AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0583680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0583680 MtrunA17_Chr1g0194891 98.558 208 3 0 1 208 1 208 7.54e-152 419
Msa0583680 MtrunA17_Chr4g0014721 29.268 123 87 0 32 154 228 350 4.45e-15 73.2
Msa0583680 MtrunA17_Chr4g0014741 29.268 123 87 0 32 154 228 350 4.50e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0583680 AT3G62580.1 74.648 213 49 1 1 208 1 213 1.20e-115 328
Msa0583680 AT1G72100.1 28.723 188 121 4 25 206 296 476 9.96e-14 69.7
Msa0583680 AT1G22600.1 28.342 187 114 4 41 208 198 383 1.01e-11 63.5

Find 85 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGCTTTCGGCGCCGCTCTA+TGG 0.164177 4_2:-2375851 Msa0583680:CDS
TTATTCATCTTTGCAAGCTT+TGG 0.242253 4_2:+2373247 None:intergenic
GATTCCAGCGCTCGTGGGTT+TGG 0.328551 4_2:+2376140 None:intergenic
CAATATGGGGCAGCTGTATT+AGG 0.336814 4_2:-2376206 None:intergenic
CTTGCCAACATCATGTCATT+TGG 0.339373 4_2:-2373193 Msa0583680:CDS
CGCTTTCGGCGCCGCTCTAT+GGG 0.344664 4_2:-2375850 Msa0583680:CDS
AATAGTATTGGAGAGGAAGT+TGG 0.347347 4_2:-2373304 Msa0583680:CDS
AAGAAGTTTGGTATGATTCA+TGG 0.351606 4_2:-2373226 Msa0583680:CDS
CTCGGCTTCTCCATCGCTTT+CGG 0.353954 4_2:-2375864 Msa0583680:CDS
AATTTGCCTAGGCATCAATT+TGG 0.358606 4_2:-2375694 Msa0583680:CDS
GCTGTCACTGTGGCTTCCTT+TGG 0.364294 4_2:-2375616 Msa0583680:CDS
TTTCTCAGTGTGCTATTCTC+TGG 0.370714 4_2:-2372874 Msa0583680:three_prime_UTR
TGACGGTGGTGGTGGTGTTC+TGG 0.374589 4_2:+2376013 None:intergenic
AACCCACGAGCGCTGGAATC+TGG 0.377154 4_2:-2376137 Msa0583680:five_prime_UTR
GCAAAGATGAATAAGAAGTT+TGG 0.383264 4_2:-2373238 Msa0583680:CDS
ATTATGCTATAAATTATATA+AGG 0.396138 4_2:-2372910 Msa0583680:three_prime_UTR
TGCAATACACTCTTGGTACT+TGG 0.405780 4_2:-2373164 Msa0583680:CDS
TGGTGTAAAGACGAACAAAT+TGG 0.408776 4_2:+2375510 None:intergenic
TCTAGAAACCGAAGATCATT+TGG 0.420817 4_2:-2376168 Msa0583680:five_prime_UTR
ATAGTATTGGAGAGGAAGTT+GGG 0.421614 4_2:-2373303 Msa0583680:CDS
CGAGGAGATGAGCGAGTTTG+AGG 0.436447 4_2:+2375883 None:intergenic
TGGATTGACCAAATGATCTT+CGG 0.439017 4_2:+2376160 None:intergenic
GACAAGTATCTCTACTAGAA+TGG 0.440265 4_2:+2373106 None:intergenic
GTGGAGAGAGAGAATAGTAT+TGG 0.444526 4_2:-2373316 Msa0583680:CDS
TAATGAGGTAGTTGAATTGA+AGG 0.451077 4_2:+2376050 None:intergenic
GAGATACTTGTCTGTGCAAT+TGG 0.459576 4_2:-2373095 Msa0583680:three_prime_UTR
CAATCCAAACCCACGAGCGC+TGG 0.468537 4_2:-2376144 Msa0583680:five_prime_UTR
ACAAGTATCTCTACTAGAAT+GGG 0.473721 4_2:+2373107 None:intergenic
AGAAGTTTGGTATGATTCAT+GGG 0.477765 4_2:-2373225 Msa0583680:CDS
GAAAATACCTCAATTGTCAT+TGG 0.481213 4_2:+2375490 None:intergenic
ATGAGCGAGTTTGAGGTAAG+TGG 0.481460 4_2:+2375890 None:intergenic
GAAGCCTTGCAATACACTCT+TGG 0.483767 4_2:-2373171 Msa0583680:CDS
GCTCTATGGGTCACCTTCAT+CGG 0.492086 4_2:-2375837 Msa0583680:CDS
ACGAGCGCTGGAATCTGGTT+TGG 0.492118 4_2:-2376132 Msa0583680:five_prime_UTR
ATTGATTTACCAAGAGTATC+CGG 0.495468 4_2:-2373043 Msa0583680:three_prime_UTR
ACCACCACCACCGTCATCAA+TGG 0.497424 4_2:-2376006 Msa0583680:exon
AGATTACCAAATTGATGCCT+AGG 0.499514 4_2:+2375688 None:intergenic
