AlfalfaGEDB | Msa0315170

M. sativa cultivar zhongmu-4 / Msa0315170

KEGG: K01802
GO: GO:0000413 GO:0003674 GO:0003755 GO:0003824 GO:0005488 GO:0005527 GO:0005528 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006457 GO:0006464 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016853 GO:0016859 GO:0018193 GO:0018208 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0061077 GO:0071704 GO:0097159 GO:0140096 GO:1901363 GO:1901564
PFAM: PF00254.31

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0315170	XP_003593250.1	99.107	112	1	0	1	112	1	112	2.21e-75	228

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0315170	sp\|O04287\|FKB12_VICFA	89.286	112	12	0	1	112	1	112	5.24e-72	213

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0315170	B7FFD7	99.107	112	1	0	1	112	1	112	1.05e-75	228

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001450	Msa0315170	0.801997	6.913933e-49	-8.615850e-47
Msa0056430	Msa0315170	0.810667	1.028496e-50	-8.615850e-47
Msa0056470	Msa0315170	0.815898	7.302720e-52	-8.615850e-47
Msa0064380	Msa0315170	0.825682	4.102437e-54	-8.615850e-47
Msa0065930	Msa0315170	0.802158	6.405837e-49	-8.615850e-47
Msa0149200	Msa0315170	0.831347	1.757202e-55	-8.615850e-47
Msa0280610	Msa0315170	0.807143	5.836705e-50	-8.615850e-47
Msa0315170	Msa0369450	0.805304	1.423810e-49	-8.615850e-47
Msa0315170	Msa0390030	0.804293	2.315797e-49	-8.615850e-47
Msa0315170	Msa0432210	0.809312	2.013765e-50	-8.615850e-47
Msa0315170	Msa0469990	0.802289	6.020321e-49	-8.615850e-47
Msa0315170	Msa0518390	0.812779	3.570170e-51	-8.615850e-47
Msa0315170	Msa0518450	0.825978	3.489709e-54	-8.615850e-47
Msa0315170	Msa0521050	0.811534	6.673130e-51	-8.615850e-47
Msa0315170	Msa0552810	0.806139	9.510038e-50	-8.615850e-47
Msa0315170	Msa0651050	0.815279	1.002750e-51	-8.615850e-47
Msa0315170	Msa0692840	0.815762	7.831037e-52	-8.615850e-47
Msa0315170	Msa0784200	0.853592	2.157957e-61	-8.615850e-47
Msa0315170	Msa0818310	0.854805	9.638381e-62	-8.615850e-47
Msa0315170	Msa0858770	0.852945	3.309039e-61	-8.615850e-47
Msa0315170	Msa0881460	0.807943	3.948523e-50	-8.615850e-47
Msa0315170	Msa1050210	0.803675	3.113375e-49	-8.615850e-47
Msa0315170	Msa1078270	0.837665	4.546395e-57	-8.615850e-47
Msa0315170	Msa1081990	0.816908	4.339632e-52	-8.615850e-47
Msa0315170	Msa1123500	0.814008	1.916749e-51	-8.615850e-47
Msa0315170	Msa1123570	0.846050	2.769891e-59	-8.615850e-47
Msa0315170	Msa1126510	0.800450	1.432859e-48	-8.615850e-47
Msa0315170	Msa1158570	0.823568	1.291644e-53	-8.615850e-47
Msa0315170	Msa1165940	0.843072	1.754673e-58	-8.615850e-47
Msa0315170	Msa1169770	0.834255	3.330604e-56	-8.615850e-47
Msa0315170	Msa1418340	0.800044	1.733744e-48	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0315170	MtrunA17_Chr2g0279511	99.107	112	1	0	1	112	1	112	2.03e-79	228
Msa0315170	MtrunA17_Chr8g0377771	45.455	110	54	4	2	110	38	142	8.27e-20	83.2
Msa0315170	MtrunA17_Chr8g0377771	36.036	111	65	3	3	110	272	379	5.21e-14	66.6
Msa0315170	MtrunA17_Chr5g0435521	40.000	110	63	3	2	110	12	119	9.80e-20	79.7
Msa0315170	MtrunA17_Chr8g0352971	41.905	105	50	3	11	111	9	106	1.03e-17	77.0
