Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0037200 | Msa0092190 | 0.808548 | 2.934435e-50 | -8.615850e-47 |
| Msa0037200 | Msa0177300 | 0.928301 | 3.505522e-92 | -8.615850e-47 |
| Msa0037200 | Msa0177340 | 0.921857 | 2.086472e-88 | -8.615850e-47 |
| Msa0037200 | Msa0256330 | 0.809104 | 2.231636e-50 | -8.615850e-47 |
| Msa0037200 | Msa0479040 | 0.800462 | 1.425199e-48 | -8.615850e-47 |
| Msa0037200 | Msa0479090 | 0.818390 | 2.010300e-52 | -8.615850e-47 |
| Msa0037200 | Msa0579370 | 0.819863 | 9.288249e-53 | -8.615850e-47 |
| Msa0037200 | Msa0602370 | 0.810253 | 1.263438e-50 | -8.615850e-47 |
| Msa0037200 | Msa0614990 | 0.836270 | 1.032394e-56 | -8.615850e-47 |
| Msa0037200 | Msa0643320 | 0.813031 | 3.144446e-51 | -8.615850e-47 |
| Msa0037200 | Msa0683140 | 0.822666 | 2.096439e-53 | -8.615850e-47 |
| Msa0037200 | Msa0683600 | 0.823346 | 1.455086e-53 | -8.615850e-47 |
| Msa0037200 | Msa1094430 | 0.825341 | 4.940983e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0037200 | MtrunA17_Chr1g0200311 | 98.095 | 105 | 2 | 0 | 1 | 105 | 16 | 120 | 1.44e-61 | 183 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0037200 | AT5G61660.1 | 60.714 | 56 | 21 | 1 | 1 | 56 | 13 | 67 | 2.71e-13 | 61.6 |
Find 59 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCGGGCCACGGTCCAAAT+TGG | 0.259697 | 1_1:+66142575 | Msa0037200:CDS |
| TCTGGCTTTGGTAGAGGTTA+TGG | 0.264164 | 1_1:+66142670 | Msa0037200:CDS |
| AGTGGATGGGGTTACGGTTC+AGG | 0.271763 | 1_1:+66142631 | Msa0037200:CDS |
| AGAGGTTATGGCTATGGTTT+TGG | 0.272187 | 1_1:+66142682 | Msa0037200:CDS |
| GGTTCAGGACATTCTCCTTC+TGG | 0.275589 | 1_1:+66142652 | Msa0037200:CDS |
| GCCACTGCTGGCTGCGGCCA+TGG | 0.278338 | 1_1:-66142503 | None:intergenic |
| TGGGGTTACGGTTCAGGTTC+AGG | 0.287425 | 1_1:+66142637 | Msa0037200:CDS |
| CTCCGAAGGTGACCGATTAT+TGG | 0.290144 | 1_1:-66142824 | None:intergenic |
| TCTGGATCCGGGTCTGGTTA+TGG | 0.298117 | 1_1:+66142718 | Msa0037200:CDS |
| ACCGGGTCTGGGTCTGGATC+CGG | 0.302126 | 1_1:+66142706 | Msa0037200:CDS |
| CCCCAGCTATAGTCCCAATT+TGG | 0.314443 | 1_1:-66142589 | None:intergenic |
| TTTGGAACCGGGTCTGGGTC+TGG | 0.316778 | 1_1:+66142700 | Msa0037200:CDS |
| TCCGACAGGTGCAAGTGGTT+CGG | 0.329780 | 1_1:+66142558 | Msa0037200:CDS |
| GGACATTCTCCTTCTGGCTT+TGG | 0.332030 | 1_1:+66142658 | Msa0037200:CDS |
| CCGACAGGTGCAAGTGGTTC+GGG | 0.360896 | 1_1:+66142559 | Msa0037200:CDS |
| TCGGTCACCTTCGGAGTCTA+AGG | 0.368892 | 1_1:+66142831 | Msa0037200:CDS |
| TCTGGCAATTCAGGCGGTGG+TGG | 0.371357 | 1_1:+66142784 | Msa0037200:intron |
| GTCTGCACCAGGAAGTGGAT+GGG | 0.371552 | 1_1:+66142618 | Msa0037200:CDS |
