AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0086540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0086540 MtrunA17_Chr1g0202541 89.706 136 12 2 1 134 1 136 1.21e-67 200
Msa0086540 MtrunA17_Chr1g0202581 86.567 134 16 1 1 134 1 132 3.46e-62 186
Msa0086540 MtrunA17_Chr1g0204291 86.567 134 16 1 1 134 1 132 1.85e-61 184
Msa0086540 MtrunA17_Chr1g0202561 77.857 140 24 5 1 134 1 139 1.22e-51 160
Msa0086540 MtrunA17_Chr6g0469571 58.209 134 44 3 1 133 1 123 3.15e-35 118
Msa0086540 MtrunA17_Chr6g0469551 57.463 134 45 3 1 133 1 123 8.98e-35 117
Msa0086540 MtrunA17_Chr6g0469591 57.778 135 43 4 1 133 1 123 3.67e-33 112
Msa0086540 MtrunA17_Chr6g0469681 54.815 135 50 4 1 133 1 126 1.24e-32 111
Msa0086540 MtrunA17_Chr6g0469651 55.396 139 49 4 1 133 1 132 2.97e-32 110
Msa0086540 MtrunA17_Chr6g0469671 70.000 70 20 1 1 69 1 70 2.72e-29 102
Msa0086540 MtrunA17_Chr8g0387471 47.445 137 57 4 1 133 1 126 3.03e-29 103
Msa0086540 MtrunA17_Chr1g0202501 54.478 134 55 3 1 133 1 129 6.91e-29 102
Msa0086540 MtrunA17_Chr7g0233191 46.341 123 47 4 1 119 1 108 1.68e-26 95.5
Msa0086540 MtrunA17_Chr7g0233201 44.715 123 49 4 1 119 1 108 1.05e-25 93.6
Msa0086540 MtrunA17_Chr7g0233181 59.211 76 30 1 1 75 1 76 1.49e-25 93.2
Msa0086540 MtrunA17_Chr7g0233231 43.902 123 55 2 1 122 1 110 2.09e-25 92.8
Msa0086540 MtrunA17_Chr7g0233251 61.333 75 28 1 1 74 1 75 3.96e-25 92.0
Msa0086540 MtrunA17_Chr7g0233211 59.740 77 30 1 1 76 1 77 8.60e-25 91.3
Msa0086540 MtrunA17_Chr6g0469541 57.895 76 31 1 1 75 1 76 2.42e-24 90.1
Msa0086540 MtrunA17_Chr7g0248671 57.353 68 28 1 1 67 1 68 1.99e-18 76.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0086540 AT5G52740.1 41.667 132 61 4 1 132 1 116 1.08e-19 79.0
Msa0086540 AT5G52760.1 41.007 139 60 6 2 134 4 126 1.88e-19 78.6
Msa0086540 AT1G01490.1 56.338 71 29 2 1 69 1 71 4.66e-17 73.6
Msa0086540 AT1G01490.2 56.338 71 29 2 1 69 1 71 4.66e-17 73.6
Msa0086540 AT1G01490.4 55.072 69 29 2 3 69 30 98 1.95e-15 69.7
Msa0086540 AT5G52750.1 50.769 65 31 1 3 66 5 69 8.65e-14 64.3

Find 40 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGCAGTCTCTGGCCTTTC+AGG 0.156873 1_2:+67259263 Msa0086540:CDS
ATTAATCCTTTCGTGCTCTA+TGG 0.313770 1_2:+67259704 Msa0086540:CDS
TTGATGCAAATTGTAATAGA+AGG 0.314402 1_2:-67258944 None:intergenic
AACTAACCTTCATATTGTTA+TGG 0.351439 1_2:-67258995 None:intergenic
