AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0079030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0079030 MtrunA17_Chr4g0047001 80.220 91 9 2 50 131 276 366 3.50e-35 126
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 116 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGATTCTTCTTCTCTTTAT+TGG 0.115189 1_2:-56725061 Msa0079030:CDS
TCAAAATCAGATGGAGAATA+TGG 0.162268 1_2:-56725090 Msa0079030:CDS
TGTGGGATTGGTTTGGTATT+TGG 0.216703 1_2:-56724535 Msa0079030:three_prime_UTR
GGGTTAGGTTATGGGGTTCT+TGG 0.239585 1_2:-56724627 Msa0079030:three_prime_UTR
ATTTGGAAGGGGTTAGGTTA+TGG 0.258152 1_2:-56724636 Msa0079030:three_prime_UTR
ATTTATTATTGTGATTAGTT+AGG 0.275581 1_2:-56724789 Msa0079030:three_prime_UTR
CAACAGGTTTGAGCATTGTT+TGG 0.276112 1_2:-56725007 Msa0079030:CDS
TAATATTATATACTTTGATT+TGG 0.287818 1_2:-56724572 Msa0079030:three_prime_UTR
TTTGGAAGGGGTTAGGTTAT+GGG 0.300637 1_2:-56724635 Msa0079030:three_prime_UTR
GACAACACACATATATAAAA+AGG 0.303432 1_2:+56723928 None:intergenic
GGAGTATGAATATTAGAAAT+TGG 0.313771 1_2:+56724190 None:intergenic
CACCTAGCACTATAGTTTCT+AGG 0.314429 1_2:+56724248 None:intergenic
ATGTGAAATCTCCAAAAGTT+AGG 0.325722 1_2:+56723965 None:intergenic
TCATAATTACTGCAAGAAAA+AGG 0.336420 1_2:+56725447 None:intergenic
TGTGTTGTCAACTTTGTTCA+TGG 0.339702 1_2:-56723914 Msa0079030:three_prime_UTR
AAATTGTTGCAGAGAATATA+AGG 0.356821 1_2:+56723115 None:intergenic
CTTAGCATTTGGAAGGGGTT+AGG 0.361045 1_2:-56724642 Msa0079030:three_prime_UTR
GGATATGGAGGTTCTTCATT+TGG 0.372923 1_2:-56724916 Msa0079030:CDS
TCAAAACTGTTACACACAAT+AGG 0.374921 1_2:-56724351 Msa0079030:three_prime_UTR
AACAGGTTTGAGCATTGTTT+GGG 0.378741 1_2:-56725006 Msa0079030:CDS
CAAACTTATATGCTGTCATT+TGG 0.391874 1_2:-56724310 Msa0079030:three_prime_UTR
TATAATTGTGGTATAGAAAA+GGG 0.395043 1_2:-56724601 Msa0079030:three_prime_UTR
ATTAGTTAGGGTGGTGAGGT+TGG 0.396408 1_2:-56724776 Msa0079030:three_prime_UTR
TTTATTATTGTGATTAGTTA+GGG 0.397649 1_2:-56724788 Msa0079030:three_prime_UTR
GGAGCTCAGATCAAGGGATA+TGG 0.400830 1_2:-56724931 Msa0079030:CDS
TTGCATTAAGGCAAGTATCT+TGG 0.400860 1_2:+56724050 None:intergenic
AACAACAACAACACATCAAC+AGG 0.406264 1_2:-56725023 Msa0079030:CDS
GGCAAGTATCTTGGTTCTGA+TGG 0.414846 1_2:+56724059 None:intergenic
CGATTTACTTTGTGATCACT+TGG 0.419587 1_2:-56723858 Msa0079030:three_prime_UTR
CAAAATCAGATGGAGAATAT+GGG 0.427482 1_2:-56725089 Msa0079030:CDS
TTGTTAACTTCCTTAGCATT+TGG 0.428514 1_2:-56724653 Msa0079030:three_prime_UTR
CAAAACTGTTACACACAATA+GGG 0.431384 1_2:-56724350 Msa0079030:three_prime_UTR
GATTAATATGTATGTGGGAT+TGG 0.432302 1_2:-56724547 Msa0079030:three_prime_UTR
GGTGGTGGTGGTGGAATGAA+AGG 0.434012 1_2:-56725236 Msa0079030:CDS
ATATGCTGTCATTTGGTGGC+AGG 0.434732 1_2:-56724303 Msa0079030:three_prime_UTR
AGTTAGGGTGGTGAGGTTGG+TGG 0.436068 1_2:-56724773 Msa0079030:three_prime_UTR
GATGAAAGGAAATTTAAAGT+TGG 0.437503 1_2:-56724735 Msa0079030:three_prime_UTR
GTATGTGGGCTATGGTAGTC+TGG 0.441008 1_2:-56724375 Msa0079030:three_prime_UTR
ACAGTTGCTGGTGGTGGTGG+TGG 0.441112 1_2:-56725251 Msa0079030:intron
CAGATGGGACAAACCTTGAT+AGG 0.444277 1_2:+56725496 None:intergenic
CAATTTGGAATATCCTATCA+AGG 0.446034 1_2:-56725509 Msa0079030:CDS
GTATAATTGTGGTATAGAAA+AGG 0.452141 1_2:-56724602 Msa0079030:three_prime_UTR
TCATCATGAGGCTGGAATGA+TGG 0.453701 1_2:-56725201 Msa0079030:CDS
TAATAAAAGCATATAGTCAT+TGG 0.458369 1_2:+56723889 None:intergenic
TAGGTGAAGGACTACAAAAT+AGG 0.461463 1_2:-56725160 Msa0079030:CDS
