AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0094480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0094480 MtrunA17_Chr5g0438181 100.000 69 0 0 1 69 1 69 1.09e-44 139
Msa0094480 MtrunA17_Chr4g0071611 100.000 69 0 0 1 69 1 69 1.72e-44 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0094480 AT4G24920.1 92.647 68 5 0 1 68 1 68 4.96e-41 128
Msa0094480 AT5G50460.1 92.647 68 5 0 1 68 1 68 4.96e-41 128
Msa0094480 AT3G48570.1 86.957 69 9 0 1 69 1 69 2.17e-39 124

Find 41 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAACGATGATGTTGTTAAT+TGG 0.213895 1_2:-77694497 None:intergenic
ATTCTTAGATCGAAAGAAAA+TGG 0.228279 1_2:+77694203 Msa0094480:intron
TGATTGGTATCTATGTAAAT+TGG 0.291365 1_2:+77696188 Msa0094480:three_prime_UTR
TGGTTATTTCCACAGGATAA+TGG 0.303863 1_2:+77695906 Msa0094480:intron
CCTAACGATCGAAGTCTTAG+TGG 0.358218 1_2:-77694017 None:intergenic
CGCTTTGCTCCTTGAACAAT+GGG 0.363239 1_2:-77696118 None:intergenic
TCGTATTGTAGAATTCTCTA+AGG 0.363993 1_2:+77694403 Msa0094480:intron
AGACCTTTGTTCTGTGTGTT+GGG 0.368248 1_2:-77693987 None:intergenic
AGGAGCAAAGCGTTTAGATA+TGG 0.391783 1_2:+77696129 Msa0094480:three_prime_UTR
TGCCATCGGTTTCGTTGTGA+TGG 0.408671 1_2:+77694439 Msa0094480:CDS
TACCTTTGCGATCGGGTTTG+TGG 0.417825 1_2:-77694296 None:intergenic
CCACTAAGACTTCGATCGTT+AGG 0.420908 1_2:+77694017 Msa0094480:five_prime_UTR
AACAACATCATCGTTGGATC+TGG 0.426526 1_2:+77694503 Msa0094480:CDS
CATCATCGTTGGATCTGGTT+AGG 0.451612 1_2:+77694508 Msa0094480:CDS
GAGACCTTTGTTCTGTGTGT+TGG 0.472045 1_2:-77693988 None:intergenic
ACGCTTTGCTCCTTGAACAA+TGG 0.473873 1_2:-77696119 None:intergenic
TTCGTTGTGATGGGATTCGT+TGG 0.482699 1_2:+77694449 Msa0094480:CDS
GCCATCGGTTTCGTTGTGAT+GGG 0.492846 1_2:+77694440 Msa0094480:CDS
CCAATTAACAACATCATCGT+TGG 0.497266 1_2:+77694497 Msa0094480:CDS
GTTGCTGTGCGCACTGCCAT+CGG 0.516428 1_2:+77694425 Msa0094480:CDS
GACCTTTGTTCTGTGTGTTG+GGG 0.517962 1_2:-77693986 None:intergenic
TGGATCGAAAACAGAATCAA+TGG 0.519751 1_2:-77694229 None:intergenic
ATGGAAACTACAATCGCCTC+AGG 0.536961 1_2:+77695925 Msa0094480:three_prime_UTR
CACCTTCATACTTTATGTGA+GGG 0.540861 1_2:-77696215 None:intergenic
TCTGCAACAAAACGAAAGGT+TGG 0.543759 1_2:-77693954 None:intergenic
TGAAGCGTATGGAGGCAGTA+GGG 0.546648 1_2:+77695959 Msa0094480:three_prime_UTR
GTGAAGCGTATGGAGGCAGT+AGG 0.563634 1_2:+77695958 Msa0094480:three_prime_UTR
TTTGTCTGCAACAAAACGAA+AGG 0.571069 1_2:-77693958 None:intergenic
CACTGAATGTTCTATTAAAG+CGG 0.574715 1_2:-77695986 None:intergenic
TCACCTTCATACTTTATGTG+AGG 0.586315 1_2:-77696216 None:intergenic
AATTCAAGTTGTGAAGCGTA+TGG 0.586553 1_2:+77695948 Msa0094480:three_prime_UTR
