AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0082040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0082040 MtrunA17_Chr1g0194891 98.558 208 3 0 1 208 1 208 7.54e-152 419
Msa0082040 MtrunA17_Chr4g0014721 29.268 123 87 0 32 154 228 350 4.45e-15 73.2
Msa0082040 MtrunA17_Chr4g0014741 29.268 123 87 0 32 154 228 350 4.50e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0082040 AT3G62580.1 74.648 213 49 1 1 208 1 213 1.20e-115 328
Msa0082040 AT1G72100.1 28.723 188 121 4 25 206 296 476 9.96e-14 69.7
Msa0082040 AT1G22600.1 28.342 187 114 4 41 208 198 383 1.01e-11 63.5

Find 85 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGCTTTCGGCGCCGCTCTA+TGG 0.164177 1_2:+60837437 Msa0082040:CDS
TTATTCATCTTTGCAAGCTT+TGG 0.242253 1_2:-60840041 None:intergenic
GATTCCAGCGCTCGTGGGTT+TGG 0.328551 1_2:-60837148 None:intergenic
CAATATGGGGCAGCTGTATT+AGG 0.336814 1_2:+60837082 None:intergenic
CTTGCCAACATCATGTCATT+TGG 0.339373 1_2:+60840095 Msa0082040:CDS
CGCTTTCGGCGCCGCTCTAT+GGG 0.344664 1_2:+60837438 Msa0082040:CDS
AATAGTATTGGAGAGGAAGT+TGG 0.347347 1_2:+60839984 Msa0082040:CDS
AAGAAGTTTGGTATGATTCA+TGG 0.351606 1_2:+60840062 Msa0082040:CDS
CTCGGCTTCTCCATCGCTTT+CGG 0.353954 1_2:+60837424 Msa0082040:CDS
AATTTGCCTAGGCATCAATT+TGG 0.358606 1_2:+60837594 Msa0082040:CDS
GCTGTCACTGTGGCTTCCTT+TGG 0.364294 1_2:+60837672 Msa0082040:CDS
TTTCTCAGTGTGCTATTCTC+TGG 0.370714 1_2:+60840414 Msa0082040:three_prime_UTR
TGACGGTGGTGGTGGTGTTC+TGG 0.374589 1_2:-60837275 None:intergenic
AACCCACGAGCGCTGGAATC+TGG 0.377154 1_2:+60837151 Msa0082040:five_prime_UTR
GCAAAGATGAATAAGAAGTT+TGG 0.383264 1_2:+60840050 Msa0082040:CDS
ATTATGCTATAAATTATATA+AGG 0.396138 1_2:+60840378 Msa0082040:three_prime_UTR
TGCAATACACTCTTGGTACT+TGG 0.405780 1_2:+60840124 Msa0082040:CDS
TGGTGTAAAGACGAACAAAT+TGG 0.408776 1_2:-60837778 None:intergenic
TCTAGAAACCGAAGATCATT+TGG 0.420817 1_2:+60837120 Msa0082040:five_prime_UTR
ATAGTATTGGAGAGGAAGTT+GGG 0.421614 1_2:+60839985 Msa0082040:CDS
CGAGGAGATGAGCGAGTTTG+AGG 0.436447 1_2:-60837405 None:intergenic
TGGATTGACCAAATGATCTT+CGG 0.439017 1_2:-60837128 None:intergenic
GACAAGTATCTCTACTAGAA+TGG 0.440265 1_2:-60840182 None:intergenic
GTGGAGAGAGAGAATAGTAT+TGG 0.444526 1_2:+60839972 Msa0082040:CDS
TAATGAGGTAGTTGAATTGA+AGG 0.451077 1_2:-60837238 None:intergenic
GAGATACTTGTCTGTGCAAT+TGG 0.459576 1_2:+60840193 Msa0082040:three_prime_UTR
CAATCCAAACCCACGAGCGC+TGG 0.468537 1_2:+60837144 Msa0082040:five_prime_UTR
ACAAGTATCTCTACTAGAAT+GGG 0.473721 1_2:-60840181 None:intergenic
AGAAGTTTGGTATGATTCAT+GGG 0.477765 1_2:+60840063 Msa0082040:CDS
GAAAATACCTCAATTGTCAT+TGG 0.481213 1_2:-60837798 None:intergenic
ATGAGCGAGTTTGAGGTAAG+TGG 0.481460 1_2:-60837398 None:intergenic
GAAGCCTTGCAATACACTCT+TGG 0.483767 1_2:+60840117 Msa0082040:CDS
GCTCTATGGGTCACCTTCAT+CGG 0.492086 1_2:+60837451 Msa0082040:CDS
ACGAGCGCTGGAATCTGGTT+TGG 0.492118 1_2:+60837156 Msa0082040:five_prime_UTR
ATTGATTTACCAAGAGTATC+CGG 0.495468 1_2:+60840245 Msa0082040:three_prime_UTR
ACCACCACCACCGTCATCAA+TGG 0.497424 1_2:+60837282 Msa0082040:exon
AGATTACCAAATTGATGCCT+AGG 0.499514 1_2:-60837600 None:intergenic
TGGTGTTCTGGTTCTGAGAA+AGG 0.511223 1_2:-60837263 None:intergenic
