AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0077690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0077690 MtrunA17_Chr1g0187771 99.320 147 1 0 1 147 1 147 1.16e-108 305
Msa0077690 MtrunA17_Chr7g0261251 78.912 147 30 1 1 147 1 146 1.76e-86 249
Msa0077690 MtrunA17_Chr1g0182501 62.319 138 52 0 10 147 2 139 1.44e-66 198
Msa0077690 MtrunA17_Chr4g0037251 61.644 146 50 1 1 146 3 142 2.99e-66 197
Msa0077690 MtrunA17_Chr5g0397641 61.314 137 51 1 10 146 2 136 1.21e-63 191
Msa0077690 MtrunA17_Chr8g0386651 60.584 137 52 1 10 146 2 136 1.17e-61 186
Msa0077690 MtrunA17_Chr1g0203211 57.447 141 58 1 6 146 22 160 5.36e-60 182
Msa0077690 MtrunA17_Chr2g0291571 55.797 138 61 0 10 147 2 139 9.00e-59 179
Msa0077690 MtrunA17_Chr8g0380291 55.072 138 62 0 10 147 2 139 5.26e-56 172
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0077690 AT2G31200.1 76.190 147 34 1 1 147 1 146 6.69e-82 238
Msa0077690 AT5G59890.1 61.594 138 53 0 10 147 2 139 3.00e-65 195
Msa0077690 AT3G46010.2 57.931 145 61 0 3 147 6 150 8.65e-65 194
Msa0077690 AT4G00680.2 58.042 143 60 0 5 147 7 149 1.33e-64 194
Msa0077690 AT3G46010.1 60.145 138 55 0 10 147 2 139 2.43e-64 193
Msa0077690 AT4G00680.1 58.696 138 57 0 10 147 2 139 5.13e-64 192
Msa0077690 AT1G01750.1 58.696 138 57 0 10 147 2 139 1.95e-63 191
Msa0077690 AT1G01750.2 58.696 138 57 0 10 147 7 144 2.17e-63 191
Msa0077690 AT2G16700.1 58.503 147 55 1 1 147 3 143 3.05e-63 190
Msa0077690 AT4G25590.1 62.774 137 49 1 10 146 2 136 3.65e-63 190
Msa0077690 AT3G46000.1 60.870 138 52 1 10 147 2 137 2.68e-62 188
Msa0077690 AT3G46000.2 60.870 138 52 1 10 147 21 156 3.76e-62 188
Msa0077690 AT5G59890.3 61.364 132 51 0 16 147 1 132 2.15e-61 185
Msa0077690 AT5G59890.2 61.364 132 51 0 16 147 1 132 2.15e-61 185
Msa0077690 AT2G16700.4 61.364 132 51 0 16 147 1 132 1.13e-60 183
Msa0077690 AT2G16700.2 61.364 132 51 0 16 147 1 132 1.13e-60 183
Msa0077690 AT4G34970.1 58.394 137 57 0 11 147 5 141 2.00e-60 183
Msa0077690 AT5G52360.1 61.314 137 51 1 10 146 2 136 4.61e-60 182
Msa0077690 AT4G25590.2 62.595 131 47 1 16 146 1 129 1.72e-59 181
Msa0077690 AT5G59880.1 55.797 138 61 0 10 147 2 139 2.80e-59 180
Msa0077690 AT3G46000.3 60.606 132 50 1 16 147 1 130 1.34e-58 178
Msa0077690 AT5G59880.2 48.551 138 56 1 10 147 2 124 3.51e-46 146
Msa0077690 AT2G16700.3 50.427 117 51 2 1 117 3 112 9.61e-37 122
Msa0077690 AT3G45990.1 42.754 138 68 3 16 147 1 133 2.41e-34 117

Find 61 sgRNAs with CRISPR-Local

Find 273 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCCTTAAGTACTATTATTA+TGG 0.209108 1_2:-54655751 Msa0077690:three_prime_UTR
TTGTAGGGAAATGCATCTTC+TGG 0.261866 1_2:-54657932 Msa0077690:intron
GCAGAGCGTAAGCACATTTC+AGG 0.286465 1_2:-54657894 Msa0077690:CDS
AACCATAATAATAGTACTTA+AGG 0.320026 1_2:+54655749 None:intergenic
TGTGAAGGCTGATACTGATA+TGG 0.329087 1_2:-54655669 Msa0077690:three_prime_UTR
AGAATTGTGTGGAGATGAAA+AGG 0.335598 1_2:+54659385 None:intergenic
AAGGGTTATGTCTATTAGTT+AGG 0.342272 1_2:-54655808 Msa0077690:three_prime_UTR
TGAAGAGTCAAGAATTTAGA+AGG 0.351710 1_2:+54659331 None:intergenic
GGGAATCCACTATGAGATTC+AGG 0.356002 1_2:-54655901 Msa0077690:CDS
CCTATGCAGAGGGGTCTCTT+AGG 0.385406 1_2:+54655639 None:intergenic
AGAACCATCATATGGCAATA+AGG 0.388005 1_2:+54655603 None:intergenic
CAGGTTCAGAAGGGAGCTGC+AGG 0.400251 1_2:-54655922 Msa0077690:CDS
GCTTTCAGAGTGGGAGTTGG+AGG 0.419649 1_2:-54659283 Msa0077690:CDS
GTGATTAGTTCGCACGCTCC+TGG 0.421098 1_2:+54655844 None:intergenic
TGTTCCTTATTGCCATATGA+TGG 0.447267 1_2:-54655607 Msa0077690:three_prime_UTR
ACCATAATAATAGTACTTAA+GGG 0.452507 1_2:+54655750 None:intergenic
GATGTAGCATAAAGCATCTT+CGG 0.454388 1_2:+54655951 None:intergenic
ACTTCGAGTTCCATCTCTGT+TGG 0.461999 1_2:+54655870 None:intergenic