TATTCAATGCATCATTAGAC+AGG 0.463699 1_2:-56724124 Msa0079030:three_prime_UTR
AACCAACACATTAGTTCCTT+AGG 0.465971 1_2:+56724682 None:intergenic
TGTATTATCATTAGTTCCTA+AGG 0.478740 1_2:-56724698 Msa0079030:three_prime_UTR
AAGTAAAAGATTGAATTTGA+AGG 0.479107 1_2:-56723800 Msa0079030:three_prime_UTR
GACATAGTTGAATATACATA+AGG 0.481229 1_2:+56723170 None:intergenic
CCATTTCGAATCTATATGCT+TGG 0.484477 1_2:+56724407 None:intergenic
AGTACAGTTGCTGGTGGTGG+TGG 0.486906 1_2:-56725254 Msa0079030:intron
CCAAGCATATAGATTCGAAA+TGG 0.488060 1_2:-56724407 Msa0079030:three_prime_UTR
ATTCCAGCCTCATGATGAAA+AGG 0.488849 1_2:+56725206 None:intergenic
ATAGTGAATAACTTGCATTA+AGG 0.489444 1_2:+56724038 None:intergenic
TGTCTGTGGAGCTCAGATCA+AGG 0.492001 1_2:-56724938 Msa0079030:CDS
TGATGAGAGAAGAGGATTTG+AGG 0.498919 1_2:+56724973 None:intergenic
TTGGTTTGGTATTTGGTGTG+TGG 0.501927 1_2:-56724528 Msa0079030:three_prime_UTR
TGTGAAATCTCCAAAAGTTA+GGG 0.502739 1_2:+56723966 None:intergenic
AAATGGTGTGGAACTGTATG+TGG 0.507090 1_2:-56724390 Msa0079030:three_prime_UTR
AAACTATAGTGCTAGGTGAC+AGG 0.508739 1_2:-56724243 Msa0079030:three_prime_UTR
ATATGTATGTGGGATTGGTT+TGG 0.509849 1_2:-56724542 Msa0079030:three_prime_UTR
TTGGATGATTAATATGTATG+TGG 0.510167 1_2:-56724553 Msa0079030:three_prime_UTR
AATGTAAAATGAGAACATCT+TGG 0.510570 1_2:-56723215 Msa0079030:three_prime_UTR
GCATCATTAGACAGGGGTAA+AGG 0.511273 1_2:-56724116 Msa0079030:three_prime_UTR
TTCCTAAGGAACTAATGTGT+TGG 0.513830 1_2:-56724684 Msa0079030:three_prime_UTR
ATGAGTACAGTTGCTGGTGG+TGG 0.517838 1_2:-56725257 Msa0079030:intron
TGAAGGACTACAAAATAGGC+TGG 0.519058 1_2:-56725156 Msa0079030:CDS
ACTTATATGCTGTCATTTGG+TGG 0.520104 1_2:-56724307 Msa0079030:three_prime_UTR
AGTTGATTGATTTAGTAAGT+TGG 0.524122 1_2:-56724878 Msa0079030:three_prime_UTR
TAACTTCCTTAGCATTTGGA+AGG 0.525002 1_2:-56724649 Msa0079030:three_prime_UTR
TGTGATTAGTTAGGGTGGTG+AGG 0.530440 1_2:-56724780 Msa0079030:three_prime_UTR
TTGGAAGGGGTTAGGTTATG+GGG 0.530517 1_2:-56724634 Msa0079030:three_prime_UTR
AACTTCCTTAGCATTTGGAA+GGG 0.531451 1_2:-56724648 Msa0079030:three_prime_UTR
AGTAATGATATGAATGCTAA+TGG 0.543549 1_2:-56725125 Msa0079030:CDS
ATTATTGTGATTAGTTAGGG+TGG 0.544877 1_2:-56724785 Msa0079030:three_prime_UTR
TGGATGATTAATATGTATGT+GGG 0.548565 1_2:-56724552 Msa0079030:three_prime_UTR
GTGGAACTGTATGTGGGCTA+TGG 0.548758 1_2:-56724383 Msa0079030:three_prime_UTR
TGACATGAAGATAGAAGAGA+TGG 0.552077 1_2:+56724009 None:intergenic
AGTGAAATAAAAGATGCAAG+AGG 0.555359 1_2:+56724169 None:intergenic
TATGCTGTCATTTGGTGGCA+GGG 0.557588 1_2:-56724302 Msa0079030:three_prime_UTR
CTTACTAAATCAATCAACTA+CGG 0.562658 1_2:+56724882 None:intergenic
GATTCAAAAGAAGTGAAAGT+AGG 0.562786 1_2:-56725179 Msa0079030:CDS
TGGTTCTGATGGGAATCTCA+AGG 0.563170 1_2:+56724070 None:intergenic
GCAAGTATCTTGGTTCTGAT+GGG 0.568688 1_2:+56724060 None:intergenic
ACTTCCTTAGCATTTGGAAG+GGG 0.569971 1_2:-56724647 Msa0079030:three_prime_UTR
TTTACTCACTATATGATGAA+AGG 0.573854 1_2:-56724749 Msa0079030:three_prime_UTR
CTAACCCCTTCCAAATGCTA+AGG 0.578712 1_2:+56724643 None:intergenic
ATAATTGTGGTATAGAAAAG+GGG 0.579060 1_2:-56724600 Msa0079030:three_prime_UTR
GGTGAAAAGCATGGTTTGAG+AGG 0.582986 1_2:-56723413 Msa0079030:three_prime_UTR
CATATAGATTCGAAATGGTG+TGG 0.585238 1_2:-56724402 Msa0079030:three_prime_UTR
AGATTTCACATTGCTCTGAG+TGG 0.588571 1_2:-56723953 Msa0079030:three_prime_UTR
GTCTGTGGAGCTCAGATCAA+GGG 0.592725 1_2:-56724937 Msa0079030:CDS
ATGTATATTCAACTATGTCA+TGG 0.593380 1_2:-56723166 Msa0079030:three_prime_UTR