| TCCGGGTCTGGTTATGGATA+TGG | 0.372940 | 1_1:+66142724 | Msa0037200:CDS |
| CCGTAACCCCATCCACTTCC+TGG | 0.374994 | 1_1:-66142625 | None:intergenic |
| GGTCTGCACCAGGAAGTGGA+TGG | 0.384555 | 1_1:+66142617 | Msa0037200:CDS |
| CCGGGTCTGGGTCTGGATCC+GGG | 0.384946 | 1_1:+66142707 | Msa0037200:CDS |
| TGGGACTATAGCTGGGGATG+GGG | 0.391784 | 1_1:+66142595 | Msa0037200:CDS |
| TAGCTTTCTTCCGCCACTGC+TGG | 0.402255 | 1_1:-66142515 | None:intergenic |
| TCTGGGTCTGGATCCGGGTC+TGG | 0.404022 | 1_1:+66142712 | Msa0037200:CDS |
| TTCGGGCCACGGTCCAAATT+GGG | 0.409857 | 1_1:+66142576 | Msa0037200:CDS |
| TCTCCTTCTGGCTTTGGTAG+AGG | 0.416778 | 1_1:+66142664 | Msa0037200:CDS |
| CCAGGAAGTGGATGGGGTTA+CGG | 0.422757 | 1_1:+66142625 | Msa0037200:CDS |
| TTTGGTAGAGGTTATGGCTA+TGG | 0.433764 | 1_1:+66142676 | Msa0037200:CDS |
| GTCCAAATTGGGACTATAGC+TGG | 0.434287 | 1_1:+66142587 | Msa0037200:CDS |
| GGGACTATAGCTGGGGATGG+GGG | 0.436504 | 1_1:+66142596 | Msa0037200:CDS |
| GGCGGTGGTGGGCATGGTGG+TGG | 0.439153 | 1_1:+66142796 | Msa0037200:intron |
| AATTCAGGCGGTGGTGGGCA+TGG | 0.447135 | 1_1:+66142790 | Msa0037200:intron |
| TTTAATTACTCATGGTTGGT+TGG | 0.458788 | 1_1:-66142860 | None:intergenic |
| TCTTCCGCCACTGCTGGCTG+CGG | 0.464894 | 1_1:-66142509 | None:intergenic |
| CTGGCAATTCAGGCGGTGGT+GGG | 0.467115 | 1_1:+66142785 | Msa0037200:intron |
| GGTGCAAGTGGTTCGGGCCA+CGG | 0.474288 | 1_1:+66142565 | Msa0037200:CDS |
| TCCAAATTGGGACTATAGCT+GGG | 0.483029 | 1_1:+66142588 | Msa0037200:CDS |
| AACCAATAATCGGTCACCTT+CGG | 0.485230 | 1_1:+66142822 | Msa0037200:CDS |
| TTAATTTAATTACTCATGGT+TGG | 0.488357 | 1_1:-66142864 | None:intergenic |
| TTGGGACTATAGCTGGGGAT+GGG | 0.492750 | 1_1:+66142594 | Msa0037200:CDS |
| ATGGCCGCAGCCAGCAGTGG+CGG | 0.522666 | 1_1:+66142505 | Msa0037200:CDS |
| GCAAGCGGTGGTTCTCCGAC+AGG | 0.530173 | 1_1:+66142544 | Msa0037200:CDS |
| GTGGTGGTAGAACCAATAAT+CGG | 0.540663 | 1_1:+66142812 | Msa0037200:CDS |
| TCTGCACCAGGAAGTGGATG+GGG | 0.554469 | 1_1:+66142619 | Msa0037200:CDS |
| CGGTCACCTTCGGAGTCTAA+GGG | 0.558343 | 1_1:+66142832 | Msa0037200:CDS |
| CCCGGATCCAGACCCAGACC+CGG | 0.567752 | 1_1:-66142707 | None:intergenic |
| ATTGGGACTATAGCTGGGGA+TGG | 0.573906 | 1_1:+66142593 | Msa0037200:CDS |
| TCAGGCGGTGGTGGGCATGG+TGG | 0.587192 | 1_1:+66142793 | Msa0037200:intron |
| CCCGAACCACTTGCACCTGT+CGG | 0.592937 | 1_1:-66142559 | None:intergenic |
| TCCATGGCCGCAGCCAGCAG+TGG | 0.594355 | 1_1:+66142502 | None:intergenic |
| AAGCTAGACACGGCAAGCGG+TGG | 0.607144 | 1_1:+66142532 | Msa0037200:CDS |
| GGTTCTCCGACAGGTGCAAG+TGG | 0.627044 | 1_1:+66142553 | Msa0037200:CDS |