TGGTGTTCTGGTTCTGAGAA+AGG 0.511223 4_2:+2376025 None:intergenic
AATGAGGTAGTTGAATTGAA+GGG 0.512893 4_2:+2376051 None:intergenic
CCACCACCACCGTCATCAAT+GGG 0.515355 4_2:-2376005 Msa0583680:exon
CTCAAACTCGCTCATCTCCT+CGG 0.520112 4_2:-2375882 Msa0583680:CDS
GATGAAACAAAGGCATAAAG+TGG 0.520254 4_2:-2373335 Msa0583680:CDS
CCCATTGATGACGGTGGTGG+TGG 0.521011 4_2:+2376005 None:intergenic
CTTTACACCAATGACAATTG+AGG 0.521289 4_2:-2375497 Msa0583680:intron
ATACACTCTTGGTACTTGGC+AGG 0.533564 4_2:-2373160 Msa0583680:CDS
AGCAAGACACCGGATACTCT+TGG 0.534506 4_2:+2373034 None:intergenic
GAGAGAGAATAGTATTGGAG+AGG 0.541841 4_2:-2373311 Msa0583680:CDS
TTGTGTTTAGGAATTTGCCT+AGG 0.542157 4_2:-2375705 Msa0583680:intron
TGGTGAGAAAATTACTCCGA+TGG 0.542306 4_2:+2375944 None:intergenic
TTAGCATGTGGGGAATAAAA+CGG 0.543297 4_2:-2372820 Msa0583680:three_prime_UTR
AAACCAGATTCCAGCGCTCG+TGG 0.544546 4_2:+2376134 None:intergenic
TAGAGCGGCGCCGAAAGCGA+TGG 0.548120 4_2:+2375854 None:intergenic
CAAATGACATGATGTTGGCA+AGG 0.567231 4_2:+2373194 None:intergenic
CATGATGATACCGCCGATGA+AGG 0.570088 4_2:+2375824 None:intergenic
AAGTTGGTATCTTTCACTAG+TGG 0.574244 4_2:+2375567 None:intergenic
CGAAAGCGATGGAGAAGCCG+AGG 0.581248 4_2:+2375865 None:intergenic
ATGAGGTAGTTGAATTGAAG+GGG 0.581274 4_2:+2376052 None:intergenic
TTTGAGGTAAGTGGAAAGAG+TGG 0.582513 4_2:+2375899 None:intergenic
ATGTACCTTTCTGTAGTCAA+AGG 0.582953 4_2:-2372942 Msa0583680:three_prime_UTR
GAGGTAAGTGGAAAGAGTGG+TGG 0.583033 4_2:+2375902 None:intergenic
TTTGACTACAGAAAGGTACA+TGG 0.584102 4_2:+2372944 None:intergenic
ACTAGTGAAAGATACCAACT+TGG 0.589922 4_2:-2375565 Msa0583680:CDS
CCTTTGGTTATCTTCATCCG+TGG 0.593206 4_2:-2375600 Msa0583680:CDS
GCTTCCAAATGACATGATGT+TGG 0.594501 4_2:+2373189 None:intergenic
CAGAGATCAAGAAACCAAGT+TGG 0.596357 4_2:+2375551 None:intergenic
AAATGACATGATGTTGGCAA+GGG 0.609102 4_2:+2373195 None:intergenic
AACCAGATTCCAGCGCTCGT+GGG 0.615154 4_2:+2376135 None:intergenic
TGATATAGATGATGAAACAA+AGG 0.622671 4_2:-2373345 Msa0583680:intron
AGTATCTCTACTAGAATGGG+AGG 0.623701 4_2:+2373110 None:intergenic
CCACGGATGAAGATAACCAA+AGG 0.626168 4_2:+2375600 None:intergenic
ATGTTTGCACAGCAAGACAC+CGG 0.626366 4_2:+2373024 None:intergenic
CTACCCATTGATGACGGTGG+TGG 0.630995 4_2:+2376002 None:intergenic
GCGGCTCTACCCATTGATGA+CGG 0.643113 4_2:+2375996 None:intergenic
CTATGGGTCACCTTCATCGG+CGG 0.646660 4_2:-2375834 Msa0583680:CDS
GTATACCTTTGACTACAGAA+AGG 0.656498 4_2:+2372937 None:intergenic
AACCGCGGTTGTTATTAGAG+CGG 0.674556 4_2:+2375977 None:intergenic
GATGAAGGTGACCCATAGAG+CGG 0.683702 4_2:+2375839 None:intergenic
GTTCTGAGAAAGGAGTAATG+AGG 0.684686 4_2:+2376035 None:intergenic
AGTACCAAGAGTGTATTGCA+AGG 0.685013 4_2:+2373167 None:intergenic
TGTTTGCATTGCTGTCACTG+TGG 0.686509 4_2:-2375626 Msa0583680:CDS
TGTACTATCAAAGCATCACA+CGG 0.689750 4_2:-2373067 Msa0583680:three_prime_UTR
GCTCTACCCATTGATGACGG+TGG 0.693373 4_2:+2375999 None:intergenic
GTATTGGAGAGGAAGTTGGG+TGG 0.710494 4_2:-2373300 Msa0583680:CDS
AGCCGCTCTAATAACAACCG+CGG 0.732310 4_2:-2375979 Msa0583680:CDS
GATGGCTAAGAAAACAACCG+CGG 0.788667 4_2:+2375962 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATTAAATTAACATAATTA+GGG + chr4_2:2373255-2373274 None:intergenic 10.0%