Msa0315170	MtrunA17_Chr7g0234801	45.263	95	47	3	16	110	49	138	2.67e-17	75.9
Msa0315170	MtrunA17_Chr8g0339141	46.316	95	46	3	16	110	55	144	5.69e-17	75.1
Msa0315170	MtrunA17_Chr7g0255551	44.037	109	55	4	2	109	21	124	2.25e-16	73.2
Msa0315170	MtrunA17_Chr4g0052911	36.364	110	56	4	11	110	133	238	2.71e-12	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0315170	AT5G64350.1	85.714	112	16	0	1	112	1	112	1.38e-70	206
Msa0315170	AT3G55520.2	35.780	109	69	1	2	110	12	119	1.51e-17	74.3
Msa0315170	AT3G55520.3	35.780	109	69	1	2	110	12	119	1.51e-17	74.3
Msa0315170	AT3G55520.1	35.780	109	69	1	2	110	12	119	1.51e-17	74.3
Msa0315170	AT5G48570.1	40.000	110	60	4	2	110	47	151	1.00e-15	72.0
Msa0315170	AT3G25230.2	40.367	109	59	4	2	109	39	142	4.87e-15	70.1
Msa0315170	AT3G25230.1	40.367	109	59	4	2	109	39	142	6.25e-15	69.7
Msa0315170	AT4G25340.1	41.176	102	53	4	12	112	382	477	5.61e-14	67.0
Msa0315170	AT5G45680.1	40.367	109	51	4	11	109	101	205	4.20e-13	63.2
Msa0315170	AT5G05420.1	40.196	102	53	4	12	112	49	143	9.33e-13	60.8

Find 50 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTCTCCAATATTAATATT+TGG	0.175040	2_4:-11376292	Msa0315170:three_prime_UTR
ATTTGTTGATCAATATTATT+TGG	0.184739	2_4:-11376224	Msa0315170:three_prime_UTR
CGCTAGTGGCTTTCCTGCTT+GGG	0.208113	2_4:-11376525	Msa0315170:CDS
ACACTACCAAATATTAATAT+TGG	0.265284	2_4:+11376286	None:intergenic
GTGACCTTTCTCAGAAATTC+TGG	0.269473	2_4:-11377672	Msa0315170:CDS
GATAAAGAAAATAAAGAAAC+AGG	0.282556	2_4:+11376248	None:intergenic
TACTGAAGGACAAACTAAAA+AGG	0.293548	2_4:+11376443	None:intergenic
TGCTCTCCTGATTATGCTTA+TGG	0.301017	2_4:-11376548	Msa0315170:CDS
TCGGAGAAGTTGCTCGTCTT+CGG	0.329775	2_4:-11377128	Msa0315170:CDS
AATACAACCCAACTCTGTTT+TGG	0.332199	2_4:-11376501	Msa0315170:CDS
GCGCTAGTGGCTTTCCTGCT+TGG	0.337892	2_4:-11376526	Msa0315170:CDS
CGGAGAAGTTGCTCGTCTTC+GGG	0.345948	2_4:-11377127	Msa0315170:intron
AATCCATTCACTTTCAAAAT+CGG	0.376739	2_4:-11377542	Msa0315170:CDS
GAAAGAAGGAAGAAGAAAGA+TGG	0.415250	2_4:-11377952	Msa0315170:exon
AAAGAAGGAAGAAGAAAGAT+GGG	0.430314	2_4:-11377951	Msa0315170:exon
GTTGGCTTAGGACCGTTTCC+AGG	0.437240	2_4:+11377906	Msa0315180:intron
TATTTGGCAATGCATGGTCT+TGG	0.437640	2_4:-11376208	Msa0315170:three_prime_UTR
GAGAGTGTGCTCACCGTATC+CGG	0.439075	2_4:+11377854	Msa0315180:CDS
CTAGCGCCATAAGCATAATC+AGG	0.449393	2_4:+11376542	None:intergenic
TCAAACTCCAAAACAGAGTT+GGG	0.461799	2_4:+11376494	None:intergenic
CTCAAACTCCAAAACAGAGT+TGG	0.473985	2_4:+11376493	None:intergenic
GGTGTCATTGGCATGCAAAT+CGG	0.478078	2_4:-11377147	Msa0315170:CDS
AAAGTGAATGGATTCTGGCC+AGG	0.495829	2_4:+11377551	None:intergenic
GCAGATATCACTCAAAGTGC+AGG	0.504337	2_4:+11376365	None:intergenic
CAAATCGTTCGACCTGGAAA+CGG	0.515678	2_4:-11377918	Msa0315170:CDS
ACCTCCAGAATTTCTGAGAA+AGG	0.516160	2_4:+11377668	None:intergenic
ACTGTCCACTGCACCGGATA+CGG	0.518197	2_4:-11377867	Msa0315170:intron
GCATAAGATCGAGTGAAAGA+AGG	0.527705	2_4:-11377966	Msa0315170:five_prime_UTR
TTCACTTTCAAAATCGGCAA+AGG	0.528436	2_4:-11377536	Msa0315170:CDS
GGATGGGATGAAGGTGTCAT+TGG	0.529844	2_4:-11377159	Msa0315170:CDS
CAATATTATTTGGCAATGCA+TGG	0.531070	2_4:-11376214	Msa0315170:three_prime_UTR