ATTGCCATATGATGGTTCTC+AGG 0.462653 1_2:-54655599 Msa0077690:three_prime_UTR
CTTCGAGTTCCATCTCTGTT+GGG 0.469550 1_2:+54655871 None:intergenic
ATAACCCTTTCAGTTTGCTC+AGG 0.490136 1_2:+54655822 None:intergenic
TGTAGGGAAATGCATCTTCT+GGG 0.504974 1_2:-54657931 Msa0077690:intron
GGATATTGAAACTCTGTCAA+TGG 0.511931 1_2:-54655553 Msa0077690:three_prime_UTR
CTTTCAGAGTGGGAGTTGGA+GGG 0.516133 1_2:-54659282 Msa0077690:CDS
ATCACCTGAGCAAACTGAAA+GGG 0.520857 1_2:-54655826 Msa0077690:three_prime_UTR
TAAATAACATTCACCTGTGA+AGG 0.522452 1_2:-54655684 Msa0077690:three_prime_UTR
TCCACCTGAGAACCATCATA+TGG 0.526072 1_2:+54655595 None:intergenic
ATGGCTTTCAGAGTGGGAGT+TGG 0.530306 1_2:-54659286 Msa0077690:CDS
TTTCAGAGTGGGAGTTGGAG+GGG 0.532929 1_2:-54659281 Msa0077690:intron
ATAGCTATGGCTTTCAGAGT+GGG 0.535262 1_2:-54659292 Msa0077690:exon
CAGCAGCAGCTTCATAGCTA+TGG 0.536263 1_2:-54659305 Msa0077690:exon
GGGAAATGCATCTTCTGGGA+TGG 0.538512 1_2:-54657927 Msa0077690:CDS
CATCAAAGGACAGGTTCAGA+AGG 0.548452 1_2:-54655932 Msa0077690:CDS
TTGTAGAGAGAGAAAGTGAG+AGG 0.554165 1_2:+54659356 None:intergenic
GGAAATGCATCTTCTGGGAT+GGG 0.555903 1_2:-54657926 Msa0077690:CDS
GGCAACAGACCCAACAGAGA+TGG 0.557284 1_2:-54655880 Msa0077690:CDS
AGCTAAAATTCCTATGCAGA+GGG 0.559914 1_2:+54655629 None:intergenic
GATGGAACTCGAAGTCCTCC+AGG 0.560122 1_2:-54655862 Msa0077690:CDS
AAATCATCATAGCTCTCAGA+AGG 0.561770 1_2:+54657785 None:intergenic
GTGAAGGCTGATACTGATAT+GGG 0.562558 1_2:-54655668 Msa0077690:three_prime_UTR
GGACGAATACGAGCTACAGA+AGG 0.568172 1_2:+54655972 None:intergenic
CCTAAGAGACCCCTCTGCAT+AGG 0.568605 1_2:-54655639 Msa0077690:three_prime_UTR
CATAGCTATGGCTTTCAGAG+TGG 0.573358 1_2:-54659293 Msa0077690:exon
GAATTGTGTGGAGATGAAAA+GGG 0.573731 1_2:+54659386 None:intergenic
AGAGGAGAGAGAGAATTGTG+TGG 0.574529 1_2:+54659374 None:intergenic
CTGTTGCCTGAATCTCATAG+TGG 0.585469 1_2:+54655895 None:intergenic
TTTATGCTACATCAAAGGAC+AGG 0.592719 1_2:-54655941 Msa0077690:CDS
ATATCAGTATCAGCCTTCAC+AGG 0.593659 1_2:+54655671 None:intergenic
GATGCTTTATGCTACATCAA+AGG 0.601779 1_2:-54655946 Msa0077690:CDS
ACTCTGTCAATGGAGGTGTT+TGG 0.602656 1_2:-54655543 Msa0077690:three_prime_UTR
AATCACCTGAGCAAACTGAA+AGG 0.609728 1_2:-54655827 Msa0077690:three_prime_UTR
TTTGTTGAAAACATTTCTTG+AGG 0.612163 1_2:+54655775 None:intergenic
CAGCTAAAATTCCTATGCAG+AGG 0.616100 1_2:+54655628 None:intergenic
ATCAAAGGACAGGTTCAGAA+GGG 0.621100 1_2:-54655931 Msa0077690:CDS
GCCATATGATGGTTCTCAGG+TGG 0.634844 1_2:-54655596 Msa0077690:three_prime_UTR
GCACATTTCAGGAATTGCAG+AGG 0.661602 1_2:-54657883 Msa0077690:CDS
ATTAGTTCGCACGCTCCTGG+AGG 0.663424 1_2:+54655847 None:intergenic
AGGTTCAGAAGGGAGCTGCA+GGG 0.689968 1_2:-54655921 Msa0077690:CDS
TATTGAAACTCTGTCAATGG+AGG 0.714240 1_2:-54655550 Msa0077690:three_prime_UTR
GCTAAAATTCCTATGCAGAG+GGG 0.724256 1_2:+54655630 None:intergenic
AATCATCATAGCTCTCAGAA+GGG 0.724970 1_2:+54657786 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGGTTAAAAATTATTTT+AGG + chr1_2:54655496-54655515 None:intergenic 10.0%
!!! TTAATTAGTTTATATATTGT+AGG - chr1_2:54658571-54658590 Msa0077690:intron 10.0%
!! AAACATGATTGAAAAATATA+TGG + chr1_2:54655899-54655918 None:intergenic 15.0%
!!! ATTTGAATAAAATTTTCTGA+GGG - chr1_2:54656085-54656104 Msa0077690:intron 15.0%
!!! TAAAAAATGCTTTTGATATT+TGG - chr1_2:54657842-54657861 Msa0077690:CDS 15.0%
!! AACACAAAAATGAATAAAAA+AGG + chr1_2:54658533-54658552 None:intergenic 15.0%
!! AAAAAATGATAGAATGAAGA+TGG + chr1_2:54655690-54655709 None:intergenic 20.0%
!!! CATTTGAATAAAATTTTCTG+AGG - chr1_2:54656084-54656103 Msa0077690:intron 20.0%
!! ACTTAATAATCATATCAAAG+GGG + chr1_2:54656430-54656449 None:intergenic 20.0%
!! CACTTAATAATCATATCAAA+GGG + chr1_2:54656431-54656450 None:intergenic 20.0%