!! ATTATGCTATAAATTATATA+AGG - chr4_2:2376098-2376117 Msa0583680:five_prime_UTR 10.0%
!! GATTAAATTAACATAATTAG+GGG + chr4_2:2373254-2373273 None:intergenic 15.0%
!! CTGATTAAATTAACATAATT+AGG + chr4_2:2373256-2373275 None:intergenic 15.0%
!!! TATTTTATTCAAGTAAACTA+AGG + chr4_2:2376038-2376057 None:intergenic 15.0%
!!! TTCTTTTCTTTGTCTTTATA+AGG - chr4_2:2373560-2373579 Msa0583680:intron 20.0%
!!! ATGTTTATAGAATTTTGACA+TGG + chr4_2:2374124-2374143 None:intergenic 20.0%
!! AAATGAAAGAATCAAGAAAA+GGG + chr4_2:2374327-2374346 None:intergenic 20.0%
!! AAAATGAAAGAATCAAGAAA+AGG + chr4_2:2374328-2374347 None:intergenic 20.0%
!! ATTACTTTACCAAATATGTA+TGG - chr4_2:2374369-2374388 Msa0583680:intron 20.0%
!! AATATTTCACCATACATATT+TGG + chr4_2:2374381-2374400 None:intergenic 20.0%
!! AATTTGCCTTTAAATTTAAG+CGG + chr4_2:2374820-2374839 None:intergenic 20.0%
!! TGTAAAAGATGGTAAAAAAT+AGG - chr4_2:2375026-2375045 Msa0583680:intron 20.0%
!! AACTATTAGAAAAGATTTAG+AGG - chr4_2:2375168-2375187 Msa0583680:intron 20.0%
!! AATATAGCATAACATGTATA+GGG + chr4_2:2375339-2375358 None:intergenic 20.0%
!! TAATATAGCATAACATGTAT+AGG + chr4_2:2375340-2375359 None:intergenic 20.0%
!! TTGTAACATGATTTATGTTA+GGG + chr4_2:2375454-2375473 None:intergenic 20.0%
!! TCTAATAAAAAATTAGCATG+TGG - chr4_2:2376176-2376195 Msa0583680:five_prime_UTR 20.0%
!! CTAATAAAAAATTAGCATGT+GGG - chr4_2:2376177-2376196 Msa0583680:five_prime_UTR 20.0%
!! TAATAAAAAATTAGCATGTG+GGG - chr4_2:2376178-2376197 Msa0583680:five_prime_UTR 20.0%
! GAAATTGATTAATTGATGGA+TGG + chr4_2:2373232-2373251 None:intergenic 25.0%
! AGAAGAATAATGAGAAAAGA+CGG - chr4_2:2373661-2373680 Msa0583680:intron 25.0%
!!! ACCTATGCATAAGTTTTATT+AGG - chr4_2:2373779-2373798 Msa0583680:intron 25.0%
!!! CCTATGCATAAGTTTTATTA+GGG - chr4_2:2373780-2373799 Msa0583680:intron 25.0%
! GATTATAGATATCTCTACAA+GGG + chr4_2:2374000-2374019 None:intergenic 25.0%
!! CTTGTATTGCATAAGTTTTT+AGG - chr4_2:2374446-2374465 Msa0583680:intron 25.0%
! AAAAAATATACCGATGGTTA+AGG + chr4_2:2374483-2374502 None:intergenic 25.0%
! CAATTCAAAAAATATACCGA+TGG + chr4_2:2374489-2374508 None:intergenic 25.0%
! AACAATAACAACAAATGTTC+AGG - chr4_2:2374632-2374651 Msa0583680:intron 25.0%
! CAAAAAACAAATCGAACAAA+AGG + chr4_2:2374692-2374711 None:intergenic 25.0%
!!! CTTTTGTTCGATTTGTTTTT+TGG - chr4_2:2374690-2374709 Msa0583680:intron 25.0%
! TACTTTCATATTGCATGTAT+AGG + chr4_2:2374797-2374816 None:intergenic 25.0%
!!! AAAGTACCGCTTAAATTTAA+AGG - chr4_2:2374811-2374830 Msa0583680:intron 25.0%
!! TTTACCATCTTTTACACTAT+AGG + chr4_2:2375022-2375041 None:intergenic 25.0%
! TACAATACTCATAACATTGT+CGG - chr4_2:2375363-2375382 Msa0583680:intron 25.0%
! GTTGTAACATGATTTATGTT+AGG + chr4_2:2375455-2375474 None:intergenic 25.0%
! TAAAGTTTGAAAACTTACGT+TGG + chr4_2:2375523-2375542 None:intergenic 25.0%
! TCTATATCAGACAAACAAAA+TGG + chr4_2:2375652-2375671 None:intergenic 25.0%
! TGATATAGATGATGAAACAA+AGG - chr4_2:2375663-2375682 Msa0583680:CDS 25.0%
AATGAGGTAGTTGAATTGAA+GGG + chr4_2:2372960-2372979 None:intergenic 30.0%
TAATGAGGTAGTTGAATTGA+AGG + chr4_2:2372961-2372980 None:intergenic 30.0%
