AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0094320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0094320 MtrunA17_Chr4g0076131 96.552 116 4 0 1 116 1 116 3.04e-84 241
Msa0094320 MtrunA17_Chr5g0447651 55.932 118 50 1 1 116 1 118 6.46e-49 152
Msa0094320 MtrunA17_Chr4g0076141 72.165 97 27 0 19 115 4 100 7.08e-49 151
Msa0094320 MtrunA17_Chr8g0378621 50.588 85 42 0 28 112 364 448 1.02e-25 99.8
Msa0094320 MtrunA17_Chr3g0121271 45.882 85 46 0 28 112 362 446 1.45e-24 96.7
Msa0094320 MtrunA17_Chr4g0075031 51.765 85 41 0 28 112 382 466 2.45e-24 96.3
Msa0094320 MtrunA17_Chr4g0040911 47.059 85 45 0 28 112 37 121 3.12e-23 88.6
Msa0094320 MtrunA17_Chr1g0213651 44.828 87 47 1 28 114 276 361 2.20e-22 89.7
Msa0094320 MtrunA17_Chr8g0378361 38.095 105 64 1 8 112 10 113 3.42e-22 86.3
Msa0094320 MtrunA17_Chr3g0120401 44.444 90 49 1 24 113 153 241 3.46e-22 87.4
Msa0094320 MtrunA17_Chr1g0177171 44.706 85 47 0 28 112 406 490 1.76e-21 87.8
Msa0094320 MtrunA17_Chr8g0389741 42.353 85 49 0 28 112 368 452 3.92e-21 87.0
Msa0094320 MtrunA17_Chr5g0401301 47.674 86 44 1 28 112 329 414 8.16e-21 85.9
Msa0094320 MtrunA17_Chr2g0304061 48.276 87 43 2 27 113 157 241 9.08e-21 85.1
Msa0094320 MtrunA17_Chr8g0381081 38.384 99 58 1 18 113 44 142 9.89e-21 82.0
Msa0094320 MtrunA17_Chr3g0096451 44.186 86 47 1 27 112 111 195 1.49e-19 80.9
Msa0094320 MtrunA17_Chr5g0439251 46.835 79 42 0 28 106 382 460 1.55e-19 82.4
Msa0094320 MtrunA17_Chr7g0234821 41.176 85 50 0 28 112 374 458 1.84e-19 82.0
Msa0094320 MtrunA17_Chr7g0271411 41.860 86 49 1 28 113 160 244 3.99e-19 80.1
Msa0094320 MtrunA17_Chr1g0161011 42.353 85 49 0 28 112 361 445 4.98e-19 80.9
Msa0094320 MtrunA17_Chr8g0378371 35.714 112 68 2 1 112 6 113 1.37e-18 75.1
Msa0094320 MtrunA17_Chr8g0339191 37.647 85 53 0 28 112 374 458 3.91e-18 78.6
Msa0094320 MtrunA17_Chr5g0443331 41.860 86 49 1 27 112 107 191 6.71e-18 76.6
Msa0094320 MtrunA17_Chr3g0110371 42.353 85 49 0 28 112 361 445 6.90e-18 77.8
Msa0094320 MtrunA17_Chr6g0487261 39.080 87 52 1 27 112 368 454 7.39e-18 77.8
Msa0094320 MtrunA17_Chr5g0419121 34.906 106 68 1 9 114 3 107 7.82e-18 76.3
Msa0094320 MtrunA17_Chr5g0434591 41.176 85 50 0 28 112 360 444 8.34e-18 77.4
Msa0094320 MtrunA17_Chr8g0378381 34.259 108 67 2 5 112 12 115 8.85e-18 72.8
Msa0094320 MtrunA17_Chr1g0193131 45.977 87 45 2 22 108 414 498 1.10e-17 77.4
Msa0094320 MtrunA17_Chr4g0043151 41.463 82 47 1 28 109 21 101 2.78e-16 70.9
Msa0094320 MtrunA17_Chr4g0061401 37.895 95 57 2 18 112 14 106 3.65e-16 70.9
Msa0094320 MtrunA17_Chr8g0378401 33.333 111 70 2 5 115 12 118 4.57e-16 68.6
Msa0094320 MtrunA17_Chr4g0043511 35.227 88 56 1 27 114 419 505 1.09e-15 71.6
Msa0094320 MtrunA17_Chr8g0380231 48.611 72 36 1 35 106 24 94 1.13e-15 67.0
Msa0094320 MtrunA17_Chr4g0046311 39.241 79 48 0 28 106 373 451 2.15e-15 70.9
Msa0094320 MtrunA17_Chr4g0034901 37.209 86 54 0 27 112 374 459 2.38e-15 70.5
Msa0094320 MtrunA17_Chr1g0154991 31.959 97 62 2 16 112 11 103 3.25e-12 58.5