TACAAAATAGGCTGGATCAG+TGG 0.595394 1_2:-56725148 Msa0079030:CDS
GATTTGTCAGATAATGGGGC+CGG 0.595817 1_2:-56726269 None:intergenic
TGCCTAGAAACTATAGTGCT+AGG 0.596157 1_2:-56724250 Msa0079030:three_prime_UTR
GTCGAAAGCATACAATGAAG+TGG 0.599287 1_2:-56723434 Msa0079030:three_prime_UTR
GGTTCTTGGATGTATAATTG+TGG 0.600693 1_2:-56724613 Msa0079030:three_prime_UTR
CATGTGTGTAACTGTGTCTG+TGG 0.600953 1_2:-56724952 Msa0079030:CDS
TGAGCATTGTTTGGGCTGAG+AGG 0.604872 1_2:-56724998 Msa0079030:CDS
CAATGAAGTGGTGAAAAGCA+TGG 0.608148 1_2:-56723422 Msa0079030:three_prime_UTR
GGTTCTGATGGGAATCTCAA+GGG 0.610471 1_2:+56724071 None:intergenic
GAAAGTGTTGCAAGACAGAT+GGG 0.611735 1_2:+56725481 None:intergenic
TACACACATGATGAGAGAAG+AGG 0.615179 1_2:+56724965 None:intergenic
ATGAAGATAGAAGAGATGGA+AGG 0.620527 1_2:+56724013 None:intergenic
TGAAAGTGTTGCAAGACAGA+TGG 0.621295 1_2:+56725480 None:intergenic
AAAGAAGTGAAAGTAGGTGA+AGG 0.622002 1_2:-56725173 Msa0079030:CDS
TAATTGTGGTATAGAAAAGG+GGG 0.630070 1_2:-56724599 Msa0079030:three_prime_UTR
ATTCAATGCATCATTAGACA+GGG 0.632087 1_2:-56724123 Msa0079030:three_prime_UTR
TAGAATCAATACAAGCCTGA+AGG 0.639426 1_2:-56725553 Msa0079030:CDS
GCTCAGATCAAGGGATATGG+AGG 0.647696 1_2:-56724928 Msa0079030:CDS
AATGATATGAATGCTAATGG+TGG 0.668855 1_2:-56725122 Msa0079030:CDS
AATGGTGTGGAACTGTATGT+GGG 0.689960 1_2:-56724389 Msa0079030:three_prime_UTR
TTCAATGCATCATTAGACAG+GGG 0.703105 1_2:-56724122 Msa0079030:three_prime_UTR
TTCATGAGTACAGTTGCTGG+TGG 0.729804 1_2:-56725260 Msa0079030:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAGTAAAGAAAATAA+AGG + chr1_2:56724571-56724590 None:intergenic 10.0%
!! TAATATTATATACTTTGATT+TGG - chr1_2:56724809-56724828 Msa0079030:three_prime_UTR 10.0%
!! TTTAATAGATAATAGATAAA+TGG + chr1_2:56725745-56725764 None:intergenic 10.0%
!!! TTTTTCTTTTTATATTTGTA+AGG - chr1_2:56726051-56726070 Msa0079030:intron 10.0%
!! AAACAGCAATAAAAAAATAA+TGG + chr1_2:56723489-56723508 None:intergenic 15.0%
!!! ATTTATTATTGTGATTAGTT+AGG - chr1_2:56724592-56724611 Msa0079030:three_prime_UTR 15.0%
!!! TTTATTATTGTGATTAGTTA+GGG - chr1_2:56724593-56724612 Msa0079030:three_prime_UTR 15.0%
!! AAATTTACCAAGAATTAATG+TGG + chr1_2:56723583-56723602 None:intergenic 20.0%
!! TAACAACTAAAACTGATATT+GGG + chr1_2:56723680-56723699 None:intergenic 20.0%
!! TTAACAACTAAAACTGATAT+TGG + chr1_2:56723681-56723700 None:intergenic 20.0%
!! TATAATTGTGGTATAGAAAA+GGG - chr1_2:56724780-56724799 Msa0079030:three_prime_UTR 20.0%
!!! TCAACATTGATATATTTTCT+TGG - chr1_2:56725166-56725185 Msa0079030:CDS 20.0%
!!! TAATAAAAGCATATAGTCAT+TGG + chr1_2:56725495-56725514 None:intergenic 20.0%
!!! AAATTCAATCTTTTACTTGT+TGG + chr1_2:56725579-56725598 None:intergenic 20.0%
!! AAGTAAAAGATTGAATTTGA+AGG - chr1_2:56725581-56725600 Msa0079030:intron 20.0%
!! GGTTTTCCTTTCATTTTATA+TGG - chr1_2:56723196-56723215 Msa0079030:three_prime_UTR 25.0%
! CAAAAGCCATATAAAATGAA+AGG + chr1_2:56723205-56723224 None:intergenic 25.0%
!!! TCATTTCCAGAATTTTGTTT+TGG - chr1_2:56723358-56723377 Msa0079030:three_prime_UTR 25.0%
!! TCTAATTTTTATGTTCTTGC+TGG - chr1_2:56723519-56723538 Msa0079030:intron 25.0%
! TTGACATATAAACTTGCAAA+GGG + chr1_2:56723631-56723650 None:intergenic 25.0%
! TTTGACATATAAACTTGCAA+AGG + chr1_2:56723632-56723651 None:intergenic 25.0%
!! TGTTAAACTACTTGATTTTG+AGG - chr1_2:56723695-56723714 Msa0079030:intron 25.0%
!! GTTAAACTACTTGATTTTGA+GGG - chr1_2:56723696-56723715 Msa0079030:intron 25.0%
!! TTAAACTACTTGATTTTGAG+GGG - chr1_2:56723697-56723716 Msa0079030:intron 25.0%