AATGAGGTAGTTGAATTGAA+GGG 0.512893 1_2:-60837237 None:intergenic
CCACCACCACCGTCATCAAT+GGG 0.515355 1_2:+60837283 Msa0082040:exon
CTCAAACTCGCTCATCTCCT+CGG 0.520112 1_2:+60837406 Msa0082040:CDS
GATGAAACAAAGGCATAAAG+TGG 0.520254 1_2:+60839953 Msa0082040:CDS
CCCATTGATGACGGTGGTGG+TGG 0.521011 1_2:-60837283 None:intergenic
CTTTACACCAATGACAATTG+AGG 0.521289 1_2:+60837791 Msa0082040:CDS
ATACACTCTTGGTACTTGGC+AGG 0.533564 1_2:+60840128 Msa0082040:CDS
AGCAAGACACCGGATACTCT+TGG 0.534506 1_2:-60840254 None:intergenic
GAGAGAGAATAGTATTGGAG+AGG 0.541841 1_2:+60839977 Msa0082040:CDS
TTGTGTTTAGGAATTTGCCT+AGG 0.542157 1_2:+60837583 Msa0082040:intron
TGGTGAGAAAATTACTCCGA+TGG 0.542306 1_2:-60837344 None:intergenic
TTAGCATGTGGGGAATAAAA+CGG 0.543297 1_2:+60840468 Msa0082040:three_prime_UTR
AAACCAGATTCCAGCGCTCG+TGG 0.544546 1_2:-60837154 None:intergenic
TAGAGCGGCGCCGAAAGCGA+TGG 0.548120 1_2:-60837434 None:intergenic
CAAATGACATGATGTTGGCA+AGG 0.567231 1_2:-60840094 None:intergenic
CATGATGATACCGCCGATGA+AGG 0.570088 1_2:-60837464 None:intergenic
AAGTTGGTATCTTTCACTAG+TGG 0.574244 1_2:-60837721 None:intergenic
CGAAAGCGATGGAGAAGCCG+AGG 0.581248 1_2:-60837423 None:intergenic
ATGAGGTAGTTGAATTGAAG+GGG 0.581274 1_2:-60837236 None:intergenic
TTTGAGGTAAGTGGAAAGAG+TGG 0.582513 1_2:-60837389 None:intergenic
ATGTACCTTTCTGTAGTCAA+AGG 0.582953 1_2:+60840346 Msa0082040:three_prime_UTR
GAGGTAAGTGGAAAGAGTGG+TGG 0.583033 1_2:-60837386 None:intergenic
TTTGACTACAGAAAGGTACA+TGG 0.584102 1_2:-60840344 None:intergenic
ACTAGTGAAAGATACCAACT+TGG 0.589922 1_2:+60837723 Msa0082040:CDS
CCTTTGGTTATCTTCATCCG+TGG 0.593206 1_2:+60837688 Msa0082040:CDS
GCTTCCAAATGACATGATGT+TGG 0.594501 1_2:-60840099 None:intergenic
CAGAGATCAAGAAACCAAGT+TGG 0.596357 1_2:-60837737 None:intergenic
AAATGACATGATGTTGGCAA+GGG 0.609102 1_2:-60840093 None:intergenic
AACCAGATTCCAGCGCTCGT+GGG 0.615154 1_2:-60837153 None:intergenic
TGATATAGATGATGAAACAA+AGG 0.622671 1_2:+60839943 Msa0082040:intron
AGTATCTCTACTAGAATGGG+AGG 0.623701 1_2:-60840178 None:intergenic
CCACGGATGAAGATAACCAA+AGG 0.626168 1_2:-60837688 None:intergenic
ATGTTTGCACAGCAAGACAC+CGG 0.626366 1_2:-60840264 None:intergenic
CTACCCATTGATGACGGTGG+TGG 0.630995 1_2:-60837286 None:intergenic
GCGGCTCTACCCATTGATGA+CGG 0.643113 1_2:-60837292 None:intergenic
CTATGGGTCACCTTCATCGG+CGG 0.646660 1_2:+60837454 Msa0082040:CDS
GTATACCTTTGACTACAGAA+AGG 0.656498 1_2:-60840351 None:intergenic
AACCGCGGTTGTTATTAGAG+CGG 0.674556 1_2:-60837311 None:intergenic
GATGAAGGTGACCCATAGAG+CGG 0.683702 1_2:-60837449 None:intergenic
GTTCTGAGAAAGGAGTAATG+AGG 0.684686 1_2:-60837253 None:intergenic
AGTACCAAGAGTGTATTGCA+AGG 0.685013 1_2:-60840121 None:intergenic
TGTTTGCATTGCTGTCACTG+TGG 0.686509 1_2:+60837662 Msa0082040:CDS
TGTACTATCAAAGCATCACA+CGG 0.689750 1_2:+60840221 Msa0082040:three_prime_UTR
GCTCTACCCATTGATGACGG+TGG 0.693373 1_2:-60837289 None:intergenic
GTATTGGAGAGGAAGTTGGG+TGG 0.710494 1_2:+60839988 Msa0082040:CDS
AGCCGCTCTAATAACAACCG+CGG 0.732310 1_2:+60837309 Msa0082040:CDS
GATGGCTAAGAAAACAACCG+CGG 0.788667 1_2:-60837326 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATTAAATTAACATAATTA+GGG - chr1_2:60837535-60837554 None:intergenic 10.0%
