170:CDS
GGGAATGGTTTATGGCTGAG+AGG 0.688908 1_2:-19187385 None:intergenic
GATGGAGTGGGATAAAGAAG+TGG 0.690586 1_2:+19189502 Msa0061170:CDS
TAGAAGGATTGGCTAATACA+CGG 0.694993 1_2:-19190125 None:intergenic
GTGATGTATCTATAAAGCAG+CGG 0.709204 1_2:+19190335 Msa0061170:exon
AAAACAGCAATCGAGACCCG+TGG 0.709361 1_2:-19190153 None:intergenic
TAAGATGGGACCTGTCCCAG+CGG 0.729083 1_2:+19189442 Msa0061170:CDS
GTACGGTGGTGATGAGACGG+TGG 0.734372 1_2:-19187195 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTAAATTAAGATTACA+TGG + chr1_2:19188065-19188084 Msa0061170:intron 10.0%
!!! TTTTTAAATTAAGATTACAT+GGG + chr1_2:19188066-19188085 Msa0061170:intron 10.0%
!!! ACCTAATTTTTATTATATTA+GGG - chr1_2:19188268-19188287 None:intergenic 10.0%
!!! TATAGCATTTTTTTATAAGT+AGG - chr1_2:19188130-19188149 None:intergenic 15.0%
!! ACCCTAATATAATAAAAATT+AGG + chr1_2:19188264-19188283 Msa0061170:intron 15.0%
!!! GACCTAATTTTTATTATATT+AGG - chr1_2:19188269-19188288 None:intergenic 15.0%
!!! TTAAAATTAGTCATCTTTTT+AGG - chr1_2:19188495-19188514 None:intergenic 15.0%
!!! ATGAATACTTTTAAAATTAC+AGG + chr1_2:19188844-19188863 Msa0061170:intron 15.0%
!! TGTATATGTATGTATATATT+AGG + chr1_2:19190462-19190481 Msa0061170:three_prime_UTR 15.0%
!! TGATAAATTTGTATATTATG+TGG - chr1_2:19190505-19190524 None:intergenic 15.0%
!! AAGATCAACATTAGATATAT+AGG - chr1_2:19187591-19187610 None:intergenic 20.0%
!!! TTGACTAAAAAGATCATTTT+TGG - chr1_2:19188197-19188216 None:intergenic 20.0%
!!! AAAAGATGACTAATTTTAAG+TGG + chr1_2:19188496-19188515 Msa0061170:intron 20.0%
!!! AAAGATGACTAATTTTAAGT+GGG + chr1_2:19188497-19188516 Msa0061170:intron 20.0%
!!! AGTCACTAAAAGTAATTTTT+AGG - chr1_2:19188703-19188722 None:intergenic 20.0%
!! TCTAAATTAAAAAATGCAGT+TGG + chr1_2:19188883-19188902 Msa0061170:intron 20.0%
!! AAACAATTAACTAACTGAAT+CGG - chr1_2:19188981-19189000 None:intergenic 20.0%
!!! TCAGTTAGTTAATTGTTTTT+TGG + chr1_2:19188983-19189002 Msa0061170:intron 20.0%
!!! AATTTAGTTTCTAAATTTGC+AGG + chr1_2:19189231-19189250 Msa0061170:intron 20.0%
!! TTATTCAAAAAGAAAAGACA+AGG - chr1_2:19189854-19189873 None:intergenic 20.0%
!!! GAAAATTTATTGTGTTTTGA+TGG + chr1_2:19189943-19189962 Msa0061170:intron 20.0%
!! TGAGAAATCCAAATTAAAAA+AGG - chr1_2:19190049-19190068 None:intergenic 20.0%
!!! ATTATCTGAGACAAAAATTA+TGG - chr1_2:19190075-19190094 None:intergenic 20.0%
!!! TCATGCTTATTTAAGTTATT+AGG + chr1_2:19190415-19190434 Msa0061170:three_prime_UTR 20.0%
!!! CATGCTTATTTAAGTTATTA+GGG + chr1_2:19190416-19190435 Msa0061170:three_prime_UTR 20.0%
!! TCATAAATAATTTTCGAACC+TGG - chr1_2:19187649-19187668 None:intergenic 25.0%