!! GTAGGAATAAAATGCATTTT+AGG - chr1_2:56723754-56723773 Msa0079030:intron 25.0%
!! TATTTGCCTGTTTACATTTT+AGG - chr1_2:56723804-56723823 Msa0079030:three_prime_UTR 25.0%
! TCATAATTACTGCAAGAAAA+AGG + chr1_2:56723937-56723956 None:intergenic 25.0%
!! TTTTCTTGCAGTAATTATGA+TGG - chr1_2:56723937-56723956 Msa0079030:three_prime_UTR 25.0%
! TTTCTTGCAGTAATTATGAT+GGG - chr1_2:56723938-56723957 Msa0079030:three_prime_UTR 25.0%
! TCTTATATTCTGTTACACTT+TGG - chr1_2:56724063-56724082 Msa0079030:three_prime_UTR 25.0%
! AGTAATGATATGAATGCTAA+TGG - chr1_2:56724256-56724275 Msa0079030:three_prime_UTR 25.0%
! CTTACTAAATCAATCAACTA+CGG + chr1_2:56724502-56724521 None:intergenic 25.0%
!! AGTTGATTGATTTAGTAAGT+TGG - chr1_2:56724503-56724522 Msa0079030:three_prime_UTR 25.0%
! TTTACTCACTATATGATGAA+AGG - chr1_2:56724632-56724651 Msa0079030:three_prime_UTR 25.0%
!! GATGAAAGGAAATTTAAAGT+TGG - chr1_2:56724646-56724665 Msa0079030:three_prime_UTR 25.0%
! TGTATTATCATTAGTTCCTA+AGG - chr1_2:56724683-56724702 Msa0079030:three_prime_UTR 25.0%
! GTATAATTGTGGTATAGAAA+AGG - chr1_2:56724779-56724798 Msa0079030:three_prime_UTR 25.0%
! ATAATTGTGGTATAGAAAAG+GGG - chr1_2:56724781-56724800 Msa0079030:three_prime_UTR 25.0%
! TTGGATGATTAATATGTATG+TGG - chr1_2:56724828-56724847 Msa0079030:three_prime_UTR 25.0%
! TGGATGATTAATATGTATGT+GGG - chr1_2:56724829-56724848 Msa0079030:three_prime_UTR 25.0%
! GGAGTATGAATATTAGAAAT+TGG + chr1_2:56725194-56725213 None:intergenic 25.0%
!! ATAGTGAATAACTTGCATTA+AGG + chr1_2:56725346-56725365 None:intergenic 25.0%
! GACAACACACATATATAAAA+AGG + chr1_2:56725456-56725475 None:intergenic 25.0%
! AATATACATGATAGCATAGT+AGG + chr1_2:56725721-56725740 None:intergenic 25.0%
!! TGTTCTCATTTTACATTAAC+TGG + chr1_2:56726163-56726182 None:intergenic 25.0%
! AATGTAAAATGAGAACATCT+TGG - chr1_2:56726166-56726185 Msa0079030:intron 25.0%
! GACATAGTTGAATATACATA+AGG + chr1_2:56726214-56726233 None:intergenic 25.0%
! ATGTATATTCAACTATGTCA+TGG - chr1_2:56726215-56726234 Msa0079030:intron 25.0%
!!! TTGCAGTTTTGTTTTGTTGT+TGG - chr1_2:56723239-56723258 Msa0079030:three_prime_UTR 30.0%
GGAACAATATCATGACAAAA+CGG + chr1_2:56723278-56723297 None:intergenic 30.0%
GGAGAAATCAATTGATATGT+TGG - chr1_2:56723403-56723422 Msa0079030:three_prime_UTR 30.0%
CTATCGTGAGGTAAAATTAT+TGG - chr1_2:56723603-56723622 Msa0079030:intron 30.0%
TTCTAGCCTAAAATGTAAAC+AGG + chr1_2:56723813-56723832 None:intergenic 30.0%
! TAAAAAGCTGGTCTACAATT+TGG - chr1_2:56723857-56723876 Msa0079030:three_prime_UTR 30.0%
! CAATTTGGAATATCCTATCA+AGG - chr1_2:56723872-56723891 Msa0079030:three_prime_UTR 30.0%
TTCTTGCAGTAATTATGATG+GGG - chr1_2:56723939-56723958 Msa0079030:three_prime_UTR 30.0%
GATTCAAAAGAAGTGAAAGT+AGG - chr1_2:56724202-56724221 Msa0079030:three_prime_UTR 30.0%
AATGATATGAATGCTAATGG+TGG - chr1_2:56724259-56724278 Msa0079030:three_prime_UTR 30.0%
!!! TGTTGCTTTTCAAAATCAGA+TGG - chr1_2:56724282-56724301 Msa0079030:three_prime_UTR 30.0%
TCAAAATCAGATGGAGAATA+TGG - chr1_2:56724291-56724310 Msa0079030:three_prime_UTR 30.0%
CAAAATCAGATGGAGAATAT+GGG - chr1_2:56724292-56724311 Msa0079030:three_prime_UTR 30.0%
CAGATTCTTCTTCTCTTTAT+TGG - chr1_2:56724320-56724339 Msa0079030:three_prime_UTR 30.0%
! ATTATTGTGATTAGTTAGGG+TGG - chr1_2:56724596-56724615 Msa0079030:three_prime_UTR 30.0%
TTGTTAACTTCCTTAGCATT+TGG - chr1_2:56724728-56724747 Msa0079030:three_prime_UTR 30.0%
TAATTGTGGTATAGAAAAGG+GGG - chr1_2:56724782-56724801 Msa0079030:three_prime_UTR 30.0%