!! ATTATGCTATAAATTATATA+AGG + chr1_2:60840378-60840397 Msa0082040:three_prime_UTR 10.0%
!! GATTAAATTAACATAATTAG+GGG - chr1_2:60837534-60837553 None:intergenic 15.0%
!! CTGATTAAATTAACATAATT+AGG - chr1_2:60837536-60837555 None:intergenic 15.0%
!!! TATTTTATTCAAGTAAACTA+AGG - chr1_2:60840318-60840337 None:intergenic 15.0%
!!! TTCTTTTCTTTGTCTTTATA+AGG + chr1_2:60837840-60837859 Msa0082040:intron 20.0%
!!! ATGTTTATAGAATTTTGACA+TGG - chr1_2:60838404-60838423 None:intergenic 20.0%
!! AAATGAAAGAATCAAGAAAA+GGG - chr1_2:60838607-60838626 None:intergenic 20.0%
!! AAAATGAAAGAATCAAGAAA+AGG - chr1_2:60838608-60838627 None:intergenic 20.0%
!! ATTACTTTACCAAATATGTA+TGG + chr1_2:60838649-60838668 Msa0082040:intron 20.0%
!! AATATTTCACCATACATATT+TGG - chr1_2:60838661-60838680 None:intergenic 20.0%
!! AATTTGCCTTTAAATTTAAG+CGG - chr1_2:60839100-60839119 None:intergenic 20.0%
!! TGTAAAAGATGGTAAAAAAT+AGG + chr1_2:60839306-60839325 Msa0082040:intron 20.0%
!! AACTATTAGAAAAGATTTAG+AGG + chr1_2:60839448-60839467 Msa0082040:intron 20.0%
!! AATATAGCATAACATGTATA+GGG - chr1_2:60839619-60839638 None:intergenic 20.0%
!! TAATATAGCATAACATGTAT+AGG - chr1_2:60839620-60839639 None:intergenic 20.0%
!! TTGTAACATGATTTATGTTA+GGG - chr1_2:60839734-60839753 None:intergenic 20.0%
!! TCTAATAAAAAATTAGCATG+TGG + chr1_2:60840456-60840475 Msa0082040:three_prime_UTR 20.0%
!! CTAATAAAAAATTAGCATGT+GGG + chr1_2:60840457-60840476 Msa0082040:three_prime_UTR 20.0%
!! TAATAAAAAATTAGCATGTG+GGG + chr1_2:60840458-60840477 Msa0082040:three_prime_UTR 20.0%
! GAAATTGATTAATTGATGGA+TGG - chr1_2:60837512-60837531 None:intergenic 25.0%
! AGAAGAATAATGAGAAAAGA+CGG + chr1_2:60837941-60837960 Msa0082040:intron 25.0%
!!! ACCTATGCATAAGTTTTATT+AGG + chr1_2:60838059-60838078 Msa0082040:intron 25.0%
!!! CCTATGCATAAGTTTTATTA+GGG + chr1_2:60838060-60838079 Msa0082040:intron 25.0%
! GATTATAGATATCTCTACAA+GGG - chr1_2:60838280-60838299 None:intergenic 25.0%
!! CTTGTATTGCATAAGTTTTT+AGG + chr1_2:60838726-60838745 Msa0082040:intron 25.0%
! AAAAAATATACCGATGGTTA+AGG - chr1_2:60838763-60838782 None:intergenic 25.0%
! CAATTCAAAAAATATACCGA+TGG - chr1_2:60838769-60838788 None:intergenic 25.0%
! AACAATAACAACAAATGTTC+AGG + chr1_2:60838912-60838931 Msa0082040:intron 25.0%
! CAAAAAACAAATCGAACAAA+AGG - chr1_2:60838972-60838991 None:intergenic 25.0%
!!! CTTTTGTTCGATTTGTTTTT+TGG + chr1_2:60838970-60838989 Msa0082040:intron 25.0%
! TACTTTCATATTGCATGTAT+AGG - chr1_2:60839077-60839096 None:intergenic 25.0%
!!! AAAGTACCGCTTAAATTTAA+AGG + chr1_2:60839091-60839110 Msa0082040:intron 25.0%
!! TTTACCATCTTTTACACTAT+AGG - chr1_2:60839302-60839321 None:intergenic 25.0%
! TACAATACTCATAACATTGT+CGG + chr1_2:60839643-60839662 Msa0082040:intron 25.0%
! GTTGTAACATGATTTATGTT+AGG - chr1_2:60839735-60839754 None:intergenic 25.0%
! TAAAGTTTGAAAACTTACGT+TGG - chr1_2:60839803-60839822 None:intergenic 25.0%
! TCTATATCAGACAAACAAAA+TGG - chr1_2:60839932-60839951 None:intergenic 25.0%
! TGATATAGATGATGAAACAA+AGG + chr1_2:60839943-60839962 Msa0082040:intron 25.0%
AATGAGGTAGTTGAATTGAA+GGG - chr1_2:60837240-60837259 None:intergenic 30.0%
TAATGAGGTAGTTGAATTGA+AGG - chr1_2:60837241-60837260 None:intergenic 30.0%