ATTCATCTGATGATTATAAT+AGG 0.369814 1_2:-67259738 None:intergenic
TGTATTAAATTGTGGATTGA+TGG 0.388592 1_2:-67260047 None:intergenic
ACAGAAAATTGTGCTAAAGT+TGG 0.399453 1_2:+67259205 Msa0086540:intron
ACGAAAGGATTAATTATCAT+TGG 0.419156 1_2:-67259695 None:intergenic
AGCTTAGCCACTACAAGTAC+TGG 0.428511 1_2:-67259563 None:intergenic
TATATGATGACACAGCCATT+AGG 0.441354 1_2:-67259803 None:intergenic
TGGGTCGGTATCTCCTACTA+AGG 0.446407 1_2:-67259543 None:intergenic
AGGGAAAGAATGAAGAGCTA+GGG 0.448627 1_2:-67258924 None:intergenic
GACAAGAAAATGACCTTAGT+AGG 0.452020 1_2:+67259530 Msa0086540:CDS
GCGAGACTATGTAAGGGCTT+TGG 0.456868 1_2:+67260022 Msa0086540:three_prime_UTR
AGGATCCTCTTCAACACTTA+CGG 0.467744 1_2:-67259783 None:intergenic
AAGGGAAAGAATGAAGAGCT+AGG 0.471515 1_2:-67258925 None:intergenic
AAAGCTATGAAGGCAGTCTC+TGG 0.474866 1_2:+67259254 Msa0086540:CDS
GAGGCTCGCGAGACTATGTA+AGG 0.474901 1_2:+67260015 Msa0086540:three_prime_UTR
TTCAGTTGGACCAGCTAAAG+TGG 0.482641 1_2:+67259613 Msa0086540:CDS
GGGAAAGAATGAAGAGCTAG+GGG 0.487866 1_2:-67258923 None:intergenic
GCACGGTAGTATGAATTGTA+TGG 0.489699 1_2:-67259761 None:intergenic
ATTGAAGTGGTAATAGGAAG+AGG 0.495528 1_2:-67258899 None:intergenic
TCTACTAATGTATTAAATTG+TGG 0.499922 1_2:-67260055 None:intergenic
TGATGATTATAATAGGTAGT+AGG 0.521368 1_2:-67259731 None:intergenic
CATGCAGAAATACTTTCAGT+TGG 0.545738 1_2:+67259599 Msa0086540:CDS
TACTTGTAGTGGCTAAGCTA+AGG 0.547972 1_2:+67259567 Msa0086540:CDS
CCGTGCCGTAAGTGTTGAAG+AGG 0.551299 1_2:+67259778 Msa0086540:CDS
TGATGCAAATTGTAATAGAA+GGG 0.565633 1_2:-67258943 None:intergenic
TAACCTTCATATTGTTATGG+AGG 0.572622 1_2:-67258992 None:intergenic
TAGCCTCCATAACAATATGA+AGG 0.575947 1_2:+67258989 Msa0086540:exon
TTATGGAGGCTACTGAGCTA+AGG 0.584571 1_2:-67258978 None:intergenic
AGTGTTGAAGAGGATCCTAA+TGG 0.585153 1_2:+67259788 Msa0086540:CDS
GGTGTACCATAGAGCACGAA+AGG 0.612408 1_2:-67259710 None:intergenic
GCTTAGCCACTACAAGTACT+GGG 0.632143 1_2:-67259562 None:intergenic
AGCCACTACAAGTACTGGGT+CGG 0.650008 1_2:-67259558 None:intergenic
CCTCTTCAACACTTACGGCA+CGG 0.651373 1_2:-67259778 None:intergenic
TACCGACCCAGTACTTGTAG+TGG 0.655849 1_2:+67259556 Msa0086540:CDS
AGTGTGTCGATCATAGTATG+AGG 0.659198 1_2:+67259996 Msa0086540:three_prime_UTR
GGAAAGAATGAAGAGCTAGG+GGG 0.682663 1_2:-67258922 None:intergenic
