AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0065930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0065930 MtrunA17_Chr1g0169691 99.286 140 1 0 1 140 189 328 2.20e-97 283
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0065930 AT1G04690.1 80.714 140 27 0 1 140 189 328 1.98e-79 238

Find 57 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTGCTTGAATTGACTTAT+TGG 0.146959 1_2:+28785794 Msa0065930:three_prime_UTR
GATTCGTACAGATGAATTTA+AGG 0.191920 1_2:+28785434 Msa0065930:exon
CTTGCGTCTGGAGTCCTTAC+TGG 0.288666 1_2:+28783207 Msa0065930:CDS
CATTTGGAATACTTAATAAA+AGG 0.292147 1_2:-28785570 None:intergenic
CCTAGATTAATATATGCTTA+TGG 0.297538 1_2:+28785654 Msa0065930:three_prime_UTR
CTTCCCCTGTATTCTAGTTA+TGG 0.322795 1_2:+28783159 Msa0065930:CDS
TGGATTTAAGACTGGGATTT+GGG 0.323998 1_2:+28785616 Msa0065930:three_prime_UTR
ATGGATTTAAGACTGGGATT+TGG 0.325092 1_2:+28785615 Msa0065930:three_prime_UTR
TCCAAAGCAAAACGGCTGTC+TGG 0.347444 1_2:-28783255 None:intergenic
TAAAATATCAGGGTATTGTT+TGG 0.347641 1_2:+28785698 Msa0065930:three_prime_UTR
ACGCCCAGTTCATCTGCTAT+TGG 0.347932 1_2:-28783903 None:intergenic
TGTTCACGGTAATAACTTCA+TGG 0.384375 1_2:-28785757 None:intergenic
CTGTACGAATCAGCGCGCTT+TGG 0.385768 1_2:-28785422 None:intergenic
CAATGCTCCTAAAACACATT+TGG 0.387127 1_2:-28785586 None:intergenic
CCAAAGCAAAACGGCTGTCT+GGG 0.403649 1_2:-28783254 None:intergenic
CCATAAGCATATATTAATCT+AGG 0.404654 1_2:-28785654 None:intergenic
CTGTATTCTAGTTATGGAAT+TGG 0.406038 1_2:+28783165 Msa0065930:CDS
GAAGCCAATAGCAGATGAAC+TGG 0.416791 1_2:+28783899 Msa0065930:CDS
GTTTCATGATCCCGTTTGTT+GGG 0.418001 1_2:+28785499 Msa0065930:three_prime_UTR
GCAATTGAAAGCTGTGCTAA+TGG 0.419930 1_2:-28783927 None:intergenic
CAACATATATGTGCATTCAC+TGG 0.422871 1_2:+28785473 Msa0065930:three_prime_UTR
ATCAAGTGAAACGAAACAAT+TGG 0.434217 1_2:-28785734 None:intergenic
GGTTTCATGATCCCGTTTGT+TGG 0.438584 1_2:+28785498 Msa0065930:three_prime_UTR
CAGATAACCGACGAAACATT+AGG 0.438645 1_2:-28783966 None:intergenic
GGTTGATGATGTGCTCAAAA+AGG 0.442188 1_2:+28783866 Msa0065930:CDS
ACTTGGAGTCCACTTGCGTC+TGG 0.442488 1_2:+28783195 Msa0065930:CDS
CAATTCCATAACTAGAATAC+AGG 0.449939 1_2:-28783164 None:intergenic
GTACTCCATGGATTTAAGAC+TGG 0.466654 1_2:+28785608 Msa0065930:three_prime_UTR
AAAGTTGCACTCCCAACAAA+CGG 0.469442 1_2:-28785510 None:intergenic
