AlfalfaGEDB | Msa0101600

M. sativa cultivar zhongmu-4 / Msa0101600

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0101600	XP_013466025.1	92.130	216	17	0	1	216	1	216	2.63e-142	412

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0101600	sp\|Q8RYD1\|REM16_ARATH	50.658	152	67	4	8	157	11	156	3.71e-43	150

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0101600	A0A072VDG5	92.130	216	17	0	1	216	1	216	1.26e-142	412

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TFs/TRs:

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Gene ID	Type	Classification
Msa0101600	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0101600	Msa0145450	0.802491	5.471531e-49	-8.615850e-47
Msa0101600	Msa0867210	0.869797	2.364557e-66	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0101600	MtrunA17_Chr1g0154191	92.130	216	17	0	1	216	1	216	2.42e-146	412
Msa0101600	MtrunA17_Chr1g0154171	73.853	218	44	4	5	216	6	216	2.10e-105	308
Msa0101600	MtrunA17_Chr1g0154241	63.052	249	49	5	1	211	1	244	7.57e-98	285
Msa0101600	MtrunA17_Chr1g0154211	59.919	247	66	3	2	216	3	248	1.81e-93	279
Msa0101600	MtrunA17_Chr1g0154251	60.776	232	48	5	3	192	8	238	1.98e-89	272
Msa0101600	MtrunA17_Chr1g0154131	71.875	192	29	4	6	196	168	335	2.21e-88	268
Msa0101600	MtrunA17_Chr1g0154131	69.643	168	50	1	4	171	8	174	1.06e-76	238
Msa0101600	MtrunA17_Chr1g0154111	74.432	176	41	3	4	178	8	180	1.52e-86	267
Msa0101600	MtrunA17_Chr1g0154111	55.500	200	67	6	37	216	368	565	2.05e-53	180
Msa0101600	MtrunA17_Chr1g0154101	77.914	163	36	0	3	165	6	168	4.10e-85	268

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0101600	AT4G33280.2	53.788	132	58	2	8	138	11	140	3.53e-45	152
Msa0101600	AT4G33280.1	50.658	152	67	4	8	157	11	156	3.77e-44	150
Msa0101600	AT4G33280.3	53.788	132	58	2	8	138	11	140	4.29e-44	152
Msa0101600	AT4G34400.2	37.273	110	63	3	15	121	8	114	4.19e-19	85.1
Msa0101600	AT4G34400.1	37.273	110	63	3	15	121	8	114	4.19e-19	85.1
Msa0101600	AT5G60130.5	41.573	89	47	2	36	119	27	115	9.83e-15	71.2
Msa0101600	AT5G60130.3	41.573	89	47	2	36	119	1	89	1.15e-14	71.6
Msa0101600	AT5G66980.1	45.122	82	41	1	34	111	21	102	1.46e-14	71.6
Msa0101600	AT5G60130.2	41.573	89	47	2	36	119	27	115	1.49e-14	71.2
Msa0101600	AT5G60130.4	41.573	89	47	2	36	119	24	112	1.77e-14	71.2

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Find 88 sgRNAs with CRISPR-Local

