AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1224230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1224230 MtrunA17_Chr4g0076131 95.690 116 5 0 1 116 1 116 1.07e-83 239
Msa1224230 MtrunA17_Chr4g0076141 72.165 97 27 0 19 115 4 100 7.81e-49 150
Msa1224230 MtrunA17_Chr5g0447651 55.932 118 50 1 1 116 1 118 1.00e-48 151
Msa1224230 MtrunA17_Chr8g0378621 50.588 85 42 0 28 112 364 448 1.06e-25 99.8
Msa1224230 MtrunA17_Chr3g0121271 45.349 86 47 0 27 112 361 446 1.38e-24 96.7
Msa1224230 MtrunA17_Chr4g0075031 51.765 85 41 0 28 112 382 466 2.47e-24 96.3
Msa1224230 MtrunA17_Chr4g0040911 47.059 85 45 0 28 112 37 121 4.04e-23 88.2
Msa1224230 MtrunA17_Chr1g0213651 44.828 87 47 1 28 114 276 361 2.03e-22 89.7
Msa1224230 MtrunA17_Chr8g0378361 38.095 105 64 1 8 112 10 113 3.20e-22 86.3
Msa1224230 MtrunA17_Chr3g0120401 44.444 90 49 1 24 113 153 241 3.77e-22 87.4
Msa1224230 MtrunA17_Chr1g0177171 44.706 85 47 0 28 112 406 490 1.69e-21 88.2
Msa1224230 MtrunA17_Chr8g0389741 42.353 85 49 0 28 112 368 452 3.92e-21 87.0
Msa1224230 MtrunA17_Chr5g0401301 47.674 86 44 1 28 112 329 414 7.24e-21 85.9
Msa1224230 MtrunA17_Chr2g0304061 48.276 87 43 2 27 113 157 241 9.08e-21 85.1
Msa1224230 MtrunA17_Chr8g0381081 38.384 99 58 1 18 113 44 142 1.15e-20 82.0
Msa1224230 MtrunA17_Chr3g0096451 44.186 86 47 1 27 112 111 195 1.54e-19 80.5
Msa1224230 MtrunA17_Chr5g0439251 46.835 79 42 0 28 106 382 460 1.57e-19 82.4
Msa1224230 MtrunA17_Chr7g0234821 41.176 85 50 0 28 112 374 458 1.80e-19 82.4
Msa1224230 MtrunA17_Chr7g0271411 41.860 86 49 1 28 113 160 244 4.07e-19 80.1
Msa1224230 MtrunA17_Chr1g0161011 42.353 85 49 0 28 112 361 445 4.30e-19 81.3
Msa1224230 MtrunA17_Chr8g0378371 36.364 110 66 2 3 112 8 113 1.55e-18 74.7
Msa1224230 MtrunA17_Chr8g0339191 37.647 85 53 0 28 112 374 458 3.31e-18 78.6
Msa1224230 MtrunA17_Chr3g0110371 42.353 85 49 0 28 112 361 445 6.63e-18 77.8
Msa1224230 MtrunA17_Chr5g0443331 41.860 86 49 1 27 112 107 191 7.14e-18 76.3
Msa1224230 MtrunA17_Chr6g0487261 39.080 87 52 1 27 112 368 454 7.76e-18 77.8
Msa1224230 MtrunA17_Chr1g0193131 45.977 87 45 2 22 108 414 498 7.91e-18 77.8
Msa1224230 MtrunA17_Chr5g0434591 41.176 85 50 0 28 112 360 444 8.10e-18 77.8
Msa1224230 MtrunA17_Chr5g0419121 36.893 103 62 2 12 114 8 107 8.41e-18 75.9
Msa1224230 MtrunA17_Chr8g0378381 34.259 108 67 2 5 112 12 115 9.96e-18 72.8
Msa1224230 MtrunA17_Chr4g0043151 41.463 82 47 1 28 109 21 101 2.78e-16 70.9
Msa1224230 MtrunA17_Chr4g0061401 37.895 95 57 2 18 112 14 106 3.85e-16 70.9
Msa1224230 MtrunA17_Chr8g0378401 33.333 111 70 2 5 115 12 118 4.33e-16 68.6
Msa1224230 MtrunA17_Chr4g0043511 35.227 88 56 1 27 114 419 505 1.08e-15 71.6
Msa1224230 MtrunA17_Chr8g0380231 48.611 72 36 1 35 106 24 94 1.15e-15 67.0
Msa1224230 MtrunA17_Chr4g0046311 39.241 79 48 0 28 106 373 451 1.97e-15 70.9
Msa1224230 MtrunA17_Chr4g0034901 37.209 86 54 0 27 112 374 459 2.53e-15 70.5
Msa1224230 MtrunA17_Chr1g0154991 31.959 97 62 2 16 112 11 103 3.47e-12 58.2