TACTCCATGGATTTAAGACT+GGG 0.480613 1_2:+28785609 Msa0065930:three_prime_UTR
TGGCGCTACAAAAGAGTCTC+AGG 0.481315 1_2:+28783989 Msa0065930:CDS
ACTTAATCACGTCTTGTTCA+CGG 0.492216 1_2:-28785771 None:intergenic
TTAGATTCATGAGAATATGA+AGG 0.510165 1_2:+28785331 Msa0065930:intron
AAGTTGCACTCCCAACAAAC+GGG 0.513609 1_2:-28785509 None:intergenic
TTTAGGAGCATTGTACTCCA+TGG 0.514260 1_2:+28785596 Msa0065930:three_prime_UTR
GAATCTTGCATCTCGGTCAC+TGG 0.522615 1_2:+28783845 Msa0065930:intron
ACTACTAACTCCTGCTGTGA+TGG 0.528455 1_2:+28785370 Msa0065930:CDS
AACATATATGTGCATTCACT+GGG 0.532518 1_2:+28785474 Msa0065930:three_prime_UTR
TCAATCTTCTCCATCACAGC+AGG 0.538974 1_2:-28785380 None:intergenic
CTTACTGGAAAATACAAGAA+AGG 0.542569 1_2:+28783222 Msa0065930:CDS
ATGGAATTGGTCTCACCACT+TGG 0.547573 1_2:+28783178 Msa0065930:CDS
AAATCCCAGTCTTAAATCCA+TGG 0.548325 1_2:-28785613 None:intergenic
AACCGACGAAACATTAGGAT+TGG 0.550427 1_2:-28783961 None:intergenic
TGCTGTGATGGAGAAGATTG+AGG 0.560987 1_2:+28785382 Msa0065930:CDS
CAAAGCAAAACGGCTGTCTG+GGG 0.571435 1_2:-28783253 None:intergenic
TGCCAATCCTAATGTTTCGT+CGG 0.571437 1_2:+28783959 Msa0065930:CDS
TTGGAATACTTAATAAAAGG+AGG 0.584186 1_2:-28785567 None:intergenic
AATGTTTCGTCGGTTATCTG+TGG 0.587284 1_2:+28783969 Msa0065930:CDS
TAGCACAGCTTTCAATTGCG+TGG 0.595497 1_2:+28783931 Msa0065930:CDS
TGTTGTAGAATCTTGCATCT+CGG 0.602036 1_2:+28783838 Msa0065930:intron
AAAGCAAAACGGCTGTCTGG+GGG 0.620635 1_2:-28783252 None:intergenic
GTAAGGACTCCAGACGCAAG+TGG 0.627062 1_2:-28783204 None:intergenic
AAGCCAATAGCAGATGAACT+GGG 0.634352 1_2:+28783900 Msa0065930:CDS
ATATATGTGCATTCACTGGG+AGG 0.658521 1_2:+28785477 Msa0065930:three_prime_UTR
AGACGCAAGTGGACTCCAAG+TGG 0.662326 1_2:-28783193 None:intergenic
AATTCCATAACTAGAATACA+GGG 0.664677 1_2:-28783163 None:intergenic
ATTCCATAACTAGAATACAG+GGG 0.746347 1_2:-28783162 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAAAAGAAAACTAAAAA+AGG - chr1_2:28783309-28783328 None:intergenic 10.0%
!! ACAAGGTAATAAAAAATTTA+AGG + chr1_2:28783283-28783302 Msa0065930:intron 15.0%
!! TCATAAACTACATTTAAATT+TGG - chr1_2:28783399-28783418 None:intergenic 15.0%
!!! ATTTGTTAAATTCCTTAAAT+GGG - chr1_2:28784584-28784603 None:intergenic 15.0%
!!! TATTTGTTAAATTCCTTAAA+TGG - chr1_2:28784585-28784604 None:intergenic 15.0%
!! TTAATAAAAAATTAAACCCT+GGG + chr1_2:28784951-28784970 Msa0065930:intron 15.0%
