AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0125660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0125660 MtrunA17_Chr1g0187771 99.320 147 1 0 1 147 1 147 1.16e-108 305
Msa0125660 MtrunA17_Chr7g0261251 78.912 147 30 1 1 147 1 146 1.76e-86 249
Msa0125660 MtrunA17_Chr1g0182501 62.319 138 52 0 10 147 2 139 1.44e-66 198
Msa0125660 MtrunA17_Chr4g0037251 61.644 146 50 1 1 146 3 142 2.99e-66 197
Msa0125660 MtrunA17_Chr5g0397641 61.314 137 51 1 10 146 2 136 1.21e-63 191
Msa0125660 MtrunA17_Chr8g0386651 60.584 137 52 1 10 146 2 136 1.17e-61 186
Msa0125660 MtrunA17_Chr1g0203211 57.447 141 58 1 6 146 22 160 5.36e-60 182
Msa0125660 MtrunA17_Chr2g0291571 55.797 138 61 0 10 147 2 139 9.00e-59 179
Msa0125660 MtrunA17_Chr8g0380291 55.072 138 62 0 10 147 2 139 5.26e-56 172
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0125660 AT2G31200.1 76.190 147 34 1 1 147 1 146 6.69e-82 238
Msa0125660 AT5G59890.1 61.594 138 53 0 10 147 2 139 3.00e-65 195
Msa0125660 AT3G46010.2 57.931 145 61 0 3 147 6 150 8.65e-65 194
Msa0125660 AT4G00680.2 58.042 143 60 0 5 147 7 149 1.33e-64 194
Msa0125660 AT3G46010.1 60.145 138 55 0 10 147 2 139 2.43e-64 193
Msa0125660 AT4G00680.1 58.696 138 57 0 10 147 2 139 5.13e-64 192
Msa0125660 AT1G01750.1 58.696 138 57 0 10 147 2 139 1.95e-63 191
Msa0125660 AT1G01750.2 58.696 138 57 0 10 147 7 144 2.17e-63 191
Msa0125660 AT2G16700.1 58.503 147 55 1 1 147 3 143 3.05e-63 190
Msa0125660 AT4G25590.1 62.774 137 49 1 10 146 2 136 3.65e-63 190
Msa0125660 AT3G46000.1 60.870 138 52 1 10 147 2 137 2.68e-62 188
Msa0125660 AT3G46000.2 60.870 138 52 1 10 147 21 156 3.76e-62 188
Msa0125660 AT5G59890.3 61.364 132 51 0 16 147 1 132 2.15e-61 185
Msa0125660 AT5G59890.2 61.364 132 51 0 16 147 1 132 2.15e-61 185
Msa0125660 AT2G16700.4 61.364 132 51 0 16 147 1 132 1.13e-60 183
Msa0125660 AT2G16700.2 61.364 132 51 0 16 147 1 132 1.13e-60 183
Msa0125660 AT4G34970.1 58.394 137 57 0 11 147 5 141 2.00e-60 183
Msa0125660 AT5G52360.1 61.314 137 51 1 10 146 2 136 4.61e-60 182
Msa0125660 AT4G25590.2 62.595 131 47 1 16 146 1 129 1.72e-59 181
Msa0125660 AT5G59880.1 55.797 138 61 0 10 147 2 139 2.80e-59 180
Msa0125660 AT3G46000.3 60.606 132 50 1 16 147 1 130 1.34e-58 178
Msa0125660 AT5G59880.2 48.551 138 56 1 10 147 2 124 3.51e-46 146
Msa0125660 AT2G16700.3 50.427 117 51 2 1 117 3 112 9.61e-37 122
Msa0125660 AT3G45990.1 42.754 138 68 3 16 147 1 133 2.41e-34 117

Find 61 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCCTTAAGTACTATTATTA+TGG 0.209108 1_3:+54227133 Msa0125660:three_prime_UTR
TTGTAGGGAAATGCATCTTC+TGG 0.261866 1_3:+54224952 Msa0125660:intron
GCAGAGCGTAAGCACATTTC+AGG 0.286465 1_3:+54224990 Msa0125660:CDS
AACCATAATAATAGTACTTA+AGG 0.320026 1_3:-54227135 None:intergenic
TGTGAAGGCTGATACTGATA+TGG 0.329087 1_3:+54227215 Msa0125660:three_prime_UTR
AGAATTGTGTGGAGATGAAA+AGG 0.335598 1_3:-54223487 None:intergenic
AAGGGTTATGTCTATTAGTT+AGG 0.342272 1_3:+54227076 Msa0125660:three_prime_UTR
TGAAGAGTCAAGAATTTAGA+AGG 0.351710 1_3:-54223553 None:intergenic
GGGAATCCACTATGAGATTC+AGG 0.356002 1_3:+54226983 Msa0125660:CDS
CCTCTGCAGAGGGGTCTCTT+AGG 0.385406 1_3:-54227245 None:intergenic
AGAACCATCATATGGCAATA+AGG 0.388005 1_3:-54227281 None:intergenic
CAGGTTCAGAAGGGAGCTGC+AGG 0.400251 1_3:+54226962 Msa0125660:CDS
GCTTTCAGAGTGGGAGTTGG+AGG 0.419649 1_3:+54223601 Msa0125660:CDS
GTGATTAGTTCGCACGCTCC+TGG 0.421098 1_3:-54227040 None:intergenic
TGTTCCTTATTGCCATATGA+TGG 0.447267 1_3:+54227277 Msa0125660:three_prime_UTR
ACCATAATAATAGTACTTAA+GGG 0.452507 1_3:-54227134 None:intergenic
GATGTAGCATAAAGCATCTT+CGG 0.454388 1_3:-54226933 None:intergenic
ACTTCGAGTTCCATCTCTGT+TGG 0.461999 1_3:-54227014 None:intergenic