!! ACACTTAATAATCATATCAA+AGG + chr1_2:54656432-54656451 None:intergenic 20.0%
!! TTGATATGATTATTAAGTGT+TGG - chr1_2:54656432-54656451 Msa0077690:intron 20.0%
!! TGATATGATTATTAAGTGTT+GGG - chr1_2:54656433-54656452 Msa0077690:intron 20.0%
!!! GATTATTTTGGTTTGAAAAA+AGG - chr1_2:54656950-54656969 Msa0077690:intron 20.0%
!!! TAAAAAAGATTTTGCTCTTT+TGG + chr1_2:54657251-54657270 None:intergenic 20.0%
!! ACATATGTATATATTGCATT+TGG - chr1_2:54657366-54657385 Msa0077690:intron 20.0%
!!! AAATAAAAAACCCTTTTAGA+AGG + chr1_2:54657913-54657932 None:intergenic 20.0%
!! AGACAAATATAATCAAGTAT+TGG + chr1_2:54658020-54658039 None:intergenic 20.0%
!! ATACTTGATTATATTTGTCT+CGG - chr1_2:54658020-54658039 Msa0077690:intron 20.0%
!! AATAATTCATATGAATGGAA+AGG + chr1_2:54658074-54658093 None:intergenic 20.0%
!! ATCACAATAATTCATATGAA+TGG + chr1_2:54658079-54658098 None:intergenic 20.0%
!! TTCATATGAATTATTGTGAT+AGG - chr1_2:54658079-54658098 Msa0077690:intron 20.0%
!!! TTTTGTGTTGATTTTTCTTT+TGG - chr1_2:54658544-54658563 Msa0077690:intron 20.0%
!!! TTTGTGTTGATTTTTCTTTT+GGG - chr1_2:54658545-54658564 Msa0077690:intron 20.0%
!! ATTTATATACATCAGAATCA+TGG - chr1_2:54658627-54658646 Msa0077690:intron 20.0%
!! GACAATCTTAAAAAAAACTT+TGG - chr1_2:54658660-54658679 Msa0077690:intron 20.0%
!!! TTTTATTTTCTGCTTACATA+TGG - chr1_2:54658795-54658814 Msa0077690:intron 20.0%
!! ACCATAATAATAGTACTTAA+GGG + chr1_2:54659191-54659210 None:intergenic 20.0%
!! AACCATAATAATAGTACTTA+AGG + chr1_2:54659192-54659211 None:intergenic 20.0%
!!! GAAGTAATATGCTTATTTTA+TGG - chr1_2:54659364-54659383 Msa0077690:five_prime_UTR 20.0%
!!! TTGTTTTTGTCGGATTTTAT+TGG - chr1_2:54655764-54655783 Msa0077690:three_prime_UTR 25.0%
!!! TCAGTTTTAATTTTGCTTTG+TGG - chr1_2:54655832-54655851 Msa0077690:exon 25.0%
!! AAACATATATCTTCGCATTT+TGG + chr1_2:54656164-54656183 None:intergenic 25.0%
! TCCAATGATGTTAAGAAATT+AGG - chr1_2:54656193-54656212 Msa0077690:intron 25.0%
! CATAGAAAGAATGCAAAAAT+GGG + chr1_2:54656306-54656325 None:intergenic 25.0%
! AAGTTAAATGTAGATAGTGT+AGG - chr1_2:54656525-54656544 Msa0077690:intron 25.0%
! GTATAAAGTGAGAAAATCAA+GGG + chr1_2:54656584-54656603 None:intergenic 25.0%
! AGTATAAAGTGAGAAAATCA+AGG + chr1_2:54656585-54656604 None:intergenic 25.0%
! ACTAAATCACCAATCAAAAA+GGG + chr1_2:54656776-54656795 None:intergenic 25.0%
! AACTAAATCACCAATCAAAA+AGG + chr1_2:54656777-54656796 None:intergenic 25.0%
!!! AGGACTTTAGTATTCTTTTT+TGG - chr1_2:54656920-54656939 Msa0077690:intron 25.0%
!!! GGACTTTAGTATTCTTTTTT+GGG - chr1_2:54656921-54656940 Msa0077690:intron 25.0%
!!! GACTTTAGTATTCTTTTTTG+GGG - chr1_2:54656922-54656941 Msa0077690:intron 25.0%
!!! ACTTTAGTATTCTTTTTTGG+GGG - chr1_2:54656923-54656942 Msa0077690:intron 25.0%
!!! TTTTGGTTTGAAAAAAGGAT+TGG - chr1_2:54656955-54656974 Msa0077690:intron 25.0%
! AATTTCGTGTAACTAATATG+TGG - chr1_2:54656978-54656997 Msa0077690:intron 25.0%
!!! TAATATGTGGATTTGTTGTA+GGG - chr1_2:54656991-54657010 Msa0077690:intron 25.0%
! AATTGATGAGAAAAAGAAAG+AGG - chr1_2:54657107-54657126 Msa0077690:intron 25.0%
! AGCATATTGCTAATTTACTA+GGG - chr1_2:54657644-54657663 Msa0077690:intron 25.0%
!!! CTGGAATTTGAAATGATTTT+TGG + chr1_2:54657814-54657833 None:intergenic 25.0%
! AAACAACAATCACATGAATT+TGG + chr1_2:54658049-54658068 None:intergenic 25.0%
! TGATAAACTAATTGCCTATT+TGG + chr1_2:54658125-54658144 None:intergenic 25.0%
!!! TCTGATGTATTATTTCTGTT+TGG - chr1_2:54658296-54658315 Msa0077690:intron 25.0%
! CAATTGCAGAATATTATTGA+TGG + chr1_2:54658405-54658424 None:intergenic 25.0%
! ATACAGAATTAACAGTGATT+TGG + chr1_2:54658440-54658459 None:intergenic 25.0%
! TAATTCTGTATGTTTGGTAT+CGG - chr1_2:54658449-54658468 Msa0077690:intron 25.0%