Msa1224230 MtrunA17_Chr3g0144531 34.118 85 55 1 28 112 371 454 1.01e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1224230 AT5G35740.1 63.107 103 37 1 15 116 17 119 1.24e-48 151
Msa1224230 AT2G04910.2 63.000 100 37 0 17 116 4 103 7.67e-46 144
Msa1224230 AT2G04910.1 62.222 90 34 0 17 106 4 93 2.26e-39 128
Msa1224230 AT3G13560.4 50.588 85 42 0 28 112 362 446 6.57e-26 100
Msa1224230 AT3G13560.1 50.588 85 42 0 28 112 362 446 6.57e-26 100
Msa1224230 AT3G13560.2 50.588 85 42 0 28 112 362 446 6.57e-26 100
Msa1224230 AT3G13560.5 50.588 85 42 0 28 112 362 446 6.57e-26 100
Msa1224230 AT3G13560.3 50.588 85 42 0 28 112 362 446 6.57e-26 100
Msa1224230 AT3G58100.1 41.071 112 63 1 4 112 14 125 1.06e-25 95.1
Msa1224230 AT3G58100.2 41.071 112 63 1 4 112 90 201 8.18e-25 94.7
Msa1224230 AT4G29360.1 43.137 102 58 0 15 116 378 479 5.29e-24 95.5
Msa1224230 AT4G29360.2 43.137 102 58 0 15 116 388 489 5.43e-24 95.5
Msa1224230 AT1G11820.2 45.631 103 49 1 17 112 363 465 6.68e-24 95.1
Msa1224230 AT1G29380.1 51.724 87 39 2 27 112 147 231 1.72e-23 92.4
Msa1224230 AT1G29380.3 51.136 88 40 2 27 113 147 232 1.76e-23 91.3
Msa1224230 AT2G05790.1 45.882 85 46 0 28 112 384 468 2.16e-23 93.6
Msa1224230 AT2G30933.1 43.925 107 55 2 10 112 60 165 4.05e-23 89.7
Msa1224230 AT4G05430.1 39.175 97 56 1 20 113 12 108 9.09e-23 86.7
Msa1224230 AT4G05430.3 39.175 97 56 1 20 113 37 133 1.76e-22 86.7
Msa1224230 AT1G79480.1 43.678 87 48 1 28 114 311 396 3.81e-22 89.7
Msa1224230 AT1G79480.2 43.678 87 48 1 28 114 310 395 4.02e-22 89.7
Msa1224230 AT5G67460.1 44.706 85 47 0 28 112 293 377 5.48e-22 89.4
Msa1224230 AT4G26830.2 42.353 85 49 0 28 112 371 455 9.33e-22 89.0
Msa1224230 AT4G26830.1 42.353 85 49 0 28 112 367 451 9.39e-22 89.0
Msa1224230 AT5G55180.1 40.625 96 57 0 17 112 361 456 2.01e-21 88.2
Msa1224230 AT5G56590.1 41.176 85 50 0 28 112 369 453 6.88e-21 86.7
Msa1224230 AT4G13600.1 44.706 85 46 1 28 112 61 144 7.98e-21 84.0
Msa1224230 AT1G29380.4 50.000 84 39 2 27 109 147 228 1.38e-20 83.2
Msa1224230 AT1G29380.2 50.000 84 39 2 27 109 147 228 1.38e-20 83.2
Msa1224230 AT2G30933.2 42.574 101 53 2 10 106 60 159 2.19e-20 81.3
Msa1224230 AT2G30933.3 42.574 101 53 2 10 106 60 159 2.19e-20 81.3
Msa1224230 AT5G63240.1 40.000 95 55 1 20 112 32 126 2.81e-20 80.1
Msa1224230 AT5G63250.1 41.379 87 49 1 28 112 42 128 7.78e-20 79.0
Msa1224230 AT5G67460.2 45.570 79 43 0 28 106 293 371 8.69e-20 83.2
Msa1224230 AT4G05430.2 40.000 90 51 1 20 106 37 126 9.29e-20 78.6
Msa1224230 AT1G11820.3 43.299 97 48 1 17 106 363 459 1.29e-19 83.2
Msa1224230 AT1G18650.2 36.111 108 65 2 5 112 1 104 2.05e-19 77.8
Msa1224230 AT1G66855.1 34.821 112 71 1 1 112 1 110 2.17e-19 77.0
Msa1224230 AT1G18650.1 36.111 108 65 2 5 112 1 104 3.75e-19 78.6
Msa1224230 AT2G01630.1 42.353 85 49 0 28 112 360 444 5.96e-19 81.3
Msa1224230 AT5G55180.2 41.111 90 53 0 17 106 361 450 7.12e-19 80.9
Msa1224230 AT1G66870.2 32.710 107 70 1 6 112 33 137 5.24e-18 74.3
Msa1224230 AT1G66870.1 31.250 112 75 1 1 112 1 110 5.80e-18 73.6
Msa1224230 AT1G66250.1 40.000 85 51 0 28 112 368 452 1.42e-17 77.4
Msa1224230 AT1G66852.1 33.333 105 67 2 8 112 8 109 1.51e-16 70.1
