AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0746120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0746120 MtrunA17_Chr4g0076131 95.690 116 5 0 1 116 1 116 1.07e-83 239
Msa0746120 MtrunA17_Chr4g0076141 72.165 97 27 0 19 115 4 100 7.81e-49 150
Msa0746120 MtrunA17_Chr5g0447651 55.932 118 50 1 1 116 1 118 1.00e-48 151
Msa0746120 MtrunA17_Chr8g0378621 50.588 85 42 0 28 112 364 448 1.06e-25 99.8
Msa0746120 MtrunA17_Chr3g0121271 45.349 86 47 0 27 112 361 446 1.38e-24 96.7
Msa0746120 MtrunA17_Chr4g0075031 51.765 85 41 0 28 112 382 466 2.47e-24 96.3
Msa0746120 MtrunA17_Chr4g0040911 47.059 85 45 0 28 112 37 121 4.04e-23 88.2
Msa0746120 MtrunA17_Chr1g0213651 44.828 87 47 1 28 114 276 361 2.03e-22 89.7
Msa0746120 MtrunA17_Chr8g0378361 38.095 105 64 1 8 112 10 113 3.20e-22 86.3
Msa0746120 MtrunA17_Chr3g0120401 44.444 90 49 1 24 113 153 241 3.77e-22 87.4
Msa0746120 MtrunA17_Chr1g0177171 44.706 85 47 0 28 112 406 490 1.69e-21 88.2
Msa0746120 MtrunA17_Chr8g0389741 42.353 85 49 0 28 112 368 452 3.92e-21 87.0
Msa0746120 MtrunA17_Chr5g0401301 47.674 86 44 1 28 112 329 414 7.24e-21 85.9
Msa0746120 MtrunA17_Chr2g0304061 48.276 87 43 2 27 113 157 241 9.08e-21 85.1
Msa0746120 MtrunA17_Chr8g0381081 38.384 99 58 1 18 113 44 142 1.15e-20 82.0
Msa0746120 MtrunA17_Chr3g0096451 44.186 86 47 1 27 112 111 195 1.54e-19 80.5
Msa0746120 MtrunA17_Chr5g0439251 46.835 79 42 0 28 106 382 460 1.57e-19 82.4
Msa0746120 MtrunA17_Chr7g0234821 41.176 85 50 0 28 112 374 458 1.80e-19 82.4
Msa0746120 MtrunA17_Chr7g0271411 41.860 86 49 1 28 113 160 244 4.07e-19 80.1
Msa0746120 MtrunA17_Chr1g0161011 42.353 85 49 0 28 112 361 445 4.30e-19 81.3
Msa0746120 MtrunA17_Chr8g0378371 36.364 110 66 2 3 112 8 113 1.55e-18 74.7
Msa0746120 MtrunA17_Chr8g0339191 37.647 85 53 0 28 112 374 458 3.31e-18 78.6
Msa0746120 MtrunA17_Chr3g0110371 42.353 85 49 0 28 112 361 445 6.63e-18 77.8
Msa0746120 MtrunA17_Chr5g0443331 41.860 86 49 1 27 112 107 191 7.14e-18 76.3
Msa0746120 MtrunA17_Chr6g0487261 39.080 87 52 1 27 112 368 454 7.76e-18 77.8
Msa0746120 MtrunA17_Chr1g0193131 45.977 87 45 2 22 108 414 498 7.91e-18 77.8
Msa0746120 MtrunA17_Chr5g0434591 41.176 85 50 0 28 112 360 444 8.10e-18 77.8
Msa0746120 MtrunA17_Chr5g0419121 36.893 103 62 2 12 114 8 107 8.41e-18 75.9
Msa0746120 MtrunA17_Chr8g0378381 34.259 108 67 2 5 112 12 115 9.96e-18 72.8
Msa0746120 MtrunA17_Chr4g0043151 41.463 82 47 1 28 109 21 101 2.78e-16 70.9
Msa0746120 MtrunA17_Chr4g0061401 37.895 95 57 2 18 112 14 106 3.85e-16 70.9
Msa0746120 MtrunA17_Chr8g0378401 33.333 111 70 2 5 115 12 118 4.33e-16 68.6
Msa0746120 MtrunA17_Chr4g0043511 35.227 88 56 1 27 114 419 505 1.08e-15 71.6
Msa0746120 MtrunA17_Chr8g0380231 48.611 72 36 1 35 106 24 94 1.15e-15 67.0
Msa0746120 MtrunA17_Chr4g0046311 39.241 79 48 0 28 106 373 451 1.97e-15 70.9
Msa0746120 MtrunA17_Chr4g0034901 37.209 86 54 0 27 112 374 459 2.53e-15 70.5
Msa0746120 MtrunA17_Chr1g0154991 31.959 97 62 2 16 112 11 103 3.47e-12 58.2