Msa0094320 MtrunA17_Chr3g0144531 34.118 85 55 1 28 112 371 454 9.48e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0094320 AT5G35740.1 63.107 103 37 1 15 116 17 119 1.22e-48 151
Msa0094320 AT2G04910.2 63.000 100 37 0 17 116 4 103 5.77e-46 144
Msa0094320 AT2G04910.1 62.222 90 34 0 17 106 4 93 1.76e-39 128
Msa0094320 AT3G13560.4 50.588 85 42 0 28 112 362 446 6.84e-26 100
Msa0094320 AT3G13560.1 50.588 85 42 0 28 112 362 446 6.84e-26 100
Msa0094320 AT3G13560.2 50.588 85 42 0 28 112 362 446 6.84e-26 100
Msa0094320 AT3G13560.5 50.588 85 42 0 28 112 362 446 6.84e-26 100
Msa0094320 AT3G13560.3 50.588 85 42 0 28 112 362 446 6.84e-26 100
Msa0094320 AT3G58100.1 41.071 112 63 1 4 112 14 125 8.69e-26 95.5
Msa0094320 AT3G58100.2 41.071 112 63 1 4 112 90 201 6.75e-25 95.1
Msa0094320 AT4G29360.2 43.137 102 58 0 15 116 388 489 6.36e-24 95.5
Msa0094320 AT4G29360.1 43.137 102 58 0 15 116 378 479 6.43e-24 95.5
Msa0094320 AT1G11820.2 45.631 103 49 1 17 112 363 465 7.67e-24 95.1
Msa0094320 AT1G29380.1 51.724 87 39 2 27 112 147 231 1.89e-23 92.4
Msa0094320 AT1G29380.3 51.136 88 40 2 27 113 147 232 1.93e-23 91.3
Msa0094320 AT2G05790.1 45.882 85 46 0 28 112 384 468 2.51e-23 93.6
Msa0094320 AT2G30933.1 43.925 107 55 2 10 112 60 165 3.68e-23 89.7
Msa0094320 AT4G05430.1 39.175 97 56 1 20 113 12 108 8.70e-23 86.7
Msa0094320 AT4G05430.3 39.175 97 56 1 20 113 37 133 1.46e-22 87.0
Msa0094320 AT1G79480.1 43.678 87 48 1 28 114 311 396 3.73e-22 89.7
Msa0094320 AT1G79480.2 43.678 87 48 1 28 114 310 395 4.06e-22 89.7
Msa0094320 AT5G67460.1 44.706 85 47 0 28 112 293 377 5.59e-22 89.0
Msa0094320 AT4G26830.2 42.353 85 49 0 28 112 371 455 8.80e-22 89.0
Msa0094320 AT4G26830.1 42.353 85 49 0 28 112 367 451 9.21e-22 89.0
Msa0094320 AT5G55180.1 40.625 96 57 0 17 112 361 456 1.73e-21 88.2
Msa0094320 AT5G56590.1 41.176 85 50 0 28 112 369 453 6.95e-21 86.7
Msa0094320 AT4G13600.1 44.706 85 46 1 28 112 61 144 8.69e-21 84.0
Msa0094320 AT1G29380.4 50.000 84 39 2 27 109 147 228 1.24e-20 83.2
Msa0094320 AT1G29380.2 50.000 84 39 2 27 109 147 228 1.24e-20 83.2
Msa0094320 AT2G30933.2 42.574 101 53 2 10 106 60 159 2.05e-20 81.3
Msa0094320 AT2G30933.3 42.574 101 53 2 10 106 60 159 2.05e-20 81.3
Msa0094320 AT5G63240.1 40.000 95 55 1 20 112 32 126 2.63e-20 80.1
Msa0094320 AT5G63250.1 41.379 87 49 1 28 112 42 128 6.61e-20 79.0
Msa0094320 AT5G67460.2 45.570 79 43 0 28 106 293 371 8.43e-20 83.2
Msa0094320 AT4G05430.2 40.000 90 51 1 20 106 37 126 8.80e-20 78.6
Msa0094320 AT1G11820.3 43.299 97 48 1 17 106 363 459 1.48e-19 82.8
Msa0094320 AT1G66855.1 34.821 112 71 1 1 112 1 110 1.53e-19 77.4
Msa0094320 AT1G18650.2 36.111 108 65 2 5 112 1 104 2.21e-19 77.8
Msa0094320 AT1G18650.1 36.111 108 65 2 5 112 1 104 3.63e-19 78.6
Msa0094320 AT5G55180.2 41.111 90 53 0 17 106 361 450 5.97e-19 81.3
Msa0094320 AT2G01630.1 42.353 85 49 0 28 112 360 444 7.31e-19 80.9
Msa0094320 AT1G66870.2 32.710 107 70 1 6 112 33 137 5.13e-18 74.3
Msa0094320 AT1G66870.1 31.250 112 75 1 1 112 1 110 5.74e-18 73.6
Msa0094320 AT1G66250.1 40.000 85 51 0 28 112 368 452 1.12e-17 77.8
