AlfalfaGEDB | Msa0069450

M. sativa cultivar zhongmu-4 / Msa0069450

KEGG: -
GO: -
PFAM: PF03195.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0069450	XP_003591977.1	98.696	230	2	1	1	230	1	229	3.97e-162	457

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0069450	sp\|Q9LQR0\|LBD1_ARATH	68.072	166	44	5	69	230	29	189	2.86e-73	223

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0069450	G7I9N1	98.696	230	2	1	1	230	1	229	1.90e-162	457

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TFs/TRs:

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Gene ID	Type	Classification
Msa0069450	TF	LOB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0069450	Msa0131480	0.962902	1.963395e-121	-8.615850e-47
Msa0069450	Msa0131570	0.938493	6.146323e-99	-8.615850e-47
Msa0069450	Msa0177350	0.981746	2.397577e-153	-8.615850e-47
Msa0069450	Msa0581590	0.961107	2.547502e-119	-8.615850e-47
Msa0069450	Msa0660580	0.961265	1.675957e-119	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0069450	MtrunA17_Chr1g0200201	98.696	230	2	1	1	230	1	229	3.65e-166	457
Msa0069450	MtrunA17_Chr4g0030001	67.416	178	45	7	64	230	15	190	4.64e-77	230
Msa0069450	MtrunA17_Chr2g0311601	71.818	110	31	0	71	180	11	120	1.24e-54	172
Msa0069450	MtrunA17_Chr4g0043421	67.890	109	35	0	72	180	14	122	2.54e-51	164
Msa0069450	MtrunA17_Chr3g0114061	71.845	103	29	0	72	174	7	109	1.18e-50	162
Msa0069450	MtrunA17_Chr5g0437571	65.487	113	39	0	72	184	5	117	1.80e-50	162
Msa0069450	MtrunA17_Chr3g0106101	66.055	109	37	0	72	180	6	114	1.02e-49	160
Msa0069450	MtrunA17_Chr2g0326051	63.200	125	38	4	114	230	1	125	1.85e-44	145
Msa0069450	MtrunA17_Chr5g0438091	54.386	114	52	0	71	184	37	150	2.24e-41	139
Msa0069450	MtrunA17_Chr3g0117121	59.048	105	43	0	72	176	9	113	4.64e-41	139

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0069450	AT1G07900.1	68.072	166	44	5	69	230	29	189	2.90e-74	223
Msa0069450	AT2G28500.1	63.187	182	47	4	69	230	51	232	2.96e-71	217
Msa0069450	AT2G30130.1	68.519	108	34	0	72	179	7	114	3.60e-50	162
Msa0069450	AT1G16530.1	65.421	107	36	1	71	176	12	118	2.86e-47	154
Msa0069450	AT1G31320.1	67.273	110	36	0	72	181	12	121	2.26e-43	144
Msa0069450	AT5G63090.4	60.194	103	41	0	72	174	10	112	4.48e-41	139
Msa0069450	AT5G63090.3	60.194	103	41	0	72	174	10	112	4.48e-41	139
Msa0069450	AT5G63090.2	60.194	103	41	0	72	174	10	112	4.48e-41	139
Msa0069450	AT5G63090.1	60.194	103	41	0	72	174	10	112	4.48e-41	139
Msa0069450	AT2G23660.2	58.654	104	43	0	72	175	4	107	6.48e-41	142