!!! ATTTTTTTGCAATCCACTAT+TGG + chr1_2:54658702-54658721 None:intergenic 25.0%
! ATTATAGTATTGTAGCGTAA+CGG + chr1_2:54658742-54658761 None:intergenic 25.0%
! AGTAAACTCAAGCAAAATAA+AGG + chr1_2:54658922-54658941 None:intergenic 25.0%
!! ATTTTGCTTGAGTTTACTAA+TGG - chr1_2:54658924-54658943 Msa0077690:intron 25.0%
! TTTGTTGAAAACATTTCTTG+AGG + chr1_2:54659166-54659185 None:intergenic 25.0%
!! CATTTTTGCGAATTTAGCTT+GGG - chr1_2:54655511-54655530 Msa0077690:three_prime_UTR 30.0%
!!! ATTTTTGCGAATTTAGCTTG+GGG - chr1_2:54655512-54655531 Msa0077690:three_prime_UTR 30.0%
TGAAGAGTCAAGAATTTAGA+AGG + chr1_2:54655610-54655629 None:intergenic 30.0%
AAATCAAACCAAAATCAGCA+TGG + chr1_2:54655732-54655751 None:intergenic 30.0%
!!! TCGAATGTTGTTGTTTTTGT+CGG - chr1_2:54655754-54655773 Msa0077690:three_prime_UTR 30.0%
! TTCTTCCCACTACAAATTTT+GGG - chr1_2:54655807-54655826 Msa0077690:three_prime_UTR 30.0%
AAGAAAAACCGCATAATATG+AGG + chr1_2:54655864-54655883 None:intergenic 30.0%
GCCTAATTTCTTAACATCAT+TGG + chr1_2:54656197-54656216 None:intergenic 30.0%
TTGTCAAATGAAATCTTGTG+CGG - chr1_2:54656223-54656242 Msa0077690:intron 30.0%
! TTGAGTTACAAGTAGCTTTA+AGG - chr1_2:54656277-54656296 Msa0077690:intron 30.0%
CCATAGAAAGAATGCAAAAA+TGG + chr1_2:54656307-54656326 None:intergenic 30.0%
!! CCATTTTTGCATTCTTTCTA+TGG - chr1_2:54656304-54656323 Msa0077690:intron 30.0%
!!! TTATACTCTAGTCACTTTTG+AGG - chr1_2:54656598-54656617 Msa0077690:intron 30.0%
AGAAGTTTATGACTACACTT+CGG + chr1_2:54656632-54656651 None:intergenic 30.0%
ATGAGATATATCTTACTCCA+GGG + chr1_2:54656730-54656749 None:intergenic 30.0%
CTAAATCACCAATCAAAAAG+GGG + chr1_2:54656775-54656794 None:intergenic 30.0%
!! CTAATATGTGGATTTGTTGT+AGG - chr1_2:54656990-54657009 Msa0077690:intron 30.0%
! GCATATCTACAATCATTTTC+AGG + chr1_2:54657192-54657211 None:intergenic 30.0%
!!! GCAAAATCTTTTTTATCGCA+TGG - chr1_2:54657256-54657275 Msa0077690:intron 30.0%
! TTGCTCAATTTTTCTTGCAT+TGG - chr1_2:54657324-54657343 Msa0077690:intron 30.0%
ATGCCTATTCTTAATCTTCA+TGG - chr1_2:54657556-54657575 Msa0077690:intron 30.0%
TGCCTATTCTTAATCTTCAT+GGG - chr1_2:54657557-54657576 Msa0077690:intron 30.0%
TATTCTTAATCTTCATGGGT+GGG - chr1_2:54657561-54657580 Msa0077690:intron 30.0%
GAGCATATTGCTAATTTACT+AGG - chr1_2:54657643-54657662 Msa0077690:intron 30.0%
!! CTGTATTTTTGAATGGAGTA+GGG - chr1_2:54657678-54657697 Msa0077690:intron 30.0%
!! TGTATTTTTGAATGGAGTAG+GGG - chr1_2:54657679-54657698 Msa0077690:intron 30.0%
!!! AGCATTTTTTATCAAGAGTC+TGG + chr1_2:54657833-54657852 None:intergenic 30.0%
! TGCTTTTGATATTTGGCAAA+AGG - chr1_2:54657849-54657868 Msa0077690:CDS 30.0%
AACCAGATACTAAAAGAAAG+GGG + chr1_2:54657982-54658001 None:intergenic 30.0%
CAACCAGATACTAAAAGAAA+GGG + chr1_2:54657983-54658002 None:intergenic 30.0%
ACAACCAGATACTAAAAGAA+AGG + chr1_2:54657984-54658003 None:intergenic 30.0%
TGTTAGAAGAATGTAGGAAA+CGG - chr1_2:54658174-54658193 Msa0077690:intron 30.0%
CACTGTTAATTCTGTATGTT+TGG - chr1_2:54658443-54658462 Msa0077690:intron 30.0%
TTTATATATTGTAGGCATCC+TGG - chr1_2:54658579-54658598 Msa0077690:intron 30.0%
!! AGTGGATTGCAAAAAAATAG+CGG - chr1_2:54658704-54658723 Msa0077690:intron 30.0%
! CTAATGGATAATTGCTTTAC+AGG - chr1_2:54658940-54658959 Msa0077690:intron 30.0%
AAGGGTTATGTCTATTAGTT+AGG - chr1_2:54659130-54659149 Msa0077690:intron 30.0%
GCCCTTAAGTACTATTATTA+TGG - chr1_2:54659187-54659206 Msa0077690:intron 30.0%
TAAATAACATTCACCTGTGA+AGG - chr1_2:54659254-54659273 Msa0077690:intron 30.0%
GAACTAGAAAAATACGAAAC+AGG + chr1_2:54659437-54659456 None:intergenic 30.0%
CCAAGCTAAATTCGCAAAAA+TGG + chr1_2:54655513-54655532 None:intergenic 35.0%
!! CCATTTTTGCGAATTTAGCT+TGG - chr1_2:54655510-54655529 Msa0077690:three_prime_UTR 35.0%
!!! TTTTTGCGAATTTAGCTTGG+GGG - chr1_2:54655513-54655532 Msa0077690:three_prime_UTR 35.0%