! ATTTTCTCACCAGAAACTTT+CGG - chr4_2:2373075-2373094 Msa0583680:three_prime_UTR 30.0%
AGGGGAAATTGATTAATTGA+TGG + chr4_2:2373236-2373255 None:intergenic 30.0%
!! GTTTCCAGCTTATTTTTCAA+TGG - chr4_2:2373355-2373374 Msa0583680:intron 30.0%
GAAAATACCTCAATTGTCAT+TGG + chr4_2:2373521-2373540 None:intergenic 30.0%
GAAGAATAATGAGAAAAGAC+GGG - chr4_2:2373662-2373681 Msa0583680:intron 30.0%
GTAAGTTATTCATCCAAACA+TGG + chr4_2:2373753-2373772 None:intergenic 30.0%
CCCTAATAAAACTTATGCAT+AGG + chr4_2:2373783-2373802 None:intergenic 30.0%
CGATTATAGATATCTCTACA+AGG + chr4_2:2374001-2374020 None:intergenic 30.0%
!!! GATAAGCTTTTTCAAACACT+AGG - chr4_2:2374020-2374039 Msa0583680:intron 30.0%
! GTAAGCTCAAATAAGCTTTT+TGG - chr4_2:2374058-2374077 Msa0583680:intron 30.0%
TCTTGCAATTGAATCATCTA+AGG - chr4_2:2374155-2374174 Msa0583680:intron 30.0%
CTAACAGGAATCAACATTTA+AGG + chr4_2:2374186-2374205 None:intergenic 30.0%
TGATTCCTGTTAGTAATTAG+AGG - chr4_2:2374193-2374212 Msa0583680:intron 30.0%
GATTCCTGTTAGTAATTAGA+GGG - chr4_2:2374194-2374213 Msa0583680:intron 30.0%
! CTAAATGTTCTCTTTTGTGT+TGG - chr4_2:2374249-2374268 Msa0583680:intron 30.0%
! GTAATGTTTTCCTTAACCAT+CGG - chr4_2:2374470-2374489 Msa0583680:intron 30.0%
ATTTCCTGTTCTATCTTCTT+TGG - chr4_2:2374560-2374579 Msa0583680:intron 30.0%
AAATCAGTCAACACAAAAGT+AGG - chr4_2:2374722-2374741 Msa0583680:intron 30.0%
AATCAGTCAACACAAAAGTA+GGG - chr4_2:2374723-2374742 Msa0583680:intron 30.0%
TATTGCATGTATAGGCAAAA+TGG + chr4_2:2374789-2374808 None:intergenic 30.0%
AAGTAAGCGTAATAGAGATA+AGG - chr4_2:2374913-2374932 Msa0583680:intron 30.0%
AGATGGTAAAAAATAGGCTT+AGG - chr4_2:2375032-2375051 Msa0583680:intron 30.0%
GATGAGAGTCAAATTATAAG+AGG - chr4_2:2375115-2375134 Msa0583680:intron 30.0%
AGTCAAATTATAAGAGGTAG+AGG - chr4_2:2375121-2375140 Msa0583680:intron 30.0%
AGATTTAGAGGTTAATGAGT+TGG - chr4_2:2375180-2375199 Msa0583680:intron 30.0%
! TAATGAGTTGGATCCAAATA+TGG - chr4_2:2375192-2375211 Msa0583680:intron 30.0%
ATAACATGTATAGGGCAAAA+TGG + chr4_2:2375331-2375350 None:intergenic 30.0%
TCATCTTGCAAACATAAGTT+TGG + chr4_2:2375492-2375511 None:intergenic 30.0%
TTAACCAGAGATATGATTCA+AGG + chr4_2:2375557-2375576 None:intergenic 30.0%
TTATTCATCTTTGCAAGCTT+TGG + chr4_2:2375764-2375783 None:intergenic 30.0%
GCAAAGATGAATAAGAAGTT+TGG - chr4_2:2375770-2375789 Msa0583680:intron 30.0%
AAGAAGTTTGGTATGATTCA+TGG - chr4_2:2375782-2375801 Msa0583680:intron 30.0%
AGAAGTTTGGTATGATTCAT+GGG - chr4_2:2375783-2375802 Msa0583680:intron 30.0%
ACAAGTATCTCTACTAGAAT+GGG + chr4_2:2375904-2375923 None:intergenic 30.0%
!! ATTGATTTACCAAGAGTATC+CGG - chr4_2:2375965-2375984 Msa0583680:CDS 30.0%
TCTAGAAACCGAAGATCATT+TGG - chr4_2:2372840-2372859 Msa0583680:three_prime_UTR 35.0%
TGGATTGACCAAATGATCTT+CGG + chr4_2:2372851-2372870 None:intergenic 35.0%
ATGAGGTAGTTGAATTGAAG+GGG + chr4_2:2372959-2372978 None:intergenic 35.0%
!! GATTTTGATCCGAAAGTTTC+TGG + chr4_2:2373087-2373106 None:intergenic 35.0%
ACGCTGAATTTCTTGTGTTT+AGG - chr4_2:2373291-2373310 Msa0583680:CDS 35.0%
TTGTGTTTAGGAATTTGCCT+AGG - chr4_2:2373303-2373322 Msa0583680:CDS 35.0%