Find 287 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGGAGGTAAATGATT+GGG	0.509472	1_3:-8237634	None:intergenic
AAACCAACACATATGCTAAA+CGG	0.480555	1_3:+8237986	Msa0101600:three_prime_UTR
AAACCCTTCAACAGTTCACG+AGG	0.670720	1_3:-8234548	None:intergenic
AAAGGTACAAAGCTAAAGTA+GGG	0.563924	1_3:-8234575	None:intergenic
AACAAAATATGGAAAAGAGG+AGG	0.585641	1_3:-8237646	None:intergenic
AACTCCACTAGGACCTTTAA+GGG	0.479312	1_3:-8235770	None:intergenic
AACTCTATCCCATATGCTAA+AGG	0.447966	1_3:+8237952	Msa0101600:three_prime_UTR
AAGGTCCTAGTGGAGTTGTG+TGG	0.624686	1_3:+8235776	Msa0101600:CDS
AAGGTCTTGACCAGCAAAAT+GGG	0.444958	1_3:+8238142	Msa0101600:three_prime_UTR
AAGTTGTTGTTGAAAATCGG+TGG	0.662291	1_3:-8234725	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAAATGGATGAACTGAAAA+TGG	-	chr1_3:8234687-8234706	None:intergenic	30.0%
TATGAAGAAATTGGGTGAAA+TGG	-	chr1_3:8234702-8234721	None:intergenic	30.0%
CTAATATGAACACTTCAGAA+GGG	-	chr1_3:8234806-8234825	None:intergenic	30.0%
ATTTACACAGATAGTGTTTG+CGG	-	chr1_3:8235226-8235245	None:intergenic	30.0%
CATTAATGACCTATGAAGTA+CGG	+	chr1_3:8235289-8235308	Msa0101600:intron	30.0%
ACTATATTACAACTGTGTGA+GGG	+	chr1_3:8235478-8235497	Msa0101600:intron	30.0%
AATTTGAACTTTGTACCTTG+AGG	+	chr1_3:8235501-8235520	Msa0101600:intron	30.0%
ACATGATTTACATACACTTG+AGG	-	chr1_3:8235553-8235572	None:intergenic	30.0%
GATACTGTTTACTTCACAAA+CGG	+	chr1_3:8235823-8235842	Msa0101600:CDS	30.0%
AATAAATTTACGCTTGTCCT+CGG	+	chr1_3:8236183-8236202	Msa0101600:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	8234509	8238404	8234509	ID=Msa0101600;Name=Msa0101600
chr1_3	mRNA	8234509	8238404	8234509	ID=Msa0101600-mRNA-1;Parent=Msa0101600;Name=Msa0101600-mRNA-1;_AED=0.33;_eAED=0.33;_QI=131\|0.66\|0.75\|1\|1\|1\|4\|666\|216
chr1_3	exon	8234509	8234747	8234509	ID=Msa0101600-mRNA-1:exon:3244;Parent=Msa0101600-mRNA-1
chr1_3	exon	8235715	8236255	8235715	ID=Msa0101600-mRNA-1:exon:3245;Parent=Msa0101600-mRNA-1
chr1_3	exon	8237586	8237710	8237586	ID=Msa0101600-mRNA-1:exon:3246;Parent=Msa0101600-mRNA-1
chr1_3	exon	8237862	8238404	8237862	ID=Msa0101600-mRNA-1:exon:3247;Parent=Msa0101600-mRNA-1
chr1_3	five_prime_UTR	8234509	8234639	8234509	ID=Msa0101600-mRNA-1:five_prime_utr;Parent=Msa0101600-mRNA-1
chr1_3	CDS	8234640	8234747	8234640	ID=Msa0101600-mRNA-1:cds;Parent=Msa0101600-mRNA-1
chr1_3	CDS	8235715	8236255	8235715	ID=Msa0101600-mRNA-1:cds;Parent=Msa0101600-mRNA-1
chr1_3	CDS	8237586	8237587	8237586	ID=Msa0101600-mRNA-1:cds;Parent=Msa0101600-mRNA-1
chr1_3	three_prime_UTR	8237588	8237710	8237588	ID=Msa0101600-mRNA-1:three_prime_utr;Parent=Msa0101600-mRNA-1
chr1_3	three_prime_UTR	8237862	8238404	8237862	ID=Msa0101600-mRNA-1:three_prime_utr;Parent=Msa0101600-mRNA-1

Gene Sequence

>Msa0101600

ATGGTAGGAAGCACCAAATCTTGGGAGGAAGAGATTTACTGGAACCATTTTCAGTTCATCCATTTCACCCAATTTCTTCATAGCACCACCGATTTTCAACAACAACTTGCACTTCCTAAAACATTTTCAGACAATTTAAAGAAGAAGTTGCCAGAAAATGTGACCCTTAAAGGTCCTAGTGGAGTTGTGTGGAATATAGAGCTGACAACTGAAGATGATACTGTTTACTTCACAAACGGTTGGCAACGATTTGTGAATGATCACTCTTTGAAAGAGAGTGATTTCCTTTTCTTCAAGTACAATGGCGAATCGTTGTTTGAAGTTTTAATCTTTGATGGACTTAGTTTCTGTGAGAAGGCAGCTTCTTATTTTGTTGGAAAATGTGGTCATGCTCAAACCGAACAAGGGGGTATCAAGGCCAAGGACACAAACAAGTCTGTGGAAGAAATCAATACTACTTTTGATGCGGGTGTGGAGTCTGCTTCCCCTGAGAAGTGCATGGCTGATGCAGTCACTAAGACCACCCCTGTTGCAGCTCCCTCCCAATTAACTGGTAAAAGGATCAAGAAGCCTGTTAATAAATTTACGCTTGTCCTCGGATTACCAAAAGCAGCTACTTCTCATAAGAGAGCACATGATTTGGTGGCCTAG

Protein sequence

>Msa0101600

MVGSTKSWEEEIYWNHFQFIHFTQFLHSTTDFQQQLALPKTFSDNLKKKLPENVTLKGPSGVVWNIELTTEDDTVYFTNGWQRFVNDHSLKESDFLFFKYNGESLFEVLIFDGLSFCEKAASYFVGKCGHAQTEQGGIKAKDTNKSVEEINTTFDAGVESASPEKCMADAVTKTTPVAAPSQLTGKRIKKPVNKFTLVLGLPKAATSHKRAHDLVA*