Msa1224230 AT2G01630.3 41.772 79 46 0 28 106 360 438 4.48e-16 73.2
Msa1224230 AT4G16165.1 30.973 113 76 1 1 113 1 111 4.66e-16 68.6
Msa1224230 AT3G07320.1 37.647 85 53 0 28 112 371 455 6.83e-16 72.4
Msa1224230 AT1G26450.1 37.234 94 55 2 6 99 1 90 9.14e-16 70.1
Msa1224230 AT5G63230.1 40.698 86 50 1 27 112 25 109 1.45e-15 67.4
Msa1224230 AT3G55430.1 32.632 95 60 2 18 112 359 449 2.68e-15 70.9
Msa1224230 AT1G69295.1 37.500 104 61 3 6 109 1 100 5.47e-15 68.6
Msa1224230 AT5G08000.2 31.776 107 72 1 6 112 18 123 6.78e-15 67.8
Msa1224230 AT1G69295.2 41.176 85 48 2 25 109 14 96 7.77e-15 67.8
Msa1224230 AT1G09460.1 42.391 92 48 3 22 113 133 219 7.99e-15 68.9
Msa1224230 AT3G23770.1 32.955 88 59 0 28 115 387 474 1.00e-14 69.3
Msa1224230 AT5G24318.1 34.884 86 55 1 28 113 372 456 1.14e-14 68.9
Msa1224230 AT4G09462.1 30.476 105 72 1 9 113 10 113 1.15e-14 65.1
Msa1224230 AT5G63225.1 36.735 98 58 2 15 112 15 108 1.59e-14 64.7
Msa1224230 AT4G09465.1 29.091 110 75 2 4 113 9 115 2.38e-14 64.3
Msa1224230 AT4G09464.1 29.091 110 75 2 4 113 9 115 3.18e-14 63.9
Msa1224230 AT4G09467.1 29.091 110 75 2 4 113 9 115 3.18e-14 63.9
Msa1224230 AT2G03505.3 39.241 79 47 1 28 106 21 98 5.53e-14 63.5
Msa1224230 AT5G24318.3 34.483 87 56 1 27 113 232 317 5.99e-14 66.6
Msa1224230 AT2G03505.4 39.241 79 47 1 28 106 21 98 7.66e-14 64.3
Msa1224230 AT2G03505.1 39.241 79 47 1 28 106 21 98 7.66e-14 64.3
Msa1224230 AT2G03505.2 39.241 79 47 1 28 106 21 98 7.66e-14 64.3
Msa1224230 AT5G61130.1 33.721 86 56 1 27 112 20 104 7.70e-14 65.1
Msa1224230 AT2G29735.1 33.929 112 65 5 6 113 11 117 2.49e-13 62.0
Msa1224230 AT4G09466.1 28.182 110 76 2 4 113 9 115 3.05e-13 61.6
Msa1224230 AT4G34480.1 32.941 85 56 1 28 112 364 447 3.16e-13 65.1
Msa1224230 AT4G34480.3 32.941 85 56 1 28 112 253 336 4.04e-13 64.7
Msa1224230 AT4G09090.1 28.440 109 75 2 4 112 9 114 5.33e-13 60.8
Msa1224230 AT5G53610.1 32.110 109 69 2 4 112 7 110 6.22e-13 60.5
Msa1224230 AT5G20870.1 34.783 92 54 3 24 112 371 459 6.23e-13 63.9
Msa1224230 AT5G53600.1 35.632 87 52 2 27 112 28 111 1.64e-12 59.7
Msa1224230 AT2G16230.1 32.184 87 58 1 26 112 364 449 6.16e-12 61.2
Msa1224230 AT4G14080.1 28.409 88 63 0 28 115 389 476 6.94e-12 61.2
Msa1224230 AT2G43670.1 31.818 88 58 1 27 114 34 119 1.41e-11 57.4
Msa1224230 AT5G24318.2 35.443 79 50 1 28 106 372 449 1.60e-11 60.1
Msa1224230 AT2G43660.2 32.632 95 63 1 20 114 28 121 5.41e-11 55.8
Msa1224230 AT2G43660.1 32.632 95 63 1 20 114 27 120 6.09e-11 55.8

Find 45 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGGTCCTTCATAGTATT+TGG 0.188691 8_1:-89020446 None:intergenic
ATAGAATGTAGTTGGCATTT+AGG 0.196616 8_1:-89021177 None:intergenic
CATAGTATTTGGAAGGTAAC+AGG 0.246733 8_1:-89020435 None:intergenic
TGAGGAATTCATGGTTCTTC+AGG 0.269762 8_1:-89020078 None:intergenic
TAGAATGTAGTTGGCATTTA+GGG 0.285616 8_1:-89021176 None:intergenic
GCAAATTTCAGGACAATTCA+TGG 0.294807 8_1:+89020304 Msa1224230:intron
ATGTATATTATGTCATTATA+AGG 0.303665 8_1:+89020024 Msa1224230:five_prime_UTR
TATATCCCTTGAATGAAGTT+TGG 0.309961 8_1:+89020979 Msa1224230:exon