| ACCATATCCATAACCAGACC+CGG | 0.634754 | 1_1:-66142725 | None:intergenic |
| CCAAATTGGGACTATAGCTG+GGG | 0.653928 | 1_1:+66142589 | Msa0037200:CDS |
| TAACCTCTACCAAAGCCAGA+AGG | 0.659441 | 1_1:-66142667 | None:intergenic |
| TATAGTCCCAATTTGGACCG+TGG | 0.667912 | 1_1:-66142582 | None:intergenic |
| AGAAAGCTAGACACGGCAAG+CGG | 0.701647 | 1_1:+66142529 | Msa0037200:CDS |
| TGGCGGAAGAAAGCTAGACA+CGG | 0.719665 | 1_1:+66142522 | Msa0037200:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TCCAAATTGGGACTATAGCT+GGG | + | chr1_1:66142588-66142607 | Msa0037200:CDS | 40.0% | |
| TTTGGTAGAGGTTATGGCTA+TGG | + | chr1_1:66142676-66142695 | Msa0037200:CDS | 40.0% | |
| ! | AGAGGTTATGGCTATGGTTT+TGG | + | chr1_1:66142682-66142701 | Msa0037200:CDS | 40.0% |
| !!! | TATGGCTATGGTTTTGGAAC+CGG | + | chr1_1:66142688-66142707 | Msa0037200:CDS | 40.0% |
| TCTGGTTATGGATATGGTAG+TGG | + | chr1_1:66142730-66142749 | Msa0037200:intron | 40.0% | |
| GTGGTGGTAGAACCAATAAT+CGG | + | chr1_1:66142812-66142831 | Msa0037200:CDS | 40.0% | |
| AACCAATAATCGGTCACCTT+CGG | + | chr1_1:66142822-66142841 | Msa0037200:CDS | 40.0% | |
| TATAGTCCCAATTTGGACCG+TGG | - | chr1_1:66142585-66142604 | None:intergenic | 45.0% | |
| GTCCAAATTGGGACTATAGC+TGG | + | chr1_1:66142587-66142606 | Msa0037200:CDS | 45.0% | |
| CCAAATTGGGACTATAGCTG+GGG | + | chr1_1:66142589-66142608 | Msa0037200:CDS | 45.0% | |
| TAACCTCTACCAAAGCCAGA+AGG | - | chr1_1:66142670-66142689 | None:intergenic | 45.0% | |
| !! | TCTGGCTTTGGTAGAGGTTA+TGG | + | chr1_1:66142670-66142689 | Msa0037200:CDS | 45.0% |
| !!! | ATGGCTATGGTTTTGGAACC+GGG | + | chr1_1:66142689-66142708 | Msa0037200:CDS | 45.0% |
| ACCATATCCATAACCAGACC+CGG | - | chr1_1:66142728-66142747 | None:intergenic | 45.0% | |
| !! | GGTTATGGATATGGTAGTGG+TGG | + | chr1_1:66142733-66142752 | Msa0037200:intron | 45.0% |
| ! | GTTTTACCCTTAGACTCCGA+AGG | - | chr1_1:66142841-66142860 | None:intergenic | 45.0% |
| TGGCGGAAGAAAGCTAGACA+CGG | + | chr1_1:66142522-66142541 | Msa0037200:CDS | 50.0% | |
| AGAAAGCTAGACACGGCAAG+CGG | + | chr1_1:66142529-66142548 | Msa0037200:CDS | 50.0% | |
| CCCCAGCTATAGTCCCAATT+TGG | - | chr1_1:66142592-66142611 | None:intergenic | 50.0% | |
| ATTGGGACTATAGCTGGGGA+TGG | + | chr1_1:66142593-66142612 | Msa0037200:CDS | 50.0% | |
| TTGGGACTATAGCTGGGGAT+GGG | + | chr1_1:66142594-66142613 | Msa0037200:CDS | 50.0% | |
| GGTTCAGGACATTCTCCTTC+TGG | + | chr1_1:66142652-66142671 | Msa0037200:CDS | 50.0% | |
| !! | GGACATTCTCCTTCTGGCTT+TGG | + | chr1_1:66142658-66142677 | Msa0037200:CDS | 50.0% |
