AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0100960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0100960 MtrunA17_Chr1g0149301 97.464 276 5 1 1 276 1 274 0.0 539
Msa0100960 MtrunA17_Chr3g0137851 61.686 261 73 4 1 259 1 236 1.90e-99 291
Msa0100960 MtrunA17_Chr4g0063731 58.845 277 107 3 1 275 1 272 1.57e-97 287
Msa0100960 MtrunA17_Chr4g0012631 45.196 281 101 7 1 268 1 241 3.52e-64 202
Msa0100960 MtrunA17_Chr8g0372781 38.163 283 151 4 1 270 1 272 4.22e-53 175
Msa0100960 MtrunA17_Chr7g0231141 43.056 72 40 1 135 206 5 75 4.24e-16 72.4
Msa0100960 MtrunA17_Chr7g0231151 42.708 96 31 2 1 96 1 72 1.01e-14 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0100960 AT2G26660.1 56.989 279 111 3 1 276 1 273 1.27e-94 280
Msa0100960 AT5G20150.1 56.877 269 84 4 1 269 1 237 8.73e-92 272
Msa0100960 AT5G15330.1 38.411 302 147 7 1 276 1 289 4.06e-57 186
Msa0100960 AT5G15330.2 38.411 302 147 7 1 276 70 358 2.08e-56 186
Msa0100960 AT2G45130.1 40.072 277 106 6 1 269 1 225 1.18e-55 180

Find 96 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACCGAAATTTAAATTATT+TGG 0.116635 1_3:-7398107 Msa0100960:three_prime_UTR
TGAAAATGTTCTCCATATTT+TGG 0.132016 1_3:+7398757 None:intergenic
TGGAAATTTGGCCTCCATTT+TGG 0.157714 1_3:-7398456 Msa0100960:three_prime_UTR
AATAATGATAATGAGTAATT+TGG 0.187432 1_3:+7401791 None:intergenic
ATTATTTGGTTGCAATAAAA+AGG 0.188466 1_3:-7398093 Msa0100960:three_prime_UTR
AATGAATTCGGATGGAAATT+TGG 0.203194 1_3:-7398468 Msa0100960:three_prime_UTR
GTTGAAATTGAGAAATTTAA+TGG 0.239620 1_3:-7401429 Msa0100960:CDS
CAACCAAATAATTTAAATTT+CGG 0.246816 1_3:+7398104 None:intergenic
TTCAAGACATCTTGGATAAA+AGG 0.255878 1_3:+7398981 None:intergenic
TGGATCTCGGAAAGTTCTTT+TGG 0.256770 1_3:+7398777 None:intergenic
TTCTTCGAAACGGCGTCGTT+TGG 0.281601 1_3:-7401517 Msa0100960:CDS
TTGGTTAGAAAGATTTGATT+TGG 0.282913 1_3:-7398248 Msa0100960:three_prime_UTR
AGATACTCTGAAAGAGATTA+GGG 0.311299 1_3:-7398712 Msa0100960:CDS
AAGTGTGAAAATTATATATA+TGG 0.315315 1_3:+7401822 None:intergenic
TGCCATGCACTATCTTAAAT+TGG 0.315905 1_3:-7398349 Msa0100960:three_prime_UTR
GAAGAAGAATCAATCTCTTT+AGG 0.317856 1_3:+7401543 None:intergenic
ACTTTCCGAGATCCAAAATA+TGG 0.335799 1_3:-7398769 Msa0100960:CDS
ATCAGTATGCTAACAATGTC+TGG 0.336871 1_3:-7398314 Msa0100960:three_prime_UTR
AATGTCTATGTCTTGGCTCT+TGG 0.338023 1_3:-7398132 Msa0100960:three_prime_UTR
ATGTCTATGTCTTGGCTCTT+GGG 0.345990 1_3:-7398131 Msa0100960:three_prime_UTR
TTCTATATGGAGAAGAATAA+TGG 0.349016 1_3:+7401895 None:intergenic
CATCAGACACTGTTCCAAAA+TGG 0.353087 1_3:+7398442 None:intergenic
CGGATATAGAATATTCTATA+TGG 0.363431 1_3:+7401882 None:intergenic
TTGCCCTAGAGGCCCACTTT+TGG 0.363906 1_3:+7398884 None:intergenic
TAGATACTCTGAAAGAGATT+AGG 0.367448 1_3:-7398713 Msa0100960:CDS
CCGGTAACGTTTGTTCGATC+TGG 0.378382 1_3:+7401625 None:intergenic
GAGGATTGAAAGAAAATAAC+AGG 0.383959 1_3:-7398630 Msa0100960:exon
GACAGTAGGGAGGGAAATAG+TGG 0.390036 1_3:-7400049 Msa0100960:CDS
GAAAATTATATATATGGAAG+TGG 0.390345 1_3:+7401828 None:intergenic
CAGAAGCAACGATGAAGTTC+TGG 0.393504 1_3:-7401662 Msa0100960:exon
CTGGGCCAAGAGTTCTGATA+TGG 0.418354 1_3:-7400079 Msa0100960:CDS
TCTCTCCACAGAAGAGAAAC+AGG 0.418574 1_3:+7398570 None:intergenic
CCAGATCGAACAAACGTTAC+CGG 0.425418 1_3:-7401625 Msa0100960:CDS
CATATAGAATATTCTATATC+CGG 0.434222 1_3:-7401881 Msa0100960:five_prime_UTR
AAGAAGAATATGTTATCAAA+TGG 0.435300 1_3:-7401389 Msa0100960:CDS
AATTGCAAGACAAAGTTGCC+TGG 0.440049 1_3:-7400098 Msa0100960:CDS
AAGAAATATGATAAGCGAAC+TGG 0.447869 1_3:-7399020 Msa0100960:CDS
AAGTTATTGCCATGATCTAC+TGG 0.449596 1_3:+7398541 None:intergenic