AGGCTCGCGAGACTATGTAA+GGG 0.683484 1_2:+67260016 Msa0086540:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAAAAATAATTATTTTTTG+AGG + chr1_2:67259865-67259884 Msa0086540:three_prime_UTR 10.0%
!!! AGAAAAATAATTATTTTTTG+AGG + chr1_2:67259865-67259884 Msa0086540:three_prime_UTR 10.0%
!!! TTGTATTTTAATAATTTTGC+AGG + chr1_2:67259482-67259501 Msa0086540:intron 15.0%
!!! TGTATTTTAATAATTTTGCA+GGG + chr1_2:67259483-67259502 Msa0086540:intron 15.0%
!!! TTCTTTTTATCATCTTTTTT+AGG - chr1_2:67259653-67259672 None:intergenic 15.0%
!! TAAAAAAGATGATAAAAAGA+AGG + chr1_2:67259652-67259671 Msa0086540:CDS 15.0%
!!! TTGTATTTTAATAATTTTGC+AGG + chr1_2:67259482-67259501 Msa0086540:intron 15.0%
!!! TGTATTTTAATAATTTTGCA+GGG + chr1_2:67259483-67259502 Msa0086540:intron 15.0%
!!! TTCTTTTTATCATCTTTTTT+AGG - chr1_2:67259653-67259672 None:intergenic 15.0%
!! TAAAAAAGATGATAAAAAGA+AGG + chr1_2:67259652-67259671 Msa0086540:CDS 15.0%
!!! AAAAAATCTACTTGTTAGAA+CGG - chr1_2:67259180-67259199 None:intergenic 20.0%
!!! ATTTTTTTTCTTTACCTGAA+AGG - chr1_2:67259280-67259299 None:intergenic 20.0%
!!! GTATTTTAATAATTTTGCAG+GGG + chr1_2:67259484-67259503 Msa0086540:intron 20.0%
!! ATTCATCTGATGATTATAAT+AGG - chr1_2:67259741-67259760 None:intergenic 20.0%
!!! TCATTATATACTTTTTTGAG+AGG + chr1_2:67259902-67259921 Msa0086540:three_prime_UTR 20.0%
!!! CATTATATACTTTTTTGAGA+GGG + chr1_2:67259903-67259922 Msa0086540:three_prime_UTR 20.0%
!! TCTACTAATGTATTAAATTG+TGG - chr1_2:67260058-67260077 None:intergenic 20.0%
!!! AAAAAATCTACTTGTTAGAA+CGG - chr1_2:67259180-67259199 None:intergenic 20.0%
!!! ATTTTTTTTCTTTACCTGAA+AGG - chr1_2:67259280-67259299 None:intergenic 20.0%
!!! GTATTTTAATAATTTTGCAG+GGG + chr1_2:67259484-67259503 Msa0086540:intron 20.0%
!! ATTCATCTGATGATTATAAT+AGG - chr1_2:67259741-67259760 None:intergenic 20.0%
!!! TCATTATATACTTTTTTGAG+AGG + chr1_2:67259902-67259921 Msa0086540:three_prime_UTR 20.0%
!!! CATTATATACTTTTTTGAGA+GGG + chr1_2:67259903-67259922 Msa0086540:three_prime_UTR 20.0%
!! TCTACTAATGTATTAAATTG+TGG - chr1_2:67260058-67260077 None:intergenic 20.0%
! TGATGCAAATTGTAATAGAA+GGG - chr1_2:67258946-67258965 None:intergenic 25.0%
! TTGATGCAAATTGTAATAGA+AGG - chr1_2:67258947-67258966 None:intergenic 25.0%
! AACTAACCTTCATATTGTTA+TGG - chr1_2:67258998-67259017 None:intergenic 25.0%
!!! TAAAAGAGTGGTTTTAATCA+AGG - chr1_2:67259030-67259049 None:intergenic 25.0%