| !! | TCTCCTTCTGGCTTTGGTAG+AGG | + | chr1_1:66142664-66142683 | Msa0037200:CDS | 50.0% |
| !!! | TATGGTTTTGGAACCGGGTC+TGG | + | chr1_1:66142694-66142713 | Msa0037200:CDS | 50.0% |
| !!! | ATGGTTTTGGAACCGGGTCT+GGG | + | chr1_1:66142695-66142714 | Msa0037200:CDS | 50.0% |
| TCCGGGTCTGGTTATGGATA+TGG | + | chr1_1:66142724-66142743 | Msa0037200:CDS | 50.0% | |
| !! | TATGGTAGTGGTGGTGCTCA+TGG | + | chr1_1:66142742-66142761 | Msa0037200:intron | 50.0% |
| !! | CATGGTGGTGGTTATGGAAG+TGG | + | chr1_1:66142760-66142779 | Msa0037200:intron | 50.0% |
| GGTGGTTATGGAAGTGGTTC+TGG | + | chr1_1:66142766-66142785 | Msa0037200:intron | 50.0% | |
| !! | GGAAGTGGTTCTGGCAATTC+AGG | + | chr1_1:66142775-66142794 | Msa0037200:intron | 50.0% |
| !! | AGTGGTTCTGGCAATTCAGG+CGG | + | chr1_1:66142778-66142797 | Msa0037200:intron | 50.0% |
| CTCCGAAGGTGACCGATTAT+TGG | - | chr1_1:66142827-66142846 | None:intergenic | 50.0% | |
| TAGCTTTCTTCCGCCACTGC+TGG | - | chr1_1:66142518-66142537 | None:intergenic | 55.0% | |
| TCCGACAGGTGCAAGTGGTT+CGG | + | chr1_1:66142558-66142577 | Msa0037200:CDS | 55.0% | |
| TTCGGGCCACGGTCCAAATT+GGG | + | chr1_1:66142576-66142595 | Msa0037200:CDS | 55.0% | |
| TGGGACTATAGCTGGGGATG+GGG | + | chr1_1:66142595-66142614 | Msa0037200:CDS | 55.0% | |
| GTCTGCACCAGGAAGTGGAT+GGG | + | chr1_1:66142618-66142637 | Msa0037200:CDS | 55.0% | |
| TCTGCACCAGGAAGTGGATG+GGG | + | chr1_1:66142619-66142638 | Msa0037200:CDS | 55.0% | |
| CCAGGAAGTGGATGGGGTTA+CGG | + | chr1_1:66142625-66142644 | Msa0037200:CDS | 55.0% | |
| AGTGGATGGGGTTACGGTTC+AGG | + | chr1_1:66142631-66142650 | Msa0037200:CDS | 55.0% | |
| ! | TGGGGTTACGGTTCAGGTTC+AGG | + | chr1_1:66142637-66142656 | Msa0037200:CDS | 55.0% |
| TCTGGATCCGGGTCTGGTTA+TGG | + | chr1_1:66142718-66142737 | Msa0037200:CDS | 55.0% | |
| !! | GGTGCTCATGGTGGTGGTTA+TGG | + | chr1_1:66142754-66142773 | Msa0037200:intron | 55.0% |
| TCGGTCACCTTCGGAGTCTA+AGG | + | chr1_1:66142831-66142850 | Msa0037200:CDS | 55.0% | |
| CGGTCACCTTCGGAGTCTAA+GGG | + | chr1_1:66142832-66142851 | Msa0037200:CDS | 55.0% | |
| AAGCTAGACACGGCAAGCGG+TGG | + | chr1_1:66142532-66142551 | Msa0037200:CDS | 60.0% | |
| GGTTCTCCGACAGGTGCAAG+TGG | + | chr1_1:66142553-66142572 | Msa0037200:CDS | 60.0% | |
| CCCGAACCACTTGCACCTGT+CGG | - | chr1_1:66142562-66142581 | None:intergenic | 60.0% | |
| CCGACAGGTGCAAGTGGTTC+GGG | + | chr1_1:66142559-66142578 | Msa0037200:CDS | 60.0% | |
| GTTCGGGCCACGGTCCAAAT+TGG | + | chr1_1:66142575-66142594 | Msa0037200:CDS | 60.0% | |
| GGGACTATAGCTGGGGATGG+GGG | + | chr1_1:66142596-66142615 | Msa0037200:CDS | 60.0% | |
| GGTCTGCACCAGGAAGTGGA+TGG | + | chr1_1:66142617-66142636 | Msa0037200:CDS | 60.0% | |