ACTGAATGTTCTATTAAAGC+GGG 0.590322 1_2:-77695985 None:intergenic
AATTCGCTAAGGACAGCGTA+AGG 0.605053 1_2:+77694259 Msa0094480:CDS
CTTCACAACTTGAATTCCTG+AGG 0.631270 1_2:-77695941 None:intergenic
GAAGCGTATGGAGGCAGTAG+GGG 0.637258 1_2:+77695960 Msa0094480:three_prime_UTR
TGCCACAAACCCGATCGCAA+AGG 0.641795 1_2:+77694294 Msa0094480:CDS
TCCCATCACAACGAAACCGA+TGG 0.648968 1_2:-77694441 None:intergenic
TCCACTCAGAGAATTCGCTA+AGG 0.652105 1_2:+77694248 Msa0094480:CDS
ATCCCCAACACACAGAACAA+AGG 0.654430 1_2:+77693984 Msa0094480:five_prime_UTR
TCAAGTTGTGAAGCGTATGG+AGG 0.660190 1_2:+77695951 Msa0094480:three_prime_UTR
TCCTTAGCGAATTCTCTGAG+TGG 0.675399 1_2:-77694249 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAGAAAAAAAAGAAATA+TGG - chr1_2:77696047-77696066 None:intergenic 10.0%
!!! TGTAAAATTTTATTTTTACA+TGG + chr1_2:77696082-77696101 Msa0094480:three_prime_UTR 10.0%
!!! GTAAAATTTTATTTTTACAT+GGG + chr1_2:77696083-77696102 Msa0094480:three_prime_UTR 10.0%
!!! TTTTAGAAAAAAAAGAAATA+TGG - chr1_2:77696047-77696066 None:intergenic 10.0%
!!! TGTAAAATTTTATTTTTACA+TGG + chr1_2:77696082-77696101 Msa0094480:three_prime_UTR 10.0%
!!! GTAAAATTTTATTTTTACAT+GGG + chr1_2:77696083-77696102 Msa0094480:three_prime_UTR 10.0%
!! ATTCAAAGTAAACAAAGAAA+GGG - chr1_2:77694058-77694077 None:intergenic 20.0%
!! TATTCAAAGTAAACAAAGAA+AGG - chr1_2:77694059-77694078 None:intergenic 20.0%
!! ATCTAAGAATTGAAACAAAT+TGG - chr1_2:77694193-77694212 None:intergenic 20.0%
!! AAATATGTCCTTCAAATTAA+AGG - chr1_2:77694589-77694608 None:intergenic 20.0%
!! AATAATTGTTTATCCAAACA+GGG - chr1_2:77694670-77694689 None:intergenic 20.0%
!! AAATAATTGTTTATCCAAAC+AGG - chr1_2:77694671-77694690 None:intergenic 20.0%
!! GACTATTTGATAAAACTATA+TGG - chr1_2:77694871-77694890 None:intergenic 20.0%
!!! AGCTGTTTTAATATAAATCA+AGG + chr1_2:77694923-77694942 Msa0094480:intron 20.0%
!!! GCTGTTTTAATATAAATCAA+GGG + chr1_2:77694924-77694943 Msa0094480:intron 20.0%
!!! AAGTATGTTTGTTGTATTTA+TGG + chr1_2:77694954-77694973 Msa0094480:intron 20.0%
!! ATGATTAATTGATTACATCT+TGG + chr1_2:77695197-77695216 Msa0094480:intron 20.0%
!! GAAAATAATTATACAATCTC+TGG - chr1_2:77695512-77695531 None:intergenic 20.0%
!!! ATAATTATTTTCTTAGTGAC+CGG + chr1_2:77695520-77695539 Msa0094480:intron 20.0%
!! ATTCAAAGTAAACAAAGAAA+GGG - chr1_2:77694058-77694077 None:intergenic 20.0%
!! TATTCAAAGTAAACAAAGAA+AGG - chr1_2:77694059-77694078 None:intergenic 20.0%
!! ATCTAAGAATTGAAACAAAT+TGG - chr1_2:77694193-77694212 None:intergenic 20.0%
!! AAATATGTCCTTCAAATTAA+AGG - chr1_2:77694589-77694608 None:intergenic 20.0%
!! AATAATTGTTTATCCAAACA+GGG - chr1_2:77694670-77694689 None:intergenic 20.0%