Msa0746120 MtrunA17_Chr3g0144531 34.118 85 55 1 28 112 371 454 1.01e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0746120 AT5G35740.1 63.107 103 37 1 15 116 17 119 1.24e-48 151
Msa0746120 AT2G04910.2 63.000 100 37 0 17 116 4 103 7.67e-46 144
Msa0746120 AT2G04910.1 62.222 90 34 0 17 106 4 93 2.26e-39 128
Msa0746120 AT3G13560.4 50.588 85 42 0 28 112 362 446 6.57e-26 100
Msa0746120 AT3G13560.1 50.588 85 42 0 28 112 362 446 6.57e-26 100
Msa0746120 AT3G13560.2 50.588 85 42 0 28 112 362 446 6.57e-26 100
Msa0746120 AT3G13560.5 50.588 85 42 0 28 112 362 446 6.57e-26 100
Msa0746120 AT3G13560.3 50.588 85 42 0 28 112 362 446 6.57e-26 100
Msa0746120 AT3G58100.1 41.071 112 63 1 4 112 14 125 1.06e-25 95.1
Msa0746120 AT3G58100.2 41.071 112 63 1 4 112 90 201 8.18e-25 94.7
Msa0746120 AT4G29360.1 43.137 102 58 0 15 116 378 479 5.29e-24 95.5
Msa0746120 AT4G29360.2 43.137 102 58 0 15 116 388 489 5.43e-24 95.5
Msa0746120 AT1G11820.2 45.631 103 49 1 17 112 363 465 6.68e-24 95.1
Msa0746120 AT1G29380.1 51.724 87 39 2 27 112 147 231 1.72e-23 92.4
Msa0746120 AT1G29380.3 51.136 88 40 2 27 113 147 232 1.76e-23 91.3
Msa0746120 AT2G05790.1 45.882 85 46 0 28 112 384 468 2.16e-23 93.6
Msa0746120 AT2G30933.1 43.925 107 55 2 10 112 60 165 4.05e-23 89.7
Msa0746120 AT4G05430.1 39.175 97 56 1 20 113 12 108 9.09e-23 86.7
Msa0746120 AT4G05430.3 39.175 97 56 1 20 113 37 133 1.76e-22 86.7
Msa0746120 AT1G79480.1 43.678 87 48 1 28 114 311 396 3.81e-22 89.7
Msa0746120 AT1G79480.2 43.678 87 48 1 28 114 310 395 4.02e-22 89.7
Msa0746120 AT5G67460.1 44.706 85 47 0 28 112 293 377 5.48e-22 89.4
Msa0746120 AT4G26830.2 42.353 85 49 0 28 112 371 455 9.33e-22 89.0
Msa0746120 AT4G26830.1 42.353 85 49 0 28 112 367 451 9.39e-22 89.0
Msa0746120 AT5G55180.1 40.625 96 57 0 17 112 361 456 2.01e-21 88.2
Msa0746120 AT5G56590.1 41.176 85 50 0 28 112 369 453 6.88e-21 86.7
Msa0746120 AT4G13600.1 44.706 85 46 1 28 112 61 144 7.98e-21 84.0
Msa0746120 AT1G29380.4 50.000 84 39 2 27 109 147 228 1.38e-20 83.2
Msa0746120 AT1G29380.2 50.000 84 39 2 27 109 147 228 1.38e-20 83.2
Msa0746120 AT2G30933.2 42.574 101 53 2 10 106 60 159 2.19e-20 81.3
Msa0746120 AT2G30933.3 42.574 101 53 2 10 106 60 159 2.19e-20 81.3
Msa0746120 AT5G63240.1 40.000 95 55 1 20 112 32 126 2.81e-20 80.1
Msa0746120 AT5G63250.1 41.379 87 49 1 28 112 42 128 7.78e-20 79.0
Msa0746120 AT5G67460.2 45.570 79 43 0 28 106 293 371 8.69e-20 83.2
Msa0746120 AT4G05430.2 40.000 90 51 1 20 106 37 126 9.29e-20 78.6
Msa0746120 AT1G11820.3 43.299 97 48 1 17 106 363 459 1.29e-19 83.2
Msa0746120 AT1G18650.2 36.111 108 65 2 5 112 1 104 2.05e-19 77.8
Msa0746120 AT1G66855.1 34.821 112 71 1 1 112 1 110 2.17e-19 77.0
Msa0746120 AT1G18650.1 36.111 108 65 2 5 112 1 104 3.75e-19 78.6
Msa0746120 AT2G01630.1 42.353 85 49 0 28 112 360 444 5.96e-19 81.3
Msa0746120 AT5G55180.2 41.111 90 53 0 17 106 361 450 7.12e-19 80.9
Msa0746120 AT1G66870.2 32.710 107 70 1 6 112 33 137 5.24e-18 74.3
Msa0746120 AT1G66870.1 31.250 112 75 1 1 112 1 110 5.80e-18 73.6
Msa0746120 AT1G66250.1 40.000 85 51 0 28 112 368 452 1.42e-17 77.4
Msa0746120 AT1G66852.1 33.333 105 67 2 8 112 8 109 1.51e-16 70.1