Msa0094320 AT1G66852.1 33.333 105 67 2 8 112 8 109 1.41e-16 70.1
Msa0094320 AT4G16165.1 30.973 113 76 1 1 113 1 111 3.71e-16 68.9
Msa0094320 AT2G01630.3 41.772 79 46 0 28 106 360 438 5.14e-16 72.8
Msa0094320 AT3G07320.1 37.647 85 53 0 28 112 371 455 6.57e-16 72.4
Msa0094320 AT1G26450.1 37.234 94 55 2 6 99 1 90 8.86e-16 70.1
Msa0094320 AT5G63230.1 40.698 86 50 1 27 112 25 109 1.39e-15 67.4
Msa0094320 AT3G55430.1 32.632 95 60 2 18 112 359 449 2.31e-15 70.9
Msa0094320 AT3G23770.1 34.118 85 56 0 28 112 387 471 3.25e-15 70.5
Msa0094320 AT1G69295.1 37.500 104 61 3 6 109 1 100 5.03e-15 68.6
Msa0094320 AT5G08000.2 31.250 112 76 1 1 112 13 123 5.62e-15 68.2
Msa0094320 AT5G63225.1 34.821 112 69 2 1 112 1 108 6.15e-15 65.9
Msa0094320 AT1G69295.2 41.176 85 48 2 25 109 14 96 7.61e-15 67.8
Msa0094320 AT1G09460.1 42.391 92 48 3 22 113 133 219 7.99e-15 68.9
Msa0094320 AT4G09462.1 30.973 113 75 2 1 113 4 113 1.10e-14 65.1
Msa0094320 AT5G24318.1 34.884 86 55 1 28 113 372 456 1.12e-14 68.9
Msa0094320 AT4G09465.1 29.091 110 75 2 4 113 9 115 2.38e-14 64.3
Msa0094320 AT4G09464.1 29.091 110 75 2 4 113 9 115 2.95e-14 64.3
Msa0094320 AT4G09467.1 29.091 110 75 2 4 113 9 115 2.95e-14 64.3
Msa0094320 AT2G03505.3 39.241 79 47 1 28 106 21 98 5.24e-14 63.5
Msa0094320 AT5G24318.3 34.483 87 56 1 27 113 232 317 5.26e-14 66.6
Msa0094320 AT5G61130.1 33.721 86 56 1 27 112 20 104 7.16e-14 65.1
Msa0094320 AT2G03505.4 39.241 79 47 1 28 106 21 98 7.50e-14 64.3
Msa0094320 AT2G03505.1 39.241 79 47 1 28 106 21 98 7.50e-14 64.3
Msa0094320 AT2G03505.2 39.241 79 47 1 28 106 21 98 7.50e-14 64.3
Msa0094320 AT2G29735.1 33.929 112 65 5 6 113 11 117 2.24e-13 62.0
Msa0094320 AT4G09466.1 28.182 110 76 2 4 113 9 115 2.74e-13 61.6
Msa0094320 AT4G34480.1 32.941 85 56 1 28 112 364 447 3.10e-13 65.1
Msa0094320 AT4G34480.3 32.941 85 56 1 28 112 253 336 4.11e-13 64.7
Msa0094320 AT4G09090.1 28.440 109 75 2 4 112 9 114 4.69e-13 61.2
Msa0094320 AT5G20870.1 34.783 92 54 3 24 112 371 459 5.99e-13 64.3
Msa0094320 AT5G53610.1 32.110 109 69 2 4 112 7 110 6.08e-13 60.5
Msa0094320 AT5G53600.1 35.632 87 52 2 27 112 28 111 1.75e-12 59.3
Msa0094320 AT4G14080.1 29.412 85 60 0 28 112 389 473 2.58e-12 62.4
Msa0094320 AT2G16230.1 32.184 87 58 1 26 112 364 449 6.05e-12 61.2
Msa0094320 AT2G43670.1 31.818 88 58 1 27 114 34 119 1.38e-11 57.4
Msa0094320 AT5G24318.2 35.443 79 50 1 28 106 372 449 1.60e-11 60.1
Msa0094320 AT2G43660.2 32.632 95 63 1 20 114 28 121 5.36e-11 55.8
Msa0094320 AT2G43660.1 32.632 95 63 1 20 114 27 120 5.84e-11 55.8

Find 45 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGGTCCTTCATAGTATT+TGG 0.188691 1_2:+77530291 None:intergenic
ATAGAATGTAGTTGGCATTT+AGG 0.196616 1_2:+77529560 None:intergenic
CATAGTATTTGGAAGGTAAC+AGG 0.246733 1_2:+77530302 None:intergenic
TAGAATGTAGTTGGCATTTA+GGG 0.285616 1_2:+77529561 None:intergenic
GAAAATTTCAGGACAATTCA+TGG 0.287986 1_2:-77530433 Msa0094320:intron
TGAGGAACTCATGGTTCTTC+AGG 0.298592 1_2:+77530656 None:intergenic
ATGTATATTATGTCATTATA+AGG 0.303665 1_2:-77530710 Msa0094320:five_prime_UTR
