AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0081950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0081950 MtrunA17_Chr1g0194891 98.558 208 3 0 1 208 1 208 7.54e-152 419
Msa0081950 MtrunA17_Chr4g0014721 29.268 123 87 0 32 154 228 350 4.45e-15 73.2
Msa0081950 MtrunA17_Chr4g0014741 29.268 123 87 0 32 154 228 350 4.50e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0081950 AT3G62580.1 74.648 213 49 1 1 208 1 213 1.20e-115 328
Msa0081950 AT1G72100.1 28.723 188 121 4 25 206 296 476 9.96e-14 69.7
Msa0081950 AT1G22600.1 28.342 187 114 4 41 208 198 383 1.01e-11 63.5

Find 85 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGCTTTCGGCGCCGCTCTA+TGG 0.164177 1_2:-60704771 Msa0081950:CDS
TTATTCATCTTTGCAAGCTT+TGG 0.242253 1_2:+60702170 None:intergenic
GATTCCAGCGCTCGTGGGTT+TGG 0.328551 1_2:+60705060 None:intergenic
CTTGCCAACATCATGTCATT+TGG 0.339373 1_2:-60702116 Msa0081950:CDS
CGCTTTCGGCGCCGCTCTAT+GGG 0.344664 1_2:-60704770 Msa0081950:CDS
AATAGTATTGGAGAGGAAGT+TGG 0.347347 1_2:-60702227 Msa0081950:CDS
AAGAAGTTTGGTATGATTCA+TGG 0.351606 1_2:-60702149 Msa0081950:CDS
CTCGGCTTCTCCATCGCTTT+CGG 0.353954 1_2:-60704784 Msa0081950:CDS
AATTTGCCTAGGCATCAATT+TGG 0.358606 1_2:-60704614 Msa0081950:CDS
GCTGTCACTGTGGCTTCCTT+TGG 0.364294 1_2:-60704536 Msa0081950:CDS
TTTCTCAGTGTGCTATTCTC+TGG 0.370714 1_2:-60701797 Msa0081950:three_prime_UTR
TGACGGTGGTGGTGGTGTTC+TGG 0.374589 1_2:+60704933 None:intergenic
TAATATGGGGTAGCTGTATT+AGG 0.376418 1_2:-60705126 None:intergenic
AACCCACGAGCGCTGGAATC+TGG 0.377154 1_2:-60705057 Msa0081950:five_prime_UTR
GCAAAGATGAATAAGAAGTT+TGG 0.383264 1_2:-60702161 Msa0081950:CDS
ATTATGCTATAAATTATATA+AGG 0.396138 1_2:-60701833 Msa0081950:three_prime_UTR
TGCAATACACTCTTGGTACT+TGG 0.405780 1_2:-60702087 Msa0081950:CDS
TGGTGTAAAGACGAACAAAT+TGG 0.408776 1_2:+60704430 None:intergenic
TCTAGAAACCGAAGTTCATT+TGG 0.421118 1_2:-60705088 Msa0081950:five_prime_UTR
ATAGTATTGGAGAGGAAGTT+GGG 0.421614 1_2:-60702226 Msa0081950:CDS
CGAGGAGATGAGCGAGTTTG+AGG 0.436447 1_2:+60704803 None:intergenic
GACAAGTATCTCTACTAGAA+TGG 0.440265 1_2:+60702029 None:intergenic
GTGGAGAGAGAGAATAGTAT+TGG 0.444526 1_2:-60702239 Msa0081950:CDS
TAATGAGGTAGTTGAATTGA+AGG 0.451077 1_2:+60704970 None:intergenic
GAGATACTTGTCTGTGCAAT+TGG 0.459576 1_2:-60702018 Msa0081950:three_prime_UTR
CAATCCAAACCCACGAGCGC+TGG 0.468537 1_2:-60705064 Msa0081950:five_prime_UTR
ACAAGTATCTCTACTAGAAT+GGG 0.473721 1_2:+60702030 None:intergenic
AGAAGTTTGGTATGATTCAT+GGG 0.477765 1_2:-60702148 Msa0081950:CDS
GAAAATACCTCAATTGTCAT+TGG 0.481213 1_2:+60704410 None:intergenic
ATGAGCGAGTTTGAGGTAAG+TGG 0.481460 1_2:+60704810 None:intergenic
GAAGCCTTGCAATACACTCT+TGG 0.483767 1_2:-60702094 Msa0081950:CDS
GCTCTATGGGTCACCTTCAT+CGG 0.492086 1_2:-60704757 Msa0081950:CDS
ACGAGCGCTGGAATCTGGTT+TGG 0.492118 1_2:-60705052 Msa0081950:five_prime_UTR
ATTGATTTACCAAGAGTATC+CGG 0.495468 1_2:-60701966 Msa0081950:three_prime_UTR
ACCACCACCACCGTCATCAA+TGG 0.497424 1_2:-60704926 Msa0081950:exon
AGATTACCAAATTGATGCCT+AGG 0.499514 1_2:+60704608 None:intergenic
TGGTGTTCTGGTTCTGAGAA+AGG 0.511223 1_2:+60704945 None:intergenic
AATGAGGTAGTTGAATTGAA+GGG 0.512893 1_2:+60704971 None:intergenic