GATTAATATGTATGTGGGAT+TGG - chr1_2:56724834-56724853 Msa0079030:three_prime_UTR 30.0%
TCAAAACTGTTACACACAAT+AGG - chr1_2:56725030-56725049 Msa0079030:CDS 30.0%
CAAAACTGTTACACACAATA+GGG - chr1_2:56725031-56725050 Msa0079030:CDS 30.0%
CAAACTTATATGCTGTCATT+TGG - chr1_2:56725071-56725090 Msa0079030:CDS 30.0%
AGTGAAATAAAAGATGCAAG+AGG + chr1_2:56725215-56725234 None:intergenic 30.0%
TATTCAATGCATCATTAGAC+AGG - chr1_2:56725257-56725276 Msa0079030:intron 30.0%
ATTCAATGCATCATTAGACA+GGG - chr1_2:56725258-56725277 Msa0079030:intron 30.0%
!!! GATTTTTTCTCCCTAACTTT+TGG - chr1_2:56725405-56725424 Msa0079030:intron 30.0%
TGTGAAATCTCCAAAAGTTA+GGG + chr1_2:56725418-56725437 None:intergenic 30.0%
ATGTGAAATCTCCAAAAGTT+AGG + chr1_2:56725419-56725438 None:intergenic 30.0%
AGATGACAGCTTTACTATTA+TGG - chr1_2:56725685-56725704 Msa0079030:intron 30.0%
ACAGCTTTACTATTATGGTA+TGG - chr1_2:56725690-56725709 Msa0079030:intron 30.0%
AAATTTGACAGCACAATAAG+AGG + chr1_2:56725779-56725798 None:intergenic 30.0%
! CACTAAAAGCATATAGTCAT+TGG + chr1_2:56725812-56725831 None:intergenic 30.0%
!!! CAGTTTTGTTTTGTTGTTGG+TGG - chr1_2:56723242-56723261 Msa0079030:three_prime_UTR 35.0%
!! GGTGCTGATGTTGTATTTAA+TGG - chr1_2:56723302-56723321 Msa0079030:three_prime_UTR 35.0%
TCACTGCCAAAACAAAATTC+TGG + chr1_2:56723367-56723386 None:intergenic 35.0%
!!! AGTGAGTTTTGCTATTGCAA+TGG - chr1_2:56723382-56723401 Msa0079030:three_prime_UTR 35.0%
ACTCATGGAGAATTAAGGAT+AGG - chr1_2:56723442-56723461 Msa0079030:three_prime_UTR 35.0%
CTCATGGAGAATTAAGGATA+GGG - chr1_2:56723443-56723462 Msa0079030:three_prime_UTR 35.0%
GCATCTACCACATTAATTCT+TGG - chr1_2:56723573-56723592 Msa0079030:intron 35.0%
CTTGGTAAATTTCTATCGTG+AGG - chr1_2:56723591-56723610 Msa0079030:intron 35.0%
TAGAATCAATACAAGCCTGA+AGG - chr1_2:56723828-56723847 Msa0079030:three_prime_UTR 35.0%
!! AGCTTTTTAATTGTGCCTTC+AGG + chr1_2:56723846-56723865 None:intergenic 35.0%
! TGAAGGCACAATTAAAAAGC+TGG - chr1_2:56723845-56723864 Msa0079030:three_prime_UTR 35.0%
TGCAAGAAAAAGGAGAAACA+TGG + chr1_2:56723927-56723946 None:intergenic 35.0%
CAAATTTACTCACTCACTCT+TGG - chr1_2:56723995-56724014 Msa0079030:three_prime_UTR 35.0%
CTCTTGGTTCACTATGTTAA+AGG - chr1_2:56724011-56724030 Msa0079030:three_prime_UTR 35.0%
AAATTCATGAGTACAGTTGC+TGG - chr1_2:56724118-56724137 Msa0079030:three_prime_UTR 35.0%
! TTGTGATCCTTTTCATCATG+AGG - chr1_2:56724168-56724187 Msa0079030:three_prime_UTR 35.0%
AAAGAAGTGAAAGTAGGTGA+AGG - chr1_2:56724208-56724227 Msa0079030:three_prime_UTR 35.0%
TAGGTGAAGGACTACAAAAT+AGG - chr1_2:56724221-56724240 Msa0079030:three_prime_UTR 35.0%
! TGATGTGTTGTTGTTGTTGT+TGG + chr1_2:56724356-56724375 None:intergenic 35.0%
AACAACAACAACACATCAAC+AGG - chr1_2:56724358-56724377 Msa0079030:three_prime_UTR 35.0%
! AACAGGTTTGAGCATTGTTT+GGG - chr1_2:56724375-56724394 Msa0079030:three_prime_UTR 35.0%
TTCCTAAGGAACTAATGTGT+TGG - chr1_2:56724697-56724716 Msa0079030:three_prime_UTR 35.0%
AACCAACACATTAGTTCCTT+AGG + chr1_2:56724702-56724721 None:intergenic 35.0%
TAACTTCCTTAGCATTTGGA+AGG - chr1_2:56724732-56724751 Msa0079030:three_prime_UTR 35.0%
AACTTCCTTAGCATTTGGAA+GGG - chr1_2:56724733-56724752 Msa0079030:three_prime_UTR 35.0%
GGTTCTTGGATGTATAATTG+TGG - chr1_2:56724768-56724787 Msa0079030:three_prime_UTR 35.0%
!! ATATGTATGTGGGATTGGTT+TGG - chr1_2:56724839-56724858 Msa0079030:three_prime_UTR 35.0%
CCATTTCGAATCTATATGCT+TGG + chr1_2:56724977-56724996 None:intergenic 35.0%
CCAAGCATATAGATTCGAAA+TGG - chr1_2:56724974-56724993 Msa0079030:CDS 35.0%
