AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0030170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0030170 MtrunA17_Chr1g0187771 99.320 147 1 0 1 147 1 147 1.16e-108 305
Msa0030170 MtrunA17_Chr7g0261251 78.912 147 30 1 1 147 1 146 1.76e-86 249
Msa0030170 MtrunA17_Chr1g0182501 62.319 138 52 0 10 147 2 139 1.44e-66 198
Msa0030170 MtrunA17_Chr4g0037251 61.644 146 50 1 1 146 3 142 2.99e-66 197
Msa0030170 MtrunA17_Chr5g0397641 61.314 137 51 1 10 146 2 136 1.21e-63 191
Msa0030170 MtrunA17_Chr8g0386651 60.584 137 52 1 10 146 2 136 1.17e-61 186
Msa0030170 MtrunA17_Chr1g0203211 57.447 141 58 1 6 146 22 160 5.36e-60 182
Msa0030170 MtrunA17_Chr2g0291571 55.797 138 61 0 10 147 2 139 9.00e-59 179
Msa0030170 MtrunA17_Chr8g0380291 55.072 138 62 0 10 147 2 139 5.26e-56 172
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0030170 AT2G31200.1 76.190 147 34 1 1 147 1 146 6.69e-82 238
Msa0030170 AT5G59890.1 61.594 138 53 0 10 147 2 139 3.00e-65 195
Msa0030170 AT3G46010.2 57.931 145 61 0 3 147 6 150 8.65e-65 194
Msa0030170 AT4G00680.2 58.042 143 60 0 5 147 7 149 1.33e-64 194
Msa0030170 AT3G46010.1 60.145 138 55 0 10 147 2 139 2.43e-64 193
Msa0030170 AT4G00680.1 58.696 138 57 0 10 147 2 139 5.13e-64 192
Msa0030170 AT1G01750.1 58.696 138 57 0 10 147 2 139 1.95e-63 191
Msa0030170 AT1G01750.2 58.696 138 57 0 10 147 7 144 2.17e-63 191
Msa0030170 AT2G16700.1 58.503 147 55 1 1 147 3 143 3.05e-63 190
Msa0030170 AT4G25590.1 62.774 137 49 1 10 146 2 136 3.65e-63 190
Msa0030170 AT3G46000.1 60.870 138 52 1 10 147 2 137 2.68e-62 188
Msa0030170 AT3G46000.2 60.870 138 52 1 10 147 21 156 3.76e-62 188
Msa0030170 AT5G59890.3 61.364 132 51 0 16 147 1 132 2.15e-61 185
Msa0030170 AT5G59890.2 61.364 132 51 0 16 147 1 132 2.15e-61 185
Msa0030170 AT2G16700.4 61.364 132 51 0 16 147 1 132 1.13e-60 183
Msa0030170 AT2G16700.2 61.364 132 51 0 16 147 1 132 1.13e-60 183
Msa0030170 AT4G34970.1 58.394 137 57 0 11 147 5 141 2.00e-60 183
Msa0030170 AT5G52360.1 61.314 137 51 1 10 146 2 136 4.61e-60 182
Msa0030170 AT4G25590.2 62.595 131 47 1 16 146 1 129 1.72e-59 181
Msa0030170 AT5G59880.1 55.797 138 61 0 10 147 2 139 2.80e-59 180
Msa0030170 AT3G46000.3 60.606 132 50 1 16 147 1 130 1.34e-58 178
Msa0030170 AT5G59880.2 48.551 138 56 1 10 147 2 124 3.51e-46 146
Msa0030170 AT2G16700.3 50.427 117 51 2 1 117 3 112 9.61e-37 122
Msa0030170 AT3G45990.1 42.754 138 68 3 16 147 1 133 2.41e-34 117

Find 60 sgRNAs with CRISPR-Local

Find 273 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCCTTAAGTACTATTATTA+TGG 0.209108 1_1:+56242050 Msa0030170:three_prime_UTR
TTGTAGGGAAATGCATCTTC+TGG 0.261866 1_1:+56239868 Msa0030170:intron
GCAGAGCGTAAGCACATTTC+AGG 0.286465 1_1:+56239906 Msa0030170:CDS
AACCATAATAATAGTACTTA+AGG 0.320026 1_1:-56242052 None:intergenic
TGTGAAGGCTGATACTGATA+TGG 0.329087 1_1:+56242132 Msa0030170:three_prime_UTR
AGAATTGTGTGGAGATGAAA+AGG 0.335598 1_1:-56238409 None:intergenic
AAGGGTTATGTCTATTAGTT+AGG 0.342272 1_1:+56241993 Msa0030170:three_prime_UTR
TGAAGAGTCAAGAATTTAGA+AGG 0.351710 1_1:-56238477 None:intergenic
GGGAATCCACTATGAGATTC+AGG 0.356002 1_1:+56241900 Msa0030170:CDS
CCTCTGCAGAGGGGTCTCTT+AGG 0.385406 1_1:-56242162 None:intergenic
AGAACCATCATATGGCAATA+AGG 0.388005 1_1:-56242198 None:intergenic
CAGGTTCAGAAGGGAGCTGC+AGG 0.400251 1_1:+56241879 Msa0030170:CDS
GCTTTCAGAGTGGGAGTTGG+AGG 0.419649 1_1:+56238525 Msa0030170:CDS
GTGATTAGTTCGCACGCTCC+TGG 0.421098 1_1:-56241957 None:intergenic
TGTTCCTTATTGCCATATGA+TGG 0.447267 1_1:+56242194 Msa0030170:three_prime_UTR
ACCATAATAATAGTACTTAA+GGG 0.452507 1_1:-56242051 None:intergenic
GATGTAGCATAAAGCATCTT+CGG 0.454388 1_1:-56241850 None:intergenic