CCACCACCACCGTCATCAAT+GGG 0.515355 1_2:-60704925 Msa0081950:exon
CTCAAACTCGCTCATCTCCT+CGG 0.520112 1_2:-60704802 Msa0081950:CDS
GATGAAACAAAGGCATAAAG+TGG 0.520254 1_2:-60702258 Msa0081950:CDS
CCCATTGATGACGGTGGTGG+TGG 0.521011 1_2:+60704925 None:intergenic
CTTTACACCAATGACAATTG+AGG 0.521289 1_2:-60704417 Msa0081950:intron
ATACACTCTTGGTACTTGGC+AGG 0.533564 1_2:-60702083 Msa0081950:CDS
AGCAAGACACCGGATACTCT+TGG 0.534506 1_2:+60701957 None:intergenic
GAGAGAGAATAGTATTGGAG+AGG 0.541841 1_2:-60702234 Msa0081950:CDS
TTGTGTTTAGGAATTTGCCT+AGG 0.542157 1_2:-60704625 Msa0081950:intron
TGGTGAGAAAATTACTCCGA+TGG 0.542306 1_2:+60704864 None:intergenic
TTAGCATGTGGGGAATAAAA+CGG 0.543297 1_2:-60701743 Msa0081950:three_prime_UTR
AAACCAGATTCCAGCGCTCG+TGG 0.544546 1_2:+60705054 None:intergenic
TAGAGCGGCGCCGAAAGCGA+TGG 0.548120 1_2:+60704774 None:intergenic
TGGATTGACCAAATGAACTT+CGG 0.562008 1_2:+60705080 None:intergenic
CAAATGACATGATGTTGGCA+AGG 0.567231 1_2:+60702117 None:intergenic
CATGATGATACCGCCGATGA+AGG 0.570088 1_2:+60704744 None:intergenic
AAGTTGGTATCTTTCACTAG+TGG 0.574244 1_2:+60704487 None:intergenic
CGAAAGCGATGGAGAAGCCG+AGG 0.581248 1_2:+60704785 None:intergenic
ATGAGGTAGTTGAATTGAAG+GGG 0.581274 1_2:+60704972 None:intergenic
TTTGAGGTAAGTGGAAAGAG+TGG 0.582513 1_2:+60704819 None:intergenic
ATGTACCTTTCTGTAGTCAA+AGG 0.582953 1_2:-60701865 Msa0081950:three_prime_UTR
GAGGTAAGTGGAAAGAGTGG+TGG 0.583033 1_2:+60704822 None:intergenic
TTTGACTACAGAAAGGTACA+TGG 0.584102 1_2:+60701867 None:intergenic
ACTAGTGAAAGATACCAACT+TGG 0.589922 1_2:-60704485 Msa0081950:CDS
CCTTTGGTTATCTTCATCCG+TGG 0.593206 1_2:-60704520 Msa0081950:CDS
GCTTCCAAATGACATGATGT+TGG 0.594501 1_2:+60702112 None:intergenic
CAGAGATCAAGAAACCAAGT+TGG 0.596357 1_2:+60704471 None:intergenic
AAATGACATGATGTTGGCAA+GGG 0.609102 1_2:+60702118 None:intergenic
AACCAGATTCCAGCGCTCGT+GGG 0.615154 1_2:+60705055 None:intergenic
TGATATAGATGATGAAACAA+AGG 0.622671 1_2:-60702268 Msa0081950:intron
AGTATCTCTACTAGAATGGG+AGG 0.623701 1_2:+60702033 None:intergenic
CCACGGATGAAGATAACCAA+AGG 0.626168 1_2:+60704520 None:intergenic
ATGTTTGCACAGCAAGACAC+CGG 0.626366 1_2:+60701947 None:intergenic
CTACCCATTGATGACGGTGG+TGG 0.630995 1_2:+60704922 None:intergenic
GCGGCTCTACCCATTGATGA+CGG 0.643113 1_2:+60704916 None:intergenic
CTATGGGTCACCTTCATCGG+CGG 0.646660 1_2:-60704754 Msa0081950:CDS
GTATACCTTTGACTACAGAA+AGG 0.656498 1_2:+60701860 None:intergenic
AACCGCGGTTGTTATTAGAG+CGG 0.674556 1_2:+60704897 None:intergenic
GATGAAGGTGACCCATAGAG+CGG 0.683702 1_2:+60704759 None:intergenic
GTTCTGAGAAAGGAGTAATG+AGG 0.684686 1_2:+60704955 None:intergenic
AGTACCAAGAGTGTATTGCA+AGG 0.685013 1_2:+60702090 None:intergenic
TGTTTGCATTGCTGTCACTG+TGG 0.686509 1_2:-60704546 Msa0081950:CDS
TGTACTATCAAAGCATCACA+CGG 0.689750 1_2:-60701990 Msa0081950:three_prime_UTR
GCTCTACCCATTGATGACGG+TGG 0.693373 1_2:+60704919 None:intergenic
GTATTGGAGAGGAAGTTGGG+TGG 0.710494 1_2:-60702223 Msa0081950:CDS
AGCCGCTCTAATAACAACCG+CGG 0.732310 1_2:-60704899 Msa0081950:CDS
GATGGCTAAGAAAACAACCG+CGG 0.788667 1_2:+60704882 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATTAAATTAACATAATTA+GGG + chr1_2:60702178-60702197 None:intergenic 10.0%