! AATATATCACAAACACATTC+AGG + chr1_2:19187711-19187730 Msa0061170:intron 25.0%
! AACTTATAACAGATAGTTGA+TGG + chr1_2:19187832-19187851 Msa0061170:intron 25.0%
! TTATAACAAATAGCTGTTAG+TGG + chr1_2:19187939-19187958 Msa0061170:intron 25.0%
!! AAAACTAAAAGGACTTGTTT+GGG - chr1_2:19188610-19188629 None:intergenic 25.0%
!! AAAAACTAAAAGGACTTGTT+TGG - chr1_2:19188609-19188628 None:intergenic 25.0%
! GAAACGATTCAAAAACTAAA+AGG - chr1_2:19188599-19188618 None:intergenic 25.0%
!!! TTTCAAACAAGTCATTTTAG+AGG + chr1_2:19188413-19188432 Msa0061170:intron 25.0%
!!! ATCTTTTTAGGTATGTCAAA+TGG - chr1_2:19188483-19188502 None:intergenic 25.0%
! GAAACGATTCAAAAACTAAA+AGG + chr1_2:19188599-19188618 Msa0061170:intron 25.0%
!! AAAAACTAAAAGGACTTGTT+TGG + chr1_2:19188609-19188628 Msa0061170:intron 25.0%
!! AAAACTAAAAGGACTTGTTT+GGG + chr1_2:19188610-19188629 Msa0061170:intron 25.0%
!!! CATATTTTTTCACTTTCTCA+TGG - chr1_2:19188790-19188809 None:intergenic 25.0%
! AGTAACATAATCAAACGTAT+CGG - chr1_2:19189140-19189159 None:intergenic 25.0%
! ATAAAAGACTGATGAAATTG+AGG - chr1_2:19189285-19189304 None:intergenic 25.0%
!! TACAATTTTCAGAACCAAAA+TGG + chr1_2:19189385-19189404 Msa0061170:intron 25.0%
!! ATAACGATGAAATACCATTT+TGG - chr1_2:19189402-19189421 None:intergenic 25.0%
!! GTATTTCATCGTTATTTCAA+TGG + chr1_2:19189407-19189426 Msa0061170:intron 25.0%
!! ATGTTCACATCAATTTTTCA+AGG + chr1_2:19189914-19189933 Msa0061170:intron 25.0%
!!! TTTGCTGTCCTTTTTTAATT+TGG + chr1_2:19190038-19190057 Msa0061170:intron 25.0%
! GTAATAGTAACTTTGTAAGA+TGG + chr1_2:19190380-19190399 Msa0061170:three_prime_UTR 25.0%
!! TTATAACAGATAGTTGATGG+TGG + chr1_2:19187835-19187854 Msa0061170:intron 30.0%
! ATAAGCTTGTGAAAAAGTGT+TGG + chr1_2:19188168-19188187 Msa0061170:intron 30.0%
ATTAGGTCAAATGCATCTAA+AGG + chr1_2:19188281-19188300 Msa0061170:intron 30.0%
GGGAAAAACGAAAAACTTAA+AGG - chr1_2:19188659-19188678 None:intergenic 30.0%
GAAACTTAAAGGATCACTTT+GGG - chr1_2:19188328-19188347 None:intergenic 30.0%
TGAAACTTAAAGGATCACTT+TGG - chr1_2:19188329-19188348 None:intergenic 30.0%
!!! TTGGGACTTTTGAAACTTAA+AGG - chr1_2:19188628-19188647 None:intergenic 30.0%
! GAAACTTAAAGGACTTGTTT+GGG - chr1_2:19188357-19188376 None:intergenic 30.0%
! TGAAACTTAAAGGACTTGTT+TGG - chr1_2:19188358-19188377 None:intergenic 30.0%
!!! TTGGGACTTTTGAAACTTAA+AGG - chr1_2:19188628-19188647 None:intergenic 30.0%
!!! TTTTTAGGTATGTCAAATGG+TGG - chr1_2:19188480-19188499 None:intergenic 30.0%
!! CCTATTTTAGATAGCATAGT+TGG - chr1_2:19188571-19188590 None:intergenic 30.0%