AATTTGCCTAGGCATCAATT+TGG - chr4_2:2373314-2373333 Msa0583680:CDS 35.0%
AGATTACCAAATTGATGCCT+AGG + chr4_2:2373323-2373342 None:intergenic 35.0%
CCAACCATTGAAAAATAAGC+TGG + chr4_2:2373362-2373381 None:intergenic 35.0%
!! CCAGCTTATTTTTCAATGGT+TGG - chr4_2:2373359-2373378 Msa0583680:intron 35.0%
!! AAGTTGGTATCTTTCACTAG+TGG + chr4_2:2373444-2373463 None:intergenic 35.0%
ACTAGTGAAAGATACCAACT+TGG - chr4_2:2373443-2373462 Msa0583680:intron 35.0%
TGGTGTAAAGACGAACAAAT+TGG + chr4_2:2373501-2373520 None:intergenic 35.0%
CTTTACACCAATGACAATTG+AGG - chr4_2:2373511-2373530 Msa0583680:intron 35.0%
TAGCTTAACACCTTATAGAG+AGG + chr4_2:2373693-2373712 None:intergenic 35.0%
CTTTAAGCTGGAAACTACTT+AGG - chr4_2:2373727-2373746 Msa0583680:intron 35.0%
AAGATATCCTGGAGATCTTA+TGG - chr4_2:2373859-2373878 Msa0583680:intron 35.0%
CTTATTTCCATAAGATCTCC+AGG + chr4_2:2373869-2373888 None:intergenic 35.0%
CTACAAGGGTCTATTTGTAT+TGG + chr4_2:2373986-2374005 None:intergenic 35.0%
! GGATGGAATTGAGTTTTTGA+AGG - chr4_2:2374215-2374234 Msa0583680:intron 35.0%
AGAAGATAGAACAGGAAATG+AGG + chr4_2:2374559-2374578 None:intergenic 35.0%
TGAACCAAAGAAGATAGAAC+AGG + chr4_2:2374567-2374586 None:intergenic 35.0%
!!! GTTCGATTTGTTTTTTGGTC+TGG - chr4_2:2374695-2374714 Msa0583680:intron 35.0%
TCCACAATGACAAAGGAAAA+AGG + chr4_2:2374755-2374774 None:intergenic 35.0%
TGAAATGTCCACAATGACAA+AGG + chr4_2:2374762-2374781 None:intergenic 35.0%
TGTTGTATGGTACGAAGTAT+TGG - chr4_2:2374865-2374884 Msa0583680:intron 35.0%
! GTTGTATGGTACGAAGTATT+GGG - chr4_2:2374866-2374885 Msa0583680:intron 35.0%
AGCACCTATAGTGTAAAAGA+TGG - chr4_2:2375015-2375034 Msa0583680:intron 35.0%
TGGTAAAAAATAGGCTTAGG+TGG - chr4_2:2375035-2375054 Msa0583680:intron 35.0%
GGTAAAAAATAGGCTTAGGT+GGG - chr4_2:2375036-2375055 Msa0583680:intron 35.0%
CTTCGTACTACAACATCTAT+CGG + chr4_2:2375082-2375101 None:intergenic 35.0%
GATCCAAATATGGTTTCAGA+TGG - chr4_2:2375202-2375221 Msa0583680:intron 35.0%
! GTTCCATCTGAAACCATATT+TGG + chr4_2:2375208-2375227 None:intergenic 35.0%
TGGTTTCAGATGGAACATTA+TGG - chr4_2:2375212-2375231 Msa0583680:intron 35.0%
CATACCTTGAATCATATCTC+TGG - chr4_2:2375550-2375569 Msa0583680:CDS 35.0%
! GATGAAACAAAGGCATAAAG+TGG - chr4_2:2375673-2375692 Msa0583680:CDS 35.0%
! AATAGTATTGGAGAGGAAGT+TGG - chr4_2:2375704-2375723 Msa0583680:intron 35.0%
! ATAGTATTGGAGAGGAAGTT+GGG - chr4_2:2375705-2375724 Msa0583680:intron 35.0%
AAATGACATGATGTTGGCAA+GGG + chr4_2:2375816-2375835 None:intergenic 35.0%
GACAAGTATCTCTACTAGAA+TGG + chr4_2:2375905-2375924 None:intergenic 35.0%
! TGTACTATCAAAGCATCACA+CGG - chr4_2:2375941-2375960 Msa0583680:CDS 35.0%
! TTTGACTACAGAAAGGTACA+TGG + chr4_2:2376067-2376086 None:intergenic 35.0%
ATGTACCTTTCTGTAGTCAA+AGG - chr4_2:2376066-2376085 Msa0583680:five_prime_UTR 35.0%
GTATACCTTTGACTACAGAA+AGG + chr4_2:2376074-2376093 None:intergenic 35.0%
TTAGCATGTGGGGAATAAAA+CGG - chr4_2:2376188-2376207 Msa0583680:five_prime_UTR 35.0%
!! GTTCTGAGAAAGGAGTAATG+AGG + chr4_2:2372976-2372995 None:intergenic 40.0%
TGGTGAGAAAATTACTCCGA+TGG + chr4_2:2373067-2373086 None:intergenic 40.0%
! TTTGAGGTAAGTGGAAAGAG+TGG + chr4_2:2373112-2373131 None:intergenic 40.0%