Msa0746120 AT2G01630.3 41.772 79 46 0 28 106 360 438 4.48e-16 73.2
Msa0746120 AT4G16165.1 30.973 113 76 1 1 113 1 111 4.66e-16 68.6
Msa0746120 AT3G07320.1 37.647 85 53 0 28 112 371 455 6.83e-16 72.4
Msa0746120 AT1G26450.1 37.234 94 55 2 6 99 1 90 9.14e-16 70.1
Msa0746120 AT5G63230.1 40.698 86 50 1 27 112 25 109 1.45e-15 67.4
Msa0746120 AT3G55430.1 32.632 95 60 2 18 112 359 449 2.68e-15 70.9
Msa0746120 AT1G69295.1 37.500 104 61 3 6 109 1 100 5.47e-15 68.6
Msa0746120 AT5G08000.2 31.776 107 72 1 6 112 18 123 6.78e-15 67.8
Msa0746120 AT1G69295.2 41.176 85 48 2 25 109 14 96 7.77e-15 67.8
Msa0746120 AT1G09460.1 42.391 92 48 3 22 113 133 219 7.99e-15 68.9
Msa0746120 AT3G23770.1 32.955 88 59 0 28 115 387 474 1.00e-14 69.3
Msa0746120 AT5G24318.1 34.884 86 55 1 28 113 372 456 1.14e-14 68.9
Msa0746120 AT4G09462.1 30.476 105 72 1 9 113 10 113 1.15e-14 65.1
Msa0746120 AT5G63225.1 36.735 98 58 2 15 112 15 108 1.59e-14 64.7
Msa0746120 AT4G09465.1 29.091 110 75 2 4 113 9 115 2.38e-14 64.3
Msa0746120 AT4G09464.1 29.091 110 75 2 4 113 9 115 3.18e-14 63.9
Msa0746120 AT4G09467.1 29.091 110 75 2 4 113 9 115 3.18e-14 63.9
Msa0746120 AT2G03505.3 39.241 79 47 1 28 106 21 98 5.53e-14 63.5
Msa0746120 AT5G24318.3 34.483 87 56 1 27 113 232 317 5.99e-14 66.6
Msa0746120 AT2G03505.4 39.241 79 47 1 28 106 21 98 7.66e-14 64.3
Msa0746120 AT2G03505.1 39.241 79 47 1 28 106 21 98 7.66e-14 64.3
Msa0746120 AT2G03505.2 39.241 79 47 1 28 106 21 98 7.66e-14 64.3
Msa0746120 AT5G61130.1 33.721 86 56 1 27 112 20 104 7.70e-14 65.1
Msa0746120 AT2G29735.1 33.929 112 65 5 6 113 11 117 2.49e-13 62.0
Msa0746120 AT4G09466.1 28.182 110 76 2 4 113 9 115 3.05e-13 61.6
Msa0746120 AT4G34480.1 32.941 85 56 1 28 112 364 447 3.16e-13 65.1
Msa0746120 AT4G34480.3 32.941 85 56 1 28 112 253 336 4.04e-13 64.7
Msa0746120 AT4G09090.1 28.440 109 75 2 4 112 9 114 5.33e-13 60.8
Msa0746120 AT5G53610.1 32.110 109 69 2 4 112 7 110 6.22e-13 60.5
Msa0746120 AT5G20870.1 34.783 92 54 3 24 112 371 459 6.23e-13 63.9
Msa0746120 AT5G53600.1 35.632 87 52 2 27 112 28 111 1.64e-12 59.7
Msa0746120 AT2G16230.1 32.184 87 58 1 26 112 364 449 6.16e-12 61.2
Msa0746120 AT4G14080.1 28.409 88 63 0 28 115 389 476 6.94e-12 61.2
Msa0746120 AT2G43670.1 31.818 88 58 1 27 114 34 119 1.41e-11 57.4
Msa0746120 AT5G24318.2 35.443 79 50 1 28 106 372 449 1.60e-11 60.1
Msa0746120 AT2G43660.2 32.632 95 63 1 20 114 28 121 5.41e-11 55.8
Msa0746120 AT2G43660.1 32.632 95 63 1 20 114 27 120 6.09e-11 55.8

Find 45 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGGTCCTTCATAGTATT+TGG 0.188691 5_1:+68127127 None:intergenic
ATAGAATGTAGTTGGCATTT+AGG 0.196616 5_1:+68126397 None:intergenic
CATAGTATTTGGAAGGTAAC+AGG 0.246733 5_1:+68127138 None:intergenic
TGAGGAATTCATGGTTCTTC+AGG 0.269762 5_1:+68127495 None:intergenic
TAGAATGTAGTTGGCATTTA+GGG 0.285616 5_1:+68126398 None:intergenic
GAAAATTTCAGGACAATTCA+TGG 0.287986 5_1:-68127269 Msa0746120:intron
ATGTATATTATGTCATTATA+AGG 0.303665 5_1:-68127549 Msa0746120:five_prime_UTR
TATATCCCTTGAATGAAGTT+TGG 0.309961 5_1:-68126595 Msa0746120:exon