GAATTGTGTGGAGATGAAAA+GGG + chr1_2:54655555-54655574 None:intergenic 35.0%
AGAATTGTGTGGAGATGAAA+AGG + chr1_2:54655556-54655575 None:intergenic 35.0%
! GTTCTTCCCACTACAAATTT+TGG - chr1_2:54655806-54655825 Msa0077690:three_prime_UTR 35.0%
TGAGACCCAAAATTTGTAGT+GGG + chr1_2:54655815-54655834 None:intergenic 35.0%
! GGTTGATTCCTCATATTATG+CGG - chr1_2:54655853-54655872 Msa0077690:CDS 35.0%
GCATGTCTTTAGTACTTTCT+TGG - chr1_2:54656107-54656126 Msa0077690:intron 35.0%
TCTTTAGTACTTTCTTGGAC+AGG - chr1_2:54656112-54656131 Msa0077690:intron 35.0%
!! GATTATTAAGTGTTGGGATC+CGG - chr1_2:54656439-54656458 Msa0077690:intron 35.0%
TTATGACTACACTTCGGATT+TGG + chr1_2:54656626-54656645 None:intergenic 35.0%
CATGAGATATATCTTACTCC+AGG + chr1_2:54656731-54656750 None:intergenic 35.0%
! TACAGTTTCCCCTTTTTGAT+TGG - chr1_2:54656764-54656783 Msa0077690:intron 35.0%
AATGAGGAAAACAGCATGTT+TGG + chr1_2:54656879-54656898 None:intergenic 35.0%
CTGGGATTCATTTCATTGAA+AGG - chr1_2:54656900-54656919 Msa0077690:intron 35.0%
!! TTTGGGGGAGTTGATTATTT+TGG - chr1_2:54656938-54656957 Msa0077690:intron 35.0%
TGAGAAAAAGAAAGAGGTTG+TGG - chr1_2:54657113-54657132 Msa0077690:intron 35.0%
AATCATCATAGCTCTCAGAA+GGG + chr1_2:54657155-54657174 None:intergenic 35.0%
AAATCATCATAGCTCTCAGA+AGG + chr1_2:54657156-54657175 None:intergenic 35.0%
!! TCTACAATCATTTTCAGGCA+AGG + chr1_2:54657187-54657206 None:intergenic 35.0%
AAAGACAGAAATCTGAACGA+GGG - chr1_2:54657443-54657462 Msa0077690:intron 35.0%
CACCCATGAAGATTAAGAAT+AGG + chr1_2:54657562-54657581 None:intergenic 35.0%
CTATTCTTAATCTTCATGGG+TGG - chr1_2:54657560-54657579 Msa0077690:intron 35.0%
!! TGCCAAGCTGTATTTTTGAA+TGG - chr1_2:54657671-54657690 Msa0077690:intron 35.0%
CTCCATTCAAAAATACAGCT+TGG + chr1_2:54657676-54657695 None:intergenic 35.0%
!! GCTGTATTTTTGAATGGAGT+AGG - chr1_2:54657677-54657696 Msa0077690:intron 35.0%
ACTATAGTACCTTGTTGAAC+TGG - chr1_2:54657759-54657778 Msa0077690:CDS 35.0%
CTATAGTACCTTGTTGAACT+GGG - chr1_2:54657760-54657779 Msa0077690:CDS 35.0%
TATAGTACCTTGTTGAACTG+GGG - chr1_2:54657761-54657780 Msa0077690:CDS 35.0%
GTTTATGCAAACGGTTCAAA+CGG + chr1_2:54657884-54657903 None:intergenic 35.0%
TAAACCCATAGCCTTCTAAA+AGG - chr1_2:54657899-54657918 Msa0077690:CDS 35.0%
! AAACCCATAGCCTTCTAAAA+GGG - chr1_2:54657900-54657919 Msa0077690:CDS 35.0%
!! AAACCCTTTTAGAAGGCTAT+GGG + chr1_2:54657906-54657925 None:intergenic 35.0%
!! AAAACCCTTTTAGAAGGCTA+TGG + chr1_2:54657907-54657926 None:intergenic 35.0%
! ACAACCTTGCTATAATCAAC+TGG - chr1_2:54657936-54657955 Msa0077690:intron 35.0%
CATACCAGTTGATTATAGCA+AGG + chr1_2:54657943-54657962 None:intergenic 35.0%
TGAATTATTGTGATAGGACC+CGG - chr1_2:54658085-54658104 Msa0077690:intron 35.0%
GATGAGAATGTGATCCAAAT+AGG - chr1_2:54658108-54658127 Msa0077690:intron 35.0%
GTGTGATGTTAGAAGAATGT+AGG - chr1_2:54658168-54658187 Msa0077690:intron 35.0%
GAAAATAATGTGACGTGGTA+GGG - chr1_2:54658200-54658219 Msa0077690:intron 35.0%
AAAATAATGTGACGTGGTAG+GGG - chr1_2:54658201-54658220 Msa0077690:intron 35.0%
! TGTGACCATCTTGTTATAAG+CGG - chr1_2:54658245-54658264 Msa0077690:intron 35.0%
TCAGAGAAAATACACTTCTC+TGG + chr1_2:54658322-54658341 None:intergenic 35.0%
!! AGAAGTGTATTTTCTCTGAG+AGG - chr1_2:54658323-54658342 Msa0077690:intron 35.0%
ACGAAAGTAGATTGCAGAAA+GGG + chr1_2:54658380-54658399 None:intergenic 35.0%
AACGAAAGTAGATTGCAGAA+AGG + chr1_2:54658381-54658400 None:intergenic 35.0%
ATACCATATATGTAGCCACT+GGG + chr1_2:54658493-54658512 None:intergenic 35.0%
TAAACTAGTGTTGCCAATAG+TGG - chr1_2:54658686-54658705 Msa0077690:intron 35.0%
TTCTCTGCAAGCAGATAATA+GGG + chr1_2:54658767-54658786 None:intergenic 35.0%
TTTCTCTGCAAGCAGATAAT+AGG + chr1_2:54658768-54658787 None:intergenic 35.0%