ATACATGTCCACAGAAATGT+AGG	0.543594	2_4:+11376397	None:intergenic
TATTACAGTACAAAAGATCC+TGG	0.543772	2_4:-11377569	Msa0315170:intron
ATTGGAGAGTTTGAGATCAG+TGG	0.545467	2_4:+11376304	None:intergenic
GATTATGCTTATGGCGCTAG+TGG	0.545830	2_4:-11376539	Msa0315170:CDS
AACGGTCCTAAGCCAACTCC+TGG	0.550713	2_4:-11377900	Msa0315170:CDS
AACGTCACTGTCCACTGCAC+CGG	0.551030	2_4:-11377873	Msa0315170:CDS
TTGTGTGTAGGATGGGATGA+AGG	0.556041	2_4:-11377168	Msa0315170:intron
TTGGGTTGTATTCCCCAAGC+AGG	0.558631	2_4:+11376512	None:intergenic
CAGATATCACTCAAAGTGCA+GGG	0.569024	2_4:+11376366	None:intergenic
GCTAGTGGCTTTCCTGCTTG+GGG	0.575669	2_4:-11376524	Msa0315170:CDS
ACCTTTCTCAGAAATTCTGG+AGG	0.582702	2_4:-11377669	Msa0315170:intron
GGCAAAGGTTCTGTCATCAA+AGG	0.586910	2_4:-11377521	Msa0315170:intron
TCATAATAATAATTTACTGA+AGG	0.596163	2_4:+11376429	None:intergenic
TTGAGTATCCTACATTTCTG+TGG	0.596981	2_4:-11376405	Msa0315170:three_prime_UTR
GCTCACCGTATCCGGTGCAG+TGG	0.598896	2_4:+11377862	Msa0315180:CDS
GAGAAGCAAATCGTTCGACC+TGG	0.621651	2_4:-11377924	Msa0315170:CDS
ACTTTGAGTGATATCTGCAG+TGG	0.642089	2_4:-11376360	Msa0315170:three_prime_UTR
ATAAAGAAAATAAAGAAACA+GGG	0.660507	2_4:+11376249	None:intergenic
AGATATCACTCAAAGTGCAG+GGG	0.704961	2_4:+11376367	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTTAATTCAAATTATAAG+GGG	+	chr2_4:11376384-11376403	None:intergenic	10.0%
!!	CTAATTTAATTCAAATTATA+AGG	+	chr2_4:11376386-11376405	None:intergenic	10.0%
!!	ACAATATTAAAAACAAAAAA+TGG	+	chr2_4:11376552-11376571	None:intergenic	10.0%
!!	AATTTAATTCAAATTATAAG+GGG	+	chr2_4:11376384-11376403	None:intergenic	10.0%
!!	CTAATTTAATTCAAATTATA+AGG	+	chr2_4:11376386-11376405	None:intergenic	10.0%
!!	ACAATATTAAAAACAAAAAA+TGG	+	chr2_4:11376552-11376571	None:intergenic	10.0%
!!	TAAAATGAAAGATAAGAAAT+GGG	+	chr2_4:11376357-11376376	None:intergenic	15.0%
!!	TTAAAATGAAAGATAAGAAA+TGG	+	chr2_4:11376358-11376377	None:intergenic	15.0%
!!	ATATAAGCTAAAATAGTTTA+TGG	+	chr2_4:11377203-11377222	None:intergenic	15.0%
!!	TCATAATAATAATTTACTGA+AGG	+	chr2_4:11377737-11377756	None:intergenic	15.0%
!!	ATAAAGAAAATAAAGAAACA+GGG	+	chr2_4:11377917-11377936	Msa0315180:intron	15.0%
!!!	ATTTGTTGATCAATATTATT+TGG	-	chr2_4:11377939-11377958	Msa0315170:exon	15.0%
!!	GAAATAAAAGAAAAATCTTT+AGG	+	chr2_4:11378028-11378047	Msa0315180:intron	15.0%
!!	TAAAATGAAAGATAAGAAAT+GGG	+	chr2_4:11376357-11376376	None:intergenic	15.0%
!!	TTAAAATGAAAGATAAGAAA+TGG	+	chr2_4:11376358-11376377	None:intergenic	15.0%
!!	ATATAAGCTAAAATAGTTTA+TGG	+	chr2_4:11377203-11377222	None:intergenic	15.0%
!!	TCATAATAATAATTTACTGA+AGG	+	chr2_4:11377737-11377756	None:intergenic	15.0%
!!	ATAAAGAAAATAAAGAAACA+GGG	+	chr2_4:11377917-11377936	Msa0315180:intron	15.0%
!!!	ATTTGTTGATCAATATTATT+TGG	-	chr2_4:11377939-11377958	Msa0315170:exon	15.0%
!!	GAAATAAAAGAAAAATCTTT+AGG	+	chr2_4:11378028-11378047	Msa0315180:intron	15.0%
!!	AAAATGAAAGATAAGAAATG+GGG	+	chr2_4:11376356-11376375	None:intergenic	20.0%
!!!	TAGTACTTTTACTTTATTTC+TGG	-	chr2_4:11376890-11376909	Msa0315170:intron	20.0%
!!	ATTTCTATGATAAGTGTTTA+TGG	-	chr2_4:11377319-11377338	Msa0315170:intron	20.0%