TATATCCCTTGAATGAAGTT+TGG 0.309961 1_2:-77529758 Msa0094320:exon
TAAATGCCAACTACATTCTA+TGG 0.342090 1_2:-77529558 Msa0094320:three_prime_UTR
ATATCCCTTGAATGAAGTTT+GGG 0.347148 1_2:-77529757 Msa0094320:exon
GCCCTCTGCAGATCATCATC+TGG 0.401963 1_2:+77530384 None:intergenic
CAAGTGACATAAGGCATTGC+TGG 0.424094 1_2:+77529529 None:intergenic
ATGGATTGGGCTTGTCATGT+TGG 0.425033 1_2:-77530360 Msa0094320:CDS
ATCTGCAGAGGGCTATGGAT+TGG 0.450182 1_2:-77530374 Msa0094320:CDS
ATTACCCCAAACTTCATTCA+AGG 0.455553 1_2:+77529752 None:intergenic
TCTGCAGAGGGCTATGGATT+GGG 0.456199 1_2:-77530373 Msa0094320:CDS
CCCTCTGCAGATCATCATCT+GGG 0.469613 1_2:+77530385 None:intergenic
ACCTGATGCTTGAAAAGGTA+GGG 0.472261 1_2:+77530599 None:intergenic
TGATGATCTGCAGAGGGCTA+TGG 0.487175 1_2:-77530379 Msa0094320:CDS
TTTGATCATTGAGGAACTCA+TGG 0.502590 1_2:+77530647 None:intergenic
TTTCAGGACAATTCATGGAA+TGG 0.512225 1_2:-77530428 Msa0094320:intron
GATTGGGCTTGTCATGTTGG+TGG 0.526500 1_2:-77530357 Msa0094320:CDS
TTACCTTCCAAATACTATGA+AGG 0.531309 1_2:-77530298 Msa0094320:CDS
TTTACTTGGATCAAGATCAC+TGG 0.543257 1_2:+77530191 None:intergenic
TATTTGGAAGGTAACAGGGT+TGG 0.547742 1_2:+77530307 None:intergenic
ATAGTAATTGTTGAAGACAT+AGG 0.556410 1_2:+77530266 None:intergenic
TTCACACCATAGAATGTAGT+TGG 0.556550 1_2:+77529552 None:intergenic
CTTGAAAAGGTAGGGTCACA+AGG 0.557030 1_2:+77530607 None:intergenic
GTTGGCATTTAGGGTGGGCA+AGG 0.558771 1_2:+77529570 None:intergenic
TTACCCCAAACTTCATTCAA+GGG 0.566615 1_2:+77529753 None:intergenic
AATGTAGTTGGCATTTAGGG+TGG 0.571985 1_2:+77529564 None:intergenic
GGTCCTTCATAGTATTTGGA+AGG 0.583934 1_2:+77530295 None:intergenic
TGTTGAAGACATAGGAGGCA+TGG 0.588471 1_2:+77530274 None:intergenic
ATGTAGTTGGCATTTAGGGT+GGG 0.589738 1_2:+77529565 None:intergenic
ATAGTATTTGGAAGGTAACA+GGG 0.607638 1_2:+77530303 None:intergenic
TACCTGATGCTTGAAAAGGT+AGG 0.609047 1_2:+77530598 None:intergenic
CAGAAGTTCAAGCACAAAGG+AGG 0.618787 1_2:-77530243 Msa0094320:CDS
GATATGAAGTAGTATTAGCA+TGG 0.620936 1_2:-77529647 Msa0094320:three_prime_UTR
TTATAAGGAATATAGTTGCA+AGG 0.622058 1_2:-77530695 Msa0094320:five_prime_UTR
CCCAGATGATGATCTGCAGA+GGG 0.624946 1_2:-77530385 Msa0094320:CDS
GTAATTGTTGAAGACATAGG+AGG 0.633395 1_2:+77530269 None:intergenic
CCCCAGATGATGATCTGCAG+AGG 0.639650 1_2:-77530386 Msa0094320:CDS
TATCAGAAGTTCAAGCACAA+AGG 0.651785 1_2:-77530246 Msa0094320:CDS
CCTCTGCAGATCATCATCTG+GGG 0.680867 1_2:+77530386 None:intergenic
TATCCCTTGAATGAAGTTTG+GGG 0.706452 1_2:-77529756 Msa0094320:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTCTCAAATTTTTATT+TGG - chr1_2:77530287-77530306 Msa0094320:CDS 10.0%
!!! AATTTTCTCAAATTTTTATT+TGG - chr1_2:77530287-77530306 Msa0094320:CDS 10.0%
!! ATGTATATTATGTCATTATA+AGG - chr1_2:77529560-77529579 Msa0094320:three_prime_UTR 15.0%
!!! TTATTTTTCTGCAATTTAAA+TGG - chr1_2:77530666-77530685 Msa0094320:exon 15.0%
!! ATGTATATTATGTCATTATA+AGG - chr1_2:77529560-77529579 Msa0094320:three_prime_UTR 15.0%
!!! TTATTTTTCTGCAATTTAAA+TGG - chr1_2:77530666-77530685 Msa0094320:exon 15.0%