TTTGATCATTGAGGAATTCA+TGG 0.314100 8_1:-89020087 None:intergenic
TAAATGCCAACTACATTCTA+TGG 0.342090 8_1:+89021179 Msa1224230:three_prime_UTR
ATATCCCTTGAATGAAGTTT+GGG 0.347148 8_1:+89020980 Msa1224230:exon
GCCCTCTGCAGATCATCATC+TGG 0.401963 8_1:-89020353 None:intergenic
CAAGTGACATAAGGCATTGC+TGG 0.424094 8_1:-89021208 None:intergenic
ATGGATTGGGCTTGTCATGT+TGG 0.425033 8_1:+89020377 Msa1224230:CDS
ATCTGCAGAGGGCTATGGAT+TGG 0.450182 8_1:+89020363 Msa1224230:CDS
ATTACCCCAAACTTCATTCA+AGG 0.455553 8_1:-89020985 None:intergenic
TCTGCAGAGGGCTATGGATT+GGG 0.456199 8_1:+89020364 Msa1224230:CDS
CCCTCTGCAGATCATCATCT+GGG 0.469613 8_1:-89020352 None:intergenic
ACCTGATGCTTGAAAAGGTA+GGG 0.472261 8_1:-89020135 None:intergenic
TGATGATCTGCAGAGGGCTA+TGG 0.487175 8_1:+89020358 Msa1224230:CDS
TTTCAGGACAATTCATGGAA+TGG 0.519046 8_1:+89020309 Msa1224230:intron
GATTGGGCTTGTCATGTTGG+TGG 0.526500 8_1:+89020380 Msa1224230:CDS
TTACCTTCCAAATACTATGA+AGG 0.531309 8_1:+89020439 Msa1224230:CDS
TTTACTTGGATCAAGATCAC+TGG 0.543257 8_1:-89020546 None:intergenic
TATTTGGAAGGTAACAGGGT+TGG 0.547742 8_1:-89020430 None:intergenic
ATAGTAATTGTTGAAGACAT+AGG 0.556410 8_1:-89020471 None:intergenic
TTCACACCATAGAATGTAGT+TGG 0.556550 8_1:-89021185 None:intergenic
CTTGAAAAGGTAGGGTCACA+AGG 0.557030 8_1:-89020127 None:intergenic
GTTGGCATTTAGGGTGGGCA+AGG 0.558771 8_1:-89021167 None:intergenic
TTACCCCAAACTTCATTCAA+GGG 0.566615 8_1:-89020984 None:intergenic
AATGTAGTTGGCATTTAGGG+TGG 0.571985 8_1:-89021173 None:intergenic
GGTCCTTCATAGTATTTGGA+AGG 0.583934 8_1:-89020442 None:intergenic
TGTTGAAGACATAGGAGGCA+TGG 0.588471 8_1:-89020463 None:intergenic
ATGTAGTTGGCATTTAGGGT+GGG 0.589738 8_1:-89021172 None:intergenic
ATAGTATTTGGAAGGTAACA+GGG 0.607638 8_1:-89020434 None:intergenic
TACCTGATGCTTGAAAAGGT+AGG 0.609047 8_1:-89020136 None:intergenic
CAGAAGTTCAAGCACAAAGG+AGG 0.618787 8_1:+89020494 Msa1224230:CDS
GATATGAAGTAGTATTAGCA+TGG 0.620936 8_1:+89021090 Msa1224230:three_prime_UTR
TTATAAGGAATATAGTTGCA+AGG 0.622058 8_1:+89020039 Msa1224230:five_prime_UTR
CCCAGATGATGATCTGCAGA+GGG 0.624946 8_1:+89020352 Msa1224230:CDS
GTAATTGTTGAAGACATAGG+AGG 0.633395 8_1:-89020468 None:intergenic
CCCCAGATGATGATCTGCAG+AGG 0.639650 8_1:+89020351 Msa1224230:CDS
TATCAGAAGTTCAAGCACAA+AGG 0.651785 8_1:+89020491 Msa1224230:CDS
CCTCTGCAGATCATCATCTG+GGG 0.680867 8_1:-89020351 None:intergenic
TATCCCTTGAATGAAGTTTG+GGG 0.706452 8_1:+89020981 Msa1224230:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAAATGAGTTAAATTAA+AGG + chr8_1:89020230-89020249 Msa1224230:intron 10.0%
!!! TATTTTCTCAATTTTTTATT+TGG + chr8_1:89020754-89020773 Msa1224230:intron 10.0%
!!! TTTAAAATGAGTTAAATTAA+AGG + chr8_1:89020230-89020249 Msa1224230:intron 10.0%
!!! TATTTTCTCAATTTTTTATT+TGG + chr8_1:89020754-89020773 Msa1224230:intron 10.0%
!! ATGTATATTATGTCATTATA+AGG + chr8_1:89020024-89020043 Msa1224230:five_prime_UTR 15.0%
!!! TTATTTTTCTGCAATTTAAA+TGG + chr8_1:89021133-89021152 Msa1224230:three_prime_UTR 15.0%
!! ATGTATATTATGTCATTATA+AGG + chr8_1:89020024-89020043 Msa1224230:five_prime_UTR 15.0%