!!	AATGGAAATTCAACATATTA+AGG	+	chr2_4:11377381-11377400	None:intergenic	20.0%
!!	GTTGATACTTTGTTAAATAA+TGG	-	chr2_4:11377546-11377565	Msa0315170:CDS	20.0%
!!	AACTCTCCAATATTAATATT+TGG	-	chr2_4:11377871-11377890	Msa0315170:CDS	20.0%
!!	ACACTACCAAATATTAATAT+TGG	+	chr2_4:11377880-11377899	Msa0315180:CDS	20.0%
!!	GATAAAGAAAATAAAGAAAC+AGG	+	chr2_4:11377918-11377937	Msa0315180:intron	20.0%
!!	AAAATGAAAGATAAGAAATG+GGG	+	chr2_4:11376356-11376375	None:intergenic	20.0%
!!!	TAGTACTTTTACTTTATTTC+TGG	-	chr2_4:11376890-11376909	Msa0315170:intron	20.0%
!!	ATTTCTATGATAAGTGTTTA+TGG	-	chr2_4:11377319-11377338	Msa0315170:intron	20.0%
!!	AATGGAAATTCAACATATTA+AGG	+	chr2_4:11377381-11377400	None:intergenic	20.0%
!!	GTTGATACTTTGTTAAATAA+TGG	-	chr2_4:11377546-11377565	Msa0315170:CDS	20.0%
!!	AACTCTCCAATATTAATATT+TGG	-	chr2_4:11377871-11377890	Msa0315170:CDS	20.0%
!!	ACACTACCAAATATTAATAT+TGG	+	chr2_4:11377880-11377899	Msa0315180:CDS	20.0%
!!	GATAAAGAAAATAAAGAAAC+AGG	+	chr2_4:11377918-11377937	Msa0315180:intron	20.0%
!!!	AAATGGGGTTTTTAGTTTTA+GGG	+	chr2_4:11376341-11376360	None:intergenic	25.0%
!	AACAAAAAATGGAATGACAA+GGG	+	chr2_4:11376541-11376560	None:intergenic	25.0%
!	AAACAAAAAATGGAATGACA+AGG	+	chr2_4:11376542-11376561	None:intergenic	25.0%
!	AATCCATTCACTTTCAAAAT+CGG	-	chr2_4:11376621-11376640	Msa0315170:intron	25.0%
!!!	TTTATAACTTGTTACCACTT+TGG	-	chr2_4:11377086-11377105	Msa0315170:intron	25.0%
!!!	TTATAACTTGTTACCACTTT+GGG	-	chr2_4:11377087-11377106	Msa0315170:intron	25.0%
!!!	TTCAGTTTATATCGTTTTTG+TGG	-	chr2_4:11378000-11378019	Msa0315170:five_prime_UTR	25.0%
!!!	AAATGGGGTTTTTAGTTTTA+GGG	+	chr2_4:11376341-11376360	None:intergenic	25.0%
!	AACAAAAAATGGAATGACAA+GGG	+	chr2_4:11376541-11376560	None:intergenic	25.0%
!	AAACAAAAAATGGAATGACA+AGG	+	chr2_4:11376542-11376561	None:intergenic	25.0%
!	AATCCATTCACTTTCAAAAT+CGG	-	chr2_4:11376621-11376640	Msa0315170:intron	25.0%
!!!	TTTATAACTTGTTACCACTT+TGG	-	chr2_4:11377086-11377105	Msa0315170:intron	25.0%
!!!	TTATAACTTGTTACCACTTT+GGG	-	chr2_4:11377087-11377106	Msa0315170:intron	25.0%
!!!	TTCAGTTTATATCGTTTTTG+TGG	-	chr2_4:11378000-11378019	Msa0315170:five_prime_UTR	25.0%
	AAAGAAGGAAGAAGAAAGAT+GGG	-	chr2_4:11376212-11376231	Msa0315170:three_prime_UTR	30.0%
!!!	GAAATGGGGTTTTTAGTTTT+AGG	+	chr2_4:11376342-11376361	None:intergenic	30.0%
	TATTACAGTACAAAAGATCC+TGG	-	chr2_4:11376594-11376613	Msa0315170:intron	30.0%
!!	TTTTGAAAGTGAATGGATTC+TGG	+	chr2_4:11376620-11376639	None:intergenic	30.0%
!	GTGTAGAGTTTTTACACTAT+CGG	-	chr2_4:11376714-11376733	Msa0315170:intron	30.0%
	CAACATATTAAGGTCATGTT+TGG	+	chr2_4:11377371-11377390	None:intergenic	30.0%
	TACTGAAGGACAAACTAAAA+AGG	+	chr2_4:11377723-11377742	None:intergenic	30.0%
	CAATATTATTTGGCAATGCA+TGG	-	chr2_4:11377949-11377968	Msa0315170:exon	30.0%
	AAAGAAGGAAGAAGAAAGAT+GGG	-	chr2_4:11376212-11376231	Msa0315170:three_prime_UTR	30.0%
!!!	GAAATGGGGTTTTTAGTTTT+AGG	+	chr2_4:11376342-11376361	None:intergenic	30.0%
	TATTACAGTACAAAAGATCC+TGG	-	chr2_4:11376594-11376613	Msa0315170:intron	30.0%
!!	TTTTGAAAGTGAATGGATTC+TGG	+	chr2_4:11376620-11376639	None:intergenic	30.0%
!	GTGTAGAGTTTTTACACTAT+CGG	-	chr2_4:11376714-11376733	Msa0315170:intron	30.0%