GAACACACACTGAATGAATT+CGG 0.453691 1_3:-7398480 Msa0100960:three_prime_UTR
GAACAGTGTCTGATGGTAGA+TGG 0.469667 1_3:-7398434 Msa0100960:three_prime_UTR
AAAATTATATATATGGAAGT+GGG 0.475353 1_3:+7401829 None:intergenic
GAAACGGCGTCGTTTGGATG+AGG 0.475372 1_3:-7401511 Msa0100960:CDS
TGATAAGTTTCTTTCTTACA+AGG 0.478308 1_3:-7401595 Msa0100960:CDS
TGTTTCCTGTTTCTCTTCTG+TGG 0.479255 1_3:-7398575 Msa0100960:three_prime_UTR
CAACTCCATATCAGAACTCT+TGG 0.480256 1_3:+7400074 None:intergenic
TCCTCTGCATAGCGAGACTT+TGG 0.481859 1_3:-7398655 Msa0100960:CDS
TCCCCAAAAGTGGGCCTCTA+GGG 0.484147 1_3:-7398887 Msa0100960:CDS
ATGGCTGGTTCAAGACATCT+TGG 0.485857 1_3:+7398973 None:intergenic
CTTAGTTTCATTTGCAGTCG+TGG 0.487315 1_3:+7398818 None:intergenic
TCATGGCAATAACTTGTGAT+AGG 0.493882 1_3:-7398533 Msa0100960:three_prime_UTR
CACGACTGCAAATGAAACTA+AGG 0.499694 1_3:-7398817 Msa0100960:CDS
GGAGAAATATTTCCTATGCA+TGG 0.500100 1_3:-7398180 Msa0100960:three_prime_UTR
TTCCCCAAAAGTGGGCCTCT+AGG 0.501128 1_3:-7398888 Msa0100960:CDS
AGATAGTAGCCAGTAGATCA+TGG 0.510296 1_3:-7398550 Msa0100960:three_prime_UTR
ATTACGCTCATACGGTGCAT+TGG 0.515234 1_3:+7398063 None:intergenic
GGAGTTGATGACAGTAGGGA+GGG 0.527766 1_3:-7400058 Msa0100960:CDS
TACAGGCAATGTCTATGTCT+TGG 0.531950 1_3:-7398139 Msa0100960:three_prime_UTR
TCGAACAAACGTTACCGGAT+TGG 0.531970 1_3:-7401620 Msa0100960:CDS
TAGAAAAGACAATGTTGGAC+AGG 0.535144 1_3:+7398509 None:intergenic
GCATAGCGAGACTTTGGTAG+AGG 0.537663 1_3:-7398649 Msa0100960:CDS
AGAAACTTATCACGCCAATC+CGG 0.542654 1_3:+7401606 None:intergenic
TTGAAAGAAAATAACAGGAT+AGG 0.543889 1_3:-7398625 Msa0100960:exon
TAGCCACCTTGTCACTGTAC+AGG 0.549147 1_3:-7398156 Msa0100960:three_prime_UTR
CAACATAGAAAAGACAATGT+TGG 0.556339 1_3:+7398504 None:intergenic
GATATGGAGTTGATGACAGT+AGG 0.558831 1_3:-7400063 Msa0100960:CDS
AGAATATGTTATCAAATGGA+AGG 0.560145 1_3:-7401385 Msa0100960:intron
ACACACTGAATGAATTCGGA+TGG 0.560882 1_3:-7398476 Msa0100960:three_prime_UTR
TGAAGGAGAGGTTACGAAAG+AGG 0.561251 1_3:-7401478 Msa0100960:CDS
CATATCAGAACTCTTGGCCC+AGG 0.567662 1_3:+7400080 None:intergenic
GATGAGGATGGTGGTGCTGA+AGG 0.568091 1_3:-7401495 Msa0100960:CDS
AGCGAACTGGTGCACTACTT+CGG 0.569835 1_3:-7399007 Msa0100960:CDS
TGGAGTTGATGACAGTAGGG+AGG 0.576482 1_3:-7400059 Msa0100960:CDS
ATTGCAAGACAAAGTTGCCT+GGG 0.577637 1_3:-7400097 Msa0100960:CDS
TCAGTATGCTAACAATGTCT+GGG 0.586674 1_3:-7398313 Msa0100960:three_prime_UTR
TGCCAATTTAAGATAGTGCA+TGG 0.589405 1_3:+7398347 None:intergenic
CAAGGTGGCTATCCATGCAT+AGG 0.598924 1_3:+7398168 None:intergenic
TGCATGGCACCTATAACCCT+AGG 0.599510 1_3:+7398363 None:intergenic
ATATGGAGTTGATGACAGTA+GGG 0.604313 1_3:-7400062 Msa0100960:CDS
GTTGATAAGGATTGCCCTAG+AGG 0.607707 1_3:+7398873 None:intergenic
CGGCGTCGTTTGGATGAGGA+TGG 0.613998 1_3:-7401507 Msa0100960:CDS
GCAATCCTTATCAACTAGTG+AGG 0.617605 1_3:-7398865 Msa0100960:CDS
ACCAAAGTCTCGCTATGCAG+AGG 0.618997 1_3:+7398654 None:intergenic
AAATTATATATATGGAAGTG+GGG 0.620567 1_3:+7401830 None:intergenic
AAAGTCTCGCTATGCAGAGG+AGG 0.622270 1_3:+7398657 None:intergenic
CAGACACTGTTCCAAAATGG+AGG 0.634946 1_3:+7398445 None:intergenic
ATCGCGTTTACGAAACGCAA+CGG 0.645289 1_3:+7401739 None:intergenic
TGTGCTTAACAAGCTTGTGA+AGG 0.649462 1_3:-7398937 Msa0100960:CDS
TATAGTGCACTTAACTACAC+AGG 0.649872 1_3:-7399994 Msa0100960:intron
ACTCTGAAAGAGATTAGGGG+TGG 0.655189 1_3:-7398708 Msa0100960:CDS
CGTCGTTTGGATGAGGATGG+TGG 0.657509 1_3:-7401504 Msa0100960:CDS
GGATGGTGGTGCTGAAGGAG+AGG 0.662335 1_3:-7401490 Msa0100960:CDS
GCATGGCACCTATAACCCTA+GGG 0.676500 1_3:+7398364 None:intergenic
ACATTGCCTGTACAGTGACA+AGG 0.686662 1_3:+7398150 None:intergenic