!! ATTATGCTATAAATTATATA+AGG - chr1_2:60705018-60705037 Msa0081950:five_prime_UTR 10.0%
!! GATTAAATTAACATAATTAG+GGG + chr1_2:60702177-60702196 None:intergenic 15.0%
!! CTGATTAAATTAACATAATT+AGG + chr1_2:60702179-60702198 None:intergenic 15.0%
!!! TATTTTATTCAAGTAAACTA+AGG + chr1_2:60704958-60704977 None:intergenic 15.0%
!!! TTCTTTTCTTTGTCTTTATA+AGG - chr1_2:60702483-60702502 Msa0081950:intron 20.0%
!!! ATGTTTATAGAATTTTGACA+TGG + chr1_2:60703047-60703066 None:intergenic 20.0%
!! AAATGAAAGAATCAAGAAAA+GGG + chr1_2:60703250-60703269 None:intergenic 20.0%
!! AAAATGAAAGAATCAAGAAA+AGG + chr1_2:60703251-60703270 None:intergenic 20.0%
!! ATTACTTTACCAAATATGTA+TGG - chr1_2:60703292-60703311 Msa0081950:intron 20.0%
!! AATATTTCACCATACATATT+TGG + chr1_2:60703304-60703323 None:intergenic 20.0%
!! AATTTGCCTTTAAATTTAAG+CGG + chr1_2:60703743-60703762 None:intergenic 20.0%
!! TGTAAAAGATGGTAAAAAAT+AGG - chr1_2:60703949-60703968 Msa0081950:intron 20.0%
!! AACTATTAGAAAAGATTTAG+AGG - chr1_2:60704091-60704110 Msa0081950:intron 20.0%
!! AATATAGCATAACATGTATA+GGG + chr1_2:60704259-60704278 None:intergenic 20.0%
!! TAATATAGCATAACATGTAT+AGG + chr1_2:60704260-60704279 None:intergenic 20.0%
!! TTGTAACATGATTTATGTTA+GGG + chr1_2:60704374-60704393 None:intergenic 20.0%
!! TCTAATAAAAAATTAGCATG+TGG - chr1_2:60705096-60705115 Msa0081950:five_prime_UTR 20.0%
!! CTAATAAAAAATTAGCATGT+GGG - chr1_2:60705097-60705116 Msa0081950:five_prime_UTR 20.0%
!! TAATAAAAAATTAGCATGTG+GGG - chr1_2:60705098-60705117 Msa0081950:five_prime_UTR 20.0%
! GAAATTGATTAATTGATGGA+TGG + chr1_2:60702155-60702174 None:intergenic 25.0%
! AGAAGAATAATGAGAAAAGA+CGG - chr1_2:60702584-60702603 Msa0081950:intron 25.0%
!!! ACCTATGCATAAGTTTTATT+AGG - chr1_2:60702702-60702721 Msa0081950:intron 25.0%
!!! CCTATGCATAAGTTTTATTA+GGG - chr1_2:60702703-60702722 Msa0081950:intron 25.0%
! GATTATAGATATCTCTACAA+GGG + chr1_2:60702923-60702942 None:intergenic 25.0%
!! CTTGTATTGCATAAGTTTTT+AGG - chr1_2:60703369-60703388 Msa0081950:intron 25.0%
! AAAAAATATACCGATGGTTA+AGG + chr1_2:60703406-60703425 None:intergenic 25.0%
! CAATTCAAAAAATATACCGA+TGG + chr1_2:60703412-60703431 None:intergenic 25.0%
! AACAATAACAACAAATGTTC+AGG - chr1_2:60703555-60703574 Msa0081950:intron 25.0%
! CAAAAAACAAATCGAACAAA+AGG + chr1_2:60703615-60703634 None:intergenic 25.0%
!!! CTTTTGTTCGATTTGTTTTT+TGG - chr1_2:60703613-60703632 Msa0081950:intron 25.0%
! TACTTTCATATTGCATGTAT+AGG + chr1_2:60703720-60703739 None:intergenic 25.0%
!!! AAAGTACCGCTTAAATTTAA+AGG - chr1_2:60703734-60703753 Msa0081950:intron 25.0%
!! TTTACCATCTTTTACACTAT+AGG + chr1_2:60703945-60703964 None:intergenic 25.0%
! TACAATACTCATAACATTGT+CGG - chr1_2:60704283-60704302 Msa0081950:intron 25.0%
! GTTGTAACATGATTTATGTT+AGG + chr1_2:60704375-60704394 None:intergenic 25.0%
! TAAAGTTTGAAAACTTACGT+TGG + chr1_2:60704443-60704462 None:intergenic 25.0%
! TCTATATCAGACAAACAAAA+TGG + chr1_2:60704572-60704591 None:intergenic 25.0%
! TGATATAGATGATGAAACAA+AGG - chr1_2:60704583-60704602 Msa0081950:CDS 25.0%
AATGAGGTAGTTGAATTGAA+GGG + chr1_2:60701883-60701902 None:intergenic 30.0%
TAATGAGGTAGTTGAATTGA+AGG + chr1_2:60701884-60701903 None:intergenic 30.0%
! ATTTTCTCACCAGAAACTTT+CGG - chr1_2:60701998-60702017 Msa0081950:three_prime_UTR 30.0%