ACTTCGAGTTCCATCTCTGT+TGG 0.461999 1_1:-56241931 None:intergenic
ATTGCCATATGATGGTTCTC+AGG 0.462653 1_1:+56242202 Msa0030170:three_prime_UTR
CTTCGAGTTCCATCTCTGTT+GGG 0.469550 1_1:-56241930 None:intergenic
ATAACCCTTTCAGTTTGCTC+AGG 0.490136 1_1:-56241979 None:intergenic
AAAGTGAGAGTGAGAGTGAG+AGG 0.500455 1_1:-56238440 None:intergenic
TGTAGGGAAATGCATCTTCT+GGG 0.504974 1_1:+56239869 Msa0030170:intron
GGATATTGAAACTCTGTCAA+TGG 0.511931 1_1:+56242248 Msa0030170:three_prime_UTR
CTTTCAGAGTGGGAGTTGGA+GGG 0.516133 1_1:+56238526 Msa0030170:CDS
ATCACCTGAGCAAACTGAAA+GGG 0.520857 1_1:+56241975 Msa0030170:exon
TAAATAACATTCACCTGTGA+AGG 0.522452 1_1:+56242117 Msa0030170:three_prime_UTR
TCCACCTGAGAACCATCATA+TGG 0.526072 1_1:-56242206 None:intergenic
ATGGCTTTCAGAGTGGGAGT+TGG 0.530306 1_1:+56238522 Msa0030170:CDS
TTTCAGAGTGGGAGTTGGAG+GGG 0.532929 1_1:+56238527 Msa0030170:CDS
ATAGCTATGGCTTTCAGAGT+GGG 0.535262 1_1:+56238516 Msa0030170:exon
CAGCAGCAGCTTCATAGCTA+TGG 0.536263 1_1:+56238503 Msa0030170:exon
GGGAAATGCATCTTCTGGGA+TGG 0.538512 1_1:+56239873 Msa0030170:intron
CATCAAAGGACAGGTTCAGA+AGG 0.548452 1_1:+56241869 Msa0030170:CDS
AGCTAAAATTCCTCTGCAGA+GGG 0.551878 1_1:-56242172 None:intergenic
GGAAATGCATCTTCTGGGAT+GGG 0.555903 1_1:+56239874 Msa0030170:CDS
GGCAACAGACCCAACAGAGA+TGG 0.557284 1_1:+56241921 Msa0030170:CDS
GATGGAACTCGAAGTCCTCC+AGG 0.560122 1_1:+56241939 Msa0030170:CDS
AAATCATCATAGCTCTCAGA+AGG 0.561770 1_1:-56240015 None:intergenic
GTGAAGGCTGATACTGATAT+GGG 0.562558 1_1:+56242133 Msa0030170:three_prime_UTR
GGACGAATACGAGCTACAGA+AGG 0.568172 1_1:-56241829 None:intergenic
CATAGCTATGGCTTTCAGAG+TGG 0.573358 1_1:+56238515 Msa0030170:exon
GAATTGTGTGGAGATGAAAA+GGG 0.573731 1_1:-56238408 None:intergenic
CTGTTGCCTGAATCTCATAG+TGG 0.585469 1_1:-56241906 None:intergenic
TTTATGCTACATCAAAGGAC+AGG 0.592719 1_1:+56241860 Msa0030170:CDS
ATATCAGTATCAGCCTTCAC+AGG 0.593659 1_1:-56242130 None:intergenic
GATGCTTTATGCTACATCAA+AGG 0.601779 1_1:+56241855 Msa0030170:CDS
CAGCTAAAATTCCTCTGCAG+AGG 0.602388 1_1:-56242173 None:intergenic
ACTCTGTCAATGGAGGTGTT+TGG 0.602656 1_1:+56242258 Msa0030170:three_prime_UTR
AATCACCTGAGCAAACTGAA+AGG 0.609728 1_1:+56241974 Msa0030170:exon
TTTGTTGAAAACATTTCTTG+AGG 0.612163 1_1:-56242026 None:intergenic
ATCAAAGGACAGGTTCAGAA+GGG 0.621100 1_1:+56241870 Msa0030170:CDS
CCTAAGAGACCCCTCTGCAG+AGG 0.626974 1_1:+56242162 Msa0030170:three_prime_UTR
GCCATATGATGGTTCTCAGG+TGG 0.634844 1_1:+56242205 Msa0030170:three_prime_UTR
GCACATTTCAGGAATTGCAG+AGG 0.661602 1_1:+56239917 Msa0030170:CDS
ATTAGTTCGCACGCTCCTGG+AGG 0.663424 1_1:-56241954 None:intergenic
GCTAAAATTCCTCTGCAGAG+GGG 0.687024 1_1:-56242171 None:intergenic
AGGTTCAGAAGGGAGCTGCA+GGG 0.689968 1_1:+56241880 Msa0030170:CDS
TATTGAAACTCTGTCAATGG+AGG 0.714240 1_1:+56242251 Msa0030170:three_prime_UTR
AATCATCATAGCTCTCAGAA+GGG 0.724970 1_1:-56240014 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGGTTAAAAATTATTTT+AGG - chr1_1:56238352-56238371 None:intergenic 10.0%
!! AAACATGATTGAAAAATATA+TGG - chr1_1:56238769-56238788 None:intergenic 15.0%
!!! ATTTGAATAAAATTTTCTGA+GGG + chr1_1:56238955-56238974 Msa0030170:intron 15.0%
!!! TAAAAAATGCTTTTGATATT+TGG + chr1_1:56240704-56240723 Msa0030170:intron 15.0%
!! ACACAAAAATGAATAAAAAA+AGG - chr1_1:56241395-56241414 None:intergenic 15.0%
!!! TTAATTAGTTTATATACTGT+AGG + chr1_1:56241434-56241453 Msa0030170:intron 15.0%
!! AAAAAATGATAGAATGAAGA+TGG - chr1_1:56238560-56238579 None:intergenic 20.0%
!!! CATTTGAATAAAATTTTCTG+AGG + chr1_1:56238954-56238973 Msa0030170:intron 20.0%
!! ACTTAATAATCATATCAAAG+GGG - chr1_1:56239289-56239308 None:intergenic 20.0%
!! CACTTAATAATCATATCAAA+GGG - chr1_1:56239290-56239309 None:intergenic 20.0%
