Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0092190 | A0A396JZE0 | 99.048 | 105 | 1 | 0 | 1 | 105 | 16 | 120 | 8.63e-59 | 186 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0037200 | Msa0092190 | 0.808548 | 2.934435e-50 | -8.615850e-47 |
| Msa0092190 | Msa0177300 | 0.875619 | 2.670900e-68 | -8.615850e-47 |
| Msa0092190 | Msa0177340 | 0.879306 | 1.387373e-69 | -8.615850e-47 |
| Msa0092190 | Msa1039880 | 0.806511 | 7.939092e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0092190 | MtrunA17_Chr1g0200311 | 99.048 | 105 | 1 | 0 | 1 | 105 | 16 | 120 | 1.66e-62 | 186 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0092190 | AT5G61660.1 | 60.714 | 56 | 21 | 1 | 1 | 56 | 13 | 67 | 2.23e-13 | 62.0 |
Find 59 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCGGGCCACGGTCCAAAT+TGG | 0.259697 | 1_2:-74573526 | Msa0092190:CDS |
| TCTGGCTTTGGTAGAGGTTA+TGG | 0.264164 | 1_2:-74573431 | Msa0092190:CDS |
| AGTGGATGGGGTTACGGTTC+AGG | 0.271763 | 1_2:-74573470 | Msa0092190:CDS |
| AGAGGTTATGGCTATGGTTT+TGG | 0.272187 | 1_2:-74573419 | Msa0092190:CDS |
| GGTTCAGGACATTCTCCTTC+TGG | 0.275589 | 1_2:-74573449 | Msa0092190:CDS |
| TGGGGTTACGGTTCAGGTTC+AGG | 0.287425 | 1_2:-74573464 | Msa0092190:CDS |
| CTCCGAAGGTGACCGATTAT+TGG | 0.290144 | 1_2:+74573277 | None:intergenic |
| TCTGGATCCGGGTCTGGTTA+TGG | 0.298117 | 1_2:-74573383 | Msa0092190:CDS |
| ACCGGGTCTGGGTCTGGATC+CGG | 0.302126 | 1_2:-74573395 | Msa0092190:CDS |
| GCCACTGCTGGCTGCGGCCA+TGG | 0.313937 | 1_2:+74573598 | None:intergenic |
| CCCCAGCTATAGTCCCAATT+TGG | 0.314443 | 1_2:+74573512 | None:intergenic |
| TTTGGAACCGGGTCTGGGTC+TGG | 0.316778 | 1_2:-74573401 | Msa0092190:CDS |
| TCCGACAGGTGCAAGTGGTT+CGG | 0.322949 | 1_2:-74573543 | Msa0092190:CDS |
| GGACATTCTCCTTCTGGCTT+TGG | 0.332030 | 1_2:-74573443 | Msa0092190:CDS |
| CCGACAGGTGCAAGTGGTTC+GGG | 0.361066 | 1_2:-74573542 | Msa0092190:CDS |
| TCGGTCACCTTCGGAGTCTA+AGG | 0.368892 | 1_2:-74573270 | Msa0092190:CDS |
| TCCGGGTCTGGTTATGGATA+TGG | 0.372940 | 1_2:-74573377 | Msa0092190:intron |
| CCGTAACCCCATCCACTTCC+TGG | 0.374994 | 1_2:+74573476 | None:intergenic |
| CCGGGTCTGGGTCTGGATCC+GGG | 0.384946 | 1_2:-74573394 | Msa0092190:CDS |
| TCTGGCAATTCAGGCGGTGG+AGG | 0.390126 | 1_2:-74573317 | Msa0092190:intron |
| TGGGACTATAGCTGGGGATG+GGG | 0.391784 | 1_2:-74573506 | Msa0092190:CDS |
| TAGCTTTCTTCCGCCACTGC+TGG | 0.402255 | 1_2:+74573586 | None:intergenic |
| TCTGGGTCTGGATCCGGGTC+TGG | 0.404022 | 1_2:-74573389 | Msa0092190:CDS |