!!! TTATTTTTCTGCAATTTAAA+TGG + chr8_1:89021133-89021152 Msa1224230:three_prime_UTR 15.0%
!!! TATCAAAATTTTGATCATTG+AGG - chr8_1:89020099-89020118 None:intergenic 20.0%
!! GTCATTTAAGTAAAATCAAA+AGG - chr8_1:89020858-89020877 None:intergenic 20.0%
!!! GTTTTTCTTTGTTTAATTAC+AGG + chr8_1:89020934-89020953 Msa1224230:intron 20.0%
!!! TATCAAAATTTTGATCATTG+AGG - chr8_1:89020099-89020118 None:intergenic 20.0%
!! GTCATTTAAGTAAAATCAAA+AGG - chr8_1:89020858-89020877 None:intergenic 20.0%
!!! GTTTTTCTTTGTTTAATTAC+AGG + chr8_1:89020934-89020953 Msa1224230:intron 20.0%
! TTATAAGGAATATAGTTGCA+AGG + chr8_1:89020039-89020058 Msa1224230:five_prime_UTR 25.0%
!! TGATGATGATGATGTATTAT+TGG + chr8_1:89020282-89020301 Msa1224230:intron 25.0%
! ATGTATTATTGGCAAATTTC+AGG + chr8_1:89020293-89020312 Msa1224230:intron 25.0%
! ATAGTAATTGTTGAAGACAT+AGG - chr8_1:89020474-89020493 None:intergenic 25.0%
!!! ATTATGAGAGAGTATTTACT+TGG - chr8_1:89020563-89020582 None:intergenic 25.0%
! TTGACTGTTACTATATGTAT+TGG - chr8_1:89020728-89020747 None:intergenic 25.0%
! TTCATAGATGAATACCATAT+GGG + chr8_1:89020823-89020842 Msa1224230:intron 25.0%
! CAAAGAAAAACCATATCATT+TGG - chr8_1:89020925-89020944 None:intergenic 25.0%
! TTATAAGGAATATAGTTGCA+AGG + chr8_1:89020039-89020058 Msa1224230:five_prime_UTR 25.0%
!! TGATGATGATGATGTATTAT+TGG + chr8_1:89020282-89020301 Msa1224230:intron 25.0%
! ATGTATTATTGGCAAATTTC+AGG + chr8_1:89020293-89020312 Msa1224230:intron 25.0%
! ATAGTAATTGTTGAAGACAT+AGG - chr8_1:89020474-89020493 None:intergenic 25.0%
!!! ATTATGAGAGAGTATTTACT+TGG - chr8_1:89020563-89020582 None:intergenic 25.0%
! TTGACTGTTACTATATGTAT+TGG - chr8_1:89020728-89020747 None:intergenic 25.0%
! TTCATAGATGAATACCATAT+GGG + chr8_1:89020823-89020842 Msa1224230:intron 25.0%
! CAAAGAAAAACCATATCATT+TGG - chr8_1:89020925-89020944 None:intergenic 25.0%
TTTGATCATTGAGGAATTCA+TGG - chr8_1:89020090-89020109 None:intergenic 30.0%
! ATTTTACCTGATGCTTGAAA+AGG - chr8_1:89020143-89020162 None:intergenic 30.0%
ACTTGGAAGAACATAAATGA+GGG - chr8_1:89020174-89020193 None:intergenic 30.0%
AACTTGGAAGAACATAAATG+AGG - chr8_1:89020175-89020194 None:intergenic 30.0%
ATAGTATTTGGAAGGTAACA+GGG - chr8_1:89020437-89020456 None:intergenic 30.0%
TTACCTTCCAAATACTATGA+AGG + chr8_1:89020439-89020458 Msa1224230:CDS 30.0%
GTTCATAGATGAATACCATA+TGG + chr8_1:89020822-89020841 Msa1224230:intron 30.0%
CATTATCAGTCCAAATGATA+TGG + chr8_1:89020912-89020931 Msa1224230:intron 30.0%
! CTTTGTTTAATTACAGGTCA+TGG + chr8_1:89020940-89020959 Msa1224230:intron 30.0%
TATATCCCTTGAATGAAGTT+TGG + chr8_1:89020979-89020998 Msa1224230:exon 30.0%
ATATCCCTTGAATGAAGTTT+GGG + chr8_1:89020980-89020999 Msa1224230:exon 30.0%
GATATGAAGTAGTATTAGCA+TGG + chr8_1:89021090-89021109 Msa1224230:three_prime_UTR 30.0%
TAGAATGTAGTTGGCATTTA+GGG - chr8_1:89021179-89021198 None:intergenic 30.0%
ATAGAATGTAGTTGGCATTT+AGG - chr8_1:89021180-89021199 None:intergenic 30.0%
TAAATGCCAACTACATTCTA+TGG + chr8_1:89021179-89021198 Msa1224230:three_prime_UTR 30.0%