!! ACACTTAATAATCATATCAA+AGG - chr1_1:56239291-56239310 None:intergenic 20.0%
!! TTGATATGATTATTAAGTGT+TGG + chr1_1:56239291-56239310 Msa0030170:intron 20.0%
!! TGATATGATTATTAAGTGTT+GGG + chr1_1:56239292-56239311 Msa0030170:intron 20.0%
!!! GATTATTTTGGTTTGAAAAA+AGG + chr1_1:56239812-56239831 Msa0030170:intron 20.0%
!!! TAAAAAAGATTTTGCTCTTT+TGG - chr1_1:56240113-56240132 None:intergenic 20.0%
!! ACATATGTATATATTGCATT+TGG + chr1_1:56240228-56240247 Msa0030170:intron 20.0%
!!! AAATAAAAAACCCTTTTAGA+AGG - chr1_1:56240775-56240794 None:intergenic 20.0%
!! AGACAAATATAATCAAGTAT+TGG - chr1_1:56240882-56240901 None:intergenic 20.0%
!! ATACTTGATTATATTTGTCT+CGG + chr1_1:56240882-56240901 Msa0030170:intron 20.0%
!! AATAATTCATATGAATGGAA+AGG - chr1_1:56240936-56240955 None:intergenic 20.0%
!! ATCACAATAATTCATATGAA+TGG - chr1_1:56240941-56240960 None:intergenic 20.0%
!! TTCATATGAATTATTGTGAT+AGG + chr1_1:56240941-56240960 Msa0030170:intron 20.0%
!!! TTTTGTGTTGATTTTTCTTT+TGG + chr1_1:56241407-56241426 Msa0030170:intron 20.0%
!!! TTTGTGTTGATTTTTCTTTT+GGG + chr1_1:56241408-56241427 Msa0030170:intron 20.0%
!! ATTTATATACATCAGAATCA+TGG + chr1_1:56241490-56241509 Msa0030170:intron 20.0%
!! GACAATCTTAAAAAAAACTT+TGG + chr1_1:56241523-56241542 Msa0030170:intron 20.0%
!!! TTTTATTTTCTGCTTACATA+TGG + chr1_1:56241658-56241677 Msa0030170:intron 20.0%
!! ACCATAATAATAGTACTTAA+GGG - chr1_1:56242054-56242073 None:intergenic 20.0%
!! AACCATAATAATAGTACTTA+AGG - chr1_1:56242055-56242074 None:intergenic 20.0%
!!! GAAGTAATATGCTTATTTTA+TGG + chr1_1:56242227-56242246 Msa0030170:three_prime_UTR 20.0%
!!! TTGTTTTTGTCGGATTTTAT+TGG + chr1_1:56238634-56238653 Msa0030170:intron 25.0%
!!! TCAGTTTTAATTTTGCTTTG+TGG + chr1_1:56238702-56238721 Msa0030170:intron 25.0%
!! AAACATATATCTTCGCATTT+TGG - chr1_1:56239030-56239049 None:intergenic 25.0%
! TCCAATGATGTTAAGAAATT+AGG + chr1_1:56239059-56239078 Msa0030170:intron 25.0%
! CATAGAAAGAATGCAAAAAT+GGG - chr1_1:56239172-56239191 None:intergenic 25.0%
! AAGTTAAATGTAGATAGTGT+AGG + chr1_1:56239384-56239403 Msa0030170:intron 25.0%
! GTATAAAGTGAGAAAATCAA+GGG - chr1_1:56239443-56239462 None:intergenic 25.0%
! AGTATAAAGTGAGAAAATCA+AGG - chr1_1:56239444-56239463 None:intergenic 25.0%
! ACTAAATCACCAATCAAAAA+GGG - chr1_1:56239635-56239654 None:intergenic 25.0%
! AACTAAATCACCAATCAAAA+AGG - chr1_1:56239636-56239655 None:intergenic 25.0%
!!! AGGACTTTAGTATTCTTTTT+TGG + chr1_1:56239782-56239801 Msa0030170:intron 25.0%
!!! GGACTTTAGTATTCTTTTTT+GGG + chr1_1:56239783-56239802 Msa0030170:intron 25.0%
!!! GACTTTAGTATTCTTTTTTG+GGG + chr1_1:56239784-56239803 Msa0030170:intron 25.0%
!!! ACTTTAGTATTCTTTTTTGG+GGG + chr1_1:56239785-56239804 Msa0030170:intron 25.0%
!!! TTTTGGTTTGAAAAAAGGAT+TGG + chr1_1:56239817-56239836 Msa0030170:intron 25.0%
! AATTTCGTGTAACTAATATG+TGG + chr1_1:56239840-56239859 Msa0030170:intron 25.0%
!!! TAATATGTGGATTTGTTGTA+GGG + chr1_1:56239853-56239872 Msa0030170:intron 25.0%
! AATTGATGAGAAAAAGAAAG+AGG + chr1_1:56239969-56239988 Msa0030170:CDS 25.0%
!! TTACTCAATTTTTCTTGCAT+TGG + chr1_1:56240186-56240205 Msa0030170:intron 25.0%
! AGCATATTGCTAATTTACTA+GGG + chr1_1:56240506-56240525 Msa0030170:intron 25.0%
!!! CTGGAATTTGAAATGATTTT+TGG - chr1_1:56240676-56240695 None:intergenic 25.0%
! AAACAACAATCACATGAATT+TGG - chr1_1:56240911-56240930 None:intergenic 25.0%
! TGATAAACTAATTGCCTATT+TGG - chr1_1:56240987-56241006 None:intergenic 25.0%
!!! TCTGATGTATTATTTCTGTT+TGG + chr1_1:56241158-56241177 Msa0030170:intron 25.0%
! CAATTGCAGAATATTATTGA+TGG - chr1_1:56241267-56241286 None:intergenic 25.0%
! ATACAGAATTAACAGTGATT+TGG - chr1_1:56241302-56241321 None:intergenic 25.0%
! TAATTCTGTATGTTTGGTAT+CGG + chr1_1:56241311-56241330 Msa0030170:intron 25.0%