CAAGCTTGTGAAGGAGTGTG+AGG 0.691985 1_3:-7398928 Msa0100960:CDS
GATACTCTGAAAGAGATTAG+GGG 0.722491 1_3:-7398711 Msa0100960:CDS
TTGCCTGTACAGTGACAAGG+TGG 0.728898 1_3:+7398153 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTTTTTGTTGTAATA+TGG - chr1_3:7398683-7398702 Msa0100960:CDS 10.0%
!!! ATATTTTTTTTTTTACTAGT+AGG + chr1_3:7399564-7399583 None:intergenic 10.0%
!!! TTTTATTTATATCAAACATT+TGG - chr1_3:7401739-7401758 Msa0100960:five_prime_UTR 10.0%
!! AAAATTATATATATGGAAGT+GGG + chr1_3:7398180-7398199 None:intergenic 15.0%
!! AAGTGTGAAAATTATATATA+TGG + chr1_3:7398187-7398206 None:intergenic 15.0%
!!! AATAATGATAATGAGTAATT+TGG + chr1_3:7398218-7398237 None:intergenic 15.0%
!!! TTTTTAACTTTAATCGATTA+TGG - chr1_3:7398648-7398667 Msa0100960:CDS 15.0%
!! TTCTCATATTATAAATGATA+GGG + chr1_3:7400216-7400235 None:intergenic 15.0%
!! TAAACACAATATAATTAGAT+AGG - chr1_3:7400833-7400852 Msa0100960:intron 15.0%
!!! ACTTTGATATTATTTTTGTT+CGG - chr1_3:7401805-7401824 Msa0100960:five_prime_UTR 15.0%
!! AAACCGAAATTTAAATTATT+TGG - chr1_3:7401899-7401918 Msa0100960:five_prime_UTR 15.0%
!! CAACCAAATAATTTAAATTT+CGG + chr1_3:7401905-7401924 None:intergenic 15.0%
!! CATATAGAATATTCTATATC+CGG - chr1_3:7398125-7398144 Msa0100960:three_prime_UTR 20.0%
!! AGTAAATAAAAAAATGGTTC+CGG + chr1_3:7398147-7398166 None:intergenic 20.0%
!! AAATTATATATATGGAAGTG+GGG + chr1_3:7398179-7398198 None:intergenic 20.0%
!! GAAAATTATATATATGGAAG+TGG + chr1_3:7398181-7398200 None:intergenic 20.0%
!! GTTGAAATTGAGAAATTTAA+TGG - chr1_3:7398577-7398596 Msa0100960:three_prime_UTR 20.0%
!! AAGAAGAATATGTTATCAAA+TGG - chr1_3:7398617-7398636 Msa0100960:three_prime_UTR 20.0%
!! ATTTGACAAAACTATAATCT+TGG + chr1_3:7398764-7398783 None:intergenic 20.0%
!!! ATCCAATGTATGATTATTTT+TGG - chr1_3:7398992-7399011 Msa0100960:CDS 20.0%
!! GTTCTCATATTATAAATGAT+AGG + chr1_3:7400217-7400236 None:intergenic 20.0%
!! ATGAAGTTATAAGAAAATCA+TGG - chr1_3:7400369-7400388 Msa0100960:intron 20.0%
!! TATAATTAGATAGGAAAACT+AGG - chr1_3:7400842-7400861 Msa0100960:intron 20.0%
!! ATAATTAGATAGGAAAACTA+GGG - chr1_3:7400843-7400862 Msa0100960:intron 20.0%
!!! GGGAAAATTTTACATTATTA+AGG + chr1_3:7401625-7401644 None:intergenic 20.0%
!!! AATAATGTAAAATTTTCCCT+AGG - chr1_3:7401626-7401645 Msa0100960:CDS 20.0%
!!! ATAATGTAAAATTTTCCCTA+GGG - chr1_3:7401627-7401646 Msa0100960:CDS 20.0%
!! ATTATTTGGTTGCAATAAAA+AGG - chr1_3:7401913-7401932 Msa0100960:five_prime_UTR 20.0%
! TTCTATATGGAGAAGAATAA+TGG + chr1_3:7398114-7398133 None:intergenic 25.0%
! CGGATATAGAATATTCTATA+TGG + chr1_3:7398127-7398146 None:intergenic 25.0%
! TGATAAGTTTCTTTCTTACA+AGG - chr1_3:7398411-7398430 Msa0100960:three_prime_UTR 25.0%
!! AAGAGGTTAATGATTTTCTT+AGG - chr1_3:7398545-7398564 Msa0100960:three_prime_UTR 25.0%
!!! TAATGATTTTCTTAGGTTGT+TGG - chr1_3:7398552-7398571 Msa0100960:three_prime_UTR 25.0%
! AGAATATGTTATCAAATGGA+AGG - chr1_3:7398621-7398640 Msa0100960:three_prime_UTR 25.0%
! CTCAAATTATTAACATGTGT+CGG + chr1_3:7398853-7398872 None:intergenic 25.0%
! ACAATTTAGATCAGAATTGT+CGG - chr1_3:7398919-7398938 Msa0100960:CDS 25.0%
! AAGCATCATAGATTATGAAA+TGG + chr1_3:7398967-7398986 None:intergenic 25.0%
! CTCCAAAAATAATCATACAT+TGG + chr1_3:7398997-7399016 None:intergenic 25.0%
! CAAAAACTTCAACAAAATCA+CGG + chr1_3:7399050-7399069 None:intergenic 25.0%
! ACCTTAAATATCAATGAATG+TGG - chr1_3:7399155-7399174 Msa0100960:intron 25.0%
! TCCACATTCATTGATATTTA+AGG + chr1_3:7399159-7399178 None:intergenic 25.0%
! AGAATGTATGAATCTTAACA+TGG - chr1_3:7399198-7399217 Msa0100960:intron 25.0%
! GAATGTATGAATCTTAACAT+GGG - chr1_3:7399199-7399218 Msa0100960:intron 25.0%