AGGGGAAATTGATTAATTGA+TGG + chr1_2:60702159-60702178 None:intergenic 30.0%
!! GTTTCCAGCTTATTTTTCAA+TGG - chr1_2:60702278-60702297 Msa0081950:intron 30.0%
GAAAATACCTCAATTGTCAT+TGG + chr1_2:60702444-60702463 None:intergenic 30.0%
GAAGAATAATGAGAAAAGAC+GGG - chr1_2:60702585-60702604 Msa0081950:intron 30.0%
GTAAGTTATTCATCCAAACA+TGG + chr1_2:60702676-60702695 None:intergenic 30.0%
CCCTAATAAAACTTATGCAT+AGG + chr1_2:60702706-60702725 None:intergenic 30.0%
CGATTATAGATATCTCTACA+AGG + chr1_2:60702924-60702943 None:intergenic 30.0%
!!! GATAAGCTTTTTCAAACACT+GGG - chr1_2:60702943-60702962 Msa0081950:intron 30.0%
! GTAAGCTCAAATAAGCTTTT+TGG - chr1_2:60702981-60703000 Msa0081950:intron 30.0%
TCTTGCAATTGAATCATCTA+AGG - chr1_2:60703078-60703097 Msa0081950:intron 30.0%
CTAACAGGAATCAACATTTA+AGG + chr1_2:60703109-60703128 None:intergenic 30.0%
TGATTCCTGTTAGTAATTAG+AGG - chr1_2:60703116-60703135 Msa0081950:intron 30.0%
GATTCCTGTTAGTAATTAGA+GGG - chr1_2:60703117-60703136 Msa0081950:intron 30.0%
! CTAAATGTTCTCTTTTGTGT+TGG - chr1_2:60703172-60703191 Msa0081950:intron 30.0%
! GTAATGTTTTCCTTAACCAT+CGG - chr1_2:60703393-60703412 Msa0081950:intron 30.0%
ATTTCCTGTTCTATCTTCTT+TGG - chr1_2:60703483-60703502 Msa0081950:intron 30.0%
AAATCAGTCAACACAAAAGT+AGG - chr1_2:60703645-60703664 Msa0081950:intron 30.0%
AATCAGTCAACACAAAAGTA+GGG - chr1_2:60703646-60703665 Msa0081950:intron 30.0%
TATTGCATGTATAGGCAAAA+TGG + chr1_2:60703712-60703731 None:intergenic 30.0%
AAGTAAGCGTAATAGAGATA+AGG - chr1_2:60703836-60703855 Msa0081950:intron 30.0%
AGATGGTAAAAAATAGGCTT+AGG - chr1_2:60703955-60703974 Msa0081950:intron 30.0%
GATGAGAGTCAAATTATAAG+AGG - chr1_2:60704038-60704057 Msa0081950:intron 30.0%
AGTCAAATTATAAGAGGTAG+AGG - chr1_2:60704044-60704063 Msa0081950:intron 30.0%
AGATTTAGAGGTTAATGAGT+TGG - chr1_2:60704103-60704122 Msa0081950:intron 30.0%
! TAATGAGTTGGATCCAAATA+TGG - chr1_2:60704115-60704134 Msa0081950:intron 30.0%
ATAACATGTATAGGGCAAAA+TGG + chr1_2:60704251-60704270 None:intergenic 30.0%
TCATCTTGCAAACATAAGTT+TGG + chr1_2:60704412-60704431 None:intergenic 30.0%
TTAACCAGAGATATGATTCA+AGG + chr1_2:60704477-60704496 None:intergenic 30.0%
TTATTCATCTTTGCAAGCTT+TGG + chr1_2:60704684-60704703 None:intergenic 30.0%
GCAAAGATGAATAAGAAGTT+TGG - chr1_2:60704690-60704709 Msa0081950:intron 30.0%
AAGAAGTTTGGTATGATTCA+TGG - chr1_2:60704702-60704721 Msa0081950:intron 30.0%
AGAAGTTTGGTATGATTCAT+GGG - chr1_2:60704703-60704722 Msa0081950:intron 30.0%
ACAAGTATCTCTACTAGAAT+GGG + chr1_2:60704824-60704843 None:intergenic 30.0%
!! ATTGATTTACCAAGAGTATC+CGG - chr1_2:60704885-60704904 Msa0081950:CDS 30.0%
TCTAGAAACCGAAGTTCATT+TGG - chr1_2:60701763-60701782 Msa0081950:three_prime_UTR 35.0%
TGGATTGACCAAATGAACTT+CGG + chr1_2:60701774-60701793 None:intergenic 35.0%
ATGAGGTAGTTGAATTGAAG+GGG + chr1_2:60701882-60701901 None:intergenic 35.0%
!! GATTTTGATCCGAAAGTTTC+TGG + chr1_2:60702010-60702029 None:intergenic 35.0%
ACGCTGAATTTCTTGTGTTT+AGG - chr1_2:60702214-60702233 Msa0081950:CDS 35.0%
TTGTGTTTAGGAATTTGCCT+AGG - chr1_2:60702226-60702245 Msa0081950:CDS 35.0%
AATTTGCCTAGGCATCAATT+TGG - chr1_2:60702237-60702256 Msa0081950:CDS 35.0%