!! AAATAATTGTTTATCCAAAC+AGG - chr1_2:77694671-77694690 None:intergenic 20.0%
!! GACTATTTGATAAAACTATA+TGG - chr1_2:77694871-77694890 None:intergenic 20.0%
!!! AGCTGTTTTAATATAAATCA+AGG + chr1_2:77694923-77694942 Msa0094480:intron 20.0%
!!! GCTGTTTTAATATAAATCAA+GGG + chr1_2:77694924-77694943 Msa0094480:intron 20.0%
!!! AAGTATGTTTGTTGTATTTA+TGG + chr1_2:77694954-77694973 Msa0094480:intron 20.0%
!! ATGATTAATTGATTACATCT+TGG + chr1_2:77695197-77695216 Msa0094480:intron 20.0%
!! GAAAATAATTATACAATCTC+TGG - chr1_2:77695512-77695531 None:intergenic 20.0%
!!! ATAATTATTTTCTTAGTGAC+CGG + chr1_2:77695520-77695539 Msa0094480:intron 20.0%
! ATTCTTAGATCGAAAGAAAA+TGG + chr1_2:77694203-77694222 Msa0094480:intron 25.0%
!!! TTATCCTTCCTTTAATTTGA+AGG + chr1_2:77694578-77694597 Msa0094480:intron 25.0%
! AATTTGAAGGACATATTTGA+TGG + chr1_2:77694591-77694610 Msa0094480:intron 25.0%
! AACAGAAGTAAACTTAAAAC+AGG - chr1_2:77695348-77695367 None:intergenic 25.0%
! ACATATGATACGATTTCTAT+TGG - chr1_2:77695824-77695843 None:intergenic 25.0%
! AATCGTATCATATGTTACTA+AGG + chr1_2:77695829-77695848 Msa0094480:intron 25.0%
! TGATTGGTATCTATGTAAAT+TGG + chr1_2:77696188-77696207 Msa0094480:three_prime_UTR 25.0%
! ATTCTTAGATCGAAAGAAAA+TGG + chr1_2:77694203-77694222 Msa0094480:intron 25.0%
!!! TTATCCTTCCTTTAATTTGA+AGG + chr1_2:77694578-77694597 Msa0094480:intron 25.0%
! AATTTGAAGGACATATTTGA+TGG + chr1_2:77694591-77694610 Msa0094480:intron 25.0%
! AACAGAAGTAAACTTAAAAC+AGG - chr1_2:77695348-77695367 None:intergenic 25.0%
! ACATATGATACGATTTCTAT+TGG - chr1_2:77695824-77695843 None:intergenic 25.0%
! AATCGTATCATATGTTACTA+AGG + chr1_2:77695829-77695848 Msa0094480:intron 25.0%
! TGATTGGTATCTATGTAAAT+TGG + chr1_2:77696188-77696207 Msa0094480:three_prime_UTR 25.0%
! GAAAGACAATGACTTTTGAT+TGG + chr1_2:77693888-77693907 Msa0094480:five_prime_UTR 30.0%
ATCTAGTAGATCGAAATCAA+AGG - chr1_2:77694100-77694119 None:intergenic 30.0%
!!! AGTTTTTTTACCTTTGCGAT+CGG - chr1_2:77694307-77694326 None:intergenic 30.0%
AAGATCAAAGAGGAATGAAA+GGG - chr1_2:77694336-77694355 None:intergenic 30.0%
TCATAAAACCAAGATCAAAG+AGG - chr1_2:77694346-77694365 None:intergenic 30.0%
! TTTTATGAATCTCTGTGCTA+TGG + chr1_2:77694358-77694377 Msa0094480:intron 30.0%
TCGTATTGTAGAATTCTCTA+AGG + chr1_2:77694403-77694422 Msa0094480:intron 30.0%
AAAATTAGAGTTCAGTGTGA+GGG - chr1_2:77694546-77694565 None:intergenic 30.0%
AAAAATTAGAGTTCAGTGTG+AGG - chr1_2:77694547-77694566 None:intergenic 30.0%
ATGTCCTTCAAATTAAAGGA+AGG - chr1_2:77694585-77694604 None:intergenic 30.0%
GACATATTTGATGGAATGAT+TGG + chr1_2:77694600-77694619 Msa0094480:intron 30.0%