!! CATATATTGTAATTCTGGAA+AGG - chr1_3:7399272-7399291 Msa0100960:intron 25.0%
!! TAATAATCCATTTTACTTCG+AGG + chr1_3:7399390-7399409 None:intergenic 25.0%
!! AATACGTATAATTTTACCAC+CGG - chr1_3:7399474-7399493 Msa0100960:intron 25.0%
! TACAATATACTTTGCAAATG+AGG + chr1_3:7400134-7400153 None:intergenic 25.0%
!!! GTTTTAGGGGTATTAATTAA+TGG - chr1_3:7400317-7400336 Msa0100960:intron 25.0%
!! ACCATATTTTGCAAATAAGA+TGG - chr1_3:7400428-7400447 Msa0100960:intron 25.0%
!!! ATTTATTTCTAAACTGAGTC+AGG - chr1_3:7400483-7400502 Msa0100960:intron 25.0%
!!! TTTATTTCTAAACTGAGTCA+GGG - chr1_3:7400484-7400503 Msa0100960:intron 25.0%
! GTTCTATCAAAAACCATATT+TGG - chr1_3:7400638-7400657 Msa0100960:intron 25.0%
! AACCATTAGAAAAGATTTAG+AGG + chr1_3:7400678-7400697 None:intergenic 25.0%
!! TTTTCTAATGGTTTCTCTAA+TGG - chr1_3:7400685-7400704 Msa0100960:intron 25.0%
!! TGAAAATGTTCTCCATATTT+TGG + chr1_3:7401252-7401271 None:intergenic 25.0%
! TTGAAAGAAAATAACAGGAT+AGG - chr1_3:7401381-7401400 Msa0100960:intron 25.0%
! TTGGTTAGAAAGATTTGATT+TGG - chr1_3:7401758-7401777 Msa0100960:five_prime_UTR 25.0%
GAAGAAGAATCAATCTCTTT+AGG + chr1_3:7398466-7398485 None:intergenic 30.0%
TTGAGTTTGAAGAAATCAAG+TGG - chr1_3:7399022-7399041 Msa0100960:CDS 30.0%
! TGAAGTTTTTGCTACAATCA+CGG - chr1_3:7399059-7399078 Msa0100960:intron 30.0%
!! ATGCAGTTAGTGGATTAAAT+AGG - chr1_3:7399123-7399142 Msa0100960:intron 30.0%
!! TGCAGTTAGTGGATTAAATA+GGG - chr1_3:7399124-7399143 Msa0100960:intron 30.0%
GTCAGCATATATTGTAATTC+TGG - chr1_3:7399267-7399286 Msa0100960:intron 30.0%
AACTTATCCTCGAAGTAAAA+TGG - chr1_3:7399380-7399399 Msa0100960:intron 30.0%
! ATACGTATAATTTTACCACC+GGG - chr1_3:7399475-7399494 Msa0100960:intron 30.0%
CTTGTCTGTGTTGTAAATTT+CGG - chr1_3:7399522-7399541 Msa0100960:intron 30.0%
ACTCGGCTAAAATACATTTA+CGG + chr1_3:7399602-7399621 None:intergenic 30.0%
ACATATGGCGATATAAAAGT+TGG + chr1_3:7399763-7399782 None:intergenic 30.0%
TAAGAAGTCGTTCTTAGAAA+TGG + chr1_3:7399873-7399892 None:intergenic 30.0%
AATGATAATTCAGTCCTTAG+AGG + chr1_3:7400260-7400279 None:intergenic 30.0%
! TGCAAATGAGTTACAGTTTT+AGG - chr1_3:7400302-7400321 Msa0100960:intron 30.0%
! GCAAATGAGTTACAGTTTTA+GGG - chr1_3:7400303-7400322 Msa0100960:intron 30.0%
!! CAAATGAGTTACAGTTTTAG+GGG - chr1_3:7400304-7400323 Msa0100960:intron 30.0%
! GCCATCTTATTTGCAAAATA+TGG + chr1_3:7400432-7400451 None:intergenic 30.0%
! TGGTTTTTGATAGAACACTA+TGG + chr1_3:7400634-7400653 None:intergenic 30.0%
! GACCTCTAAATCTTTTCTAA+TGG - chr1_3:7400673-7400692 Msa0100960:intron 30.0%
! GTTTCTCTAATGGTTTTCTT+AGG - chr1_3:7400695-7400714 Msa0100960:intron 30.0%
TGTAAGAAGAGTAGATCAAA+TGG + chr1_3:7400753-7400772 None:intergenic 30.0%
TTGATCTACTCTTCTTACAA+CGG - chr1_3:7400754-7400773 Msa0100960:intron 30.0%
GAAAGATGATTTCTTTGACT+AGG - chr1_3:7400883-7400902 Msa0100960:intron 30.0%
ATGATTTCTTTGACTAGGTT+GGG - chr1_3:7400888-7400907 Msa0100960:intron 30.0%
AAGAAATATGATAAGCGAAC+TGG - chr1_3:7400986-7401005 Msa0100960:intron 30.0%
TTCAAGACATCTTGGATAAA+AGG + chr1_3:7401028-7401047 None:intergenic 30.0%
! AAAAACCTCACTAGTTGATA+AGG + chr1_3:7401149-7401168 None:intergenic 30.0%
! TAGATACTCTGAAAGAGATT+AGG - chr1_3:7401293-7401312 Msa0100960:intron 30.0%
! AGATACTCTGAAAGAGATTA+GGG - chr1_3:7401294-7401313 Msa0100960:intron 30.0%
GAGGATTGAAAGAAAATAAC+AGG - chr1_3:7401376-7401395 Msa0100960:intron 30.0%
CAACATAGAAAAGACAATGT+TGG + chr1_3:7401505-7401524 None:intergenic 30.0%
AATGAATTCGGATGGAAATT+TGG - chr1_3:7401538-7401557 Msa0100960:CDS 30.0%
! AAAATTTTCCCTAGGGTTAT+AGG - chr1_3:7401634-7401653 Msa0100960:CDS 30.0%