TTTGATCATTGAGGAATTCA+TGG 0.314100 5_1:+68127486 None:intergenic
TAAATGCCAACTACATTCTA+TGG 0.342090 5_1:-68126395 Msa0746120:three_prime_UTR
ATATCCCTTGAATGAAGTTT+GGG 0.347148 5_1:-68126594 Msa0746120:exon
GCCCTCTGCAGATCATCATC+TGG 0.401963 5_1:+68127220 None:intergenic
CAAGTGACATAAGGCATTGC+TGG 0.424094 5_1:+68126366 None:intergenic
ATGGATTGGGCTTGTCATGT+TGG 0.425033 5_1:-68127196 Msa0746120:CDS
ATCTGCAGAGGGCTATGGAT+TGG 0.450182 5_1:-68127210 Msa0746120:CDS
ATTACCCCAAACTTCATTCA+AGG 0.455553 5_1:+68126589 None:intergenic
TCTGCAGAGGGCTATGGATT+GGG 0.456199 5_1:-68127209 Msa0746120:CDS
CCCTCTGCAGATCATCATCT+GGG 0.469613 5_1:+68127221 None:intergenic
ACCTGATGCTTGAAAAGGTA+GGG 0.472261 5_1:+68127438 None:intergenic
TGATGATCTGCAGAGGGCTA+TGG 0.487175 5_1:-68127215 Msa0746120:CDS
GTTGGCATTTAGGGTGGGAA+AGG 0.508942 5_1:+68126407 None:intergenic
TTTCAGGACAATTCATGGAA+TGG 0.512225 5_1:-68127264 Msa0746120:intron
AATGTAGTTGGCATTTAGGG+TGG 0.525675 5_1:+68126401 None:intergenic
GATTGGGCTTGTCATGTTGG+TGG 0.526500 5_1:-68127193 Msa0746120:CDS
TTACCTTCCAAATACTATGA+AGG 0.531309 5_1:-68127134 Msa0746120:CDS
TTTACTTGGATCAAGATCAC+TGG 0.543257 5_1:+68127027 None:intergenic
TATTTGGAAGGTAACAGGGT+TGG 0.547742 5_1:+68127143 None:intergenic
ATAGTAATTGTTGAAGACAT+AGG 0.556410 5_1:+68127102 None:intergenic
TTCACACCATAGAATGTAGT+TGG 0.556550 5_1:+68126389 None:intergenic
CTTGAAAAGGTAGGGTCACA+AGG 0.557030 5_1:+68127446 None:intergenic
TTACCCCAAACTTCATTCAA+GGG 0.566615 5_1:+68126590 None:intergenic
ATGTAGTTGGCATTTAGGGT+GGG 0.571532 5_1:+68126402 None:intergenic
GGTCCTTCATAGTATTTGGA+AGG 0.583934 5_1:+68127131 None:intergenic
TGTTGAAGACATAGGAGGCA+TGG 0.588471 5_1:+68127110 None:intergenic
ATAGTATTTGGAAGGTAACA+GGG 0.607638 5_1:+68127139 None:intergenic
TACCTGATGCTTGAAAAGGT+AGG 0.609047 5_1:+68127437 None:intergenic
CAGAAGTTCAAGCACAAAGG+AGG 0.618787 5_1:-68127079 Msa0746120:CDS
GATATGAAGTAGTATTAGCA+TGG 0.620936 5_1:-68126484 Msa0746120:three_prime_UTR
TTATAAGGAATATAGTTGCA+AGG 0.622058 5_1:-68127534 Msa0746120:five_prime_UTR
CCCAGATGATGATCTGCAGA+GGG 0.624946 5_1:-68127221 Msa0746120:CDS
GTAATTGTTGAAGACATAGG+AGG 0.633395 5_1:+68127105 None:intergenic
CCCCAGATGATGATCTGCAG+AGG 0.639650 5_1:-68127222 Msa0746120:CDS
TATCAGAAGTTCAAGCACAA+AGG 0.651785 5_1:-68127082 Msa0746120:CDS
CCTCTGCAGATCATCATCTG+GGG 0.680867 5_1:+68127222 None:intergenic
TATCCCTTGAATGAAGTTTG+GGG 0.706452 5_1:-68126593 Msa0746120:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAAATGAGTTAAATTAA+AGG - chr5_1:68126603-68126622 Msa0746120:exon 10.0%
!!! TATTTTCTCAATTTTTTATT+TGG - chr5_1:68127126-68127145 Msa0746120:CDS 10.0%
!!! TTTAAAATGAGTTAAATTAA+AGG - chr5_1:68126603-68126622 Msa0746120:exon 10.0%
!!! TATTTTCTCAATTTTTTATT+TGG - chr5_1:68127126-68127145 Msa0746120:CDS 10.0%
!! ATGTATATTATGTCATTATA+AGG - chr5_1:68126397-68126416 Msa0746120:three_prime_UTR 15.0%
!!! TTATTTTTCTGCAATTTAAA+TGG - chr5_1:68127505-68127524 Msa0746120:exon 15.0%
!! ATGTATATTATGTCATTATA+AGG - chr5_1:68126397-68126416 Msa0746120:three_prime_UTR 15.0%