! CCAACTATGCTATCTAAAAT+AGG + chr1_2:19188568-19188587 Msa0061170:intron 30.0%
!!! TTGGGACTTTTGAAACTTAA+AGG + chr1_2:19188628-19188647 Msa0061170:intron 30.0%
GGGAAAAACGAAAAACTTAA+AGG + chr1_2:19188659-19188678 Msa0061170:intron 30.0%
TTTAGGTCAAATGCATCTAA+AGG - chr1_2:19188686-19188705 None:intergenic 30.0%
ATGTTACTCACACACTAAAT+AGG + chr1_2:19189152-19189171 Msa0061170:intron 30.0%
ATTAACATGATCAACCCAAT+GGG + chr1_2:19189318-19189337 Msa0061170:intron 30.0%
! TGGAATTTTGCAGATTAAGA+TGG + chr1_2:19189427-19189446 Msa0061170:intron 30.0%
! GGAATTTTGCAGATTAAGAT+GGG + chr1_2:19189428-19189447 Msa0061170:intron 30.0%
CAACATTGGAGATTTATTTC+AGG + chr1_2:19189793-19189812 Msa0061170:intron 30.0%
CACAATCACAAAACACTAAT+TGG - chr1_2:19189987-19190006 None:intergenic 30.0%
! TTCTTTAAGAAAGAGCTTGA+TGG + chr1_2:19190285-19190304 Msa0061170:CDS 30.0%
GAAGTACATTGCTGAATAAA+GGG + chr1_2:19190438-19190457 Msa0061170:three_prime_UTR 30.0%
!! TTTTGATTCCGGAGTCTATT+AGG - chr1_2:19187274-19187293 None:intergenic 35.0%
!!! TGATGGAATGGTTTTGATTC+CGG - chr1_2:19187285-19187304 None:intergenic 35.0%
! ATGGATGAAGAATGATGGAA+TGG - chr1_2:19187297-19187316 None:intergenic 35.0%
! TTCTTTCAAGTGATCAACCA+TGG + chr1_2:19187455-19187474 Msa0061170:CDS 35.0%
ACCAAAGAAAAAACACGACT+AGG + chr1_2:19187768-19187787 Msa0061170:intron 35.0%
!!! TCCTAGTCGTGTTTTTTCTT+TGG - chr1_2:19187772-19187791 None:intergenic 35.0%
GTTGAAAAACCAGCTTATAG+AGG + chr1_2:19187910-19187929 Msa0061170:intron 35.0%
TAAGTTCATCCTCTATAAGC+TGG - chr1_2:19187922-19187941 None:intergenic 35.0%
GTAGTGTTCAGTAGAACTAA+TGG + chr1_2:19187983-19188002 Msa0061170:intron 35.0%
!! CAAGTCATTTTAGAGGATGA+TGG + chr1_2:19188420-19188439 Msa0061170:intron 35.0%
!! ACTAATTTTAAGTGGGTGTC+AGG + chr1_2:19188504-19188523 Msa0061170:intron 35.0%
TGAAACTTAAAGGACCACTT+TGG + chr1_2:19188638-19188657 Msa0061170:intron 35.0%
GAAACTTAAAGGACCACTTT+GGG + chr1_2:19188639-19188658 Msa0061170:intron 35.0%
!! GTTTTTCGTTTTTCCCAAAG+TGG - chr1_2:19188312-19188331 None:intergenic 35.0%
TAATCAAACGTATCGGAAGA+AGG - chr1_2:19189133-19189152 None:intergenic 35.0%
GATTAACATGATCAACCCAA+TGG + chr1_2:19189317-19189336 Msa0061170:intron 35.0%
GTATTGTTGCAGGATCATAT+TGG + chr1_2:19189704-19189723 Msa0061170:intron 35.0%
GACATTAATCCAACCATGTT+GGG - chr1_2:19189747-19189766 None:intergenic 35.0%
TGACATTAATCCAACCATGT+TGG - chr1_2:19189748-19189767 None:intergenic 35.0%
ATTAATGTCAAGCCTCTTGA+TGG + chr1_2:19189758-19189777 Msa0061170:intron 35.0%
!!! TGTGTTTTGATGGAAATCAC+AGG + chr1_2:19189953-19189972 Msa0061170:intron 35.0%