!!! TATCAAAATTTTGATCATTG+AGG + chr1_2:77529635-77529654 None:intergenic 20.0%
!! GTCATTTAAGTAAAATCAAA+AGG + chr1_2:77530391-77530410 None:intergenic 20.0%
!!! GTTTTTCTTTGTTTAATTAC+AGG - chr1_2:77530467-77530486 Msa0094320:intron 20.0%
!!! TATCAAAATTTTGATCATTG+AGG + chr1_2:77529635-77529654 None:intergenic 20.0%
!! GTCATTTAAGTAAAATCAAA+AGG + chr1_2:77530391-77530410 None:intergenic 20.0%
!!! GTTTTTCTTTGTTTAATTAC+AGG - chr1_2:77530467-77530486 Msa0094320:intron 20.0%
! TTATAAGGAATATAGTTGCA+AGG - chr1_2:77529575-77529594 Msa0094320:three_prime_UTR 25.0%
! ACGTATTATTGGAAAATTTC+AGG - chr1_2:77529826-77529845 Msa0094320:intron 25.0%
! ATAGTAATTGTTGAAGACAT+AGG + chr1_2:77530007-77530026 None:intergenic 25.0%
!!! ATTATGAGAGAGTATTTACT+TGG + chr1_2:77530096-77530115 None:intergenic 25.0%
! TTGACTGTTACTATATGTAT+TGG + chr1_2:77530261-77530280 None:intergenic 25.0%
! TTCATAGATGAATACCATAT+GGG - chr1_2:77530356-77530375 Msa0094320:CDS 25.0%
! CAAAGAAAAACCATATCATT+TGG + chr1_2:77530458-77530477 None:intergenic 25.0%
! TTATAAGGAATATAGTTGCA+AGG - chr1_2:77529575-77529594 Msa0094320:three_prime_UTR 25.0%
! ACGTATTATTGGAAAATTTC+AGG - chr1_2:77529826-77529845 Msa0094320:intron 25.0%
! ATAGTAATTGTTGAAGACAT+AGG + chr1_2:77530007-77530026 None:intergenic 25.0%
!!! ATTATGAGAGAGTATTTACT+TGG + chr1_2:77530096-77530115 None:intergenic 25.0%
! TTGACTGTTACTATATGTAT+TGG + chr1_2:77530261-77530280 None:intergenic 25.0%
! TTCATAGATGAATACCATAT+GGG - chr1_2:77530356-77530375 Msa0094320:CDS 25.0%
! CAAAGAAAAACCATATCATT+TGG + chr1_2:77530458-77530477 None:intergenic 25.0%
! ATTTTACCTGATGCTTGAAA+AGG + chr1_2:77529679-77529698 None:intergenic 30.0%
ACTTGGAAGAACATAAATGA+GGG + chr1_2:77529710-77529729 None:intergenic 30.0%
AACTTGGAAGAACATAAATG+AGG + chr1_2:77529711-77529730 None:intergenic 30.0%
! TGATGATGATGACGTATTAT+TGG - chr1_2:77529815-77529834 Msa0094320:intron 30.0%
GAAAATTTCAGGACAATTCA+TGG - chr1_2:77529837-77529856 Msa0094320:intron 30.0%
ATAGTATTTGGAAGGTAACA+GGG + chr1_2:77529970-77529989 None:intergenic 30.0%
TTACCTTCCAAATACTATGA+AGG - chr1_2:77529972-77529991 Msa0094320:intron 30.0%
GTTCATAGATGAATACCATA+TGG - chr1_2:77530355-77530374 Msa0094320:CDS 30.0%
CATTATCAGTCCAAATGATA+TGG - chr1_2:77530445-77530464 Msa0094320:intron 30.0%
! CTTTGTTTAATTACAGGTCA+TGG - chr1_2:77530473-77530492 Msa0094320:intron 30.0%
TATATCCCTTGAATGAAGTT+TGG - chr1_2:77530512-77530531 Msa0094320:intron 30.0%
ATATCCCTTGAATGAAGTTT+GGG - chr1_2:77530513-77530532 Msa0094320:intron 30.0%
GATATGAAGTAGTATTAGCA+TGG - chr1_2:77530623-77530642 Msa0094320:CDS 30.0%
TAGAATGTAGTTGGCATTTA+GGG + chr1_2:77530712-77530731 None:intergenic 30.0%
ATAGAATGTAGTTGGCATTT+AGG + chr1_2:77530713-77530732 None:intergenic 30.0%
TAAATGCCAACTACATTCTA+TGG - chr1_2:77530712-77530731 Msa0094320:five_prime_UTR 30.0%
! ATTTTACCTGATGCTTGAAA+AGG + chr1_2:77529679-77529698 None:intergenic 30.0%
ACTTGGAAGAACATAAATGA+GGG + chr1_2:77529710-77529729 None:intergenic 30.0%
AACTTGGAAGAACATAAATG+AGG + chr1_2:77529711-77529730 None:intergenic 30.0%