!!! TTCTTTCTTTTAAAATATCA+GGG + chr1_2:28785688-28785707 Msa0065930:three_prime_UTR 15.0%
!!! GAATAACATAGAAACTATTT+TGG + chr1_2:28783515-28783534 Msa0065930:intron 20.0%
!! AATCTATATGTCAATTTAGA+AGG - chr1_2:28783721-28783740 None:intergenic 20.0%
!! ACTAATAAATACTGAGAATT+AGG + chr1_2:28784264-28784283 Msa0065930:intron 20.0%
!! CTAATAAATACTGAGAATTA+GGG + chr1_2:28784265-28784284 Msa0065930:intron 20.0%
!! TAATAAATACTGAGAATTAG+GGG + chr1_2:28784266-28784285 Msa0065930:intron 20.0%
!! CAAGAAATTAAAACAAAGAA+TGG + chr1_2:28784399-28784418 Msa0065930:intron 20.0%
!!! AATTAGACTAGCTTTATATA+AGG + chr1_2:28784429-28784448 Msa0065930:intron 20.0%
!!! ATTAGACTAGCTTTATATAA+GGG + chr1_2:28784430-28784449 Msa0065930:intron 20.0%
!! GAATTTAACAAATATTGAAC+AGG + chr1_2:28784591-28784610 Msa0065930:intron 20.0%
!! AACATAAAGTATGTGATATA+AGG - chr1_2:28784725-28784744 None:intergenic 20.0%
!! GTTAATAAAAAATTAAACCC+TGG + chr1_2:28784950-28784969 Msa0065930:intron 20.0%
!!! TTTTTCTCTTCTTTATTCTT+TGG - chr1_2:28785038-28785057 None:intergenic 20.0%
!! AGATAAAAGTTCCATATAAA+TGG - chr1_2:28785081-28785100 None:intergenic 20.0%
!!! CATTTGGAATACTTAATAAA+AGG - chr1_2:28785573-28785592 None:intergenic 20.0%
!!! TAATATATGCTTATGGTTTT+TGG + chr1_2:28785661-28785680 Msa0065930:three_prime_UTR 20.0%
!!! GTTCTTTCTTTTAAAATATC+AGG + chr1_2:28785687-28785706 Msa0065930:three_prime_UTR 20.0%
! AATTCCATAACTAGAATACA+GGG - chr1_2:28783166-28783185 None:intergenic 25.0%
!! TGTAATTTTCCAAAGCAAAA+CGG - chr1_2:28783266-28783285 None:intergenic 25.0%
!!! TTTTGCTTTGGAAAATTACA+AGG + chr1_2:28783266-28783285 Msa0065930:CDS 25.0%
! ATAGAGATCATAATATGTGA+GGG + chr1_2:28783551-28783570 Msa0065930:intron 25.0%
!! TGCTGATATGTGTAAATTTT+CGG - chr1_2:28783676-28783695 None:intergenic 25.0%
!! ATTCTCAAGACATTTTTACA+AGG + chr1_2:28784023-28784042 Msa0065930:intron 25.0%
!! ATTTTTACAAGGACATCATT+TGG + chr1_2:28784034-28784053 Msa0065930:intron 25.0%
!! TTTTTACAAGGACATCATTT+GGG + chr1_2:28784035-28784054 Msa0065930:intron 25.0%
!!! AATGTTCTTTGTTTTCCTAA+TGG + chr1_2:28784210-28784229 Msa0065930:intron 25.0%
! TGCAATAAAATAGAAGACAA+AGG + chr1_2:28784623-28784642 Msa0065930:intron 25.0%
! TAAAGTATGTGATATAAGGA+TGG - chr1_2:28784721-28784740 None:intergenic 25.0%
! GATATACATGTCCATTTATA+TGG + chr1_2:28785067-28785086 Msa0065930:intron 25.0%
!! TTGATGTCATTCTTAATTTG+AGG + chr1_2:28785242-28785261 Msa0065930:intron 25.0%