!!! GTGTTTTGATGGAAATCACA+GGG + chr1_2:19189954-19189973 Msa0061170:intron 35.0%
CTTGGCATTACGAAAAGAAA+AGG - chr1_2:19190015-19190034 None:intergenic 35.0%
AAAAAGGACAGCAAAATGCT+TGG - chr1_2:19190033-19190052 None:intergenic 35.0%
!! TAGAAGGATTGGCTAATACA+CGG - chr1_2:19190128-19190147 None:intergenic 35.0%
GTGAAATCTCTATAAAGAGC+TGG - chr1_2:19190243-19190262 None:intergenic 35.0%
GTGATGTATCTATAAAGCAG+CGG + chr1_2:19190335-19190354 Msa0061170:exon 35.0%
! GGAAGTACATTGCTGAATAA+AGG + chr1_2:19190437-19190456 Msa0061170:three_prime_UTR 35.0%
! AGTCTTTCACGGCTTTTACA+CGG - chr1_2:19187223-19187242 None:intergenic 40.0%
TGTTTCGTCGAAGTCTTTCA+CGG - chr1_2:19187234-19187253 None:intergenic 40.0%
GACTTCGACGAAACAAAATC+CGG + chr1_2:19187239-19187258 Msa0061170:CDS 40.0%
GAAACAAAATCCGGCGTAAA+AGG + chr1_2:19187248-19187267 Msa0061170:CDS 40.0%
GTAAAAGGCCTAATAGACTC+CGG + chr1_2:19187263-19187282 Msa0061170:CDS 40.0%
!! ATTGTGGGAATTTCTGGTTG+GGG - chr1_2:19187362-19187381 None:intergenic 40.0%
!! GATTGTGGGAATTTCTGGTT+GGG - chr1_2:19187363-19187382 None:intergenic 40.0%
CTCATCTTTGGTACCAAATC+TGG - chr1_2:19188532-19188551 None:intergenic 40.0%
TGGTACCAAAGATGAGTTCA+AGG + chr1_2:19188536-19188555 Msa0061170:intron 40.0%
TGCTTCCTTGAACTCATCTT+TGG - chr1_2:19188544-19188563 None:intergenic 40.0%
!! CTGAATCGGTTCGTAACAAT+CGG - chr1_2:19188967-19188986 None:intergenic 40.0%
GGTTAGGATTACGTGAAGAA+AGG + chr1_2:19189564-19189583 Msa0061170:CDS 40.0%
TGAAGAAAGGCTCACAGAAT+TGG + chr1_2:19189577-19189596 Msa0061170:CDS 40.0%
GAAGAAAGGCTCACAGAATT+GGG + chr1_2:19189578-19189597 Msa0061170:CDS 40.0%
TTGAGTCCAACGGTAAGATT+AGG - chr1_2:19189656-19189675 None:intergenic 40.0%
TGCATGAGAATTGAGTCCAA+CGG - chr1_2:19189666-19189685 None:intergenic 40.0%
TCAATTCTCATGCAGATCCT+GGG + chr1_2:19189672-19189691 Msa0061170:intron 40.0%
TTGTTGCAGGATCATATTGG+TGG + chr1_2:19189707-19189726 Msa0061170:intron 40.0%
! ATATTGGTGGCTTACAGGTT+AGG + chr1_2:19189720-19189739 Msa0061170:intron 40.0%
ATAACCAAAGCTCCATCAAG+AGG - chr1_2:19189773-19189792 None:intergenic 40.0%
! GGAGCTTTGGTTATCAACAT+TGG + chr1_2:19189779-19189798 Msa0061170:intron 40.0%
GTCTCAGATAATTTCGAACG+AGG + chr1_2:19190083-19190102 Msa0061170:intron 40.0%
!!! GATTGCTGTTTTCCTAAACC+CGG + chr1_2:19190164-19190183 Msa0061170:CDS 40.0%
!!! ATTGCTGTTTTCCTAAACCC+GGG + chr1_2:19190165-19190184 Msa0061170:CDS 40.0%
! TTTTCCTAAACCCGGGTAAT+AGG + chr1_2:19190172-19190191 Msa0061170:CDS 40.0%
TTTCCTAAACCCGGGTAATA+GGG + chr1_2:19190173-19190192 Msa0061170:CDS 40.0%
CGCTCAACGAATTTATGACA+AGG + chr1_2:19190262-19190281 Msa0061170:CDS 40.0%