! TGATGATGATGACGTATTAT+TGG - chr1_2:77529815-77529834 Msa0094320:intron 30.0%
GAAAATTTCAGGACAATTCA+TGG - chr1_2:77529837-77529856 Msa0094320:intron 30.0%
ATAGTATTTGGAAGGTAACA+GGG + chr1_2:77529970-77529989 None:intergenic 30.0%
TTACCTTCCAAATACTATGA+AGG - chr1_2:77529972-77529991 Msa0094320:intron 30.0%
GTTCATAGATGAATACCATA+TGG - chr1_2:77530355-77530374 Msa0094320:CDS 30.0%
CATTATCAGTCCAAATGATA+TGG - chr1_2:77530445-77530464 Msa0094320:intron 30.0%
! CTTTGTTTAATTACAGGTCA+TGG - chr1_2:77530473-77530492 Msa0094320:intron 30.0%
TATATCCCTTGAATGAAGTT+TGG - chr1_2:77530512-77530531 Msa0094320:intron 30.0%
ATATCCCTTGAATGAAGTTT+GGG - chr1_2:77530513-77530532 Msa0094320:intron 30.0%
GATATGAAGTAGTATTAGCA+TGG - chr1_2:77530623-77530642 Msa0094320:CDS 30.0%
TAGAATGTAGTTGGCATTTA+GGG + chr1_2:77530712-77530731 None:intergenic 30.0%
ATAGAATGTAGTTGGCATTT+AGG + chr1_2:77530713-77530732 None:intergenic 30.0%
TAAATGCCAACTACATTCTA+TGG - chr1_2:77530712-77530731 Msa0094320:five_prime_UTR 30.0%
TTTGATCATTGAGGAACTCA+TGG + chr1_2:77529626-77529645 None:intergenic 35.0%
CTAGCTGAATGTGATAAACT+TGG + chr1_2:77529727-77529746 None:intergenic 35.0%
ATTCAGCTAGAGAGTTTACT+TGG - chr1_2:77529737-77529756 Msa0094320:three_prime_UTR 35.0%
TTTCAGGACAATTCATGGAA+TGG - chr1_2:77529842-77529861 Msa0094320:intron 35.0%
CATAGTATTTGGAAGGTAAC+AGG + chr1_2:77529971-77529990 None:intergenic 35.0%
GTAATTGTTGAAGACATAGG+AGG + chr1_2:77530004-77530023 None:intergenic 35.0%
TATCAGAAGTTCAAGCACAA+AGG - chr1_2:77530024-77530043 Msa0094320:intron 35.0%
TTTACTTGGATCAAGATCAC+TGG + chr1_2:77530082-77530101 None:intergenic 35.0%
ACTTACACATACACCAACAA+AGG + chr1_2:77530335-77530354 None:intergenic 35.0%
TATCCCTTGAATGAAGTTTG+GGG - chr1_2:77530514-77530533 Msa0094320:intron 35.0%
TTACCCCAAACTTCATTCAA+GGG + chr1_2:77530520-77530539 None:intergenic 35.0%
ATTACCCCAAACTTCATTCA+AGG + chr1_2:77530521-77530540 None:intergenic 35.0%
TTCACACCATAGAATGTAGT+TGG + chr1_2:77530721-77530740 None:intergenic 35.0%
TTTGATCATTGAGGAACTCA+TGG + chr1_2:77529626-77529645 None:intergenic 35.0%
CTAGCTGAATGTGATAAACT+TGG + chr1_2:77529727-77529746 None:intergenic 35.0%
ATTCAGCTAGAGAGTTTACT+TGG - chr1_2:77529737-77529756 Msa0094320:three_prime_UTR 35.0%
TTTCAGGACAATTCATGGAA+TGG - chr1_2:77529842-77529861 Msa0094320:intron 35.0%
CATAGTATTTGGAAGGTAAC+AGG + chr1_2:77529971-77529990 None:intergenic 35.0%
GTAATTGTTGAAGACATAGG+AGG + chr1_2:77530004-77530023 None:intergenic 35.0%
TATCAGAAGTTCAAGCACAA+AGG - chr1_2:77530024-77530043 Msa0094320:intron 35.0%
TTTACTTGGATCAAGATCAC+TGG + chr1_2:77530082-77530101 None:intergenic 35.0%
ACTTACACATACACCAACAA+AGG + chr1_2:77530335-77530354 None:intergenic 35.0%
TATCCCTTGAATGAAGTTTG+GGG - chr1_2:77530514-77530533 Msa0094320:intron 35.0%
TTACCCCAAACTTCATTCAA+GGG + chr1_2:77530520-77530539 None:intergenic 35.0%
ATTACCCCAAACTTCATTCA+AGG + chr1_2:77530521-77530540 None:intergenic 35.0%
TTCACACCATAGAATGTAGT+TGG + chr1_2:77530721-77530740 None:intergenic 35.0%
ACCTGATGCTTGAAAAGGTA+GGG + chr1_2:77529674-77529693 None:intergenic 40.0%