! ATTTTCTCACCAGAAACTTT+CGG + chr1_2:60837355-60837374 Msa0082040:CDS 30.0%
AGGGGAAATTGATTAATTGA+TGG - chr1_2:60837516-60837535 None:intergenic 30.0%
!! GTTTCCAGCTTATTTTTCAA+TGG + chr1_2:60837635-60837654 Msa0082040:CDS 30.0%
GAAAATACCTCAATTGTCAT+TGG - chr1_2:60837801-60837820 None:intergenic 30.0%
GAAGAATAATGAGAAAAGAC+GGG + chr1_2:60837942-60837961 Msa0082040:intron 30.0%
GTAAGTTATTCATCCAAACA+TGG - chr1_2:60838033-60838052 None:intergenic 30.0%
CCCTAATAAAACTTATGCAT+AGG - chr1_2:60838063-60838082 None:intergenic 30.0%
CGATTATAGATATCTCTACA+AGG - chr1_2:60838281-60838300 None:intergenic 30.0%
!!! GATAAGCTTTTTCAAACACT+AGG + chr1_2:60838300-60838319 Msa0082040:intron 30.0%
! GTAAGCTCAAATAAGCTTTT+TGG + chr1_2:60838338-60838357 Msa0082040:intron 30.0%
TCTTGCAATTGAATCATCTA+AGG + chr1_2:60838435-60838454 Msa0082040:intron 30.0%
CTAACAGGAATCAACATTTA+AGG - chr1_2:60838466-60838485 None:intergenic 30.0%
TGATTCCTGTTAGTAATTAG+AGG + chr1_2:60838473-60838492 Msa0082040:intron 30.0%
GATTCCTGTTAGTAATTAGA+GGG + chr1_2:60838474-60838493 Msa0082040:intron 30.0%
! CTAAATGTTCTCTTTTGTGT+TGG + chr1_2:60838529-60838548 Msa0082040:intron 30.0%
! GTAATGTTTTCCTTAACCAT+CGG + chr1_2:60838750-60838769 Msa0082040:intron 30.0%
ATTTCCTGTTCTATCTTCTT+TGG + chr1_2:60838840-60838859 Msa0082040:intron 30.0%
AAATCAGTCAACACAAAAGT+AGG + chr1_2:60839002-60839021 Msa0082040:intron 30.0%
AATCAGTCAACACAAAAGTA+GGG + chr1_2:60839003-60839022 Msa0082040:intron 30.0%
TATTGCATGTATAGGCAAAA+TGG - chr1_2:60839069-60839088 None:intergenic 30.0%
AAGTAAGCGTAATAGAGATA+AGG + chr1_2:60839193-60839212 Msa0082040:intron 30.0%
AGATGGTAAAAAATAGGCTT+AGG + chr1_2:60839312-60839331 Msa0082040:intron 30.0%
GATGAGAGTCAAATTATAAG+AGG + chr1_2:60839395-60839414 Msa0082040:intron 30.0%
AGTCAAATTATAAGAGGTAG+AGG + chr1_2:60839401-60839420 Msa0082040:intron 30.0%
AGATTTAGAGGTTAATGAGT+TGG + chr1_2:60839460-60839479 Msa0082040:intron 30.0%
! TAATGAGTTGGATCCAAATA+TGG + chr1_2:60839472-60839491 Msa0082040:intron 30.0%
ATAACATGTATAGGGCAAAA+TGG - chr1_2:60839611-60839630 None:intergenic 30.0%
TCATCTTGCAAACATAAGTT+TGG - chr1_2:60839772-60839791 None:intergenic 30.0%
TTAACCAGAGATATGATTCA+AGG - chr1_2:60839837-60839856 None:intergenic 30.0%
TTATTCATCTTTGCAAGCTT+TGG - chr1_2:60840044-60840063 None:intergenic 30.0%
GCAAAGATGAATAAGAAGTT+TGG + chr1_2:60840050-60840069 Msa0082040:CDS 30.0%
AAGAAGTTTGGTATGATTCA+TGG + chr1_2:60840062-60840081 Msa0082040:CDS 30.0%
AGAAGTTTGGTATGATTCAT+GGG + chr1_2:60840063-60840082 Msa0082040:CDS 30.0%
ACAAGTATCTCTACTAGAAT+GGG - chr1_2:60840184-60840203 None:intergenic 30.0%
!! ATTGATTTACCAAGAGTATC+CGG + chr1_2:60840245-60840264 Msa0082040:three_prime_UTR 30.0%
TCTAGAAACCGAAGATCATT+TGG + chr1_2:60837120-60837139 Msa0082040:five_prime_UTR 35.0%
TGGATTGACCAAATGATCTT+CGG - chr1_2:60837131-60837150 None:intergenic 35.0%
ATGAGGTAGTTGAATTGAAG+GGG - chr1_2:60837239-60837258 None:intergenic 35.0%
!! GATTTTGATCCGAAAGTTTC+TGG - chr1_2:60837367-60837386 None:intergenic 35.0%
ACGCTGAATTTCTTGTGTTT+AGG + chr1_2:60837571-60837590 Msa0082040:intron 35.0%
TTGTGTTTAGGAATTTGCCT+AGG + chr1_2:60837583-60837602 Msa0082040:intron 35.0%