! TTTTCTGCTTACATATGGAG+AGG - chr1_2:54658800-54658819 Msa0077690:intron 35.0%
AACGCAACATGGTAAATGAA+AGG + chr1_2:54658861-54658880 None:intergenic 35.0%
TGTCAAATTACAACTTCCTC+TGG + chr1_2:54658887-54658906 None:intergenic 35.0%
! GATGTAGCATAAAGCATCTT+CGG + chr1_2:54658990-54659009 None:intergenic 35.0%
GATGCTTTATGCTACATCAA+AGG - chr1_2:54658992-54659011 Msa0077690:intron 35.0%
TTTATGCTACATCAAAGGAC+AGG - chr1_2:54658997-54659016 Msa0077690:intron 35.0%
AGCTAAAATTCCTATGCAGA+GGG + chr1_2:54659312-54659331 None:intergenic 35.0%
TGTTCCTTATTGCCATATGA+TGG - chr1_2:54659331-54659350 Msa0077690:five_prime_UTR 35.0%
AGAACCATCATATGGCAATA+AGG + chr1_2:54659338-54659357 None:intergenic 35.0%
GGATATTGAAACTCTGTCAA+TGG - chr1_2:54659385-54659404 Msa0077690:five_prime_UTR 35.0%
TATTGAAACTCTGTCAATGG+AGG - chr1_2:54659388-54659407 Msa0077690:five_prime_UTR 35.0%
TTGTAGAGAGAGAAAGTGAG+AGG + chr1_2:54655585-54655604 None:intergenic 40.0%
ATAGCTATGGCTTTCAGAGT+GGG - chr1_2:54655646-54655665 Msa0077690:three_prime_UTR 40.0%
! CCAAAATCAGCATGGTCTTA+AGG + chr1_2:54655724-54655743 None:intergenic 40.0%
!!! CCTTAAGACCATGCTGATTT+TGG - chr1_2:54655721-54655740 Msa0077690:three_prime_UTR 40.0%
!! GTCGGATTTTATTGGTTGCA+TGG - chr1_2:54655772-54655791 Msa0077690:three_prime_UTR 40.0%
CTGAGACCCAAAATTTGTAG+TGG + chr1_2:54655816-54655835 None:intergenic 40.0%
TCAGCATTGTCAGTTGAGAT+AGG + chr1_2:54655954-54655973 None:intergenic 40.0%
TCAAAGCAACTTCATGCATG+AGG + chr1_2:54656261-54656280 None:intergenic 40.0%
AAGTGAATTGCCCACTTTAG+AGG + chr1_2:54656467-54656486 None:intergenic 40.0%
GTAAGATATATCTCATGCCC+CGG - chr1_2:54656734-54656753 Msa0077690:intron 40.0%
TAAGATATATCTCATGCCCC+GGG - chr1_2:54656735-54656754 Msa0077690:intron 40.0%
GGAAACTGTAAATATTCCCG+GGG + chr1_2:54656754-54656773 None:intergenic 40.0%
GGGAAACTGTAAATATTCCC+GGG + chr1_2:54656755-54656774 None:intergenic 40.0%
GGGGAAACTGTAAATATTCC+CGG + chr1_2:54656756-54656775 None:intergenic 40.0%
!! TGTTTGGCAGAGCATTCTTT+TGG + chr1_2:54656863-54656882 None:intergenic 40.0%
!!! ATGCTGTTTTCCTCATTGCT+GGG - chr1_2:54656882-54656901 Msa0077690:intron 40.0%
TGAAATGAATCCCAGCAATG+AGG + chr1_2:54656895-54656914 None:intergenic 40.0%
TTGTAGGGAAATGCATCTTC+TGG - chr1_2:54657006-54657025 Msa0077690:intron 40.0%
TGTAGGGAAATGCATCTTCT+GGG - chr1_2:54657007-54657026 Msa0077690:intron 40.0%
CTCGTTCAGATTTCTGTCTT+TGG + chr1_2:54657444-54657463 None:intergenic 40.0%
CAAAGACAGAAATCTGAACG+AGG - chr1_2:54657442-54657461 Msa0077690:intron 40.0%
AGGCTATGGGTTTATGCAAA+CGG + chr1_2:54657893-54657912 None:intergenic 40.0%
! GACCCCTTTCTTTTAGTATC+TGG - chr1_2:54657977-54657996 Msa0077690:intron 40.0%
GAATTATTGTGATAGGACCC+GGG - chr1_2:54658086-54658105 Msa0077690:intron 40.0%
TTTGGATCACATTCTCATCC+CGG + chr1_2:54658107-54658126 None:intergenic 40.0%
AGTTGTCCAAATCCATTGTG+TGG - chr1_2:54658144-54658163 Msa0077690:intron 40.0%
GTTGTCCAAATCCATTGTGT+GGG - chr1_2:54658145-54658164 Msa0077690:intron 40.0%
TTGTCCAAATCCATTGTGTG+GGG - chr1_2:54658146-54658165 Msa0077690:intron 40.0%
TAGAAGAATGTAGGAAACGG+AGG - chr1_2:54658177-54658196 Msa0077690:intron 40.0%
AGAAGAATGTAGGAAACGGA+GGG - chr1_2:54658178-54658197 Msa0077690:intron 40.0%
GGAAAATAATGTGACGTGGT+AGG - chr1_2:54658199-54658218 Msa0077690:intron 40.0%
! GTGACCATCTTGTTATAAGC+GGG - chr1_2:54658246-54658265 Msa0077690:intron 40.0%
TATCCCAGTGGCTACATATA+TGG - chr1_2:54658487-54658506 Msa0077690:intron 40.0%
GATACCATATATGTAGCCAC+TGG + chr1_2:54658494-54658513 None:intergenic 40.0%
GCAAAGTGTGAAGATAGAGA+TGG + chr1_2:54658826-54658845 None:intergenic 40.0%
ATCAAAGGACAGGTTCAGAA+GGG - chr1_2:54659007-54659026 Msa0077690:intron 40.0%
AATCACCTGAGCAAACTGAA+AGG - chr1_2:54659111-54659130 Msa0077690:intron 40.0%