! TTGAAACGATTTGAGATCTT+AGG + chr1_2:77694644-77694663 Msa0094480:intron 30.0%
TAGCTTATGAAAACAGCTTA+TGG - chr1_2:77694789-77694808 None:intergenic 30.0%
ACAAGTAAATCCATTATGCA+GGG - chr1_2:77694984-77695003 None:intergenic 30.0%
!! TTGCTTTTGCTTGTTCTAAA+TGG + chr1_2:77695129-77695148 Msa0094480:intron 30.0%
TGTTGAATGATAACACCTTA+TGG + chr1_2:77695231-77695250 Msa0094480:intron 30.0%
! AGCAACTTTTTGTAACCATA+AGG - chr1_2:77695249-77695268 None:intergenic 30.0%
TGAACATATTTGCGACAATA+TGG + chr1_2:77695385-77695404 Msa0094480:intron 30.0%
CATTACATTACAGAAATAGC+TGG - chr1_2:77695577-77695596 None:intergenic 30.0%
TGTAATGTAATGAGAGAGTT+TGG + chr1_2:77695585-77695604 Msa0094480:intron 30.0%
! ACTAATGTCAAGGATGTTTT+TGG + chr1_2:77695629-77695648 Msa0094480:intron 30.0%
ACTGAATGTTCTATTAAAGC+GGG - chr1_2:77695988-77696007 None:intergenic 30.0%
CACTGAATGTTCTATTAAAG+CGG - chr1_2:77695989-77696008 None:intergenic 30.0%
! AAACTCACAAAAAGCATGAT+TGG + chr1_2:77696172-77696191 Msa0094480:three_prime_UTR 30.0%
! GAAAGACAATGACTTTTGAT+TGG + chr1_2:77693888-77693907 Msa0094480:five_prime_UTR 30.0%
ATCTAGTAGATCGAAATCAA+AGG - chr1_2:77694100-77694119 None:intergenic 30.0%
!!! AGTTTTTTTACCTTTGCGAT+CGG - chr1_2:77694307-77694326 None:intergenic 30.0%
AAGATCAAAGAGGAATGAAA+GGG - chr1_2:77694336-77694355 None:intergenic 30.0%
TCATAAAACCAAGATCAAAG+AGG - chr1_2:77694346-77694365 None:intergenic 30.0%
! TTTTATGAATCTCTGTGCTA+TGG + chr1_2:77694358-77694377 Msa0094480:intron 30.0%
TCGTATTGTAGAATTCTCTA+AGG + chr1_2:77694403-77694422 Msa0094480:intron 30.0%
AAAATTAGAGTTCAGTGTGA+GGG - chr1_2:77694546-77694565 None:intergenic 30.0%
AAAAATTAGAGTTCAGTGTG+AGG - chr1_2:77694547-77694566 None:intergenic 30.0%
ATGTCCTTCAAATTAAAGGA+AGG - chr1_2:77694585-77694604 None:intergenic 30.0%
GACATATTTGATGGAATGAT+TGG + chr1_2:77694600-77694619 Msa0094480:intron 30.0%
! TTGAAACGATTTGAGATCTT+AGG + chr1_2:77694644-77694663 Msa0094480:intron 30.0%
TAGCTTATGAAAACAGCTTA+TGG - chr1_2:77694789-77694808 None:intergenic 30.0%
ACAAGTAAATCCATTATGCA+GGG - chr1_2:77694984-77695003 None:intergenic 30.0%
!! TTGCTTTTGCTTGTTCTAAA+TGG + chr1_2:77695129-77695148 Msa0094480:intron 30.0%
TGTTGAATGATAACACCTTA+TGG + chr1_2:77695231-77695250 Msa0094480:intron 30.0%
! AGCAACTTTTTGTAACCATA+AGG - chr1_2:77695249-77695268 None:intergenic 30.0%
TGAACATATTTGCGACAATA+TGG + chr1_2:77695385-77695404 Msa0094480:intron 30.0%
CATTACATTACAGAAATAGC+TGG - chr1_2:77695577-77695596 None:intergenic 30.0%
TGTAATGTAATGAGAGAGTT+TGG + chr1_2:77695585-77695604 Msa0094480:intron 30.0%
! ACTAATGTCAAGGATGTTTT+TGG + chr1_2:77695629-77695648 Msa0094480:intron 30.0%
ACTGAATGTTCTATTAAAGC+GGG - chr1_2:77695988-77696007 None:intergenic 30.0%