!!! ATTTTTTTGCAATCCACTAT+TGG - chr1_1:56241565-56241584 None:intergenic 25.0%
! ATTATAGTATTGTAGCGTAA+CGG - chr1_1:56241605-56241624 None:intergenic 25.0%
! AGTAAACTCAAGCAAAATAA+AGG - chr1_1:56241785-56241804 None:intergenic 25.0%
!! ATTTTGCTTGAGTTTACTAA+TGG + chr1_1:56241787-56241806 Msa0030170:intron 25.0%
! TTTGTTGAAAACATTTCTTG+AGG - chr1_1:56242029-56242048 None:intergenic 25.0%
!! CATTTTTGCGAATTTAGCTT+GGG + chr1_1:56238367-56238386 Msa0030170:five_prime_UTR 30.0%
!!! ATTTTTGCGAATTTAGCTTG+GGG + chr1_1:56238368-56238387 Msa0030170:five_prime_UTR 30.0%
TGAAGAGTCAAGAATTTAGA+AGG - chr1_1:56238480-56238499 None:intergenic 30.0%
AAATCAAACCAAAATCAGCA+TGG - chr1_1:56238602-56238621 None:intergenic 30.0%
!!! TCGAATGTTGTTGTTTTTGT+CGG + chr1_1:56238624-56238643 Msa0030170:intron 30.0%
! TTCTTCCCACTACAAATTTT+GGG + chr1_1:56238677-56238696 Msa0030170:intron 30.0%
AAGAAAAACCGCATAATATG+AGG - chr1_1:56238734-56238753 None:intergenic 30.0%
GCCTAATTTCTTAACATCAT+TGG - chr1_1:56239063-56239082 None:intergenic 30.0%
TTGTCAAATGAAATCTTGTG+CGG + chr1_1:56239089-56239108 Msa0030170:intron 30.0%
! TTGAGTTACAAGTAGCTTTA+AGG + chr1_1:56239143-56239162 Msa0030170:intron 30.0%
CCATAGAAAGAATGCAAAAA+TGG - chr1_1:56239173-56239192 None:intergenic 30.0%
!! CCATTTTTGCATTCTTTCTA+TGG + chr1_1:56239170-56239189 Msa0030170:intron 30.0%
! TTATACTCTAGTCACTTTAG+AGG + chr1_1:56239457-56239476 Msa0030170:intron 30.0%
AGAAGTTTATGACTACACTT+CGG - chr1_1:56239491-56239510 None:intergenic 30.0%
ATGAGATATATCTTACTCCA+GGG - chr1_1:56239589-56239608 None:intergenic 30.0%
CTAAATCACCAATCAAAAAG+GGG - chr1_1:56239634-56239653 None:intergenic 30.0%
!! CTAATATGTGGATTTGTTGT+AGG + chr1_1:56239852-56239871 Msa0030170:intron 30.0%
! GCATATCTACAATCATTTTC+AGG - chr1_1:56240054-56240073 None:intergenic 30.0%
!!! GCAAAATCTTTTTTATCGCA+TGG + chr1_1:56240118-56240137 Msa0030170:CDS 30.0%
ATGCCTATTCTTAATCTTCA+TGG + chr1_1:56240418-56240437 Msa0030170:intron 30.0%
TGCCTATTCTTAATCTTCAT+GGG + chr1_1:56240419-56240438 Msa0030170:intron 30.0%
TATTCTTAATCTTCATGGGT+GGG + chr1_1:56240423-56240442 Msa0030170:intron 30.0%
GAGCATATTGCTAATTTACT+AGG + chr1_1:56240505-56240524 Msa0030170:intron 30.0%
!! CTGTATTTTTGAATGGAGTA+GGG + chr1_1:56240540-56240559 Msa0030170:intron 30.0%
!! TGTATTTTTGAATGGAGTAG+GGG + chr1_1:56240541-56240560 Msa0030170:intron 30.0%
!!! AGCATTTTTTATCAAGAGTC+TGG - chr1_1:56240695-56240714 None:intergenic 30.0%
! TGCTTTTGATATTTGGCAAA+AGG + chr1_1:56240711-56240730 Msa0030170:intron 30.0%
AACCAGATACTAAAAGAAAG+GGG - chr1_1:56240844-56240863 None:intergenic 30.0%
CAACCAGATACTAAAAGAAA+GGG - chr1_1:56240845-56240864 None:intergenic 30.0%
ACAACCAGATACTAAAAGAA+AGG - chr1_1:56240846-56240865 None:intergenic 30.0%
TGTTAGAAGAATGTAGGAAA+CGG + chr1_1:56241036-56241055 Msa0030170:intron 30.0%
CACTGTTAATTCTGTATGTT+TGG + chr1_1:56241305-56241324 Msa0030170:intron 30.0%
!! AGTGGATTGCAAAAAAATAG+CGG + chr1_1:56241567-56241586 Msa0030170:intron 30.0%
! CTAATGGATAATTGCTTTAC+AGG + chr1_1:56241803-56241822 Msa0030170:intron 30.0%
AAGGGTTATGTCTATTAGTT+AGG + chr1_1:56241993-56242012 Msa0030170:three_prime_UTR 30.0%
GCCCTTAAGTACTATTATTA+TGG + chr1_1:56242050-56242069 Msa0030170:three_prime_UTR 30.0%
TAAATAACATTCACCTGTGA+AGG + chr1_1:56242117-56242136 Msa0030170:three_prime_UTR 30.0%
GAACTAGAAAAATACGAAAC+AGG - chr1_1:56242300-56242319 None:intergenic 30.0%
CCAAGCTAAATTCGCAAAAA+TGG - chr1_1:56238369-56238388 None:intergenic 35.0%
!! CCATTTTTGCGAATTTAGCT+TGG + chr1_1:56238366-56238385 Msa0030170:five_prime_UTR 35.0%
!!! TTTTTGCGAATTTAGCTTGG+GGG + chr1_1:56238369-56238388 Msa0030170:five_prime_UTR 35.0%