CATATAGATTCGAAATGGTG+TGG - chr1_2:56724979-56724998 Msa0079030:CDS 35.0%
! ACTTATATGCTGTCATTTGG+TGG - chr1_2:56725074-56725093 Msa0079030:CDS 35.0%
TTCAATGCATCATTAGACAG+GGG - chr1_2:56725259-56725278 Msa0079030:intron 35.0%
TTGCATTAAGGCAAGTATCT+TGG + chr1_2:56725334-56725353 None:intergenic 35.0%
ATGAAGATAGAAGAGATGGA+AGG + chr1_2:56725371-56725390 None:intergenic 35.0%
TGACATGAAGATAGAAGAGA+TGG + chr1_2:56725375-56725394 None:intergenic 35.0%
TGTGTTGTCAACTTTGTTCA+TGG - chr1_2:56725467-56725486 Msa0079030:CDS 35.0%
CGATTTACTTTGTGATCACT+TGG - chr1_2:56725523-56725542 Msa0079030:CDS 35.0%
!! TTTTACTTGTTGGAGCTTCA+TGG + chr1_2:56725569-56725588 None:intergenic 35.0%
GTGTTGTCCTTATGCTTAAA+AGG - chr1_2:56725621-56725640 Msa0079030:intron 35.0%
TTGTCCTTATGCTTAAAAGG+TGG - chr1_2:56725624-56725643 Msa0079030:intron 35.0%
CTGTCATCTAGAAAGGAAAA+AGG + chr1_2:56725674-56725693 None:intergenic 35.0%
AGTAAAGCTGTCATCTAGAA+AGG + chr1_2:56725681-56725700 None:intergenic 35.0%
TGTGCTGTCAAATTTGATCA+TGG - chr1_2:56725784-56725803 Msa0079030:intron 35.0%
! CTATATGCTTTTAGTGCTGT+CGG - chr1_2:56725816-56725835 Msa0079030:intron 35.0%
! TCATTTTGCTGATGAGATGT+GGG - chr1_2:56725886-56725905 Msa0079030:intron 35.0%
!!! ATTTGGTGTCTTTTTTGTGC+AGG - chr1_2:56725911-56725930 Msa0079030:intron 35.0%
!!! TTTGGTGTCTTTTTTGTGCA+GGG - chr1_2:56725912-56725931 Msa0079030:intron 35.0%
GCCGGTACTTGAAAAATTTC+CGG - chr1_2:56723130-56723149 Msa0079030:three_prime_UTR 40.0%
!!! GCCGGAAATTTTTCAAGTAC+CGG + chr1_2:56723134-56723153 None:intergenic 40.0%
GGTACTTGAAAAATTTCCGG+CGG - chr1_2:56723133-56723152 Msa0079030:three_prime_UTR 40.0%
TCAGGTCCATAATTTCTCTG+TGG - chr1_2:56723165-56723184 Msa0079030:three_prime_UTR 40.0%
GTCCATAATTTCTCTGTGGT+GGG - chr1_2:56723169-56723188 Msa0079030:three_prime_UTR 40.0%
TCCATAATTTCTCTGTGGTG+GGG - chr1_2:56723170-56723189 Msa0079030:three_prime_UTR 40.0%
ATCATGACAAAACGGCAATC+AGG + chr1_2:56723270-56723289 None:intergenic 40.0%
TGTCATGATATTGTTCCGTG+TGG - chr1_2:56723281-56723300 Msa0079030:three_prime_UTR 40.0%
AATACAACATCAGCACCACA+CGG + chr1_2:56723299-56723318 None:intergenic 40.0%
! ATGTTGGCTGACATTGTTCA+TGG - chr1_2:56723419-56723438 Msa0079030:three_prime_UTR 40.0%
TGACATTGTTCATGGACTCA+TGG - chr1_2:56723427-56723446 Msa0079030:three_prime_UTR 40.0%
CATGGACTCATGGAGAATTA+AGG - chr1_2:56723437-56723456 Msa0079030:three_prime_UTR 40.0%
!! AACTACTTGATTTTGAGGGG+TGG - chr1_2:56723700-56723719 Msa0079030:intron 40.0%
!! ACTACTTGATTTTGAGGGGT+GGG - chr1_2:56723701-56723720 Msa0079030:intron 40.0%
GTAGGGCTAGAATCAATAGT+AGG - chr1_2:56723736-56723755 Msa0079030:intron 40.0%
!! GAAAGTGTTGCAAGACAGAT+GGG + chr1_2:56723903-56723922 None:intergenic 40.0%
!! TGAAAGTGTTGCAAGACAGA+TGG + chr1_2:56723904-56723923 None:intergenic 40.0%
! TGCAGTAATTATGATGGGGT+TGG - chr1_2:56723943-56723962 Msa0079030:three_prime_UTR 40.0%
GTTGCAGTAGTGTAGAGTTA+AGG + chr1_2:56724096-56724115 None:intergenic 40.0%
ATTCCAGCCTCATGATGAAA+AGG + chr1_2:56724178-56724197 None:intergenic 40.0%
TGAAGGACTACAAAATAGGC+TGG - chr1_2:56724225-56724244 Msa0079030:three_prime_UTR 40.0%
TACAAAATAGGCTGGATCAG+TGG - chr1_2:56724233-56724252 Msa0079030:three_prime_UTR 40.0%
! CAACAGGTTTGAGCATTGTT+TGG - chr1_2:56724374-56724393 Msa0079030:three_prime_UTR 40.0%
TGATGAGAGAAGAGGATTTG+AGG + chr1_2:56724411-56724430 None:intergenic 40.0%
TACACACATGATGAGAGAAG+AGG + chr1_2:56724419-56724438 None:intergenic 40.0%