! TCATACTTTAGCTATGATAT+AGG - chr1_2:67259056-67259075 None:intergenic 25.0%
! TATCATAGCTAAAGTATGAT+TGG + chr1_2:67259057-67259076 Msa0086540:intron 25.0%
! TTGAAATTGTGCATAAGAAA+AGG - chr1_2:67259091-67259110 None:intergenic 25.0%
! AATCAAACAAAAAGCTATGA+AGG + chr1_2:67259244-67259263 Msa0086540:CDS 25.0%
! ATCGAACTTAGTCTAATATA+CGG - chr1_2:67259371-67259390 None:intergenic 25.0%
!!! ACTAAGTTCGATATTTTGAT+TGG + chr1_2:67259379-67259398 Msa0086540:intron 25.0%
!!! AATCATTGATTCTACGAATT+TGG - chr1_2:67259427-67259446 None:intergenic 25.0%
! ACGAAAGGATTAATTATCAT+TGG - chr1_2:67259698-67259717 None:intergenic 25.0%
! TGATGATTATAATAGGTAGT+AGG - chr1_2:67259734-67259753 None:intergenic 25.0%
!! TGTATTAAATTGTGGATTGA+TGG - chr1_2:67260050-67260069 None:intergenic 25.0%
! TGATGCAAATTGTAATAGAA+GGG - chr1_2:67258946-67258965 None:intergenic 25.0%
! TTGATGCAAATTGTAATAGA+AGG - chr1_2:67258947-67258966 None:intergenic 25.0%
! AACTAACCTTCATATTGTTA+TGG - chr1_2:67258998-67259017 None:intergenic 25.0%
!!! TAAAAGAGTGGTTTTAATCA+AGG - chr1_2:67259030-67259049 None:intergenic 25.0%
! TCATACTTTAGCTATGATAT+AGG - chr1_2:67259056-67259075 None:intergenic 25.0%
! TATCATAGCTAAAGTATGAT+TGG + chr1_2:67259057-67259076 Msa0086540:intron 25.0%
! TTGAAATTGTGCATAAGAAA+AGG - chr1_2:67259091-67259110 None:intergenic 25.0%
! AATCAAACAAAAAGCTATGA+AGG + chr1_2:67259244-67259263 Msa0086540:CDS 25.0%
! ATCGAACTTAGTCTAATATA+CGG - chr1_2:67259371-67259390 None:intergenic 25.0%
!!! ACTAAGTTCGATATTTTGAT+TGG + chr1_2:67259379-67259398 Msa0086540:intron 25.0%
!!! AATCATTGATTCTACGAATT+TGG - chr1_2:67259427-67259446 None:intergenic 25.0%
! ACGAAAGGATTAATTATCAT+TGG - chr1_2:67259698-67259717 None:intergenic 25.0%
! TGATGATTATAATAGGTAGT+AGG - chr1_2:67259734-67259753 None:intergenic 25.0%
!! TGTATTAAATTGTGGATTGA+TGG - chr1_2:67260050-67260069 None:intergenic 25.0%
TAACCTTCATATTGTTATGG+AGG - chr1_2:67258995-67259014 None:intergenic 30.0%
TGATATAGGAGATAAAAGAG+TGG - chr1_2:67259042-67259061 None:intergenic 30.0%
ACAGAAAATTGTGCTAAAGT+TGG + chr1_2:67259205-67259224 Msa0086540:intron 30.0%
TAACCTTCATATTGTTATGG+AGG - chr1_2:67258995-67259014 None:intergenic 30.0%
TGATATAGGAGATAAAAGAG+TGG - chr1_2:67259042-67259061 None:intergenic 30.0%
ACAGAAAATTGTGCTAAAGT+TGG + chr1_2:67259205-67259224 Msa0086540:intron 30.0%
ATTGAAGTGGTAATAGGAAG+AGG - chr1_2:67258902-67258921 None:intergenic 35.0%