GAATTGTGTGGAGATGAAAA+GGG - chr1_1:56238411-56238430 None:intergenic 35.0%
AGAATTGTGTGGAGATGAAA+AGG - chr1_1:56238412-56238431 None:intergenic 35.0%
! GTTCTTCCCACTACAAATTT+TGG + chr1_1:56238676-56238695 Msa0030170:intron 35.0%
TGAGACCCAAAATTTGTAGT+GGG - chr1_1:56238685-56238704 None:intergenic 35.0%
! GGTTGATTCCTCATATTATG+CGG + chr1_1:56238723-56238742 Msa0030170:intron 35.0%
GCATGTCTTTAGTACTTTCT+TGG + chr1_1:56238977-56238996 Msa0030170:intron 35.0%
TCTTTAGTACTTTCTTGGAC+AGG + chr1_1:56238982-56239001 Msa0030170:intron 35.0%
!! GATTATTAAGTGTTGGGATC+CGG + chr1_1:56239298-56239317 Msa0030170:intron 35.0%
TTATGACTACACTTCGGATT+TGG - chr1_1:56239485-56239504 None:intergenic 35.0%
CATGAGATATATCTTACTCC+AGG - chr1_1:56239590-56239609 None:intergenic 35.0%
! TACAGTTTCCCCTTTTTGAT+TGG + chr1_1:56239623-56239642 Msa0030170:intron 35.0%
AATGAGGAAAACAGCATGTT+TGG - chr1_1:56239741-56239760 None:intergenic 35.0%
CTGGGATTCATTTCATTGAA+AGG + chr1_1:56239762-56239781 Msa0030170:intron 35.0%
!! TTTGGGGGAGTTGATTATTT+TGG + chr1_1:56239800-56239819 Msa0030170:intron 35.0%
TGAGAAAAAGAAAGAGGTTG+TGG + chr1_1:56239975-56239994 Msa0030170:CDS 35.0%
AATCATCATAGCTCTCAGAA+GGG - chr1_1:56240017-56240036 None:intergenic 35.0%
AAATCATCATAGCTCTCAGA+AGG - chr1_1:56240018-56240037 None:intergenic 35.0%
!! TCTACAATCATTTTCAGGCA+AGG - chr1_1:56240049-56240068 None:intergenic 35.0%
AAAGACAGAAATCTGAACGA+GGG + chr1_1:56240305-56240324 Msa0030170:intron 35.0%
CACCCATGAAGATTAAGAAT+AGG - chr1_1:56240424-56240443 None:intergenic 35.0%
CTATTCTTAATCTTCATGGG+TGG + chr1_1:56240422-56240441 Msa0030170:intron 35.0%
!! TGCCAAGCTGTATTTTTGAA+TGG + chr1_1:56240533-56240552 Msa0030170:intron 35.0%
CTCCATTCAAAAATACAGCT+TGG - chr1_1:56240538-56240557 None:intergenic 35.0%
!! GCTGTATTTTTGAATGGAGT+AGG + chr1_1:56240539-56240558 Msa0030170:intron 35.0%
ACTATAGTACCTTGTTGAAC+TGG + chr1_1:56240621-56240640 Msa0030170:intron 35.0%
CTATAGTACCTTGTTGAACT+GGG + chr1_1:56240622-56240641 Msa0030170:intron 35.0%
TATAGTACCTTGTTGAACTG+GGG + chr1_1:56240623-56240642 Msa0030170:intron 35.0%
GTTTATGCAAACGGTTCAAA+CGG - chr1_1:56240746-56240765 None:intergenic 35.0%
TAAACCCATAGCCTTCTAAA+AGG + chr1_1:56240761-56240780 Msa0030170:intron 35.0%
! AAACCCATAGCCTTCTAAAA+GGG + chr1_1:56240762-56240781 Msa0030170:intron 35.0%
!! AAACCCTTTTAGAAGGCTAT+GGG - chr1_1:56240768-56240787 None:intergenic 35.0%
!! AAAACCCTTTTAGAAGGCTA+TGG - chr1_1:56240769-56240788 None:intergenic 35.0%
! ACAACCTTGCTATAATCAAC+TGG + chr1_1:56240798-56240817 Msa0030170:intron 35.0%
CATACCAGTTGATTATAGCA+AGG - chr1_1:56240805-56240824 None:intergenic 35.0%
TGAATTATTGTGATAGGACC+CGG + chr1_1:56240947-56240966 Msa0030170:intron 35.0%
GATGAGAATGTGATCCAAAT+AGG + chr1_1:56240970-56240989 Msa0030170:intron 35.0%
GTGTGATGTTAGAAGAATGT+AGG + chr1_1:56241030-56241049 Msa0030170:intron 35.0%
GAAAATAATGTGACGTGGTA+GGG + chr1_1:56241062-56241081 Msa0030170:intron 35.0%
AAAATAATGTGACGTGGTAG+GGG + chr1_1:56241063-56241082 Msa0030170:intron 35.0%
! TGTGACCATCTTGTTATAAG+CGG + chr1_1:56241107-56241126 Msa0030170:intron 35.0%
TCAGAGAAAATACACTTCTC+TGG - chr1_1:56241184-56241203 None:intergenic 35.0%
!! AGAAGTGTATTTTCTCTGAG+AGG + chr1_1:56241185-56241204 Msa0030170:intron 35.0%
ACGAAAGTAGATTGCAGAAA+GGG - chr1_1:56241242-56241261 None:intergenic 35.0%
AACGAAAGTAGATTGCAGAA+AGG - chr1_1:56241243-56241262 None:intergenic 35.0%
ATACCATATATGTAGCCACT+GGG - chr1_1:56241355-56241374 None:intergenic 35.0%
TTTATATACTGTAGGCATCC+TGG + chr1_1:56241442-56241461 Msa0030170:intron 35.0%
TAAACTAGTGTTGCCAATAG+TGG + chr1_1:56241549-56241568 Msa0030170:intron 35.0%
TTCTCTGCAAGCAGATAATA+GGG - chr1_1:56241630-56241649 None:intergenic 35.0%