TACCTGATGCTTGAAAAGGT+AGG + chr1_2:77529675-77529694 None:intergenic 40.0%
TATTTGGAAGGTAACAGGGT+TGG + chr1_2:77529966-77529985 None:intergenic 40.0%
GGTCCTTCATAGTATTTGGA+AGG + chr1_2:77529978-77529997 None:intergenic 40.0%
GCATGGTCCTTCATAGTATT+TGG + chr1_2:77529982-77530001 None:intergenic 40.0%
!! GAGAAGTGTTGGTTCTATAG+AGG + chr1_2:77530186-77530205 None:intergenic 40.0%
AGTGTGACACTAACACGTAT+TGG + chr1_2:77530229-77530248 None:intergenic 40.0%
!! AAAGGCTTTTGAGTCCCATA+TGG + chr1_2:77530373-77530392 None:intergenic 40.0%
! ATGTAGTTGGCATTTAGGGT+GGG + chr1_2:77530708-77530727 None:intergenic 40.0%
AATGTAGTTGGCATTTAGGG+TGG + chr1_2:77530709-77530728 None:intergenic 40.0%
ACCTGATGCTTGAAAAGGTA+GGG + chr1_2:77529674-77529693 None:intergenic 40.0%
TACCTGATGCTTGAAAAGGT+AGG + chr1_2:77529675-77529694 None:intergenic 40.0%
TATTTGGAAGGTAACAGGGT+TGG + chr1_2:77529966-77529985 None:intergenic 40.0%
GGTCCTTCATAGTATTTGGA+AGG + chr1_2:77529978-77529997 None:intergenic 40.0%
GCATGGTCCTTCATAGTATT+TGG + chr1_2:77529982-77530001 None:intergenic 40.0%
!! GAGAAGTGTTGGTTCTATAG+AGG + chr1_2:77530186-77530205 None:intergenic 40.0%
AGTGTGACACTAACACGTAT+TGG + chr1_2:77530229-77530248 None:intergenic 40.0%
!! AAAGGCTTTTGAGTCCCATA+TGG + chr1_2:77530373-77530392 None:intergenic 40.0%
! ATGTAGTTGGCATTTAGGGT+GGG + chr1_2:77530708-77530727 None:intergenic 40.0%
AATGTAGTTGGCATTTAGGG+TGG + chr1_2:77530709-77530728 None:intergenic 40.0%
TGAGGAACTCATGGTTCTTC+AGG + chr1_2:77529617-77529636 None:intergenic 45.0%
CTTGAAAAGGTAGGGTCACA+AGG + chr1_2:77529666-77529685 None:intergenic 45.0%
!! ACCCTACCTTTTCAAGCATC+AGG - chr1_2:77529670-77529689 Msa0094320:three_prime_UTR 45.0%
!! ATGGATTGGGCTTGTCATGT+TGG - chr1_2:77529910-77529929 Msa0094320:intron 45.0%
TGTTGAAGACATAGGAGGCA+TGG + chr1_2:77529999-77530018 None:intergenic 45.0%
CAGAAGTTCAAGCACAAAGG+AGG - chr1_2:77530027-77530046 Msa0094320:intron 45.0%
! ACATCTTCGAGGAGAAGTGT+TGG + chr1_2:77530197-77530216 None:intergenic 45.0%
!! GAGTCGACTTGTCAGTATCA+CGG - chr1_2:77530309-77530328 Msa0094320:CDS 45.0%
TGAGGAACTCATGGTTCTTC+AGG + chr1_2:77529617-77529636 None:intergenic 45.0%
CTTGAAAAGGTAGGGTCACA+AGG + chr1_2:77529666-77529685 None:intergenic 45.0%
!! ACCCTACCTTTTCAAGCATC+AGG - chr1_2:77529670-77529689 Msa0094320:three_prime_UTR 45.0%
!! ATGGATTGGGCTTGTCATGT+TGG - chr1_2:77529910-77529929 Msa0094320:intron 45.0%
TGTTGAAGACATAGGAGGCA+TGG + chr1_2:77529999-77530018 None:intergenic 45.0%
CAGAAGTTCAAGCACAAAGG+AGG - chr1_2:77530027-77530046 Msa0094320:intron 45.0%
! ACATCTTCGAGGAGAAGTGT+TGG + chr1_2:77530197-77530216 None:intergenic 45.0%
!! GAGTCGACTTGTCAGTATCA+CGG - chr1_2:77530309-77530328 Msa0094320:CDS 45.0%
CCTCTGCAGATCATCATCTG+GGG + chr1_2:77529887-77529906 None:intergenic 50.0%
CCCTCTGCAGATCATCATCT+GGG + chr1_2:77529888-77529907 None:intergenic 50.0%
CCCAGATGATGATCTGCAGA+GGG - chr1_2:77529885-77529904 Msa0094320:intron 50.0%
TGATGATCTGCAGAGGGCTA+TGG - chr1_2:77529891-77529910 Msa0094320:intron 50.0%
ATCTGCAGAGGGCTATGGAT+TGG - chr1_2:77529896-77529915 Msa0094320:intron 50.0%