TAGCCTCCATAACAATATGA+AGG + chr1_2:67258989-67259008 Msa0086540:exon 35.0%
! TACCAAAGTGCTTCAAATTC+AGG - chr1_2:67259115-67259134 None:intergenic 35.0%
TTGATTGGACAATTCAGATG+TGG + chr1_2:67259394-67259413 Msa0086540:intron 35.0%
GACAAGAAAATGACCTTAGT+AGG + chr1_2:67259530-67259549 Msa0086540:CDS 35.0%
CATGCAGAAATACTTTCAGT+TGG + chr1_2:67259599-67259618 Msa0086540:CDS 35.0%
! TCTTTCTTTTCCACTTTAGC+TGG - chr1_2:67259626-67259645 None:intergenic 35.0%
ATTAATCCTTTCGTGCTCTA+TGG + chr1_2:67259704-67259723 Msa0086540:CDS 35.0%
TATATGATGACACAGCCATT+AGG - chr1_2:67259806-67259825 None:intergenic 35.0%
ATTGAAGTGGTAATAGGAAG+AGG - chr1_2:67258902-67258921 None:intergenic 35.0%
TAGCCTCCATAACAATATGA+AGG + chr1_2:67258989-67259008 Msa0086540:exon 35.0%
! TACCAAAGTGCTTCAAATTC+AGG - chr1_2:67259115-67259134 None:intergenic 35.0%
TTGATTGGACAATTCAGATG+TGG + chr1_2:67259394-67259413 Msa0086540:intron 35.0%
GACAAGAAAATGACCTTAGT+AGG + chr1_2:67259530-67259549 Msa0086540:CDS 35.0%
CATGCAGAAATACTTTCAGT+TGG + chr1_2:67259599-67259618 Msa0086540:CDS 35.0%
! TCTTTCTTTTCCACTTTAGC+TGG - chr1_2:67259626-67259645 None:intergenic 35.0%
ATTAATCCTTTCGTGCTCTA+TGG + chr1_2:67259704-67259723 Msa0086540:CDS 35.0%
TATATGATGACACAGCCATT+AGG - chr1_2:67259806-67259825 None:intergenic 35.0%
AGGGAAAGAATGAAGAGCTA+GGG - chr1_2:67258927-67258946 None:intergenic 40.0%
AAGGGAAAGAATGAAGAGCT+AGG - chr1_2:67258928-67258947 None:intergenic 40.0%
! AGCCTGAATTTGAAGCACTT+TGG + chr1_2:67259110-67259129 Msa0086540:intron 40.0%
TACTTGTAGTGGCTAAGCTA+AGG + chr1_2:67259567-67259586 Msa0086540:CDS 40.0%
GCACGGTAGTATGAATTGTA+TGG - chr1_2:67259764-67259783 None:intergenic 40.0%
AGGATCCTCTTCAACACTTA+CGG - chr1_2:67259786-67259805 None:intergenic 40.0%
! AGTGTTGAAGAGGATCCTAA+TGG + chr1_2:67259788-67259807 Msa0086540:CDS 40.0%
! AGTGTGTCGATCATAGTATG+AGG + chr1_2:67259996-67260015 Msa0086540:three_prime_UTR 40.0%
AGGGAAAGAATGAAGAGCTA+GGG - chr1_2:67258927-67258946 None:intergenic 40.0%
AAGGGAAAGAATGAAGAGCT+AGG - chr1_2:67258928-67258947 None:intergenic 40.0%
! AGCCTGAATTTGAAGCACTT+TGG + chr1_2:67259110-67259129 Msa0086540:intron 40.0%
TACTTGTAGTGGCTAAGCTA+AGG + chr1_2:67259567-67259586 Msa0086540:CDS 40.0%
GCACGGTAGTATGAATTGTA+TGG - chr1_2:67259764-67259783 None:intergenic 40.0%
AGGATCCTCTTCAACACTTA+CGG - chr1_2:67259786-67259805 None:intergenic 40.0%