AATTTGCCTAGGCATCAATT+TGG + chr1_2:60837594-60837613 Msa0082040:CDS 35.0%
AGATTACCAAATTGATGCCT+AGG - chr1_2:60837603-60837622 None:intergenic 35.0%
CCAACCATTGAAAAATAAGC+TGG - chr1_2:60837642-60837661 None:intergenic 35.0%
!! CCAGCTTATTTTTCAATGGT+TGG + chr1_2:60837639-60837658 Msa0082040:CDS 35.0%
!! AAGTTGGTATCTTTCACTAG+TGG - chr1_2:60837724-60837743 None:intergenic 35.0%
ACTAGTGAAAGATACCAACT+TGG + chr1_2:60837723-60837742 Msa0082040:CDS 35.0%
TGGTGTAAAGACGAACAAAT+TGG - chr1_2:60837781-60837800 None:intergenic 35.0%
CTTTACACCAATGACAATTG+AGG + chr1_2:60837791-60837810 Msa0082040:CDS 35.0%
TAGCTTAACACCTTATAGAG+AGG - chr1_2:60837973-60837992 None:intergenic 35.0%
CTTTAAGCTGGAAACTACTT+AGG + chr1_2:60838007-60838026 Msa0082040:intron 35.0%
AAGATATCCTGGAGATCTTA+TGG + chr1_2:60838139-60838158 Msa0082040:intron 35.0%
CTTATTTCCATAAGATCTCC+AGG - chr1_2:60838149-60838168 None:intergenic 35.0%
CTACAAGGGTCTATTTGTAT+TGG - chr1_2:60838266-60838285 None:intergenic 35.0%
! GGATGGAATTGAGTTTTTGA+AGG + chr1_2:60838495-60838514 Msa0082040:intron 35.0%
AGAAGATAGAACAGGAAATG+AGG - chr1_2:60838839-60838858 None:intergenic 35.0%
TGAACCAAAGAAGATAGAAC+AGG - chr1_2:60838847-60838866 None:intergenic 35.0%
!!! GTTCGATTTGTTTTTTGGTC+TGG + chr1_2:60838975-60838994 Msa0082040:intron 35.0%
TCCACAATGACAAAGGAAAA+AGG - chr1_2:60839035-60839054 None:intergenic 35.0%
TGAAATGTCCACAATGACAA+AGG - chr1_2:60839042-60839061 None:intergenic 35.0%
TGTTGTATGGTACGAAGTAT+TGG + chr1_2:60839145-60839164 Msa0082040:intron 35.0%
! GTTGTATGGTACGAAGTATT+GGG + chr1_2:60839146-60839165 Msa0082040:intron 35.0%
AGCACCTATAGTGTAAAAGA+TGG + chr1_2:60839295-60839314 Msa0082040:intron 35.0%
TGGTAAAAAATAGGCTTAGG+TGG + chr1_2:60839315-60839334 Msa0082040:intron 35.0%
GGTAAAAAATAGGCTTAGGT+GGG + chr1_2:60839316-60839335 Msa0082040:intron 35.0%
CTTCGTACTACAACATCTAT+CGG - chr1_2:60839362-60839381 None:intergenic 35.0%
GATCCAAATATGGTTTCAGA+TGG + chr1_2:60839482-60839501 Msa0082040:intron 35.0%
! GTTCCATCTGAAACCATATT+TGG - chr1_2:60839488-60839507 None:intergenic 35.0%
TGGTTTCAGATGGAACATTA+TGG + chr1_2:60839492-60839511 Msa0082040:intron 35.0%
CATACCTTGAATCATATCTC+TGG + chr1_2:60839830-60839849 Msa0082040:intron 35.0%
! GATGAAACAAAGGCATAAAG+TGG + chr1_2:60839953-60839972 Msa0082040:CDS 35.0%
! AATAGTATTGGAGAGGAAGT+TGG + chr1_2:60839984-60840003 Msa0082040:CDS 35.0%
! ATAGTATTGGAGAGGAAGTT+GGG + chr1_2:60839985-60840004 Msa0082040:CDS 35.0%
AAATGACATGATGTTGGCAA+GGG - chr1_2:60840096-60840115 None:intergenic 35.0%
GACAAGTATCTCTACTAGAA+TGG - chr1_2:60840185-60840204 None:intergenic 35.0%
! TGTACTATCAAAGCATCACA+CGG + chr1_2:60840221-60840240 Msa0082040:three_prime_UTR 35.0%
! TTTGACTACAGAAAGGTACA+TGG - chr1_2:60840347-60840366 None:intergenic 35.0%
ATGTACCTTTCTGTAGTCAA+AGG + chr1_2:60840346-60840365 Msa0082040:three_prime_UTR 35.0%
GTATACCTTTGACTACAGAA+AGG - chr1_2:60840354-60840373 None:intergenic 35.0%
TTAGCATGTGGGGAATAAAA+CGG + chr1_2:60840468-60840487 Msa0082040:three_prime_UTR 35.0%
!! GTTCTGAGAAAGGAGTAATG+AGG - chr1_2:60837256-60837275 None:intergenic 40.0%
TGGTGAGAAAATTACTCCGA+TGG - chr1_2:60837347-60837366 None:intergenic 40.0%
! TTTGAGGTAAGTGGAAAGAG+TGG - chr1_2:60837392-60837411 None:intergenic 40.0%