| TTCGGGCCACGGTCCAAATT+GGG | 0.409857 | 1_2:-74573525 | Msa0092190:CDS |
| TCTCCTTCTGGCTTTGGTAG+AGG | 0.416778 | 1_2:-74573437 | Msa0092190:CDS |
| CCAGGAAGTGGATGGGGTTA+CGG | 0.433066 | 1_2:-74573476 | Msa0092190:CDS |
| TTTGGTAGAGGTTATGGCTA+TGG | 0.433764 | 1_2:-74573425 | Msa0092190:CDS |
| GTCCAAATTGGGACTATAGC+TGG | 0.434287 | 1_2:-74573514 | Msa0092190:CDS |
| GGCGGTGGAGGGCATGGTGG+TGG | 0.444613 | 1_2:-74573305 | Msa0092190:CDS |
| GGGACTATAGCTGGGGATGG+GGG | 0.446113 | 1_2:-74573505 | Msa0092190:CDS |
| GGTCAGCACCAGGAAGTGGA+TGG | 0.451888 | 1_2:-74573484 | Msa0092190:CDS |
| AATTCAGGCGGTGGAGGGCA+TGG | 0.452407 | 1_2:-74573311 | Msa0092190:intron |
| TTTAATTACTCATGGTTGGT+TGG | 0.458788 | 1_2:+74573241 | None:intergenic |
| TCTTCCGCCACTGCTGGCTG+CGG | 0.464894 | 1_2:+74573592 | None:intergenic |
| GGTGCAAGTGGTTCGGGCCA+CGG | 0.474288 | 1_2:-74573536 | Msa0092190:CDS |
| TCCAAATTGGGACTATAGCT+GGG | 0.483029 | 1_2:-74573513 | Msa0092190:CDS |
| AACCAATAATCGGTCACCTT+CGG | 0.485230 | 1_2:-74573279 | Msa0092190:CDS |
| TTAATTTAATTACTCATGGT+TGG | 0.488357 | 1_2:+74573237 | None:intergenic |
| GTCAGCACCAGGAAGTGGAT+GGG | 0.490969 | 1_2:-74573483 | Msa0092190:CDS |
| TTGGGACTATAGCTGGGGAT+GGG | 0.492750 | 1_2:-74573507 | Msa0092190:CDS |
| CTGGCAATTCAGGCGGTGGA+GGG | 0.495997 | 1_2:-74573316 | Msa0092190:intron |
| TCCATGGCCGCAGCCAGCAG+TGG | 0.511233 | 1_2:-74573599 | Msa0092190:CDS |
| ATGGCCGCAGCCAGCAGTGG+CGG | 0.520646 | 1_2:-74573596 | Msa0092190:CDS |
| GTGGTGGTAGAACCAATAAT+CGG | 0.540663 | 1_2:-74573289 | Msa0092190:CDS |
| TCAGCACCAGGAAGTGGATG+GGG | 0.555873 | 1_2:-74573482 | Msa0092190:CDS |
| CGGTCACCTTCGGAGTCTAA+GGG | 0.558343 | 1_2:-74573269 | Msa0092190:CDS |
| CCCGGATCCAGACCCAGACC+CGG | 0.567752 | 1_2:+74573394 | None:intergenic |
| ATTGGGACTATAGCTGGGGA+TGG | 0.573906 | 1_2:-74573508 | Msa0092190:CDS |
| GCAAGCGGTGGATCTCCGAC+AGG | 0.576949 | 1_2:-74573557 | Msa0092190:CDS |
| CCCGAACCACTTGCACCTGT+CGG | 0.592937 | 1_2:+74573542 | None:intergenic |
| TCAGGCGGTGGAGGGCATGG+TGG | 0.594015 | 1_2:-74573308 | Msa0092190:intron |
| AAGCTAGACATGGCAAGCGG+TGG | 0.608347 | 1_2:-74573569 | Msa0092190:CDS |
| GGATCTCCGACAGGTGCAAG+TGG | 0.623717 | 1_2:-74573548 | Msa0092190:CDS |
| ACCATATCCATAACCAGACC+CGG | 0.634754 | 1_2:+74573376 | None:intergenic |
| CCAAATTGGGACTATAGCTG+GGG | 0.653928 | 1_2:-74573512 | Msa0092190:CDS |
| TAACCTCTACCAAAGCCAGA+AGG | 0.659441 | 1_2:+74573434 | None:intergenic |
| TATAGTCCCAATTTGGACCG+TGG | 0.667912 | 1_2:+74573519 | None:intergenic |