TCTGCAGAGGGCTATGGATT+GGG - chr1_2:77529897-77529916 Msa0094320:intron 50.0%
GATTGGGCTTGTCATGTTGG+TGG - chr1_2:77529913-77529932 Msa0094320:intron 50.0%
GGACACTGAACACATCTTCG+AGG + chr1_2:77530208-77530227 None:intergenic 50.0%
GTCAGTATCACGGCCTTTGT+TGG - chr1_2:77530319-77530338 Msa0094320:CDS 50.0%
CCTCTGCAGATCATCATCTG+GGG + chr1_2:77529887-77529906 None:intergenic 50.0%
CCCTCTGCAGATCATCATCT+GGG + chr1_2:77529888-77529907 None:intergenic 50.0%
CCCAGATGATGATCTGCAGA+GGG - chr1_2:77529885-77529904 Msa0094320:intron 50.0%
TGATGATCTGCAGAGGGCTA+TGG - chr1_2:77529891-77529910 Msa0094320:intron 50.0%
ATCTGCAGAGGGCTATGGAT+TGG - chr1_2:77529896-77529915 Msa0094320:intron 50.0%
TCTGCAGAGGGCTATGGATT+GGG - chr1_2:77529897-77529916 Msa0094320:intron 50.0%
GATTGGGCTTGTCATGTTGG+TGG - chr1_2:77529913-77529932 Msa0094320:intron 50.0%
GGACACTGAACACATCTTCG+AGG + chr1_2:77530208-77530227 None:intergenic 50.0%
GTCAGTATCACGGCCTTTGT+TGG - chr1_2:77530319-77530338 Msa0094320:CDS 50.0%
CCCCAGATGATGATCTGCAG+AGG - chr1_2:77529884-77529903 Msa0094320:intron 55.0%
GCCCTCTGCAGATCATCATC+TGG + chr1_2:77529889-77529908 None:intergenic 55.0%
! GTTGGCATTTAGGGTGGGCA+AGG + chr1_2:77530703-77530722 None:intergenic 55.0%
CCCCAGATGATGATCTGCAG+AGG - chr1_2:77529884-77529903 Msa0094320:intron 55.0%
GCCCTCTGCAGATCATCATC+TGG + chr1_2:77529889-77529908 None:intergenic 55.0%
! GTTGGCATTTAGGGTGGGCA+AGG + chr1_2:77530703-77530722 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 77529536 77530756 77529536 ID=Msa0094320;Name=Msa0094320
chr1_2 mRNA 77529536 77530756 77529536 ID=Msa0094320-mRNA-1;Parent=Msa0094320;Name=Msa0094320-mRNA-1;_AED=0.25;_eAED=0.25;_QI=89|1|1|1|1|1|3|233|116
chr1_2 exon 77529536 77529803 77529536 ID=Msa0094320-mRNA-1:exon:27523;Parent=Msa0094320-mRNA-1
chr1_2 exon 77530196 77530444 77530196 ID=Msa0094320-mRNA-1:exon:27522;Parent=Msa0094320-mRNA-1
chr1_2 exon 77530601 77530756 77530601 ID=Msa0094320-mRNA-1:exon:27521;Parent=Msa0094320-mRNA-1
chr1_2 five_prime_UTR 77530668 77530756 77530668 ID=Msa0094320-mRNA-1:five_prime_utr;Parent=Msa0094320-mRNA-1
chr1_2 CDS 77530601 77530667 77530601 ID=Msa0094320-mRNA-1:cds;Parent=Msa0094320-mRNA-1
chr1_2 CDS 77530196 77530444 77530196 ID=Msa0094320-mRNA-1:cds;Parent=Msa0094320-mRNA-1
chr1_2 CDS 77529769 77529803 77529769 ID=Msa0094320-mRNA-1:cds;Parent=Msa0094320-mRNA-1
chr1_2 three_prime_UTR 77529536 77529768 77529536 ID=Msa0094320-mRNA-1:three_prime_utr;Parent=Msa0094320-mRNA-1
Gene Sequence

>Msa0094320

ATGAGTTCCTCAATGATCAAAATTTTGATAGCTATGCTCCTTGTGACCCTACCTTTTCAAGCATCAGGACAATTCATGGAATGGTGCATAGCTGATGAGCAGACCCCAGATGATGATCTGCAGAGGGCTATGGATTGGGCTTGTCATGTTGGTGGAGCAAATTGTAGCAACATACAAGTGAACCAACCCTGTTACCTTCCAAATACTATGAAGGACCATGCCTCCTATGTCTTCAACAATTACTATCAGAAGTTCAAGCACAAAGGAGGATCTTGCTATTTTAACTCTGCTGCAATAACCAGTGATCTTGATCCAAGTCATGGCTCTTGCAAGTTTGAGTATATCCCTTGA
Protein sequence

>Msa0094320

MSSSMIKILIAMLLVTLPFQASGQFMEWCIADEQTPDDDLQRAMDWACHVGGANCSNIQVNQPCYLPNTMKDHASYVFNNYYQKFKHKGGSCYFNSAAITSDLDPSHGSCKFEYIP*