! CTAGTGGAAGAAGTTTTCCA+CGG - chr1_2:60837708-60837727 None:intergenic 40.0%
CAGAGATCAAGAAACCAAGT+TGG - chr1_2:60837740-60837759 None:intergenic 40.0%
ACGGGTATAACCTCTCTATA+AGG + chr1_2:60837960-60837979 Msa0082040:intron 40.0%
GAAACTACTTAGGCCATGTT+TGG + chr1_2:60838017-60838036 Msa0082040:intron 40.0%
AGACGTGTCATAAGATATCC+TGG + chr1_2:60838128-60838147 Msa0082040:intron 40.0%
! CAATTGAATCATCTAAGGCC+TGG + chr1_2:60838440-60838459 Msa0082040:intron 40.0%
! AGGAATCAACATTTAAGGCC+AGG - chr1_2:60838461-60838480 None:intergenic 40.0%
CCATCCCTCTAATTACTAAC+AGG - chr1_2:60838481-60838500 None:intergenic 40.0%
CCTGTTAGTAATTAGAGGGA+TGG + chr1_2:60838478-60838497 Msa0082040:intron 40.0%
AGCAAGCATCTTCGTTATTC+TGG - chr1_2:60838708-60838727 None:intergenic 40.0%
! GCCTTTTTCCTTTGTCATTG+TGG + chr1_2:60839031-60839050 Msa0082040:intron 40.0%
TAGAGATAAGGATGTTGCGT+TGG + chr1_2:60839205-60839224 Msa0082040:intron 40.0%
TGGTAACTGGTAAGACTAGA+CGG + chr1_2:60839233-60839252 Msa0082040:intron 40.0%
ATGACACCATTAGAGAGAGT+TGG + chr1_2:60839269-60839288 Msa0082040:intron 40.0%
TGACACCATTAGAGAGAGTT+GGG + chr1_2:60839270-60839289 Msa0082040:intron 40.0%
AAAAATAGGCTTAGGTGGGT+TGG + chr1_2:60839320-60839339 Msa0082040:intron 40.0%
! AAAATAGGCTTAGGTGGGTT+GGG + chr1_2:60839321-60839340 Msa0082040:intron 40.0%
AGTACGAAGAGTAGATCAGA+TGG + chr1_2:60839373-60839392 Msa0082040:intron 40.0%
TAGAAGTGAGCAGCAACTAT+TGG - chr1_2:60839890-60839909 None:intergenic 40.0%
GTGGAGAGAGAGAATAGTAT+TGG + chr1_2:60839972-60839991 Msa0082040:CDS 40.0%
! GAGAGAGAATAGTATTGGAG+AGG + chr1_2:60839977-60839996 Msa0082040:CDS 40.0%
CAAATGACATGATGTTGGCA+AGG - chr1_2:60840097-60840116 None:intergenic 40.0%
CTTGCCAACATCATGTCATT+TGG + chr1_2:60840095-60840114 Msa0082040:CDS 40.0%
GCTTCCAAATGACATGATGT+TGG - chr1_2:60840102-60840121 None:intergenic 40.0%
! AGTACCAAGAGTGTATTGCA+AGG - chr1_2:60840124-60840143 None:intergenic 40.0%
TGCAATACACTCTTGGTACT+TGG + chr1_2:60840124-60840143 Msa0082040:CDS 40.0%
AGTATCTCTACTAGAATGGG+AGG - chr1_2:60840181-60840200 None:intergenic 40.0%
GAGATACTTGTCTGTGCAAT+TGG + chr1_2:60840193-60840212 Msa0082040:three_prime_UTR 40.0%
! TTTCTCAGTGTGCTATTCTC+TGG + chr1_2:60840414-60840433 Msa0082040:three_prime_UTR 40.0%
!! TGGTGTTCTGGTTCTGAGAA+AGG - chr1_2:60837266-60837285 None:intergenic 45.0%
! AACCGCGGTTGTTATTAGAG+CGG - chr1_2:60837314-60837333 None:intergenic 45.0%
GATGGCTAAGAAAACAACCG+CGG - chr1_2:60837329-60837348 None:intergenic 45.0%
!! GCGGTTGTTTTCTTAGCCAT+CGG + chr1_2:60837328-60837347 Msa0082040:CDS 45.0%
! ATGAGCGAGTTTGAGGTAAG+TGG - chr1_2:60837401-60837420 None:intergenic 45.0%
TGTTTGCATTGCTGTCACTG+TGG + chr1_2:60837662-60837681 Msa0082040:CDS 45.0%
CCACGGATGAAGATAACCAA+AGG - chr1_2:60837691-60837710 None:intergenic 45.0%
CCTTTGGTTATCTTCATCCG+TGG + chr1_2:60837688-60837707 Msa0082040:CDS 45.0%
CTGCGAAGCTTACTTTAAGC+TGG + chr1_2:60837995-60838014 Msa0082040:intron 45.0%
AGACGGTTTGAGAGAGCTTA+CGG - chr1_2:60838208-60838227 None:intergenic 45.0%
CGGTATAAGTGCTCATGAGA+CGG - chr1_2:60838225-60838244 None:intergenic 45.0%
CGTCTCATGAGCACTTATAC+CGG + chr1_2:60838223-60838242 Msa0082040:intron 45.0%