! TTAGATTCATGAGAATATGA+AGG + chr1_2:28785331-28785350 Msa0065930:intron 25.0%
! TTGGAATACTTAATAAAAGG+AGG - chr1_2:28785570-28785589 None:intergenic 25.0%
!! AAGTATTCCAAATGTGTTTT+AGG + chr1_2:28785579-28785598 Msa0065930:three_prime_UTR 25.0%
! CCATAAGCATATATTAATCT+AGG - chr1_2:28785657-28785676 None:intergenic 25.0%
! CCTAGATTAATATATGCTTA+TGG + chr1_2:28785654-28785673 Msa0065930:three_prime_UTR 25.0%
!!! TAAAATATCAGGGTATTGTT+TGG + chr1_2:28785698-28785717 Msa0065930:three_prime_UTR 25.0%
ATTCCATAACTAGAATACAG+GGG - chr1_2:28783165-28783184 None:intergenic 30.0%
CAATTCCATAACTAGAATAC+AGG - chr1_2:28783167-28783186 None:intergenic 30.0%
! CTGTATTCTAGTTATGGAAT+TGG + chr1_2:28783165-28783184 Msa0065930:CDS 30.0%
! CTTTCTTGTATTTTCCAGTA+AGG - chr1_2:28783224-28783243 None:intergenic 30.0%
CTTACTGGAAAATACAAGAA+AGG + chr1_2:28783222-28783241 Msa0065930:CDS 30.0%
! TTCTTCACTGTTTCTTATGA+TGG + chr1_2:28783344-28783363 Msa0065930:intron 30.0%
TGATAGAAGATGGAATGATT+TGG + chr1_2:28783424-28783443 Msa0065930:intron 30.0%
GATAGAAGATGGAATGATTT+GGG + chr1_2:28783425-28783444 Msa0065930:intron 30.0%
! ATTTGGGTTTATGAAGAGTT+AGG + chr1_2:28783441-28783460 Msa0065930:intron 30.0%
ATGAAGAGTTAGGTCAATTT+CGG + chr1_2:28783451-28783470 Msa0065930:intron 30.0%
!! ACTATTTTGGATCGTTAACT+AGG + chr1_2:28783528-28783547 Msa0065930:intron 30.0%
GATAGAGATCATAATATGTG+AGG + chr1_2:28783550-28783569 Msa0065930:intron 30.0%
TAGAGATCATAATATGTGAG+GGG + chr1_2:28783552-28783571 Msa0065930:intron 30.0%
GCAATCAATCATTACAGTTA+CGG - chr1_2:28783699-28783718 None:intergenic 30.0%
TTCTATCCATCATACAACAA+TGG - chr1_2:28783750-28783769 None:intergenic 30.0%
!! TGTTGTATGATGGATAGAAA+TGG + chr1_2:28783751-28783770 Msa0065930:intron 30.0%
! GGATAGAAATGGAATTGTTT+TGG + chr1_2:28783762-28783781 Msa0065930:intron 30.0%
CTCAGTAATCTAAACAAAAG+AGG - chr1_2:28783788-28783807 None:intergenic 30.0%
! TGAAAAGTTTTCATGCCATT+AGG - chr1_2:28784228-28784247 None:intergenic 30.0%
!! TGGTTTTTGTTTCGATTTTC+TGG + chr1_2:28784295-28784314 Msa0065930:intron 30.0%
! AGTGACGTTTTCGTTATTAA+AGG + chr1_2:28784370-28784389 Msa0065930:intron 30.0%
! ACTGAAAGTACAACCAATTA+TGG + chr1_2:28784551-28784570 Msa0065930:intron 30.0%
! ATTGGTTCAATCCTTTCAAT+AGG - chr1_2:28784863-28784882 None:intergenic 30.0%
AGGATTGAACCAATATATGT+TGG + chr1_2:28784869-28784888 Msa0065930:intron 30.0%