| TGGCGGAAGAAAGCTAGACA+TGG | 0.690503 | 1_2:-74573579 | Msa0092190:CDS |
| AGAAAGCTAGACATGGCAAG+CGG | 0.706753 | 1_2:-74573572 | Msa0092190:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TCCAAATTGGGACTATAGCT+GGG | - | chr1_2:74573333-74573352 | Msa0092190:intron | 40.0% | |
| TTTGGTAGAGGTTATGGCTA+TGG | - | chr1_2:74573421-74573440 | Msa0092190:CDS | 40.0% | |
| ! | AGAGGTTATGGCTATGGTTT+TGG | - | chr1_2:74573427-74573446 | Msa0092190:CDS | 40.0% |
| !!! | TATGGCTATGGTTTTGGAAC+CGG | - | chr1_2:74573433-74573452 | Msa0092190:CDS | 40.0% |
| TCTGGTTATGGATATGGTAG+TGG | - | chr1_2:74573475-74573494 | Msa0092190:CDS | 40.0% | |
| GTGGTGGTAGAACCAATAAT+CGG | - | chr1_2:74573557-74573576 | Msa0092190:CDS | 40.0% | |
| AACCAATAATCGGTCACCTT+CGG | - | chr1_2:74573567-74573586 | Msa0092190:CDS | 40.0% | |
| AGAAAGCTAGACATGGCAAG+CGG | - | chr1_2:74573274-74573293 | Msa0092190:CDS | 45.0% | |
| TATAGTCCCAATTTGGACCG+TGG | + | chr1_2:74573330-74573349 | None:intergenic | 45.0% | |
| GTCCAAATTGGGACTATAGC+TGG | - | chr1_2:74573332-74573351 | Msa0092190:intron | 45.0% | |
| CCAAATTGGGACTATAGCTG+GGG | - | chr1_2:74573334-74573353 | Msa0092190:intron | 45.0% | |
| TAACCTCTACCAAAGCCAGA+AGG | + | chr1_2:74573415-74573434 | None:intergenic | 45.0% | |
| !! | TCTGGCTTTGGTAGAGGTTA+TGG | - | chr1_2:74573415-74573434 | Msa0092190:CDS | 45.0% |
| !!! | ATGGCTATGGTTTTGGAACC+GGG | - | chr1_2:74573434-74573453 | Msa0092190:CDS | 45.0% |
| ACCATATCCATAACCAGACC+CGG | + | chr1_2:74573473-74573492 | None:intergenic | 45.0% | |
| !! | GGTTATGGATATGGTAGTGG+TGG | - | chr1_2:74573478-74573497 | Msa0092190:CDS | 45.0% |
| ! | GTTTTACCCTTAGACTCCGA+AGG | + | chr1_2:74573586-74573605 | None:intergenic | 45.0% |
| TGGCGGAAGAAAGCTAGACA+TGG | - | chr1_2:74573267-74573286 | Msa0092190:CDS | 50.0% | |
| CCCCAGCTATAGTCCCAATT+TGG | + | chr1_2:74573337-74573356 | None:intergenic | 50.0% | |
| ATTGGGACTATAGCTGGGGA+TGG | - | chr1_2:74573338-74573357 | Msa0092190:intron | 50.0% | |
| TTGGGACTATAGCTGGGGAT+GGG | - | chr1_2:74573339-74573358 | Msa0092190:intron | 50.0% | |
| GGTTCAGGACATTCTCCTTC+TGG | - | chr1_2:74573397-74573416 | Msa0092190:CDS | 50.0% | |
| !! | GGACATTCTCCTTCTGGCTT+TGG | - | chr1_2:74573403-74573422 | Msa0092190:CDS | 50.0% |
| !! | TCTCCTTCTGGCTTTGGTAG+AGG | - | chr1_2:74573409-74573428 | Msa0092190:CDS | 50.0% |
| !!! | TATGGTTTTGGAACCGGGTC+TGG | - | chr1_2:74573439-74573458 | Msa0092190:CDS | 50.0% |