TTTGATCATTGAGGAATTCA+TGG - chr8_1:89020090-89020109 None:intergenic 30.0%
! ATTTTACCTGATGCTTGAAA+AGG - chr8_1:89020143-89020162 None:intergenic 30.0%
ACTTGGAAGAACATAAATGA+GGG - chr8_1:89020174-89020193 None:intergenic 30.0%
AACTTGGAAGAACATAAATG+AGG - chr8_1:89020175-89020194 None:intergenic 30.0%
ATAGTATTTGGAAGGTAACA+GGG - chr8_1:89020437-89020456 None:intergenic 30.0%
TTACCTTCCAAATACTATGA+AGG + chr8_1:89020439-89020458 Msa1224230:CDS 30.0%
GTTCATAGATGAATACCATA+TGG + chr8_1:89020822-89020841 Msa1224230:intron 30.0%
CATTATCAGTCCAAATGATA+TGG + chr8_1:89020912-89020931 Msa1224230:intron 30.0%
! CTTTGTTTAATTACAGGTCA+TGG + chr8_1:89020940-89020959 Msa1224230:intron 30.0%
TATATCCCTTGAATGAAGTT+TGG + chr8_1:89020979-89020998 Msa1224230:exon 30.0%
ATATCCCTTGAATGAAGTTT+GGG + chr8_1:89020980-89020999 Msa1224230:exon 30.0%
GATATGAAGTAGTATTAGCA+TGG + chr8_1:89021090-89021109 Msa1224230:three_prime_UTR 30.0%
TAGAATGTAGTTGGCATTTA+GGG - chr8_1:89021179-89021198 None:intergenic 30.0%
ATAGAATGTAGTTGGCATTT+AGG - chr8_1:89021180-89021199 None:intergenic 30.0%
TAAATGCCAACTACATTCTA+TGG + chr8_1:89021179-89021198 Msa1224230:three_prime_UTR 30.0%
CTAGCTGAATGTGATAAACT+TGG - chr8_1:89020191-89020210 None:intergenic 35.0%
ATTCAGCTAGAGAGTTTACT+TGG + chr8_1:89020201-89020220 Msa1224230:intron 35.0%
GCAAATTTCAGGACAATTCA+TGG + chr8_1:89020304-89020323 Msa1224230:intron 35.0%
TTTCAGGACAATTCATGGAA+TGG + chr8_1:89020309-89020328 Msa1224230:intron 35.0%
CATAGTATTTGGAAGGTAAC+AGG - chr8_1:89020438-89020457 None:intergenic 35.0%
GTAATTGTTGAAGACATAGG+AGG - chr8_1:89020471-89020490 None:intergenic 35.0%
TATCAGAAGTTCAAGCACAA+AGG + chr8_1:89020491-89020510 Msa1224230:CDS 35.0%
TTTACTTGGATCAAGATCAC+TGG - chr8_1:89020549-89020568 None:intergenic 35.0%
!! GAGAAGTATTGGTTCTATAG+AGG - chr8_1:89020653-89020672 None:intergenic 35.0%
TATCCCTTGAATGAAGTTTG+GGG + chr8_1:89020981-89021000 Msa1224230:exon 35.0%
TTACCCCAAACTTCATTCAA+GGG - chr8_1:89020987-89021006 None:intergenic 35.0%
ATTACCCCAAACTTCATTCA+AGG - chr8_1:89020988-89021007 None:intergenic 35.0%
TTCACACCATAGAATGTAGT+TGG - chr8_1:89021188-89021207 None:intergenic 35.0%
CTAGCTGAATGTGATAAACT+TGG - chr8_1:89020191-89020210 None:intergenic 35.0%
ATTCAGCTAGAGAGTTTACT+TGG + chr8_1:89020201-89020220 Msa1224230:intron 35.0%
GCAAATTTCAGGACAATTCA+TGG + chr8_1:89020304-89020323 Msa1224230:intron 35.0%
TTTCAGGACAATTCATGGAA+TGG + chr8_1:89020309-89020328 Msa1224230:intron 35.0%
CATAGTATTTGGAAGGTAAC+AGG - chr8_1:89020438-89020457 None:intergenic 35.0%
GTAATTGTTGAAGACATAGG+AGG - chr8_1:89020471-89020490 None:intergenic 35.0%
TATCAGAAGTTCAAGCACAA+AGG + chr8_1:89020491-89020510 Msa1224230:CDS 35.0%
TTTACTTGGATCAAGATCAC+TGG - chr8_1:89020549-89020568 None:intergenic 35.0%
!! GAGAAGTATTGGTTCTATAG+AGG - chr8_1:89020653-89020672 None:intergenic 35.0%
TATCCCTTGAATGAAGTTTG+GGG + chr8_1:89020981-89021000 Msa1224230:exon 35.0%
TTACCCCAAACTTCATTCAA+GGG - chr8_1:89020987-89021006 None:intergenic 35.0%
ATTACCCCAAACTTCATTCA+AGG - chr8_1:89020988-89021007 None:intergenic 35.0%
TTCACACCATAGAATGTAGT+TGG - chr8_1:89021188-89021207 None:intergenic 35.0%