CCACTTAAACCAACATATAT+TGG - chr1_2:28784881-28784900 None:intergenic 30.0%
!! CCAATATATGTTGGTTTAAG+TGG + chr1_2:28784878-28784897 Msa0065930:intron 30.0%
CAAACTTGTGACTTTACTTA+AGG - chr1_2:28784918-28784937 None:intergenic 30.0%
TCGGGTAATAACTGATATAA+GGG - chr1_2:28784988-28785007 None:intergenic 30.0%
ATCGGGTAATAACTGATATA+AGG - chr1_2:28784989-28785008 None:intergenic 30.0%
TTTATTCTTTGGTCTAACCT+TGG - chr1_2:28785027-28785046 None:intergenic 30.0%
ATAAGGGGGCAAAATTATAA+TGG - chr1_2:28785145-28785164 None:intergenic 30.0%
ACACACACACATACAAATAA+GGG - chr1_2:28785161-28785180 None:intergenic 30.0%
! GTTTTCCTATGCTTAAATCT+AGG + chr1_2:28785204-28785223 Msa0065930:intron 30.0%
GATTCGTACAGATGAATTTA+AGG + chr1_2:28785434-28785453 Msa0065930:exon 30.0%
AACATATATGTGCATTCACT+GGG + chr1_2:28785474-28785493 Msa0065930:three_prime_UTR 30.0%
ATCAAGTGAAACGAAACAAT+TGG - chr1_2:28785737-28785756 None:intergenic 30.0%
AGAAACAGTGAAGAAAGACA+CGG - chr1_2:28783339-28783358 None:intergenic 35.0%
! TATGAGAGCATGATAGAAGA+TGG + chr1_2:28783414-28783433 Msa0065930:intron 35.0%
TGTTGTAGAATCTTGCATCT+CGG + chr1_2:28783838-28783857 Msa0065930:intron 35.0%
TTCCACATGTAACAGAAAGT+TGG - chr1_2:28784191-28784210 None:intergenic 35.0%
AAAGATGCAAATAGACTGTG+AGG + chr1_2:28784488-28784507 Msa0065930:intron 35.0%
AATGGGTCTTCATCCATAAT+TGG - chr1_2:28784567-28784586 None:intergenic 35.0%
TATGGATGAAGACCCATTTA+AGG + chr1_2:28784569-28784588 Msa0065930:intron 35.0%
TTGAGTACTAGAGAGAGAAA+AGG + chr1_2:28784654-28784673 Msa0065930:intron 35.0%
TGAGTACTAGAGAGAGAAAA+GGG + chr1_2:28784655-28784674 Msa0065930:intron 35.0%
TATTGACAATGCTTCTTCGA+AGG - chr1_2:28784827-28784846 None:intergenic 35.0%
GGGTAATAACTGATATAAGG+GGG - chr1_2:28784986-28785005 None:intergenic 35.0%
CGGGTAATAACTGATATAAG+GGG - chr1_2:28784987-28785006 None:intergenic 35.0%
ACACACACATACAAATAAGG+GGG - chr1_2:28785159-28785178 None:intergenic 35.0%
CACACACACATACAAATAAG+GGG - chr1_2:28785160-28785179 None:intergenic 35.0%
CACACACACACATACAAATA+AGG - chr1_2:28785162-28785181 None:intergenic 35.0%
GTTCTCCTAGATTTAAGCAT+AGG - chr1_2:28785212-28785231 None:intergenic 35.0%
GAATGACATCAATTGCTTTC+AGG - chr1_2:28785234-28785253 None:intergenic 35.0%
CAACATATATGTGCATTCAC+TGG + chr1_2:28785473-28785492 Msa0065930:three_prime_UTR 35.0%
CAATGCTCCTAAAACACATT+TGG - chr1_2:28785589-28785608 None:intergenic 35.0%