! CTAGTGGAAGAAGTTTTCCA+CGG + chr4_2:2373428-2373447 None:intergenic 40.0%
CAGAGATCAAGAAACCAAGT+TGG + chr4_2:2373460-2373479 None:intergenic 40.0%
ACGGGTATAACCTCTCTATA+AGG - chr4_2:2373680-2373699 Msa0583680:intron 40.0%
GAAACTACTTAGGCCATGTT+TGG - chr4_2:2373737-2373756 Msa0583680:intron 40.0%
AGACGTGTCATAAGATATCC+TGG - chr4_2:2373848-2373867 Msa0583680:intron 40.0%
! CAATTGAATCATCTAAGGCC+TGG - chr4_2:2374160-2374179 Msa0583680:intron 40.0%
! AGGAATCAACATTTAAGGCC+AGG + chr4_2:2374181-2374200 None:intergenic 40.0%
CCATCCCTCTAATTACTAAC+AGG + chr4_2:2374201-2374220 None:intergenic 40.0%
CCTGTTAGTAATTAGAGGGA+TGG - chr4_2:2374198-2374217 Msa0583680:intron 40.0%
AGCAAGCATCTTCGTTATTC+TGG + chr4_2:2374428-2374447 None:intergenic 40.0%
! GCCTTTTTCCTTTGTCATTG+TGG - chr4_2:2374751-2374770 Msa0583680:intron 40.0%
TAGAGATAAGGATGTTGCGT+TGG - chr4_2:2374925-2374944 Msa0583680:intron 40.0%
TGGTAACTGGTAAGACTAGA+CGG - chr4_2:2374953-2374972 Msa0583680:intron 40.0%
ATGACACCATTAGAGAGAGT+TGG - chr4_2:2374989-2375008 Msa0583680:intron 40.0%
TGACACCATTAGAGAGAGTT+GGG - chr4_2:2374990-2375009 Msa0583680:intron 40.0%
AAAAATAGGCTTAGGTGGGT+TGG - chr4_2:2375040-2375059 Msa0583680:intron 40.0%
! AAAATAGGCTTAGGTGGGTT+GGG - chr4_2:2375041-2375060 Msa0583680:intron 40.0%
AGTACGAAGAGTAGATCAGA+TGG - chr4_2:2375093-2375112 Msa0583680:intron 40.0%
TAGAAGTGAGCAGCAACTAT+TGG + chr4_2:2375610-2375629 None:intergenic 40.0%
GTGGAGAGAGAGAATAGTAT+TGG - chr4_2:2375692-2375711 Msa0583680:CDS 40.0%
! GAGAGAGAATAGTATTGGAG+AGG - chr4_2:2375697-2375716 Msa0583680:CDS 40.0%
CAAATGACATGATGTTGGCA+AGG + chr4_2:2375817-2375836 None:intergenic 40.0%
CTTGCCAACATCATGTCATT+TGG - chr4_2:2375815-2375834 Msa0583680:intron 40.0%
GCTTCCAAATGACATGATGT+TGG + chr4_2:2375822-2375841 None:intergenic 40.0%
! AGTACCAAGAGTGTATTGCA+AGG + chr4_2:2375844-2375863 None:intergenic 40.0%
TGCAATACACTCTTGGTACT+TGG - chr4_2:2375844-2375863 Msa0583680:CDS 40.0%
AGTATCTCTACTAGAATGGG+AGG + chr4_2:2375901-2375920 None:intergenic 40.0%
GAGATACTTGTCTGTGCAAT+TGG - chr4_2:2375913-2375932 Msa0583680:CDS 40.0%
! TTTCTCAGTGTGCTATTCTC+TGG - chr4_2:2376134-2376153 Msa0583680:five_prime_UTR 40.0%
!! TGGTGTTCTGGTTCTGAGAA+AGG + chr4_2:2372986-2373005 None:intergenic 45.0%
! AACCGCGGTTGTTATTAGAG+CGG + chr4_2:2373034-2373053 None:intergenic 45.0%
GATGGCTAAGAAAACAACCG+CGG + chr4_2:2373049-2373068 None:intergenic 45.0%
!! GCGGTTGTTTTCTTAGCCAT+CGG - chr4_2:2373048-2373067 Msa0583680:three_prime_UTR 45.0%
! ATGAGCGAGTTTGAGGTAAG+TGG + chr4_2:2373121-2373140 None:intergenic 45.0%
TGTTTGCATTGCTGTCACTG+TGG - chr4_2:2373382-2373401 Msa0583680:intron 45.0%
CCACGGATGAAGATAACCAA+AGG + chr4_2:2373411-2373430 None:intergenic 45.0%
CCTTTGGTTATCTTCATCCG+TGG - chr4_2:2373408-2373427 Msa0583680:intron 45.0%
CTGCGAAGCTTACTTTAAGC+TGG - chr4_2:2373715-2373734 Msa0583680:intron 45.0%
AGACGGTTTGAGAGAGCTTA+CGG + chr4_2:2373928-2373947 None:intergenic 45.0%
CGGTATAAGTGCTCATGAGA+CGG + chr4_2:2373945-2373964 None:intergenic 45.0%
CGTCTCATGAGCACTTATAC+CGG - chr4_2:2373943-2373962 Msa0583680:intron 45.0%