! AGTGTTGAAGAGGATCCTAA+TGG + chr1_2:67259788-67259807 Msa0086540:CDS 40.0%
! AGTGTGTCGATCATAGTATG+AGG + chr1_2:67259996-67260015 Msa0086540:three_prime_UTR 40.0%
AGGGGGATTGAAGTGGTAAT+AGG - chr1_2:67258908-67258927 None:intergenic 45.0%
GGAAAGAATGAAGAGCTAGG+GGG - chr1_2:67258925-67258944 None:intergenic 45.0%
GGGAAAGAATGAAGAGCTAG+GGG - chr1_2:67258926-67258945 None:intergenic 45.0%
TTATGGAGGCTACTGAGCTA+AGG - chr1_2:67258981-67259000 None:intergenic 45.0%
AAAGCTATGAAGGCAGTCTC+TGG + chr1_2:67259254-67259273 Msa0086540:CDS 45.0%
GCTTAGCCACTACAAGTACT+GGG - chr1_2:67259565-67259584 None:intergenic 45.0%
AGCTTAGCCACTACAAGTAC+TGG - chr1_2:67259566-67259585 None:intergenic 45.0%
TTCAGTTGGACCAGCTAAAG+TGG + chr1_2:67259613-67259632 Msa0086540:CDS 45.0%
AGGGGGATTGAAGTGGTAAT+AGG - chr1_2:67258908-67258927 None:intergenic 45.0%
GGAAAGAATGAAGAGCTAGG+GGG - chr1_2:67258925-67258944 None:intergenic 45.0%
GGGAAAGAATGAAGAGCTAG+GGG - chr1_2:67258926-67258945 None:intergenic 45.0%
TTATGGAGGCTACTGAGCTA+AGG - chr1_2:67258981-67259000 None:intergenic 45.0%
AAAGCTATGAAGGCAGTCTC+TGG + chr1_2:67259254-67259273 Msa0086540:CDS 45.0%
GCTTAGCCACTACAAGTACT+GGG - chr1_2:67259565-67259584 None:intergenic 45.0%
AGCTTAGCCACTACAAGTAC+TGG - chr1_2:67259566-67259585 None:intergenic 45.0%
TTCAGTTGGACCAGCTAAAG+TGG + chr1_2:67259613-67259632 Msa0086540:CDS 45.0%
AAGAGCTAGGGGGATTGAAG+TGG - chr1_2:67258915-67258934 None:intergenic 50.0%
TGGGTCGGTATCTCCTACTA+AGG - chr1_2:67259546-67259565 None:intergenic 50.0%
TACCGACCCAGTACTTGTAG+TGG + chr1_2:67259556-67259575 Msa0086540:CDS 50.0%
!! AGCCACTACAAGTACTGGGT+CGG - chr1_2:67259561-67259580 None:intergenic 50.0%
GGTGTACCATAGAGCACGAA+AGG - chr1_2:67259713-67259732 None:intergenic 50.0%
CCTCTTCAACACTTACGGCA+CGG - chr1_2:67259781-67259800 None:intergenic 50.0%
AGGCTCGCGAGACTATGTAA+GGG + chr1_2:67260016-67260035 Msa0086540:three_prime_UTR 50.0%
GCGAGACTATGTAAGGGCTT+TGG + chr1_2:67260022-67260041 Msa0086540:three_prime_UTR 50.0%
AAGAGCTAGGGGGATTGAAG+TGG - chr1_2:67258915-67258934 None:intergenic 50.0%
TGGGTCGGTATCTCCTACTA+AGG - chr1_2:67259546-67259565 None:intergenic 50.0%
TACCGACCCAGTACTTGTAG+TGG + chr1_2:67259556-67259575 Msa0086540:CDS 50.0%
!! AGCCACTACAAGTACTGGGT+CGG - chr1_2:67259561-67259580 None:intergenic 50.0%
GGTGTACCATAGAGCACGAA+AGG - chr1_2:67259713-67259732 None:intergenic 50.0%