TCATGAGCACTTATACCGGT+CGG + chr1_2:60838227-60838246 Msa0082040:intron 45.0%
ACAGGAAATGAGGACAGAAG+AGG - chr1_2:60838829-60838848 None:intergenic 45.0%
TGTTGCATCAGTGCAAGTTC+GGG - chr1_2:60838874-60838893 None:intergenic 45.0%
CTGTTGCATCAGTGCAAGTT+CGG - chr1_2:60838875-60838894 None:intergenic 45.0%
CTTCGTACCATACAACATCG+CGG - chr1_2:60839142-60839161 None:intergenic 45.0%
!! GTATGGTACGAAGTATTGGG+CGG + chr1_2:60839149-60839168 Msa0082040:intron 45.0%
CGCTTACTTGATTCTCGTGT+TGG - chr1_2:60839182-60839201 None:intergenic 45.0%
GGTAACTGGTAAGACTAGAC+GGG + chr1_2:60839234-60839253 Msa0082040:intron 45.0%
AGACTAGACGGGATAGGATT+AGG + chr1_2:60839245-60839264 Msa0082040:intron 45.0%
GACACCATTAGAGAGAGTTG+GGG + chr1_2:60839271-60839290 Msa0082040:intron 45.0%
AAGCATCATCCCACTAAGTG+GGG - chr1_2:60839541-60839560 None:intergenic 45.0%
CAAGCATCATCCCACTAAGT+GGG - chr1_2:60839542-60839561 None:intergenic 45.0%
GAAGCCTTGCAATACACTCT+TGG + chr1_2:60840117-60840136 Msa0082040:CDS 45.0%
ATACACTCTTGGTACTTGGC+AGG + chr1_2:60840128-60840147 Msa0082040:CDS 45.0%
ATGTTTGCACAGCAAGACAC+CGG - chr1_2:60840267-60840286 None:intergenic 45.0%
AGCCGCTCTAATAACAACCG+CGG + chr1_2:60837309-60837328 Msa0082040:CDS 50.0%
GAGGTAAGTGGAAAGAGTGG+TGG - chr1_2:60837389-60837408 None:intergenic 50.0%
CTCAAACTCGCTCATCTCCT+CGG + chr1_2:60837406-60837425 Msa0082040:CDS 50.0%
GATGAAGGTGACCCATAGAG+CGG - chr1_2:60837452-60837471 None:intergenic 50.0%
GCTCTATGGGTCACCTTCAT+CGG + chr1_2:60837451-60837470 Msa0082040:CDS 50.0%
CATGATGATACCGCCGATGA+AGG - chr1_2:60837467-60837486 None:intergenic 50.0%
! GGCTTTTTGAACTTGCCGAC+CGG - chr1_2:60838245-60838264 None:intergenic 50.0%
TTGCATCAGTGCAAGTTCGG+GGG - chr1_2:60838872-60838891 None:intergenic 50.0%
GTTGCATCAGTGCAAGTTCG+GGG - chr1_2:60838873-60838892 None:intergenic 50.0%
AGTCAGACCGCGATGTTGTA+TGG + chr1_2:60839132-60839151 Msa0082040:intron 50.0%
AGGATGTTGCGTTGGATGAG+TGG + chr1_2:60839213-60839232 Msa0082040:intron 50.0%
TGCGTTGGATGAGTGGTAAC+TGG + chr1_2:60839220-60839239 Msa0082040:intron 50.0%
CTGGTAAGACTAGACGGGAT+AGG + chr1_2:60839239-60839258 Msa0082040:intron 50.0%
GCTACCCCAACTCTCTCTAA+TGG - chr1_2:60839278-60839297 None:intergenic 50.0%
ATCATCCCACTAAGTGGGGT+CGG - chr1_2:60839537-60839556 None:intergenic 50.0%
CCAAGCATCATCCCACTAAG+TGG - chr1_2:60839543-60839562 None:intergenic 50.0%
CCACTTAGTGGGATGATGCT+TGG + chr1_2:60839540-60839559 Msa0082040:intron 50.0%
GTATTGGAGAGGAAGTTGGG+TGG + chr1_2:60839988-60840007 Msa0082040:CDS 50.0%
AGCAAGACACCGGATACTCT+TGG - chr1_2:60840257-60840276 None:intergenic 50.0%
AACCAGATTCCAGCGCTCGT+GGG - chr1_2:60837156-60837175 None:intergenic 55.0%
AAACCAGATTCCAGCGCTCG+TGG - chr1_2:60837157-60837176 None:intergenic 55.0%
!! ACGAGCGCTGGAATCTGGTT+TGG + chr1_2:60837156-60837175 Msa0082040:five_prime_UTR 55.0%
ACCACCACCACCGTCATCAA+TGG + chr1_2:60837282-60837301 Msa0082040:exon 55.0%
CCACCACCACCGTCATCAAT+GGG + chr1_2:60837283-60837302 Msa0082040:exon 55.0%
!! CTACCCATTGATGACGGTGG+TGG - chr1_2:60837289-60837308 None:intergenic 55.0%
!! GCTCTACCCATTGATGACGG+TGG - chr1_2:60837292-60837311 None:intergenic 55.0%
GCGGCTCTACCCATTGATGA+CGG - chr1_2:60837295-60837314 None:intergenic 55.0%