ATCTATAAAGCAGCGGTTTC+TGG + chr1_2:19190342-19190361 Msa0061170:three_prime_UTR 40.0%
! GTCTATTAGGCCTTTTACGC+CGG - chr1_2:19187261-19187280 None:intergenic 45.0%
GGTGGATGGATGAAGAATGA+TGG - chr1_2:19187302-19187321 None:intergenic 45.0%
AGTGAGGTCAGACAGAGTTT+CGG - chr1_2:19187324-19187343 None:intergenic 45.0%
!! TTGTGGGAATTTCTGGTTGG+GGG - chr1_2:19187361-19187380 None:intergenic 45.0%
!! CGATTGTGGGAATTTCTGGT+TGG - chr1_2:19187364-19187383 None:intergenic 45.0%
AGGTCGATTGTGGGAATTTC+TGG - chr1_2:19187368-19187387 None:intergenic 45.0%
GAATCCAAACGTACTTGCAG+CGG - chr1_2:19187438-19187457 None:intergenic 45.0%
ATCACACTTCGCATCAACTC+CGG - chr1_2:19187488-19187507 None:intergenic 45.0%
!! GAGTTGATGCGAAGTGTGAT+TGG + chr1_2:19187488-19187507 Msa0061170:CDS 45.0%
GAAGTTTCATGAACAGCCTG+CGG + chr1_2:19187520-19187539 Msa0061170:CDS 45.0%
TCGAGCACATTAGTCCCATT+GGG - chr1_2:19189335-19189354 None:intergenic 45.0%
GATGGAGTGGGATAAAGAAG+TGG + chr1_2:19189502-19189521 Msa0061170:CDS 45.0%
GATAAAGAAGTGGTGCGTGT+GGG + chr1_2:19189512-19189531 Msa0061170:CDS 45.0%
! CGTGTGGGAGATATTTTGCT+GGG + chr1_2:19189527-19189546 Msa0061170:CDS 45.0%
ACAGAATTGGGTTTGTCGCA+AGG + chr1_2:19189590-19189609 Msa0061170:CDS 45.0%
CAGAATTGGGTTTGTCGCAA+GGG + chr1_2:19189591-19189610 Msa0061170:CDS 45.0%
GAGGGCAAAATGGGTAATAG+TGG - chr1_2:19189631-19189650 None:intergenic 45.0%
GATTAGGCTGAGGGCAAAAT+GGG - chr1_2:19189640-19189659 None:intergenic 45.0%
AGATTAGGCTGAGGGCAAAA+TGG - chr1_2:19189641-19189660 None:intergenic 45.0%
CAACGGTAAGATTAGGCTGA+GGG - chr1_2:19189649-19189668 None:intergenic 45.0%
CTCAATTCTCATGCAGATCC+TGG + chr1_2:19189671-19189690 Msa0061170:intron 45.0%
CAATTCTCATGCAGATCCTG+GGG + chr1_2:19189673-19189692 Msa0061170:intron 45.0%
AACAATACTGTCAATGCCCC+AGG - chr1_2:19189692-19189711 None:intergenic 45.0%
!! GGCATTGACAGTATTGTTGC+AGG + chr1_2:19189694-19189713 Msa0061170:intron 45.0%
! GGATCATATTGGTGGCTTAC+AGG + chr1_2:19189715-19189734 Msa0061170:intron 45.0%
TTACAGGTTAGGTCCCAACA+TGG + chr1_2:19189731-19189750 Msa0061170:intron 45.0%
AAGAGCTTCTCCCTATTACC+CGG - chr1_2:19190186-19190205 None:intergenic 45.0%
GGTAATAGGGAGAAGCTCTT+TGG + chr1_2:19190186-19190205 Msa0061170:CDS 45.0%
!! GCTGATGTTAGCTCTGGTAA+AGG - chr1_2:19190213-19190232 None:intergenic 45.0%
TTCTCTGCTGATGTTAGCTC+TGG - chr1_2:19190219-19190238 None:intergenic 45.0%
! ACGGCTTTTACACGGTCGTA+CGG - chr1_2:19187215-19187234 None:intergenic 50.0%
GTGAGGTCAGACAGAGTTTC+GGG - chr1_2:19187323-19187342 None:intergenic 50.0%
GGAAATCTGATCGTGGAGTG+AGG - chr1_2:19187340-19187359 None:intergenic 50.0%