CCTCTTCAACACTTACGGCA+CGG - chr1_2:67259781-67259800 None:intergenic 50.0%
AGGCTCGCGAGACTATGTAA+GGG + chr1_2:67260016-67260035 Msa0086540:three_prime_UTR 50.0%
GCGAGACTATGTAAGGGCTT+TGG + chr1_2:67260022-67260041 Msa0086540:three_prime_UTR 50.0%
! AAGGCAGTCTCTGGCCTTTC+AGG + chr1_2:67259263-67259282 Msa0086540:CDS 55.0%
!! CCGTGCCGTAAGTGTTGAAG+AGG + chr1_2:67259778-67259797 Msa0086540:CDS 55.0%
GAGGCTCGCGAGACTATGTA+AGG + chr1_2:67260015-67260034 Msa0086540:three_prime_UTR 55.0%
! AAGGCAGTCTCTGGCCTTTC+AGG + chr1_2:67259263-67259282 Msa0086540:CDS 55.0%
!! CCGTGCCGTAAGTGTTGAAG+AGG + chr1_2:67259778-67259797 Msa0086540:CDS 55.0%
GAGGCTCGCGAGACTATGTA+AGG + chr1_2:67260015-67260034 Msa0086540:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 67258852 67260091 67258852 ID=Msa0086540;Name=Msa0086540
chr1_2 mRNA 67258852 67260091 67258852 ID=Msa0086540-mRNA-1;Parent=Msa0086540;Name=Msa0086540-mRNA-1;_AED=0.08;_eAED=0.08;_QI=153|1|1|1|1|1|3|265|134
chr1_2 exon 67258852 67259010 67258852 ID=Msa0086540-mRNA-1:exon:22753;Parent=Msa0086540-mRNA-1
chr1_2 exon 67259209 67259284 67259209 ID=Msa0086540-mRNA-1:exon:22754;Parent=Msa0086540-mRNA-1
chr1_2 exon 67259504 67260091 67259504 ID=Msa0086540-mRNA-1:exon:22755;Parent=Msa0086540-mRNA-1
chr1_2 five_prime_UTR 67258852 67259004 67258852 ID=Msa0086540-mRNA-1:five_prime_utr;Parent=Msa0086540-mRNA-1
chr1_2 CDS 67259005 67259010 67259005 ID=Msa0086540-mRNA-1:cds;Parent=Msa0086540-mRNA-1
chr1_2 CDS 67259209 67259284 67259209 ID=Msa0086540-mRNA-1:cds;Parent=Msa0086540-mRNA-1
chr1_2 CDS 67259504 67259826 67259504 ID=Msa0086540-mRNA-1:cds;Parent=Msa0086540-mRNA-1
chr1_2 three_prime_UTR 67259827 67260091 67259827 ID=Msa0086540-mRNA-1:three_prime_utr;Parent=Msa0086540-mRNA-1
Gene Sequence

>Msa0086540

ATGAAGAAAATTGTGCTAAAGTTGGAGATTCATGAAGACAAAATCAAACAAAAAGCTATGAAGGCAGTCTCTGGCCTTTCAGGGGTTGAGTCAGTTGAAGTAGACAAAGACAAGAAAATGACCTTAGTAGGAGATACCGACCCAGTACTTGTAGTGGCTAAGCTAAGGAAGTTATGTCATGCAGAAATACTTTCAGTTGGACCAGCTAAAGTGGAAAAGAAAGATGAACCTAAAAAAGATGATAAAAAGAAGGAAGATGATAAGAAAGATTCACCAATGATAATTAATCCTTTCGTGCTCTATGGTACACCTACTACCTATTATAATCATCAGATGAATCCATACAATTCATACTACCGTGCCGTAAGTGTTGAAGAGGATCCTAATGGCTGTGTCATCATATAA
Protein sequence

>Msa0086540

MKKIVLKLEIHEDKIKQKAMKAVSGLSGVESVEVDKDKKMTLVGDTDPVLVVAKLRKLCHAEILSVGPAKVEKKDEPKKDDKKKEDDKKDSPMIINPFVLYGTPTTYYNHQMNPYNSYYRAVSVEEDPNGCVII*