ATTGCCATATGATGGTTCTC+AGG 0.462653 1_3:+54227285 Msa0125660:three_prime_UTR
CTTCGAGTTCCATCTCTGTT+GGG 0.469550 1_3:-54227013 None:intergenic
ATAACCCTTTCAGTTTGCTC+AGG 0.490136 1_3:-54227062 None:intergenic
AAAGTGAGAGTGAGAGTGAG+AGG 0.500455 1_3:-54223516 None:intergenic
TGTAGGGAAATGCATCTTCT+GGG 0.504974 1_3:+54224953 Msa0125660:intron
GGATATTGAAACTCTGTCAA+TGG 0.511931 1_3:+54227331 Msa0125660:three_prime_UTR
CTTTCAGAGTGGGAGTTGGA+GGG 0.516133 1_3:+54223602 Msa0125660:CDS
ATCACCTGAGCAAACTGAAA+GGG 0.520857 1_3:+54227058 Msa0125660:exon
TAAATAACATTCACCTGTGA+AGG 0.522452 1_3:+54227200 Msa0125660:three_prime_UTR
TCCACCTGAGAACCATCATA+TGG 0.526072 1_3:-54227289 None:intergenic
ATGGCTTTCAGAGTGGGAGT+TGG 0.530306 1_3:+54223598 Msa0125660:CDS
TTTCAGAGTGGGAGTTGGAG+GGG 0.532929 1_3:+54223603 Msa0125660:CDS
ATAGCTATGGCTTTCAGAGT+GGG 0.535262 1_3:+54223592 Msa0125660:exon
CAGCAGCAGCTTCATAGCTA+TGG 0.536263 1_3:+54223579 Msa0125660:exon
GGGAAATGCATCTTCTGGGA+TGG 0.538512 1_3:+54224957 Msa0125660:intron
CATCAAAGGACAGGTTCAGA+AGG 0.548452 1_3:+54226952 Msa0125660:CDS
AGCTAAAATTCCTCTGCAGA+GGG 0.551878 1_3:-54227255 None:intergenic
GGAAATGCATCTTCTGGGAT+GGG 0.555903 1_3:+54224958 Msa0125660:CDS
GGCAACAGACCCAACAGAGA+TGG 0.557284 1_3:+54227004 Msa0125660:CDS
GATGGAACTCGAAGTCCTCC+AGG 0.560122 1_3:+54227022 Msa0125660:CDS
AAATCATCATAGCTCTCAGA+AGG 0.561770 1_3:-54225099 None:intergenic
GTGAAGGCTGATACTGATAT+GGG 0.562558 1_3:+54227216 Msa0125660:three_prime_UTR
GGACGAATACGAGCTACAGA+AGG 0.568172 1_3:-54226912 None:intergenic
CATAGCTATGGCTTTCAGAG+TGG 0.573358 1_3:+54223591 Msa0125660:exon
GAATTGTGTGGAGATGAAAA+GGG 0.573731 1_3:-54223486 None:intergenic
AGAGGAGAGAGAGAATTGTG+TGG 0.574529 1_3:-54223498 None:intergenic
CTGTTGCCTGAATCTCATAG+TGG 0.585469 1_3:-54226989 None:intergenic
TTTATGCTACATCAAAGGAC+AGG 0.592719 1_3:+54226943 Msa0125660:CDS
ATATCAGTATCAGCCTTCAC+AGG 0.593659 1_3:-54227213 None:intergenic
GATGCTTTATGCTACATCAA+AGG 0.601779 1_3:+54226938 Msa0125660:CDS
CAGCTAAAATTCCTCTGCAG+AGG 0.602388 1_3:-54227256 None:intergenic
ACTCTGTCAATGGAGGTGTT+TGG 0.602656 1_3:+54227341 Msa0125660:three_prime_UTR
AATCACCTGAGCAAACTGAA+AGG 0.609728 1_3:+54227057 Msa0125660:exon
TTTGTTGAAAACATTTCTTG+AGG 0.612163 1_3:-54227109 None:intergenic
ATCAAAGGACAGGTTCAGAA+GGG 0.621100 1_3:+54226953 Msa0125660:CDS
CCTAAGAGACCCCTCTGCAG+AGG 0.626974 1_3:+54227245 Msa0125660:three_prime_UTR
GCCATATGATGGTTCTCAGG+TGG 0.634844 1_3:+54227288 Msa0125660:three_prime_UTR
GCACATTTCAGGAATTGCAG+AGG 0.661602 1_3:+54225001 Msa0125660:CDS
ATTAGTTCGCACGCTCCTGG+AGG 0.663424 1_3:-54227037 None:intergenic
GCTAAAATTCCTCTGCAGAG+GGG 0.687024 1_3:-54227254 None:intergenic
AGGTTCAGAAGGGAGCTGCA+GGG 0.689968 1_3:+54226963 Msa0125660:CDS
TATTGAAACTCTGTCAATGG+AGG 0.714240 1_3:+54227334 Msa0125660:three_prime_UTR
AATCATCATAGCTCTCAGAA+GGG 0.724970 1_3:-54225098 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGGTTAAAAATTATTTT+AGG - chr1_3:54223430-54223449 None:intergenic 10.0%
!! AAACATGATTGAAATATATA+TGG - chr1_3:54223849-54223868 None:intergenic 15.0%
!!! ATTTGAATAAAATTTTCTGA+GGG + chr1_3:54224035-54224054 Msa0125660:intron 15.0%
!!! TAAAAAATGCTTTTGATATT+TGG + chr1_3:54225788-54225807 Msa0125660:intron 15.0%
!! AACACAAAAATGAATAAAAA+AGG - chr1_3:54226479-54226498 None:intergenic 15.0%
!!! TTAATTAGTTTATATACTGT+AGG + chr1_3:54226517-54226536 Msa0125660:intron 15.0%
!! AAAAAATGATAGAATGAAGA+TGG - chr1_3:54223636-54223655 None:intergenic 20.0%
!!! CATTTGAATAAAATTTTCTG+AGG + chr1_3:54224034-54224053 Msa0125660:intron 20.0%
!! ACTTAATAATCATATCAAAG+GGG - chr1_3:54224376-54224395 None:intergenic 20.0%
!! CACTTAATAATCATATCAAA+GGG - chr1_3:54224377-54224396 None:intergenic 20.0%