GGATATGGAGGTTCTTCATT+TGG - chr1_2:56724465-56724484 Msa0079030:three_prime_UTR 40.0%
ACTTCCTTAGCATTTGGAAG+GGG - chr1_2:56724734-56724753 Msa0079030:three_prime_UTR 40.0%
ATTTGGAAGGGGTTAGGTTA+TGG - chr1_2:56724745-56724764 Msa0079030:three_prime_UTR 40.0%
TTTGGAAGGGGTTAGGTTAT+GGG - chr1_2:56724746-56724765 Msa0079030:three_prime_UTR 40.0%
!! TGTGGGATTGGTTTGGTATT+TGG - chr1_2:56724846-56724865 Msa0079030:three_prime_UTR 40.0%
!! TTGGTTTGGTATTTGGTGTG+TGG - chr1_2:56724853-56724872 Msa0079030:three_prime_UTR 40.0%
AAATGGTGTGGAACTGTATG+TGG - chr1_2:56724991-56725010 Msa0079030:CDS 40.0%
AATGGTGTGGAACTGTATGT+GGG - chr1_2:56724992-56725011 Msa0079030:CDS 40.0%
TGCCTAGAAACTATAGTGCT+AGG - chr1_2:56725131-56725150 Msa0079030:CDS 40.0%
CACCTAGCACTATAGTTTCT+AGG + chr1_2:56725136-56725155 None:intergenic 40.0%
AAACTATAGTGCTAGGTGAC+AGG - chr1_2:56725138-56725157 Msa0079030:CDS 40.0%
!! GCAAGTATCTTGGTTCTGAT+GGG + chr1_2:56725324-56725343 None:intergenic 40.0%
! AGATTTCACATTGCTCTGAG+TGG - chr1_2:56725428-56725447 Msa0079030:intron 40.0%
GCATAAGGACAACACTTACA+TGG + chr1_2:56725616-56725635 None:intergenic 40.0%
! ACAGCCACCTTTTAAGCATA+AGG + chr1_2:56725631-56725650 None:intergenic 40.0%
ACAGCACAATAAGAGGTTTC+AGG + chr1_2:56725772-56725791 None:intergenic 40.0%
!! GTCATTTTGCTGATGAGATG+TGG - chr1_2:56725885-56725904 Msa0079030:intron 40.0%
! CTGATGAGATGTGGGTTATT+TGG - chr1_2:56725894-56725913 Msa0079030:intron 40.0%
! GTCGAAAGCATACAATGAAG+TGG - chr1_2:56725947-56725966 Msa0079030:intron 40.0%
CAATGAAGTGGTGAAAAGCA+TGG - chr1_2:56725959-56725978 Msa0079030:intron 40.0%
GGTCCATAATTTCTCTGTGG+TGG - chr1_2:56723168-56723187 Msa0079030:three_prime_UTR 45.0%
ACCCCACCACAGAGAAATTA+TGG + chr1_2:56723174-56723193 None:intergenic 45.0%
! TAATTTCTCTGTGGTGGGGT+TGG - chr1_2:56723174-56723193 Msa0079030:three_prime_UTR 45.0%
! AATTTCTCTGTGGTGGGGTT+GGG - chr1_2:56723175-56723194 Msa0079030:three_prime_UTR 45.0%
!! TTGATTTTGAGGGGTGGGTT+GGG - chr1_2:56723706-56723725 Msa0079030:intron 45.0%
!! GTGGGTTGGGTAATGTAGTA+GGG - chr1_2:56723719-56723738 Msa0079030:intron 45.0%
CAGATGGGACAAACCTTGAT+AGG + chr1_2:56723888-56723907 None:intergenic 45.0%
TTCATGAGTACAGTTGCTGG+TGG - chr1_2:56724121-56724140 Msa0079030:three_prime_UTR 45.0%
! GATCCTTTTCATCATGAGGC+TGG - chr1_2:56724172-56724191 Msa0079030:three_prime_UTR 45.0%
TCATCATGAGGCTGGAATGA+TGG - chr1_2:56724180-56724199 Msa0079030:three_prime_UTR 45.0%
CATGTGTGTAACTGTGTCTG+TGG - chr1_2:56724429-56724448 Msa0079030:three_prime_UTR 45.0%
! TGTGATTAGTTAGGGTGGTG+AGG - chr1_2:56724601-56724620 Msa0079030:three_prime_UTR 45.0%
ATTAGTTAGGGTGGTGAGGT+TGG - chr1_2:56724605-56724624 Msa0079030:three_prime_UTR 45.0%
CTAACCCCTTCCAAATGCTA+AGG + chr1_2:56724741-56724760 None:intergenic 45.0%
CTTAGCATTTGGAAGGGGTT+AGG - chr1_2:56724739-56724758 Msa0079030:three_prime_UTR 45.0%
TTGGAAGGGGTTAGGTTATG+GGG - chr1_2:56724747-56724766 Msa0079030:three_prime_UTR 45.0%
! ATATGCTGTCATTTGGTGGC+AGG - chr1_2:56725078-56725097 Msa0079030:CDS 45.0%
! TATGCTGTCATTTGGTGGCA+GGG - chr1_2:56725079-56725098 Msa0079030:CDS 45.0%
GCATCATTAGACAGGGGTAA+AGG - chr1_2:56725265-56725284 Msa0079030:intron 45.0%
! GGTTCTGATGGGAATCTCAA+GGG + chr1_2:56725313-56725332 None:intergenic 45.0%
!! TGGTTCTGATGGGAATCTCA+AGG + chr1_2:56725314-56725333 None:intergenic 45.0%
!! GGCAAGTATCTTGGTTCTGA+TGG + chr1_2:56725325-56725344 None:intergenic 45.0%
! GGTGAAAAGCATGGTTTGAG+AGG - chr1_2:56725968-56725987 Msa0079030:intron 45.0%