AGATTACCAAATTGATGCCT+AGG + chr1_2:60702246-60702265 None:intergenic 35.0%
CCAACCATTGAAAAATAAGC+TGG + chr1_2:60702285-60702304 None:intergenic 35.0%
!! CCAGCTTATTTTTCAATGGT+TGG - chr1_2:60702282-60702301 Msa0081950:intron 35.0%
!! AAGTTGGTATCTTTCACTAG+TGG + chr1_2:60702367-60702386 None:intergenic 35.0%
ACTAGTGAAAGATACCAACT+TGG - chr1_2:60702366-60702385 Msa0081950:intron 35.0%
TGGTGTAAAGACGAACAAAT+TGG + chr1_2:60702424-60702443 None:intergenic 35.0%
CTTTACACCAATGACAATTG+AGG - chr1_2:60702434-60702453 Msa0081950:intron 35.0%
TAGCTTAACACCTTATAGAG+AGG + chr1_2:60702616-60702635 None:intergenic 35.0%
CTTTAAGCTGGAAACTACTT+AGG - chr1_2:60702650-60702669 Msa0081950:intron 35.0%
AAGATATCCTGGAGATCTTA+TGG - chr1_2:60702782-60702801 Msa0081950:intron 35.0%
CTTATTTCCATAAGATCTCC+AGG + chr1_2:60702792-60702811 None:intergenic 35.0%
CTACAAGGGTCTATTTGTAT+TGG + chr1_2:60702909-60702928 None:intergenic 35.0%
!!! CGATAAGCTTTTTCAAACAC+TGG - chr1_2:60702942-60702961 Msa0081950:intron 35.0%
! GGATGGAATTGAGTTTTTGA+AGG - chr1_2:60703138-60703157 Msa0081950:intron 35.0%
AGAAGATAGAACAGGAAATG+AGG + chr1_2:60703482-60703501 None:intergenic 35.0%
TGAACCAAAGAAGATAGAAC+AGG + chr1_2:60703490-60703509 None:intergenic 35.0%
!!! GTTCGATTTGTTTTTTGGTC+TGG - chr1_2:60703618-60703637 Msa0081950:intron 35.0%
TCCACAATGACAAAGGAAAA+AGG + chr1_2:60703678-60703697 None:intergenic 35.0%
TGAAATGTCCACAATGACAA+AGG + chr1_2:60703685-60703704 None:intergenic 35.0%
TGTTGTATGGTACGAAGTAT+TGG - chr1_2:60703788-60703807 Msa0081950:intron 35.0%
! GTTGTATGGTACGAAGTATT+GGG - chr1_2:60703789-60703808 Msa0081950:intron 35.0%
AGCACCTATAGTGTAAAAGA+TGG - chr1_2:60703938-60703957 Msa0081950:intron 35.0%
TGGTAAAAAATAGGCTTAGG+TGG - chr1_2:60703958-60703977 Msa0081950:intron 35.0%
GGTAAAAAATAGGCTTAGGT+GGG - chr1_2:60703959-60703978 Msa0081950:intron 35.0%
CTTCGTACTACAACATCTAT+CGG + chr1_2:60704005-60704024 None:intergenic 35.0%
GATCCAAATATGGTTTCAGA+TGG - chr1_2:60704125-60704144 Msa0081950:intron 35.0%
! GTTCCATCTGAAACCATATT+TGG + chr1_2:60704131-60704150 None:intergenic 35.0%
TGGTTTCAGATGGAACATTA+TGG - chr1_2:60704135-60704154 Msa0081950:intron 35.0%
CATACCTTGAATCATATCTC+TGG - chr1_2:60704470-60704489 Msa0081950:CDS 35.0%
! GATGAAACAAAGGCATAAAG+TGG - chr1_2:60704593-60704612 Msa0081950:CDS 35.0%
! AATAGTATTGGAGAGGAAGT+TGG - chr1_2:60704624-60704643 Msa0081950:intron 35.0%
! ATAGTATTGGAGAGGAAGTT+GGG - chr1_2:60704625-60704644 Msa0081950:intron 35.0%
AAATGACATGATGTTGGCAA+GGG + chr1_2:60704736-60704755 None:intergenic 35.0%
GACAAGTATCTCTACTAGAA+TGG + chr1_2:60704825-60704844 None:intergenic 35.0%
! TGTACTATCAAAGCATCACA+CGG - chr1_2:60704861-60704880 Msa0081950:CDS 35.0%
! TTTGACTACAGAAAGGTACA+TGG + chr1_2:60704987-60705006 None:intergenic 35.0%
ATGTACCTTTCTGTAGTCAA+AGG - chr1_2:60704986-60705005 Msa0081950:five_prime_UTR 35.0%
GTATACCTTTGACTACAGAA+AGG + chr1_2:60704994-60705013 None:intergenic 35.0%
TTAGCATGTGGGGAATAAAA+CGG - chr1_2:60705108-60705127 Msa0081950:five_prime_UTR 35.0%
!! GTTCTGAGAAAGGAGTAATG+AGG + chr1_2:60701899-60701918 None:intergenic 40.0%
TGGTGAGAAAATTACTCCGA+TGG + chr1_2:60701990-60702009 None:intergenic 40.0%
! TTTGAGGTAAGTGGAAAGAG+TGG + chr1_2:60702035-60702054 None:intergenic 40.0%