!! ACACTTAATAATCATATCAA+AGG - chr1_3:54224378-54224397 None:intergenic 20.0%
!! TTGATATGATTATTAAGTGT+TGG + chr1_3:54224378-54224397 Msa0125660:intron 20.0%
!! TGATATGATTATTAAGTGTT+GGG + chr1_3:54224379-54224398 Msa0125660:intron 20.0%
!!! GATTATTTTGGTTTGAAAAA+AGG + chr1_3:54224896-54224915 Msa0125660:intron 20.0%
!!! TAAAAAAGATTTTGCTCTTT+TGG - chr1_3:54225197-54225216 None:intergenic 20.0%
!! ACATATGTATATATTGCATT+TGG + chr1_3:54225312-54225331 Msa0125660:intron 20.0%
!!! AAATAAAAAACCCTTTTAGA+AGG - chr1_3:54225859-54225878 None:intergenic 20.0%
!! AGACAAATATAATCAAGTAT+TGG - chr1_3:54225966-54225985 None:intergenic 20.0%
!! ATACTTGATTATATTTGTCT+CGG + chr1_3:54225966-54225985 Msa0125660:intron 20.0%
!! AATAATTCATATGAATGGAA+AGG - chr1_3:54226020-54226039 None:intergenic 20.0%
!! ATCACAATAATTCATATGAA+TGG - chr1_3:54226025-54226044 None:intergenic 20.0%
!! TTCATATGAATTATTGTGAT+AGG + chr1_3:54226025-54226044 Msa0125660:intron 20.0%
!!! TTTTGTGTTGATTTTTCTTT+TGG + chr1_3:54226490-54226509 Msa0125660:intron 20.0%
!!! TTTGTGTTGATTTTTCTTTT+GGG + chr1_3:54226491-54226510 Msa0125660:intron 20.0%
!! ATTTATATACATCAGAATCA+TGG + chr1_3:54226573-54226592 Msa0125660:intron 20.0%
!! GACAATCTTAAAAAAAACTT+TGG + chr1_3:54226606-54226625 Msa0125660:intron 20.0%
!!! TTTTATTTTCTGCTTACATA+TGG + chr1_3:54226741-54226760 Msa0125660:intron 20.0%
!! ACCATAATAATAGTACTTAA+GGG - chr1_3:54227137-54227156 None:intergenic 20.0%
!! AACCATAATAATAGTACTTA+AGG - chr1_3:54227138-54227157 None:intergenic 20.0%
!!! GAAGTAATATGCTTATTTTA+TGG + chr1_3:54227310-54227329 Msa0125660:three_prime_UTR 20.0%
!!! TTGTTTTTGTCGGATTTTAT+TGG + chr1_3:54223712-54223731 Msa0125660:intron 25.0%
!!! TCAGTTTTAATTTTGCTTTG+TGG + chr1_3:54223782-54223801 Msa0125660:intron 25.0%
!! AAACATATATCTTCGCATTT+TGG - chr1_3:54224110-54224129 None:intergenic 25.0%
! TCCAATGATGTTAAGAAATT+AGG + chr1_3:54224139-54224158 Msa0125660:intron 25.0%
! CATAGAAAGAATGCAAAAAT+GGG - chr1_3:54224252-54224271 None:intergenic 25.0%
! AAGTTAAATGTAGATAGTGT+AGG + chr1_3:54224471-54224490 Msa0125660:intron 25.0%
! GTATAAAGTGAGAAAATCAA+GGG - chr1_3:54224530-54224549 None:intergenic 25.0%
! AGTATAAAGTGAGAAAATCA+AGG - chr1_3:54224531-54224550 None:intergenic 25.0%
! ACTAAATCACCAATCAAAAA+GGG - chr1_3:54224722-54224741 None:intergenic 25.0%
! AACTAAATCACCAATCAAAA+AGG - chr1_3:54224723-54224742 None:intergenic 25.0%
!!! AGGACTTTAGTATTCTTTTT+TGG + chr1_3:54224866-54224885 Msa0125660:intron 25.0%
!!! GGACTTTAGTATTCTTTTTT+GGG + chr1_3:54224867-54224886 Msa0125660:intron 25.0%
!!! GACTTTAGTATTCTTTTTTG+GGG + chr1_3:54224868-54224887 Msa0125660:intron 25.0%
!!! ACTTTAGTATTCTTTTTTGG+GGG + chr1_3:54224869-54224888 Msa0125660:intron 25.0%
!!! TTTTGGTTTGAAAAAAGGAT+TGG + chr1_3:54224901-54224920 Msa0125660:intron 25.0%
! AATTTCGTGTAACTAATATG+TGG + chr1_3:54224924-54224943 Msa0125660:intron 25.0%
!!! TAATATGTGGATTTGTTGTA+GGG + chr1_3:54224937-54224956 Msa0125660:intron 25.0%
! AATTGATGAGAAAAAGAAAG+AGG + chr1_3:54225053-54225072 Msa0125660:CDS 25.0%
!!! ATCTATCTTATTGACTTTCA+TGG + chr1_3:54225457-54225476 Msa0125660:intron 25.0%
! AGCATATTGCTAATTTACTA+GGG + chr1_3:54225590-54225609 Msa0125660:intron 25.0%
!!! CTGGAATTTGAAATGATTTT+TGG - chr1_3:54225760-54225779 None:intergenic 25.0%
! AAACAACAATCACATGAATT+TGG - chr1_3:54225995-54226014 None:intergenic 25.0%
! TGATAAACTAATTGCCTATT+TGG - chr1_3:54226071-54226090 None:intergenic 25.0%
!!! TCTGATGTATTATTTCTGTT+TGG + chr1_3:54226242-54226261 Msa0125660:intron 25.0%
! CAATTGCAGAATATTATTGA+TGG - chr1_3:54226351-54226370 None:intergenic 25.0%
! ATACAGAATTAACAGTGATT+TGG - chr1_3:54226386-54226405 None:intergenic 25.0%
! TAATTCTGTATGTTTGGTAT+CGG + chr1_3:54226395-54226414 Msa0125660:intron 25.0%