CACTGAATGTTCTATTAAAG+CGG - chr1_2:77695989-77696008 None:intergenic 30.0%
! AAACTCACAAAAAGCATGAT+TGG + chr1_2:77696172-77696191 Msa0094480:three_prime_UTR 30.0%
TTTGTCTGCAACAAAACGAA+AGG - chr1_2:77693961-77693980 None:intergenic 35.0%
TGGATCGAAAACAGAATCAA+TGG - chr1_2:77694232-77694251 None:intergenic 35.0%
!!! GTTTTTTTACCTTTGCGATC+GGG - chr1_2:77694306-77694325 None:intergenic 35.0%
CAAGATCAAAGAGGAATGAA+AGG - chr1_2:77694337-77694356 None:intergenic 35.0%
CTTTCATTCCTCTTTGATCT+TGG + chr1_2:77694335-77694354 Msa0094480:intron 35.0%
CCAACGATGATGTTGTTAAT+TGG - chr1_2:77694500-77694519 None:intergenic 35.0%
CCAATTAACAACATCATCGT+TGG + chr1_2:77694497-77694516 Msa0094480:CDS 35.0%
! TTATGGTTTTCCCTGCATAA+TGG + chr1_2:77694971-77694990 Msa0094480:intron 35.0%
CACAAGTAAATCCATTATGC+AGG - chr1_2:77694985-77695004 None:intergenic 35.0%
AGCTTTCTCTTTGTCATGAT+TGG + chr1_2:77695069-77695088 Msa0094480:intron 35.0%
GTTAGTGACTTTCTCTTTGT+TGG + chr1_2:77695091-77695110 Msa0094480:intron 35.0%
!!! GACTTTCTCTTTGTTGGTTT+TGG + chr1_2:77695097-77695116 Msa0094480:intron 35.0%
ATGACGATGTAATTAACGCT+TGG + chr1_2:77695886-77695905 Msa0094480:intron 35.0%
! TGGTTATTTCCACAGGATAA+TGG + chr1_2:77695906-77695925 Msa0094480:intron 35.0%
TTGTAGTTTCCATTATCCTG+TGG - chr1_2:77695918-77695937 None:intergenic 35.0%
!! AATTCAAGTTGTGAAGCGTA+TGG + chr1_2:77695948-77695967 Msa0094480:three_prime_UTR 35.0%
TTTGTCTGCAACAAAACGAA+AGG - chr1_2:77693961-77693980 None:intergenic 35.0%
TGGATCGAAAACAGAATCAA+TGG - chr1_2:77694232-77694251 None:intergenic 35.0%
!!! GTTTTTTTACCTTTGCGATC+GGG - chr1_2:77694306-77694325 None:intergenic 35.0%
CAAGATCAAAGAGGAATGAA+AGG - chr1_2:77694337-77694356 None:intergenic 35.0%
CTTTCATTCCTCTTTGATCT+TGG + chr1_2:77694335-77694354 Msa0094480:intron 35.0%
CCAACGATGATGTTGTTAAT+TGG - chr1_2:77694500-77694519 None:intergenic 35.0%
CCAATTAACAACATCATCGT+TGG + chr1_2:77694497-77694516 Msa0094480:CDS 35.0%
! TTATGGTTTTCCCTGCATAA+TGG + chr1_2:77694971-77694990 Msa0094480:intron 35.0%
CACAAGTAAATCCATTATGC+AGG - chr1_2:77694985-77695004 None:intergenic 35.0%
AGCTTTCTCTTTGTCATGAT+TGG + chr1_2:77695069-77695088 Msa0094480:intron 35.0%
GTTAGTGACTTTCTCTTTGT+TGG + chr1_2:77695091-77695110 Msa0094480:intron 35.0%
!!! GACTTTCTCTTTGTTGGTTT+TGG + chr1_2:77695097-77695116 Msa0094480:intron 35.0%
ATGACGATGTAATTAACGCT+TGG + chr1_2:77695886-77695905 Msa0094480:intron 35.0%
! TGGTTATTTCCACAGGATAA+TGG + chr1_2:77695906-77695925 Msa0094480:intron 35.0%
TTGTAGTTTCCATTATCCTG+TGG - chr1_2:77695918-77695937 None:intergenic 35.0%
!! AATTCAAGTTGTGAAGCGTA+TGG + chr1_2:77695948-77695967 Msa0094480:three_prime_UTR 35.0%