!! AACGGTGTTTTGAATGGTTA+AGG + chr1_3:7398252-7398271 None:intergenic 35.0%
CTTCTTCTTCTTCTTCGAAA+CGG - chr1_3:7398479-7398498 Msa0100960:three_prime_UTR 35.0%
!! TGATTTTCTTAGGTTGTTGG+AGG - chr1_3:7398555-7398574 Msa0100960:three_prime_UTR 35.0%
TAATCCACTAACTGCATGAT+CGG + chr1_3:7399120-7399139 None:intergenic 35.0%
!! GCAGTTAGTGGATTAAATAG+GGG - chr1_3:7399125-7399144 Msa0100960:intron 35.0%
TATCAATGAATGTGGAACAG+AGG - chr1_3:7399163-7399182 Msa0100960:intron 35.0%
ATCAATGAATGTGGAACAGA+GGG - chr1_3:7399164-7399183 Msa0100960:intron 35.0%
TGCTGACAATTGACAAAGAA+AGG + chr1_3:7399254-7399273 None:intergenic 35.0%
! TGAGCAATCTTTTCTGATCT+TGG - chr1_3:7399319-7399338 Msa0100960:intron 35.0%
! GAGCAATCTTTTCTGATCTT+GGG - chr1_3:7399320-7399339 Msa0100960:intron 35.0%
! TACGTATAATTTTACCACCG+GGG - chr1_3:7399476-7399495 Msa0100960:intron 35.0%
CGAAATTTACAACACAGACA+AGG + chr1_3:7399524-7399543 None:intergenic 35.0%
CTTCCAGTCATAAAATCACT+CGG + chr1_3:7399619-7399638 None:intergenic 35.0%
AGGTGAAAATTGCAGACATA+TGG + chr1_3:7399723-7399742 None:intergenic 35.0%
AGGTGAAAATTGCAGACATA+TGG + chr1_3:7399723-7399742 None:intergenic 35.0%
TCTTAGAAATGGAATACGCT+AGG + chr1_3:7399862-7399881 None:intergenic 35.0%
!! ACTTCTTATGTTTGCTATGC+AGG - chr1_3:7399885-7399904 Msa0100960:intron 35.0%
!! ATATGGAGTTGATGACAGTA+GGG - chr1_3:7399944-7399963 Msa0100960:intron 35.0%
!!! AGGGAAATAGTGGATTTTCA+TGG - chr1_3:7399967-7399986 Msa0100960:intron 35.0%
TATAGTGCACTTAACTACAC+AGG - chr1_3:7400012-7400031 Msa0100960:CDS 35.0%
CAGCTTAAAATTCATGCACA+GGG + chr1_3:7400052-7400071 None:intergenic 35.0%
TCAGCTTAAAATTCATGCAC+AGG + chr1_3:7400053-7400072 None:intergenic 35.0%
! ACAGATTCTTTTTGCCTCAT+AGG - chr1_3:7400072-7400091 Msa0100960:CDS 35.0%
CCATATGTTACTATCCTATG+AGG + chr1_3:7400089-7400108 None:intergenic 35.0%
CCTCATAGGATAGTAACATA+TGG - chr1_3:7400086-7400105 Msa0100960:CDS 35.0%
TCATATGCTCAACTTAAACG+CGG + chr1_3:7400157-7400176 None:intergenic 35.0%
GAACCAATGAGAAATGAGTT+TGG - chr1_3:7400233-7400252 Msa0100960:intron 35.0%
AAGAAGAGTAGATCAAATGG+AGG + chr1_3:7400750-7400769 None:intergenic 35.0%
! TCTTCTTGTTTGTCAGTAGA+TGG - chr1_3:7400787-7400806 Msa0100960:intron 35.0%
! CTTCTTGTTTGTCAGTAGAT+GGG - chr1_3:7400788-7400807 Msa0100960:intron 35.0%
GGTGCATAAAATCAAGTGAA+TGG - chr1_3:7400809-7400828 Msa0100960:intron 35.0%
GATGATTTCTTTGACTAGGT+TGG - chr1_3:7400887-7400906 Msa0100960:intron 35.0%
!!! AGCACATCGATTTTGAAGAA+TGG + chr1_3:7401055-7401074 None:intergenic 35.0%
! AGCATTATTTTCCCCAAAAG+TGG - chr1_3:7401109-7401128 Msa0100960:intron 35.0%
! GCATTATTTTCCCCAAAAGT+GGG - chr1_3:7401110-7401129 Msa0100960:intron 35.0%
! ATCAACTAGTGAGGTTTTTG+AGG - chr1_3:7401150-7401169 Msa0100960:intron 35.0%
ACTTTCCGAGATCCAAAATA+TGG - chr1_3:7401237-7401256 Msa0100960:intron 35.0%
!! GTTCTCCATATTTTGGATCT+CGG + chr1_3:7401245-7401264 None:intergenic 35.0%
! GATACTCTGAAAGAGATTAG+GGG - chr1_3:7401295-7401314 Msa0100960:intron 35.0%
AAGTTATTGCCATGATCTAC+TGG + chr1_3:7401468-7401487 None:intergenic 35.0%
! TCATGGCAATAACTTGTGAT+AGG - chr1_3:7401473-7401492 Msa0100960:CDS 35.0%
TAGAAAAGACAATGTTGGAC+AGG + chr1_3:7401500-7401519 None:intergenic 35.0%
GAACACACACTGAATGAATT+CGG - chr1_3:7401526-7401545 Msa0100960:CDS 35.0%
TGCCATGCACTATCTTAAAT+TGG - chr1_3:7401657-7401676 Msa0100960:exon 35.0%
! TGCCAATTTAAGATAGTGCA+TGG + chr1_3:7401662-7401681 None:intergenic 35.0%
ATCAGTATGCTAACAATGTC+TGG - chr1_3:7401692-7401711 Msa0100960:five_prime_UTR 35.0%
TCAGTATGCTAACAATGTCT+GGG - chr1_3:7401693-7401712 Msa0100960:five_prime_UTR 35.0%
GGAGAAATATTTCCTATGCA+TGG - chr1_3:7401826-7401845 Msa0100960:five_prime_UTR 35.0%