!!! ATTTTTTTGCAATCCACTAT+TGG - chr1_3:54226648-54226667 None:intergenic 25.0%
! ATTATAGTATTGTAGCGTAA+CGG - chr1_3:54226688-54226707 None:intergenic 25.0%
! AGTAAACTCAAGCAAAATAA+AGG - chr1_3:54226868-54226887 None:intergenic 25.0%
!! ATTTTGCTTGAGTTTACTAA+TGG + chr1_3:54226870-54226889 Msa0125660:intron 25.0%
! TTTGTTGAAAACATTTCTTG+AGG - chr1_3:54227112-54227131 None:intergenic 25.0%
!! CATTTTTGCGAATTTAGCTT+GGG + chr1_3:54223445-54223464 Msa0125660:five_prime_UTR 30.0%
!!! ATTTTTGCGAATTTAGCTTG+GGG + chr1_3:54223446-54223465 Msa0125660:five_prime_UTR 30.0%
TGAAGAGTCAAGAATTTAGA+AGG - chr1_3:54223556-54223575 None:intergenic 30.0%
AAATCAAACCAAAATCAGCA+TGG - chr1_3:54223680-54223699 None:intergenic 30.0%
! TTCTTCCCACTACAAATTTT+GGG + chr1_3:54223757-54223776 Msa0125660:intron 30.0%
AAGAAAAACCGCATAATATG+AGG - chr1_3:54223814-54223833 None:intergenic 30.0%
GCCTAATTTCTTAACATCAT+TGG - chr1_3:54224143-54224162 None:intergenic 30.0%
TTGTCAAATGAAATCTTGTG+CGG + chr1_3:54224169-54224188 Msa0125660:intron 30.0%
! TTGAGTTACAAGTAGCTTTA+AGG + chr1_3:54224223-54224242 Msa0125660:intron 30.0%
CCATAGAAAGAATGCAAAAA+TGG - chr1_3:54224253-54224272 None:intergenic 30.0%
!! CCATTTTTGCATTCTTTCTA+TGG + chr1_3:54224250-54224269 Msa0125660:intron 30.0%
! TTATACTCTAGTCACTTTAG+AGG + chr1_3:54224544-54224563 Msa0125660:intron 30.0%
AGAAGTTTATGACTACACTT+CGG - chr1_3:54224578-54224597 None:intergenic 30.0%
ATGAGATATATCTTACTCCA+GGG - chr1_3:54224676-54224695 None:intergenic 30.0%
CTAAATCACCAATCAAAAAG+GGG - chr1_3:54224721-54224740 None:intergenic 30.0%
!! CTAATATGTGGATTTGTTGT+AGG + chr1_3:54224936-54224955 Msa0125660:intron 30.0%
! GCATATCTACAATCATTTTC+AGG - chr1_3:54225138-54225157 None:intergenic 30.0%
!!! GCAAAATCTTTTTTATCGCA+TGG + chr1_3:54225202-54225221 Msa0125660:CDS 30.0%
! TTGCTCAATTTTTCTTGCAT+TGG + chr1_3:54225270-54225289 Msa0125660:intron 30.0%
ATGCCTATTCTTAATCTTCA+TGG + chr1_3:54225502-54225521 Msa0125660:intron 30.0%
TGCCTATTCTTAATCTTCAT+GGG + chr1_3:54225503-54225522 Msa0125660:intron 30.0%
TATTCTTAATCTTCATGGGT+GGG + chr1_3:54225507-54225526 Msa0125660:intron 30.0%
GAGCATATTGCTAATTTACT+AGG + chr1_3:54225589-54225608 Msa0125660:intron 30.0%
!!! AGCATTTTTTATCAAGAGTC+TGG - chr1_3:54225779-54225798 None:intergenic 30.0%
! TGCTTTTGATATTTGGCAAA+AGG + chr1_3:54225795-54225814 Msa0125660:intron 30.0%
AACCAGATACTAAAAGAAAG+GGG - chr1_3:54225928-54225947 None:intergenic 30.0%
CAACCAGATACTAAAAGAAA+GGG - chr1_3:54225929-54225948 None:intergenic 30.0%
ACAACCAGATACTAAAAGAA+AGG - chr1_3:54225930-54225949 None:intergenic 30.0%
TGTTAGAAGAATGTAGGAAA+CGG + chr1_3:54226120-54226139 Msa0125660:intron 30.0%
CACTGTTAATTCTGTATGTT+TGG + chr1_3:54226389-54226408 Msa0125660:intron 30.0%
!! AGTGGATTGCAAAAAAATAG+CGG + chr1_3:54226650-54226669 Msa0125660:intron 30.0%
! CTAATGGATAATTGCTTTAC+AGG + chr1_3:54226886-54226905 Msa0125660:intron 30.0%
AAGGGTTATGTCTATTAGTT+AGG + chr1_3:54227076-54227095 Msa0125660:three_prime_UTR 30.0%
GCCCTTAAGTACTATTATTA+TGG + chr1_3:54227133-54227152 Msa0125660:three_prime_UTR 30.0%
TAAATAACATTCACCTGTGA+AGG + chr1_3:54227200-54227219 Msa0125660:three_prime_UTR 30.0%
GAACTAGAAAAATACGAAAC+AGG - chr1_3:54227383-54227402 None:intergenic 30.0%
CCAAGCTAAATTCGCAAAAA+TGG - chr1_3:54223447-54223466 None:intergenic 35.0%
!! CCATTTTTGCGAATTTAGCT+TGG + chr1_3:54223444-54223463 Msa0125660:five_prime_UTR 35.0%
!!! TTTTTGCGAATTTAGCTTGG+GGG + chr1_3:54223447-54223466 Msa0125660:five_prime_UTR 35.0%
GAATTGTGTGGAGATGAAAA+GGG - chr1_3:54223489-54223508 None:intergenic 35.0%
AGAATTGTGTGGAGATGAAA+AGG - chr1_3:54223490-54223509 None:intergenic 35.0%
!!! TCGAATGTCGTTGTTTTTGT+CGG + chr1_3:54223702-54223721 Msa0125660:intron 35.0%