ATGGCTGAGAGGTCGATTGT+GGG - chr1_2:19187377-19187396 None:intergenic 50.0%
TATGGCTGAGAGGTCGATTG+TGG - chr1_2:19187378-19187397 None:intergenic 50.0%
GGGAATGGTTTATGGCTGAG+AGG - chr1_2:19187388-19187407 None:intergenic 50.0%
ACGAAGCTGTTCAACGACAG+CGG - chr1_2:19187414-19187433 None:intergenic 50.0%
! AGAGGAAGAAGGTGTACCGT+AGG + chr1_2:19187546-19187565 Msa0061170:CDS 50.0%
! GAGGAAGAAGGTGTACCGTA+GGG + chr1_2:19187547-19187566 Msa0061170:CDS 50.0%
AAGGTGTACCGTAGGGAGAT+GGG + chr1_2:19187554-19187573 Msa0061170:CDS 50.0%
GTCGAGCACATTAGTCCCAT+TGG - chr1_2:19189336-19189355 None:intergenic 50.0%
GAATCTCCTTCTCTTCCGCT+GGG - chr1_2:19189460-19189479 None:intergenic 50.0%
AGCGGAAGAGAAGGAGATTC+CGG + chr1_2:19189460-19189479 Msa0061170:CDS 50.0%
GGATAAAGAAGTGGTGCGTG+TGG + chr1_2:19189511-19189530 Msa0061170:CDS 50.0%
! GCGTGTGGGAGATATTTTGC+TGG + chr1_2:19189526-19189545 Msa0061170:CDS 50.0%
!! TTGCTGGGCTTGTTGAGTGA+AGG + chr1_2:19189542-19189561 Msa0061170:CDS 50.0%
!! TGCTGGGCTTGTTGAGTGAA+GGG + chr1_2:19189543-19189562 Msa0061170:CDS 50.0%
!! GGCTTGTTGAGTGAAGGGTT+AGG + chr1_2:19189548-19189567 Msa0061170:CDS 50.0%
AATTGGGTTTGTCGCAAGGG+AGG + chr1_2:19189594-19189613 Msa0061170:CDS 50.0%
ATTGGGTTTGTCGCAAGGGA+GGG + chr1_2:19189595-19189614 Msa0061170:CDS 50.0%
CCAACGGTAAGATTAGGCTG+AGG - chr1_2:19189650-19189669 None:intergenic 50.0%
CCTCAGCCTAATCTTACCGT+TGG + chr1_2:19189647-19189666 Msa0061170:intron 50.0%
AGGTTAGGTCCCAACATGGT+TGG + chr1_2:19189735-19189754 Msa0061170:intron 50.0%
CAAGCCTCTTGATGGAGCTT+TGG + chr1_2:19189766-19189785 Msa0061170:intron 50.0%
CCAATCCTTCTAACGAGCCA+CGG + chr1_2:19190136-19190155 Msa0061170:CDS 50.0%
CAATCCTTCTAACGAGCCAC+GGG + chr1_2:19190137-19190156 Msa0061170:CDS 50.0%
AAAACAGCAATCGAGACCCG+TGG - chr1_2:19190156-19190175 None:intergenic 50.0%
TCTCCCTATTACCCGGGTTT+AGG - chr1_2:19190179-19190198 None:intergenic 50.0%
AGAGCTTCTCCCTATTACCC+GGG - chr1_2:19190185-19190204 None:intergenic 50.0%
! GTCGTACGGTGGTGATGAGA+CGG - chr1_2:19187201-19187220 None:intergenic 55.0%
! GCTTTTACACGGTCGTACGG+TGG - chr1_2:19187212-19187231 None:intergenic 55.0%
! CAGACAGAGTTTCGGGTGGA+TGG - chr1_2:19187316-19187335 None:intergenic 55.0%
AGGTCAGACAGAGTTTCGGG+TGG - chr1_2:19187320-19187339 None:intergenic 55.0%
GGTTGGGGGAAATCTGATCG+TGG - chr1_2:19187347-19187366 None:intergenic 55.0%
AGCGGCACGGGAATGGTTTA+TGG - chr1_2:19187396-19187415 None:intergenic 55.0%
CGTTCCGCTGCAAGTACGTT+TGG + chr1_2:19187431-19187450 Msa0061170:CDS 55.0%
! GAAGGTGTACCGTAGGGAGA+TGG + chr1_2:19187553-19187572 Msa0061170:CDS 55.0%