ATCACCTGAGCAAACTGAAA+GGG - chr1_2:54659112-54659131 Msa0077690:intron 40.0%
! ATAACCCTTTCAGTTTGCTC+AGG + chr1_2:54659119-54659138 None:intergenic 40.0%
ATATCAGTATCAGCCTTCAC+AGG + chr1_2:54659270-54659289 None:intergenic 40.0%
TGTGAAGGCTGATACTGATA+TGG - chr1_2:54659269-54659288 Msa0077690:intron 40.0%
GTGAAGGCTGATACTGATAT+GGG - chr1_2:54659270-54659289 Msa0077690:intron 40.0%
GCTAAAATTCCTATGCAGAG+GGG + chr1_2:54659311-54659330 None:intergenic 40.0%
CAGCTAAAATTCCTATGCAG+AGG + chr1_2:54659313-54659332 None:intergenic 40.0%
ATTGCCATATGATGGTTCTC+AGG - chr1_2:54659339-54659358 Msa0077690:five_prime_UTR 40.0%
AGAGGAGAGAGAGAATTGTG+TGG + chr1_2:54655567-54655586 None:intergenic 45.0%
CATAGCTATGGCTTTCAGAG+TGG - chr1_2:54655645-54655664 Msa0077690:three_prime_UTR 45.0%
AACCTGTGAAGCATAGACAC+TGG - chr1_2:54656334-54656353 Msa0077690:intron 45.0%
ATTGCTACTAGCGTCAGTGT+CGG + chr1_2:54656368-54656387 None:intergenic 45.0%
! ATTGCCCACTTTAGAGGATC+CGG + chr1_2:54656461-54656480 None:intergenic 45.0%
!!! CATGCTGTTTTCCTCATTGC+TGG - chr1_2:54656881-54656900 Msa0077690:intron 45.0%
!! GGAAATGCATCTTCTGGGAT+GGG - chr1_2:54657012-54657031 Msa0077690:intron 45.0%
GCACATTTCAGGAATTGCAG+AGG - chr1_2:54657055-54657074 Msa0077690:intron 45.0%
GAGGTTGTGGTCGAAAAAAC+TGG - chr1_2:54657126-54657145 Msa0077690:intron 45.0%
GTTGTGGTCGAAAAAACTGG+AGG - chr1_2:54657129-54657148 Msa0077690:intron 45.0%
TTGGATCACATTCTCATCCC+GGG + chr1_2:54658106-54658125 None:intergenic 45.0%
TAACATCACACCCCACACAA+TGG + chr1_2:54658159-54658178 None:intergenic 45.0%
GGAGGGAAAATAATGTGACG+TGG - chr1_2:54658195-54658214 Msa0077690:intron 45.0%
CCTACCCGCTTATAACAAGA+TGG + chr1_2:54658253-54658272 None:intergenic 45.0%
! CCATCTTGTTATAAGCGGGT+AGG - chr1_2:54658250-54658269 Msa0077690:intron 45.0%
! TCTTGTTATAAGCGGGTAGG+AGG - chr1_2:54658253-54658272 Msa0077690:intron 45.0%
AGATTGTCGATCTTGAGACC+AGG + chr1_2:54658600-54658619 None:intergenic 45.0%
ACCATGTTGCGTTAAACCAG+AGG - chr1_2:54658868-54658887 Msa0077690:intron 45.0%
! TCCTCTGGTTTAACGCAACA+TGG + chr1_2:54658872-54658891 None:intergenic 45.0%
CATCAAAGGACAGGTTCAGA+AGG - chr1_2:54659006-54659025 Msa0077690:intron 45.0%
GGGAATCCACTATGAGATTC+AGG - chr1_2:54659037-54659056 Msa0077690:intron 45.0%
CTGTTGCCTGAATCTCATAG+TGG + chr1_2:54659046-54659065 None:intergenic 45.0%
! CTTCGAGTTCCATCTCTGTT+GGG + chr1_2:54659070-54659089 None:intergenic 45.0%
! ACTTCGAGTTCCATCTCTGT+TGG + chr1_2:54659071-54659090 None:intergenic 45.0%
TCCACCTGAGAACCATCATA+TGG + chr1_2:54659346-54659365 None:intergenic 45.0%
!! ACTCTGTCAATGGAGGTGTT+TGG - chr1_2:54659395-54659414 Msa0077690:five_prime_UTR 45.0%
CAGCAGCAGCTTCATAGCTA+TGG - chr1_2:54655633-54655652 Msa0077690:three_prime_UTR 50.0%
ATGGCTTTCAGAGTGGGAGT+TGG - chr1_2:54655652-54655671 Msa0077690:three_prime_UTR 50.0%
CTTTCAGAGTGGGAGTTGGA+GGG - chr1_2:54655656-54655675 Msa0077690:three_prime_UTR 50.0%
TTTCAGAGTGGGAGTTGGAG+GGG - chr1_2:54655657-54655676 Msa0077690:three_prime_UTR 50.0%
GTGTGTTAGACCCTTTCGCT+CGG - chr1_2:54655978-54655997 Msa0077690:CDS 50.0%
ACGATCGTTTCCGAGCGAAA+GGG + chr1_2:54655991-54656010 None:intergenic 50.0%
GTCCAGTGTCTATGCTTCAC+AGG + chr1_2:54656339-54656358 None:intergenic 50.0%
!! GGATCCGGATCCTCTAAAGT+GGG - chr1_2:54656454-54656473 Msa0077690:intron 50.0%
! TGCATGCACATTTTGTGCCC+TGG - chr1_2:54656710-54656729 Msa0077690:intron 50.0%
! GGGAAATGCATCTTCTGGGA+TGG - chr1_2:54657011-54657030 Msa0077690:intron 50.0%
GCAGAGCGTAAGCACATTTC+AGG - chr1_2:54657044-54657063 Msa0077690:intron 50.0%
ATGCAGTCCCCAGTTCAACA+AGG + chr1_2:54657771-54657790 None:intergenic 50.0%
! CACACCCCACACAATGGATT+TGG + chr1_2:54658153-54658172 None:intergenic 50.0%