! GTTCTTCCCACTACAAATTT+TGG + chr1_3:54223756-54223775 Msa0125660:intron 35.0%
TGAGACCCAAAATTTGTAGT+GGG - chr1_3:54223765-54223784 None:intergenic 35.0%
! GGTTGATTCCTCATATTATG+CGG + chr1_3:54223803-54223822 Msa0125660:intron 35.0%
GCATGTCTTTAGTACTTTCT+TGG + chr1_3:54224057-54224076 Msa0125660:intron 35.0%
TCTTTAGTACTTTCTTGGAC+AGG + chr1_3:54224062-54224081 Msa0125660:intron 35.0%
TCAAAGCAACTTAAAGCATG+AGG - chr1_3:54224207-54224226 None:intergenic 35.0%
!! GATTATTAAGTGTTGGGATC+CGG + chr1_3:54224385-54224404 Msa0125660:intron 35.0%
TTATGACTACACTTCGGATT+TGG - chr1_3:54224572-54224591 None:intergenic 35.0%
CATGAGATATATCTTACTCC+AGG - chr1_3:54224677-54224696 None:intergenic 35.0%
! TACAGTTTCCCCTTTTTGAT+TGG + chr1_3:54224710-54224729 Msa0125660:intron 35.0%
AATGAGGAAAACAGCATGTT+TGG - chr1_3:54224825-54224844 None:intergenic 35.0%
CTGGGATTCATTTCATTGAA+AGG + chr1_3:54224846-54224865 Msa0125660:intron 35.0%
!! TTTGGGGGAGTTGATTATTT+TGG + chr1_3:54224884-54224903 Msa0125660:intron 35.0%
TGAGAAAAAGAAAGAGGTTG+TGG + chr1_3:54225059-54225078 Msa0125660:CDS 35.0%
AATCATCATAGCTCTCAGAA+GGG - chr1_3:54225101-54225120 None:intergenic 35.0%
AAATCATCATAGCTCTCAGA+AGG - chr1_3:54225102-54225121 None:intergenic 35.0%
!! TCTACAATCATTTTCAGGCA+AGG - chr1_3:54225133-54225152 None:intergenic 35.0%
AAAGACAGAAATCTGAACGA+GGG + chr1_3:54225389-54225408 Msa0125660:intron 35.0%
CACCCATGAAGATTAAGAAT+AGG - chr1_3:54225508-54225527 None:intergenic 35.0%
CTATTCTTAATCTTCATGGG+TGG + chr1_3:54225506-54225525 Msa0125660:intron 35.0%
! CTGTATTTTCGAATGGAGTA+GGG + chr1_3:54225624-54225643 Msa0125660:intron 35.0%
! TGTATTTTCGAATGGAGTAG+GGG + chr1_3:54225625-54225644 Msa0125660:intron 35.0%
ACTATAGTACCTTGTTGAAC+TGG + chr1_3:54225705-54225724 Msa0125660:intron 35.0%
CTATAGTACCTTGTTGAACT+GGG + chr1_3:54225706-54225725 Msa0125660:intron 35.0%
TATAGTACCTTGTTGAACTG+GGG + chr1_3:54225707-54225726 Msa0125660:intron 35.0%
GTTTATGCAAACGGTTCAAA+CGG - chr1_3:54225830-54225849 None:intergenic 35.0%
TAAACCCATAGCCTTCTAAA+AGG + chr1_3:54225845-54225864 Msa0125660:intron 35.0%
! AAACCCATAGCCTTCTAAAA+GGG + chr1_3:54225846-54225865 Msa0125660:intron 35.0%
!! AAACCCTTTTAGAAGGCTAT+GGG - chr1_3:54225852-54225871 None:intergenic 35.0%
!! AAAACCCTTTTAGAAGGCTA+TGG - chr1_3:54225853-54225872 None:intergenic 35.0%
! ACAACCTTGCTATAATCAAC+TGG + chr1_3:54225882-54225901 Msa0125660:intron 35.0%
CATACCAGTTGATTATAGCA+AGG - chr1_3:54225889-54225908 None:intergenic 35.0%
TGAATTATTGTGATAGGACC+CGG + chr1_3:54226031-54226050 Msa0125660:intron 35.0%
GATGAGAATGTGATCCAAAT+AGG + chr1_3:54226054-54226073 Msa0125660:intron 35.0%
GTGTGATGTTAGAAGAATGT+AGG + chr1_3:54226114-54226133 Msa0125660:intron 35.0%
GAAAATAATGTGACGTGGTA+GGG + chr1_3:54226146-54226165 Msa0125660:intron 35.0%
AAAATAATGTGACGTGGTAG+GGG + chr1_3:54226147-54226166 Msa0125660:intron 35.0%
! TGTGACCATCTTGTTATAAG+CGG + chr1_3:54226191-54226210 Msa0125660:intron 35.0%
TCAGAGAAAATACACTTCTC+TGG - chr1_3:54226268-54226287 None:intergenic 35.0%
!! AGAAGTGTATTTTCTCTGAG+AGG + chr1_3:54226269-54226288 Msa0125660:intron 35.0%
ACGAAAGTAGATTGCAGAAA+GGG - chr1_3:54226326-54226345 None:intergenic 35.0%
AACGAAAGTAGATTGCAGAA+AGG - chr1_3:54226327-54226346 None:intergenic 35.0%
ATACCATATATGTAGCCACT+GGG - chr1_3:54226439-54226458 None:intergenic 35.0%
TTTATATACTGTAGGCATCC+TGG + chr1_3:54226525-54226544 Msa0125660:intron 35.0%
TAAACTAGTGTTGCCAATAG+TGG + chr1_3:54226632-54226651 Msa0125660:intron 35.0%
TTCTCTGCAAGCAGATAATA+GGG - chr1_3:54226713-54226732 None:intergenic 35.0%
TTTCTCTGCAAGCAGATAAT+AGG - chr1_3:54226714-54226733 None:intergenic 35.0%
! TTTTCTGCTTACATATGGAG+AGG + chr1_3:54226746-54226765 Msa0125660:intron 35.0%