! TACTCCATGGATTTAAGACT+GGG + chr1_2:28785609-28785628 Msa0065930:three_prime_UTR 35.0%
AAATCCCAGTCTTAAATCCA+TGG - chr1_2:28785616-28785635 None:intergenic 35.0%
! ATGGATTTAAGACTGGGATT+TGG + chr1_2:28785615-28785634 Msa0065930:three_prime_UTR 35.0%
! TGGATTTAAGACTGGGATTT+GGG + chr1_2:28785616-28785635 Msa0065930:three_prime_UTR 35.0%
TGTTCACGGTAATAACTTCA+TGG - chr1_2:28785760-28785779 None:intergenic 35.0%
! ACTTAATCACGTCTTGTTCA+CGG - chr1_2:28785774-28785793 None:intergenic 35.0%
! GTCTGCTTGAATTGACTTAT+TGG + chr1_2:28785794-28785813 Msa0065930:three_prime_UTR 35.0%
! CTTCCCCTGTATTCTAGTTA+TGG + chr1_2:28783159-28783178 Msa0065930:CDS 40.0%
CTGACACCATTGTTGTATGA+TGG + chr1_2:28783741-28783760 Msa0065930:intron 40.0%
TACAACACACGAGGAAAAGT+GGG - chr1_2:28783825-28783844 None:intergenic 40.0%
!! GGTTGATGATGTGCTCAAAA+AGG + chr1_2:28783866-28783885 Msa0065930:CDS 40.0%
GATGTGCTCAAAAAGGTTGA+TGG + chr1_2:28783873-28783892 Msa0065930:CDS 40.0%
AAGCCAATAGCAGATGAACT+GGG + chr1_2:28783900-28783919 Msa0065930:CDS 40.0%
GCAATTGAAAGCTGTGCTAA+TGG - chr1_2:28783930-28783949 None:intergenic 40.0%
TGCCAATCCTAATGTTTCGT+CGG + chr1_2:28783959-28783978 Msa0065930:CDS 40.0%
AACCGACGAAACATTAGGAT+TGG - chr1_2:28783964-28783983 None:intergenic 40.0%
CAGATAACCGACGAAACATT+AGG - chr1_2:28783969-28783988 None:intergenic 40.0%
! AATGTTTCGTCGGTTATCTG+TGG + chr1_2:28783969-28783988 Msa0065930:CDS 40.0%
CAAGAAATACTTGTAGCAGC+TGG + chr1_2:28784122-28784141 Msa0065930:intron 40.0%
CTCCAACTTTCTGTTACATG+TGG + chr1_2:28784186-28784205 Msa0065930:intron 40.0%
! CTGAGAATTAGGGGACTTTT+TGG + chr1_2:28784275-28784294 Msa0065930:intron 40.0%
GCAGAAGGAATTACTAGGTA+TGG - chr1_2:28784699-28784718 None:intergenic 40.0%
TGTGATATAAGGATGGCAGA+AGG - chr1_2:28784714-28784733 None:intergenic 40.0%
AGGAATGTGGTAGAGAAGAA+AGG - chr1_2:28784749-28784768 None:intergenic 40.0%
! TAACAGGTTACTTTCAACCC+AGG - chr1_2:28784788-28784807 None:intergenic 40.0%
! TGGGTTGAAAGTAACCTGTT+AGG + chr1_2:28784787-28784806 Msa0065930:intron 40.0%
AAGTAACCTGTTAGGTCACT+TGG + chr1_2:28784795-28784814 Msa0065930:intron 40.0%
TCAACAGTCGTCCTATTGAA+AGG + chr1_2:28784849-28784868 Msa0065930:intron 40.0%
! TGGCATAATCTGAGGTGAAT+CGG - chr1_2:28785007-28785026 None:intergenic 40.0%
ATTCACCTCAGATTATGCCA+AGG + chr1_2:28785007-28785026 Msa0065930:intron 40.0%