TACCGTAGGGAGATGGGAAC+TGG + chr1_2:19187560-19187579 Msa0061170:CDS 55.0%
CACCAGTTCCCATCTCCCTA+CGG - chr1_2:19187565-19187584 None:intergenic 55.0%
!! TCGCTTCTAAAGCTGCCAGC+TGG + chr1_2:19187612-19187631 Msa0061170:intron 55.0%
TGGGTGTCAGGAGCCAGATT+TGG + chr1_2:19188516-19188535 Msa0061170:intron 55.0%
TAAGATGGGACCTGTCCCAG+CGG + chr1_2:19189442-19189461 Msa0061170:CDS 55.0%
GGAATCTCCTTCTCTTCCGC+TGG - chr1_2:19189461-19189480 None:intergenic 55.0%
GGAAGAGAAGGAGATTCCGG+AGG + chr1_2:19189463-19189482 Msa0061170:CDS 55.0%
! AGGAGATTCCGGAGGTATGC+AGG + chr1_2:19189471-19189490 Msa0061170:CDS 55.0%
! GATTCCGGAGGTATGCAGGA+AGG + chr1_2:19189475-19189494 Msa0061170:CDS 55.0%
ACCTCCTTCCTGCATACCTC+CGG - chr1_2:19189482-19189501 None:intergenic 55.0%
GGTATGCAGGAAGGAGGTGA+TGG + chr1_2:19189484-19189503 Msa0061170:CDS 55.0%
!! CAGGAAGGAGGTGATGGAGT+GGG + chr1_2:19189490-19189509 Msa0061170:CDS 55.0%
TTTGTCGCAAGGGAGGGTGA+TGG + chr1_2:19189601-19189620 Msa0061170:intron 55.0%
CCGTGGCTCGTTAGAAGGAT+TGG - chr1_2:19190139-19190158 None:intergenic 55.0%
!! GGTGGTTGGTGGTGGTGAAG+GGG - chr1_2:19187180-19187199 None:intergenic 60.0%
!! CGGTGGTTGGTGGTGGTGAA+GGG - chr1_2:19187181-19187200 None:intergenic 60.0%
!! ACGGTGGTTGGTGGTGGTGA+AGG - chr1_2:19187182-19187201 None:intergenic 60.0%
!! GATGAGACGGTGGTTGGTGG+TGG - chr1_2:19187188-19187207 None:intergenic 60.0%
GGTGATGAGACGGTGGTTGG+TGG - chr1_2:19187191-19187210 None:intergenic 60.0%
! GGTGGTGATGAGACGGTGGT+TGG - chr1_2:19187194-19187213 None:intergenic 60.0%
! GTACGGTGGTGATGAGACGG+TGG - chr1_2:19187198-19187217 None:intergenic 60.0%
CTGTTCAACGACAGCGGCAC+GGG - chr1_2:19187408-19187427 None:intergenic 60.0%
GCTGTTCAACGACAGCGGCA+CGG - chr1_2:19187409-19187428 None:intergenic 60.0%
GATCAACCATGGAGTCGCGC+CGG + chr1_2:19187466-19187485 Msa0061170:CDS 60.0%
ATGAACAGCCTGCGGACGAG+AGG + chr1_2:19187528-19187547 Msa0061170:CDS 60.0%
ACCTTCTTCCTCTCGTCCGC+AGG - chr1_2:19187539-19187558 None:intergenic 60.0%
ACCTGTCCCAGCGGAAGAGA+AGG + chr1_2:19189451-19189470 Msa0061170:CDS 60.0%
TCCTTCTCTTCCGCTGGGAC+AGG - chr1_2:19189455-19189474 None:intergenic 60.0%
! TCCGGAGGTATGCAGGAAGG+AGG + chr1_2:19189478-19189497 Msa0061170:CDS 60.0%
!! GCAGGAAGGAGGTGATGGAG+TGG + chr1_2:19189489-19189508 Msa0061170:CDS 60.0%
! TCGCAAGGGAGGGTGATGGT+TGG + chr1_2:19189605-19189624 Msa0061170:intron 60.0%
GAGACCCGTGGCTCGTTAGA+AGG - chr1_2:19190144-19190163 None:intergenic 60.0%
GGTGGTGAAGGGGTGGCGAT+TGG - chr1_2:19187170-19187189 None:intergenic 65.0%
!! GGTTGGTGGTGGTGAAGGGG+TGG - chr1_2:19187177-19187196 None:intergenic 65.0%