!!! TTATTTTTCTGCAATTTAAA+TGG - chr5_1:68127505-68127524 Msa0746120:exon 15.0%
!!! TATCAAAATTTTGATCATTG+AGG + chr5_1:68126472-68126491 None:intergenic 20.0%
!! ATGTATTATTGGAAAATTTC+AGG - chr5_1:68126666-68126685 Msa0746120:intron 20.0%
!! GTCATTTAAGTAAAATCAAA+AGG + chr5_1:68127230-68127249 None:intergenic 20.0%
!!! GTTTTTCTTTGTTTAATTAC+AGG - chr5_1:68127306-68127325 Msa0746120:intron 20.0%
!!! TATCAAAATTTTGATCATTG+AGG + chr5_1:68126472-68126491 None:intergenic 20.0%
!! ATGTATTATTGGAAAATTTC+AGG - chr5_1:68126666-68126685 Msa0746120:intron 20.0%
!! GTCATTTAAGTAAAATCAAA+AGG + chr5_1:68127230-68127249 None:intergenic 20.0%
!!! GTTTTTCTTTGTTTAATTAC+AGG - chr5_1:68127306-68127325 Msa0746120:intron 20.0%
! TTATAAGGAATATAGTTGCA+AGG - chr5_1:68126412-68126431 Msa0746120:three_prime_UTR 25.0%
!! TGATGATGATGATGTATTAT+TGG - chr5_1:68126655-68126674 Msa0746120:intron 25.0%
! ATAGTAATTGTTGAAGACAT+AGG + chr5_1:68126847-68126866 None:intergenic 25.0%
!!! ATTATGAGAGAGTATTTACT+TGG + chr5_1:68126936-68126955 None:intergenic 25.0%
! TTGACTGTTACTATATGTAT+TGG + chr5_1:68127100-68127119 None:intergenic 25.0%
! TTCATAGATGAATACCATAT+GGG - chr5_1:68127195-68127214 Msa0746120:CDS 25.0%
! CAAAGAAAAACCATATCATT+TGG + chr5_1:68127297-68127316 None:intergenic 25.0%
! TTATAAGGAATATAGTTGCA+AGG - chr5_1:68126412-68126431 Msa0746120:three_prime_UTR 25.0%
!! TGATGATGATGATGTATTAT+TGG - chr5_1:68126655-68126674 Msa0746120:intron 25.0%
! ATAGTAATTGTTGAAGACAT+AGG + chr5_1:68126847-68126866 None:intergenic 25.0%
!!! ATTATGAGAGAGTATTTACT+TGG + chr5_1:68126936-68126955 None:intergenic 25.0%
! TTGACTGTTACTATATGTAT+TGG + chr5_1:68127100-68127119 None:intergenic 25.0%
! TTCATAGATGAATACCATAT+GGG - chr5_1:68127195-68127214 Msa0746120:CDS 25.0%
! CAAAGAAAAACCATATCATT+TGG + chr5_1:68127297-68127316 None:intergenic 25.0%
TTTGATCATTGAGGAATTCA+TGG + chr5_1:68126463-68126482 None:intergenic 30.0%
! ATTTTACCTGATGCTTGAAA+AGG + chr5_1:68126516-68126535 None:intergenic 30.0%
ACTTGGAAGAACATAAATGA+GGG + chr5_1:68126547-68126566 None:intergenic 30.0%
AACTTGGAAGAACATAAATG+AGG + chr5_1:68126548-68126567 None:intergenic 30.0%
GAAAATTTCAGGACAATTCA+TGG - chr5_1:68126677-68126696 Msa0746120:intron 30.0%
ATAGTATTTGGAAGGTAACA+GGG + chr5_1:68126810-68126829 None:intergenic 30.0%
TTACCTTCCAAATACTATGA+AGG - chr5_1:68126812-68126831 Msa0746120:intron 30.0%
GTTCATAGATGAATACCATA+TGG - chr5_1:68127194-68127213 Msa0746120:CDS 30.0%
CATTATCAGTCCAAATGATA+TGG - chr5_1:68127284-68127303 Msa0746120:intron 30.0%
! CTTTGTTTAATTACAGGTCA+TGG - chr5_1:68127312-68127331 Msa0746120:intron 30.0%
TATATCCCTTGAATGAAGTT+TGG - chr5_1:68127351-68127370 Msa0746120:intron 30.0%
ATATCCCTTGAATGAAGTTT+GGG - chr5_1:68127352-68127371 Msa0746120:intron 30.0%
GATATGAAGTAGTATTAGCA+TGG - chr5_1:68127462-68127481 Msa0746120:CDS 30.0%
TAGAATGTAGTTGGCATTTA+GGG + chr5_1:68127551-68127570 None:intergenic 30.0%
ATAGAATGTAGTTGGCATTT+AGG + chr5_1:68127552-68127571 None:intergenic 30.0%
TAAATGCCAACTACATTCTA+TGG - chr5_1:68127551-68127570 Msa0746120:five_prime_UTR 30.0%