TTCCGGCGGTACTTCAATTC+AGG - chr1_2:56723147-56723166 Msa0079030:three_prime_UTR 50.0%
!! GACCTGAATTGAAGTACCGC+CGG + chr1_2:56723152-56723171 None:intergenic 50.0%
!! CTTGATTTTGAGGGGTGGGT+TGG - chr1_2:56723705-56723724 Msa0079030:intron 50.0%
!! GGTGGGTTGGGTAATGTAGT+AGG - chr1_2:56723718-56723737 Msa0079030:intron 50.0%
ATGAGTACAGTTGCTGGTGG+TGG - chr1_2:56724124-56724143 Msa0079030:three_prime_UTR 50.0%
! TGAGCATTGTTTGGGCTGAG+AGG - chr1_2:56724383-56724402 Msa0079030:three_prime_UTR 50.0%
TGTCTGTGGAGCTCAGATCA+AGG - chr1_2:56724443-56724462 Msa0079030:three_prime_UTR 50.0%
GTCTGTGGAGCTCAGATCAA+GGG - chr1_2:56724444-56724463 Msa0079030:three_prime_UTR 50.0%
GGAGCTCAGATCAAGGGATA+TGG - chr1_2:56724450-56724469 Msa0079030:three_prime_UTR 50.0%
GCTCAGATCAAGGGATATGG+AGG - chr1_2:56724453-56724472 Msa0079030:three_prime_UTR 50.0%
GGGTTAGGTTATGGGGTTCT+TGG - chr1_2:56724754-56724773 Msa0079030:three_prime_UTR 50.0%
! GTGGAACTGTATGTGGGCTA+TGG - chr1_2:56724998-56725017 Msa0079030:CDS 50.0%
! GTATGTGGGCTATGGTAGTC+TGG - chr1_2:56725006-56725025 Msa0079030:CDS 50.0%
!! AGTACAGTTGCTGGTGGTGG+TGG - chr1_2:56724127-56724146 Msa0079030:three_prime_UTR 55.0%
!! GGTGGTGGTGGTGGAATGAA+AGG - chr1_2:56724145-56724164 Msa0079030:three_prime_UTR 55.0%
AGTTAGGGTGGTGAGGTTGG+TGG - chr1_2:56724608-56724627 Msa0079030:three_prime_UTR 55.0%
!! ACAGTTGCTGGTGGTGGTGG+TGG - chr1_2:56724130-56724149 Msa0079030:three_prime_UTR 60.0%
!! GTTGCTGGTGGTGGTGGTGG+TGG - chr1_2:56724133-56724152 Msa0079030:three_prime_UTR 65.0%
!! GCTGGTGGTGGTGGTGGTGG+TGG - chr1_2:56724136-56724155 Msa0079030:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 56723125 56726278 56723125 ID=Msa0079030;Name=Msa0079030
chr1_2 mRNA 56723125 56726278 56723125 ID=Msa0079030-mRNA-1;Parent=Msa0079030;Name=Msa0079030-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0.33|0.5|1|0|0.25|4|1458|166
chr1_2 exon 56723125 56723470 56723125 ID=Msa0079030-mRNA-1:exon:17849;Parent=Msa0079030-mRNA-1
chr1_2 exon 56723787 56725269 56723787 ID=Msa0079030-mRNA-1:exon:17848;Parent=Msa0079030-mRNA-1
chr1_2 exon 56725457 56725577 56725457 ID=Msa0079030-mRNA-1:exon:17847;Parent=Msa0079030-mRNA-1
chr1_2 exon 56726270 56726278 56726270 ID=Msa0079030-mRNA-1:exon:17846;Parent=Msa0079030-mRNA-1
chr1_2 CDS 56726270 56726278 56726270 ID=Msa0079030-mRNA-1:cds;Parent=Msa0079030-mRNA-1
chr1_2 CDS 56725457 56725577 56725457 ID=Msa0079030-mRNA-1:cds;Parent=Msa0079030-mRNA-1
chr1_2 CDS 56724899 56725269 56724899 ID=Msa0079030-mRNA-1:cds;Parent=Msa0079030-mRNA-1
chr1_2 three_prime_UTR 56723787 56724898 56723787 ID=Msa0079030-mRNA-1:three_prime_utr;Parent=Msa0079030-mRNA-1
chr1_2 three_prime_UTR 56723125 56723470 56723125 ID=Msa0079030-mRNA-1:three_prime_utr;Parent=Msa0079030-mRNA-1
Gene Sequence

>Msa0079030

ATGGGGCCGGCTAGAATCAATACAAGCCTGAAGGCACAATTAAAAAGCTGGTCTACAATTTGGAATATCCTATCAAGGTTTGTCCCATCTGTCTTGCAACACTTTCACCATGTTTCTCCTTTTTCTTGCATTGCTGGTGGTGGTGGTGGTGGTGGAATGAAAGGTTGTGATCCTTTTCATCATGAGGCTGGAATGATGGATTCAAAAGAAGTGAAAGTAGGTGAAGGACTACAAAATAGGCTGGATCAGTGGAGTAATGATATGAATGCTAATGGTGGTGTTGCTTTTCAAAATCAGATGGAGAATATGGGTTTGTCAGATTCTTCTTCTCTTTATTGGAATAACAACACCAACAACAACAACAACACATCAACAGGTTTGAGCATTGTTTGGGCTGAGAGGAACCTCAAATCCTCTTCTCTCATCATGTGTGTAACTGTGTCTGTGGAGCTCAGATCAAGGGATATGGAGGTTCTTCATTTGGTGCAAGTACTTCCGTAG
Protein sequence

>Msa0079030

MGPARINTSLKAQLKSWSTIWNILSRFVPSVLQHFHHVSPFSCIAGGGGGGGMKGCDPFHHEAGMMDSKEVKVGEGLQNRLDQWSNDMNANGGVAFQNQMENMGLSDSSSLYWNNNTNNNNNTSTGLSIVWAERNLKSSSLIMCVTVSVELRSRDMEVLHLVQVLP*