	CAACATATTAAGGTCATGTT+TGG	+	chr2_4:11377371-11377390	None:intergenic	30.0%
	TACTGAAGGACAAACTAAAA+AGG	+	chr2_4:11377723-11377742	None:intergenic	30.0%
	CAATATTATTTGGCAATGCA+TGG	-	chr2_4:11377949-11377968	Msa0315170:exon	30.0%
	GAAAGAAGGAAGAAGAAAGA+TGG	-	chr2_4:11376211-11376230	Msa0315170:three_prime_UTR	35.0%
!!	TGATTGTGTTGATGATGATC+AGG	-	chr2_4:11376460-11376479	Msa0315170:exon	35.0%
!	TGATGATGATCAGGCAAAAA+CGG	-	chr2_4:11376469-11376488	Msa0315170:CDS	35.0%
!!!	TTGCCGATTTTGAAAGTGAA+TGG	+	chr2_4:11376627-11376646	None:intergenic	35.0%
	TTCACTTTCAAAATCGGCAA+AGG	-	chr2_4:11376627-11376646	Msa0315170:intron	35.0%
	TTACACACTTGTGAGACTTT+AGG	+	chr2_4:11376800-11376819	None:intergenic	35.0%
	CAATGTACATTCAGATGTGT+TGG	-	chr2_4:11376936-11376955	Msa0315170:intron	35.0%
	GTGAGTAAAACAAACAGCAA+TGG	+	chr2_4:11377399-11377418	None:intergenic	35.0%
!	GTTTTACTCACACACATACA+AGG	-	chr2_4:11377408-11377427	Msa0315170:intron	35.0%
	TATCAACAAGGTCAATGACA+GGG	+	chr2_4:11377586-11377605	None:intergenic	35.0%
	TTATCAACAAGGTCAATGAC+AGG	+	chr2_4:11377587-11377606	None:intergenic	35.0%
!	AATACAACCCAACTCTGTTT+TGG	-	chr2_4:11377662-11377681	Msa0315170:intron	35.0%
	TCAAACTCCAAAACAGAGTT+GGG	+	chr2_4:11377672-11377691	None:intergenic	35.0%
!!!	TGTTTTGGAGTTTGAGATTG+AGG	-	chr2_4:11377677-11377696	Msa0315170:CDS	35.0%
	TTGAGTATCCTACATTTCTG+TGG	-	chr2_4:11377758-11377777	Msa0315170:intron	35.0%
	ATACATGTCCACAGAAATGT+AGG	+	chr2_4:11377769-11377788	None:intergenic	35.0%
	GAAAGAAGGAAGAAGAAAGA+TGG	-	chr2_4:11376211-11376230	Msa0315170:three_prime_UTR	35.0%
!!	TGATTGTGTTGATGATGATC+AGG	-	chr2_4:11376460-11376479	Msa0315170:exon	35.0%
!	TGATGATGATCAGGCAAAAA+CGG	-	chr2_4:11376469-11376488	Msa0315170:CDS	35.0%
!!!	TTGCCGATTTTGAAAGTGAA+TGG	+	chr2_4:11376627-11376646	None:intergenic	35.0%
	TTCACTTTCAAAATCGGCAA+AGG	-	chr2_4:11376627-11376646	Msa0315170:intron	35.0%
	TTACACACTTGTGAGACTTT+AGG	+	chr2_4:11376800-11376819	None:intergenic	35.0%
	CAATGTACATTCAGATGTGT+TGG	-	chr2_4:11376936-11376955	Msa0315170:intron	35.0%
	GTGAGTAAAACAAACAGCAA+TGG	+	chr2_4:11377399-11377418	None:intergenic	35.0%
!	GTTTTACTCACACACATACA+AGG	-	chr2_4:11377408-11377427	Msa0315170:intron	35.0%
	TATCAACAAGGTCAATGACA+GGG	+	chr2_4:11377586-11377605	None:intergenic	35.0%
	TTATCAACAAGGTCAATGAC+AGG	+	chr2_4:11377587-11377606	None:intergenic	35.0%
!	AATACAACCCAACTCTGTTT+TGG	-	chr2_4:11377662-11377681	Msa0315170:intron	35.0%
	TCAAACTCCAAAACAGAGTT+GGG	+	chr2_4:11377672-11377691	None:intergenic	35.0%
!!!	TGTTTTGGAGTTTGAGATTG+AGG	-	chr2_4:11377677-11377696	Msa0315170:CDS	35.0%
	TTGAGTATCCTACATTTCTG+TGG	-	chr2_4:11377758-11377777	Msa0315170:intron	35.0%
	ATACATGTCCACAGAAATGT+AGG	+	chr2_4:11377769-11377788	None:intergenic	35.0%
	GCATAAGATCGAGTGAAAGA+AGG	-	chr2_4:11376197-11376216	Msa0315170:three_prime_UTR	40.0%
	GTGACCTTTCTCAGAAATTC+TGG	-	chr2_4:11376491-11376510	Msa0315170:CDS	40.0%
	ACCTTTCTCAGAAATTCTGG+AGG	-	chr2_4:11376494-11376513	Msa0315170:CDS	40.0%
!	ACCTCCAGAATTTCTGAGAA+AGG	+	chr2_4:11376498-11376517	None:intergenic	40.0%
!	TTCTCAGAAATTCTGGAGGT+TGG	-	chr2_4:11376498-11376517	Msa0315170:CDS	40.0%