TGAGGAATTCATGGTTCTTC+AGG - chr8_1:89020081-89020100 None:intergenic 40.0%
ACCTGATGCTTGAAAAGGTA+GGG - chr8_1:89020138-89020157 None:intergenic 40.0%
TACCTGATGCTTGAAAAGGT+AGG - chr8_1:89020139-89020158 None:intergenic 40.0%
TATTTGGAAGGTAACAGGGT+TGG - chr8_1:89020433-89020452 None:intergenic 40.0%
GGTCCTTCATAGTATTTGGA+AGG - chr8_1:89020445-89020464 None:intergenic 40.0%
GCATGGTCCTTCATAGTATT+TGG - chr8_1:89020449-89020468 None:intergenic 40.0%
AGTGTGACACTAACACGTAT+TGG - chr8_1:89020696-89020715 None:intergenic 40.0%
! AAAGGCTTTTGTGTCCCATA+TGG - chr8_1:89020840-89020859 None:intergenic 40.0%
! ATGTAGTTGGCATTTAGGGT+GGG - chr8_1:89021175-89021194 None:intergenic 40.0%
AATGTAGTTGGCATTTAGGG+TGG - chr8_1:89021176-89021195 None:intergenic 40.0%
TGAGGAATTCATGGTTCTTC+AGG - chr8_1:89020081-89020100 None:intergenic 40.0%
ACCTGATGCTTGAAAAGGTA+GGG - chr8_1:89020138-89020157 None:intergenic 40.0%
TACCTGATGCTTGAAAAGGT+AGG - chr8_1:89020139-89020158 None:intergenic 40.0%
TATTTGGAAGGTAACAGGGT+TGG - chr8_1:89020433-89020452 None:intergenic 40.0%
GGTCCTTCATAGTATTTGGA+AGG - chr8_1:89020445-89020464 None:intergenic 40.0%
GCATGGTCCTTCATAGTATT+TGG - chr8_1:89020449-89020468 None:intergenic 40.0%
AGTGTGACACTAACACGTAT+TGG - chr8_1:89020696-89020715 None:intergenic 40.0%
! AAAGGCTTTTGTGTCCCATA+TGG - chr8_1:89020840-89020859 None:intergenic 40.0%
! ATGTAGTTGGCATTTAGGGT+GGG - chr8_1:89021175-89021194 None:intergenic 40.0%
AATGTAGTTGGCATTTAGGG+TGG - chr8_1:89021176-89021195 None:intergenic 40.0%
CTTGAAAAGGTAGGGTCACA+AGG - chr8_1:89020130-89020149 None:intergenic 45.0%
!! ACCCTACCTTTTCAAGCATC+AGG + chr8_1:89020134-89020153 Msa1224230:CDS 45.0%
!! ATGGATTGGGCTTGTCATGT+TGG + chr8_1:89020377-89020396 Msa1224230:CDS 45.0%
TGTTGAAGACATAGGAGGCA+TGG - chr8_1:89020466-89020485 None:intergenic 45.0%
CAGAAGTTCAAGCACAAAGG+AGG + chr8_1:89020494-89020513 Msa1224230:CDS 45.0%
! ACGTCTTCGAGGAGAAGTAT+TGG - chr8_1:89020664-89020683 None:intergenic 45.0%
CTTACACAGACACCGACAAA+GGG - chr8_1:89020801-89020820 None:intergenic 45.0%
ACTTACACAGACACCGACAA+AGG - chr8_1:89020802-89020821 None:intergenic 45.0%
CTTGAAAAGGTAGGGTCACA+AGG - chr8_1:89020130-89020149 None:intergenic 45.0%
!! ACCCTACCTTTTCAAGCATC+AGG + chr8_1:89020134-89020153 Msa1224230:CDS 45.0%
!! ATGGATTGGGCTTGTCATGT+TGG + chr8_1:89020377-89020396 Msa1224230:CDS 45.0%
TGTTGAAGACATAGGAGGCA+TGG - chr8_1:89020466-89020485 None:intergenic 45.0%
CAGAAGTTCAAGCACAAAGG+AGG + chr8_1:89020494-89020513 Msa1224230:CDS 45.0%
! ACGTCTTCGAGGAGAAGTAT+TGG - chr8_1:89020664-89020683 None:intergenic 45.0%
CTTACACAGACACCGACAAA+GGG - chr8_1:89020801-89020820 None:intergenic 45.0%
ACTTACACAGACACCGACAA+AGG - chr8_1:89020802-89020821 None:intergenic 45.0%
CCTCTGCAGATCATCATCTG+GGG - chr8_1:89020354-89020373 None:intergenic 50.0%
CCCTCTGCAGATCATCATCT+GGG - chr8_1:89020355-89020374 None:intergenic 50.0%
CCCAGATGATGATCTGCAGA+GGG + chr8_1:89020352-89020371 Msa1224230:CDS 50.0%
TGATGATCTGCAGAGGGCTA+TGG + chr8_1:89020358-89020377 Msa1224230:CDS 50.0%