TTTGATCATTGAGGAATTCA+TGG + chr5_1:68126463-68126482 None:intergenic 30.0%
! ATTTTACCTGATGCTTGAAA+AGG + chr5_1:68126516-68126535 None:intergenic 30.0%
ACTTGGAAGAACATAAATGA+GGG + chr5_1:68126547-68126566 None:intergenic 30.0%
AACTTGGAAGAACATAAATG+AGG + chr5_1:68126548-68126567 None:intergenic 30.0%
GAAAATTTCAGGACAATTCA+TGG - chr5_1:68126677-68126696 Msa0746120:intron 30.0%
ATAGTATTTGGAAGGTAACA+GGG + chr5_1:68126810-68126829 None:intergenic 30.0%
TTACCTTCCAAATACTATGA+AGG - chr5_1:68126812-68126831 Msa0746120:intron 30.0%
GTTCATAGATGAATACCATA+TGG - chr5_1:68127194-68127213 Msa0746120:CDS 30.0%
CATTATCAGTCCAAATGATA+TGG - chr5_1:68127284-68127303 Msa0746120:intron 30.0%
! CTTTGTTTAATTACAGGTCA+TGG - chr5_1:68127312-68127331 Msa0746120:intron 30.0%
TATATCCCTTGAATGAAGTT+TGG - chr5_1:68127351-68127370 Msa0746120:intron 30.0%
ATATCCCTTGAATGAAGTTT+GGG - chr5_1:68127352-68127371 Msa0746120:intron 30.0%
GATATGAAGTAGTATTAGCA+TGG - chr5_1:68127462-68127481 Msa0746120:CDS 30.0%
TAGAATGTAGTTGGCATTTA+GGG + chr5_1:68127551-68127570 None:intergenic 30.0%
ATAGAATGTAGTTGGCATTT+AGG + chr5_1:68127552-68127571 None:intergenic 30.0%
TAAATGCCAACTACATTCTA+TGG - chr5_1:68127551-68127570 Msa0746120:five_prime_UTR 30.0%
CTAGCTGAATGTGATAAACT+TGG + chr5_1:68126564-68126583 None:intergenic 35.0%
ATTCAGCTAGAGAGTTTACT+TGG - chr5_1:68126574-68126593 Msa0746120:three_prime_UTR 35.0%
TTTCAGGACAATTCATGGAA+TGG - chr5_1:68126682-68126701 Msa0746120:intron 35.0%
CATAGTATTTGGAAGGTAAC+AGG + chr5_1:68126811-68126830 None:intergenic 35.0%
GTAATTGTTGAAGACATAGG+AGG + chr5_1:68126844-68126863 None:intergenic 35.0%
TATCAGAAGTTCAAGCACAA+AGG - chr5_1:68126864-68126883 Msa0746120:intron 35.0%
TTTACTTGGATCAAGATCAC+TGG + chr5_1:68126922-68126941 None:intergenic 35.0%
!! GAGAAGTATTGGTTCTATAG+AGG + chr5_1:68127025-68127044 None:intergenic 35.0%
TATCCCTTGAATGAAGTTTG+GGG - chr5_1:68127353-68127372 Msa0746120:intron 35.0%
TTACCCCAAACTTCATTCAA+GGG + chr5_1:68127359-68127378 None:intergenic 35.0%
ATTACCCCAAACTTCATTCA+AGG + chr5_1:68127360-68127379 None:intergenic 35.0%
TTCACACCATAGAATGTAGT+TGG + chr5_1:68127560-68127579 None:intergenic 35.0%
CTAGCTGAATGTGATAAACT+TGG + chr5_1:68126564-68126583 None:intergenic 35.0%
ATTCAGCTAGAGAGTTTACT+TGG - chr5_1:68126574-68126593 Msa0746120:three_prime_UTR 35.0%
TTTCAGGACAATTCATGGAA+TGG - chr5_1:68126682-68126701 Msa0746120:intron 35.0%
CATAGTATTTGGAAGGTAAC+AGG + chr5_1:68126811-68126830 None:intergenic 35.0%
GTAATTGTTGAAGACATAGG+AGG + chr5_1:68126844-68126863 None:intergenic 35.0%
TATCAGAAGTTCAAGCACAA+AGG - chr5_1:68126864-68126883 Msa0746120:intron 35.0%
TTTACTTGGATCAAGATCAC+TGG + chr5_1:68126922-68126941 None:intergenic 35.0%
!! GAGAAGTATTGGTTCTATAG+AGG + chr5_1:68127025-68127044 None:intergenic 35.0%
TATCCCTTGAATGAAGTTTG+GGG - chr5_1:68127353-68127372 Msa0746120:intron 35.0%
TTACCCCAAACTTCATTCAA+GGG + chr5_1:68127359-68127378 None:intergenic 35.0%
ATTACCCCAAACTTCATTCA+AGG + chr5_1:68127360-68127379 None:intergenic 35.0%
TTCACACCATAGAATGTAGT+TGG + chr5_1:68127560-68127579 None:intergenic 35.0%