TCTAACCTTGGCATAATCTG+AGG - chr1_2:28785015-28785034 None:intergenic 40.0%
! GCATAACTCTTTTGCAAGAG+TGG + chr1_2:28785264-28785283 Msa0065930:intron 40.0%
ATATATGTGCATTCACTGGG+AGG + chr1_2:28785477-28785496 Msa0065930:three_prime_UTR 40.0%
GTTTCATGATCCCGTTTGTT+GGG + chr1_2:28785499-28785518 Msa0065930:three_prime_UTR 40.0%
AAAGTTGCACTCCCAACAAA+CGG - chr1_2:28785513-28785532 None:intergenic 40.0%
!! TTTAGGAGCATTGTACTCCA+TGG + chr1_2:28785596-28785615 Msa0065930:three_prime_UTR 40.0%
! GTACTCCATGGATTTAAGAC+TGG + chr1_2:28785608-28785627 Msa0065930:three_prime_UTR 40.0%
! ATGGAATTGGTCTCACCACT+TGG + chr1_2:28783178-28783197 Msa0065930:CDS 45.0%
CTACAACACACGAGGAAAAG+TGG - chr1_2:28783826-28783845 None:intergenic 45.0%
GCAAGATTCTACAACACACG+AGG - chr1_2:28783834-28783853 None:intergenic 45.0%
GAAGCCAATAGCAGATGAAC+TGG + chr1_2:28783899-28783918 Msa0065930:CDS 45.0%
TAGCACAGCTTTCAATTGCG+TGG + chr1_2:28783931-28783950 Msa0065930:CDS 45.0%
GGATGGCAGAAGGAATTACT+AGG - chr1_2:28784704-28784723 None:intergenic 45.0%
ACTGAACCAAGTGACCTAAC+AGG - chr1_2:28784804-28784823 None:intergenic 45.0%
! GGCATAATCTGAGGTGAATC+GGG - chr1_2:28785006-28785025 None:intergenic 45.0%
ACTACTAACTCCTGCTGTGA+TGG + chr1_2:28785370-28785389 Msa0065930:CDS 45.0%
TCAATCTTCTCCATCACAGC+AGG - chr1_2:28785383-28785402 None:intergenic 45.0%
! TGCTGTGATGGAGAAGATTG+AGG + chr1_2:28785382-28785401 Msa0065930:CDS 45.0%
GGTTTCATGATCCCGTTTGT+TGG + chr1_2:28785498-28785517 Msa0065930:three_prime_UTR 45.0%
AAGTTGCACTCCCAACAAAC+GGG - chr1_2:28785512-28785531 None:intergenic 45.0%
AAAGCAAAACGGCTGTCTGG+GGG - chr1_2:28783255-28783274 None:intergenic 50.0%
CAAAGCAAAACGGCTGTCTG+GGG - chr1_2:28783256-28783275 None:intergenic 50.0%
CCAAAGCAAAACGGCTGTCT+GGG - chr1_2:28783257-28783276 None:intergenic 50.0%
TCCAAAGCAAAACGGCTGTC+TGG - chr1_2:28783258-28783277 None:intergenic 50.0%
GAATCTTGCATCTCGGTCAC+TGG + chr1_2:28783845-28783864 Msa0065930:intron 50.0%
ACGCCCAGTTCATCTGCTAT+TGG - chr1_2:28783906-28783925 None:intergenic 50.0%
TGGCGCTACAAAAGAGTCTC+AGG + chr1_2:28783989-28784008 Msa0065930:CDS 50.0%
TTCTCTACCACATTCCTCCG+TGG + chr1_2:28784752-28784771 Msa0065930:intron 50.0%
TCAAGAACCACGGAGGAATG+TGG - chr1_2:28784762-28784781 None:intergenic 50.0%
TCCGTGGTTCTTGAGTTCCT+GGG + chr1_2:28784768-28784787 Msa0065930:intron 50.0%