AACGCAACATGGTAAATGAA+AGG - chr1_3:54226807-54226826 None:intergenic 35.0%
TGTCAAATTACAACTTCCTC+TGG - chr1_3:54226833-54226852 None:intergenic 35.0%
! GATGTAGCATAAAGCATCTT+CGG - chr1_3:54226936-54226955 None:intergenic 35.0%
GATGCTTTATGCTACATCAA+AGG + chr1_3:54226938-54226957 Msa0125660:CDS 35.0%
TTTATGCTACATCAAAGGAC+AGG + chr1_3:54226943-54226962 Msa0125660:CDS 35.0%
TGTTCCTTATTGCCATATGA+TGG + chr1_3:54227277-54227296 Msa0125660:three_prime_UTR 35.0%
AGAACCATCATATGGCAATA+AGG - chr1_3:54227284-54227303 None:intergenic 35.0%
GGATATTGAAACTCTGTCAA+TGG + chr1_3:54227331-54227350 Msa0125660:three_prime_UTR 35.0%
TATTGAAACTCTGTCAATGG+AGG + chr1_3:54227334-54227353 Msa0125660:three_prime_UTR 35.0%
ATAGCTATGGCTTTCAGAGT+GGG + chr1_3:54223592-54223611 Msa0125660:exon 40.0%
! CCAAAATCAGCATGGTCTTA+AGG - chr1_3:54223672-54223691 None:intergenic 40.0%
!!! CCTTAAGACCATGCTGATTT+TGG + chr1_3:54223669-54223688 Msa0125660:intron 40.0%
!! GTCGGATTTTATTGGTTGCA+TGG + chr1_3:54223720-54223739 Msa0125660:intron 40.0%
CTGAGACCCAAAATTTGTAG+TGG - chr1_3:54223766-54223785 None:intergenic 40.0%
TCAGCATTGTCAGTTGAGAT+AGG - chr1_3:54223904-54223923 None:intergenic 40.0%
AAGTGAATTGCCCACTTTAG+AGG - chr1_3:54224413-54224432 None:intergenic 40.0%
GTAAGATATATCTCATGCCC+CGG + chr1_3:54224680-54224699 Msa0125660:intron 40.0%
TAAGATATATCTCATGCCCC+GGG + chr1_3:54224681-54224700 Msa0125660:intron 40.0%
GGAAACTGTAAATATTCCCG+GGG - chr1_3:54224700-54224719 None:intergenic 40.0%
GGGAAACTGTAAATATTCCC+GGG - chr1_3:54224701-54224720 None:intergenic 40.0%
GGGGAAACTGTAAATATTCC+CGG - chr1_3:54224702-54224721 None:intergenic 40.0%
!! TGTTTGGCAGAGCATTCTTT+TGG - chr1_3:54224809-54224828 None:intergenic 40.0%
!!! ATGCTGTTTTCCTCATTGCT+GGG + chr1_3:54224828-54224847 Msa0125660:intron 40.0%
TGAAATGAATCCCAGCAATG+AGG - chr1_3:54224841-54224860 None:intergenic 40.0%
TTGTAGGGAAATGCATCTTC+TGG + chr1_3:54224952-54224971 Msa0125660:intron 40.0%
TGTAGGGAAATGCATCTTCT+GGG + chr1_3:54224953-54224972 Msa0125660:intron 40.0%
CTCGTTCAGATTTCTGTCTT+TGG - chr1_3:54225390-54225409 None:intergenic 40.0%
CAAAGACAGAAATCTGAACG+AGG + chr1_3:54225388-54225407 Msa0125660:intron 40.0%
! TGCCAAGCTGTATTTTCGAA+TGG + chr1_3:54225617-54225636 Msa0125660:intron 40.0%
CTCCATTCGAAAATACAGCT+TGG - chr1_3:54225622-54225641 None:intergenic 40.0%
! GCTGTATTTTCGAATGGAGT+AGG + chr1_3:54225623-54225642 Msa0125660:intron 40.0%
AGGCTATGGGTTTATGCAAA+CGG - chr1_3:54225839-54225858 None:intergenic 40.0%
! GACCCCTTTCTTTTAGTATC+TGG + chr1_3:54225923-54225942 Msa0125660:intron 40.0%
GAATTATTGTGATAGGACCC+GGG + chr1_3:54226032-54226051 Msa0125660:intron 40.0%
TTTGGATCACATTCTCATCC+CGG - chr1_3:54226053-54226072 None:intergenic 40.0%
AGTTGTCCAAATCCATTGTG+TGG + chr1_3:54226090-54226109 Msa0125660:intron 40.0%
GTTGTCCAAATCCATTGTGT+GGG + chr1_3:54226091-54226110 Msa0125660:intron 40.0%
TTGTCCAAATCCATTGTGTG+GGG + chr1_3:54226092-54226111 Msa0125660:intron 40.0%
TAGAAGAATGTAGGAAACGG+AGG + chr1_3:54226123-54226142 Msa0125660:intron 40.0%
AGAAGAATGTAGGAAACGGA+GGG + chr1_3:54226124-54226143 Msa0125660:intron 40.0%
GGAAAATAATGTGACGTGGT+AGG + chr1_3:54226145-54226164 Msa0125660:intron 40.0%
! GTGACCATCTTGTTATAAGC+GGG + chr1_3:54226192-54226211 Msa0125660:intron 40.0%
TATCCCAGTGGCTACATATA+TGG + chr1_3:54226433-54226452 Msa0125660:intron 40.0%
GATACCATATATGTAGCCAC+TGG - chr1_3:54226440-54226459 None:intergenic 40.0%
GCAAAGTGTGAAGATAGAGA+TGG - chr1_3:54226772-54226791 None:intergenic 40.0%
ATCAAAGGACAGGTTCAGAA+GGG + chr1_3:54226953-54226972 Msa0125660:CDS 40.0%
AATCACCTGAGCAAACTGAA+AGG + chr1_3:54227057-54227076 Msa0125660:exon 40.0%
ATCACCTGAGCAAACTGAAA+GGG + chr1_3:54227058-54227077 Msa0125660:exon 40.0%