CAACGACAGCGGCACGGGAA+TGG - chr1_2:19187403-19187422 None:intergenic 65.0%
TCAACTCCGGCGCGACTCCA+TGG - chr1_2:19187475-19187494 None:intergenic 65.0%
GCCTGCGGACGAGAGGAAGA+AGG + chr1_2:19187535-19187554 Msa0061170:CDS 65.0%
CTGGAGTGTGTCACGCCAGC+TGG - chr1_2:19187630-19187649 None:intergenic 65.0%
CAGCTGGCGTGACACACTCC+AGG + chr1_2:19187628-19187647 Msa0061170:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 19187155 19190570 19187155 ID=Msa0061170;Name=Msa0061170
chr1_2 mRNA 19187155 19190570 19187155 ID=Msa0061170-mRNA-1;Parent=Msa0061170;Name=Msa0061170-mRNA-1;_AED=0.18;_eAED=0.21;_QI=0|0|0.33|1|1|1|3|232|290
chr1_2 exon 19187155 19187601 19187155 ID=Msa0061170-mRNA-1:exon:7475;Parent=Msa0061170-mRNA-1
chr1_2 exon 19189440 19189616 19189440 ID=Msa0061170-mRNA-1:exon:7476;Parent=Msa0061170-mRNA-1
chr1_2 exon 19190090 19190570 19190090 ID=Msa0061170-mRNA-1:exon:7477;Parent=Msa0061170-mRNA-1
chr1_2 CDS 19187155 19187601 19187155 ID=Msa0061170-mRNA-1:cds;Parent=Msa0061170-mRNA-1
chr1_2 CDS 19189440 19189616 19189440 ID=Msa0061170-mRNA-1:cds;Parent=Msa0061170-mRNA-1
chr1_2 CDS 19190090 19190338 19190090 ID=Msa0061170-mRNA-1:cds;Parent=Msa0061170-mRNA-1
chr1_2 three_prime_UTR 19190339 19190570 19190339 ID=Msa0061170-mRNA-1:three_prime_utr;Parent=Msa0061170-mRNA-1
Gene Sequence

>Msa0061170

ATGTCATCATCACCAATCGCCACCCCTTCACCACCACCAACCACCGTCTCATCACCACCGTACGACCGTGTAAAAGCCGTGAAAGACTTCGACGAAACAAAATCCGGCGTAAAAGGCCTAATAGACTCCGGAATCAAAACCATTCCATCATTCTTCATCCATCCACCCGAAACTCTGTCTGACCTCACTCCACGATCAGATTTCCCCCAACCAGAAATTCCCACAATCGACCTCTCAGCCATAAACCATTCCCGTGCCGCTGTCGTTGAACAGCTTCGTTCCGCTGCAAGTACGTTTGGATTCTTTCAAGTGATCAACCATGGAGTCGCGCCGGAGTTGATGCGAAGTGTGATTGGTGCTATGAAGAAGTTTCATGAACAGCCTGCGGACGAGAGGAAGAAGGTGTACCGTAGGGAGATGGGAACTGGTGTTTCCTATATATCTAATATTAAGATGGGACCTGTCCCAGCGGAAGAGAAGGAGATTCCGGAGGTATGCAGGAAGGAGGTGATGGAGTGGGATAAAGAAGTGGTGCGTGTGGGAGATATTTTGCTGGGCTTGTTGAGTGAAGGGTTAGGATTACGTGAAGAAAGGCTCACAGAATTGGGTTTGTCGCAAGGGAGGATAATTTCGAACGAGGAGTATAAGAGTGCTGATCACCGTGTATTAGCCAATCCTTCTAACGAGCCACGGGTCTCGATTGCTGTTTTCCTAAACCCGGGTAATAGGGAGAAGCTCTTTGGTCCTTTACCAGAGCTAACATCAGCAGAGAAGCCAGCTCTTTATAGAGATTTCACGCTCAACGAATTTATGACAAGGTTCTTTAAGAAAGAGCTTGATGGTAAATCATTGACTAATTTCTTCAGAAAGTGA
Protein sequence

>Msa0061170

MSSSPIATPSPPPTTVSSPPYDRVKAVKDFDETKSGVKGLIDSGIKTIPSFFIHPPETLSDLTPRSDFPQPEIPTIDLSAINHSRAAVVEQLRSAASTFGFFQVINHGVAPELMRSVIGAMKKFHEQPADERKKVYRREMGTGVSYISNIKMGPVPAEEKEIPEVCRKEVMEWDKEVVRVGDILLGLLSEGLGLREERLTELGLSQGRIISNEEYKSADHRVLANPSNEPRVSIAVFLNPGNREKLFGPLPELTSAEKPALYRDFTLNEFMTRFFKKELDGKSLTNFFRK*