!! AAACGCAACGGTGTTTTGAA+TGG + chr1_3:7398258-7398277 None:intergenic 40.0%
AGAAACTTATCACGCCAATC+CGG + chr1_3:7398403-7398422 None:intergenic 40.0%
AAATCCGATCATGCAGTTAG+TGG - chr1_3:7399113-7399132 Msa0100960:intron 40.0%
TCAATGAATGTGGAACAGAG+GGG - chr1_3:7399165-7399184 Msa0100960:intron 40.0%
! TCTTTTCTGATCTTGGGAGT+TGG - chr1_3:7399326-7399345 Msa0100960:intron 40.0%
! CTTTTCTGATCTTGGGAGTT+GGG - chr1_3:7399327-7399346 Msa0100960:intron 40.0%
! TAGCCGAGTGATTTTATGAC+TGG - chr1_3:7399613-7399632 Msa0100960:intron 40.0%
TGGTGTAACAATGCAGAACA+AGG + chr1_3:7399798-7399817 None:intergenic 40.0%
TCTCTGTGACATGGAAAAGT+TGG + chr1_3:7399818-7399837 None:intergenic 40.0%
AATTGCAAGACAAAGTTGCC+TGG - chr1_3:7399908-7399927 Msa0100960:intron 40.0%
ATTGCAAGACAAAGTTGCCT+GGG - chr1_3:7399909-7399928 Msa0100960:intron 40.0%
CAACTCCATATCAGAACTCT+TGG + chr1_3:7399935-7399954 None:intergenic 40.0%
!! GATATGGAGTTGATGACAGT+AGG - chr1_3:7399943-7399962 Msa0100960:intron 40.0%
!! AGTGGATTTTCATGGAGAGA+TGG - chr1_3:7399975-7399994 Msa0100960:intron 40.0%
GGTCCAAACTCATTTCTCAT+TGG + chr1_3:7400239-7400258 None:intergenic 40.0%
GAAATGAGTTTGGACCTCTA+AGG - chr1_3:7400243-7400262 Msa0100960:intron 40.0%
CAATGAGCTGGATCCAAATA+TGG + chr1_3:7400654-7400673 None:intergenic 40.0%
AGATTTAGAGGTCAATGAGC+TGG + chr1_3:7400666-7400685 None:intergenic 40.0%
! TTCCTCTACCTCTTTTAACC+TGG - chr1_3:7400720-7400739 Msa0100960:intron 40.0%
ATAGGAAAACTAGGGTGTGT+AGG - chr1_3:7400851-7400870 Msa0100960:intron 40.0%
!!! CATCGATTTTGAAGAATGGC+TGG + chr1_3:7401051-7401070 None:intergenic 40.0%
TGTGCTTAACAAGCTTGTGA+AGG - chr1_3:7401069-7401088 Msa0100960:intron 40.0%
GCAATCCTTATCAACTAGTG+AGG - chr1_3:7401141-7401160 Msa0100960:intron 40.0%
CTTAGTTTCATTTGCAGTCG+TGG + chr1_3:7401191-7401210 None:intergenic 40.0%
CACGACTGCAAATGAAACTA+AGG - chr1_3:7401189-7401208 Msa0100960:intron 40.0%
! GGATCTCGGAAAGTTCTTTT+GGG + chr1_3:7401231-7401250 None:intergenic 40.0%
! TGGATCTCGGAAAGTTCTTT+TGG + chr1_3:7401232-7401251 None:intergenic 40.0%
! TGTTTCCTGTTTCTCTTCTG+TGG - chr1_3:7401431-7401450 Msa0100960:CDS 40.0%
AGATAGTAGCCAGTAGATCA+TGG - chr1_3:7401456-7401475 Msa0100960:CDS 40.0%
ACACACTGAATGAATTCGGA+TGG - chr1_3:7401530-7401549 Msa0100960:CDS 40.0%
! TGGAAATTTGGCCTCCATTT+TGG - chr1_3:7401550-7401569 Msa0100960:CDS 40.0%
CATCAGACACTGTTCCAAAA+TGG + chr1_3:7401567-7401586 None:intergenic 40.0%
!!! CATTTTGGAACAGTGTCTGA+TGG - chr1_3:7401565-7401584 Msa0100960:CDS 40.0%
TACAGGCAATGTCTATGTCT+TGG - chr1_3:7401867-7401886 Msa0100960:five_prime_UTR 40.0%
! AATGTCTATGTCTTGGCTCT+TGG - chr1_3:7401874-7401893 Msa0100960:five_prime_UTR 40.0%
! ATGTCTATGTCTTGGCTCTT+GGG - chr1_3:7401875-7401894 Msa0100960:five_prime_UTR 40.0%
ATCGCGTTTACGAAACGCAA+CGG + chr1_3:7398270-7398289 None:intergenic 45.0%
CAGAAGCAACGATGAAGTTC+TGG - chr1_3:7398344-7398363 Msa0100960:three_prime_UTR 45.0%
CCAGATCGAACAAACGTTAC+CGG - chr1_3:7398381-7398400 Msa0100960:three_prime_UTR 45.0%
TCGAACAAACGTTACCGGAT+TGG - chr1_3:7398386-7398405 Msa0100960:three_prime_UTR 45.0%
TGAAGGAGAGGTTACGAAAG+AGG - chr1_3:7398528-7398547 Msa0100960:three_prime_UTR 45.0%
CAATGAATGTGGAACAGAGG+GGG - chr1_3:7399166-7399185 Msa0100960:intron 45.0%
TGGTGTGACTATGCAGAACA+AGG + chr1_3:7399743-7399762 None:intergenic 45.0%
! CAAGCCTTATCCCACTAAGT+GGG - chr1_3:7400584-7400603 Msa0100960:intron 45.0%
! AAGCCTTATCCCACTAAGTG+GGG - chr1_3:7400585-7400604 Msa0100960:intron 45.0%
AGCCAGGTTAAAAGAGGTAG+AGG + chr1_3:7400725-7400744 None:intergenic 45.0%
TCTTCTTACAACGGCGTCTA+CGG - chr1_3:7400763-7400782 Msa0100960:intron 45.0%