! ATAACCCTTTCAGTTTGCTC+AGG - chr1_3:54227065-54227084 None:intergenic 40.0%
ATATCAGTATCAGCCTTCAC+AGG - chr1_3:54227216-54227235 None:intergenic 40.0%
TGTGAAGGCTGATACTGATA+TGG + chr1_3:54227215-54227234 Msa0125660:three_prime_UTR 40.0%
GTGAAGGCTGATACTGATAT+GGG + chr1_3:54227216-54227235 Msa0125660:three_prime_UTR 40.0%
AGCTAAAATTCCTCTGCAGA+GGG - chr1_3:54227258-54227277 None:intergenic 40.0%
ATTGCCATATGATGGTTCTC+AGG + chr1_3:54227285-54227304 Msa0125660:three_prime_UTR 40.0%
AGAGGAGAGAGAGAATTGTG+TGG - chr1_3:54223501-54223520 None:intergenic 45.0%
AAAGTGAGAGTGAGAGTGAG+AGG - chr1_3:54223519-54223538 None:intergenic 45.0%
CATAGCTATGGCTTTCAGAG+TGG + chr1_3:54223591-54223610 Msa0125660:exon 45.0%
AACCTGTGAAGCATAGACAC+TGG + chr1_3:54224280-54224299 Msa0125660:intron 45.0%
ATTGCTACTAGCGTCAGTGT+CGG - chr1_3:54224314-54224333 None:intergenic 45.0%
! ATTGCCCACTTTAGAGGATC+CGG - chr1_3:54224407-54224426 None:intergenic 45.0%
!!! CATGCTGTTTTCCTCATTGC+TGG + chr1_3:54224827-54224846 Msa0125660:intron 45.0%
!! GGAAATGCATCTTCTGGGAT+GGG + chr1_3:54224958-54224977 Msa0125660:CDS 45.0%
GCACATTTCAGGAATTGCAG+AGG + chr1_3:54225001-54225020 Msa0125660:CDS 45.0%
GAGGTTGTGGTCGAAAAAAC+TGG + chr1_3:54225072-54225091 Msa0125660:CDS 45.0%
GTTGTGGTCGAAAAAACTGG+AGG + chr1_3:54225075-54225094 Msa0125660:CDS 45.0%
TTGGATCACATTCTCATCCC+GGG - chr1_3:54226052-54226071 None:intergenic 45.0%
TAACATCACACCCCACACAA+TGG - chr1_3:54226105-54226124 None:intergenic 45.0%
GGAGGGAAAATAATGTGACG+TGG + chr1_3:54226141-54226160 Msa0125660:intron 45.0%
CCTACCCGCTTATAACAAGA+TGG - chr1_3:54226199-54226218 None:intergenic 45.0%
! CCATCTTGTTATAAGCGGGT+AGG + chr1_3:54226196-54226215 Msa0125660:intron 45.0%
! TCTTGTTATAAGCGGGTAGG+AGG + chr1_3:54226199-54226218 Msa0125660:intron 45.0%
AGATTGTCGATCTTGAGACC+AGG - chr1_3:54226546-54226565 None:intergenic 45.0%
ACCATGTTGCGTTAAACCAG+AGG + chr1_3:54226814-54226833 Msa0125660:intron 45.0%
! TCCTCTGGTTTAACGCAACA+TGG - chr1_3:54226818-54226837 None:intergenic 45.0%
CATCAAAGGACAGGTTCAGA+AGG + chr1_3:54226952-54226971 Msa0125660:CDS 45.0%
GGGAATCCACTATGAGATTC+AGG + chr1_3:54226983-54227002 Msa0125660:CDS 45.0%
CTGTTGCCTGAATCTCATAG+TGG - chr1_3:54226992-54227011 None:intergenic 45.0%
! CTTCGAGTTCCATCTCTGTT+GGG - chr1_3:54227016-54227035 None:intergenic 45.0%
! ACTTCGAGTTCCATCTCTGT+TGG - chr1_3:54227017-54227036 None:intergenic 45.0%
GCTAAAATTCCTCTGCAGAG+GGG - chr1_3:54227257-54227276 None:intergenic 45.0%
CAGCTAAAATTCCTCTGCAG+AGG - chr1_3:54227259-54227278 None:intergenic 45.0%
TCCACCTGAGAACCATCATA+TGG - chr1_3:54227292-54227311 None:intergenic 45.0%
!! ACTCTGTCAATGGAGGTGTT+TGG + chr1_3:54227341-54227360 Msa0125660:three_prime_UTR 45.0%
CAGCAGCAGCTTCATAGCTA+TGG + chr1_3:54223579-54223598 Msa0125660:exon 50.0%
ATGGCTTTCAGAGTGGGAGT+TGG + chr1_3:54223598-54223617 Msa0125660:CDS 50.0%
CTTTCAGAGTGGGAGTTGGA+GGG + chr1_3:54223602-54223621 Msa0125660:CDS 50.0%
TTTCAGAGTGGGAGTTGGAG+GGG + chr1_3:54223603-54223622 Msa0125660:CDS 50.0%
GTGTGTTAGACCCTTTCGCT+CGG + chr1_3:54223928-54223947 Msa0125660:intron 50.0%
ACGATCGTTTCCGAGCGAAA+GGG - chr1_3:54223941-54223960 None:intergenic 50.0%
GTCCAGTGTCTATGCTTCAC+AGG - chr1_3:54224285-54224304 None:intergenic 50.0%
!! GGATCCGGATCCTCTAAAGT+GGG + chr1_3:54224400-54224419 Msa0125660:intron 50.0%
! TGCATGCACATTTTGTGCCC+TGG + chr1_3:54224656-54224675 Msa0125660:intron 50.0%
! GGGAAATGCATCTTCTGGGA+TGG + chr1_3:54224957-54224976 Msa0125660:intron 50.0%
GCAGAGCGTAAGCACATTTC+AGG + chr1_3:54224990-54225009 Msa0125660:CDS 50.0%
ATGCAGTCCCCAGTTCAACA+AGG - chr1_3:54225717-54225736 None:intergenic 50.0%
! CACACCCCACACAATGGATT+TGG - chr1_3:54226099-54226118 None:intergenic 50.0%