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Find 72 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGTGTGGGAGATTGTAA+AGG	0.533363	1_2:+37888770	None:intergenic
AACTCATATCAACATGAGGT+TGG	0.513338	1_2:+37887401	None:intergenic
AACTTGAGTGGATCAGTTGG+AGG	0.595286	1_2:+37888602	None:intergenic
AAGCAACTCATATCAACATG+AGG	0.663119	1_2:+37887397	None:intergenic
AATATTGAGAAGCTGGTGAA+GGG	0.602247	1_2:+37888747	None:intergenic
AATTAATTTCATGTCCAAAG+AGG	0.542951	1_2:+37887340	None:intergenic
AATTTCCAAACTCCAAGTGG+AGG	0.632065	1_2:-37887208	Msa0069450:three_prime_UTR
ACCATTGCTCATAGAGTCTT+TGG	0.230237	1_2:-37888578	Msa0069450:CDS
ACTCATATCAACATGAGGTT+GGG	0.426563	1_2:+37887402	None:intergenic
AGAAACAGAGGAAAACAGAG+AGG	0.690643	1_2:+37888918	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGGATGGAAAAGATAACAAA+AGG	-	chr1_2:37887885-37887904	Msa0069450:intron	30.0%
AAGATATAGTGGTTACACTA+TGG	+	chr1_2:37888125-37888144	None:intergenic	30.0%
GGTAGTTCCTGAAAAATTAA+TGG	+	chr1_2:37888466-37888485	None:intergenic	30.0%
TGGATGGAAAAGATAACAAA+AGG	-	chr1_2:37887885-37887904	Msa0069450:intron	30.0%
AAGATATAGTGGTTACACTA+TGG	+	chr1_2:37888125-37888144	None:intergenic	30.0%
GGTAGTTCCTGAAAAATTAA+TGG	+	chr1_2:37888466-37888485	None:intergenic	30.0%
TTGTGTTATGTGAGAAACAG+AGG	+	chr1_2:37887236-37887255	None:intergenic	35.0%
TGTGAATGGGAACATGAATT+GGG	+	chr1_2:37887305-37887324	None:intergenic	35.0%
AATATTGAGAAGCTGGTGAA+GGG	+	chr1_2:37887395-37887414	None:intergenic	35.0%
CAACATCATTAAGTTCTTGC+AGG	-	chr1_2:37887590-37887609	Msa0069450:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	37887182	37888979	37887182	ID=Msa0069450;Name=Msa0069450
chr1_2	mRNA	37887182	37888979	37887182	ID=Msa0069450-mRNA-1;Parent=Msa0069450;Name=Msa0069450-mRNA-1;_AED=0.03;_eAED=0.03;_QI=73\|1\|1\|1\|1\|1\|2\|166\|230
chr1_2	exon	37887182	37887683	37887182	ID=Msa0069450-mRNA-1:exon:11957;Parent=Msa0069450-mRNA-1
chr1_2	exon	37888550	37888979	37888550	ID=Msa0069450-mRNA-1:exon:11956;Parent=Msa0069450-mRNA-1
chr1_2	five_prime_UTR	37888907	37888979	37888907	ID=Msa0069450-mRNA-1:five_prime_utr;Parent=Msa0069450-mRNA-1
chr1_2	CDS	37888550	37888906	37888550	ID=Msa0069450-mRNA-1:cds;Parent=Msa0069450-mRNA-1
chr1_2	CDS	37887348	37887683	37887348	ID=Msa0069450-mRNA-1:cds;Parent=Msa0069450-mRNA-1
chr1_2	three_prime_UTR	37887182	37887347	37887182	ID=Msa0069450-mRNA-1:three_prime_utr;Parent=Msa0069450-mRNA-1

Gene Sequence

>Msa0069450

ATGGAACACAAAATGAAAACCACTACTTCTCCTCCTCATCTACCAATCCCAATTCATGTTCCCATTCACACCATGCTATCAAACTCTACTACACCATCTTCATCTTCTTCACCTCCTTTACAATCTCCCACACCTTTCCCTTCACCAGCTTCTCAATATTCTCCTCCCTCGGCCGCTCCTCAACCACCATCTCCTCCACCACCAATTATTGTTAGCCCTTGTGCTGCCTGCAAGATTCTCCGTCGCAGGTGTGTTGAAAAGTGTGTGTTAGCTCCGTATTTTCCTCCAACTGATCCACTCAAGTTCACCATTGCTCATAGAGTCTTTGGAGCTAGCAACATCATTAAGTTCTTGCAGGAACTACCTGAACCTCAGAGAGCAGATGCAGTAAGCAGCATGGTTTATGAAGCAAATGCTAGGATAAGGGACCCAGTTTATGGGTGTGCAGGTGCAATATGTCAACTTCAAAAACAAGTTAGTGAGTTACAAACACAGCTAGCCAAAGCACAAGCTGAGCTAGTGAACATGCATTGCCAACAAGCAAATTTAGTGGCACTAATTTGCATGGAAATGAGTACTCAGTCATCACCAGACCATGACATGATGATTAATCAGCCCCAACCTCATGTTGATATGAGTTGCTTTTTAGAGGACAACAATTTTGGGTCTGCTTGGGAGCCTCTTTGGACATGA

Protein sequence

>Msa0069450

MEHKMKTTTSPPHLPIPIHVPIHTMLSNSTTPSSSSSPPLQSPTPFPSPASQYSPPSAAPQPPSPPPPIIVSPCAACKILRRRCVEKCVLAPYFPPTDPLKFTIAHRVFGASNIIKFLQELPEPQRADAVSSMVYEANARIRDPVYGCAGAICQLQKQVSELQTQLAKAQAELVNMHCQQANLVALICMEMSTQSSPDHDMMINQPQPHVDMSCFLEDNNFGSAWEPLWT*