TTTCTCTGCAAGCAGATAAT+AGG - chr1_1:56241631-56241650 None:intergenic 35.0%
! TTTTCTGCTTACATATGGAG+AGG + chr1_1:56241663-56241682 Msa0030170:intron 35.0%
AACGCAACATGGTAAATGAA+AGG - chr1_1:56241724-56241743 None:intergenic 35.0%
TGTCAAATTACAACTTCCTC+TGG - chr1_1:56241750-56241769 None:intergenic 35.0%
! GATGTAGCATAAAGCATCTT+CGG - chr1_1:56241853-56241872 None:intergenic 35.0%
GATGCTTTATGCTACATCAA+AGG + chr1_1:56241855-56241874 Msa0030170:CDS 35.0%
TTTATGCTACATCAAAGGAC+AGG + chr1_1:56241860-56241879 Msa0030170:CDS 35.0%
TGTTCCTTATTGCCATATGA+TGG + chr1_1:56242194-56242213 Msa0030170:three_prime_UTR 35.0%
AGAACCATCATATGGCAATA+AGG - chr1_1:56242201-56242220 None:intergenic 35.0%
GGATATTGAAACTCTGTCAA+TGG + chr1_1:56242248-56242267 Msa0030170:three_prime_UTR 35.0%
TATTGAAACTCTGTCAATGG+AGG + chr1_1:56242251-56242270 Msa0030170:three_prime_UTR 35.0%
ATAGCTATGGCTTTCAGAGT+GGG + chr1_1:56238516-56238535 Msa0030170:exon 40.0%
! CCAAAATCAGCATGGTCTTA+AGG - chr1_1:56238594-56238613 None:intergenic 40.0%
!!! CCTTAAGACCATGCTGATTT+TGG + chr1_1:56238591-56238610 Msa0030170:intron 40.0%
!! GTCGGATTTTATTGGTTGCA+TGG + chr1_1:56238642-56238661 Msa0030170:intron 40.0%
CTGAGACCCAAAATTTGTAG+TGG - chr1_1:56238686-56238705 None:intergenic 40.0%
TCAGCATTGTCAGTTGAGAT+AGG - chr1_1:56238824-56238843 None:intergenic 40.0%
TCAAAGCAACTTCATGCATG+AGG - chr1_1:56239127-56239146 None:intergenic 40.0%
CTTATTACTAGCGTCAGTGT+CGG - chr1_1:56239234-56239253 None:intergenic 40.0%
AAGTGAATTGCCCACTTTAG+AGG - chr1_1:56239326-56239345 None:intergenic 40.0%
GTAAGATATATCTCATGCCC+CGG + chr1_1:56239593-56239612 Msa0030170:intron 40.0%
TAAGATATATCTCATGCCCC+GGG + chr1_1:56239594-56239613 Msa0030170:intron 40.0%
GGAAACTGTAAATATTCCCG+GGG - chr1_1:56239613-56239632 None:intergenic 40.0%
GGGAAACTGTAAATATTCCC+GGG - chr1_1:56239614-56239633 None:intergenic 40.0%
GGGGAAACTGTAAATATTCC+CGG - chr1_1:56239615-56239634 None:intergenic 40.0%
!! TGTTTGGCAGAGCATTCTTT+TGG - chr1_1:56239725-56239744 None:intergenic 40.0%
!!! ATGCTGTTTTCCTCATTGCT+GGG + chr1_1:56239744-56239763 Msa0030170:intron 40.0%
TGAAATGAATCCCAGCAATG+AGG - chr1_1:56239757-56239776 None:intergenic 40.0%
TTGTAGGGAAATGCATCTTC+TGG + chr1_1:56239868-56239887 Msa0030170:intron 40.0%
TGTAGGGAAATGCATCTTCT+GGG + chr1_1:56239869-56239888 Msa0030170:intron 40.0%
CTCGTTCAGATTTCTGTCTT+TGG - chr1_1:56240306-56240325 None:intergenic 40.0%
CAAAGACAGAAATCTGAACG+AGG + chr1_1:56240304-56240323 Msa0030170:intron 40.0%
AGGCTATGGGTTTATGCAAA+CGG - chr1_1:56240755-56240774 None:intergenic 40.0%
! GACCCCTTTCTTTTAGTATC+TGG + chr1_1:56240839-56240858 Msa0030170:intron 40.0%
GAATTATTGTGATAGGACCC+GGG + chr1_1:56240948-56240967 Msa0030170:intron 40.0%
TTTGGATCACATTCTCATCC+CGG - chr1_1:56240969-56240988 None:intergenic 40.0%
AGTTGTCCAAATCCATTGTG+TGG + chr1_1:56241006-56241025 Msa0030170:intron 40.0%
GTTGTCCAAATCCATTGTGT+GGG + chr1_1:56241007-56241026 Msa0030170:intron 40.0%
TTGTCCAAATCCATTGTGTG+GGG + chr1_1:56241008-56241027 Msa0030170:intron 40.0%
TAGAAGAATGTAGGAAACGG+AGG + chr1_1:56241039-56241058 Msa0030170:intron 40.0%
AGAAGAATGTAGGAAACGGA+GGG + chr1_1:56241040-56241059 Msa0030170:intron 40.0%
GGAAAATAATGTGACGTGGT+AGG + chr1_1:56241061-56241080 Msa0030170:intron 40.0%
! GTGACCATCTTGTTATAAGC+GGG + chr1_1:56241108-56241127 Msa0030170:intron 40.0%
TATCCCAGTGGCTACATATA+TGG + chr1_1:56241349-56241368 Msa0030170:intron 40.0%
GATACCATATATGTAGCCAC+TGG - chr1_1:56241356-56241375 None:intergenic 40.0%
GCAAAGTGTGAAGATAGAGA+TGG - chr1_1:56241689-56241708 None:intergenic 40.0%
ATCAAAGGACAGGTTCAGAA+GGG + chr1_1:56241870-56241889 Msa0030170:CDS 40.0%
AATCACCTGAGCAAACTGAA+AGG + chr1_1:56241974-56241993 Msa0030170:exon 40.0%