TCATGAGCACTTATACCGGT+CGG - chr4_2:2373947-2373966 Msa0583680:intron 45.0%
ACAGGAAATGAGGACAGAAG+AGG + chr4_2:2374549-2374568 None:intergenic 45.0%
TGTTGCATCAGTGCAAGTTC+GGG + chr4_2:2374594-2374613 None:intergenic 45.0%
CTGTTGCATCAGTGCAAGTT+CGG + chr4_2:2374595-2374614 None:intergenic 45.0%
CTTCGTACCATACAACATCG+CGG + chr4_2:2374862-2374881 None:intergenic 45.0%
!! GTATGGTACGAAGTATTGGG+CGG - chr4_2:2374869-2374888 Msa0583680:intron 45.0%
CGCTTACTTGATTCTCGTGT+TGG + chr4_2:2374902-2374921 None:intergenic 45.0%
GGTAACTGGTAAGACTAGAC+GGG - chr4_2:2374954-2374973 Msa0583680:intron 45.0%
AGACTAGACGGGATAGGATT+AGG - chr4_2:2374965-2374984 Msa0583680:intron 45.0%
GACACCATTAGAGAGAGTTG+GGG - chr4_2:2374991-2375010 Msa0583680:intron 45.0%
AAGCATCATCCCACTAAGTG+GGG + chr4_2:2375261-2375280 None:intergenic 45.0%
CAAGCATCATCCCACTAAGT+GGG + chr4_2:2375262-2375281 None:intergenic 45.0%
GAAGCCTTGCAATACACTCT+TGG - chr4_2:2375837-2375856 Msa0583680:CDS 45.0%
ATACACTCTTGGTACTTGGC+AGG - chr4_2:2375848-2375867 Msa0583680:CDS 45.0%
ATGTTTGCACAGCAAGACAC+CGG + chr4_2:2375987-2376006 None:intergenic 45.0%
AGCCGCTCTAATAACAACCG+CGG - chr4_2:2373029-2373048 Msa0583680:three_prime_UTR 50.0%
GAGGTAAGTGGAAAGAGTGG+TGG + chr4_2:2373109-2373128 None:intergenic 50.0%
CTCAAACTCGCTCATCTCCT+CGG - chr4_2:2373126-2373145 Msa0583680:exon 50.0%
GATGAAGGTGACCCATAGAG+CGG + chr4_2:2373172-2373191 None:intergenic 50.0%
GCTCTATGGGTCACCTTCAT+CGG - chr4_2:2373171-2373190 Msa0583680:CDS 50.0%
CATGATGATACCGCCGATGA+AGG + chr4_2:2373187-2373206 None:intergenic 50.0%
! GGCTTTTTGAACTTGCCGAC+CGG + chr4_2:2373965-2373984 None:intergenic 50.0%
TTGCATCAGTGCAAGTTCGG+GGG + chr4_2:2374592-2374611 None:intergenic 50.0%
GTTGCATCAGTGCAAGTTCG+GGG + chr4_2:2374593-2374612 None:intergenic 50.0%
AGTCAGACCGCGATGTTGTA+TGG - chr4_2:2374852-2374871 Msa0583680:intron 50.0%
AGGATGTTGCGTTGGATGAG+TGG - chr4_2:2374933-2374952 Msa0583680:intron 50.0%
TGCGTTGGATGAGTGGTAAC+TGG - chr4_2:2374940-2374959 Msa0583680:intron 50.0%
CTGGTAAGACTAGACGGGAT+AGG - chr4_2:2374959-2374978 Msa0583680:intron 50.0%
GCTACCCCAACTCTCTCTAA+TGG + chr4_2:2374998-2375017 None:intergenic 50.0%
ATCATCCCACTAAGTGGGGT+CGG + chr4_2:2375257-2375276 None:intergenic 50.0%
CCAAGCATCATCCCACTAAG+TGG + chr4_2:2375263-2375282 None:intergenic 50.0%
CCACTTAGTGGGATGATGCT+TGG - chr4_2:2375260-2375279 Msa0583680:intron 50.0%
GTATTGGAGAGGAAGTTGGG+TGG - chr4_2:2375708-2375727 Msa0583680:intron 50.0%
AGCAAGACACCGGATACTCT+TGG + chr4_2:2375977-2375996 None:intergenic 50.0%
AACCAGATTCCAGCGCTCGT+GGG + chr4_2:2372876-2372895 None:intergenic 55.0%
AAACCAGATTCCAGCGCTCG+TGG + chr4_2:2372877-2372896 None:intergenic 55.0%
!! ACGAGCGCTGGAATCTGGTT+TGG - chr4_2:2372876-2372895 Msa0583680:three_prime_UTR 55.0%
ACCACCACCACCGTCATCAA+TGG - chr4_2:2373002-2373021 Msa0583680:three_prime_UTR 55.0%
CCACCACCACCGTCATCAAT+GGG - chr4_2:2373003-2373022 Msa0583680:three_prime_UTR 55.0%
!! CTACCCATTGATGACGGTGG+TGG + chr4_2:2373009-2373028 None:intergenic 55.0%
!! GCTCTACCCATTGATGACGG+TGG + chr4_2:2373012-2373031 None:intergenic 55.0%
GCGGCTCTACCCATTGATGA+CGG + chr4_2:2373015-2373034 None:intergenic 55.0%