ATCTGCAGAGGGCTATGGAT+TGG + chr8_1:89020363-89020382 Msa1224230:CDS 50.0%
TCTGCAGAGGGCTATGGATT+GGG + chr8_1:89020364-89020383 Msa1224230:CDS 50.0%
GATTGGGCTTGTCATGTTGG+TGG + chr8_1:89020380-89020399 Msa1224230:CDS 50.0%
GTCAGTATCACGCCCTTTGT+CGG + chr8_1:89020786-89020805 Msa1224230:intron 50.0%
CCTCTGCAGATCATCATCTG+GGG - chr8_1:89020354-89020373 None:intergenic 50.0%
CCCTCTGCAGATCATCATCT+GGG - chr8_1:89020355-89020374 None:intergenic 50.0%
CCCAGATGATGATCTGCAGA+GGG + chr8_1:89020352-89020371 Msa1224230:CDS 50.0%
TGATGATCTGCAGAGGGCTA+TGG + chr8_1:89020358-89020377 Msa1224230:CDS 50.0%
ATCTGCAGAGGGCTATGGAT+TGG + chr8_1:89020363-89020382 Msa1224230:CDS 50.0%
TCTGCAGAGGGCTATGGATT+GGG + chr8_1:89020364-89020383 Msa1224230:CDS 50.0%
GATTGGGCTTGTCATGTTGG+TGG + chr8_1:89020380-89020399 Msa1224230:CDS 50.0%
GTCAGTATCACGCCCTTTGT+CGG + chr8_1:89020786-89020805 Msa1224230:intron 50.0%
CCCCAGATGATGATCTGCAG+AGG + chr8_1:89020351-89020370 Msa1224230:CDS 55.0%
GCCCTCTGCAGATCATCATC+TGG - chr8_1:89020356-89020375 None:intergenic 55.0%
GGACACTGAACACGTCTTCG+AGG - chr8_1:89020675-89020694 None:intergenic 55.0%
! GTTGGCATTTAGGGTGGGCA+AGG - chr8_1:89021170-89021189 None:intergenic 55.0%
CCCCAGATGATGATCTGCAG+AGG + chr8_1:89020351-89020370 Msa1224230:CDS 55.0%
GCCCTCTGCAGATCATCATC+TGG - chr8_1:89020356-89020375 None:intergenic 55.0%
GGACACTGAACACGTCTTCG+AGG - chr8_1:89020675-89020694 None:intergenic 55.0%
! GTTGGCATTTAGGGTGGGCA+AGG - chr8_1:89021170-89021189 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 89020000 89021223 89020000 ID=Msa1224230;Name=Msa1224230
chr8_1 mRNA 89020000 89021223 89020000 ID=Msa1224230-mRNA-1;Parent=Msa1224230;Name=Msa1224230-mRNA-1;_AED=0.25;_eAED=0.25;_QI=89|1|1|1|1|1|3|233|116
chr8_1 exon 89020000 89020155 89020000 ID=Msa1224230-mRNA-1:exon:32358;Parent=Msa1224230-mRNA-1
chr8_1 exon 89020315 89020563 89020315 ID=Msa1224230-mRNA-1:exon:32359;Parent=Msa1224230-mRNA-1
chr8_1 exon 89020956 89021223 89020956 ID=Msa1224230-mRNA-1:exon:32360;Parent=Msa1224230-mRNA-1
chr8_1 five_prime_UTR 89020000 89020088 89020000 ID=Msa1224230-mRNA-1:five_prime_utr;Parent=Msa1224230-mRNA-1
chr8_1 CDS 89020089 89020155 89020089 ID=Msa1224230-mRNA-1:cds;Parent=Msa1224230-mRNA-1
chr8_1 CDS 89020315 89020563 89020315 ID=Msa1224230-mRNA-1:cds;Parent=Msa1224230-mRNA-1
chr8_1 CDS 89020956 89020990 89020956 ID=Msa1224230-mRNA-1:cds;Parent=Msa1224230-mRNA-1
chr8_1 three_prime_UTR 89020991 89021223 89020991 ID=Msa1224230-mRNA-1:three_prime_utr;Parent=Msa1224230-mRNA-1
Gene Sequence

>Msa1224230

ATGAATTCCTCAATGATCAAAATTTTGATAGCTATGCTCCTTGTGACCCTACCTTTTCAAGCATCAGGACAATTCATGGAATGGTGCATAGCTGATGAGCAGACCCCAGATGATGATCTGCAGAGGGCTATGGATTGGGCTTGTCATGTTGGTGGAGCAAATTGTAGCAACATACAAGTGAACCAACCCTGTTACCTTCCAAATACTATGAAGGACCATGCCTCCTATGTCTTCAACAATTACTATCAGAAGTTCAAGCACAAAGGAGGATCTTGCTATTTTAACTCTGCTGCAATAACCAGTGATCTTGATCCAAGTCATGGCTCTTGCAAGTTTGAGTATATCCCTTGA
Protein sequence

>Msa1224230

MNSSMIKILIAMLLVTLPFQASGQFMEWCIADEQTPDDDLQRAMDWACHVGGANCSNIQVNQPCYLPNTMKDHASYVFNNYYQKFKHKGGSCYFNSAAITSDLDPSHGSCKFEYIP*