! CTAGTGGAAGAAGTTTTCCA+CGG + chr1_2:60702351-60702370 None:intergenic 40.0%
CAGAGATCAAGAAACCAAGT+TGG + chr1_2:60702383-60702402 None:intergenic 40.0%
ACGGGTATAACCTCTCTATA+AGG - chr1_2:60702603-60702622 Msa0081950:intron 40.0%
GAAACTACTTAGGCCATGTT+TGG - chr1_2:60702660-60702679 Msa0081950:intron 40.0%
AGACGTGTCATAAGATATCC+TGG - chr1_2:60702771-60702790 Msa0081950:intron 40.0%
! CAATTGAATCATCTAAGGCC+TGG - chr1_2:60703083-60703102 Msa0081950:intron 40.0%
! AGGAATCAACATTTAAGGCC+AGG + chr1_2:60703104-60703123 None:intergenic 40.0%
CCATCCCTCTAATTACTAAC+AGG + chr1_2:60703124-60703143 None:intergenic 40.0%
CCTGTTAGTAATTAGAGGGA+TGG - chr1_2:60703121-60703140 Msa0081950:intron 40.0%
AGCAAGCATCTTCGTTATTC+TGG + chr1_2:60703351-60703370 None:intergenic 40.0%
! GCCTTTTTCCTTTGTCATTG+TGG - chr1_2:60703674-60703693 Msa0081950:intron 40.0%
TAGAGATAAGGATGTTGCGT+TGG - chr1_2:60703848-60703867 Msa0081950:intron 40.0%
TGGTAACTGGTAAGACTAGA+CGG - chr1_2:60703876-60703895 Msa0081950:intron 40.0%
ATGACACCATTAGAGAGAGT+TGG - chr1_2:60703912-60703931 Msa0081950:intron 40.0%
TGACACCATTAGAGAGAGTT+GGG - chr1_2:60703913-60703932 Msa0081950:intron 40.0%
AAAAATAGGCTTAGGTGGGT+TGG - chr1_2:60703963-60703982 Msa0081950:intron 40.0%
! AAAATAGGCTTAGGTGGGTT+GGG - chr1_2:60703964-60703983 Msa0081950:intron 40.0%
AGTACGAAGAGTAGATCAGA+TGG - chr1_2:60704016-60704035 Msa0081950:intron 40.0%
TAGAAGTGAGCAGCAACTAT+TGG + chr1_2:60704530-60704549 None:intergenic 40.0%
GTGGAGAGAGAGAATAGTAT+TGG - chr1_2:60704612-60704631 Msa0081950:CDS 40.0%
! GAGAGAGAATAGTATTGGAG+AGG - chr1_2:60704617-60704636 Msa0081950:CDS 40.0%
CAAATGACATGATGTTGGCA+AGG + chr1_2:60704737-60704756 None:intergenic 40.0%
CTTGCCAACATCATGTCATT+TGG - chr1_2:60704735-60704754 Msa0081950:intron 40.0%
GCTTCCAAATGACATGATGT+TGG + chr1_2:60704742-60704761 None:intergenic 40.0%
! AGTACCAAGAGTGTATTGCA+AGG + chr1_2:60704764-60704783 None:intergenic 40.0%
TGCAATACACTCTTGGTACT+TGG - chr1_2:60704764-60704783 Msa0081950:CDS 40.0%
AGTATCTCTACTAGAATGGG+AGG + chr1_2:60704821-60704840 None:intergenic 40.0%
GAGATACTTGTCTGTGCAAT+TGG - chr1_2:60704833-60704852 Msa0081950:CDS 40.0%
! TTTCTCAGTGTGCTATTCTC+TGG - chr1_2:60705054-60705073 Msa0081950:five_prime_UTR 40.0%
!! TGGTGTTCTGGTTCTGAGAA+AGG + chr1_2:60701909-60701928 None:intergenic 45.0%
! AACCGCGGTTGTTATTAGAG+CGG + chr1_2:60701957-60701976 None:intergenic 45.0%
GATGGCTAAGAAAACAACCG+CGG + chr1_2:60701972-60701991 None:intergenic 45.0%
!! GCGGTTGTTTTCTTAGCCAT+CGG - chr1_2:60701971-60701990 Msa0081950:three_prime_UTR 45.0%
! ATGAGCGAGTTTGAGGTAAG+TGG + chr1_2:60702044-60702063 None:intergenic 45.0%
TGTTTGCATTGCTGTCACTG+TGG - chr1_2:60702305-60702324 Msa0081950:intron 45.0%
CCACGGATGAAGATAACCAA+AGG + chr1_2:60702334-60702353 None:intergenic 45.0%
CCTTTGGTTATCTTCATCCG+TGG - chr1_2:60702331-60702350 Msa0081950:intron 45.0%
CTGCGAAGCTTACTTTAAGC+TGG - chr1_2:60702638-60702657 Msa0081950:intron 45.0%
AGACGGTTTGAGAGAGCTTA+CGG + chr1_2:60702851-60702870 None:intergenic 45.0%
CGGTATAAGTGCTCATGAGA+CGG + chr1_2:60702868-60702887 None:intergenic 45.0%
CGTCTCATGAGCACTTATAC+CGG - chr1_2:60702866-60702885 Msa0081950:intron 45.0%