ACCCAGGAACTCAAGAACCA+CGG - chr1_2:28784772-28784791 None:intergenic 50.0%
! AGACGCAAGTGGACTCCAAG+TGG - chr1_2:28783196-28783215 None:intergenic 55.0%
ACTTGGAGTCCACTTGCGTC+TGG + chr1_2:28783195-28783214 Msa0065930:CDS 55.0%
GTAAGGACTCCAGACGCAAG+TGG - chr1_2:28783207-28783226 None:intergenic 55.0%
! CTTGCGTCTGGAGTCCTTAC+TGG + chr1_2:28783207-28783226 Msa0065930:CDS 55.0%
!! CCCAGACAGCCGTTTTGCTT+TGG + chr1_2:28783254-28783273 Msa0065930:CDS 55.0%
CAGGAACTCAAGAACCACGG+AGG - chr1_2:28784769-28784788 None:intergenic 55.0%
CTCCGTGGTTCTTGAGTTCC+TGG + chr1_2:28784767-28784786 Msa0065930:intron 55.0%
AAGGGGGCAAAACTCTCCCA+GGG - chr1_2:28784970-28784989 None:intergenic 55.0%
TAAGGGGGCAAAACTCTCCC+AGG - chr1_2:28784971-28784990 None:intergenic 55.0%
CTGTACGAATCAGCGCGCTT+TGG - chr1_2:28785425-28785444 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 28783144 28785843 28783144 ID=Msa0065930;Name=Msa0065930
chr1_2 mRNA 28783144 28785843 28783144 ID=Msa0065930-mRNA-1;Parent=Msa0065930;Name=Msa0065930-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|0.66|1|1|1|3|395|140
chr1_2 exon 28783144 28783287 28783144 ID=Msa0065930-mRNA-1:exon:9791;Parent=Msa0065930-mRNA-1
chr1_2 exon 28783846 28784010 28783846 ID=Msa0065930-mRNA-1:exon:9792;Parent=Msa0065930-mRNA-1
chr1_2 exon 28785335 28785843 28785335 ID=Msa0065930-mRNA-1:exon:9793;Parent=Msa0065930-mRNA-1
chr1_2 CDS 28783144 28783287 28783144 ID=Msa0065930-mRNA-1:cds;Parent=Msa0065930-mRNA-1
chr1_2 CDS 28783846 28784010 28783846 ID=Msa0065930-mRNA-1:cds;Parent=Msa0065930-mRNA-1
chr1_2 CDS 28785335 28785448 28785335 ID=Msa0065930-mRNA-1:cds;Parent=Msa0065930-mRNA-1
chr1_2 three_prime_UTR 28785449 28785843 28785449 ID=Msa0065930-mRNA-1:three_prime_utr;Parent=Msa0065930-mRNA-1
Gene Sequence

>Msa0065930

GTTGAGTCTGAGTATCTTCCCCTGTATTCTAGTTATGGAATTGGTCTCACCACTTGGAGTCCACTTGCGTCTGGAGTCCTTACTGGAAAATACAAGAAAGGAGTTATTCCCCCAGACAGCCGTTTTGCTTTGGAAAATTACAAGAATCTTGCATCTCGGTCACTGGTTGATGATGTGCTCAAAAAGGTTGATGGTCTGAAGCCAATAGCAGATGAACTGGGCGTGCCATTAGCACAGCTTTCAATTGCGTGGTGTGCTGCCAATCCTAATGTTTCGTCGGTTATCTGTGGCGCTACAAAAGAGTCTCAGATTCATGAGAATATGAAGGCTATTGAAGTCATTAAACTACTAACTCCTGCTGTGATGGAGAAGATTGAGGCTGTTGTTCAAAGCAAACCAAAGCGCGCTGATTCGTACAGATGA
Protein sequence

>Msa0065930

VESEYLPLYSSYGIGLTTWSPLASGVLTGKYKKGVIPPDSRFALENYKNLASRSLVDDVLKKVDGLKPIADELGVPLAQLSIAWCAANPNVSSVICGATKESQIHENMKAIEVIKLLTPAVMEKIEAVVQSKPKRADSYR*