| CCGTAACCCCATCCACTTCC+TGG | - | chr1_1:66142628-66142647 | None:intergenic | 60.0% | |
| ! | TTTGGAACCGGGTCTGGGTC+TGG | + | chr1_1:66142700-66142719 | Msa0037200:CDS | 60.0% |
| ! | GGTAGTGGTGGTGCTCATGG+TGG | + | chr1_1:66142745-66142764 | Msa0037200:intron | 60.0% |
| ! | AGTGGTGGTGCTCATGGTGG+TGG | + | chr1_1:66142748-66142767 | Msa0037200:intron | 60.0% |
| ! | GGTTCTGGCAATTCAGGCGG+TGG | + | chr1_1:66142781-66142800 | Msa0037200:intron | 60.0% |
| TCTGGCAATTCAGGCGGTGG+TGG | + | chr1_1:66142784-66142803 | Msa0037200:intron | 60.0% | |
| CTGGCAATTCAGGCGGTGGT+GGG | + | chr1_1:66142785-66142804 | Msa0037200:intron | 60.0% | |
| !! | AATTCAGGCGGTGGTGGGCA+TGG | + | chr1_1:66142790-66142809 | Msa0037200:intron | 60.0% |
| ! | TCTTCCGCCACTGCTGGCTG+CGG | - | chr1_1:66142512-66142531 | None:intergenic | 65.0% |
| GCAAGCGGTGGTTCTCCGAC+AGG | + | chr1_1:66142544-66142563 | Msa0037200:CDS | 65.0% | |
| ! | GGTGCAAGTGGTTCGGGCCA+CGG | + | chr1_1:66142565-66142584 | Msa0037200:CDS | 65.0% |
| TGGGGGTCTGCACCAGGAAG+TGG | + | chr1_1:66142613-66142632 | Msa0037200:CDS | 65.0% | |
| ACCGGGTCTGGGTCTGGATC+CGG | + | chr1_1:66142706-66142725 | Msa0037200:CDS | 65.0% | |
| TCTGGGTCTGGATCCGGGTC+TGG | + | chr1_1:66142712-66142731 | Msa0037200:CDS | 65.0% | |
| ATGGCCGCAGCCAGCAGTGG+CGG | + | chr1_1:66142505-66142524 | Msa0037200:CDS | 70.0% | |
| TGGGGATGGGGGTCTGCACC+AGG | + | chr1_1:66142607-66142626 | Msa0037200:CDS | 70.0% | |
| CCCGGATCCAGACCCAGACC+CGG | - | chr1_1:66142710-66142729 | None:intergenic | 70.0% | |
| CCGGGTCTGGGTCTGGATCC+GGG | + | chr1_1:66142707-66142726 | Msa0037200:CDS | 70.0% | |
| !! | TCAGGCGGTGGTGGGCATGG+TGG | + | chr1_1:66142793-66142812 | Msa0037200:intron | 70.0% |
| !! | GGCGGTGGTGGGCATGGTGG+TGG | + | chr1_1:66142796-66142815 | Msa0037200:intron | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 66142505 | 66142873 | 66142505 | ID=Msa0037200;Name=Msa0037200 |
| chr1_1 | mRNA | 66142505 | 66142873 | 66142505 | ID=Msa0037200-mRNA-1;Parent=Msa0037200;Name=Msa0037200-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|0|0|1|0|0|2|0|105 |
| chr1_1 | exon | 66142505 | 66142745 | 66142505 | ID=Msa0037200-mRNA-1:exon:21367;Parent=Msa0037200-mRNA-1 |
| chr1_1 | exon | 66142797 | 66142873 | 66142797 | ID=Msa0037200-mRNA-1:exon:21368;Parent=Msa0037200-mRNA-1 |
| chr1_1 | CDS | 66142505 | 66142745 | 66142505 | ID=Msa0037200-mRNA-1:cds;Parent=Msa0037200-mRNA-1 |
| chr1_1 | CDS | 66142797 | 66142873 | 66142797 | ID=Msa0037200-mRNA-1:cds;Parent=Msa0037200-mRNA-1 |
| Gene Sequence |
| Protein sequence |