TAATGTGACGTGGTAGGGGA+TGG + chr1_3:54226151-54226170 Msa0125660:intron 50.0%
AATGTGACGTGGTAGGGGAT+GGG + chr1_3:54226152-54226171 Msa0125660:intron 50.0%
TGTTATAAGCGGGTAGGAGG+AGG + chr1_3:54226202-54226221 Msa0125660:intron 50.0%
GTTATAAGCGGGTAGGAGGA+GGG + chr1_3:54226203-54226222 Msa0125660:intron 50.0%
TGAGAGGAGCCTAGCTATGT+GGG + chr1_3:54226285-54226304 Msa0125660:intron 50.0%
CGCATCATACTGTATCCCAG+TGG + chr1_3:54226421-54226440 Msa0125660:intron 50.0%
GGACGAATACGAGCTACAGA+AGG - chr1_3:54226915-54226934 None:intergenic 50.0%
GCCATATGATGGTTCTCAGG+TGG + chr1_3:54227288-54227307 Msa0125660:three_prime_UTR 50.0%
!! GCGAATTTAGCTTGGGGGAC+TGG + chr1_3:54223452-54223471 Msa0125660:five_prime_UTR 55.0%
GCTTTCAGAGTGGGAGTTGG+AGG + chr1_3:54223601-54223620 Msa0125660:CDS 55.0%
CACGATCGTTTCCGAGCGAA+AGG - chr1_3:54223942-54223961 None:intergenic 55.0%
! GGGATCCGGATCCTCTAAAG+TGG + chr1_3:54224399-54224418 Msa0125660:intron 55.0%
ATGTGACGTGGTAGGGGATG+GGG + chr1_3:54226153-54226172 Msa0125660:intron 55.0%
CTGAGAGGAGCCTAGCTATG+TGG + chr1_3:54226284-54226303 Msa0125660:intron 55.0%
GAGCCTAGCTATGTGGGTAG+AGG + chr1_3:54226291-54226310 Msa0125660:intron 55.0%
CCTCCTCTACCCACATAGCT+AGG - chr1_3:54226297-54226316 None:intergenic 55.0%
CCTAGCTATGTGGGTAGAGG+AGG + chr1_3:54226294-54226313 Msa0125660:intron 55.0%
AGGTTCAGAAGGGAGCTGCA+GGG + chr1_3:54226963-54226982 Msa0125660:CDS 55.0%
GGCAACAGACCCAACAGAGA+TGG + chr1_3:54227004-54227023 Msa0125660:CDS 55.0%
GATGGAACTCGAAGTCCTCC+AGG + chr1_3:54227022-54227041 Msa0125660:CDS 55.0%
ATTAGTTCGCACGCTCCTGG+AGG - chr1_3:54227040-54227059 None:intergenic 55.0%
! GTGATTAGTTCGCACGCTCC+TGG - chr1_3:54227043-54227062 None:intergenic 55.0%
GGGTAGGAGGAGGGAGAGAA+AGG + chr1_3:54226212-54226231 Msa0125660:intron 60.0%
CAGGTTCAGAAGGGAGCTGC+AGG + chr1_3:54226962-54226981 Msa0125660:CDS 60.0%
CCTCTGCAGAGGGGTCTCTT+AGG - chr1_3:54227248-54227267 None:intergenic 60.0%
CCTAAGAGACCCCTCTGCAG+AGG + chr1_3:54227245-54227264 Msa0125660:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 54223421 54227419 54223421 ID=Msa0125660;Name=Msa0125660
chr1_3 mRNA 54223421 54227419 54223421 ID=Msa0125660-mRNA-1;Parent=Msa0125660;Name=Msa0125660-mRNA-1;_AED=0.02;_eAED=0.02;_QI=177|1|1|1|1|1|3|361|147
chr1_3 exon 54223421 54223624 54223421 ID=Msa0125660-mRNA-1:exon:16689;Parent=Msa0125660-mRNA-1
chr1_3 exon 54224958 54225223 54224958 ID=Msa0125660-mRNA-1:exon:16690;Parent=Msa0125660-mRNA-1
chr1_3 exon 54226908 54227419 54226908 ID=Msa0125660-mRNA-1:exon:16691;Parent=Msa0125660-mRNA-1
chr1_3 five_prime_UTR 54223421 54223597 54223421 ID=Msa0125660-mRNA-1:five_prime_utr;Parent=Msa0125660-mRNA-1
chr1_3 CDS 54223598 54223624 54223598 ID=Msa0125660-mRNA-1:cds;Parent=Msa0125660-mRNA-1
chr1_3 CDS 54224958 54225223 54224958 ID=Msa0125660-mRNA-1:cds;Parent=Msa0125660-mRNA-1
chr1_3 CDS 54226908 54227058 54226908 ID=Msa0125660-mRNA-1:cds;Parent=Msa0125660-mRNA-1
chr1_3 three_prime_UTR 54227059 54227419 54227059 ID=Msa0125660-mRNA-1:three_prime_utr;Parent=Msa0125660-mRNA-1
Gene Sequence

>Msa0125660

ATGGCTTTCAGAGTGGGAGTTGGAGGGGGAAATGCATCTTCTGGGATGGGTGTTGCTGAGCAGAGCGTAAGCACATTTCAGGAATTGCAGAGGAAGAAAGTATATCGCTATGTGATTTTCAAAATTGATGAGAAAAAGAAAGAGGTTGTGGTCGAAAAAACTGGAGGCCCTTCTGAGAGCTATGATGATTTCACTGCATCCTTGCCTGAAAATGATTGTAGATATGCAGTCTTTGACTTTGATTTCGTGACTGCTGAGAACTGCCAAAAGAGCAAAATCTTTTTTATCGCATGGTCTCCTTCTGTAGCTCGTATTCGTCCGAAGATGCTTTATGCTACATCAAAGGACAGGTTCAGAAGGGAGCTGCAGGGAATCCACTATGAGATTCAGGCAACAGACCCAACAGAGATGGAACTCGAAGTCCTCCAGGAGCGTGCGAACTAA
Protein sequence

>Msa0125660

MAFRVGVGGGNASSGMGVAEQSVSTFQELQRKKVYRYVIFKIDEKKKEVVVEKTGGPSESYDDFTASLPENDCRYAVFDFDFVTAENCQKSKIFFIAWSPSVARIRPKMLYATSKDRFRRELQGIHYEIQATDPTEMELEVLQERAN*