CGAGGAGATGAGCGAGTTTG+AGG - chr1_2:60837408-60837427 None:intergenic 55.0%
CTCGGCTTCTCCATCGCTTT+CGG + chr1_2:60837424-60837443 Msa0082040:CDS 55.0%
CTATGGGTCACCTTCATCGG+CGG + chr1_2:60837454-60837473 Msa0082040:CDS 55.0%
GCTGTCACTGTGGCTTCCTT+TGG + chr1_2:60837672-60837691 Msa0082040:CDS 55.0%
ACTAAGTGGGGTCGGCTACA+TGG - chr1_2:60839529-60839548 None:intergenic 55.0%
ATGTAGCCGACCCCACTTAG+TGG + chr1_2:60839528-60839547 Msa0082040:intron 55.0%
TGTAGCCGACCCCACTTAGT+GGG + chr1_2:60839529-60839548 Msa0082040:intron 55.0%
CAATCCAAACCCACGAGCGC+TGG + chr1_2:60837144-60837163 Msa0082040:five_prime_UTR 60.0%
GATTCCAGCGCTCGTGGGTT+TGG - chr1_2:60837151-60837170 None:intergenic 60.0%
!! AACCCACGAGCGCTGGAATC+TGG + chr1_2:60837151-60837170 Msa0082040:five_prime_UTR 60.0%
!! TGACGGTGGTGGTGGTGTTC+TGG - chr1_2:60837278-60837297 None:intergenic 60.0%
!! CCCATTGATGACGGTGGTGG+TGG - chr1_2:60837286-60837305 None:intergenic 60.0%
CGAAAGCGATGGAGAAGCCG+AGG - chr1_2:60837426-60837445 None:intergenic 60.0%
! TAGAGCGGCGCCGAAAGCGA+TGG - chr1_2:60837437-60837456 None:intergenic 65.0%
TCGCTTTCGGCGCCGCTCTA+TGG + chr1_2:60837437-60837456 Msa0082040:CDS 65.0%
CGCTTTCGGCGCCGCTCTAT+GGG + chr1_2:60837438-60837457 Msa0082040:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 60837086 60840504 60837086 ID=Msa0082040;Name=Msa0082040
chr1_2 mRNA 60837086 60840504 60837086 ID=Msa0082040-mRNA-1;Parent=Msa0082040;Name=Msa0082040-mRNA-1;_AED=0.06;_eAED=0.06;_QI=215|1|1|1|1|1|3|338|208
chr1_2 exon 60837086 60837491 60837086 ID=Msa0082040-mRNA-1:exon:19869;Parent=Msa0082040-mRNA-1
chr1_2 exon 60837593 60837812 60837593 ID=Msa0082040-mRNA-1:exon:19870;Parent=Msa0082040-mRNA-1
chr1_2 exon 60839951 60840504 60839951 ID=Msa0082040-mRNA-1:exon:19871;Parent=Msa0082040-mRNA-1
chr1_2 five_prime_UTR 60837086 60837300 60837086 ID=Msa0082040-mRNA-1:five_prime_utr;Parent=Msa0082040-mRNA-1
chr1_2 CDS 60837301 60837491 60837301 ID=Msa0082040-mRNA-1:cds;Parent=Msa0082040-mRNA-1
chr1_2 CDS 60837593 60837812 60837593 ID=Msa0082040-mRNA-1:cds;Parent=Msa0082040-mRNA-1
chr1_2 CDS 60839951 60840166 60839951 ID=Msa0082040-mRNA-1:cds;Parent=Msa0082040-mRNA-1
chr1_2 three_prime_UTR 60840167 60840504 60840167 ID=Msa0082040-mRNA-1:three_prime_utr;Parent=Msa0082040-mRNA-1
Gene Sequence

>Msa0082040

ATGGGTAGAGCCGCTCTAATAACAACCGCGGTTGTTTTCTTAGCCATCGGAGTAATTTTCTCACCAGAAACTTTCGGATCAAAATCCACCACTCTTTCCACTTACCTCAAACTCGCTCATCTCCTCGGCTTCTCCATCGCTTTCGGCGCCGCTCTATGGGTCACCTTCATCGGCGGTATCATCATGTTCAAGAATTTGCCTAGGCATCAATTTGGTAATCTTCAAAGCAAAATGTTTCCAGCTTATTTTTCAATGGTTGGTGTTTGCATTGCTGTCACTGTGGCTTCCTTTGGTTATCTTCATCCGTGGAAAACTTCTTCCACTAGTGAAAGATACCAACTTGGTTTCTTGATCTCTGCTCTTGCTTTCAATCTTACCAATTTGTTCGTCTTTACACCAATGACAATTGAGATGATGAAACAAAGGCATAAAGTGGAGAGAGAGAATAGTATTGGAGAGGAAGTTGGGTGGTCAAAAAATGTCGAAGTTGCAAAGAAAAATCCAAAGCTTGCAAAGATGAATAAGAAGTTTGGTATGATTCATGGGTTATCTTCCCTTGCCAACATCATGTCATTTGGAAGCCTTGCAATACACTCTTGGTACTTGGCAGGTAAACTTGATCTCTGA
Protein sequence

>Msa0082040

MGRAALITTAVVFLAIGVIFSPETFGSKSTTLSTYLKLAHLLGFSIAFGAALWVTFIGGIIMFKNLPRHQFGNLQSKMFPAYFSMVGVCIAVTVASFGYLHPWKTSSTSERYQLGFLISALAFNLTNLFVFTPMTIEMMKQRHKVERENSIGEEVGWSKNVEVAKKNPKLAKMNKKFGMIHGLSSLANIMSFGSLAIHSWYLAGKLDL*