	TCCAACTATCTGTTGTGTGT+AGG	-	chr2_4:11376983-11377002	Msa0315170:intron	40.0%
	TCCTACACACAACAGATAGT+TGG	+	chr2_4:11376987-11377006	None:intergenic	40.0%
	ACTATCTGTTGTGTGTAGGA+TGG	-	chr2_4:11376987-11377006	Msa0315170:intron	40.0%
	CTATCTGTTGTGTGTAGGAT+GGG	-	chr2_4:11376988-11377007	Msa0315170:intron	40.0%
	CAATTCAATCAAGCCCAAAG+TGG	+	chr2_4:11377103-11377122	None:intergenic	40.0%
	CACTTTGGGCTTGATTGAAT+TGG	-	chr2_4:11377101-11377120	Msa0315170:intron	40.0%
	TGGATGTGCAGGTCAATTAA+GGG	+	chr2_4:11377351-11377370	None:intergenic	40.0%
	TTGGATGTGCAGGTCAATTA+AGG	+	chr2_4:11377352-11377371	None:intergenic	40.0%
	GCACTGCAAGATTATCAACA+AGG	+	chr2_4:11377598-11377617	None:intergenic	40.0%
	TGCTCTCCTGATTATGCTTA+TGG	-	chr2_4:11377615-11377634	Msa0315170:intron	40.0%
	CTCAAACTCCAAAACAGAGT+TGG	+	chr2_4:11377673-11377692	None:intergenic	40.0%
	AGATATCACTCAAAGTGCAG+GGG	+	chr2_4:11377799-11377818	None:intergenic	40.0%
	CAGATATCACTCAAAGTGCA+GGG	+	chr2_4:11377800-11377819	None:intergenic	40.0%
	ACTTTGAGTGATATCTGCAG+TGG	-	chr2_4:11377803-11377822	Msa0315170:intron	40.0%
!	ATTGGAGAGTTTGAGATCAG+TGG	+	chr2_4:11377862-11377881	Msa0315180:CDS	40.0%
	TATTTGGCAATGCATGGTCT+TGG	-	chr2_4:11377955-11377974	Msa0315170:exon	40.0%
	GCATAAGATCGAGTGAAAGA+AGG	-	chr2_4:11376197-11376216	Msa0315170:three_prime_UTR	40.0%
	GTGACCTTTCTCAGAAATTC+TGG	-	chr2_4:11376491-11376510	Msa0315170:CDS	40.0%
	ACCTTTCTCAGAAATTCTGG+AGG	-	chr2_4:11376494-11376513	Msa0315170:CDS	40.0%
!	ACCTCCAGAATTTCTGAGAA+AGG	+	chr2_4:11376498-11376517	None:intergenic	40.0%
!	TTCTCAGAAATTCTGGAGGT+TGG	-	chr2_4:11376498-11376517	Msa0315170:CDS	40.0%
	TCCAACTATCTGTTGTGTGT+AGG	-	chr2_4:11376983-11377002	Msa0315170:intron	40.0%
	TCCTACACACAACAGATAGT+TGG	+	chr2_4:11376987-11377006	None:intergenic	40.0%
	ACTATCTGTTGTGTGTAGGA+TGG	-	chr2_4:11376987-11377006	Msa0315170:intron	40.0%
	CTATCTGTTGTGTGTAGGAT+GGG	-	chr2_4:11376988-11377007	Msa0315170:intron	40.0%
	CAATTCAATCAAGCCCAAAG+TGG	+	chr2_4:11377103-11377122	None:intergenic	40.0%
	CACTTTGGGCTTGATTGAAT+TGG	-	chr2_4:11377101-11377120	Msa0315170:intron	40.0%
	TGGATGTGCAGGTCAATTAA+GGG	+	chr2_4:11377351-11377370	None:intergenic	40.0%
	TTGGATGTGCAGGTCAATTA+AGG	+	chr2_4:11377352-11377371	None:intergenic	40.0%
	GCACTGCAAGATTATCAACA+AGG	+	chr2_4:11377598-11377617	None:intergenic	40.0%
	TGCTCTCCTGATTATGCTTA+TGG	-	chr2_4:11377615-11377634	Msa0315170:intron	40.0%
	CTCAAACTCCAAAACAGAGT+TGG	+	chr2_4:11377673-11377692	None:intergenic	40.0%
	AGATATCACTCAAAGTGCAG+GGG	+	chr2_4:11377799-11377818	None:intergenic	40.0%
	CAGATATCACTCAAAGTGCA+GGG	+	chr2_4:11377800-11377819	None:intergenic	40.0%
	ACTTTGAGTGATATCTGCAG+TGG	-	chr2_4:11377803-11377822	Msa0315170:intron	40.0%
!	ATTGGAGAGTTTGAGATCAG+TGG	+	chr2_4:11377862-11377881	Msa0315180:CDS	40.0%
	TATTTGGCAATGCATGGTCT+TGG	-	chr2_4:11377955-11377974	Msa0315170:exon	40.0%
	CAAATCGTTCGACCTGGAAA+CGG	-	chr2_4:11376245-11376264	Msa0315170:three_prime_UTR	45.0%
!!	TTTTGACCAGGAGTTGGCTT+AGG	+	chr2_4:11376272-11376291	None:intergenic	45.0%
!	AAAGTGAATGGATTCTGGCC+AGG	+	chr2_4:11376615-11376634	None:intergenic	45.0%