TCTGCAACAAAACGAAAGGT+TGG - chr1_2:77693957-77693976 None:intergenic 40.0%
AGACCTTTGTTCTGTGTGTT+GGG - chr1_2:77693990-77694009 None:intergenic 40.0%
AACAACATCATCGTTGGATC+TGG + chr1_2:77694503-77694522 Msa0094480:CDS 40.0%
GTCATTACACAAGCTCCTTA+GGG - chr1_2:77695555-77695574 None:intergenic 40.0%
GAGAGTTTGGTATTAGTCGT+AGG + chr1_2:77695598-77695617 Msa0094480:intron 40.0%
! TAACGCTTGGTTATTTCCAC+AGG + chr1_2:77695899-77695918 Msa0094480:intron 40.0%
CTTCACAACTTGAATTCCTG+AGG - chr1_2:77695944-77695963 None:intergenic 40.0%
! GACTTTTGACCCATTGTTCA+AGG + chr1_2:77696109-77696128 Msa0094480:three_prime_UTR 40.0%
! AGGAGCAAAGCGTTTAGATA+TGG + chr1_2:77696129-77696148 Msa0094480:three_prime_UTR 40.0%
TCTGCAACAAAACGAAAGGT+TGG - chr1_2:77693957-77693976 None:intergenic 40.0%
AGACCTTTGTTCTGTGTGTT+GGG - chr1_2:77693990-77694009 None:intergenic 40.0%
AACAACATCATCGTTGGATC+TGG + chr1_2:77694503-77694522 Msa0094480:CDS 40.0%
GTCATTACACAAGCTCCTTA+GGG - chr1_2:77695555-77695574 None:intergenic 40.0%
GAGAGTTTGGTATTAGTCGT+AGG + chr1_2:77695598-77695617 Msa0094480:intron 40.0%
! TAACGCTTGGTTATTTCCAC+AGG + chr1_2:77695899-77695918 Msa0094480:intron 40.0%
CTTCACAACTTGAATTCCTG+AGG - chr1_2:77695944-77695963 None:intergenic 40.0%
! GACTTTTGACCCATTGTTCA+AGG + chr1_2:77696109-77696128 Msa0094480:three_prime_UTR 40.0%
! AGGAGCAAAGCGTTTAGATA+TGG + chr1_2:77696129-77696148 Msa0094480:three_prime_UTR 40.0%
ATCCCCAACACACAGAACAA+AGG + chr1_2:77693984-77694003 Msa0094480:five_prime_UTR 45.0%
GACCTTTGTTCTGTGTGTTG+GGG - chr1_2:77693989-77694008 None:intergenic 45.0%
GAGACCTTTGTTCTGTGTGT+TGG - chr1_2:77693991-77694010 None:intergenic 45.0%
CCTAACGATCGAAGTCTTAG+TGG - chr1_2:77694020-77694039 None:intergenic 45.0%
CCACTAAGACTTCGATCGTT+AGG + chr1_2:77694017-77694036 Msa0094480:five_prime_UTR 45.0%
TCCACTCAGAGAATTCGCTA+AGG + chr1_2:77694248-77694267 Msa0094480:CDS 45.0%
TCCTTAGCGAATTCTCTGAG+TGG - chr1_2:77694252-77694271 None:intergenic 45.0%
AATTCGCTAAGGACAGCGTA+AGG + chr1_2:77694259-77694278 Msa0094480:CDS 45.0%
! TTCGTTGTGATGGGATTCGT+TGG + chr1_2:77694449-77694468 Msa0094480:CDS 45.0%
CATCATCGTTGGATCTGGTT+AGG + chr1_2:77694508-77694527 Msa0094480:CDS 45.0%
TTGAGATCTTAGGCCCTGTT+TGG + chr1_2:77694654-77694673 Msa0094480:intron 45.0%
CGACTTGCATAAGCACTTGT+GGG - chr1_2:77694893-77694912 None:intergenic 45.0%
! TCGACTTGCATAAGCACTTG+TGG - chr1_2:77694894-77694913 None:intergenic 45.0%
GGTCATTACACAAGCTCCTT+AGG - chr1_2:77695556-77695575 None:intergenic 45.0%
TACACACTTTGCTCGAGACA+CGG - chr1_2:77695865-77695884 None:intergenic 45.0%
ATGGAAACTACAATCGCCTC+AGG + chr1_2:77695925-77695944 Msa0094480:three_prime_UTR 45.0%