ATCACCTGAGCAAACTGAAA+GGG + chr1_1:56241975-56241994 Msa0030170:exon 40.0%
! ATAACCCTTTCAGTTTGCTC+AGG - chr1_1:56241982-56242001 None:intergenic 40.0%
ATATCAGTATCAGCCTTCAC+AGG - chr1_1:56242133-56242152 None:intergenic 40.0%
TGTGAAGGCTGATACTGATA+TGG + chr1_1:56242132-56242151 Msa0030170:three_prime_UTR 40.0%
GTGAAGGCTGATACTGATAT+GGG + chr1_1:56242133-56242152 Msa0030170:three_prime_UTR 40.0%
AGCTAAAATTCCTCTGCAGA+GGG - chr1_1:56242175-56242194 None:intergenic 40.0%
ATTGCCATATGATGGTTCTC+AGG + chr1_1:56242202-56242221 Msa0030170:three_prime_UTR 40.0%
AGGAGAGAGAGAGAATTGTG+TGG - chr1_1:56238423-56238442 None:intergenic 45.0%
AAAGTGAGAGTGAGAGTGAG+AGG - chr1_1:56238443-56238462 None:intergenic 45.0%
CATAGCTATGGCTTTCAGAG+TGG + chr1_1:56238515-56238534 Msa0030170:exon 45.0%
AACCTGTGAAGCATAGACAC+TGG + chr1_1:56239200-56239219 Msa0030170:intron 45.0%
! ATTGCCCACTTTAGAGGATC+CGG - chr1_1:56239320-56239339 None:intergenic 45.0%
! TACATGCACATTTTGTGCCC+TGG + chr1_1:56239569-56239588 Msa0030170:intron 45.0%
!!! CATGCTGTTTTCCTCATTGC+TGG + chr1_1:56239743-56239762 Msa0030170:intron 45.0%
!! GGAAATGCATCTTCTGGGAT+GGG + chr1_1:56239874-56239893 Msa0030170:CDS 45.0%
GCACATTTCAGGAATTGCAG+AGG + chr1_1:56239917-56239936 Msa0030170:CDS 45.0%
GAGGTTGTGGTCGAAAAAAC+TGG + chr1_1:56239988-56240007 Msa0030170:CDS 45.0%
GTTGTGGTCGAAAAAACTGG+AGG + chr1_1:56239991-56240010 Msa0030170:CDS 45.0%
TTGGATCACATTCTCATCCC+GGG - chr1_1:56240968-56240987 None:intergenic 45.0%
TAACATCACACCCCACACAA+TGG - chr1_1:56241021-56241040 None:intergenic 45.0%
GGAGGGAAAATAATGTGACG+TGG + chr1_1:56241057-56241076 Msa0030170:intron 45.0%
CCTACCCGCTTATAACAAGA+TGG - chr1_1:56241115-56241134 None:intergenic 45.0%
! CCATCTTGTTATAAGCGGGT+AGG + chr1_1:56241112-56241131 Msa0030170:intron 45.0%
! TCTTGTTATAAGCGGGTAGG+AGG + chr1_1:56241115-56241134 Msa0030170:intron 45.0%
AGATTGTCGATCTTGAGACC+AGG - chr1_1:56241463-56241482 None:intergenic 45.0%
ACCATGTTGCGTTAAACCAG+AGG + chr1_1:56241731-56241750 Msa0030170:intron 45.0%
! TCCTCTGGTTTAACGCAACA+TGG - chr1_1:56241735-56241754 None:intergenic 45.0%
CATCAAAGGACAGGTTCAGA+AGG + chr1_1:56241869-56241888 Msa0030170:CDS 45.0%
GGGAATCCACTATGAGATTC+AGG + chr1_1:56241900-56241919 Msa0030170:CDS 45.0%
CTGTTGCCTGAATCTCATAG+TGG - chr1_1:56241909-56241928 None:intergenic 45.0%
! CTTCGAGTTCCATCTCTGTT+GGG - chr1_1:56241933-56241952 None:intergenic 45.0%
! ACTTCGAGTTCCATCTCTGT+TGG - chr1_1:56241934-56241953 None:intergenic 45.0%
GCTAAAATTCCTCTGCAGAG+GGG - chr1_1:56242174-56242193 None:intergenic 45.0%
CAGCTAAAATTCCTCTGCAG+AGG - chr1_1:56242176-56242195 None:intergenic 45.0%
TCCACCTGAGAACCATCATA+TGG - chr1_1:56242209-56242228 None:intergenic 45.0%
!! ACTCTGTCAATGGAGGTGTT+TGG + chr1_1:56242258-56242277 Msa0030170:three_prime_UTR 45.0%
CAGCAGCAGCTTCATAGCTA+TGG + chr1_1:56238503-56238522 Msa0030170:exon 50.0%
ATGGCTTTCAGAGTGGGAGT+TGG + chr1_1:56238522-56238541 Msa0030170:CDS 50.0%
CTTTCAGAGTGGGAGTTGGA+GGG + chr1_1:56238526-56238545 Msa0030170:CDS 50.0%
TTTCAGAGTGGGAGTTGGAG+GGG + chr1_1:56238527-56238546 Msa0030170:CDS 50.0%
GTGTGTTAGACCCTTTCGCT+CGG + chr1_1:56238848-56238867 Msa0030170:intron 50.0%
ACGATCGTTTCCGAGCGAAA+GGG - chr1_1:56238861-56238880 None:intergenic 50.0%
GTCCAGTGTCTATGCTTCAC+AGG - chr1_1:56239205-56239224 None:intergenic 50.0%
!! GGATCCGGATCCTCTAAAGT+GGG + chr1_1:56239313-56239332 Msa0030170:intron 50.0%
! GGGAAATGCATCTTCTGGGA+TGG + chr1_1:56239873-56239892 Msa0030170:intron 50.0%
GCAGAGCGTAAGCACATTTC+AGG + chr1_1:56239906-56239925 Msa0030170:CDS 50.0%
ATGCAGTCCCCAGTTCAACA+AGG - chr1_1:56240633-56240652 None:intergenic 50.0%
! CACACCCCACACAATGGATT+TGG - chr1_1:56241015-56241034 None:intergenic 50.0%