TGAGGAATTCATGGTTCTTC+AGG + chr5_1:68126454-68126473 None:intergenic 40.0%
ACCTGATGCTTGAAAAGGTA+GGG + chr5_1:68126511-68126530 None:intergenic 40.0%
TACCTGATGCTTGAAAAGGT+AGG + chr5_1:68126512-68126531 None:intergenic 40.0%
TATTTGGAAGGTAACAGGGT+TGG + chr5_1:68126806-68126825 None:intergenic 40.0%
GGTCCTTCATAGTATTTGGA+AGG + chr5_1:68126818-68126837 None:intergenic 40.0%
GCATGGTCCTTCATAGTATT+TGG + chr5_1:68126822-68126841 None:intergenic 40.0%
AGTGTGACACTAACACGTAT+TGG + chr5_1:68127068-68127087 None:intergenic 40.0%
! AAAGGCTTTTGTGTCCCATA+TGG + chr5_1:68127212-68127231 None:intergenic 40.0%
! ATGTAGTTGGCATTTAGGGT+GGG + chr5_1:68127547-68127566 None:intergenic 40.0%
AATGTAGTTGGCATTTAGGG+TGG + chr5_1:68127548-68127567 None:intergenic 40.0%
TGAGGAATTCATGGTTCTTC+AGG + chr5_1:68126454-68126473 None:intergenic 40.0%
ACCTGATGCTTGAAAAGGTA+GGG + chr5_1:68126511-68126530 None:intergenic 40.0%
TACCTGATGCTTGAAAAGGT+AGG + chr5_1:68126512-68126531 None:intergenic 40.0%
TATTTGGAAGGTAACAGGGT+TGG + chr5_1:68126806-68126825 None:intergenic 40.0%
GGTCCTTCATAGTATTTGGA+AGG + chr5_1:68126818-68126837 None:intergenic 40.0%
GCATGGTCCTTCATAGTATT+TGG + chr5_1:68126822-68126841 None:intergenic 40.0%
AGTGTGACACTAACACGTAT+TGG + chr5_1:68127068-68127087 None:intergenic 40.0%
! AAAGGCTTTTGTGTCCCATA+TGG + chr5_1:68127212-68127231 None:intergenic 40.0%
! ATGTAGTTGGCATTTAGGGT+GGG + chr5_1:68127547-68127566 None:intergenic 40.0%
AATGTAGTTGGCATTTAGGG+TGG + chr5_1:68127548-68127567 None:intergenic 40.0%
CTTGAAAAGGTAGGGTCACA+AGG + chr5_1:68126503-68126522 None:intergenic 45.0%
!! ACCCTACCTTTTCAAGCATC+AGG - chr5_1:68126507-68126526 Msa0746120:three_prime_UTR 45.0%
!! ATGGATTGGGCTTGTCATGT+TGG - chr5_1:68126750-68126769 Msa0746120:intron 45.0%
TGTTGAAGACATAGGAGGCA+TGG + chr5_1:68126839-68126858 None:intergenic 45.0%
CAGAAGTTCAAGCACAAAGG+AGG - chr5_1:68126867-68126886 Msa0746120:intron 45.0%
! ACGTCTTCGAGGAGAAGTAT+TGG + chr5_1:68127036-68127055 None:intergenic 45.0%
CTTACACAGACACCGACAAA+GGG + chr5_1:68127173-68127192 None:intergenic 45.0%
ACTTACACAGACACCGACAA+AGG + chr5_1:68127174-68127193 None:intergenic 45.0%
CTTGAAAAGGTAGGGTCACA+AGG + chr5_1:68126503-68126522 None:intergenic 45.0%
!! ACCCTACCTTTTCAAGCATC+AGG - chr5_1:68126507-68126526 Msa0746120:three_prime_UTR 45.0%
!! ATGGATTGGGCTTGTCATGT+TGG - chr5_1:68126750-68126769 Msa0746120:intron 45.0%
TGTTGAAGACATAGGAGGCA+TGG + chr5_1:68126839-68126858 None:intergenic 45.0%
CAGAAGTTCAAGCACAAAGG+AGG - chr5_1:68126867-68126886 Msa0746120:intron 45.0%
! ACGTCTTCGAGGAGAAGTAT+TGG + chr5_1:68127036-68127055 None:intergenic 45.0%
CTTACACAGACACCGACAAA+GGG + chr5_1:68127173-68127192 None:intergenic 45.0%
ACTTACACAGACACCGACAA+AGG + chr5_1:68127174-68127193 None:intergenic 45.0%
CCTCTGCAGATCATCATCTG+GGG + chr5_1:68126727-68126746 None:intergenic 50.0%
CCCTCTGCAGATCATCATCT+GGG + chr5_1:68126728-68126747 None:intergenic 50.0%
CCCAGATGATGATCTGCAGA+GGG - chr5_1:68126725-68126744 Msa0746120:intron 50.0%
TGATGATCTGCAGAGGGCTA+TGG - chr5_1:68126731-68126750 Msa0746120:intron 50.0%