TCATGAGCACTTATACCGGT+CGG - chr1_2:60702870-60702889 Msa0081950:intron 45.0%
ACAGGAAATGAGGACAGAAG+AGG + chr1_2:60703472-60703491 None:intergenic 45.0%
TGTTGCATCAGTGCAAGTTC+GGG + chr1_2:60703517-60703536 None:intergenic 45.0%
CTGTTGCATCAGTGCAAGTT+CGG + chr1_2:60703518-60703537 None:intergenic 45.0%
CTTCGTACCATACAACATCG+CGG + chr1_2:60703785-60703804 None:intergenic 45.0%
!! GTATGGTACGAAGTATTGGG+CGG - chr1_2:60703792-60703811 Msa0081950:intron 45.0%
CGCTTACTTGATTCTCGTGT+TGG + chr1_2:60703825-60703844 None:intergenic 45.0%
GGTAACTGGTAAGACTAGAC+GGG - chr1_2:60703877-60703896 Msa0081950:intron 45.0%
AGACTAGACGGGATAGGATT+AGG - chr1_2:60703888-60703907 Msa0081950:intron 45.0%
GACACCATTAGAGAGAGTTG+GGG - chr1_2:60703914-60703933 Msa0081950:intron 45.0%
AAGCATCATCCCACTAAGTG+GGG + chr1_2:60704184-60704203 None:intergenic 45.0%
CAAGCATCATCCCACTAAGT+GGG + chr1_2:60704185-60704204 None:intergenic 45.0%
GAAGCCTTGCAATACACTCT+TGG - chr1_2:60704757-60704776 Msa0081950:CDS 45.0%
ATACACTCTTGGTACTTGGC+AGG - chr1_2:60704768-60704787 Msa0081950:CDS 45.0%
ATGTTTGCACAGCAAGACAC+CGG + chr1_2:60704907-60704926 None:intergenic 45.0%
AGCCGCTCTAATAACAACCG+CGG - chr1_2:60701952-60701971 Msa0081950:three_prime_UTR 50.0%
GAGGTAAGTGGAAAGAGTGG+TGG + chr1_2:60702032-60702051 None:intergenic 50.0%
CTCAAACTCGCTCATCTCCT+CGG - chr1_2:60702049-60702068 Msa0081950:exon 50.0%
GATGAAGGTGACCCATAGAG+CGG + chr1_2:60702095-60702114 None:intergenic 50.0%
GCTCTATGGGTCACCTTCAT+CGG - chr1_2:60702094-60702113 Msa0081950:CDS 50.0%
CATGATGATACCGCCGATGA+AGG + chr1_2:60702110-60702129 None:intergenic 50.0%
! GGCTTTTTGAACTTGCCGAC+CGG + chr1_2:60702888-60702907 None:intergenic 50.0%
TTGCATCAGTGCAAGTTCGG+GGG + chr1_2:60703515-60703534 None:intergenic 50.0%
GTTGCATCAGTGCAAGTTCG+GGG + chr1_2:60703516-60703535 None:intergenic 50.0%
AGTCAGACCGCGATGTTGTA+TGG - chr1_2:60703775-60703794 Msa0081950:intron 50.0%
AGGATGTTGCGTTGGATGAG+TGG - chr1_2:60703856-60703875 Msa0081950:intron 50.0%
TGCGTTGGATGAGTGGTAAC+TGG - chr1_2:60703863-60703882 Msa0081950:intron 50.0%
CTGGTAAGACTAGACGGGAT+AGG - chr1_2:60703882-60703901 Msa0081950:intron 50.0%
GCTACCCCAACTCTCTCTAA+TGG + chr1_2:60703921-60703940 None:intergenic 50.0%
ATCATCCCACTAAGTGGGGT+CGG + chr1_2:60704180-60704199 None:intergenic 50.0%
CCAAGCATCATCCCACTAAG+TGG + chr1_2:60704186-60704205 None:intergenic 50.0%
CCACTTAGTGGGATGATGCT+TGG - chr1_2:60704183-60704202 Msa0081950:intron 50.0%
GTATTGGAGAGGAAGTTGGG+TGG - chr1_2:60704628-60704647 Msa0081950:intron 50.0%
AGCAAGACACCGGATACTCT+TGG + chr1_2:60704897-60704916 None:intergenic 50.0%
AACCAGATTCCAGCGCTCGT+GGG + chr1_2:60701799-60701818 None:intergenic 55.0%
AAACCAGATTCCAGCGCTCG+TGG + chr1_2:60701800-60701819 None:intergenic 55.0%
!! ACGAGCGCTGGAATCTGGTT+TGG - chr1_2:60701799-60701818 Msa0081950:three_prime_UTR 55.0%
ACCACCACCACCGTCATCAA+TGG - chr1_2:60701925-60701944 Msa0081950:three_prime_UTR 55.0%
CCACCACCACCGTCATCAAT+GGG - chr1_2:60701926-60701945 Msa0081950:three_prime_UTR 55.0%
!! CTACCCATTGATGACGGTGG+TGG + chr1_2:60701932-60701951 None:intergenic 55.0%
!! GCTCTACCCATTGATGACGG+TGG + chr1_2:60701935-60701954 None:intergenic 55.0%
GCGGCTCTACCCATTGATGA+CGG + chr1_2:60701938-60701957 None:intergenic 55.0%