| !!! | ATGGTTTTGGAACCGGGTCT+GGG | - | chr1_2:74573440-74573459 | Msa0092190:CDS | 50.0% |
| TCCGGGTCTGGTTATGGATA+TGG | - | chr1_2:74573469-74573488 | Msa0092190:CDS | 50.0% | |
| !! | TATGGTAGTGGTGGTGCTCA+TGG | - | chr1_2:74573487-74573506 | Msa0092190:CDS | 50.0% |
| !! | CATGGTGGTGGTTATGGAAG+TGG | - | chr1_2:74573505-74573524 | Msa0092190:CDS | 50.0% |
| GGTGGTTATGGAAGTGGTTC+TGG | - | chr1_2:74573511-74573530 | Msa0092190:CDS | 50.0% | |
| !! | GGAAGTGGTTCTGGCAATTC+AGG | - | chr1_2:74573520-74573539 | Msa0092190:CDS | 50.0% |
| !! | AGTGGTTCTGGCAATTCAGG+CGG | - | chr1_2:74573523-74573542 | Msa0092190:CDS | 50.0% |
| CTCCGAAGGTGACCGATTAT+TGG | + | chr1_2:74573572-74573591 | None:intergenic | 50.0% | |
| TAGCTTTCTTCCGCCACTGC+TGG | + | chr1_2:74573263-74573282 | None:intergenic | 55.0% | |
| AAGCTAGACATGGCAAGCGG+TGG | - | chr1_2:74573277-74573296 | Msa0092190:CDS | 55.0% | |
| TCCGACAGGTGCAAGTGGTT+CGG | - | chr1_2:74573303-74573322 | Msa0092190:CDS | 55.0% | |
| TTCGGGCCACGGTCCAAATT+GGG | - | chr1_2:74573321-74573340 | Msa0092190:intron | 55.0% | |
| TGGGACTATAGCTGGGGATG+GGG | - | chr1_2:74573340-74573359 | Msa0092190:intron | 55.0% | |
| GTCAGCACCAGGAAGTGGAT+GGG | - | chr1_2:74573363-74573382 | Msa0092190:intron | 55.0% | |
| TCAGCACCAGGAAGTGGATG+GGG | - | chr1_2:74573364-74573383 | Msa0092190:intron | 55.0% | |
| CCAGGAAGTGGATGGGGTTA+CGG | - | chr1_2:74573370-74573389 | Msa0092190:intron | 55.0% | |
| AGTGGATGGGGTTACGGTTC+AGG | - | chr1_2:74573376-74573395 | Msa0092190:intron | 55.0% | |
| ! | TGGGGTTACGGTTCAGGTTC+AGG | - | chr1_2:74573382-74573401 | Msa0092190:CDS | 55.0% |
| TCTGGATCCGGGTCTGGTTA+TGG | - | chr1_2:74573463-74573482 | Msa0092190:CDS | 55.0% | |
| !! | GGTGCTCATGGTGGTGGTTA+TGG | - | chr1_2:74573499-74573518 | Msa0092190:CDS | 55.0% |
| TCGGTCACCTTCGGAGTCTA+AGG | - | chr1_2:74573576-74573595 | Msa0092190:CDS | 55.0% | |
| CGGTCACCTTCGGAGTCTAA+GGG | - | chr1_2:74573577-74573596 | Msa0092190:CDS | 55.0% | |
| GGATCTCCGACAGGTGCAAG+TGG | - | chr1_2:74573298-74573317 | Msa0092190:CDS | 60.0% | |
| CCCGAACCACTTGCACCTGT+CGG | + | chr1_2:74573307-74573326 | None:intergenic | 60.0% | |
| CCGACAGGTGCAAGTGGTTC+GGG | - | chr1_2:74573304-74573323 | Msa0092190:CDS | 60.0% | |
| GTTCGGGCCACGGTCCAAAT+TGG | - | chr1_2:74573320-74573339 | Msa0092190:intron | 60.0% | |
| GGGACTATAGCTGGGGATGG+GGG | - | chr1_2:74573341-74573360 | Msa0092190:intron | 60.0% | |
| GGTCAGCACCAGGAAGTGGA+TGG | - | chr1_2:74573362-74573381 | Msa0092190:intron | 60.0% | |