ATCTGCAGAGGGCTATGGAT+TGG - chr5_1:68126736-68126755 Msa0746120:intron 50.0%
TCTGCAGAGGGCTATGGATT+GGG - chr5_1:68126737-68126756 Msa0746120:intron 50.0%
GATTGGGCTTGTCATGTTGG+TGG - chr5_1:68126753-68126772 Msa0746120:intron 50.0%
GTCAGTATCACGCCCTTTGT+CGG - chr5_1:68127158-68127177 Msa0746120:CDS 50.0%
! GTTGGCATTTAGGGTGGGAA+AGG + chr5_1:68127542-68127561 None:intergenic 50.0%
CCTCTGCAGATCATCATCTG+GGG + chr5_1:68126727-68126746 None:intergenic 50.0%
CCCTCTGCAGATCATCATCT+GGG + chr5_1:68126728-68126747 None:intergenic 50.0%
CCCAGATGATGATCTGCAGA+GGG - chr5_1:68126725-68126744 Msa0746120:intron 50.0%
TGATGATCTGCAGAGGGCTA+TGG - chr5_1:68126731-68126750 Msa0746120:intron 50.0%
ATCTGCAGAGGGCTATGGAT+TGG - chr5_1:68126736-68126755 Msa0746120:intron 50.0%
TCTGCAGAGGGCTATGGATT+GGG - chr5_1:68126737-68126756 Msa0746120:intron 50.0%
GATTGGGCTTGTCATGTTGG+TGG - chr5_1:68126753-68126772 Msa0746120:intron 50.0%
GTCAGTATCACGCCCTTTGT+CGG - chr5_1:68127158-68127177 Msa0746120:CDS 50.0%
! GTTGGCATTTAGGGTGGGAA+AGG + chr5_1:68127542-68127561 None:intergenic 50.0%
CCCCAGATGATGATCTGCAG+AGG - chr5_1:68126724-68126743 Msa0746120:intron 55.0%
GCCCTCTGCAGATCATCATC+TGG + chr5_1:68126729-68126748 None:intergenic 55.0%
GGACACTGAACACGTCTTCG+AGG + chr5_1:68127047-68127066 None:intergenic 55.0%
CCCCAGATGATGATCTGCAG+AGG - chr5_1:68126724-68126743 Msa0746120:intron 55.0%
GCCCTCTGCAGATCATCATC+TGG + chr5_1:68126729-68126748 None:intergenic 55.0%
GGACACTGAACACGTCTTCG+AGG + chr5_1:68127047-68127066 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 68126373 68127595 68126373 ID=Msa0746120;Name=Msa0746120
chr5_1 mRNA 68126373 68127595 68126373 ID=Msa0746120-mRNA-1;Parent=Msa0746120;Name=Msa0746120-mRNA-1;_AED=0.25;_eAED=0.25;_QI=89|1|1|1|1|1|3|233|116
chr5_1 exon 68126373 68126640 68126373 ID=Msa0746120-mRNA-1:exon:20309;Parent=Msa0746120-mRNA-1
chr5_1 exon 68127032 68127280 68127032 ID=Msa0746120-mRNA-1:exon:20308;Parent=Msa0746120-mRNA-1
chr5_1 exon 68127440 68127595 68127440 ID=Msa0746120-mRNA-1:exon:20307;Parent=Msa0746120-mRNA-1
chr5_1 five_prime_UTR 68127507 68127595 68127507 ID=Msa0746120-mRNA-1:five_prime_utr;Parent=Msa0746120-mRNA-1
chr5_1 CDS 68127440 68127506 68127440 ID=Msa0746120-mRNA-1:cds;Parent=Msa0746120-mRNA-1
chr5_1 CDS 68127032 68127280 68127032 ID=Msa0746120-mRNA-1:cds;Parent=Msa0746120-mRNA-1
chr5_1 CDS 68126606 68126640 68126606 ID=Msa0746120-mRNA-1:cds;Parent=Msa0746120-mRNA-1
chr5_1 three_prime_UTR 68126373 68126605 68126373 ID=Msa0746120-mRNA-1:three_prime_utr;Parent=Msa0746120-mRNA-1
Gene Sequence

>Msa0746120

ATGAATTCCTCAATGATCAAAATTTTGATAGCTATGCTCCTTGTGACCCTACCTTTTCAAGCATCAGGACAATTCATGGAATGGTGCATAGCTGATGAGCAGACCCCAGATGATGATCTGCAGAGGGCTATGGATTGGGCTTGTCATGTTGGTGGAGCAAATTGTAGCAACATACAAGTGAACCAACCCTGTTACCTTCCAAATACTATGAAGGACCATGCCTCCTATGTCTTCAACAATTACTATCAGAAGTTCAAGCACAAAGGAGGATCTTGCTATTTTAACTCTGCTGCAATAACCAGTGATCTTGATCCAAGTCATGGCTCTTGCAAGTTTGAGTATATCCCTTGA
Protein sequence

>Msa0746120

MNSSMIKILIAMLLVTLPFQASGQFMEWCIADEQTPDDDLQRAMDWACHVGGANCSNIQVNQPCYLPNTMKDHASYVFNNYYQKFKHKGGSCYFNSAAITSDLDPSHGSCKFEYIP*