TCTTCTCTCATTCTGTGTGC+AGG - chr1_3:7400947-7400966 Msa0100960:intron 45.0%
TCTCATTCTGTGTGCAGGTT+TGG - chr1_3:7400952-7400971 Msa0100960:intron 45.0%
! ATGGCTGGTTCAAGACATCT+TGG + chr1_3:7401036-7401055 None:intergenic 45.0%
! GTTGATAAGGATTGCCCTAG+AGG + chr1_3:7401136-7401155 None:intergenic 45.0%
!!! CGGAAAGTTCTTTTGGGACA+TGG + chr1_3:7401225-7401244 None:intergenic 45.0%
! ACTCTGAAAGAGATTAGGGG+TGG - chr1_3:7401298-7401317 Msa0100960:intron 45.0%
TCTCTCCACAGAAGAGAAAC+AGG + chr1_3:7401439-7401458 None:intergenic 45.0%
CAGACACTGTTCCAAAATGG+AGG + chr1_3:7401564-7401583 None:intergenic 45.0%
GAACAGTGTCTGATGGTAGA+TGG - chr1_3:7401572-7401591 Msa0100960:CDS 45.0%
ACATTGCCTGTACAGTGACA+AGG + chr1_3:7401859-7401878 None:intergenic 45.0%
!! TAAAAAAGTAAATAAAAAAA+TGG + chr1_3:7398153-7398172 None:intergenic 5.0%
CCGGTAACGTTTGTTCGATC+TGG + chr1_3:7398384-7398403 None:intergenic 50.0%
TTCTTCGAAACGGCGTCGTT+TGG - chr1_3:7398489-7398508 Msa0100960:three_prime_UTR 50.0%
CATGGGTCGTTGTTTGCTCT+TGG - chr1_3:7399216-7399235 Msa0100960:intron 50.0%
ATCTTGGGAGTTGGGACTGT+TGG - chr1_3:7399335-7399354 Msa0100960:intron 50.0%
!! CAACACAGACAAGGAGCATC+AGG + chr1_3:7399515-7399534 None:intergenic 50.0%
AGACGTGACTCCTTGAGATG+TGG - chr1_3:7399835-7399854 Msa0100960:intron 50.0%
TACGCTAGGTCCACATCTCA+AGG + chr1_3:7399848-7399867 None:intergenic 50.0%
CATATCAGAACTCTTGGCCC+AGG + chr1_3:7399929-7399948 None:intergenic 50.0%
! CTGGGCCAAGAGTTCTGATA+TGG - chr1_3:7399927-7399946 Msa0100960:intron 50.0%
!! TGGAGTTGATGACAGTAGGG+AGG - chr1_3:7399947-7399966 Msa0100960:intron 50.0%
!! GGAGTTGATGACAGTAGGGA+GGG - chr1_3:7399948-7399967 Msa0100960:intron 50.0%
GACAGTAGGGAGGGAAATAG+TGG - chr1_3:7399957-7399976 Msa0100960:intron 50.0%
CTTAGTGGGATAAGGCTTGG+TGG + chr1_3:7400583-7400602 None:intergenic 50.0%
CCACTTAGTGGGATAAGGCT+TGG + chr1_3:7400586-7400605 None:intergenic 50.0%
! CCAAGCCTTATCCCACTAAG+TGG - chr1_3:7400583-7400602 Msa0100960:intron 50.0%
CTTATCCCACTAAGTGGGGT+CGG - chr1_3:7400589-7400608 Msa0100960:intron 50.0%
GAGGAGAGCCAGGTTAAAAG+AGG + chr1_3:7400731-7400750 None:intergenic 50.0%
AGATCAAATGGAGGAGAGCC+AGG + chr1_3:7400741-7400760 None:intergenic 50.0%
CTTCTTACAACGGCGTCTAC+GGG - chr1_3:7400764-7400783 Msa0100960:intron 50.0%
ACTAGGGTGTGTAGGACAGT+AGG - chr1_3:7400859-7400878 Msa0100960:intron 50.0%
AGCGAACTGGTGCACTACTT+CGG - chr1_3:7400999-7401018 Msa0100960:intron 50.0%
! CAAGCTTGTGAAGGAGTGTG+AGG - chr1_3:7401078-7401097 Msa0100960:intron 50.0%
AAAGTCTCGCTATGCAGAGG+AGG + chr1_3:7401352-7401371 None:intergenic 50.0%
TCCTCTGCATAGCGAGACTT+TGG - chr1_3:7401351-7401370 Msa0100960:intron 50.0%
ACCAAAGTCTCGCTATGCAG+AGG + chr1_3:7401355-7401374 None:intergenic 50.0%
GCATAGCGAGACTTTGGTAG+AGG - chr1_3:7401357-7401376 Msa0100960:intron 50.0%
GCATGGCACCTATAACCCTA+GGG + chr1_3:7401645-7401664 None:intergenic 50.0%
TGCATGGCACCTATAACCCT+AGG + chr1_3:7401646-7401665 None:intergenic 50.0%
CAAGGTGGCTATCCATGCAT+AGG + chr1_3:7401841-7401860 None:intergenic 50.0%
TAGCCACCTTGTCACTGTAC+AGG - chr1_3:7401850-7401869 Msa0100960:five_prime_UTR 50.0%
TTGCCTGTACAGTGACAAGG+TGG + chr1_3:7401856-7401875 None:intergenic 50.0%
GAAACGGCGTCGTTTGGATG+AGG - chr1_3:7398495-7398514 Msa0100960:three_prime_UTR 55.0%
CGTCGTTTGGATGAGGATGG+TGG - chr1_3:7398502-7398521 Msa0100960:three_prime_UTR 55.0%
! GATGAGGATGGTGGTGCTGA+AGG - chr1_3:7398511-7398530 Msa0100960:three_prime_UTR 55.0%
GGGTCGTTGTTTGCTCTTGG+TGG - chr1_3:7399219-7399238 Msa0100960:intron 55.0%
TTGGGAGTTGGGACTGTTGG+TGG - chr1_3:7399338-7399357 Msa0100960:intron 55.0%
! GAGTTGGGACTGTTGGTGGA+AGG - chr1_3:7399342-7399361 Msa0100960:intron 55.0%