TAATGTGACGTGGTAGGGGA+TGG - chr1_2:54658205-54658224 Msa0077690:intron 50.0%
AATGTGACGTGGTAGGGGAT+GGG - chr1_2:54658206-54658225 Msa0077690:intron 50.0%
TGTTATAAGCGGGTAGGAGG+AGG - chr1_2:54658256-54658275 Msa0077690:intron 50.0%
GTTATAAGCGGGTAGGAGGA+GGG - chr1_2:54658257-54658276 Msa0077690:intron 50.0%
TGAGAGGAGCCTAGCTATGT+GGG - chr1_2:54658339-54658358 Msa0077690:intron 50.0%
CGCATCATACTGTATCCCAG+TGG - chr1_2:54658475-54658494 Msa0077690:intron 50.0%
GGACGAATACGAGCTACAGA+AGG + chr1_2:54658969-54658988 None:intergenic 50.0%
GCCATATGATGGTTCTCAGG+TGG - chr1_2:54659342-54659361 Msa0077690:five_prime_UTR 50.0%
!! GCGAATTTAGCTTGGGGGAC+TGG - chr1_2:54655518-54655537 Msa0077690:three_prime_UTR 55.0%
GCTTTCAGAGTGGGAGTTGG+AGG - chr1_2:54655655-54655674 Msa0077690:three_prime_UTR 55.0%
CACGATCGTTTCCGAGCGAA+AGG + chr1_2:54655992-54656011 None:intergenic 55.0%
! GGGATCCGGATCCTCTAAAG+TGG - chr1_2:54656453-54656472 Msa0077690:intron 55.0%
ATGTGACGTGGTAGGGGATG+GGG - chr1_2:54658207-54658226 Msa0077690:intron 55.0%
CTGAGAGGAGCCTAGCTATG+TGG - chr1_2:54658338-54658357 Msa0077690:intron 55.0%
GAGCCTAGCTATGTGGGTAG+AGG - chr1_2:54658345-54658364 Msa0077690:intron 55.0%
CCTCCTCTACCCACATAGCT+AGG + chr1_2:54658351-54658370 None:intergenic 55.0%
CCTAGCTATGTGGGTAGAGG+AGG - chr1_2:54658348-54658367 Msa0077690:intron 55.0%
AGGTTCAGAAGGGAGCTGCA+GGG - chr1_2:54659017-54659036 Msa0077690:intron 55.0%
GGCAACAGACCCAACAGAGA+TGG - chr1_2:54659058-54659077 Msa0077690:intron 55.0%
GATGGAACTCGAAGTCCTCC+AGG - chr1_2:54659076-54659095 Msa0077690:intron 55.0%
ATTAGTTCGCACGCTCCTGG+AGG + chr1_2:54659094-54659113 None:intergenic 55.0%
! GTGATTAGTTCGCACGCTCC+TGG + chr1_2:54659097-54659116 None:intergenic 55.0%
CCTATGCAGAGGGGTCTCTT+AGG + chr1_2:54659302-54659321 None:intergenic 55.0%
CCTAAGAGACCCCTCTGCAT+AGG - chr1_2:54659299-54659318 Msa0077690:exon 55.0%
GGGTAGGAGGAGGGAGAGAA+AGG - chr1_2:54658266-54658285 Msa0077690:intron 60.0%
CAGGTTCAGAAGGGAGCTGC+AGG - chr1_2:54659016-54659035 Msa0077690:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 54655487 54659473 54655487 ID=Msa0077690;Name=Msa0077690
chr1_2 mRNA 54655487 54659473 54655487 ID=Msa0077690-mRNA-1;Parent=Msa0077690;Name=Msa0077690-mRNA-1;_AED=0.02;_eAED=0.02;_QI=165|1|1|1|1|1|3|361|147
chr1_2 exon 54655487 54655998 54655487 ID=Msa0077690-mRNA-1:exon:16963;Parent=Msa0077690-mRNA-1
chr1_2 exon 54657683 54657948 54657683 ID=Msa0077690-mRNA-1:exon:16962;Parent=Msa0077690-mRNA-1
chr1_2 exon 54659282 54659473 54659282 ID=Msa0077690-mRNA-1:exon:16961;Parent=Msa0077690-mRNA-1
chr1_2 five_prime_UTR 54659309 54659473 54659309 ID=Msa0077690-mRNA-1:five_prime_utr;Parent=Msa0077690-mRNA-1
chr1_2 CDS 54659282 54659308 54659282 ID=Msa0077690-mRNA-1:cds;Parent=Msa0077690-mRNA-1
chr1_2 CDS 54657683 54657948 54657683 ID=Msa0077690-mRNA-1:cds;Parent=Msa0077690-mRNA-1
chr1_2 CDS 54655848 54655998 54655848 ID=Msa0077690-mRNA-1:cds;Parent=Msa0077690-mRNA-1
chr1_2 three_prime_UTR 54655487 54655847 54655487 ID=Msa0077690-mRNA-1:three_prime_utr;Parent=Msa0077690-mRNA-1
Gene Sequence

>Msa0077690

ATGGCTTTCAGAGTGGGAGTTGGAGGGGGAAATGCATCTTCTGGGATGGGTGTTGCTGAGCAGAGCGTAAGCACATTTCAGGAATTGCAGAGGAAGAAAGTATATCGCTATGTGATTTTCAAAATTGATGAGAAAAAGAAAGAGGTTGTGGTCGAAAAAACTGGAGGCCCTTCTGAGAGCTATGATGATTTCACTGCATCCTTGCCTGAAAATGATTGTAGATATGCAGTCTTTGACTTTGATTTCGTGACTGCTGAGAACTGCCAAAAGAGCAAAATCTTTTTTATCGCATGGTCTCCTTCTGTAGCTCGTATTCGTCCGAAGATGCTTTATGCTACATCAAAGGACAGGTTCAGAAGGGAGCTGCAGGGAATCCACTATGAGATTCAGGCAACAGACCCAACAGAGATGGAACTCGAAGTCCTCCAGGAGCGTGCGAACTAA
Protein sequence

>Msa0077690

MAFRVGVGGGNASSGMGVAEQSVSTFQELQRKKVYRYVIFKIDEKKKEVVVEKTGGPSESYDDFTASLPENDCRYAVFDFDFVTAENCQKSKIFFIAWSPSVARIRPKMLYATSKDRFRRELQGIHYEIQATDPTEMELEVLQERAN*