! TCAAGTTGTGAAGCGTATGG+AGG + chr1_2:77695951-77695970 Msa0094480:three_prime_UTR 45.0%
CGCTTTGCTCCTTGAACAAT+GGG - chr1_2:77696121-77696140 None:intergenic 45.0%
ACGCTTTGCTCCTTGAACAA+TGG - chr1_2:77696122-77696141 None:intergenic 45.0%
ATCCCCAACACACAGAACAA+AGG + chr1_2:77693984-77694003 Msa0094480:five_prime_UTR 45.0%
GACCTTTGTTCTGTGTGTTG+GGG - chr1_2:77693989-77694008 None:intergenic 45.0%
GAGACCTTTGTTCTGTGTGT+TGG - chr1_2:77693991-77694010 None:intergenic 45.0%
CCTAACGATCGAAGTCTTAG+TGG - chr1_2:77694020-77694039 None:intergenic 45.0%
CCACTAAGACTTCGATCGTT+AGG + chr1_2:77694017-77694036 Msa0094480:five_prime_UTR 45.0%
TCCACTCAGAGAATTCGCTA+AGG + chr1_2:77694248-77694267 Msa0094480:CDS 45.0%
TCCTTAGCGAATTCTCTGAG+TGG - chr1_2:77694252-77694271 None:intergenic 45.0%
AATTCGCTAAGGACAGCGTA+AGG + chr1_2:77694259-77694278 Msa0094480:CDS 45.0%
! TTCGTTGTGATGGGATTCGT+TGG + chr1_2:77694449-77694468 Msa0094480:CDS 45.0%
CATCATCGTTGGATCTGGTT+AGG + chr1_2:77694508-77694527 Msa0094480:CDS 45.0%
TTGAGATCTTAGGCCCTGTT+TGG + chr1_2:77694654-77694673 Msa0094480:intron 45.0%
CGACTTGCATAAGCACTTGT+GGG - chr1_2:77694893-77694912 None:intergenic 45.0%
! TCGACTTGCATAAGCACTTG+TGG - chr1_2:77694894-77694913 None:intergenic 45.0%
GGTCATTACACAAGCTCCTT+AGG - chr1_2:77695556-77695575 None:intergenic 45.0%
TACACACTTTGCTCGAGACA+CGG - chr1_2:77695865-77695884 None:intergenic 45.0%
ATGGAAACTACAATCGCCTC+AGG + chr1_2:77695925-77695944 Msa0094480:three_prime_UTR 45.0%
! TCAAGTTGTGAAGCGTATGG+AGG + chr1_2:77695951-77695970 Msa0094480:three_prime_UTR 45.0%
CGCTTTGCTCCTTGAACAAT+GGG - chr1_2:77696121-77696140 None:intergenic 45.0%
ACGCTTTGCTCCTTGAACAA+TGG - chr1_2:77696122-77696141 None:intergenic 45.0%
TACCTTTGCGATCGGGTTTG+TGG - chr1_2:77694299-77694318 None:intergenic 50.0%
!! TGCCATCGGTTTCGTTGTGA+TGG + chr1_2:77694439-77694458 Msa0094480:CDS 50.0%
!! GCCATCGGTTTCGTTGTGAT+GGG + chr1_2:77694440-77694459 Msa0094480:CDS 50.0%
TCCCATCACAACGAAACCGA+TGG - chr1_2:77694444-77694463 None:intergenic 50.0%
ACACAAGCTCCTTAGGGCAT+AGG - chr1_2:77695549-77695568 None:intergenic 50.0%
GGCTGCAGCAACTAATGTCA+AGG + chr1_2:77695619-77695638 Msa0094480:intron 50.0%
TGAAGCGTATGGAGGCAGTA+GGG + chr1_2:77695959-77695978 Msa0094480:three_prime_UTR 50.0%
TACCTTTGCGATCGGGTTTG+TGG - chr1_2:77694299-77694318 None:intergenic 50.0%
!! TGCCATCGGTTTCGTTGTGA+TGG + chr1_2:77694439-77694458 Msa0094480:CDS 50.0%
!! GCCATCGGTTTCGTTGTGAT+GGG + chr1_2:77694440-77694459 Msa0094480:CDS 50.0%
TCCCATCACAACGAAACCGA+TGG - chr1_2:77694444-77694463 None:intergenic 50.0%
ACACAAGCTCCTTAGGGCAT+AGG - chr1_2:77695549-77695568 None:intergenic 50.0%