	GGCAAAGGTTCTGTCATCAA+AGG	-	chr2_4:11376642-11376661	Msa0315170:intron	45.0%
!!	GAGACTTTAGGACTTACGAG+TGG	+	chr2_4:11376788-11376807	None:intergenic	45.0%
	TTGTGTGTAGGATGGGATGA+AGG	-	chr2_4:11376995-11377014	Msa0315170:intron	45.0%
!!	GGTGTCATTGGCATGCAAAT+CGG	-	chr2_4:11377016-11377035	Msa0315170:intron	45.0%
	AAGGTCATGTTTGGATGTGC+AGG	+	chr2_4:11377362-11377381	None:intergenic	45.0%
	ATACACGCGACTCGATTAAG+TGG	+	chr2_4:11377441-11377460	None:intergenic	45.0%
	CTAGCGCCATAAGCATAATC+AGG	+	chr2_4:11377624-11377643	None:intergenic	45.0%
	GATTATGCTTATGGCGCTAG+TGG	-	chr2_4:11377624-11377643	Msa0315170:intron	45.0%
	GCAGATATCACTCAAAGTGC+AGG	+	chr2_4:11377801-11377820	None:intergenic	45.0%
	CAAATCGTTCGACCTGGAAA+CGG	-	chr2_4:11376245-11376264	Msa0315170:three_prime_UTR	45.0%
!!	TTTTGACCAGGAGTTGGCTT+AGG	+	chr2_4:11376272-11376291	None:intergenic	45.0%
!	AAAGTGAATGGATTCTGGCC+AGG	+	chr2_4:11376615-11376634	None:intergenic	45.0%
	GGCAAAGGTTCTGTCATCAA+AGG	-	chr2_4:11376642-11376661	Msa0315170:intron	45.0%
!!	GAGACTTTAGGACTTACGAG+TGG	+	chr2_4:11376788-11376807	None:intergenic	45.0%
	TTGTGTGTAGGATGGGATGA+AGG	-	chr2_4:11376995-11377014	Msa0315170:intron	45.0%
!!	GGTGTCATTGGCATGCAAAT+CGG	-	chr2_4:11377016-11377035	Msa0315170:intron	45.0%
	AAGGTCATGTTTGGATGTGC+AGG	+	chr2_4:11377362-11377381	None:intergenic	45.0%
	ATACACGCGACTCGATTAAG+TGG	+	chr2_4:11377441-11377460	None:intergenic	45.0%
	CTAGCGCCATAAGCATAATC+AGG	+	chr2_4:11377624-11377643	None:intergenic	45.0%
	GATTATGCTTATGGCGCTAG+TGG	-	chr2_4:11377624-11377643	Msa0315170:intron	45.0%
	GCAGATATCACTCAAAGTGC+AGG	+	chr2_4:11377801-11377820	None:intergenic	45.0%
!!	TAATTTAATTCAAATTATAA+GGG	+	chr2_4:11376385-11376404	None:intergenic	5.0%
!!	TAATTTAATTCAAATTATAA+GGG	+	chr2_4:11376385-11376404	None:intergenic	5.0%
	GAGAAGCAAATCGTTCGACC+TGG	-	chr2_4:11376239-11376258	Msa0315170:three_prime_UTR	50.0%
!!	GTGACGTTTTGACCAGGAGT+TGG	+	chr2_4:11376278-11376297	None:intergenic	50.0%
!!	TGGACAGTGACGTTTTGACC+AGG	+	chr2_4:11376284-11376303	None:intergenic	50.0%
!!	GGATGGGATGAAGGTGTCAT+TGG	-	chr2_4:11377004-11377023	Msa0315170:intron	50.0%
	TCGGAGAAGTTGCTCGTCTT+CGG	-	chr2_4:11377035-11377054	Msa0315170:intron	50.0%
!	TTGGGTTGTATTCCCCAAGC+AGG	+	chr2_4:11377654-11377673	None:intergenic	50.0%
	GAGAAGCAAATCGTTCGACC+TGG	-	chr2_4:11376239-11376258	Msa0315170:three_prime_UTR	50.0%
!!	GTGACGTTTTGACCAGGAGT+TGG	+	chr2_4:11376278-11376297	None:intergenic	50.0%
!!	TGGACAGTGACGTTTTGACC+AGG	+	chr2_4:11376284-11376303	None:intergenic	50.0%
!!	GGATGGGATGAAGGTGTCAT+TGG	-	chr2_4:11377004-11377023	Msa0315170:intron	50.0%
	TCGGAGAAGTTGCTCGTCTT+CGG	-	chr2_4:11377035-11377054	Msa0315170:intron	50.0%
!	TTGGGTTGTATTCCCCAAGC+AGG	+	chr2_4:11377654-11377673	None:intergenic	50.0%
!	GTTGGCTTAGGACCGTTTCC+AGG	+	chr2_4:11376260-11376279	None:intergenic	55.0%
	AACGGTCCTAAGCCAACTCC+TGG	-	chr2_4:11376263-11376282	Msa0315170:three_prime_UTR	55.0%
	AACGTCACTGTCCACTGCAC+CGG	-	chr2_4:11376290-11376309	Msa0315170:three_prime_UTR	55.0%
	ACTGTCCACTGCACCGGATA+CGG	-	chr2_4:11376296-11376315	Msa0315170:three_prime_UTR	55.0%
!	GAGAGTGTGCTCACCGTATC+CGG	+	chr2_4:11376312-11376331	None:intergenic	55.0%