| CCGTAACCCCATCCACTTCC+TGG | + | chr1_2:74573373-74573392 | None:intergenic | 60.0% | |
| ! | TTTGGAACCGGGTCTGGGTC+TGG | - | chr1_2:74573445-74573464 | Msa0092190:CDS | 60.0% |
| ! | GGTAGTGGTGGTGCTCATGG+TGG | - | chr1_2:74573490-74573509 | Msa0092190:CDS | 60.0% |
| ! | AGTGGTGGTGCTCATGGTGG+TGG | - | chr1_2:74573493-74573512 | Msa0092190:CDS | 60.0% |
| ! | GGTTCTGGCAATTCAGGCGG+TGG | - | chr1_2:74573526-74573545 | Msa0092190:CDS | 60.0% |
| TCTGGCAATTCAGGCGGTGG+AGG | - | chr1_2:74573529-74573548 | Msa0092190:CDS | 60.0% | |
| CTGGCAATTCAGGCGGTGGA+GGG | - | chr1_2:74573530-74573549 | Msa0092190:CDS | 60.0% | |
| AATTCAGGCGGTGGAGGGCA+TGG | - | chr1_2:74573535-74573554 | Msa0092190:CDS | 60.0% | |
| ! | TCTTCCGCCACTGCTGGCTG+CGG | + | chr1_2:74573257-74573276 | None:intergenic | 65.0% |
| GCAAGCGGTGGATCTCCGAC+AGG | - | chr1_2:74573289-74573308 | Msa0092190:CDS | 65.0% | |
| ! | GGTGCAAGTGGTTCGGGCCA+CGG | - | chr1_2:74573310-74573329 | Msa0092190:intron | 65.0% |
| TGGGGGTCAGCACCAGGAAG+TGG | - | chr1_2:74573358-74573377 | Msa0092190:intron | 65.0% | |
| ACCGGGTCTGGGTCTGGATC+CGG | - | chr1_2:74573451-74573470 | Msa0092190:CDS | 65.0% | |
| TCTGGGTCTGGATCCGGGTC+TGG | - | chr1_2:74573457-74573476 | Msa0092190:CDS | 65.0% | |
| ATGGCCGCAGCCAGCAGTGG+CGG | - | chr1_2:74573250-74573269 | Msa0092190:CDS | 70.0% | |
| TGGGGATGGGGGTCAGCACC+AGG | - | chr1_2:74573352-74573371 | Msa0092190:intron | 70.0% | |
| CCCGGATCCAGACCCAGACC+CGG | + | chr1_2:74573455-74573474 | None:intergenic | 70.0% | |
| CCGGGTCTGGGTCTGGATCC+GGG | - | chr1_2:74573452-74573471 | Msa0092190:CDS | 70.0% | |
| ! | TCAGGCGGTGGAGGGCATGG+TGG | - | chr1_2:74573538-74573557 | Msa0092190:CDS | 70.0% |
| ! | GGCGGTGGAGGGCATGGTGG+TGG | - | chr1_2:74573541-74573560 | Msa0092190:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 74573250 | 74573618 | 74573250 | ID=Msa0092190;Name=Msa0092190 |
| chr1_2 | mRNA | 74573250 | 74573618 | 74573250 | ID=Msa0092190-mRNA-1;Parent=Msa0092190;Name=Msa0092190-mRNA-1;_AED=0.28;_eAED=0.29;_QI=0|0|0|1|0|0|2|0|105 |
| chr1_2 | exon | 74573378 | 74573618 | 74573378 | ID=Msa0092190-mRNA-1:exon:26284;Parent=Msa0092190-mRNA-1 |
| chr1_2 | exon | 74573250 | 74573326 | 74573250 | ID=Msa0092190-mRNA-1:exon:26283;Parent=Msa0092190-mRNA-1 |
| chr1_2 | CDS | 74573378 | 74573618 | 74573378 | ID=Msa0092190-mRNA-1:cds;Parent=Msa0092190-mRNA-1 |
| chr1_2 | CDS | 74573250 | 74573326 | 74573250 | ID=Msa0092190-mRNA-1:cds;Parent=Msa0092190-mRNA-1 |
| Gene Sequence |
| Protein sequence |