GGCTGCAGCAACTAATGTCA+AGG + chr1_2:77695619-77695638 Msa0094480:intron 50.0%
TGAAGCGTATGGAGGCAGTA+GGG + chr1_2:77695959-77695978 Msa0094480:three_prime_UTR 50.0%
TGCCACAAACCCGATCGCAA+AGG + chr1_2:77694294-77694313 Msa0094480:CDS 55.0%
CTCCTTAGGGCATAGGTAGC+CGG - chr1_2:77695542-77695561 None:intergenic 55.0%
GTGAAGCGTATGGAGGCAGT+AGG + chr1_2:77695958-77695977 Msa0094480:three_prime_UTR 55.0%
GAAGCGTATGGAGGCAGTAG+GGG + chr1_2:77695960-77695979 Msa0094480:three_prime_UTR 55.0%
TGCCACAAACCCGATCGCAA+AGG + chr1_2:77694294-77694313 Msa0094480:CDS 55.0%
CTCCTTAGGGCATAGGTAGC+CGG - chr1_2:77695542-77695561 None:intergenic 55.0%
GTGAAGCGTATGGAGGCAGT+AGG + chr1_2:77695958-77695977 Msa0094480:three_prime_UTR 55.0%
GAAGCGTATGGAGGCAGTAG+GGG + chr1_2:77695960-77695979 Msa0094480:three_prime_UTR 55.0%
GTTGCTGTGCGCACTGCCAT+CGG + chr1_2:77694425-77694444 Msa0094480:CDS 60.0%
GACCGGCTACCTATGCCCTA+AGG + chr1_2:77695537-77695556 Msa0094480:intron 60.0%
GTTGCTGTGCGCACTGCCAT+CGG + chr1_2:77694425-77694444 Msa0094480:CDS 60.0%
GACCGGCTACCTATGCCCTA+AGG + chr1_2:77695537-77695556 Msa0094480:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 77693879 77696226 77693879 ID=Msa0094480;Name=Msa0094480
chr1_2 mRNA 77693879 77696226 77693879 ID=Msa0094480-mRNA-1;Parent=Msa0094480;Name=Msa0094480-mRNA-1;_AED=0.25;_eAED=0.25;_QI=171|1|1|1|0.33|0.25|4|306|69
chr1_2 exon 77693879 77694038 77693879 ID=Msa0094480-mRNA-1:exon:27615;Parent=Msa0094480-mRNA-1
chr1_2 exon 77694211 77694315 77694211 ID=Msa0094480-mRNA-1:exon:27616;Parent=Msa0094480-mRNA-1
chr1_2 exon 77694414 77694529 77694414 ID=Msa0094480-mRNA-1:exon:27617;Parent=Msa0094480-mRNA-1
chr1_2 exon 77695921 77696226 77695921 ID=Msa0094480-mRNA-1:exon:27618;Parent=Msa0094480-mRNA-1
chr1_2 five_prime_UTR 77693879 77694038 77693879 ID=Msa0094480-mRNA-1:five_prime_utr;Parent=Msa0094480-mRNA-1
chr1_2 five_prime_UTR 77694211 77694221 77694211 ID=Msa0094480-mRNA-1:five_prime_utr;Parent=Msa0094480-mRNA-1
chr1_2 CDS 77694222 77694315 77694222 ID=Msa0094480-mRNA-1:cds;Parent=Msa0094480-mRNA-1
chr1_2 CDS 77694414 77694529 77694414 ID=Msa0094480-mRNA-1:cds;Parent=Msa0094480-mRNA-1
chr1_2 three_prime_UTR 77695921 77696226 77695921 ID=Msa0094480-mRNA-1:three_prime_utr;Parent=Msa0094480-mRNA-1
Gene Sequence

>Msa0094480

ATGGACGCCATTGATTCTGTTTTCGATCCACTCAGAGAATTCGCTAAGGACAGCGTAAGGCTCGTCAAGCGATGCCACAAACCCGATCGCAAAGAATTCTCTAAGGTTGCTGTGCGCACTGCCATCGGTTTCGTTGTGATGGGATTCGTTGGCTTTTTCGTTAAGCTCATTTTCATTCCAATTAACAACATCATCGTTGGATCTGGTTAG
Protein sequence

>Msa0094480

MDAIDSVFDPLREFAKDSVRLVKRCHKPDRKEFSKVAVRTAIGFVVMGFVGFFVKLIFIPINNIIVGSG*