	CGGAGAAGTTGCTCGTCTTC+GGG	-	chr2_4:11377036-11377055	Msa0315170:intron	55.0%
	CGCTAGTGGCTTTCCTGCTT+GGG	-	chr2_4:11377638-11377657	Msa0315170:intron	55.0%
	GCTAGTGGCTTTCCTGCTTG+GGG	-	chr2_4:11377639-11377658	Msa0315170:intron	55.0%
!	GTTGGCTTAGGACCGTTTCC+AGG	+	chr2_4:11376260-11376279	None:intergenic	55.0%
	AACGGTCCTAAGCCAACTCC+TGG	-	chr2_4:11376263-11376282	Msa0315170:three_prime_UTR	55.0%
	AACGTCACTGTCCACTGCAC+CGG	-	chr2_4:11376290-11376309	Msa0315170:three_prime_UTR	55.0%
	ACTGTCCACTGCACCGGATA+CGG	-	chr2_4:11376296-11376315	Msa0315170:three_prime_UTR	55.0%
!	GAGAGTGTGCTCACCGTATC+CGG	+	chr2_4:11376312-11376331	None:intergenic	55.0%
	CGGAGAAGTTGCTCGTCTTC+GGG	-	chr2_4:11377036-11377055	Msa0315170:intron	55.0%
	CGCTAGTGGCTTTCCTGCTT+GGG	-	chr2_4:11377638-11377657	Msa0315170:intron	55.0%
	GCTAGTGGCTTTCCTGCTTG+GGG	-	chr2_4:11377639-11377658	Msa0315170:intron	55.0%
	GCGCTAGTGGCTTTCCTGCT+TGG	-	chr2_4:11377637-11377656	Msa0315170:intron	60.0%
	GCGCTAGTGGCTTTCCTGCT+TGG	-	chr2_4:11377637-11377656	Msa0315170:intron	60.0%
	GCTCACCGTATCCGGTGCAG+TGG	+	chr2_4:11376304-11376323	None:intergenic	65.0%
	GCTCACCGTATCCGGTGCAG+TGG	+	chr2_4:11376304-11376323	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	11376135	11378050	11376135	ID=Msa0315170;Name=Msa0315170
chr2_4	mRNA	11376135	11378050	11376135	ID=Msa0315170-mRNA-1;Parent=Msa0315170;Name=Msa0315170-mRNA-1;_AED=0.01;_eAED=0.01;_QI=95\|1\|1\|1\|1\|1\|5\|334\|112
chr2_4	exon	11376135	11376570	11376135	ID=Msa0315170-mRNA-1:exon:4383;Parent=Msa0315170-mRNA-1
chr2_4	exon	11377128	11377180	11377128	ID=Msa0315170-mRNA-1:exon:4382;Parent=Msa0315170-mRNA-1
chr2_4	exon	11377522	11377583	11377522	ID=Msa0315170-mRNA-1:exon:4381;Parent=Msa0315170-mRNA-1
chr2_4	exon	11377670	11377703	11377670	ID=Msa0315170-mRNA-1:exon:4380;Parent=Msa0315170-mRNA-1
chr2_4	exon	11377868	11378050	11377868	ID=Msa0315170-mRNA-1:exon:4379;Parent=Msa0315170-mRNA-1
chr2_4	five_prime_UTR	11377956	11378050	11377956	ID=Msa0315170-mRNA-1:five_prime_utr;Parent=Msa0315170-mRNA-1
chr2_4	CDS	11377868	11377955	11377868	ID=Msa0315170-mRNA-1:cds;Parent=Msa0315170-mRNA-1
chr2_4	CDS	11377670	11377703	11377670	ID=Msa0315170-mRNA-1:cds;Parent=Msa0315170-mRNA-1
chr2_4	CDS	11377522	11377583	11377522	ID=Msa0315170-mRNA-1:cds;Parent=Msa0315170-mRNA-1
chr2_4	CDS	11377128	11377180	11377128	ID=Msa0315170-mRNA-1:cds;Parent=Msa0315170-mRNA-1
chr2_4	CDS	11376469	11376570	11376469	ID=Msa0315170-mRNA-1:cds;Parent=Msa0315170-mRNA-1
chr2_4	three_prime_UTR	11376135	11376468	11376135	ID=Msa0315170-mRNA-1:three_prime_utr;Parent=Msa0315170-mRNA-1

Gene Sequence	>Msa0315170 ATGGGAGTTGAGAAGCAAATCGTTCGACCTGGAAACGGTCCTAAGCCAACTCCTGGTCAAAACGTCACTGTCCACTGCACCGGATACGGCAAAAACGGTGACCTTTCTCAGAAATTCTGGAGTACAAAAGATCCTGGCCAGAATCCATTCACTTTCAAAATCGGCAAAGGTTCTGTCATCAAAGGATGGGATGAAGGTGTCATTGGCATGCAAATCGGAGAAGTTGCTCGTCTTCGGTGCTCTCCTGATTATGCTTATGGCGCTAGTGGCTTTCCTGCTTGGGGAATACAACCCAACTCTGTTTTGGAGTTTGAGATTGAGGTCTTGAGTGCAGAATGA
Protein sequence	>Msa0315170 MGVEKQIVRPGNGPKPTPGQNVTVHCTGYGKNGDLSQKFWSTKDPGQNPFTFKIGKGSVIKGWDEGVIGMQIGEVARLRCSPDYAYGASGFPAWGIQPNSVLEFEIEVLSAE*