CGAGGAGATGAGCGAGTTTG+AGG + chr1_2:60702051-60702070 None:intergenic 55.0%
CTCGGCTTCTCCATCGCTTT+CGG - chr1_2:60702067-60702086 Msa0081950:CDS 55.0%
CTATGGGTCACCTTCATCGG+CGG - chr1_2:60702097-60702116 Msa0081950:CDS 55.0%
GCTGTCACTGTGGCTTCCTT+TGG - chr1_2:60702315-60702334 Msa0081950:intron 55.0%
ACTAAGTGGGGTCGGCTACA+TGG + chr1_2:60704172-60704191 None:intergenic 55.0%
ATGTAGCCGACCCCACTTAG+TGG - chr1_2:60704171-60704190 Msa0081950:intron 55.0%
TGTAGCCGACCCCACTTAGT+GGG - chr1_2:60704172-60704191 Msa0081950:intron 55.0%
CAATCCAAACCCACGAGCGC+TGG - chr1_2:60701787-60701806 Msa0081950:three_prime_UTR 60.0%
GATTCCAGCGCTCGTGGGTT+TGG + chr1_2:60701794-60701813 None:intergenic 60.0%
!! AACCCACGAGCGCTGGAATC+TGG - chr1_2:60701794-60701813 Msa0081950:three_prime_UTR 60.0%
!! TGACGGTGGTGGTGGTGTTC+TGG + chr1_2:60701921-60701940 None:intergenic 60.0%
!! CCCATTGATGACGGTGGTGG+TGG + chr1_2:60701929-60701948 None:intergenic 60.0%
CGAAAGCGATGGAGAAGCCG+AGG + chr1_2:60702069-60702088 None:intergenic 60.0%
! TAGAGCGGCGCCGAAAGCGA+TGG + chr1_2:60702080-60702099 None:intergenic 65.0%
TCGCTTTCGGCGCCGCTCTA+TGG - chr1_2:60702080-60702099 Msa0081950:CDS 65.0%
CGCTTTCGGCGCCGCTCTAT+GGG - chr1_2:60702081-60702100 Msa0081950:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 60701729 60705144 60701729 ID=Msa0081950;Name=Msa0081950
chr1_2 mRNA 60701729 60705144 60701729 ID=Msa0081950-mRNA-1;Parent=Msa0081950;Name=Msa0081950-mRNA-1;_AED=0.06;_eAED=0.06;_QI=215|1|1|1|1|1|3|338|208
chr1_2 exon 60701729 60702282 60701729 ID=Msa0081950-mRNA-1:exon:19861;Parent=Msa0081950-mRNA-1
chr1_2 exon 60704418 60704637 60704418 ID=Msa0081950-mRNA-1:exon:19860;Parent=Msa0081950-mRNA-1
chr1_2 exon 60704739 60705144 60704739 ID=Msa0081950-mRNA-1:exon:19859;Parent=Msa0081950-mRNA-1
chr1_2 five_prime_UTR 60704930 60705144 60704930 ID=Msa0081950-mRNA-1:five_prime_utr;Parent=Msa0081950-mRNA-1
chr1_2 CDS 60704739 60704929 60704739 ID=Msa0081950-mRNA-1:cds;Parent=Msa0081950-mRNA-1
chr1_2 CDS 60704418 60704637 60704418 ID=Msa0081950-mRNA-1:cds;Parent=Msa0081950-mRNA-1
chr1_2 CDS 60702067 60702282 60702067 ID=Msa0081950-mRNA-1:cds;Parent=Msa0081950-mRNA-1
chr1_2 three_prime_UTR 60701729 60702066 60701729 ID=Msa0081950-mRNA-1:three_prime_utr;Parent=Msa0081950-mRNA-1
Gene Sequence

>Msa0081950

ATGGGTAGAGCCGCTCTAATAACAACCGCGGTTGTTTTCTTAGCCATCGGAGTAATTTTCTCACCAGAAACTTTCGGATCAAAATCCACCACTCTTTCCACTTACCTCAAACTCGCTCATCTCCTCGGCTTCTCCATCGCTTTCGGCGCCGCTCTATGGGTCACCTTCATCGGCGGTATCATCATGTTCAAGAATTTGCCTAGGCATCAATTTGGTAATCTTCAAAGCAAAATGTTTCCAGCTTATTTTTCAATGGTTGGTGTTTGCATTGCTGTCACTGTGGCTTCCTTTGGTTATCTTCATCCGTGGAAAACTTCTTCCACTAGTGAAAGATACCAACTTGGTTTCTTGATCTCTGCTCTTGCTTTCAATCTTACCAATTTGTTCGTCTTTACACCAATGACAATTGAGATGATGAAACAAAGGCATAAAGTGGAGAGAGAGAATAGTATTGGAGAGGAAGTTGGGTGGTCAAAAAATGTCGAAGTTGCAAAGAAAAATCCAAAGCTTGCAAAGATGAATAAGAAGTTTGGTATGATTCATGGGTTATCTTCCCTTGCCAACATCATGTCATTTGGAAGCCTTGCAATACACTCTTGGTACTTGGCAGGTAAACTTGATCTCTGA
Protein sequence

>Msa0081950

MGRAALITTAVVFLAIGVIFSPETFGSKSTTLSTYLKLAHLLGFSIAFGAALWVTFIGGIIMFKNLPRHQFGNLQSKMFPAYFSMVGVCIAVTVASFGYLHPWKTSSTSERYQLGFLISALAFNLTNLFVFTPMTIEMMKQRHKVERENSIGEEVGWSKNVEVAKKNPKLAKMNKKFGMIHGLSSLANIMSFGSLAIHSWYLAGKLDL*