CGAGGAGATGAGCGAGTTTG+AGG + chr4_2:2373128-2373147 None:intergenic 55.0%
CTCGGCTTCTCCATCGCTTT+CGG - chr4_2:2373144-2373163 Msa0583680:CDS 55.0%
CTATGGGTCACCTTCATCGG+CGG - chr4_2:2373174-2373193 Msa0583680:CDS 55.0%
GCTGTCACTGTGGCTTCCTT+TGG - chr4_2:2373392-2373411 Msa0583680:intron 55.0%
ACTAAGTGGGGTCGGCTACA+TGG + chr4_2:2375249-2375268 None:intergenic 55.0%
ATGTAGCCGACCCCACTTAG+TGG - chr4_2:2375248-2375267 Msa0583680:intron 55.0%
TGTAGCCGACCCCACTTAGT+GGG - chr4_2:2375249-2375268 Msa0583680:intron 55.0%
CAATCCAAACCCACGAGCGC+TGG - chr4_2:2372864-2372883 Msa0583680:three_prime_UTR 60.0%
GATTCCAGCGCTCGTGGGTT+TGG + chr4_2:2372871-2372890 None:intergenic 60.0%
!! AACCCACGAGCGCTGGAATC+TGG - chr4_2:2372871-2372890 Msa0583680:three_prime_UTR 60.0%
!! TGACGGTGGTGGTGGTGTTC+TGG + chr4_2:2372998-2373017 None:intergenic 60.0%
!! CCCATTGATGACGGTGGTGG+TGG + chr4_2:2373006-2373025 None:intergenic 60.0%
CGAAAGCGATGGAGAAGCCG+AGG + chr4_2:2373146-2373165 None:intergenic 60.0%
! TAGAGCGGCGCCGAAAGCGA+TGG + chr4_2:2373157-2373176 None:intergenic 65.0%
TCGCTTTCGGCGCCGCTCTA+TGG - chr4_2:2373157-2373176 Msa0583680:CDS 65.0%
CGCTTTCGGCGCCGCTCTAT+GGG - chr4_2:2373158-2373177 Msa0583680:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 2372806 2376224 2372806 ID=Msa0583680;Name=Msa0583680
chr4_2 mRNA 2372806 2376224 2372806 ID=Msa0583680-mRNA-1;Parent=Msa0583680;Name=Msa0583680-mRNA-1;_AED=0.06;_eAED=0.06;_QI=215|1|1|1|1|1|3|338|208
chr4_2 exon 2372806 2373359 2372806 ID=Msa0583680-mRNA-1:exon:637;Parent=Msa0583680-mRNA-1
chr4_2 exon 2375498 2375717 2375498 ID=Msa0583680-mRNA-1:exon:636;Parent=Msa0583680-mRNA-1
chr4_2 exon 2375819 2376224 2375819 ID=Msa0583680-mRNA-1:exon:635;Parent=Msa0583680-mRNA-1
chr4_2 five_prime_UTR 2376010 2376224 2376010 ID=Msa0583680-mRNA-1:five_prime_utr;Parent=Msa0583680-mRNA-1
chr4_2 CDS 2375819 2376009 2375819 ID=Msa0583680-mRNA-1:cds;Parent=Msa0583680-mRNA-1
chr4_2 CDS 2375498 2375717 2375498 ID=Msa0583680-mRNA-1:cds;Parent=Msa0583680-mRNA-1
chr4_2 CDS 2373144 2373359 2373144 ID=Msa0583680-mRNA-1:cds;Parent=Msa0583680-mRNA-1
chr4_2 three_prime_UTR 2372806 2373143 2372806 ID=Msa0583680-mRNA-1:three_prime_utr;Parent=Msa0583680-mRNA-1
Gene Sequence

>Msa0583680

ATGGGTAGAGCCGCTCTAATAACAACCGCGGTTGTTTTCTTAGCCATCGGAGTAATTTTCTCACCAGAAACTTTCGGATCAAAATCCACCACTCTTTCCACTTACCTCAAACTCGCTCATCTCCTCGGCTTCTCCATCGCTTTCGGCGCCGCTCTATGGGTCACCTTCATCGGCGGTATCATCATGTTCAAGAATTTGCCTAGGCATCAATTTGGTAATCTTCAAAGCAAAATGTTTCCAGCTTATTTTTCAATGGTTGGTGTTTGCATTGCTGTCACTGTGGCTTCCTTTGGTTATCTTCATCCGTGGAAAACTTCTTCCACTAGTGAAAGATACCAACTTGGTTTCTTGATCTCTGCTCTTGCTTTCAATCTTACCAATTTGTTCGTCTTTACACCAATGACAATTGAGATGATGAAACAAAGGCATAAAGTGGAGAGAGAGAATAGTATTGGAGAGGAAGTTGGGTGGTCAAAAAATGTCGAAGTTGCAAAGAAAAATCCAAAGCTTGCAAAGATGAATAAGAAGTTTGGTATGATTCATGGGTTATCTTCCCTTGCCAACATCATGTCATTTGGAAGCCTTGCAATACACTCTTGGTACTTGGCAGGTAAACTTGATCTCTGA
Protein sequence

>Msa0583680

MGRAALITTAVVFLAIGVIFSPETFGSKSTTLSTYLKLAHLLGFSIAFGAALWVTFIGGIIMFKNLPRHQFGNLQSKMFPAYFSMVGVCIAVTVASFGYLHPWKTSSTSERYQLGFLISALAFNLTNLFVFTPMTIEMMKQRHKVERENSIGEEVGWSKNVEVAKKNPKLAKMNKKFGMIHGLSSLANIMSFGSLAIHSWYLAGKLDL*