TAATGTGACGTGGTAGGGGA+TGG + chr1_1:56241067-56241086 Msa0030170:intron 50.0%
AATGTGACGTGGTAGGGGAT+GGG + chr1_1:56241068-56241087 Msa0030170:intron 50.0%
TGTTATAAGCGGGTAGGAGG+AGG + chr1_1:56241118-56241137 Msa0030170:intron 50.0%
GTTATAAGCGGGTAGGAGGA+GGG + chr1_1:56241119-56241138 Msa0030170:intron 50.0%
TGAGAGGAGCCTAGCTATGT+GGG + chr1_1:56241201-56241220 Msa0030170:intron 50.0%
CGCATCATACTGTATCCCAG+TGG + chr1_1:56241337-56241356 Msa0030170:intron 50.0%
GGACGAATACGAGCTACAGA+AGG - chr1_1:56241832-56241851 None:intergenic 50.0%
GCCATATGATGGTTCTCAGG+TGG + chr1_1:56242205-56242224 Msa0030170:three_prime_UTR 50.0%
!! GCGAATTTAGCTTGGGGGAC+TGG + chr1_1:56238374-56238393 Msa0030170:five_prime_UTR 55.0%
GCTTTCAGAGTGGGAGTTGG+AGG + chr1_1:56238525-56238544 Msa0030170:CDS 55.0%
CACGATCGTTTCCGAGCGAA+AGG - chr1_1:56238862-56238881 None:intergenic 55.0%
! GGGATCCGGATCCTCTAAAG+TGG + chr1_1:56239312-56239331 Msa0030170:intron 55.0%
ATGTGACGTGGTAGGGGATG+GGG + chr1_1:56241069-56241088 Msa0030170:intron 55.0%
CTGAGAGGAGCCTAGCTATG+TGG + chr1_1:56241200-56241219 Msa0030170:intron 55.0%
GAGCCTAGCTATGTGGGTAG+AGG + chr1_1:56241207-56241226 Msa0030170:intron 55.0%
CCTCCTCTACCCACATAGCT+AGG - chr1_1:56241213-56241232 None:intergenic 55.0%
CCTAGCTATGTGGGTAGAGG+AGG + chr1_1:56241210-56241229 Msa0030170:intron 55.0%
AGGTTCAGAAGGGAGCTGCA+GGG + chr1_1:56241880-56241899 Msa0030170:CDS 55.0%
GGCAACAGACCCAACAGAGA+TGG + chr1_1:56241921-56241940 Msa0030170:CDS 55.0%
GATGGAACTCGAAGTCCTCC+AGG + chr1_1:56241939-56241958 Msa0030170:CDS 55.0%
ATTAGTTCGCACGCTCCTGG+AGG - chr1_1:56241957-56241976 None:intergenic 55.0%
! GTGATTAGTTCGCACGCTCC+TGG - chr1_1:56241960-56241979 None:intergenic 55.0%
GGGTAGGAGGAGGGAGAGAA+AGG + chr1_1:56241128-56241147 Msa0030170:intron 60.0%
CAGGTTCAGAAGGGAGCTGC+AGG + chr1_1:56241879-56241898 Msa0030170:CDS 60.0%
CCTCTGCAGAGGGGTCTCTT+AGG - chr1_1:56242165-56242184 None:intergenic 60.0%
CCTAAGAGACCCCTCTGCAG+AGG + chr1_1:56242162-56242181 Msa0030170:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 56238343 56242336 56238343 ID=Msa0030170;Name=Msa0030170
chr1_1 mRNA 56238343 56242336 56238343 ID=Msa0030170-mRNA-1;Parent=Msa0030170;Name=Msa0030170-mRNA-1;_AED=0.02;_eAED=0.02;_QI=179|1|1|1|1|1|3|361|147
chr1_1 exon 56238343 56238548 56238343 ID=Msa0030170-mRNA-1:exon:16786;Parent=Msa0030170-mRNA-1
chr1_1 exon 56239874 56240139 56239874 ID=Msa0030170-mRNA-1:exon:16787;Parent=Msa0030170-mRNA-1
chr1_1 exon 56241825 56242336 56241825 ID=Msa0030170-mRNA-1:exon:16788;Parent=Msa0030170-mRNA-1
chr1_1 five_prime_UTR 56238343 56238521 56238343 ID=Msa0030170-mRNA-1:five_prime_utr;Parent=Msa0030170-mRNA-1
chr1_1 CDS 56238522 56238548 56238522 ID=Msa0030170-mRNA-1:cds;Parent=Msa0030170-mRNA-1
chr1_1 CDS 56239874 56240139 56239874 ID=Msa0030170-mRNA-1:cds;Parent=Msa0030170-mRNA-1
chr1_1 CDS 56241825 56241975 56241825 ID=Msa0030170-mRNA-1:cds;Parent=Msa0030170-mRNA-1
chr1_1 three_prime_UTR 56241976 56242336 56241976 ID=Msa0030170-mRNA-1:three_prime_utr;Parent=Msa0030170-mRNA-1
Gene Sequence

>Msa0030170

ATGGCTTTCAGAGTGGGAGTTGGAGGGGGAAATGCATCTTCTGGGATGGGTGTTGCTGAGCAGAGCGTAAGCACATTTCAGGAATTGCAGAGGAAGAAAGTATATCGCTATGTGATTTTCAAAATTGATGAGAAAAAGAAAGAGGTTGTGGTCGAAAAAACTGGAGGCCCTTCTGAGAGCTATGATGATTTCACTGCATCCTTGCCTGAAAATGATTGTAGATATGCAGTCTTTGACTTTGATTTCGTGACTGCTGAGAACTGCCAAAAGAGCAAAATCTTTTTTATCGCATGGTCTCCTTCTGTAGCTCGTATTCGTCCGAAGATGCTTTATGCTACATCAAAGGACAGGTTCAGAAGGGAGCTGCAGGGAATCCACTATGAGATTCAGGCAACAGACCCAACAGAGATGGAACTCGAAGTCCTCCAGGAGCGTGCGAACTAA
Protein sequence

>Msa0030170

MAFRVGVGGGNASSGMGVAEQSVSTFQELQRKKVYRYVIFKIDEKKKEVVVEKTGGPSESYDDFTASLPENDCRYAVFDFDFVTAENCQKSKIFFIAWSPSVARIRPKMLYATSKDRFRRELQGIHYEIQATDPTEMELEVLQERAN*