!! ACAAGGAGCATCAGGCCCTT+GGG + chr1_3:7399507-7399526 None:intergenic 55.0%
GGAGTCACGTCTCTGTGACA+TGG + chr1_3:7399827-7399846 None:intergenic 55.0%
CGACCCCACTTAGTGGGATA+AGG + chr1_3:7400591-7400610 None:intergenic 55.0%
TGTAGCCGACCCCACTTAGT+GGG + chr1_3:7400597-7400616 None:intergenic 55.0%
ATGTAGCCGACCCCACTTAG+TGG + chr1_3:7400598-7400617 None:intergenic 55.0%
ACTAAGTGGGGTCGGCTACA+TGG - chr1_3:7400597-7400616 Msa0100960:intron 55.0%
TTCCCCAAAAGTGGGCCTCT+AGG - chr1_3:7401118-7401137 Msa0100960:intron 55.0%
TCCCCAAAAGTGGGCCTCTA+GGG - chr1_3:7401119-7401138 Msa0100960:intron 55.0%
!! TGCCCTAGAGGCCCACTTTT+GGG + chr1_3:7401124-7401143 None:intergenic 55.0%
! TTGCCCTAGAGGCCCACTTT+TGG + chr1_3:7401125-7401144 None:intergenic 55.0%
CGGCGTCGTTTGGATGAGGA+TGG - chr1_3:7398499-7398518 Msa0100960:three_prime_UTR 60.0%
! GGATGGTGGTGCTGAAGGAG+AGG - chr1_3:7398516-7398535 Msa0100960:three_prime_UTR 60.0%
!! GACAAGGAGCATCAGGCCCT+TGG + chr1_3:7399508-7399527 None:intergenic 60.0%
!! GCCCTAGAGGCCCACTTTTG+GGG + chr1_3:7401123-7401142 None:intergenic 60.0%
TACCACCGGGGTCTAGCCCA+AGG - chr1_3:7399488-7399507 Msa0100960:intron 65.0%
ACCACCGGGGTCTAGCCCAA+GGG - chr1_3:7399489-7399508 Msa0100960:intron 65.0%
! CAGGCCCTTGGGCTAGACCC+CGG + chr1_3:7399496-7399515 None:intergenic 70.0%
! GCCCTTGGGCTAGACCCCGG+TGG + chr1_3:7399493-7399512 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 7398076 7401952 7398076 ID=Msa0100960;Name=Msa0100960
chr1_3 mRNA 7398076 7401952 7398076 ID=Msa0100960-mRNA-1;Parent=Msa0100960;Name=Msa0100960-mRNA-1;_AED=0.01;_eAED=0.01;_QI=279|1|1|1|1|1|3|568|276
chr1_3 exon 7398076 7399059 7398076 ID=Msa0100960-mRNA-1:exon:2927;Parent=Msa0100960-mRNA-1
chr1_3 exon 7399995 7400121 7399995 ID=Msa0100960-mRNA-1:exon:2926;Parent=Msa0100960-mRNA-1
chr1_3 exon 7401386 7401952 7401386 ID=Msa0100960-mRNA-1:exon:2925;Parent=Msa0100960-mRNA-1
chr1_3 five_prime_UTR 7401674 7401952 7401674 ID=Msa0100960-mRNA-1:five_prime_utr;Parent=Msa0100960-mRNA-1
chr1_3 CDS 7401386 7401673 7401386 ID=Msa0100960-mRNA-1:cds;Parent=Msa0100960-mRNA-1
chr1_3 CDS 7399995 7400121 7399995 ID=Msa0100960-mRNA-1:cds;Parent=Msa0100960-mRNA-1
chr1_3 CDS 7398644 7399059 7398644 ID=Msa0100960-mRNA-1:cds;Parent=Msa0100960-mRNA-1
chr1_3 three_prime_UTR 7398076 7398643 7398076 ID=Msa0100960-mRNA-1:three_prime_utr;Parent=Msa0100960-mRNA-1
Gene Sequence

>Msa0100960

ATGAAGTTCTGGAAGATTCTCAACAACCAGATCGAACAAACGTTACCGGATTGGCGTGATAAGTTTCTTTCTTACAAGGATCTTAAGAAACAGTTAAAACTCATTGTTCCTAAAGAGATTGATTCTTCTTCTTCTTCTTCGAAACGGCGTCGTTTGGATGAGGATGGTGGTGCTGAAGGAGAGGTTACGAAAGAGGTTAATGATTTTCTTAGGTTGTTGGAGGTTGAAATTGAGAAATTTAATGGTTTTTTTGTTGAGAAAGAAGAAGAATATGTTATCAAATGGAAGGAATTGCAAGACAAAGTTGCCTGGGCCAAGAGTTCTGATATGGAGTTGATGACAGTAGGGAGGGAAATAGTGGATTTTCATGGAGAGATGGTTTTGTTAGAGAACTATAGTGCACTTAACTACACAGGTTTGGTTAAGATAATAAAGAAATATGATAAGCGAACTGGTGCACTACTTCGGTTACCTTTTATCCAAGATGTCTTGAACCAGCCATTCTTCAAAATCGATGTGCTTAACAAGCTTGTGAAGGAGTGTGAGGTGATGCTAAGCATTATTTTCCCCAAAAGTGGGCCTCTAGGGCAATCCTTATCAACTAGTGAGGTTTTTGAGGAAGTTGCACGTGAGACCACGACTGCAAATGAAACTAAGGAGACACTAGACCATGTCCCAAAAGAACTTTCCGAGATCCAAAATATGGAGAACATTTTCATCAAACTAACTACATCAGCACTAGATACTCTGAAAGAGATTAGGGGTGGAAGCTCAACTGTAAGCATATACTCATTGCCTCCTCTGCATAGCGAGACTTTGGTAGAGGATTGA
Protein sequence

>Msa0100960

MKFWKILNNQIEQTLPDWRDKFLSYKDLKKQLKLIVPKEIDSSSSSSKRRRLDEDGGAEGEVTKEVNDFLRLLEVEIEKFNGFFVEKEEEYVIKWKELQDKVAWAKSSDMELMTVGREIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALLRLPFIQDVLNQPFFKIDVLNKLVKECEVMLSIIFPKSGPLGQSLSTSEVFEEVARETTTANETKETLDHVPKELSEIQNMENIFIKLTTSALDTLKEIRGGSSTVSIYSLPPLHSETLVED*